Rorug02G0414100

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001653
Physical Location & Seq
Reverse (-)
53009770 .. 53015128
5359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0414100.1

Sequence Viewer

Length: 1512 bp
ATGTACAGAACTGCAGCTTCGCGAGTCAGGTCGCTTAAGGGCCGTGTGGGCACCACCAGGTATGCGTCGGTAACTGCAGCTGCTACAAAGCCAGCCTCAGGGGGTATCTTTAGCTGGCTGACCGGGGAAAAGTCCAGTGCGCTTCCTCGTCTGGAAACCCCATTAGCAGGTGTGAACCTCCCTCCTCCACTACCTGATTACGTGGAACCGAGCAAGACTAAGGTTACAAAGCTCTCCAATGGTGTCAGAATAGCTTCAGAAAAGTCGCCGAGTCCTGCAGCCTCCATTGGATTATATGTTAATTGTGGTTCCATTTATGAGACTCCAGTAACGAGTGGGGCTTCACACTTGTTAGAAAGGCTGGCCTTTAAGAGTACAACCAACCGGAGCCAATTGCGCATTGTTAGAGAAGTGGAAGCAATTGGTGGTAACATTGGAGCCAGTGCCTCTAGGGAACAAATGGGATACACTTTCGATGCTCTTAAAACCTATGTTCCTCAGATGGTGGAATTGCTTGTTGACTCTGTGAGAAATCCTGCCTTCTTGGAATGGGAGGTCAAAGAGGAGCTGGAAAAGGTGCGGGCAGAAATTGCTGACCTTTCTAAAAATCCTCAAGGCTTACTCTTGGAGAGGATTCATTCGGCTGGTTTTTCTGGAGCTTTGAGAAATCCTCTTTTGGCTTCTGAAGACGCACTGAACAGATTGGATAGCAGCATCCTTGAGGAATTTGTTGCTGAGAATTATACTGCTCCTCGGATAGTTCTTGCAGCATCTGGGGTTGACCATGAGGAGCTTTTATCTATTGCAGAACCACTTCTCTCTGACCTACCAAGTGTGCCCCTTGTTGGCGAGCCAAAATCTCAATATGTTGGAGGGGAGTATCGTACTCAAGGTGATACAAAGGATGAGGCACATGTTGCTCTTGCTTTTGAAGTTCCTGGTGGTTGGCGTCAAGAGAAGCAAGCTATCATTTTGACTGTTCTCCAGTTGCTTATGGGAGGAGGTGGCTCTTTCTCTGCCGGGGGTCCTGGAAAGGGGATGCACTCAAGGCTATATCTCCGTGTCTTGAATAATCATCAGGAGATTCAAAATTTTACTGCATTCAACAGCCTCTTTAATGATTCTGGATTGTTTGGAATTTATGCTAGCACTGGCTCTGGGTTTGCATCACAGGCAGTTGAAATAGCAGCAAAAGAGTTACTTTCAATTGCCACACCTGGACAAGTTTCGGATGTAGAGCTTAAACGTGCTAAAGAGTCCACAAAGGCTGCAGTTTTGATGAATCTAGAATCTAGAATGATTGCATCAGAAGATATAGGAAGGCAAGTTCTGACATATGGCGAGAGGAAACCTGTGGAGGATTTCTTAACGACAGTAGAAGGAGTGACTTTGAAAGATATTACTACAGCTGCCCAACAGATTATATCTTCACCTCTAACAATGGCATCATATGGGAATGTTGTAAATGTCCCAAGCTACGATTCAGTTAACAGAATCTTCCATGCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

503

Amino Acids

54.1

Weight (kDa)

5.94

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 83 - 227 9e-42 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 240 - 419 1.3e-31 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 158
Acc16I TGCGCA 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 50
AccII CGCG 1 cut(s) 22
AciI CCGC 1 cut(s) 580
AcsI RAATTY 3 cut(s) 725, 1090, 1137
AcuI CTGAAG 2 cut(s) 240, 705
AcyI GRCGYC 1 cut(s) 949
AfaI GTAC 3 cut(s) 5, 376, 886
AfiI CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
AflII CTTAAG 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 913
AgsI TTSAA 7 cut(s) 932, 1069, 1088, 1105, 1181, 1206, 1393
AjnI CCWGG 4 cut(s) 56, 937, 1027, 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 1465, 1497
AloI GAACNNNNNNTCC 2 cut(s) 1311, 1343
Alw26I GTCTC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 773
AoxI GGCC 2 cut(s) 40, 363
ApeKI GCWGC 9 cut(s) 14, 77, 80, 278, 711, 767, 1187, 1268, 1409
ApoI RAATTY 3 cut(s) 725, 1090, 1137
Asp700I GAANNNNTTC 2 cut(s) 253, 813
AspLEI GCGC 2 cut(s) 142, 399
AspS9I GGNCC 2 cut(s) 40, 1025
AsuC2I CCSGG 2 cut(s) 124, 1021
AsuHPI GGTGA 2 cut(s) 905, 1422
AsuNHI GCTAGC 1 cut(s) 1145
AvaII GGWCC 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 97
BaeGI GKGCMC 2 cut(s) 53, 840
BanI GGYRCC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 693
BbvI GCAGC 9 cut(s) 26, 67, 89, 290, 723, 779, 1199, 1255, 1396
BccI CCATC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 4 cut(s) 58, 939, 1029, 1218
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 2 cut(s) 124, 1021
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 4 cut(s) 450, 1146, 1286, 1293
BfmI CTRYAG 5 cut(s) 12, 75, 276, 1269, 1404
BfrI CTTAAG 1 cut(s) 35
BfuAI ACCTGC 1 cut(s) 158
BfuI GTATCC 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 48
BisI GCNGC 9 cut(s) 15, 78, 81, 279, 712, 768, 1188, 1269, 1410
BlsI GCNGC 9 cut(s) 16, 79, 82, 280, 713, 769, 1189, 1270, 1411
Bme1390I CCNGG 6 cut(s) 58, 124, 939, 1021, 1029, 1218
Bme18I GGWCC 1 cut(s) 1025
BmgT120I GGNCC 2 cut(s) 40, 1025
BmiI GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
BmrFI CCNGG 6 cut(s) 58, 124, 939, 1021, 1029, 1218
BmsI GCATC 7 cut(s) 466, 723, 779, 1029, 1175, 1313, 1454
BmtI GCTAGC 1 cut(s) 1149
BpiI GAAGAC 1 cut(s) 693
BplI GAGNNNNNCTC 2 cut(s) 655, 687
BpmI CTGGAG 3 cut(s) 309, 675, 968
BpuEI CTTGAG 4 cut(s) 597, 740, 873, 1030
BpuMI CCSGG 2 cut(s) 124, 1021
BsaAI YACGTR 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 949
BsaJI CCNNGG 3 cut(s) 123, 752, 1020
BsaWI WCCGGW 1 cut(s) 384
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
Bse1I ACTGG 5 cut(s) 135, 326, 441, 985, 1156
Bse21I CCTNAGG 1 cut(s) 97
BseBI CCWGG 4 cut(s) 58, 939, 1029, 1218
BseDI CCNNGG 3 cut(s) 123, 752, 1020
BseGI GGATG 4 cut(s) 714, 910, 1044, 1237
BseLI CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
BseMII CTCAG 3 cut(s) 111, 512, 726
BseNI ACTGG 5 cut(s) 135, 326, 441, 985, 1156
BseRI GAGGAG 5 cut(s) 174, 578, 741, 803, 1014
BseSI GKGCMC 2 cut(s) 53, 840
BseXI GCAGC 9 cut(s) 26, 67, 89, 290, 723, 779, 1199, 1255, 1396
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 42, 365
BshNI GGYRCC 1 cut(s) 50
BsiSI CCGG 3 cut(s) 123, 385, 1020
BslFI GGGAC 1 cut(s) 1454
BslI CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 1454
BsmI GAATGC 1 cut(s) 1100
BsnI GGCC 2 cut(s) 42, 365
Bsp1286I GDGCHC 2 cut(s) 53, 840
Bsp1407I TGTACA 1 cut(s) 3
Bsp68I TCGCGA 1 cut(s) 22
BspACI CCGC 1 cut(s) 580
BspANI GGCC 2 cut(s) 42, 365
BspCNI CTCAG 3 cut(s) 110, 511, 727
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
BspMAI CTGCAG 4 cut(s) 16, 79, 280, 1273
BspMI ACCTGC 1 cut(s) 158
BspOI GCTAGC 1 cut(s) 1149
BspT107I GGYRCC 1 cut(s) 50
BspTI CTTAAG 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 135, 326, 441, 985, 1156
BssECI CCNNGG 3 cut(s) 123, 752, 1020
BssNI GRCGYC 1 cut(s) 949
Bst2UI CCWGG 4 cut(s) 58, 939, 1029, 1218
Bst4CI ACNGT 2 cut(s) 979, 1375
BstACI GRCGYC 1 cut(s) 949
BstAFI CTTAAG 1 cut(s) 35
BstAPI GCANNNNNTGC 2 cut(s) 590, 917
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 202
BstC8I GCNNGC 7 cut(s) 93, 116, 363, 582, 851, 963, 1147
BstDEI CTNAG 4 cut(s) 97, 219, 498, 735
BstF5I GGATG 4 cut(s) 714, 910, 1044, 1237
BstFNI CGCG 1 cut(s) 22
BstHHI GCGC 2 cut(s) 142, 399
BstMAI GTCTC 1 cut(s) 314
BstMWI GCNNNNNNNGC 6 cut(s) 48, 396, 590, 917, 1048, 1172
BstNI CCWGG 4 cut(s) 58, 939, 1029, 1218
BstNSI RCATGY 1 cut(s) 917
BstSCI CCNGG 6 cut(s) 56, 122, 937, 1019, 1027, 1216
BstSFI CTRYAG 5 cut(s) 12, 75, 276, 1269, 1404
BstSLI GKGCMC 2 cut(s) 53, 840
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 9 cut(s) 26, 67, 89, 290, 723, 779, 1199, 1255, 1396
BstV2I GAAGAC 1 cut(s) 693
Bsu36I CCTNAGG 1 cut(s) 97
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 42, 365
BtsCI GGATG 4 cut(s) 714, 910, 1044, 1237
BtsIMutI CAGTG 4 cut(s) 142, 448, 692, 1149
BtuMI TCGCGA 1 cut(s) 22
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 7 cut(s) 93, 116, 363, 582, 851, 963, 1147
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 2 cut(s) 142, 399
Cfr13I GGNCC 2 cut(s) 40, 1025
CseI GACGC 3 cut(s) 54, 698, 938
CsiI ACCWGGT 1 cut(s) 56
Csp6I GTAC 3 cut(s) 4, 375, 885
CviAII CATG 3 cut(s) 785, 914, 1502
CviQI GTAC 3 cut(s) 4, 375, 885
DdeI CTNAG 4 cut(s) 97, 219, 498, 735
Eco47I GGWCC 1 cut(s) 1025
Eco57I CTGAAG 2 cut(s) 240, 705
Eco81I CCTNAGG 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 1025
EcoRII CCWGG 4 cut(s) 56, 937, 1027, 1216
FaeI CATG 3 cut(s) 788, 917, 1505
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 1 cut(s) 1454
FatI CATG 3 cut(s) 784, 913, 1501
FauI CCCGC 1 cut(s) 573
FauNDI CATATG 2 cut(s) 1336, 1450
Fnu4HI GCNGC 9 cut(s) 15, 78, 81, 279, 712, 768, 1188, 1269, 1410
FokI GGATG 4 cut(s) 701, 917, 1051, 1244
Fsp4HI GCNGC 9 cut(s) 15, 78, 81, 279, 712, 768, 1188, 1269, 1410
FspBI CTAG 4 cut(s) 450, 1146, 1286, 1293
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 2 cut(s) 141, 398
GluI GCNGC 9 cut(s) 15, 78, 81, 279, 712, 768, 1188, 1269, 1410
GsuI CTGGAG 3 cut(s) 309, 675, 968
HaeIII GGCC 2 cut(s) 42, 365
HapII CCGG 3 cut(s) 123, 385, 1020
HgaI GACGC 3 cut(s) 54, 698, 938
HhaI GCGC 2 cut(s) 142, 399
Hin1I GRCGYC 1 cut(s) 949
Hin1II CATG 3 cut(s) 788, 917, 1505
Hin6I GCGC 2 cut(s) 140, 397
HinP1I GCGC 2 cut(s) 140, 397
HincII GTYRAC 3 cut(s) 520, 781, 1489
HindII GTYRAC 3 cut(s) 520, 781, 1489
HpaI GTTAAC 1 cut(s) 1489
HpaII CCGG 3 cut(s) 123, 385, 1020
HphI GGTGA 2 cut(s) 905, 1422
Hpy166II GTNNAC 5 cut(s) 175, 520, 781, 1260, 1489
Hpy188I TCNGA 9 cut(s) 248, 259, 501, 685, 756, 823, 1231, 1309, 1332
Hpy188III TCNNGA 9 cut(s) 21, 152, 654, 953, 1066, 1079, 1125, 1286, 1293
Hpy8I GTNNAC 5 cut(s) 175, 520, 781, 1260, 1489
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 3 cut(s) 550, 1314, 1373
HpyCH4III ACNGT 2 cut(s) 979, 1375
HpyCH4IV ACGT 2 cut(s) 201, 1246
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 396, 590, 917, 1048, 1172
HpyF3I CTNAG 4 cut(s) 97, 219, 498, 735
HpySE526I ACGT 2 cut(s) 201, 1246
Hsp92I GRCGYC 1 cut(s) 949
Hsp92II CATG 3 cut(s) 788, 917, 1505
HspAI GCGC 2 cut(s) 140, 397
KspAI GTTAAC 1 cut(s) 1489
LmnI GCTCC 6 cut(s) 387, 437, 565, 656, 754, 790
Lsp1109I GCAGC 9 cut(s) 26, 67, 89, 290, 723, 779, 1199, 1255, 1396
LweI GCATC 7 cut(s) 466, 723, 779, 1029, 1175, 1313, 1454
MabI ACCWGGT 1 cut(s) 56
MaeI CTAG 4 cut(s) 450, 1146, 1286, 1293
MaeII ACGT 2 cut(s) 201, 1246
MaeIII GTNAC 6 cut(s) 70, 223, 328, 428, 1197, 1384
MboII GAAGA 4 cut(s) 698, 1322, 1419, 1489
MfeI CAATTG 3 cut(s) 392, 420, 1206
MhlI GDGCHC 2 cut(s) 53, 840
MlyI GAGTC 5 cut(s) 33, 280, 316, 515, 1265
MmeI TCCRAC 1 cut(s) 850
MroXI GAANNNNTTC 2 cut(s) 253, 813
MseI TTAA 8 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1488
MspA1I CMGCKG 2 cut(s) 80, 1409
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 3 cut(s) 123, 385, 1020
MspR9I CCNGG 6 cut(s) 58, 124, 939, 1021, 1029, 1218
MunI CAATTG 3 cut(s) 392, 420, 1206
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 4 cut(s) 58, 939, 1029, 1218
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 6 cut(s) 48, 396, 590, 917, 1048, 1172
NciI CCSGG 2 cut(s) 124, 1021
NdeI CATATG 2 cut(s) 1336, 1450
NheI GCTAGC 1 cut(s) 1145
NlaIII CATG 3 cut(s) 788, 917, 1505
NlaIV GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 1384
NruI TCGCGA 1 cut(s) 22
NsbI TGCGCA 1 cut(s) 398
NspI RCATGY 1 cut(s) 917
PaqCI CACCTGC 1 cut(s) 158
PciI ACATGT 1 cut(s) 913
PctI GAATGC 1 cut(s) 1100
PdmI GAANNNNTTC 2 cut(s) 253, 813
PfeI GAWTC 7 cut(s) 634, 1084, 1121, 1282, 1289, 1481, 1494
PfoI TCCNGGA 1 cut(s) 1027
PkrI GCNGC 9 cut(s) 16, 79, 82, 280, 713, 769, 1189, 1270, 1411
PleI GAGTC 5 cut(s) 32, 279, 316, 515, 1264
PpsI GAGTC 5 cut(s) 32, 279, 316, 515, 1264
Ppu21I YACGTR 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 1025
PscI ACATGT 1 cut(s) 913
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 4 cut(s) 56, 937, 1027, 1216
PspGI CCWGG 4 cut(s) 56, 937, 1027, 1216
PspN4I GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
PspPI GGNCC 2 cut(s) 40, 1025
PspPPI RGGWCCY 1 cut(s) 1025
PstI CTGCAG 4 cut(s) 16, 79, 280, 1273
PstNI CAGNNNCTG 1 cut(s) 773
PvuII CAGCTG 2 cut(s) 80, 1409
RruI TCGCGA 1 cut(s) 22
RsaI GTAC 3 cut(s) 5, 376, 886
RsaNI GTAC 3 cut(s) 4, 375, 885
SaqAI TTAA 8 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1488
SatI GCNGC 9 cut(s) 15, 78, 81, 279, 712, 768, 1188, 1269, 1410
Sau96I GGNCC 2 cut(s) 40, 1025
SchI GAGTC 5 cut(s) 33, 280, 316, 515, 1265
ScrFI CCNGG 6 cut(s) 58, 124, 939, 1021, 1029, 1218
SduI GDGCHC 2 cut(s) 53, 840
SexAI ACCWGGT 1 cut(s) 56
SfaNI GCATC 7 cut(s) 466, 723, 779, 1029, 1175, 1313, 1454
SfcI CTRYAG 5 cut(s) 12, 75, 276, 1269, 1404
SinI GGWCC 1 cut(s) 1025
SmlI CTYRAG 5 cut(s) 35, 612, 719, 888, 1045
SmoI CTYRAG 5 cut(s) 35, 612, 719, 888, 1045
SsiI CCGC 1 cut(s) 580
SspMI CTAG 4 cut(s) 450, 1146, 1286, 1293
StyD4I CCNGG 6 cut(s) 56, 122, 937, 1019, 1027, 1216
TaaI ACNGT 2 cut(s) 979, 1375
TaiI ACGT 2 cut(s) 204, 1249
TaqI TCGA 1 cut(s) 474
TatI WGTACW 2 cut(s) 3, 374
TfiI GAWTC 7 cut(s) 634, 1084, 1121, 1282, 1289, 1481, 1494
Tru1I TTAA 8 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1488
Tru9I TTAA 8 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1488
TscAI CASTG 4 cut(s) 142, 448, 699, 1156
TseFI GTSAC 1 cut(s) 1384
TseI GCWGC 9 cut(s) 14, 77, 80, 278, 711, 767, 1187, 1268, 1409
Tsp45I GTSAC 1 cut(s) 1384
TspDTI ATGAA 2 cut(s) 626, 1295
TspGWI ACGGA 1 cut(s) 1049
TspRI CASTG 4 cut(s) 142, 448, 699, 1156
Vha464I CTTAAG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 1025
XapI RAATTY 3 cut(s) 725, 1090, 1137
XbaI TCTAGA 2 cut(s) 1285, 1292
XceI RCATGY 1 cut(s) 917
XmnI GAANNNNTTC 2 cut(s) 253, 813
XspI CTAG 4 cut(s) 450, 1146, 1286, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.