MD09G1163300.v1.1

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001653
Physical Location & Seq
Reverse (-)
13320131 .. 13325075
4945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1163300.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGGTTCCAGATTCAATTCTCATCAACTCAGCAATGGCCTTTACGTATCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCCGTGCCCTATACTGGTGGAGATATCAAGAAGTCGGGAGAACTAGGGAAGATGTTTGATATCCCTACGGATGGCTCTAAGTCTAGAAAATCTGGACCTATAACTGGTGCTTCTTCAAGGACTGGATCTTTTGGAGGTGCTGCTTCACATTCAGGACCAATCCCTAATTCTGCAGCTCGTGCAGGCTATACCACATCAGGTCCCGTATCATCTGGAGGCATGCCCGGTTCTGCTTCCTTAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCTTTAAAGAAGTTGTCTGGTCCACAATCTGGTGGGGTAACACCAACTGGCCGGCCAAACTCTGGACCTCTTCCTCCTGTTCTCCCTGCAACCGGTCTCATTACATCTGGGCCCATTTCTTCTGGCCCACTAAATTCTTCTGGTGCCCCTCGTAAGGTATCTGGTCCTTTAGAGTCAATGGGTTCAATGAAAGTACACGGTTCCTCCATTGTTCACAATCAGGCCATTACTACTCTCAGCCAAGATGACGAGTTTTCATTTAAGAAGAACTTCCCAAAGTTAATTATGTGGTCTTTGATTCTTCTGTTCGTGATGGGGTTCATAGCTGGTGGTTTTATTCTAGGTGCAGTCCACAATGCAATTCTCCTTATCGTGGTTGTGATCCTTTTCGGTCTAGTTGCTACACTATTCACATGGAATACTTATTGGGGAAGACGAGCTATTATAGGTTATATTGCTAGTTATTCAGATTCCGAGCTGAGGACTGCAAAGAATGGGCAATTTGTGAAGGTCTCAGGGGTGGTCACTTGTGGCAATCTACCTCTTGAGTCGTCCTTTCAAAAAATTCCTAGATGTGTGTATACATCTACCAGTTTATATGAGTATCGAGGATGGGATTCAAAAGCTGCCAACCCTACACATCGTCGATTTACTTGGGGGCTCAGGTCCATAGAAAGGCGTTTGGTAGATTTCTACATCTCTGATTTCCAATCTGGGTTAAGAGCATTGGTTAAGACTGGTTCTGGGGCAAGGGTGACTCCTTATGTTGAAGATTCGTTTGTCATTGATGTGAATCCTGAAAATGAAGAGTTGTCTCCAGAGTTTATTAGGTGGTTGGGAGAGAGGAATCTTTCAAGTGATGACCGCATGATGCGGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTTAGTGTAATGGGAGTCGTCCAAAGAAATGAAAATGTGCTGATGATTGTTCCTCCACCTGAGCCGATCACGACAGGATGCCAGTGGGCCAAATGTATCTTTCCAGCCAGCGTCGAGGGTATTGTGTTGAGATGTGATGATGCATCAAAGAATGATGTCATCCCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

486

Amino Acids

51.95

Weight (kDa)

9.51

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 521
AccB7I CCANNNNNTGG 2 cut(s) 407, 440
AccI GTMKAC 1 cut(s) 959
AciI CCGC 2 cut(s) 1243, 1252
AclWI GGATC 2 cut(s) 232, 754
AcoI YGGCCR 3 cut(s) 56, 427, 431
AcsI RAATTY 3 cut(s) 349, 511, 942
AfaI GTAC 2 cut(s) 573, 1268
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 8 cut(s) 16, 216, 564, 938, 999, 1148, 1233, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 250, 282
AluBI AGCT 6 cut(s) 93, 275, 704, 818, 856, 1004
AluI AGCT 6 cut(s) 93, 275, 704, 818, 856, 1004
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 3 cut(s) 479, 895, 1197
AlwI GGATC 2 cut(s) 232, 754
AoxI GGCC 9 cut(s) 37, 52, 56, 427, 431, 488, 502, 600, 1374
ApaI GGGCCC 1 cut(s) 492
ApeKI GCWGC 3 cut(s) 239, 272, 1004
ApoI RAATTY 3 cut(s) 349, 511, 942
AsiGI ACCGGT 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 647
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 1 cut(s) 1144
AvaII GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
BaeGI GKGCMC 3 cut(s) 108, 492, 526
BanI GGYRCC 1 cut(s) 521
BanII GRGCYC 3 cut(s) 95, 492, 1041
BauI CACGAG 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 817
Bbv12I GWGCWC 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 3 cut(s) 226, 284, 991
BccI CCATC 3 cut(s) 164, 685, 984
BceAI ACGGC 1 cut(s) 86
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 3 cut(s) 479, 895, 1197
BfaI CTAG 6 cut(s) 143, 183, 719, 773, 837, 948
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 3 cut(s) 240, 273, 1005
BlsI GCNGC 3 cut(s) 241, 274, 1006
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
BmiI GGNNCC 6 cut(s) 7, 301, 378, 490, 523, 580
BmrFI CCNGG 1 cut(s) 324
BmrI ACTGGG 1 cut(s) 1445
BmsI GCATC 4 cut(s) 1239, 1355, 1417, 1439
BmuI ACTGGG 1 cut(s) 1445
BpiI GAAGAC 1 cut(s) 817
BpmI CTGGAG 2 cut(s) 333, 1179
Bpu10I CCTNAGC 3 cut(s) 857, 1040, 1347
BpuEI CTTGAG 1 cut(s) 944
BpuMI CCSGG 1 cut(s) 324
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 1170
BsaI GGTCTC 2 cut(s) 479, 895
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bse118I RCCGGY 3 cut(s) 54, 429, 470
Bse1I ACTGG 8 cut(s) 118, 208, 226, 430, 969, 1120, 1369, 1451
Bse3DI GCAATG 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 1170
BseGI GGATG 4 cut(s) 175, 995, 1370, 1446
BseJI GATNNNNATC 1 cut(s) 1170
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 7 cut(s) 43, 108, 628, 848, 906, 1054, 1338
BseNI ACTGG 8 cut(s) 118, 208, 226, 430, 969, 1120, 1369, 1451
BseSI GKGCMC 3 cut(s) 108, 492, 526
BseXI GCAGC 3 cut(s) 226, 284, 991
BsgI GTGCAG 2 cut(s) 300, 744
BshFI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
BshNI GGYRCC 1 cut(s) 521
BshTI ACCGGT 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 4 cut(s) 55, 324, 430, 471
BslFI GGGAC 1 cut(s) 285
BsmAI GTCTC 3 cut(s) 479, 895, 1197
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
Bso31I GGTCTC 2 cut(s) 479, 895
Bsp120I GGGCCC 1 cut(s) 488
Bsp1286I GDGCHC 5 cut(s) 95, 108, 492, 526, 1041
Bsp143I GATC 3 cut(s) 224, 759, 1353
BspACI CCGC 2 cut(s) 1243, 1252
BspANI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
BspCNI CTCAG 7 cut(s) 42, 107, 627, 849, 905, 1053, 1339
BspLI GGNNCC 6 cut(s) 7, 301, 378, 490, 523, 580
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 2 cut(s) 232, 754
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 479, 895
BsrDI GCAATG 1 cut(s) 40
BsrFI RCCGGY 3 cut(s) 54, 429, 470
BsrI ACTGG 8 cut(s) 118, 208, 226, 430, 969, 1120, 1369, 1451
BssAI RCCGGY 3 cut(s) 54, 429, 470
BssMI GATC 3 cut(s) 224, 759, 1353
BssNAI GTATAC 1 cut(s) 960
BssSI CACGAG 1 cut(s) 276
Bst1107I GTATAC 1 cut(s) 960
Bst2BI CACGAG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 578, 1288
Bst6I CTCTTC 2 cut(s) 453, 1179
BstAPI GCANNNNNTGC 1 cut(s) 278
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 5 cut(s) 56, 283, 320, 431, 1396
BstDEI CTNAG 8 cut(s) 29, 94, 177, 614, 857, 892, 1040, 1347
BstF5I GGATG 4 cut(s) 175, 995, 1370, 1446
BstKTI GATC 3 cut(s) 227, 762, 1356
BstMAI GTCTC 3 cut(s) 479, 895, 1197
BstMBI GATC 3 cut(s) 224, 759, 1353
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 3 cut(s) 108, 492, 526
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 226, 284, 991
BstV2I GAAGAC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 960
BsuRI GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
BtsCI GGATG 4 cut(s) 175, 995, 1370, 1446
BtsIMutI CAGTG 2 cut(s) 359, 1376
Cac8I GCNNGC 5 cut(s) 56, 283, 320, 431, 1396
Cfr10I RCCGGY 3 cut(s) 54, 429, 470
CseI GACGC 1 cut(s) 1387
Csp6I GTAC 2 cut(s) 572, 1267
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 4 cut(s) 319, 371, 792, 1246
CviQI GTAC 2 cut(s) 572, 1267
DdeI CTNAG 8 cut(s) 29, 94, 177, 614, 857, 892, 1040, 1347
DpnI GATC 3 cut(s) 226, 761, 1355
DpnII GATC 3 cut(s) 224, 759, 1353
DraI TTTAAA 1 cut(s) 384
EaeI YGGCCR 3 cut(s) 56, 427, 431
Eam1104I CTCTTC 2 cut(s) 453, 1179
EarI CTCTTC 2 cut(s) 453, 1179
Ecl136II GAGCTC 1 cut(s) 93
Eco105I TACGTA 1 cut(s) 46
Eco24I GRGCYC 3 cut(s) 95, 492, 1041
Eco31I GGTCTC 2 cut(s) 479, 895
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
Eco53kI GAGCTC 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 93
EcoO109I RGGNCCY 1 cut(s) 299
EcoRV GATATC 2 cut(s) 124, 160
EcoT22I ATGCAT 1 cut(s) 1432
EcoT38I GRGCYC 3 cut(s) 95, 492, 1041
FaeI CATG 4 cut(s) 322, 374, 795, 1249
FalI AAGNNNNNCTT 2 cut(s) 632, 664
FaqI GGGAC 1 cut(s) 285
FatI CATG 4 cut(s) 318, 370, 791, 1245
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 3 cut(s) 240, 273, 1005
FokI GGATG 4 cut(s) 182, 1002, 1377, 1433
FriOI GRGCYC 3 cut(s) 95, 492, 1041
FseI GGCCGGCC 2 cut(s) 58, 433
Fsp4HI GCNGC 3 cut(s) 240, 273, 1005
FspBI CTAG 6 cut(s) 143, 183, 719, 773, 837, 948
GluI GCNGC 3 cut(s) 240, 273, 1005
GsuI CTGGAG 2 cut(s) 333, 1179
HaeIII GGCC 9 cut(s) 39, 54, 58, 429, 433, 490, 504, 602, 1376
HapII CCGG 4 cut(s) 55, 324, 430, 471
HgaI GACGC 1 cut(s) 1387
Hin1II CATG 4 cut(s) 322, 374, 795, 1249
HpaII CCGG 4 cut(s) 55, 324, 430, 471
HphI GGTGA 1 cut(s) 1144
Hpy166II GTNNAC 5 cut(s) 401, 574, 592, 730, 960
Hpy188I TCNGA 3 cut(s) 847, 853, 1081
Hpy8I GTNNAC 5 cut(s) 401, 574, 592, 730, 960
Hpy99I CGWCG 2 cut(s) 1026, 1403
HpyAV CCTTC 3 cut(s) 880, 1256, 1271
HpyCH4III ACNGT 2 cut(s) 578, 1288
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 7 cut(s) 272, 281, 467, 725, 737, 866, 1430
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 8 cut(s) 29, 94, 177, 614, 857, 892, 1040, 1347
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 4 cut(s) 322, 374, 795, 1249
KroI GCCGGC 2 cut(s) 54, 429
KroNI GCCGGC 2 cut(s) 56, 431
Kzo9I GATC 3 cut(s) 224, 759, 1353
Lsp1109I GCAGC 3 cut(s) 226, 284, 991
LweI GCATC 4 cut(s) 1239, 1355, 1417, 1439
MaeI CTAG 6 cut(s) 143, 183, 719, 773, 837, 948
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 3 cut(s) 415, 901, 1132
MalI GATC 3 cut(s) 226, 761, 1355
MboI GATC 3 cut(s) 224, 759, 1353
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 5 cut(s) 95, 108, 492, 526, 1041
MluCI AATT 8 cut(s) 16, 265, 349, 511, 660, 738, 878, 942
MlyI GAGTC 5 cut(s) 73, 560, 935, 1129, 1311
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 1432
MroNI GCCGGC 2 cut(s) 54, 429
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 7 cut(s) 338, 383, 639, 659, 1097, 1110, 1272
MslI CAYNNNNRTG 1 cut(s) 1166
MspI CCGG 4 cut(s) 55, 324, 430, 471
MspR9I CCNGG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 278
NaeI GCCGGC 2 cut(s) 56, 431
NciI CCSGG 1 cut(s) 324
NdeII GATC 3 cut(s) 224, 759, 1353
NgoMIV GCCGGC 2 cut(s) 54, 429
NlaIII CATG 4 cut(s) 322, 374, 795, 1249
NlaIV GGNNCC 6 cut(s) 7, 301, 378, 490, 523, 580
NmuCI GTSAC 2 cut(s) 901, 1132
NsiI ATGCAT 1 cut(s) 1432
NspI RCATGY 1 cut(s) 322
PaeI GCATGC 1 cut(s) 322
PdiI GCCGGC 2 cut(s) 56, 431
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 7 cut(s) 12, 676, 848, 995, 1151, 1171, 1225
PflMI CCANNNNNTGG 2 cut(s) 407, 440
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 3 cut(s) 241, 274, 1006
PleI GAGTC 5 cut(s) 73, 559, 934, 1129, 1310
PpsI GAGTC 5 cut(s) 73, 559, 934, 1129, 1310
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 299
PspN4I GGNNCC 6 cut(s) 7, 301, 378, 490, 523, 580
PspOMI GGGCCC 1 cut(s) 488
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 1 cut(s) 224
RigI GGCCGGCC 2 cut(s) 58, 433
RsaI GTAC 2 cut(s) 573, 1268
RsaNI GTAC 2 cut(s) 572, 1267
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 1 cut(s) 95
SaqAI TTAA 7 cut(s) 338, 383, 639, 659, 1097, 1110, 1272
SatI GCNGC 3 cut(s) 240, 273, 1005
Sau3AI GATC 3 cut(s) 224, 759, 1353
SchI GAGTC 5 cut(s) 73, 560, 935, 1129, 1311
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 5 cut(s) 95, 108, 492, 526, 1041
SfaNI GCATC 4 cut(s) 1239, 1355, 1417, 1439
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 322
Sse9I AATT 8 cut(s) 16, 265, 349, 511, 660, 738, 878, 942
SsiI CCGC 2 cut(s) 1243, 1252
SspMI CTAG 6 cut(s) 143, 183, 719, 773, 837, 948
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 322
TaaI ACNGT 2 cut(s) 578, 1288
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 985, 1024, 1401
TaqII GACCGA 1 cut(s) 758
TasI AATT 8 cut(s) 16, 265, 349, 511, 660, 738, 878, 942
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 7 cut(s) 12, 676, 848, 995, 1151, 1171, 1225
Tru1I TTAA 7 cut(s) 338, 383, 639, 659, 1097, 1110, 1272
Tru9I TTAA 7 cut(s) 338, 383, 639, 659, 1097, 1110, 1272
TscAI CASTG 2 cut(s) 366, 1376
TseFI GTSAC 2 cut(s) 901, 1132
TseI GCWGC 3 cut(s) 239, 272, 1004
Tsp45I GTSAC 2 cut(s) 901, 1132
TspDTI ATGAA 5 cut(s) 581, 624, 688, 1197, 1332
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 2 cut(s) 366, 1376
Van91I CCANNNNNTGG 2 cut(s) 407, 440
VpaK11BI GGWCC 8 cut(s) 194, 254, 299, 356, 398, 443, 542, 1044
XapI RAATTY 3 cut(s) 349, 511, 942
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 959
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 6 cut(s) 143, 183, 719, 773, 837, 948
Zsp2I ATGCAT 1 cut(s) 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.