MD17G1164300.v1.1

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001653
Physical Location & Seq
Forward (+)
16248695 .. 16253477
4783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1164300.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGGTTCCAGATTCAATTCTCATCAACTCAGTAATGGCCTTTACGTATCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCCATGCCCTATACTGGTGGAGATATCAAGAAGTCAGGAGAACTAGGGAAGATGTTTGATATCCCTACGGATGCCTCTAAGTCTAGAAAATCTGGACCTATAACTGGTCCTCCTGCAAGGACTGGATCTTTTGGAGGTGCTGCTTCACATTCAGGACCAATCCCTAATGCTACAGTTCGTGCTGGCCATACCACATCAGGTCCTGTATCATCTGGAGGCATGCCTGGTTCTGCTTCCTTAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCTCTAAAGAAGTCGTCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCAGGACCTCTTCCTCCTGTTCTCCCTGCAACAGGTCTCATTACATCTGGACCCATTTCTTCTGGTCCACTAAATTCTTCTGGTGCCCCTCGGAAGGTATCTGGTCCTTTGGAGTCAATGGGTTCAATGAAAGTACATGGTTCCTCCATTGTTCACAATCAGGCCATTACTACTCTTAGCCAAGATGACGAATTTTCGTTTAGGAAGAACTTCCCAAAGCTAATTATGTGGTCTTTGATTCTTCTTTTCGTGATGGGGTTCATTGCTGGTGGTTTTATTCTAGGTGCAGTCCACAATGCAATTCTCCTCATCGTGGTTGTGATCCTTTTCGGTGCAGTTGCTACACTATTTACATGGAATACTTATTGGGGAAGACGAGCTATCATAGGTTATATTTCTAGTTATTCAGATTCCGAGCTTAGGACTGCAAAGAACGGGCAATTTGTGAAGGTGTCAGGGGTGGTCACTTGTGGCAATCTGCCCCTTGAGTCGTCCTTTCAAAGGATTCCAAGATGTGTGTATACATCTACAAGTTTATATGAGTATCGAGGATGGGGTTCAAAAGCTGCCCACCCTACACATCGTCGCTTTACTTGGGGGCTCAGGTCGATAGAAAGGCGTGTGGTAGATTTTTACATCTCTGATTTCCAATCTGGGTTAAGAGCATTGGTAAAGACTGGCTGTGGGGCAAGGGTGACTCCTTATGTTGATGATTCAATTGTCATTGATGTGAATCCGGAACATGAAGAGCTGTCTCCAGAGTTTATTAGATGGCTGGGAGAGAGGAAACTTTCAAGCAATGATCGCATTATGCGGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTTAGTGTAATGGGAGTTGTCCAGAGAAATGACAATGTGCTGATGATTGTGCCTCCACCTGAGCCGGTCACGACAGGATGCCAGTGGGCCAAATGTATCTTTCCAGCCAGCCTCGAGGGTATTGTGTTGAGATGTGATGATGCATCAAAGAATGATGTCATCCCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

486

Amino Acids

51.99

Weight (kDa)

9.66

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1400
AccB1I GGYRCC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 959
AccIII TCCGGA 1 cut(s) 1174
AciI CCGC 2 cut(s) 430, 1252
AclWI GGATC 2 cut(s) 232, 754
AcoI YGGCCR 3 cut(s) 56, 283, 427
AcsI RAATTY 3 cut(s) 349, 511, 629
AfaI GTAC 2 cut(s) 573, 1268
AfiI CCNNNNNNNGG 8 cut(s) 113, 203, 407, 470, 532, 751, 858, 939
AgsI TTSAA 7 cut(s) 16, 564, 938, 999, 1155, 1233, 1258
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 6 cut(s) 93, 658, 818, 856, 1004, 1189
AluI AGCT 6 cut(s) 93, 658, 818, 856, 1004, 1189
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 479, 1197
AlwI GGATC 2 cut(s) 232, 754
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 1400
Aor13HI TCCGGA 1 cut(s) 1174
AoxI GGCC 7 cut(s) 37, 52, 56, 283, 427, 600, 1374
ApeKI GCWGC 2 cut(s) 239, 1004
ApoI RAATTY 3 cut(s) 349, 511, 629
Asp700I GAANNNNTTC 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 1144
AvaI CYCGRG 1 cut(s) 1400
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 1 cut(s) 285
BanI GGYRCC 1 cut(s) 521
BanII GRGCYC 2 cut(s) 95, 1041
BbsI GAAGAC 1 cut(s) 817
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 2 cut(s) 226, 991
BccI CCATC 3 cut(s) 685, 984, 1203
BciT130I CCWGG 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 479, 1197
BfaI CTAG 4 cut(s) 143, 183, 719, 837
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 3 cut(s) 240, 430, 1005
BlsI GCNGC 3 cut(s) 241, 431, 1006
Bme1390I CCNGG 1 cut(s) 324
BmeT110I CYCGRG 1 cut(s) 1400
BmiI GGNNCC 5 cut(s) 7, 378, 490, 523, 580
BmrFI CCNGG 1 cut(s) 324
BmrI ACTGGG 1 cut(s) 1445
BmsI GCATC 4 cut(s) 160, 1355, 1417, 1439
BmuI ACTGGG 1 cut(s) 1445
BpiI GAAGAC 1 cut(s) 817
BpmI CTGGAG 2 cut(s) 333, 1179
Bpu10I CCTNAGC 3 cut(s) 857, 1040, 1347
BpuEI CTTGAG 1 cut(s) 944
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 1170
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 527
BsaWI WCCGGW 1 cut(s) 1174
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 8 cut(s) 113, 203, 407, 470, 532, 751, 858, 939
Bse118I RCCGGY 2 cut(s) 54, 1351
Bse1I ACTGG 7 cut(s) 118, 208, 226, 430, 1120, 1369, 1451
Bse3DI GCAATG 2 cut(s) 699, 1243
Bse8I GATNNNNATC 1 cut(s) 1170
BseAI TCCGGA 1 cut(s) 1174
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 4 cut(s) 175, 995, 1370, 1446
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 8 cut(s) 113, 203, 407, 470, 532, 751, 858, 939
BseMI GCAATG 2 cut(s) 699, 1243
BseMII CTCAG 5 cut(s) 43, 108, 453, 1054, 1338
BseNI ACTGG 7 cut(s) 118, 208, 226, 430, 1120, 1369, 1451
BseRI GAGGAG 1 cut(s) 734
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 2 cut(s) 226, 991
BseYI CCCAGC 1 cut(s) 1213
BsgI GTGCAG 2 cut(s) 744, 792
BshFI GGCC 7 cut(s) 39, 54, 58, 285, 429, 602, 1376
BshNI GGYRCC 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 95
BsiHKCI CYCGRG 1 cut(s) 1400
BsiSI CCGG 3 cut(s) 55, 1175, 1352
BslI CCNNNNNNNGG 8 cut(s) 113, 203, 407, 470, 532, 751, 858, 939
BsmAI GTCTC 2 cut(s) 479, 1197
BsnI GGCC 7 cut(s) 39, 54, 58, 285, 429, 602, 1376
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 1400
Bsp1286I GDGCHC 3 cut(s) 95, 526, 1041
Bsp13I TCCGGA 1 cut(s) 1174
Bsp143I GATC 3 cut(s) 224, 759, 1240
BspACI CCGC 2 cut(s) 430, 1252
BspANI GGCC 7 cut(s) 39, 54, 58, 285, 429, 602, 1376
BspCNI CTCAG 5 cut(s) 42, 107, 452, 1053, 1339
BspEI TCCGGA 1 cut(s) 1174
BspLI GGNNCC 5 cut(s) 7, 378, 490, 523, 580
BspPI GGATC 2 cut(s) 232, 754
BspQI GCTCTTC 1 cut(s) 1179
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 699, 1243
BsrFI RCCGGY 2 cut(s) 54, 1351
BsrI ACTGG 7 cut(s) 118, 208, 226, 430, 1120, 1369, 1451
BssAI RCCGGY 2 cut(s) 54, 1351
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 3 cut(s) 224, 759, 1240
BssNAI GTATAC 1 cut(s) 960
Bst1107I GTATAC 1 cut(s) 960
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 274, 1288
Bst6I CTCTTC 2 cut(s) 453, 1179
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 56, 283, 320, 1396
BstDEI CTNAG 8 cut(s) 29, 94, 177, 439, 614, 857, 1040, 1347
BstENI CCTNNNNNAGG 2 cut(s) 468, 937
BstF5I GGATG 4 cut(s) 175, 995, 1370, 1446
BstKTI GATC 3 cut(s) 227, 762, 1243
BstMAI GTCTC 2 cut(s) 479, 1197
BstMBI GATC 3 cut(s) 224, 759, 1240
BstMWI GCNNNNNNNGC 1 cut(s) 1242
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 526
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 226, 991
BstV2I GAAGAC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 960
BsuRI GGCC 7 cut(s) 39, 54, 58, 285, 429, 602, 1376
BtsCI GGATG 4 cut(s) 175, 995, 1370, 1446
BtsIMutI CAGTG 2 cut(s) 359, 1376
Cac8I GCNNGC 4 cut(s) 56, 283, 320, 1396
CaiI CAGNNNCTG 1 cut(s) 302
Cfr10I RCCGGY 2 cut(s) 54, 1351
Csp6I GTAC 2 cut(s) 572, 1267
CviAII CATG 6 cut(s) 103, 319, 371, 575, 792, 1181
CviQI GTAC 2 cut(s) 572, 1267
DdeI CTNAG 8 cut(s) 29, 94, 177, 439, 614, 857, 1040, 1347
DpnI GATC 3 cut(s) 226, 761, 1242
DpnII GATC 3 cut(s) 224, 759, 1240
EaeI YGGCCR 3 cut(s) 56, 283, 427
Eam1104I CTCTTC 2 cut(s) 453, 1179
EarI CTCTTC 2 cut(s) 453, 1179
Ecl136II GAGCTC 1 cut(s) 93
Eco105I TACGTA 1 cut(s) 46
Eco24I GRGCYC 2 cut(s) 95, 1041
Eco31I GGTCTC 1 cut(s) 479
Eco32I GATATC 2 cut(s) 124, 160
Eco53kI GAGCTC 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 1400
EcoICRI GAGCTC 1 cut(s) 93
EcoNI CCTNNNNNAGG 2 cut(s) 468, 937
EcoO109I RGGNCCY 2 cut(s) 299, 443
EcoRII CCWGG 1 cut(s) 322
EcoRV GATATC 2 cut(s) 124, 160
EcoT22I ATGCAT 1 cut(s) 1432
EcoT38I GRGCYC 2 cut(s) 95, 1041
FaeI CATG 6 cut(s) 106, 322, 374, 578, 795, 1184
FatI CATG 6 cut(s) 102, 318, 370, 574, 791, 1180
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 3 cut(s) 240, 430, 1005
FokI GGATG 4 cut(s) 182, 1002, 1377, 1433
FriOI GRGCYC 2 cut(s) 95, 1041
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 3 cut(s) 240, 430, 1005
FspBI CTAG 4 cut(s) 143, 183, 719, 837
GluI GCNGC 3 cut(s) 240, 430, 1005
GsaI CCCAGC 1 cut(s) 1217
GsuI CTGGAG 2 cut(s) 333, 1179
HaeIII GGCC 7 cut(s) 39, 54, 58, 285, 429, 602, 1376
HapII CCGG 3 cut(s) 55, 1175, 1352
Hin1II CATG 6 cut(s) 106, 322, 374, 578, 795, 1184
HpaII CCGG 3 cut(s) 55, 1175, 1352
HphI GGTGA 1 cut(s) 1144
Hpy166II GTNNAC 4 cut(s) 506, 592, 730, 960
Hpy188I TCNGA 4 cut(s) 531, 847, 853, 1081
Hpy8I GTNNAC 4 cut(s) 506, 592, 730, 960
Hpy99I CGWCG 1 cut(s) 1026
HpyAV CCTTC 4 cut(s) 526, 880, 1256, 1271
HpyCH4III ACNGT 2 cut(s) 274, 1288
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 7 cut(s) 215, 467, 725, 737, 773, 866, 1430
HpyF10VI GCNNNNNNNGC 1 cut(s) 1242
HpyF3I CTNAG 8 cut(s) 29, 94, 177, 439, 614, 857, 1040, 1347
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 6 cut(s) 106, 322, 374, 578, 795, 1184
Kpn2I TCCGGA 1 cut(s) 1174
KroI GCCGGC 1 cut(s) 54
KroNI GCCGGC 1 cut(s) 56
Kzo9I GATC 3 cut(s) 224, 759, 1240
LguI GCTCTTC 1 cut(s) 1179
Lsp1109I GCAGC 2 cut(s) 226, 991
LweI GCATC 4 cut(s) 160, 1355, 1417, 1439
MaeI CTAG 4 cut(s) 143, 183, 719, 837
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 4 cut(s) 415, 901, 1132, 1354
MalI GATC 3 cut(s) 226, 761, 1242
MboI GATC 3 cut(s) 224, 759, 1240
MboII GAAGA 8 cut(s) 160, 440, 489, 507, 655, 671, 822, 1196
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 3 cut(s) 95, 526, 1041
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 8 cut(s) 16, 349, 511, 629, 660, 738, 878, 1155
MluNI TGGCCA 1 cut(s) 285
MlyI GAGTC 4 cut(s) 73, 560, 935, 1129
MmeI TCCRAC 1 cut(s) 107
Mox20I TGGCCA 1 cut(s) 285
Mph1103I ATGCAT 1 cut(s) 1432
MroI TCCGGA 1 cut(s) 1174
MroNI GCCGGC 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 647
MscI TGGCCA 1 cut(s) 285
MseI TTAA 3 cut(s) 338, 1097, 1272
MslI CAYNNNNRTG 1 cut(s) 1166
Msp20I TGGCCA 1 cut(s) 285
MspI CCGG 3 cut(s) 55, 1175, 1352
MspR9I CCNGG 1 cut(s) 324
MunI CAATTG 1 cut(s) 1155
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 1242
NaeI GCCGGC 1 cut(s) 56
NdeII GATC 3 cut(s) 224, 759, 1240
NgoMIV GCCGGC 1 cut(s) 54
NlaIII CATG 6 cut(s) 106, 322, 374, 578, 795, 1184
NlaIV GGNNCC 5 cut(s) 7, 378, 490, 523, 580
NmuCI GTSAC 3 cut(s) 901, 1132, 1354
NsiI ATGCAT 1 cut(s) 1432
NspI RCATGY 1 cut(s) 322
PaeI GCATGC 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 1400
PciSI GCTCTTC 1 cut(s) 1179
PdiI GCCGGC 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 6 cut(s) 12, 676, 848, 943, 1151, 1171
PkrI GCNGC 3 cut(s) 241, 431, 1006
PleI GAGTC 4 cut(s) 73, 559, 934, 1129
PpsI GAGTC 4 cut(s) 73, 559, 934, 1129
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 2 cut(s) 299, 443
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 2 cut(s) 299, 443
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 1213
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 5 cut(s) 7, 378, 490, 523, 580
PspPPI RGGWCCY 2 cut(s) 299, 443
PspXI VCTCGAGB 1 cut(s) 1400
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 224
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 2 cut(s) 573, 1268
RsaNI GTAC 2 cut(s) 572, 1267
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 1179
SaqAI TTAA 3 cut(s) 338, 1097, 1272
SatI GCNGC 3 cut(s) 240, 430, 1005
Sau3AI GATC 3 cut(s) 224, 759, 1240
SchI GAGTC 4 cut(s) 73, 560, 935, 1129
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 3 cut(s) 95, 526, 1041
SfaNI GCATC 4 cut(s) 160, 1355, 1417, 1439
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 1400
SlaI CTCGAG 1 cut(s) 1400
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 2 cut(s) 923, 1400
SmoI CTYRAG 2 cut(s) 923, 1400
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 322
Sse9I AATT 8 cut(s) 16, 349, 511, 629, 660, 738, 878, 1155
SsiI CCGC 2 cut(s) 430, 1252
SspMI CTAG 4 cut(s) 143, 183, 719, 837
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 322
TaaI ACNGT 2 cut(s) 274, 1288
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 985, 1046, 1401
TasI AATT 8 cut(s) 16, 349, 511, 629, 660, 738, 878, 1155
TatI WGTACW 1 cut(s) 571
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 6 cut(s) 12, 676, 848, 943, 1151, 1171
Tru1I TTAA 3 cut(s) 338, 1097, 1272
Tru9I TTAA 3 cut(s) 338, 1097, 1272
TscAI CASTG 2 cut(s) 366, 1376
TseFI GTSAC 3 cut(s) 901, 1132, 1354
TseI GCWGC 2 cut(s) 239, 1004
Tsp45I GTSAC 3 cut(s) 901, 1132, 1354
TspDTI ATGAA 3 cut(s) 581, 688, 1197
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 2 cut(s) 366, 1376
XagI CCTNNNNNAGG 2 cut(s) 468, 937
XapI RAATTY 3 cut(s) 349, 511, 629
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 1400
XmiI GTMKAC 1 cut(s) 959
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 4 cut(s) 143, 183, 719, 837
Zsp2I ATGCAT 1 cut(s) 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.