Rorug02G0413800

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001653
Physical Location & Seq
Reverse (-)
52999150 .. 53000476
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0413800.1

Sequence Viewer

Length: 786 bp
ATGAAAACATTCCCGGTACTACCACTCTTCTTCCTAGCCACACTGGTGGCAACAAACCAAGCTTCAGACAGTGCTGTATCCCACCAGATTCATCTCCTCCGGCCAAAAACCGGTTCCCATGGGAGCCACGTCCCCCGCGTTTCGTGCCTAAGCTGGAGACTCGCCGTCGAAACCCACAATATTATTGGGTGGAAGACTATTCCAGCGGCTTGTGAAGGCTATGTAGGGCACTACATGCTTGGCCACCAGTACAGAAAAGACTCGAAAGCTATTACTTATGAGGCGTATCTCTACGCTAAGAGCCTGAATCTTACCAAGGATGGCAAGAACTTGTGGGTCTTTGACGTAGATGAGACTACTCTCTCCAACCTACCTTATTATGCTCGCCATGGATTCGGGGTGGAGGTATTTAATCCCACTTTATTCAACGAATGGGTCTCGAAAAGCACAGCCCCAGCCTTACCTGAGAGTCTTAAGTTGTACAAGAAATTATTGGCTCTTGGAATTAAGGTTGCATTCTTAACAGGAAGACCAGAATCTTATAGAAAGGCCACAGAAACCAATCTAAAAAATGTTGGATATCACACTTGGGAAACGGCTATACTAAAAGATTCATCTTATGCAGGTAAGACATCACTCTTTTATAAGTCATCTGAGAGAGAGAAGCTTGAGAAGAAAGGATACAGAATAATTGGGAACATGGGCGATCAATGGACTGATCTCTTGGGGACAAATGCTGGCAACCGGACCTTCAAGGTGCCGGATCCAATGTATTACATTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.47

Weight (kDa)

9.39

Isoelectric Point (pI)

30.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 45 - 260 3.6e-84 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
Acc36I ACCTGC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 757
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 136, 206
AclWI GGATC 2 cut(s) 758, 771
AcoI YGGCCR 2 cut(s) 101, 241
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 18, 251, 482
AfiI CCNNNNNNNGG 1 cut(s) 110
AflII CTTAAG 1 cut(s) 473
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 2 cut(s) 427, 754
AhdI GACNNNNNGTC 1 cut(s) 164
AjiI CACGTC 1 cut(s) 130
AjuI GAANNNNNNNTTGG 2 cut(s) 97, 129
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 4 cut(s) 62, 153, 269, 667
AluI AGCT 4 cut(s) 62, 153, 269, 667
Alw26I GTCTC 3 cut(s) 151, 347, 442
AlwI GGATC 2 cut(s) 758, 771
AoxI GGCC 3 cut(s) 101, 241, 549
AsiGI ACCGGT 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 747
AsuC2I CCSGG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 747
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 243
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 757
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BbsI GAAGAC 2 cut(s) 200, 535
BccI CCATC 1 cut(s) 314
BceAI ACGGC 2 cut(s) 149, 612
BciVI GTATCC 2 cut(s) 88, 674
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 3 cut(s) 151, 347, 442
BfaI CTAG 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 614
BfuI GTATCC 2 cut(s) 88, 674
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 747
BmeRI GACNNNNNGTC 1 cut(s) 164
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 4 cut(s) 115, 125, 759, 765
BmrFI CCNGG 1 cut(s) 14
BpiI GAAGAC 2 cut(s) 200, 535
BpmI CTGGAG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 442
BsaJI CCNNGG 3 cut(s) 118, 315, 388
BsaWI WCCGGW 2 cut(s) 110, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 48, 247
BseDI CCNNGG 3 cut(s) 118, 315, 388
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 2 cut(s) 456, 645
BseNI ACTGG 2 cut(s) 48, 247
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 231
BseYI CCCAGC 1 cut(s) 454
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 103, 243, 551
BshNI GGYRCC 1 cut(s) 757
BshTI ACCGGT 1 cut(s) 110
BsiSI CCGG 5 cut(s) 14, 100, 111, 745, 761
BslFI GGGAC 2 cut(s) 116, 742
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 3 cut(s) 151, 347, 442
BsmFI GGGAC 2 cut(s) 116, 742
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 3 cut(s) 103, 243, 551
Bso31I GGTCTC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 231
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 3 cut(s) 706, 718, 763
Bsp19I CCATGG 2 cut(s) 118, 388
BspACI CCGC 2 cut(s) 136, 206
BspANI GGCC 3 cut(s) 103, 243, 551
BspCNI CTCAG 2 cut(s) 457, 646
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 115, 125, 759, 765
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 2 cut(s) 758, 771
BspT107I GGYRCC 1 cut(s) 757
BspTI CTTAAG 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 2 cut(s) 48, 247
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 3 cut(s) 118, 315, 388
BssMI GATC 3 cut(s) 706, 718, 763
BssT1I CCWWGG 3 cut(s) 118, 315, 388
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 473
BstAPI GCANNNNNTGC 1 cut(s) 235
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 385, 739
BstDEI CTNAG 4 cut(s) 149, 297, 465, 654
BstDSI CCRYGG 2 cut(s) 118, 388
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 3 cut(s) 709, 721, 766
BstMAI GTCTC 3 cut(s) 151, 347, 442
BstMBI GATC 3 cut(s) 706, 718, 763
BstMWI GCNNNNNNNGC 2 cut(s) 144, 235
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 1 cut(s) 138
BstV2I GAAGAC 2 cut(s) 200, 535
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 763
BsuI GTATCC 2 cut(s) 88, 674
BsuRI GGCC 3 cut(s) 103, 243, 551
BtgI CCRYGG 2 cut(s) 118, 388
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 41, 76
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 2 cut(s) 385, 739
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 747
Csp6I GTAC 3 cut(s) 17, 250, 481
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 4 cut(s) 119, 235, 389, 700
CviQI GTAC 3 cut(s) 17, 250, 481
DdeI CTNAG 4 cut(s) 149, 297, 465, 654
DpnI GATC 3 cut(s) 708, 720, 765
DpnII GATC 3 cut(s) 706, 718, 763
DriI GACNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 101, 241
Eam1104I CTCTTC 1 cut(s) 32
Eam1105I GACNNNNNGTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 3 cut(s) 118, 315, 388
Eco31I GGTCTC 1 cut(s) 442
Eco32I GATATC 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 747
Eco57I CTGAAG 1 cut(s) 48
EcoRV GATATC 1 cut(s) 581
EcoT14I CCWWGG 3 cut(s) 118, 315, 388
ErhI CCWWGG 3 cut(s) 118, 315, 388
FaeI CATG 4 cut(s) 122, 238, 392, 703
FaqI GGGAC 2 cut(s) 116, 742
FatI CATG 4 cut(s) 118, 234, 388, 699
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 1 cut(s) 35
GluI GCNGC 1 cut(s) 207
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 3 cut(s) 103, 243, 551
HapII CCGG 5 cut(s) 14, 100, 111, 745, 761
Hin1II CATG 4 cut(s) 122, 238, 392, 703
HindIII AAGCTT 2 cut(s) 60, 665
HinfI GANTC 8 cut(s) 88, 159, 260, 307, 393, 469, 536, 611
HpaII CCGG 5 cut(s) 14, 100, 111, 745, 761
Hpy188I TCNGA 2 cut(s) 67, 655
Hpy188III TCNNGA 1 cut(s) 439
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 209, 760
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 2 cut(s) 129, 345
HpyCH4V TGCA 2 cut(s) 515, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 235
HpyF3I CTNAG 4 cut(s) 149, 297, 465, 654
HpySE526I ACGT 2 cut(s) 129, 345
Hsp92II CATG 4 cut(s) 122, 238, 392, 703
Kzo9I GATC 3 cut(s) 706, 718, 763
LmnI GCTCC 1 cut(s) 123
MaeI CTAG 1 cut(s) 35
MaeII ACGT 2 cut(s) 129, 345
MalI GATC 3 cut(s) 708, 720, 765
MboI GATC 3 cut(s) 706, 718, 763
MboII GAAGA 5 cut(s) 19, 22, 205, 540, 685
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 243
MluCI AATT 3 cut(s) 488, 504, 690
MluNI TGGCCA 1 cut(s) 243
MlyI GAGTC 3 cut(s) 153, 254, 478
MmeI TCCRAC 2 cut(s) 390, 556
MnlI CCTC 3 cut(s) 107, 274, 397
Mox20I TGGCCA 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 243
MseI TTAA 4 cut(s) 411, 474, 507, 521
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 206
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 5 cut(s) 14, 100, 111, 745, 761
MspR9I CCNGG 1 cut(s) 14
Mva1269I GAATGC 1 cut(s) 515
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 144, 235
NciI CCSGG 1 cut(s) 14
NcoI CCATGG 2 cut(s) 118, 388
NdeII GATC 3 cut(s) 706, 718, 763
NlaIII CATG 4 cut(s) 122, 238, 392, 703
NlaIV GGNNCC 4 cut(s) 115, 125, 759, 765
NspI RCATGY 1 cut(s) 238
OliI CACNNNNGTG 1 cut(s) 44
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 5 cut(s) 88, 307, 393, 536, 611
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 1 cut(s) 208
PleI GAGTC 3 cut(s) 153, 254, 477
PpsI GAGTC 3 cut(s) 153, 254, 477
PsiI TTATAA 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 454
PspN4I GGNNCC 4 cut(s) 115, 125, 759, 765
PspPI GGNCC 1 cut(s) 747
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 3 cut(s) 18, 251, 482
RsaNI GTAC 3 cut(s) 17, 250, 481
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 4 cut(s) 411, 474, 507, 521
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 3 cut(s) 706, 718, 763
Sau96I GGNCC 1 cut(s) 747
SchI GAGTC 3 cut(s) 153, 254, 478
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 231
SinI GGWCC 1 cut(s) 747
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 473, 668
SmoI CTYRAG 2 cut(s) 473, 668
Sse9I AATT 3 cut(s) 488, 504, 690
SsiI CCGC 2 cut(s) 136, 206
SspI AATATT 1 cut(s) 181
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 3 cut(s) 118, 315, 388
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 2 cut(s) 132, 348
TaqI TCGA 3 cut(s) 168, 263, 440
TasI AATT 3 cut(s) 488, 504, 690
TatI WGTACW 2 cut(s) 249, 480
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 5 cut(s) 88, 307, 393, 536, 611
Tru1I TTAA 4 cut(s) 411, 474, 507, 521
Tru9I TTAA 4 cut(s) 411, 474, 507, 521
TscAI CASTG 2 cut(s) 48, 76
TspDTI ATGAA 3 cut(s) 17, 80, 603
TspRI CASTG 2 cut(s) 48, 76
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 747
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.