FvH4_6g37091

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001653
Physical Location & Seq
Forward (+)
29161764 .. 29167454
5691 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37091.t1

Sequence Viewer

Length: 1449 bp
ATGGGTTCCAGATTCCCATCTCATCAACTCAGCAATGGCCTTTATGTATCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCGACGCCCTATACTGGTGGAGATATCAAGAAGTCAGGAGAACTAGGAAAGATGTTTGATATCCCTACAGATGGCTCCAAGTCCAGAAAATCTGGACCTATAACTGGTGCTTCTTCTAGGACTGGATCATTTGGAGGTGCCGCTTCACATTCAGGACCAATCCCTAATGCTTCAGCTCGTGCCGGCTATACTACATCAGGACCCATATTATCTGGAGGAACTGGTTCAGCAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCCATAAAGAAGTCATCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCTGGGCCTCTTCCCCCTGTTCTCCCTACCACAGGTCTCATTACTTCTGGACCCATTTCTTCCGGCCCACTAAATGCTTCTGGTGCCCCTCGAAAAGTTTCTGGTCCTTTGGAGTCAATGGGATCAATGAAAGTACAAGGATCATCTATTGTTCACAATCAGGCCATTACTACTCTTAGCCAAGATGATGAGTTTTCATTTAGGAAAAACTTCCCTAAGCTAATATTATGGTCTCTGATTCTTCTCTTCGTGATGGGGTTCATAGCTGGTGGTTTTATTTTCGGTGCAGTCCACAATCCAATACTCCTCATCGTGGTTGTGATCCTTTTCAGTGCAGTTGCTACACTATTCACATGGAATACTTATTGGGGAAGAAGAGCTATTATAGGTTATATCGGTAGTTATTCGGATTCTGAGCTCAGAACTGCCAAGAATGGACAATTTGTGAAGGTGTCAGGGGTGGTTACTTGTGGTAATGTGCCTCTTGAGTCATCCTTTCAAAAGGTTCCTAGATGTGTGTATACCTCCACAAGTTTATATGAGTATCGAGGATGGGATTCAAAAGCTGCCAACACTACACATCGTCGTTTTTCTTGGGGGCTCAGATCCATGGAAAGACGTGTGGTAGACTTCTACATCTCTGATTTCCAATCTGGGTTGAGAGCTTTGGTTAAGACAGGCTCTGGAGCAAGGGTGACTCCGTATGTTGATGATTCAATTGTCATTGATGTGAATCCCATTAAGGAAGAGTTGTCTCCAGAGTTTATTAGGTGGTTGGGAGAGAGAAACCTTTCAAGTGACGATCGTGTGATGCGGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTTAGTGTAATGGGCGTCGTCCAGAGAAATGAAAATGTGTTGATGATTGTTCCTCCACCTGAGCCGATTACTACTGGATGCCAATGGGCCAAATGTATCTTCCCAGCTAGCCTTGAGGGCATTGTTTTGAGATGCGATGATGCATCAAAGAACGATGCCATACCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

483

Amino Acids

51.58

Weight (kDa)

9.62

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 236, 512
AccI GTMKAC 2 cut(s) 950, 1056
AciI CCGC 3 cut(s) 240, 421, 1243
AclWI GGATC 5 cut(s) 232, 559, 577, 745, 1029
AcoI YGGCCR 2 cut(s) 56, 418
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 255
AcyI GRCGYC 2 cut(s) 104, 1293
AfaI GTAC 2 cut(s) 564, 1259
AfiI CCNNNNNNNGG 6 cut(s) 113, 170, 203, 398, 461, 742
AflIII ACRYGT 1 cut(s) 1048
AgsI TTSAA 5 cut(s) 929, 990, 1146, 1224, 1249
AjiI CACGTC 1 cut(s) 1049
AjuI GAANNNNNNNTTGG 2 cut(s) 1361, 1393
AluBI AGCT 9 cut(s) 93, 275, 649, 695, 809, 847, 995, 1094, 1385
AluI AGCT 9 cut(s) 93, 275, 649, 695, 809, 847, 995, 1094, 1385
Alw21I GWGCWC 2 cut(s) 95, 849
Alw26I GTCTC 3 cut(s) 470, 666, 1188
AlwI GGATC 5 cut(s) 232, 559, 577, 745, 1029
AlwNI CAGNNNCTG 1 cut(s) 1112
AoxI GGCC 8 cut(s) 37, 52, 56, 418, 434, 493, 591, 1365
ApeKI GCWGC 1 cut(s) 995
ApoI RAATTY 1 cut(s) 340
Asp700I GAANNNNTTC 4 cut(s) 322, 526, 638, 1219
AsuHPI GGTGA 1 cut(s) 1135
AsuNHI GCTAGC 1 cut(s) 1385
AvaII GGWCC 7 cut(s) 194, 254, 299, 347, 389, 479, 533
BaeGI GKGCMC 1 cut(s) 517
BanI GGYRCC 2 cut(s) 236, 512
BanII GRGCYC 3 cut(s) 95, 849, 1032
BauI CACGAG 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 95, 849
BbvI GCAGC 1 cut(s) 982
BccI CCATC 4 cut(s) 25, 164, 676, 975
BcoDI GTCTC 3 cut(s) 470, 666, 1188
BfaI CTAG 4 cut(s) 143, 216, 939, 1386
BfmI CTRYAG 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 1395
BisI GCNGC 3 cut(s) 240, 421, 996
BlsI GCNGC 3 cut(s) 241, 422, 997
Bme18I GGWCC 7 cut(s) 194, 254, 299, 347, 389, 479, 533
BmgBI CACGTC 1 cut(s) 1049
BmiI GGNNCC 8 cut(s) 7, 175, 238, 301, 369, 481, 514, 936
BmsI GCATC 6 cut(s) 1230, 1346, 1400, 1408, 1423, 1430
BmtI GCTAGC 1 cut(s) 1389
BpmI CTGGAG 3 cut(s) 333, 1134, 1170
Bpu10I CCTNAGC 2 cut(s) 645, 1338
BpuEI CTTGAG 2 cut(s) 935, 1412
BsaBI GATNNNNATC 2 cut(s) 16, 1161
BsaHI GRCGYC 2 cut(s) 104, 1293
BsaI GGTCTC 2 cut(s) 470, 666
BsaJI CCNNGG 1 cut(s) 1038
BsaXI ACNNNNNCTCC 2 cut(s) 1107, 1137
Bsc4I CCNNNNNNNGG 6 cut(s) 113, 170, 203, 398, 461, 742
Bse118I RCCGGY 2 cut(s) 54, 281
Bse1I ACTGG 7 cut(s) 118, 208, 226, 325, 421, 1357, 1442
Bse3DI GCAATG 1 cut(s) 40
Bse8I GATNNNNATC 2 cut(s) 16, 1161
BseDI CCNNGG 1 cut(s) 1038
BseGI GGATG 3 cut(s) 920, 986, 1361
BseJI GATNNNNATC 2 cut(s) 16, 1161
BseLI CCNNNNNNNGG 6 cut(s) 113, 170, 203, 398, 461, 742
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 6 cut(s) 43, 108, 834, 862, 1045, 1329
BseNI ACTGG 7 cut(s) 118, 208, 226, 325, 421, 1357, 1442
BseRI GAGGAG 1 cut(s) 725
BseSI GKGCMC 1 cut(s) 517
BseXI GCAGC 1 cut(s) 982
BseYI CCCAGC 1 cut(s) 1381
BsgI GTGCAG 2 cut(s) 735, 783
Bsh1285I CGRYCG 1 cut(s) 1234
BshFI GGCC 8 cut(s) 39, 54, 58, 420, 436, 495, 593, 1367
BshNI GGYRCC 2 cut(s) 236, 512
BsiEI CGRYCG 1 cut(s) 1234
BsiHKAI GWGCWC 2 cut(s) 95, 849
BsiSI CCGG 3 cut(s) 55, 282, 492
BslI CCNNNNNNNGG 6 cut(s) 113, 170, 203, 398, 461, 742
BsmAI GTCTC 3 cut(s) 470, 666, 1188
BsnI GGCC 8 cut(s) 39, 54, 58, 420, 436, 495, 593, 1367
Bso31I GGTCTC 2 cut(s) 470, 666
Bsp1286I GDGCHC 4 cut(s) 95, 517, 849, 1032
Bsp143I GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 3 cut(s) 240, 421, 1243
BspANI GGCC 8 cut(s) 39, 54, 58, 420, 436, 495, 593, 1367
BspCNI CTCAG 6 cut(s) 42, 107, 835, 861, 1044, 1330
BspLI GGNNCC 8 cut(s) 7, 175, 238, 301, 369, 481, 514, 936
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 5 cut(s) 232, 559, 577, 745, 1029
BspQI GCTCTTC 1 cut(s) 799
BspT107I GGYRCC 2 cut(s) 236, 512
BspTNI GGTCTC 2 cut(s) 470, 666
BsrDI GCAATG 1 cut(s) 40
BsrFI RCCGGY 2 cut(s) 54, 281
BsrI ACTGG 7 cut(s) 118, 208, 226, 325, 421, 1357, 1442
BssAI RCCGGY 2 cut(s) 54, 281
BssECI CCNNGG 1 cut(s) 1038
BssMI GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
BssNAI GTATAC 1 cut(s) 951
BssNI GRCGYC 2 cut(s) 104, 1293
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 1038
Bst1107I GTATAC 1 cut(s) 951
Bst2BI CACGAG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 1279
Bst6I CTCTTC 4 cut(s) 444, 680, 799, 1170
BstACI GRCGYC 2 cut(s) 104, 1293
BstC8I GCNNGC 3 cut(s) 56, 283, 1387
BstDEI CTNAG 8 cut(s) 29, 94, 605, 645, 843, 848, 1031, 1338
BstDSI CCRYGG 1 cut(s) 1038
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 3 cut(s) 920, 986, 1361
BstKTI GATC 6 cut(s) 227, 554, 572, 753, 1037, 1234
BstMAI GTCTC 3 cut(s) 470, 666, 1188
BstMBI GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
BstMCI CGRYCG 1 cut(s) 1234
BstMWI GCNNNNNNNGC 2 cut(s) 512, 1395
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 517
BstV1I GCAGC 1 cut(s) 982
BstX2I RGATCY 1 cut(s) 1034
BstXI CCANNNNNNTGG 1 cut(s) 431
BstYI RGATCY 1 cut(s) 1034
BstZ17I GTATAC 1 cut(s) 951
BsuRI GGCC 8 cut(s) 39, 54, 58, 420, 436, 495, 593, 1367
BtgI CCRYGG 1 cut(s) 1038
BtgZI GCGATG 1 cut(s) 1428
BtrI CACGTC 1 cut(s) 1049
BtsCI GGATG 3 cut(s) 920, 986, 1361
BtsIMutI CAGTG 2 cut(s) 350, 766
Cac8I GCNNGC 3 cut(s) 56, 283, 1387
CaiI CAGNNNCTG 1 cut(s) 1112
Cfr10I RCCGGY 2 cut(s) 54, 281
CseI GACGC 2 cut(s) 112, 1282
Csp6I GTAC 2 cut(s) 563, 1258
CviAII CATG 3 cut(s) 362, 783, 1039
CviQI GTAC 2 cut(s) 563, 1258
DdeI CTNAG 8 cut(s) 29, 94, 605, 645, 843, 848, 1031, 1338
DpnI GATC 6 cut(s) 226, 553, 571, 752, 1036, 1233
DpnII GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
EaeI YGGCCR 2 cut(s) 56, 418
Eam1104I CTCTTC 4 cut(s) 444, 680, 799, 1170
EarI CTCTTC 4 cut(s) 444, 680, 799, 1170
Ecl136II GAGCTC 2 cut(s) 93, 847
Eco130I CCWWGG 1 cut(s) 1038
Eco24I GRGCYC 3 cut(s) 95, 849, 1032
Eco31I GGTCTC 2 cut(s) 470, 666
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 7 cut(s) 194, 254, 299, 347, 389, 479, 533
Eco53kI GAGCTC 2 cut(s) 93, 847
Eco57I CTGAAG 1 cut(s) 255
EcoICRI GAGCTC 2 cut(s) 93, 847
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 299
EcoRV GATATC 2 cut(s) 124, 160
EcoT14I CCWWGG 1 cut(s) 1038
EcoT22I ATGCAT 1 cut(s) 1423
EcoT38I GRGCYC 3 cut(s) 95, 849, 1032
ErhI CCWWGG 1 cut(s) 1038
FaeI CATG 3 cut(s) 365, 786, 1042
FatI CATG 3 cut(s) 361, 782, 1038
FblI GTMKAC 2 cut(s) 950, 1056
Fnu4HI GCNGC 3 cut(s) 240, 421, 996
FokI GGATG 3 cut(s) 907, 993, 1368
FriOI GRGCYC 3 cut(s) 95, 849, 1032
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 3 cut(s) 240, 421, 996
FspBI CTAG 4 cut(s) 143, 216, 939, 1386
GluI GCNGC 3 cut(s) 240, 421, 996
GsaI CCCAGC 1 cut(s) 1385
GsuI CTGGAG 3 cut(s) 333, 1134, 1170
HaeIII GGCC 8 cut(s) 39, 54, 58, 420, 436, 495, 593, 1367
HapII CCGG 3 cut(s) 55, 282, 492
HgaI GACGC 2 cut(s) 112, 1282
Hin1I GRCGYC 2 cut(s) 104, 1293
Hin1II CATG 3 cut(s) 365, 786, 1042
HpaII CCGG 3 cut(s) 55, 282, 492
HphI GGTGA 1 cut(s) 1135
Hpy166II GTNNAC 4 cut(s) 583, 721, 951, 1057
Hpy188I TCNGA 6 cut(s) 666, 838, 844, 851, 1034, 1072
Hpy8I GTNNAC 4 cut(s) 583, 721, 951, 1057
Hpy99I CGWCG 3 cut(s) 106, 1017, 1298
HpyAV CCTTC 3 cut(s) 871, 1247, 1262
HpyCH4III ACNGT 1 cut(s) 1279
HpyCH4IV ACGT 1 cut(s) 1048
HpyCH4V TGCA 3 cut(s) 716, 764, 1421
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 1395
HpyF3I CTNAG 8 cut(s) 29, 94, 605, 645, 843, 848, 1031, 1338
HpySE526I ACGT 1 cut(s) 1048
Hsp92I GRCGYC 2 cut(s) 104, 1293
Hsp92II CATG 3 cut(s) 365, 786, 1042
KroI GCCGGC 2 cut(s) 54, 281
KroNI GCCGGC 2 cut(s) 56, 283
Kzo9I GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
LguI GCTCTTC 1 cut(s) 799
LmnI GCTCC 2 cut(s) 179, 1115
Lsp1109I GCAGC 1 cut(s) 982
LweI GCATC 6 cut(s) 1230, 1346, 1400, 1408, 1423, 1430
MaeI CTAG 4 cut(s) 143, 216, 939, 1386
MaeII ACGT 1 cut(s) 1048
MaeIII GTNAC 4 cut(s) 406, 892, 1123, 1226
MalI GATC 6 cut(s) 226, 553, 571, 752, 1036, 1233
MboI GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
MboII GAAGA 9 cut(s) 204, 431, 480, 662, 667, 813, 816, 1187, 1369
MfeI CAATTG 1 cut(s) 1146
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 4 cut(s) 95, 517, 849, 1032
MluCI AATT 3 cut(s) 340, 869, 1146
MlyI GAGTC 4 cut(s) 73, 551, 926, 1120
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 1423
MroNI GCCGGC 2 cut(s) 54, 281
MroXI GAANNNNTTC 4 cut(s) 322, 526, 638, 1219
MseI TTAA 3 cut(s) 1101, 1170, 1263
MslI CAYNNNNRTG 1 cut(s) 1157
MspI CCGG 3 cut(s) 55, 282, 492
MunI CAATTG 1 cut(s) 1146
MwoI GCNNNNNNNGC 2 cut(s) 512, 1395
NaeI GCCGGC 2 cut(s) 56, 283
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
NgoMIV GCCGGC 2 cut(s) 54, 281
NheI GCTAGC 1 cut(s) 1385
NlaIII CATG 3 cut(s) 365, 786, 1042
NlaIV GGNNCC 8 cut(s) 7, 175, 238, 301, 369, 481, 514, 936
NmuCI GTSAC 2 cut(s) 1123, 1226
NsiI ATGCAT 1 cut(s) 1423
PciSI GCTCTTC 1 cut(s) 799
PdiI GCCGGC 2 cut(s) 56, 283
PdmI GAANNNNTTC 4 cut(s) 322, 526, 638, 1219
PfeI GAWTC 6 cut(s) 12, 667, 839, 986, 1142, 1162
PkrI GCNGC 3 cut(s) 241, 422, 997
Ple19I CGATCG 1 cut(s) 1234
PleI GAGTC 4 cut(s) 73, 550, 925, 1120
PpsI GAGTC 4 cut(s) 73, 550, 925, 1120
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 2 cut(s) 95, 849
Psp5II RGGWCCY 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 1381
PspN4I GGNNCC 8 cut(s) 7, 175, 238, 301, 369, 481, 514, 936
PspPPI RGGWCCY 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 1 cut(s) 1034
PvuI CGATCG 1 cut(s) 1234
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 2 cut(s) 564, 1259
RsaNI GTAC 2 cut(s) 563, 1258
RseI CAYNNNNRTG 1 cut(s) 1157
SacI GAGCTC 2 cut(s) 95, 849
SapI GCTCTTC 1 cut(s) 799
SaqAI TTAA 3 cut(s) 1101, 1170, 1263
SatI GCNGC 3 cut(s) 240, 421, 996
Sau3AI GATC 6 cut(s) 224, 551, 569, 750, 1034, 1231
SchI GAGTC 4 cut(s) 73, 551, 926, 1120
SduI GDGCHC 4 cut(s) 95, 517, 849, 1032
SfaNI GCATC 6 cut(s) 1230, 1346, 1400, 1408, 1423, 1430
SfcI CTRYAG 1 cut(s) 165
SinI GGWCC 7 cut(s) 194, 254, 299, 347, 389, 479, 533
SmiMI CAYNNNNRTG 1 cut(s) 1157
SmlI CTYRAG 2 cut(s) 914, 1391
SmoI CTYRAG 2 cut(s) 914, 1391
Sse9I AATT 3 cut(s) 340, 869, 1146
SsiI CCGC 3 cut(s) 240, 421, 1243
SspI AATATT 1 cut(s) 654
SspMI CTAG 4 cut(s) 143, 216, 939, 1386
SstI GAGCTC 2 cut(s) 95, 849
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 1 cut(s) 1279
TaiI ACGT 1 cut(s) 1051
TaqI TCGA 2 cut(s) 520, 976
TasI AATT 3 cut(s) 340, 869, 1146
TatI WGTACW 1 cut(s) 562
TauI GCSGC 2 cut(s) 242, 423
TfiI GAWTC 6 cut(s) 12, 667, 839, 986, 1142, 1162
Tru1I TTAA 3 cut(s) 1101, 1170, 1263
Tru9I TTAA 3 cut(s) 1101, 1170, 1263
TscAI CASTG 2 cut(s) 357, 766
TseFI GTSAC 2 cut(s) 1123, 1226
TseI GCWGC 1 cut(s) 995
Tsp45I GTSAC 2 cut(s) 1123, 1226
TspDTI ATGAA 4 cut(s) 572, 615, 679, 1323
TspGWI ACGGA 1 cut(s) 1119
TspRI CASTG 2 cut(s) 357, 766
VpaK11BI GGWCC 7 cut(s) 194, 254, 299, 347, 389, 479, 533
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 1 cut(s) 340
XmiI GTMKAC 2 cut(s) 950, 1056
XmnI GAANNNNTTC 4 cut(s) 322, 526, 638, 1219
XspI CTAG 4 cut(s) 143, 216, 939, 1386
Zsp2I ATGCAT 1 cut(s) 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.