pycom09g08080

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001653
Physical Location & Seq
Reverse (-)
6199501 .. 6202588
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g08080.4

Sequence Viewer

Length: 1458 bp
ATGGGTTCCAGATTCAATTCTCATCAACTCAGCAATGGCCTTTACGTTTCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCTGTGCCCTATACTGGTGGAGATATCAAGAAGTCGGGAGAACTAGGGAAGATGTTTGATATCCCTACGGATGGCTCTAAGTCTAGAAAATCTGGACCTATAACTGGTGCTCCTTCAAGGACTGGATCTTTTGGAGGTGCTGCTTCACATTCAGGACCAATCCCTAATTCTGCAGCTCGTGCAGGCTATACCACATCAGGTCCCGTATCATCTGGAGGCATGCCCGGTTCTGCTTCCTTAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCTCTAAAGAAGTCGTCTGGTCCACAATCTGGTGGGGTAACACCAACTGGCCGGCCAAACTCTGGACCTCTTCCTCCTGTTCTCCCTGCAACAGGTCTCATTACATCTGGGCCCATTTCTTCTGGCCCACTAAATTCTTCTGGTGCTCCTCGTAAGGTATCTGGTCCTTTGGAGTCAATGGGTTCAATGAAAGTACACGGTTCCTCCATTGGTCACAATCAGGCCATTACTACTCTCAGCCAAGATGACGAGTTTTCGTTTAGGAAGAACTTCCCAAAGTTAATTATGTGGTCTTTGATTCTTCTTTTTGTGATGGGGTTCATAGCTGGTGGTTTTATTCTAGGCGCAGTCCACAATGCAATTCTCCTCATCGTGGTTGCGATCCTTTTCGGTGCAGTTGCTACACTATTCACATGGAATACTTATTGGGGAAGACGAGCTATTATAGGTTATATTGCTAGTTATTCAGATTCTGAGCTGAGGACTGCAAAGAATGGGCAATTTGTGAAGGTCTCAGGGGTGGTCACTTGTGGCAATCTGCCTCTTGAGTCGTCCTTTGAAAAAATTCCTAGATGTGTGTATACATCTACCAGTTTATATGAGTATCGAGGATGGGATTCAAAAGCTGCCAACCCTACACATCGTCGATTTACTTGGGGGCTCAGGTCCATAGAAAGGCGTGTGGTAGATTTCTACATCTCTGATTTCCAATCTGGGTTAAGAGCATTGGTTAAGACTGGTTCTGGGGCAAGGGTGACTCCTTATGTTGAAGATTCGATTGTCATTGATGTGAATCCTGAAAATGAAGAGTTGTCCCCAGAGTTTATTAGGTGGTTGGGAGAGAGGAATCTTTCAAGTGATGACCGCATGATGCGGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTTAGTGTAATGGGAGTCGTCCAAAGAAATGAAAATGTGCTGATGATTGTTCCTCCACCTGAGCCGATCACGACAGGATGCCAGTGGGCCAAATGTATCTTTCCAGCCAGCCTCGAGGGTATTGTGTTGAGATGTGATGATGCATCAAAGAATGATGTCATCCCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

486

Amino Acids

51.82

Weight (kDa)

9.47

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1400
AccB7I CCANNNNNTGG 2 cut(s) 407, 440
AccI GTMKAC 1 cut(s) 959
AciI CCGC 2 cut(s) 1243, 1252
AclWI GGATC 2 cut(s) 232, 754
AcoI YGGCCR 3 cut(s) 56, 427, 431
AcsI RAATTY 3 cut(s) 349, 511, 942
AfaI GTAC 2 cut(s) 573, 1268
AfiI CCNNNNNNNGG 8 cut(s) 113, 170, 203, 407, 440, 470, 751, 1053
AgsI TTSAA 8 cut(s) 16, 216, 564, 938, 999, 1148, 1233, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 250, 282
AluBI AGCT 6 cut(s) 93, 275, 704, 818, 856, 1004
AluI AGCT 6 cut(s) 93, 275, 704, 818, 856, 1004
Alw21I GWGCWC 3 cut(s) 95, 211, 526
Alw26I GTCTC 2 cut(s) 479, 895
AlwI GGATC 2 cut(s) 232, 754
AlwNI CAGNNNCTG 2 cut(s) 101, 851
Ama87I CYCGRG 1 cut(s) 1400
AoxI GGCC 9 cut(s) 37, 52, 56, 427, 431, 488, 502, 600, 1374
ApaI GGGCCC 1 cut(s) 492
ApeKI GCWGC 3 cut(s) 239, 272, 1004
ApoI RAATTY 3 cut(s) 349, 511, 942
Asp700I GAANNNNTTC 2 cut(s) 647, 942
AspLEI GCGC 1 cut(s) 725
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 1 cut(s) 1144
AvaI CYCGRG 1 cut(s) 1400
AvaII GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
BaeGI GKGCMC 2 cut(s) 108, 492
BanII GRGCYC 3 cut(s) 95, 492, 1041
BauI CACGAG 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 817
Bbv12I GWGCWC 3 cut(s) 95, 211, 526
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 3 cut(s) 226, 284, 991
BccI CCATC 3 cut(s) 164, 685, 984
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 479, 895
BfaI CTAG 5 cut(s) 143, 183, 719, 837, 948
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 3 cut(s) 240, 273, 1005
BlsI GCNGC 3 cut(s) 241, 274, 1006
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
BmeT110I CYCGRG 1 cut(s) 1400
BmiI GGNNCC 5 cut(s) 7, 301, 378, 490, 580
BmrFI CCNGG 1 cut(s) 324
BmrI ACTGGG 1 cut(s) 1445
BmsI GCATC 4 cut(s) 1239, 1355, 1417, 1439
BmuI ACTGGG 1 cut(s) 1445
BpiI GAAGAC 1 cut(s) 817
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 3 cut(s) 857, 1040, 1347
BpuEI CTTGAG 1 cut(s) 944
BpuMI CCSGG 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 1170
BsaI GGTCTC 2 cut(s) 479, 895
BsaXI ACNNNNNCTCC 4 cut(s) 193, 223, 566, 596
Bsc4I CCNNNNNNNGG 8 cut(s) 113, 170, 203, 407, 440, 470, 751, 1053
Bse118I RCCGGY 2 cut(s) 54, 429
Bse1I ACTGG 8 cut(s) 118, 208, 226, 430, 969, 1120, 1369, 1451
Bse3DI GCAATG 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 1170
BseGI GGATG 4 cut(s) 175, 995, 1370, 1446
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 8 cut(s) 113, 170, 203, 407, 440, 470, 751, 1053
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 8 cut(s) 43, 108, 628, 843, 848, 906, 1054, 1338
BseNI ACTGG 8 cut(s) 118, 208, 226, 430, 969, 1120, 1369, 1451
BseRI GAGGAG 2 cut(s) 516, 734
BseSI GKGCMC 2 cut(s) 108, 492
BseXI GCAGC 3 cut(s) 226, 284, 991
BsgI GTGCAG 2 cut(s) 300, 792
BshFI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
BsiHKAI GWGCWC 3 cut(s) 95, 211, 526
BsiHKCI CYCGRG 1 cut(s) 1400
BsiSI CCGG 3 cut(s) 55, 324, 430
BslFI GGGAC 2 cut(s) 285, 1177
BslI CCNNNNNNNGG 8 cut(s) 113, 170, 203, 407, 440, 470, 751, 1053
BsmAI GTCTC 2 cut(s) 479, 895
BsmFI GGGAC 2 cut(s) 285, 1177
BsnI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
Bso31I GGTCTC 2 cut(s) 479, 895
BsoBI CYCGRG 1 cut(s) 1400
Bsp120I GGGCCC 1 cut(s) 488
Bsp1286I GDGCHC 6 cut(s) 95, 108, 211, 492, 526, 1041
Bsp143I GATC 3 cut(s) 224, 759, 1353
BspACI CCGC 2 cut(s) 1243, 1252
BspANI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
BspCNI CTCAG 8 cut(s) 42, 107, 627, 844, 849, 905, 1053, 1339
BspLI GGNNCC 5 cut(s) 7, 301, 378, 490, 580
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 2 cut(s) 232, 754
BspTNI GGTCTC 2 cut(s) 479, 895
BsrDI GCAATG 1 cut(s) 40
BsrFI RCCGGY 2 cut(s) 54, 429
BsrI ACTGG 8 cut(s) 118, 208, 226, 430, 969, 1120, 1369, 1451
BssAI RCCGGY 2 cut(s) 54, 429
BssMI GATC 3 cut(s) 224, 759, 1353
BssNAI GTATAC 1 cut(s) 960
BssSI CACGAG 1 cut(s) 276
Bst1107I GTATAC 1 cut(s) 960
Bst2BI CACGAG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 578, 1288
Bst6I CTCTTC 2 cut(s) 453, 1179
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 5 cut(s) 56, 283, 320, 431, 1396
BstDEI CTNAG 9 cut(s) 29, 94, 177, 614, 852, 857, 892, 1040, 1347
BstENI CCTNNNNNAGG 1 cut(s) 468
BstF5I GGATG 4 cut(s) 175, 995, 1370, 1446
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 3 cut(s) 227, 762, 1356
BstMAI GTCTC 2 cut(s) 479, 895
BstMBI GATC 3 cut(s) 224, 759, 1353
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 2 cut(s) 108, 492
BstV1I GCAGC 3 cut(s) 226, 284, 991
BstV2I GAAGAC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 960
BsuRI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
BtsCI GGATG 4 cut(s) 175, 995, 1370, 1446
BtsIMutI CAGTG 2 cut(s) 359, 1376
Cac8I GCNNGC 5 cut(s) 56, 283, 320, 431, 1396
CaiI CAGNNNCTG 2 cut(s) 101, 851
CfoI GCGC 1 cut(s) 725
Cfr10I RCCGGY 2 cut(s) 54, 429
Csp6I GTAC 2 cut(s) 572, 1267
CviAII CATG 4 cut(s) 319, 371, 792, 1246
CviQI GTAC 2 cut(s) 572, 1267
DdeI CTNAG 9 cut(s) 29, 94, 177, 614, 852, 857, 892, 1040, 1347
DpnI GATC 3 cut(s) 226, 761, 1355
DpnII GATC 3 cut(s) 224, 759, 1353
EaeI YGGCCR 3 cut(s) 56, 427, 431
Eam1104I CTCTTC 2 cut(s) 453, 1179
EarI CTCTTC 2 cut(s) 453, 1179
Ecl136II GAGCTC 1 cut(s) 93
Eco24I GRGCYC 3 cut(s) 95, 492, 1041
Eco31I GGTCTC 2 cut(s) 479, 895
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
Eco53kI GAGCTC 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 1400
EcoICRI GAGCTC 1 cut(s) 93
EcoNI CCTNNNNNAGG 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 299
EcoRV GATATC 2 cut(s) 124, 160
EcoT22I ATGCAT 1 cut(s) 1432
EcoT38I GRGCYC 3 cut(s) 95, 492, 1041
FaeI CATG 4 cut(s) 322, 374, 795, 1249
FaqI GGGAC 2 cut(s) 285, 1177
FatI CATG 4 cut(s) 318, 370, 791, 1245
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 3 cut(s) 240, 273, 1005
FokI GGATG 4 cut(s) 182, 1002, 1377, 1433
FriOI GRGCYC 3 cut(s) 95, 492, 1041
FseI GGCCGGCC 2 cut(s) 58, 433
Fsp4HI GCNGC 3 cut(s) 240, 273, 1005
FspBI CTAG 5 cut(s) 143, 183, 719, 837, 948
GlaI GCGC 1 cut(s) 724
GluI GCNGC 3 cut(s) 240, 273, 1005
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
HapII CCGG 3 cut(s) 55, 324, 430
HhaI GCGC 1 cut(s) 725
Hin1II CATG 4 cut(s) 322, 374, 795, 1249
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HpaII CCGG 3 cut(s) 55, 324, 430
HphI GGTGA 1 cut(s) 1144
Hpy166II GTNNAC 4 cut(s) 401, 574, 730, 960
Hpy188I TCNGA 3 cut(s) 847, 853, 1081
Hpy8I GTNNAC 4 cut(s) 401, 574, 730, 960
Hpy99I CGWCG 1 cut(s) 1026
HpyAV CCTTC 4 cut(s) 222, 880, 1256, 1271
HpyCH4III ACNGT 2 cut(s) 578, 1288
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 7 cut(s) 272, 281, 467, 737, 773, 866, 1430
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 9 cut(s) 29, 94, 177, 614, 852, 857, 892, 1040, 1347
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 4 cut(s) 322, 374, 795, 1249
HspAI GCGC 1 cut(s) 723
KroI GCCGGC 2 cut(s) 54, 429
KroNI GCCGGC 2 cut(s) 56, 431
Kzo9I GATC 3 cut(s) 224, 759, 1353
LmnI GCTCC 2 cut(s) 214, 529
Lsp1109I GCAGC 3 cut(s) 226, 284, 991
LweI GCATC 4 cut(s) 1239, 1355, 1417, 1439
MaeI CTAG 5 cut(s) 143, 183, 719, 837, 948
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 4 cut(s) 415, 590, 901, 1132
MalI GATC 3 cut(s) 226, 761, 1355
MboI GATC 3 cut(s) 224, 759, 1353
MboII GAAGA 9 cut(s) 160, 440, 489, 507, 655, 671, 822, 1160, 1196
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 6 cut(s) 95, 108, 211, 492, 526, 1041
MluCI AATT 8 cut(s) 16, 265, 349, 511, 660, 738, 878, 942
MlyI GAGTC 5 cut(s) 73, 560, 935, 1129, 1311
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 1432
MroNI GCCGGC 2 cut(s) 54, 429
MroXI GAANNNNTTC 2 cut(s) 647, 942
MseI TTAA 5 cut(s) 338, 659, 1097, 1110, 1272
MslI CAYNNNNRTG 1 cut(s) 1166
MspI CCGG 3 cut(s) 55, 324, 430
MspR9I CCNGG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 278
NaeI GCCGGC 2 cut(s) 56, 431
NciI CCSGG 1 cut(s) 324
NdeII GATC 3 cut(s) 224, 759, 1353
NgoMIV GCCGGC 2 cut(s) 54, 429
NlaIII CATG 4 cut(s) 322, 374, 795, 1249
NlaIV GGNNCC 5 cut(s) 7, 301, 378, 490, 580
NmuCI GTSAC 3 cut(s) 590, 901, 1132
NsiI ATGCAT 1 cut(s) 1432
NspI RCATGY 1 cut(s) 322
PaeI GCATGC 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 1400
PdiI GCCGGC 2 cut(s) 56, 431
PdmI GAANNNNTTC 2 cut(s) 647, 942
PfeI GAWTC 7 cut(s) 12, 676, 848, 995, 1151, 1171, 1225
PflMI CCANNNNNTGG 2 cut(s) 407, 440
PkrI GCNGC 3 cut(s) 241, 274, 1006
PleI GAGTC 5 cut(s) 73, 559, 934, 1129, 1310
PpsI GAGTC 5 cut(s) 73, 559, 934, 1129, 1310
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 299
PspN4I GGNNCC 5 cut(s) 7, 301, 378, 490, 580
PspOMI GGGCCC 1 cut(s) 488
PspPPI RGGWCCY 1 cut(s) 299
PspXI VCTCGAGB 1 cut(s) 1400
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 2 cut(s) 101, 851
PsuI RGATCY 1 cut(s) 224
RigI GGCCGGCC 2 cut(s) 58, 433
RsaI GTAC 2 cut(s) 573, 1268
RsaNI GTAC 2 cut(s) 572, 1267
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 1 cut(s) 95
SaqAI TTAA 5 cut(s) 338, 659, 1097, 1110, 1272
SatI GCNGC 3 cut(s) 240, 273, 1005
Sau3AI GATC 3 cut(s) 224, 759, 1353
SchI GAGTC 5 cut(s) 73, 560, 935, 1129, 1311
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 6 cut(s) 95, 108, 211, 492, 526, 1041
SfaNI GCATC 4 cut(s) 1239, 1355, 1417, 1439
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 1400
SinI GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
SlaI CTCGAG 1 cut(s) 1400
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 2 cut(s) 923, 1400
SmoI CTYRAG 2 cut(s) 923, 1400
SphI GCATGC 1 cut(s) 322
Sse9I AATT 8 cut(s) 16, 265, 349, 511, 660, 738, 878, 942
SsiI CCGC 2 cut(s) 1243, 1252
SspMI CTAG 5 cut(s) 143, 183, 719, 837, 948
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 322
TaaI ACNGT 2 cut(s) 578, 1288
TaiI ACGT 1 cut(s) 48
TaqI TCGA 4 cut(s) 985, 1024, 1154, 1401
TasI AATT 8 cut(s) 16, 265, 349, 511, 660, 738, 878, 942
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 7 cut(s) 12, 676, 848, 995, 1151, 1171, 1225
Tru1I TTAA 5 cut(s) 338, 659, 1097, 1110, 1272
Tru9I TTAA 5 cut(s) 338, 659, 1097, 1110, 1272
TscAI CASTG 2 cut(s) 366, 1376
TseFI GTSAC 3 cut(s) 590, 901, 1132
TseI GCWGC 3 cut(s) 239, 272, 1004
Tsp45I GTSAC 3 cut(s) 590, 901, 1132
TspDTI ATGAA 4 cut(s) 581, 688, 1197, 1332
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 2 cut(s) 366, 1376
Van91I CCANNNNNTGG 2 cut(s) 407, 440
VpaK11BI GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
XagI CCTNNNNNAGG 1 cut(s) 468
XapI RAATTY 3 cut(s) 349, 511, 942
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 1400
XmiI GTMKAC 1 cut(s) 959
XmnI GAANNNNTTC 2 cut(s) 647, 942
XspI CTAG 5 cut(s) 143, 183, 719, 837, 948
Zsp2I ATGCAT 1 cut(s) 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.