Prupe.3G020900_v2.0.a1

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001653
Physical Location & Seq
Reverse (-)
1520880 .. 1526394
5515 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G020900.1

Sequence Viewer

Length: 1449 bp
ATGGGTTCCAGATTCCCATCTCATCAGCTCAGCAATGGTCTATACGTATCAGGCAGGCCTGAGCAGCCAAAAGAAAGAGCACCTACGATGGGCTCAACGGCCATGCCCTATACTGGTGGCGATATCAAGAAGTCGGGAGAGCTGGGGAAAATGTTTGATATCCCTATGGACGGGTCCAAGTCTAGGAAATCTGGACAACTAACCAGTGCCCCTTCAAGGACTGGATCATTTGGAGGTGCTGCTTCTCATTCAGGTCCAATTATGCCTAATGCTGCAGCTCGAGCTAACTATACGACATCAGGTCCTGTATCTTCTGGAGGCATGACTGGTTCTACTTCGGTAAAAAAGACTAATTCTGGGCCACTCAATAAGCATGGGGAACCATTGAAGAAGTCTTCTGGCCCTCAATCTGGTGGAGTGACACGTCAAAACTCCGGCCATATTCCACCAGCTCTTCCCACAACAGGTCTTATCACATCTGGACCCATATCTTCAGGTCCACTAAATTCCTCTGGGGCCCCTAGAAAGGTATCTGGACCTTTAGAGTCTATGGGATCAATGAAATTACAAAGTTCCTCTGTTGCTCACAATCCAGCAGTGACTACTCTTGGCCAAGATGATTATTCTTTCAGAAAGAACTTCCCAAAGACAATATTGTGGTCTGTGATACTGATATTTGTGATGGGGTTCATTGCTGGTGGTTTTATTCTTGGAGCGGTCCACAATGCCATACTCCTTGCTGTTGTGGGAATTCTTTTCGCTGCAGTTGCTGCTTTGTTCACATGGAATACTTGTTGGGGAAGAAATGCTATCGTAAATTTCATTTCTCGTTATCCTGATGCTGAGCTCAGAACTGCTAAAAATGGGCAATATGTGAAAGTCTCTGGGGTGGTTACCTGTGGTAATGTGCCCCTTGAGTCATCCTTCCGGAGAGTTCCTAGATGCGTGTACACATCTACAAGTTTATATGAGTACCGAGGATGGGATTCAAAACCAGCAAACCCTACACATCGTCGATTTACATGGGGACTTAGATCATCAGAGAGGCACATAGTGGACTTCTACATCTCTGATTTCCAGTCTGGGTTGAGAGCATTAGTTAAGACTGGCTATGGCGCAAGAGTTACTCCTTATGTTGATGAGTCTGTCATTATTGATGTTAACCCAGAAAACAAAGACATGTCTCCAGATTTTGTCAGGTGGTTGGGAGAGAGGAATCTTTCAAGTGATGACCGTACGATGCGATTGAAAGAAGGGTACATCAAGGAAGGTAGCACAGTTAGTGTGATGGGAGTTGTCCAGAGAAATGACAATGTGCTTATGATTGTCCCTCCGCCTGAGCCTTTGACAACTGGATGCCAATGGAGCAATTGTATTTTTCCGGCTAGCCTTGAGGGGGTTGTTTTGAGATGTGAAGACTCATCAAAAAATGATGTCATACCCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

483

Amino Acids

51.69

Weight (kDa)

9.59

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 716
AccIII TCCGGA 1 cut(s) 927
AciI CCGC 2 cut(s) 716, 1334
AclWI GGATC 2 cut(s) 232, 562
AcoI YGGCCR 3 cut(s) 99, 436, 610
AcsI RAATTY 3 cut(s) 505, 750, 817
AcuI CTGAAG 1 cut(s) 477
AdeI CACNNNGTG 1 cut(s) 1054
AfaI GTAC 4 cut(s) 950, 974, 1237, 1259
AflIII ACRYGT 2 cut(s) 422, 1179
AgsI TTSAA 5 cut(s) 216, 388, 990, 1224, 1249
AhdI GACNNNNNGTC 1 cut(s) 300
AjiI CACGTC 1 cut(s) 425
AluBI AGCT 6 cut(s) 28, 142, 278, 284, 452, 847
AluI AGCT 6 cut(s) 28, 142, 278, 284, 452, 847
Alw21I GWGCWC 2 cut(s) 82, 849
Alw26I GTCTC 2 cut(s) 886, 1188
AlwI GGATC 2 cut(s) 232, 562
AlwNI CAGNNNCTG 2 cut(s) 305, 770
Ama87I CYCGRG 1 cut(s) 279
Aor13HI TCCGGA 1 cut(s) 927
AoxI GGCC 7 cut(s) 56, 99, 359, 400, 436, 516, 610
ApaI GGGCCC 1 cut(s) 520
ApeKI GCWGC 6 cut(s) 64, 239, 272, 275, 761, 770
ApoI RAATTY 3 cut(s) 505, 750, 817
AspLEI GCGC 1 cut(s) 1118
AsuNHI GCTAGC 1 cut(s) 1385
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 7 cut(s) 174, 254, 302, 482, 497, 536, 718
BaeGI GKGCMC 3 cut(s) 211, 520, 912
BalI TGGCCA 1 cut(s) 612
BanII GRGCYC 3 cut(s) 95, 520, 849
BbsI GAAGAC 2 cut(s) 387, 1422
Bbv12I GWGCWC 2 cut(s) 82, 849
BbvI GCAGC 6 cut(s) 76, 226, 259, 287, 748, 757
BccI CCATC 5 cut(s) 25, 82, 676, 975, 1282
BceAI ACGGC 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 886, 1188
BfaI CTAG 4 cut(s) 183, 522, 939, 1386
BfmI CTRYAG 2 cut(s) 273, 762
BisI GCNGC 6 cut(s) 65, 240, 273, 276, 762, 771
BlpI GCTNAGC 2 cut(s) 29, 843
BlsI GCNGC 6 cut(s) 66, 241, 274, 277, 763, 772
Bme18I GGWCC 7 cut(s) 174, 254, 302, 482, 497, 536, 718
BmeRI GACNNNNNGTC 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 425
BmiI GGNNCC 7 cut(s) 7, 175, 381, 484, 517, 518, 519
BmsI GCATC 4 cut(s) 829, 932, 1230, 1346
BmtI GCTAGC 1 cut(s) 1389
BpiI GAAGAC 2 cut(s) 387, 1422
BpmI CTGGAG 2 cut(s) 336, 1170
Bpu10I CCTNAGC 2 cut(s) 60, 1338
Bpu1102I GCTNAGC 2 cut(s) 29, 843
BpuEI CTTGAG 2 cut(s) 935, 1412
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 976
BsaWI WCCGGW 1 cut(s) 927
BsaXI ACNNNNNCTCC 2 cut(s) 1357, 1387
Bse1I ACTGG 7 cut(s) 118, 204, 226, 331, 1078, 1111, 1357
Bse3DI GCAATG 2 cut(s) 40, 690
Bse8I GATNNNNATC 1 cut(s) 16
BseAI TCCGGA 1 cut(s) 927
BseDI CCNNGG 1 cut(s) 976
BseGI GGATG 3 cut(s) 920, 986, 1361
BseJI GATNNNNATC 1 cut(s) 16
BseMI GCAATG 2 cut(s) 40, 690
BseMII CTCAG 5 cut(s) 43, 51, 834, 862, 1329
BseNI ACTGG 7 cut(s) 118, 204, 226, 331, 1078, 1111, 1357
BseSI GKGCMC 3 cut(s) 211, 520, 912
BseXI GCAGC 6 cut(s) 76, 226, 259, 287, 748, 757
BseYI CCCAGC 1 cut(s) 142
BshFI GGCC 7 cut(s) 58, 101, 361, 402, 438, 518, 612
BsiHKAI GWGCWC 2 cut(s) 82, 849
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 3 cut(s) 435, 928, 1382
BsiWI CGTACG 1 cut(s) 1235
BslFI GGGAC 2 cut(s) 1041, 1313
BsmAI GTCTC 2 cut(s) 886, 1188
BsmFI GGGAC 2 cut(s) 1041, 1313
BsnI GGCC 7 cut(s) 58, 101, 361, 402, 438, 518, 612
BsoBI CYCGRG 1 cut(s) 279
Bsp120I GGGCCC 1 cut(s) 516
Bsp1286I GDGCHC 6 cut(s) 82, 95, 211, 520, 849, 912
Bsp13I TCCGGA 1 cut(s) 927
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 3 cut(s) 224, 554, 1034
Bsp1720I GCTNAGC 2 cut(s) 29, 843
BspACI CCGC 2 cut(s) 716, 1334
BspANI GGCC 7 cut(s) 58, 101, 361, 402, 438, 518, 612
BspCNI CTCAG 5 cut(s) 42, 52, 835, 861, 1330
BspEI TCCGGA 1 cut(s) 927
BspLI GGNNCC 7 cut(s) 7, 175, 381, 484, 517, 518, 519
BspMAI CTGCAG 2 cut(s) 277, 766
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 2 cut(s) 232, 562
BspQI GCTCTTC 1 cut(s) 459
BsrBI CCGCTC 1 cut(s) 716
BsrDI GCAATG 2 cut(s) 40, 690
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 7 cut(s) 118, 204, 226, 331, 1078, 1111, 1357
BssECI CCNNGG 1 cut(s) 976
BssMI GATC 3 cut(s) 224, 554, 1034
Bst4CI ACNGT 2 cut(s) 1235, 1279
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 948
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 56, 1387
BstDEI CTNAG 6 cut(s) 29, 60, 843, 848, 1031, 1338
BstEII GGTNACC 1 cut(s) 892
BstF5I GGATG 3 cut(s) 920, 986, 1361
BstHHI GCGC 1 cut(s) 1118
BstKTI GATC 3 cut(s) 227, 557, 1037
BstMAI GTCTC 2 cut(s) 886, 1188
BstMBI GATC 3 cut(s) 224, 554, 1034
BstMWI GCNNNNNNNGC 5 cut(s) 64, 281, 767, 770, 1365
BstNSI RCATGY 1 cut(s) 1183
BstPI GGTNACC 1 cut(s) 892
BstSFI CTRYAG 2 cut(s) 273, 762
BstSLI GKGCMC 3 cut(s) 211, 520, 912
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 6 cut(s) 76, 226, 259, 287, 748, 757
BstV2I GAAGAC 2 cut(s) 387, 1422
BsuRI GGCC 7 cut(s) 58, 101, 361, 402, 438, 518, 612
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 3 cut(s) 920, 986, 1361
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 2 cut(s) 211, 603
Cac8I GCNNGC 2 cut(s) 56, 1387
CaiI CAGNNNCTG 2 cut(s) 305, 770
CfoI GCGC 1 cut(s) 1118
Csp6I GTAC 4 cut(s) 949, 973, 1236, 1258
CviAII CATG 6 cut(s) 103, 322, 374, 783, 1023, 1180
CviQI GTAC 4 cut(s) 949, 973, 1236, 1258
DdeI CTNAG 6 cut(s) 29, 60, 843, 848, 1031, 1338
DpnI GATC 3 cut(s) 226, 556, 1036
DpnII GATC 3 cut(s) 224, 554, 1034
DraIII CACNNNGTG 1 cut(s) 1054
DriI GACNNNNNGTC 1 cut(s) 300
EaeI YGGCCR 3 cut(s) 99, 436, 610
Eam1104I CTCTTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 1323
Ecl136II GAGCTC 1 cut(s) 847
Eco105I TACGTA 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 58
Eco24I GRGCYC 3 cut(s) 95, 520, 849
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 7 cut(s) 174, 254, 302, 482, 497, 536, 718
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 279
Eco91I GGTNACC 1 cut(s) 892
EcoICRI GAGCTC 1 cut(s) 847
EcoO109I RGGNCCY 3 cut(s) 302, 516, 517
EcoO65I GGTNACC 1 cut(s) 892
EcoRI GAATTC 1 cut(s) 750
EcoRV GATATC 2 cut(s) 124, 160
EcoT38I GRGCYC 3 cut(s) 95, 520, 849
FaeI CATG 6 cut(s) 106, 325, 377, 786, 1026, 1183
FaqI GGGAC 2 cut(s) 1041, 1313
FatI CATG 6 cut(s) 102, 321, 373, 782, 1022, 1179
Fnu4HI GCNGC 6 cut(s) 65, 240, 273, 276, 762, 771
FokI GGATG 3 cut(s) 907, 993, 1368
FriOI GRGCYC 3 cut(s) 95, 520, 849
Fsp4HI GCNGC 6 cut(s) 65, 240, 273, 276, 762, 771
FspBI CTAG 4 cut(s) 183, 522, 939, 1386
GlaI GCGC 1 cut(s) 1117
GluI GCNGC 6 cut(s) 65, 240, 273, 276, 762, 771
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 2 cut(s) 336, 1170
HaeIII GGCC 7 cut(s) 58, 101, 361, 402, 438, 518, 612
HapII CCGG 3 cut(s) 435, 928, 1382
HhaI GCGC 1 cut(s) 1118
Hin1II CATG 6 cut(s) 106, 325, 377, 786, 1026, 1183
Hin6I GCGC 1 cut(s) 1116
HinP1I GCGC 1 cut(s) 1116
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HinfI GANTC 7 cut(s) 12, 545, 917, 986, 1142, 1216, 1418
HpaI GTTAAC 1 cut(s) 1162
HpaII CCGG 3 cut(s) 435, 928, 1382
Hpy166II GTNNAC 7 cut(s) 500, 721, 780, 949, 951, 1057, 1162
Hpy188I TCNGA 4 cut(s) 632, 851, 1042, 1072
Hpy8I GTNNAC 7 cut(s) 500, 721, 780, 949, 951, 1057, 1162
Hpy99I CGWCG 1 cut(s) 1017
HpyAV CCTTC 4 cut(s) 222, 934, 1247, 1262
HpyCH4III ACNGT 2 cut(s) 1235, 1279
HpyCH4IV ACGT 2 cut(s) 45, 424
HpyCH4V TGCA 2 cut(s) 275, 764
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 281, 767, 770, 1365
HpyF3I CTNAG 6 cut(s) 29, 60, 843, 848, 1031, 1338
HpySE526I ACGT 2 cut(s) 45, 424
Hsp92II CATG 6 cut(s) 106, 325, 377, 786, 1026, 1183
HspAI GCGC 1 cut(s) 1116
Kpn2I TCCGGA 1 cut(s) 927
KspAI GTTAAC 1 cut(s) 1162
Kzo9I GATC 3 cut(s) 224, 554, 1034
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 713, 1365
Lsp1109I GCAGC 6 cut(s) 76, 226, 259, 287, 748, 757
LweI GCATC 4 cut(s) 829, 932, 1230, 1346
MaeI CTAG 4 cut(s) 183, 522, 939, 1386
MaeII ACGT 2 cut(s) 45, 424
MaeIII GTNAC 4 cut(s) 418, 598, 892, 1123
MalI GATC 3 cut(s) 226, 556, 1036
MbiI CCGCTC 1 cut(s) 716
MboI GATC 3 cut(s) 224, 554, 1034
MboII GAAGA 7 cut(s) 303, 387, 400, 446, 483, 813, 1427
MfeI CAATTG 1 cut(s) 1369
MhlI GDGCHC 6 cut(s) 82, 95, 211, 520, 849, 912
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 7 cut(s) 258, 352, 505, 563, 750, 817, 1369
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 4 cut(s) 554, 926, 1151, 1412
Mox20I TGGCCA 1 cut(s) 612
MroI TCCGGA 1 cut(s) 927
MscI TGGCCA 1 cut(s) 612
MseI TTAA 2 cut(s) 1101, 1161
Msp20I TGGCCA 1 cut(s) 612
MspI CCGG 3 cut(s) 435, 928, 1382
MunI CAATTG 1 cut(s) 1369
MwoI GCNNNNNNNGC 5 cut(s) 64, 281, 767, 770, 1365
NdeII GATC 3 cut(s) 224, 554, 1034
NheI GCTAGC 1 cut(s) 1385
NlaIII CATG 6 cut(s) 106, 325, 377, 786, 1026, 1183
NlaIV GGNNCC 7 cut(s) 7, 175, 381, 484, 517, 518, 519
NmuCI GTSAC 2 cut(s) 418, 598
NspI RCATGY 1 cut(s) 1183
PaeR7I CTCGAG 1 cut(s) 279
PceI AGGCCT 1 cut(s) 58
PciI ACATGT 1 cut(s) 1179
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 3 cut(s) 12, 986, 1216
Pfl23II CGTACG 1 cut(s) 1235
PkrI GCNGC 6 cut(s) 66, 241, 274, 277, 763, 772
PleI GAGTC 4 cut(s) 553, 925, 1150, 1412
PpsI GAGTC 4 cut(s) 553, 925, 1150, 1412
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 302
PscI ACATGT 1 cut(s) 1179
Psp124BI GAGCTC 1 cut(s) 849
Psp5II RGGWCCY 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 892
PspFI CCCAGC 1 cut(s) 142
PspLI CGTACG 1 cut(s) 1235
PspN4I GGNNCC 7 cut(s) 7, 175, 381, 484, 517, 518, 519
PspOMI GGGCCC 1 cut(s) 516
PspPPI RGGWCCY 1 cut(s) 302
PspXI VCTCGAGB 1 cut(s) 279
PstI CTGCAG 2 cut(s) 277, 766
PstNI CAGNNNCTG 2 cut(s) 305, 770
RsaI GTAC 4 cut(s) 950, 974, 1237, 1259
RsaNI GTAC 4 cut(s) 949, 973, 1236, 1258
SacI GAGCTC 1 cut(s) 849
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 1101, 1161
SatI GCNGC 6 cut(s) 65, 240, 273, 276, 762, 771
Sau3AI GATC 3 cut(s) 224, 554, 1034
SchI GAGTC 4 cut(s) 554, 926, 1151, 1412
SduI GDGCHC 6 cut(s) 82, 95, 211, 520, 849, 912
SfaNI GCATC 4 cut(s) 829, 932, 1230, 1346
SfcI CTRYAG 2 cut(s) 273, 762
Sfr274I CTCGAG 1 cut(s) 279
SinI GGWCC 7 cut(s) 174, 254, 302, 482, 497, 536, 718
SlaI CTCGAG 1 cut(s) 279
SmlI CTYRAG 3 cut(s) 279, 914, 1391
SmoI CTYRAG 3 cut(s) 279, 914, 1391
SnaBI TACGTA 1 cut(s) 46
Sse9I AATT 7 cut(s) 258, 352, 505, 563, 750, 817, 1369
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 2 cut(s) 716, 1334
SspI AATATT 1 cut(s) 654
SspMI CTAG 4 cut(s) 183, 522, 939, 1386
SstI GAGCTC 1 cut(s) 849
StuI AGGCCT 1 cut(s) 58
TaaI ACNGT 2 cut(s) 1235, 1279
TaiI ACGT 2 cut(s) 48, 427
TaqI TCGA 2 cut(s) 280, 1015
TasI AATT 7 cut(s) 258, 352, 505, 563, 750, 817, 1369
TatI WGTACW 1 cut(s) 948
TfiI GAWTC 3 cut(s) 12, 986, 1216
Tru1I TTAA 2 cut(s) 1101, 1161
Tru9I TTAA 2 cut(s) 1101, 1161
TscAI CASTG 2 cut(s) 211, 603
TseFI GTSAC 2 cut(s) 418, 598
TseI GCWGC 6 cut(s) 64, 239, 272, 275, 761, 770
Tsp45I GTSAC 2 cut(s) 418, 598
TspDTI ATGAA 3 cut(s) 575, 679, 811
TspRI CASTG 2 cut(s) 211, 603
VpaK11BI GGWCC 7 cut(s) 174, 254, 302, 482, 497, 536, 718
XapI RAATTY 3 cut(s) 505, 750, 817
XceI RCATGY 1 cut(s) 1183
XhoI CTCGAG 1 cut(s) 279
XspI CTAG 4 cut(s) 183, 522, 939, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.