Rh2BG485900

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001653
Physical Location & Seq
Forward (+)
68604967 .. 68610043
5077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG485900.1

Sequence Viewer

Length: 1047 bp
ATGGGTTCCAGATTCCCATCTCATCAGCTCAGCAATGGTCTCTACGTGTCAGGCAGGCCTGAGCAGCCAAAAGAAAGAGCACCTACAATGGGCTCAACTGCCATGCCCTATACTGGTGGTGATATCAAGAAGTCGGGAGAGCTGGGTAAAATGTTTGATATCCCGTCAATGGATGGCTCCAAAACTAGGAAATCTGGACAATTGACTGGTGCTCCTTCAAGGACCGGATCATTTGGAGGTGCTGCTTCACATTCAGGTCCTATTCCTAATGCTGCTGCTCGTTCTATCTATACCACATCTGGTCCTGTAACTTCTGGAACTGGTTCGGCTTCAATGAAGAAGACTAATTCTGGACCACTTAATAAGCATGGAGAACCTCTGAAGAAGTCATCTGGCCCTCAATCTGGTGGAGTGACCCGCCAAAATTCTGGCCAAATACCAGTACTTCCTACAACAGGCCTCATCACATCTGGTCCGATTTCTTCTGGCCCACTAAATTCCTCTGGGGCACCTAGAAAGGTATCCGGACCTTTAGACTCTATGGGATCAATGAAATTACAAGGTTCTGCTATTGTTCATAATCAAGCTGTGACTACTCTCGGCCAAGATGATTATTCTTTCAGAAAGAACTTTCCAAAGACAATTTTATGGTCTGTGATACTGATATTTGTGATGGGGTTCATCGCTGGTGGTTTTATTCTTGGAGCAGTCCACAATCCCATACTCCTAGCTGTTGTGGCAATTCTTTTCGCTGGTGTTGCTGCTTTATTCACTTGGAATACTTGTTGGGGAAGAAAAGCTACCACAAATTTCATTGCCCGCTATCCTGATGCTGAGCTTAGAACTGCTAAAAATGGGCAATATGTGAAGGTCTCGGGGGTGGTTACCTGTGGTAATGTGCCCCTCGAATCATCCTTTCGGAGAGTTCCTAGATGCGTTTATACGTCTACAAGTTTGTATGAGTACCGAGGATGGGATTCAAAACCAGCAAATCCTACACATCGGCGATTTACATGGGGACTTAGATCATCAGAGGTGAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

36.72

Weight (kDa)

10.24

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 947
AccIII TCCGGA 1 cut(s) 524
AciI CCGC 2 cut(s) 418, 820
AclWI GGATC 2 cut(s) 235, 553
AcoI YGGCCR 2 cut(s) 430, 601
AcsI RAATTY 3 cut(s) 424, 496, 808
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 2 cut(s) 444, 965
AfiI CCNNNNNNNGG 7 cut(s) 89, 113, 169, 186, 404, 455, 973
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 3 cut(s) 219, 333, 981
AluBI AGCT 7 cut(s) 28, 142, 587, 731, 800, 838, 1044
AluI AGCT 7 cut(s) 28, 142, 587, 731, 800, 838, 1044
Alw21I GWGCWC 2 cut(s) 82, 214
Alw26I GTCTC 2 cut(s) 44, 877
AlwI GGATC 2 cut(s) 235, 553
Ama87I CYCGRG 1 cut(s) 874
Aor13HI TCCGGA 1 cut(s) 524
AoxI GGCC 6 cut(s) 56, 394, 430, 457, 487, 601
ApeKI GCWGC 5 cut(s) 64, 242, 272, 275, 761
ApoI RAATTY 3 cut(s) 424, 496, 808
Asp700I GAANNNNTTC 1 cut(s) 322
AspS9I GGNCC 8 cut(s) 222, 257, 302, 353, 395, 473, 488, 527
AsuHPI GGTGA 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 874
AvaII GGWCC 6 cut(s) 222, 257, 302, 353, 473, 527
BaeGI GKGCMC 2 cut(s) 511, 903
BalI TGGCCA 1 cut(s) 432
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 95
BarI GAAGNNNNNNTAC 2 cut(s) 784, 816
BbsI GAAGAC 1 cut(s) 347
Bbv12I GWGCWC 2 cut(s) 82, 214
BbvI GCAGC 5 cut(s) 76, 229, 259, 262, 748
BccI CCATC 4 cut(s) 25, 167, 667, 966
BciVI GTATCC 1 cut(s) 532
BcoDI GTCTC 2 cut(s) 44, 877
BfaI CTAG 4 cut(s) 186, 513, 728, 930
BfuI GTATCC 1 cut(s) 532
BisI GCNGC 5 cut(s) 65, 243, 273, 276, 762
BlpI GCTNAGC 2 cut(s) 29, 834
BlsI GCNGC 5 cut(s) 66, 244, 274, 277, 763
BmcAI AGTACT 1 cut(s) 444
Bme18I GGWCC 6 cut(s) 222, 257, 302, 353, 473, 527
BmeT110I CYCGRG 1 cut(s) 874
BmgT120I GGNCC 8 cut(s) 222, 257, 302, 353, 395, 473, 488, 527
BmiI GGNNCC 3 cut(s) 7, 178, 510
BmsI GCATC 2 cut(s) 820, 923
BpiI GAAGAC 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 2 cut(s) 29, 834
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 16
BsaI GGTCTC 2 cut(s) 44, 877
BsaJI CCNNGG 1 cut(s) 967
BsaWI WCCGGW 2 cut(s) 224, 524
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 7 cut(s) 89, 113, 169, 186, 404, 455, 973
Bse1I ACTGG 4 cut(s) 118, 211, 325, 440
Bse3DI GCAATG 2 cut(s) 40, 813
Bse8I GATNNNNATC 1 cut(s) 16
BseAI TCCGGA 1 cut(s) 524
BseDI CCNNGG 1 cut(s) 967
BseGI GGATG 3 cut(s) 178, 911, 977
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 7 cut(s) 89, 113, 169, 186, 404, 455, 973
BseMI GCAATG 2 cut(s) 40, 813
BseMII CTCAG 3 cut(s) 43, 51, 825
BseNI ACTGG 4 cut(s) 118, 211, 325, 440
BseSI GKGCMC 2 cut(s) 511, 903
BseXI GCAGC 5 cut(s) 76, 229, 259, 262, 748
BseYI CCCAGC 1 cut(s) 142
BshFI GGCC 6 cut(s) 58, 396, 432, 459, 489, 603
BshNI GGYRCC 1 cut(s) 508
BsiHKAI GWGCWC 2 cut(s) 82, 214
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 2 cut(s) 225, 525
BslFI GGGAC 1 cut(s) 1032
BslI CCNNNNNNNGG 7 cut(s) 89, 113, 169, 186, 404, 455, 973
BsmAI GTCTC 2 cut(s) 44, 877
BsmFI GGGAC 1 cut(s) 1032
BsnI GGCC 6 cut(s) 58, 396, 432, 459, 489, 603
Bso31I GGTCTC 2 cut(s) 44, 877
BsoBI CYCGRG 1 cut(s) 874
Bsp1286I GDGCHC 5 cut(s) 82, 95, 214, 511, 903
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 3 cut(s) 227, 545, 1025
Bsp1720I GCTNAGC 2 cut(s) 29, 834
BspACI CCGC 2 cut(s) 418, 820
BspANI GGCC 6 cut(s) 58, 396, 432, 459, 489, 603
BspCNI CTCAG 3 cut(s) 42, 52, 826
BspEI TCCGGA 1 cut(s) 524
BspLI GGNNCC 3 cut(s) 7, 178, 510
BspPI GGATC 2 cut(s) 235, 553
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 2 cut(s) 44, 877
BsrDI GCAATG 2 cut(s) 40, 813
BsrI ACTGG 4 cut(s) 118, 211, 325, 440
BssECI CCNNGG 1 cut(s) 967
BssMI GATC 3 cut(s) 227, 545, 1025
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 56, 820
BstDEI CTNAG 5 cut(s) 29, 60, 834, 839, 1022
BstEII GGTNACC 1 cut(s) 883
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 3 cut(s) 178, 911, 977
BstKTI GATC 3 cut(s) 230, 548, 1028
BstMAI GTCTC 2 cut(s) 44, 877
BstMBI GATC 3 cut(s) 227, 545, 1025
BstMWI GCNNNNNNNGC 3 cut(s) 64, 737, 758
BstPI GGTNACC 1 cut(s) 883
BstSLI GKGCMC 2 cut(s) 511, 903
BstV1I GCAGC 5 cut(s) 76, 229, 259, 262, 748
BstV2I GAAGAC 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 428
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 6 cut(s) 58, 396, 432, 459, 489, 603
BtgZI GCGATG 1 cut(s) 667
BtsCI GGATG 3 cut(s) 178, 911, 977
Cac8I GCNNGC 2 cut(s) 56, 820
Cfr13I GGNCC 8 cut(s) 222, 257, 302, 353, 395, 473, 488, 527
Csp6I GTAC 2 cut(s) 443, 964
CviAII CATG 3 cut(s) 103, 368, 1014
CviQI GTAC 2 cut(s) 443, 964
DdeI CTNAG 5 cut(s) 29, 60, 834, 839, 1022
DpnI GATC 3 cut(s) 229, 547, 1027
DpnII GATC 3 cut(s) 227, 545, 1025
EaeI YGGCCR 2 cut(s) 430, 601
Eco147I AGGCCT 2 cut(s) 58, 459
Eco24I GRGCYC 1 cut(s) 95
Eco31I GGTCTC 2 cut(s) 44, 877
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 6 cut(s) 222, 257, 302, 353, 473, 527
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 874
Eco91I GGTNACC 1 cut(s) 883
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 883
EcoRV GATATC 2 cut(s) 124, 160
EcoT38I GRGCYC 1 cut(s) 95
FaeI CATG 3 cut(s) 106, 371, 1017
FaqI GGGAC 1 cut(s) 1032
FatI CATG 3 cut(s) 102, 367, 1013
FauI CCCGC 2 cut(s) 425, 827
FblI GTMKAC 1 cut(s) 947
Fnu4HI GCNGC 5 cut(s) 65, 243, 273, 276, 762
FokI GGATG 3 cut(s) 185, 898, 984
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 5 cut(s) 65, 243, 273, 276, 762
FspBI CTAG 4 cut(s) 186, 513, 728, 930
GluI GCNGC 5 cut(s) 65, 243, 273, 276, 762
GsaI CCCAGC 1 cut(s) 146
HaeIII GGCC 6 cut(s) 58, 396, 432, 459, 489, 603
HapII CCGG 2 cut(s) 225, 525
Hin1II CATG 3 cut(s) 106, 371, 1017
HinfI GANTC 4 cut(s) 12, 536, 908, 977
HpaII CCGG 2 cut(s) 225, 525
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 2 cut(s) 712, 948
Hpy188I TCNGA 5 cut(s) 381, 477, 623, 921, 1033
Hpy188III TCNNGA 8 cut(s) 9, 127, 135, 195, 315, 351, 525, 827
Hpy8I GTNNAC 2 cut(s) 712, 948
HpyAV CCTTC 2 cut(s) 225, 862
HpyCH4IV ACGT 2 cut(s) 45, 944
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 737, 758
HpyF3I CTNAG 5 cut(s) 29, 60, 834, 839, 1022
HpySE526I ACGT 2 cut(s) 45, 944
Hsp92II CATG 3 cut(s) 106, 371, 1017
Kpn2I TCCGGA 1 cut(s) 524
Kzo9I GATC 3 cut(s) 227, 545, 1025
LmnI GCTCC 3 cut(s) 182, 217, 704
Lsp1109I GCAGC 5 cut(s) 76, 229, 259, 262, 748
LweI GCATC 2 cut(s) 820, 923
MaeI CTAG 4 cut(s) 186, 513, 728, 930
MaeII ACGT 2 cut(s) 45, 944
MaeIII GTNAC 4 cut(s) 307, 412, 589, 883
MalI GATC 3 cut(s) 229, 547, 1027
MboI GATC 3 cut(s) 227, 545, 1025
MboII GAAGA 5 cut(s) 349, 352, 394, 474, 804
MfeI CAATTG 1 cut(s) 200
MhlI GDGCHC 5 cut(s) 82, 95, 214, 511, 903
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 8 cut(s) 200, 346, 424, 496, 554, 642, 741, 808
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 1 cut(s) 530
MnlI CCTC 8 cut(s) 230, 387, 408, 470, 511, 914, 962, 1027
Mox20I TGGCCA 1 cut(s) 432
MroI TCCGGA 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 322
MscI TGGCCA 1 cut(s) 432
MseI TTAA 1 cut(s) 360
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 2 cut(s) 225, 525
MunI CAATTG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 64, 737, 758
NdeII GATC 3 cut(s) 227, 545, 1025
NlaIII CATG 3 cut(s) 106, 371, 1017
NlaIV GGNNCC 3 cut(s) 7, 178, 510
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 2 cut(s) 412, 589
PceI AGGCCT 2 cut(s) 58, 459
PdmI GAANNNNTTC 1 cut(s) 322
PfeI GAWTC 3 cut(s) 12, 908, 977
PkrI GCNGC 5 cut(s) 66, 244, 274, 277, 763
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 257
PspEI GGTNACC 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 142
PspN4I GGNNCC 3 cut(s) 7, 178, 510
PspPI GGNCC 8 cut(s) 222, 257, 302, 353, 395, 473, 488, 527
PspPPI RGGWCCY 1 cut(s) 257
RsaI GTAC 2 cut(s) 444, 965
RsaNI GTAC 2 cut(s) 443, 964
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 5 cut(s) 65, 243, 273, 276, 762
Sau3AI GATC 3 cut(s) 227, 545, 1025
Sau96I GGNCC 8 cut(s) 222, 257, 302, 353, 395, 473, 488, 527
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 1 cut(s) 530
SduI GDGCHC 5 cut(s) 82, 95, 214, 511, 903
SfaNI GCATC 2 cut(s) 820, 923
SinI GGWCC 6 cut(s) 222, 257, 302, 353, 473, 527
Sse9I AATT 8 cut(s) 200, 346, 424, 496, 554, 642, 741, 808
SseBI AGGCCT 2 cut(s) 58, 459
SsiI CCGC 2 cut(s) 418, 820
SspMI CTAG 4 cut(s) 186, 513, 728, 930
StuI AGGCCT 2 cut(s) 58, 459
TaiI ACGT 2 cut(s) 48, 947
TaqI TCGA 1 cut(s) 906
TasI AATT 8 cut(s) 200, 346, 424, 496, 554, 642, 741, 808
TatI WGTACW 1 cut(s) 442
TfiI GAWTC 3 cut(s) 12, 908, 977
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TseFI GTSAC 2 cut(s) 412, 589
TseI GCWGC 5 cut(s) 64, 242, 272, 275, 761
Tsp45I GTSAC 2 cut(s) 412, 589
TspDTI ATGAA 5 cut(s) 350, 566, 566, 670, 802
VpaK11BI GGWCC 6 cut(s) 222, 257, 302, 353, 473, 527
XagI CCTNNNNNAGG 1 cut(s) 453
XapI RAATTY 3 cut(s) 424, 496, 808
XmiI GTMKAC 1 cut(s) 947
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 4 cut(s) 186, 513, 728, 930
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.