Rorug02G0413900

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001653
Physical Location & Seq
Forward (+)
53001765 .. 53004810
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0413900.1

Sequence Viewer

Length: 1305 bp
ATGGACTCTATCTTTCTCTCTTGGGCTGGACTTTGTGGGCTAGCCCAAAACGGTCTACAGCTTCCCTCCGTCGCATCGCCGCTTTCTGGGTTGGAGTTTCAGTGCTCATCTTCACTCTTAAACCCTGAAAACAAATCAAAACAAACCATTTTTGAACCCCAACATTTCTTCTTCTTCTTCTTCTTCACAACCACCATGCTTTCTCGTCTCCTCCCAAAACCCTCACATTTCTCTCTCCTAACTCGAATCTCCTCAAAATTCCACCCATGTCCCCACCCCCAATTCCCCTCCCCCCTCCAAAACCCTACCTTCACCACCTTCGTCCCCAGGTTCTTCTCCGCCGGCGGCGACGGCAACAACGGCCAATCCCCGTCCGACGTCTGGAACCTTTCCAAGGAAACCGATGAGAACTTCGACCCTCTGTTCGGCGAAAGCTCCGGTGACGTCTCCAGGATAGCCGAGGCTGATTCGTGGACCAAATCGACGGGCGGCGATGAGTGGGAGACGGTGCCGGGGTTTAGGCCGTGGAGCTTGGGAGGAGAGGAAGATAAGGGCGACGATTTGTTCCAAGTCGGCGATGGCGTTCAAACTAGGACTAGTGTGGAGAGTGAGCAGGAGGATGAGGGTAAGAGGATAGAGGAGGCTAAGAGGCTTGAGGGAGAGGAGAAGGAGCTCACTGCTGTTCTTAAAGGTCCAAATCGTGCATTTGGTGACCTTGTCGCTGCGTCTGGAATCACAGATGAAATGCTTGACAGTTTGATTGCATTGAAGGATTTTCGAGGGATAAAGGGATTACCTCCACTACATGAAATAGAAGACATTCGTTATCAGAAAAATACGAGTAAATCTTCGAGAGCTGATATAGAGCGCCAGAAACAGGAGGAAGTTGCCAAAGCAAGAGTGAGACTAGTGGACGACAAGGGAAGAGCTTATGGAACTGGAAGAAGGAAATGCAGTATAGCCCGTGTCTGGATTCAACCTGGTGATGGTAATTTCGTCATCAACGATAAACAGTTTGATGTCTATTTTCCAATGCTTGATCATCGCGCTGCAATCCTTCGACCGTTCTCTGAGACAAAAACTTTGGGCCTTTGGGACATCAATTGCACCGTTCAAGGGGGTGGTCTCTCAGGTCAAGTTGGAGCAATACGATTAGGAGTTAGCAGGGCCTTGCAAAATTGGGAACCAGACTTGCGTCCGACACTCAAAGCTTCTGGTTTCCTGACTAGGGACCCACGTGCTGTGGAAAGGAAAAAACCAGGAAAAGCAAAAGCAAGGAAGAGCTTCCAATGGGTCAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

434

Amino Acids

48.37

Weight (kDa)

6.64

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 314 - 434 1.7e-44 Ribosomal protein S9/S16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 381, 447
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 55
AccII CGCG 1 cut(s) 1047
AciI CCGC 4 cut(s) 80, 339, 345, 489
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 1 cut(s) 257
AcvI CACGTG 1 cut(s) 1238
AcyI GRCGYC 2 cut(s) 378, 444
AfiI CCNNNNNNNGG 9 cut(s) 86, 381, 394, 425, 877, 969, 986, 1116, 1228
AgsI TTSAA 5 cut(s) 155, 587, 769, 977, 1115
AhlI ACTAGT 2 cut(s) 596, 907
AjnI CCWGG 4 cut(s) 326, 449, 979, 1258
AluBI AGCT 8 cut(s) 61, 435, 531, 673, 857, 929, 1211, 1284
AluI AGCT 8 cut(s) 61, 435, 531, 673, 857, 929, 1211, 1284
Alw21I GWGCWC 2 cut(s) 107, 675
Alw26I GTCTC 6 cut(s) 212, 451, 497, 898, 1067, 1130
AoxI GGCC 4 cut(s) 361, 521, 1087, 1167
ApeKI GCWGC 2 cut(s) 722, 1049
ApoI RAATTY 1 cut(s) 257
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 1066, 1098
Asp700I GAANNNNTTC 2 cut(s) 819, 1283
AspLEI GCGC 2 cut(s) 870, 1049
AspS9I GGNCC 5 cut(s) 474, 692, 1087, 1167, 1231
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 4 cut(s) 304, 452, 722, 995
AsuNHI GCTAGC 1 cut(s) 40
AvaII GGWCC 3 cut(s) 474, 692, 1231
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 675
BbrPI CACGTG 1 cut(s) 1238
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 2 cut(s) 107, 675
BbvI GCAGC 2 cut(s) 709, 1036
BccI CCATC 2 cut(s) 572, 980
BceAI ACGGC 3 cut(s) 367, 376, 508
BciT130I CCWGG 4 cut(s) 328, 451, 981, 1260
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 6 cut(s) 212, 451, 497, 898, 1067, 1130
BcuI ACTAGT 2 cut(s) 596, 907
BfaI CTAG 5 cut(s) 41, 591, 597, 908, 1227
BfmI CTRYAG 1 cut(s) 56
BfoI RGCGCY 1 cut(s) 871
BisI GCNGC 5 cut(s) 80, 346, 490, 723, 1050
BlsI GCNGC 5 cut(s) 81, 347, 491, 724, 1051
Bme1390I CCNGG 5 cut(s) 328, 451, 513, 981, 1260
Bme18I GGWCC 3 cut(s) 474, 692, 1231
BmgT120I GGNCC 5 cut(s) 474, 692, 1087, 1167, 1231
BmiI GGNNCC 5 cut(s) 386, 510, 1185, 1232, 1233
BmrFI CCNGG 5 cut(s) 328, 451, 513, 981, 1260
BmsI GCATC 1 cut(s) 83
BmtI GCTAGC 1 cut(s) 44
BoxI GACNNNNGTC 1 cut(s) 1194
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 433
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 513
BsaAI YACGTR 1 cut(s) 1238
BsaHI GRCGYC 2 cut(s) 378, 444
BsaI GGTCTC 1 cut(s) 1130
BsaJI CCNNGG 5 cut(s) 326, 393, 459, 512, 524
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 9 cut(s) 86, 381, 394, 425, 877, 969, 986, 1116, 1228
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 943
BseBI CCWGG 4 cut(s) 328, 451, 981, 1260
BseDI CCNNGG 5 cut(s) 326, 393, 459, 512, 524
BseGI GGATG 1 cut(s) 625
BseLI CCNNNNNNNGG 9 cut(s) 86, 381, 394, 425, 877, 969, 986, 1116, 1228
BseMII CTCAG 2 cut(s) 1062, 1143
BseNI ACTGG 1 cut(s) 943
BseRI GAGGAG 5 cut(s) 200, 241, 552, 653, 677
BseXI GCAGC 2 cut(s) 709, 1036
Bsh1236I CGCG 1 cut(s) 1047
Bsh1285I CGRYCG 1 cut(s) 1064
BshFI GGCC 4 cut(s) 363, 523, 1089, 1169
BshNI GGYRCC 1 cut(s) 508
BsiEI CGRYCG 1 cut(s) 1064
BsiHKAI GWGCWC 2 cut(s) 107, 675
BsiSI CCGG 3 cut(s) 342, 438, 512
BslFI GGGAC 4 cut(s) 255, 308, 1109, 1244
BslI CCNNNNNNNGG 9 cut(s) 86, 381, 394, 425, 877, 969, 986, 1116, 1228
BsmAI GTCTC 6 cut(s) 212, 451, 497, 898, 1067, 1130
BsmBI CGTCTC 3 cut(s) 212, 451, 497
BsmFI GGGAC 4 cut(s) 255, 308, 1109, 1244
BsnI GGCC 4 cut(s) 363, 523, 1089, 1169
Bso31I GGTCTC 1 cut(s) 1130
Bsp1286I GDGCHC 2 cut(s) 107, 675
Bsp143I GATC 1 cut(s) 1039
BspACI CCGC 4 cut(s) 80, 339, 345, 489
BspANI GGCC 4 cut(s) 363, 523, 1089, 1169
BspCNI CTCAG 2 cut(s) 1063, 1142
BspFNI CGCG 1 cut(s) 1047
BspLI GGNNCC 5 cut(s) 386, 510, 1185, 1232, 1233
BspOI GCTAGC 1 cut(s) 44
BspQI GCTCTTC 2 cut(s) 919, 1274
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 1130
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 943
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 5 cut(s) 326, 393, 459, 512, 524
BssMI GATC 1 cut(s) 1039
BssNI GRCGYC 2 cut(s) 378, 444
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 4 cut(s) 328, 451, 981, 1260
Bst4CI ACNGT 6 cut(s) 53, 508, 755, 1014, 1065, 1111
Bst6I CTCTTC 2 cut(s) 919, 1274
BstACI GRCGYC 2 cut(s) 378, 444
BstBAI YACGTR 1 cut(s) 1238
BstC8I GCNNGC 2 cut(s) 42, 343
BstDEI CTNAG 3 cut(s) 645, 1071, 1129
BstDSI CCRYGG 1 cut(s) 524
BstEII GGTNACC 1 cut(s) 710
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 1 cut(s) 1047
BstH2I RGCGCY 1 cut(s) 871
BstHHI GCGC 2 cut(s) 870, 1049
BstKTI GATC 1 cut(s) 1042
BstMAI GTCTC 6 cut(s) 212, 451, 497, 898, 1067, 1130
BstMBI GATC 1 cut(s) 1039
BstMCI CGRYCG 1 cut(s) 1064
BstMWI GCNNNNNNNGC 2 cut(s) 351, 360
BstNI CCWGG 4 cut(s) 328, 451, 981, 1260
BstPAI GACNNNNGTC 1 cut(s) 1194
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 5 cut(s) 326, 449, 511, 979, 1258
BstSFI CTRYAG 1 cut(s) 56
BstUI CGCG 1 cut(s) 1047
BstV1I GCAGC 2 cut(s) 709, 1036
BstV2I GAAGAC 1 cut(s) 822
BsuRI GGCC 4 cut(s) 363, 523, 1089, 1169
BtgI CCRYGG 1 cut(s) 524
BtgZI GCGATG 4 cut(s) 60, 507, 591, 1028
BtsCI GGATG 1 cut(s) 625
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 2 cut(s) 107, 675
Cac8I GCNNGC 2 cut(s) 42, 343
CfoI GCGC 2 cut(s) 870, 1049
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 5 cut(s) 474, 692, 1087, 1167, 1231
CseI GACGC 2 cut(s) 714, 1184
CsiI ACCWGGT 1 cut(s) 979
CviAII CATG 3 cut(s) 196, 267, 806
DdeI CTNAG 3 cut(s) 645, 1071, 1129
DpnI GATC 1 cut(s) 1041
DpnII GATC 1 cut(s) 1039
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 919, 1274
EarI CTCTTC 2 cut(s) 919, 1274
EciI GGCGGA 1 cut(s) 328
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 1130
Eco47I GGWCC 3 cut(s) 474, 692, 1231
Eco53kI GAGCTC 1 cut(s) 673
Eco72I CACGTG 1 cut(s) 1238
Eco91I GGTNACC 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 673
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoO109I RGGNCCY 2 cut(s) 1167, 1231
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 4 cut(s) 326, 449, 979, 1258
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 3 cut(s) 212, 451, 497
FaeI CATG 3 cut(s) 199, 270, 809
FaiI YATR 6 cut(s) 197, 268, 807, 863, 933, 959
FaqI GGGAC 4 cut(s) 255, 308, 1109, 1244
FatI CATG 3 cut(s) 195, 266, 805
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 5 cut(s) 80, 346, 490, 723, 1050
FokI GGATG 1 cut(s) 632
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 5 cut(s) 80, 346, 490, 723, 1050
FspBI CTAG 5 cut(s) 41, 591, 597, 908, 1227
GlaI GCGC 2 cut(s) 869, 1048
GluI GCNGC 5 cut(s) 80, 346, 490, 723, 1050
GsuI CTGGAG 1 cut(s) 433
HaeII RGCGCY 1 cut(s) 871
HaeIII GGCC 4 cut(s) 363, 523, 1089, 1169
HapII CCGG 3 cut(s) 342, 438, 512
HgaI GACGC 2 cut(s) 714, 1184
HhaI GCGC 2 cut(s) 870, 1049
Hin1I GRCGYC 2 cut(s) 378, 444
Hin1II CATG 3 cut(s) 199, 270, 809
Hin6I GCGC 2 cut(s) 868, 1047
HinP1I GCGC 2 cut(s) 868, 1047
HindIII AAGCTT 1 cut(s) 1209
HinfI GANTC 5 cut(s) 5, 246, 467, 732, 973
HpaII CCGG 3 cut(s) 342, 438, 512
HphI GGTGA 4 cut(s) 304, 452, 722, 995
Hpy166II GTNNAC 3 cut(s) 56, 474, 913
Hpy188I TCNGA 4 cut(s) 376, 831, 1072, 1200
Hpy188III TCNNGA 5 cut(s) 382, 729, 852, 970, 1222
Hpy8I GTNNAC 3 cut(s) 56, 474, 913
Hpy99I CGWCG 5 cut(s) 74, 353, 380, 487, 560
HpyAV CCTTC 6 cut(s) 319, 328, 661, 763, 939, 1067
HpyCH4III ACNGT 6 cut(s) 53, 508, 755, 1014, 1065, 1111
HpyCH4IV ACGT 3 cut(s) 378, 444, 1237
HpyCH4V TGCA 6 cut(s) 704, 764, 954, 1052, 1107, 1174
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 360
HpyF3I CTNAG 3 cut(s) 645, 1071, 1129
HpySE526I ACGT 3 cut(s) 378, 444, 1237
Hsp92I GRCGYC 2 cut(s) 378, 444
Hsp92II CATG 3 cut(s) 199, 270, 809
HspAI GCGC 2 cut(s) 868, 1047
KflI GGGWCCC 1 cut(s) 1231
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 1 cut(s) 1039
LguI GCTCTTC 2 cut(s) 919, 1274
LmnI GCTCC 4 cut(s) 440, 528, 670, 1142
Lsp1109I GCAGC 2 cut(s) 709, 1036
LweI GCATC 1 cut(s) 83
MabI ACCWGGT 1 cut(s) 979
MaeI CTAG 5 cut(s) 41, 591, 597, 908, 1227
MaeII ACGT 3 cut(s) 378, 444, 1237
MaeIII GTNAC 2 cut(s) 440, 710
MalI GATC 1 cut(s) 1041
MboI GATC 1 cut(s) 1039
MfeI CAATTG 1 cut(s) 1102
MhlI GDGCHC 2 cut(s) 107, 675
MluCI AATT 5 cut(s) 257, 281, 991, 1102, 1177
MmeI TCCRAC 4 cut(s) 72, 399, 1120, 1223
MreI CGCCGGCG 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 341
MroXI GAANNNNTTC 2 cut(s) 819, 1283
MseI TTAA 2 cut(s) 119, 687
MspI CCGG 3 cut(s) 342, 438, 512
MspR9I CCNGG 5 cut(s) 328, 451, 513, 981, 1260
MunI CAATTG 1 cut(s) 1102
MvaI CCWGG 4 cut(s) 328, 451, 981, 1260
MvnI CGCG 1 cut(s) 1047
MwoI GCNNNNNNNGC 2 cut(s) 351, 360
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 513
NdeII GATC 1 cut(s) 1039
NgoMIV GCCGGC 1 cut(s) 341
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 199, 270, 809
NlaIV GGNNCC 5 cut(s) 386, 510, 1185, 1232, 1233
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 440, 710
PciSI GCTCTTC 2 cut(s) 919, 1274
PcsI WCGNNNNNNNCGW 2 cut(s) 579, 1002
PdiI GCCGGC 1 cut(s) 343
PdmI GAANNNNTTC 2 cut(s) 819, 1283
PfeI GAWTC 4 cut(s) 246, 467, 732, 973
PflFI GACNNNGTC 1 cut(s) 716
PfoI TCCNGGA 1 cut(s) 449
PkrI GCNGC 5 cut(s) 81, 347, 491, 724, 1051
PmaCI CACGTG 1 cut(s) 1238
PmlI CACGTG 1 cut(s) 1238
Ppu21I YACGTR 1 cut(s) 1238
PpuMI RGGWCCY 1 cut(s) 1231
PshAI GACNNNNGTC 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 1231
Psp6I CCWGG 4 cut(s) 326, 449, 979, 1258
PspCI CACGTG 1 cut(s) 1238
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 4 cut(s) 326, 449, 979, 1258
PspN4I GGNNCC 5 cut(s) 386, 510, 1185, 1232, 1233
PspPI GGNCC 5 cut(s) 474, 692, 1087, 1167, 1231
PspPPI RGGWCCY 1 cut(s) 1231
PsyI GACNNNGTC 1 cut(s) 716
SacI GAGCTC 1 cut(s) 675
SapI GCTCTTC 2 cut(s) 919, 1274
SaqAI TTAA 2 cut(s) 119, 687
SatI GCNGC 5 cut(s) 80, 346, 490, 723, 1050
Sau3AI GATC 1 cut(s) 1039
Sau96I GGNCC 5 cut(s) 474, 692, 1087, 1167, 1231
ScrFI CCNGG 5 cut(s) 328, 451, 513, 981, 1260
SduI GDGCHC 2 cut(s) 107, 675
SexAI ACCWGGT 1 cut(s) 979
SfaNI GCATC 1 cut(s) 83
SfcI CTRYAG 1 cut(s) 56
SgrAI CRCCGGYG 1 cut(s) 341
SinI GGWCC 3 cut(s) 474, 692, 1231
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SpeI ACTAGT 2 cut(s) 596, 907
Sse9I AATT 5 cut(s) 257, 281, 991, 1102, 1177
SsiI CCGC 4 cut(s) 80, 339, 345, 489
SspMI CTAG 5 cut(s) 41, 591, 597, 908, 1227
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 5 cut(s) 326, 449, 511, 979, 1258
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 6 cut(s) 53, 508, 755, 1014, 1065, 1111
TaiI ACGT 3 cut(s) 381, 447, 1240
TaqI TCGA 6 cut(s) 244, 414, 482, 778, 851, 1060
TasI AATT 5 cut(s) 257, 281, 991, 1102, 1177
TauI GCSGC 3 cut(s) 82, 348, 492
TfiI GAWTC 4 cut(s) 246, 467, 732, 973
Tru1I TTAA 2 cut(s) 119, 687
Tru9I TTAA 2 cut(s) 119, 687
TscAI CASTG 2 cut(s) 107, 682
TseFI GTSAC 2 cut(s) 440, 710
TseI GCWGC 2 cut(s) 722, 1049
Tsp45I GTSAC 2 cut(s) 440, 710
TspDTI ATGAA 2 cut(s) 756, 822
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 2 cut(s) 107, 682
Tth111I GACNNNGTC 1 cut(s) 716
VpaK11BI GGWCC 3 cut(s) 474, 692, 1231
XagI CCTNNNNNAGG 1 cut(s) 392
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 55
XmnI GAANNNNTTC 2 cut(s) 819, 1283
XspI CTAG 5 cut(s) 41, 591, 597, 908, 1227
ZraI GACGTC 2 cut(s) 379, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.