Rorug02G0413700

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001653
Physical Location & Seq
Forward (+)
52986192 .. 52989337
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0413700.1

Sequence Viewer

Length: 576 bp
ATGAGTCATTATAACTTGCAACTCTTAGGCAACCTATCTGAAGTCAATGATAGCCAAGTTGTTAGCCAACACACACAAAATGACGATGAAGGTGAAGAAATTCTTATGAGGAAGATTAGGGGAGATCATAACATGACACCAAACCATGCCGATCGCGTGGTGAATGAGATGAATGCATCTAGAGGATCTGTTAATAGAAATATCAGAGATATGAGATCAAAAACTGGACATGGGACCAGGAAGAGGGCTGGAAAAATCAAGAAACACAGGGGTAAAAATAAGGTTAACTTTGGTCCTTATGGCCCTGAGAAGATGCTGTTTAACAAGTTTGGGAATTTTAAAGGGACCGGAAGGGTTGAAAGTGCATGGGCAGAAATAAAGGCAAAAATAGATTGGTCTGAGCCACAAACCTTGGCAATTATGAAAAGAATTCATAAGAAAAAATCAGTGACTACCAAGAAGAATAGAAGTCAGTTGAAGGTGGCTGCAAACACCGGAACAAAGACATTTGCCCAAATACGATATGAATGGGAACTTGCAAACGAAGGGAAAGAAATAGATCGCTGCACATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.87

Weight (kDa)

10.1

Isoelectric Point (pI)

33.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
AccII CGCG 1 cut(s) 156
AclWI GGATC 1 cut(s) 193
AcsI RAATTY 3 cut(s) 99, 334, 429
AcuI CTGAAG 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 243
AgsI TTSAA 2 cut(s) 359, 478
AjnI CCWGG 1 cut(s) 236
AlwI GGATC 1 cut(s) 193
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 2 cut(s) 485, 564
ApoI RAATTY 3 cut(s) 99, 334, 429
Asp700I GAANNNNTTC 1 cut(s) 99
AspS9I GGNCC 4 cut(s) 234, 293, 302, 345
AsuHPI GGTGA 2 cut(s) 104, 172
AvaII GGWCC 3 cut(s) 234, 293, 345
BbvI GCAGC 2 cut(s) 472, 551
BciT130I CCWGG 1 cut(s) 238
BfaI CTAG 1 cut(s) 180
BisI GCNGC 2 cut(s) 486, 565
BlsI GCNGC 2 cut(s) 487, 566
Bme1390I CCNGG 1 cut(s) 238
Bme18I GGWCC 3 cut(s) 234, 293, 345
BmgT120I GGNCC 4 cut(s) 234, 293, 302, 345
BmiI GGNNCC 2 cut(s) 235, 346
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 185, 303
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 2 cut(s) 347, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 229
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 2 cut(s) 297, 390
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 472, 551
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 348, 495
BslFI GGGAC 2 cut(s) 247, 358
BslI CCNNNNNNNGG 1 cut(s) 243
BsmFI GGGAC 2 cut(s) 247, 358
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 303
Bsp143I GATC 5 cut(s) 124, 151, 185, 215, 559
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 2 cut(s) 298, 391
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 235, 346
BspPI GGATC 1 cut(s) 193
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 5 cut(s) 124, 151, 185, 215, 559
BssT1I CCWWGG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 236
BstDEI CTNAG 3 cut(s) 25, 306, 399
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 5 cut(s) 127, 154, 188, 218, 562
BstMBI GATC 5 cut(s) 124, 151, 185, 215, 559
BstMCI CGRYCG 1 cut(s) 154
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 472, 551
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 1 cut(s) 303
BtsIMutI CAGTG 1 cut(s) 453
Cfr13I GGNCC 4 cut(s) 234, 293, 302, 345
CspCI CAANNNNNGTGG 2 cut(s) 393, 428
CviAII CATG 4 cut(s) 133, 146, 230, 366
CviJI RGCY 6 cut(s) 54, 66, 248, 303, 403, 485
CviKI_1 RGCY 6 cut(s) 54, 66, 248, 303, 403, 485
DdeI CTNAG 3 cut(s) 25, 306, 399
DpnI GATC 5 cut(s) 126, 153, 187, 217, 561
DpnII GATC 5 cut(s) 124, 151, 185, 215, 559
DraI TTTAAA 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 411
Eco47I GGWCC 3 cut(s) 234, 293, 345
Eco57I CTGAAG 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 411
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 4 cut(s) 136, 149, 233, 369
FalI AAGNNNNNCTT 4 cut(s) 87, 119, 272, 304
FaqI GGGAC 2 cut(s) 247, 358
FatI CATG 4 cut(s) 132, 145, 229, 365
Fnu4HI GCNGC 2 cut(s) 486, 565
Fsp4HI GCNGC 2 cut(s) 486, 565
FspBI CTAG 1 cut(s) 180
GluI GCNGC 2 cut(s) 486, 565
HaeIII GGCC 1 cut(s) 303
HapII CCGG 2 cut(s) 348, 495
Hin1II CATG 4 cut(s) 136, 149, 233, 369
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 4
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 2 cut(s) 348, 495
HphI GGTGA 2 cut(s) 104, 172
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 4 cut(s) 40, 206, 400, 575
Hpy188III TCNNGA 2 cut(s) 180, 259
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 4 cut(s) 83, 345, 472, 539
HpyCH4V TGCA 6 cut(s) 19, 176, 365, 488, 539, 567
HpyF3I CTNAG 3 cut(s) 25, 306, 399
Hsp92II CATG 4 cut(s) 136, 149, 233, 369
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 5 cut(s) 124, 151, 185, 215, 559
LpnPI CCDG 8 cut(s) 210, 223, 234, 250, 253, 318, 361, 508
Lsp1109I GCAGC 2 cut(s) 472, 551
LweI GCATC 2 cut(s) 185, 303
MaeI CTAG 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 448
MalI GATC 5 cut(s) 126, 153, 187, 217, 561
MboI GATC 5 cut(s) 124, 151, 185, 215, 559
MboII GAAGA 5 cut(s) 107, 124, 253, 322, 472
MflI RGATCY 1 cut(s) 185
MluCI AATT 4 cut(s) 99, 334, 417, 429
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 3 cut(s) 102, 176, 237
Mph1103I ATGCAT 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 4 cut(s) 192, 285, 321, 339
MspI CCGG 2 cut(s) 348, 495
MspR9I CCNGG 1 cut(s) 238
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 1 cut(s) 156
NdeII GATC 5 cut(s) 124, 151, 185, 215, 559
NlaIII CATG 4 cut(s) 136, 149, 233, 369
NlaIV GGNNCC 2 cut(s) 235, 346
NmuCI GTSAC 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 178
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 99
PkrI GCNGC 2 cut(s) 487, 566
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PsiI TTATAA 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 235, 346
PspPI GGNCC 4 cut(s) 234, 293, 302, 345
PsuI RGATCY 1 cut(s) 185
PvuI CGATCG 1 cut(s) 154
SaqAI TTAA 4 cut(s) 192, 285, 321, 339
SatI GCNGC 2 cut(s) 486, 565
Sau3AI GATC 5 cut(s) 124, 151, 185, 215, 559
Sau96I GGNCC 4 cut(s) 234, 293, 302, 345
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 238
SetI ASST 5 cut(s) 36, 94, 285, 413, 483
SfaNI GCATC 2 cut(s) 185, 303
SinI GGWCC 3 cut(s) 234, 293, 345
Sse9I AATT 4 cut(s) 99, 334, 417, 429
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 411
TasI AATT 4 cut(s) 99, 334, 417, 429
Tru1I TTAA 4 cut(s) 192, 285, 321, 339
Tru9I TTAA 4 cut(s) 192, 285, 321, 339
TscAI CASTG 1 cut(s) 453
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 2 cut(s) 485, 564
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 5 cut(s) 102, 185, 422, 437, 540
TspRI CASTG 1 cut(s) 453
VpaK11BI GGWCC 3 cut(s) 234, 293, 345
XapI RAATTY 3 cut(s) 99, 334, 429
XbaI TCTAGA 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.