Prupe.3G020800_v2.0.a1

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001653
Physical Location & Seq
Reverse (-)
1514608 .. 1519469
4862 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G020800.2

Sequence Viewer

Length: 1458 bp
ATGGGTTCCAGATTCCCATCTCATCAGCTCAGCAATGGCCTTTACGTATCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCCATGCCCTATACTGGTGGAGATATCAAGAAGTCGGGAGAACTAGGAAAGATGTTTGATATCCCTATGGATGGCTCTAAGTCTAGAAAATCTGGACCTATAACTGGTCCTCCTTCAAGGACTGGATCTTTTGGAGGTGCTGCTTCACATTCAGGACCAATCCCTAATGCTGCAGTTCGTGCTGGCTATACCACATCAGGTCCCGTATCATCTGGAGGCATGCCTGGCCCAGCTTCCTTAAAGAAGTCAAATTCTGGACCGCTAAATAAGCATGGGGAACCTCTTAAGAAGTCGTCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCTGGACCTCTTCCTCCTGTTCTCCCTGCAACAGGTCTCATTACATCTGGACCCATTTCTTCTGGCCCACTAAATGCTTCTGGGGCCCCTCGTAAGGTATCCGGTCCTTTGGAGTCAATGGGATCAATGAAAGTACAAGGTTCCTCCATTGTTCACAATCAGGCCATTACTACTCTTAGCCAAGATGATGAGTTTTCATTTAGGAAGAGCTTCCCAAAGCTAATATTATGGTCTTTGATTCTACTTTTCGTGATGGGGTTCATAGCTGGTGGTTTTATTCTAGGTGCAGTCCACAATGCATATCTCCTCATTGTGGTTGTGATCCTTTTTGGTGCAGTTGCTACACTATTCACATGGAATACTTATTGGGGAAGACGAGCTATTATAGGTTATATTGCTAGCTATTCAGATTCTGAGCTGAGGACTGCGAAGAATGGGCAATTTGTGAAGGTCTCAGGGGTGGTCACTTGTGGCAATCTGCCCCTTGAGTCGTCCTTTCAAAAGGTTCCTAGATGTGTGTATACATCTACAAGTTTGTACGAATATCGAGGATGGGATTCAAAAGCTGCCAACCCTACACATCGTCGCTTTACTTGGGGGCTCAGGTCCATAGAAGGCCGTGTTGTAGATTTCTACATCTCTGATTTCCAATCCGGGTTAAGAGCATTGGTTAAGACTGGCTCTGGAGCAAGGGTGACTCCTTATGTTGATGATTCGATTGTCATTGATGTGAACCCTGAAAATGAAGAGTTGTCTCCAGAGTTTATTAGGTGGTTGGGAGAGAGGAACCTTTCAAGTGATGATCGCATGATGCGGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTTAGTGTAATGGGAGTTGTCCAGAGAAATGACAATGTGCTGATGATCGTTCCTCCCCCTGAGCCGATCACGACAGGATGCCAGTGGGCCAAATGTATCTTTCCAGCTAGCCTTGAGGGTATTGTTTTGAGATGTGATGATGCATCAAAGAATGATGTCATACCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

486

Amino Acids

51.85

Weight (kDa)

9.46

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 959
AciI CCGC 3 cut(s) 359, 430, 1252
AclWI GGATC 3 cut(s) 232, 568, 754
AcoI YGGCCR 2 cut(s) 56, 427
AcsI RAATTY 1 cut(s) 349
AfaI GTAC 3 cut(s) 573, 977, 1268
AfiI CCNNNNNNNGG 7 cut(s) 113, 170, 203, 407, 470, 532, 751
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 5 cut(s) 216, 938, 999, 1233, 1258
AjnI CCWGG 1 cut(s) 322
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 3 cut(s) 479, 895, 1197
AlwI GGATC 3 cut(s) 232, 568, 754
AlwNI CAGNNNCTG 1 cut(s) 851
ApaI GGGCCC 1 cut(s) 526
ApeKI GCWGC 3 cut(s) 239, 269, 1004
ApoI RAATTY 1 cut(s) 349
Asp700I GAANNNNTTC 1 cut(s) 647
AsuC2I CCSGG 1 cut(s) 1093
AsuHPI GGTGA 1 cut(s) 1144
AsuNHI GCTAGC 2 cut(s) 836, 1394
BaeGI GKGCMC 1 cut(s) 526
BanII GRGCYC 3 cut(s) 95, 526, 1041
BbsI GAAGAC 1 cut(s) 817
Bbv12I GWGCWC 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 3 cut(s) 226, 256, 991
BccI CCATC 4 cut(s) 25, 164, 685, 984
BceAI ACGGC 1 cut(s) 1041
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 1 cut(s) 547
BcnI CCSGG 1 cut(s) 1093
BcoDI GTCTC 3 cut(s) 479, 895, 1197
BfaI CTAG 6 cut(s) 143, 183, 719, 837, 948, 1395
BfmI CTRYAG 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 383
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 4 cut(s) 240, 270, 430, 1005
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 4 cut(s) 241, 271, 431, 1006
Bme1390I CCNGG 2 cut(s) 324, 1093
BmrFI CCNGG 2 cut(s) 324, 1093
BmsI GCATC 4 cut(s) 1239, 1355, 1417, 1439
BmtI GCTAGC 2 cut(s) 840, 1398
BpiI GAAGAC 1 cut(s) 817
BpmI CTGGAG 3 cut(s) 333, 1143, 1179
Bpu10I CCTNAGC 3 cut(s) 857, 1040, 1347
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 944, 1421
BpuMI CCSGG 1 cut(s) 1093
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 16
BsaI GGTCTC 2 cut(s) 479, 895
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 4 cut(s) 193, 223, 1116, 1146
Bsc4I CCNNNNNNNGG 7 cut(s) 113, 170, 203, 407, 470, 532, 751
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 7 cut(s) 118, 208, 226, 430, 1120, 1369, 1451
Bse3DI GCAATG 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 16
BseBI CCWGG 1 cut(s) 324
BseGI GGATG 3 cut(s) 175, 995, 1370
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 7 cut(s) 113, 170, 203, 407, 470, 532, 751
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 7 cut(s) 43, 108, 843, 848, 906, 1054, 1338
BseNI ACTGG 7 cut(s) 118, 208, 226, 430, 1120, 1369, 1451
BseRI GAGGAG 1 cut(s) 734
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 3 cut(s) 226, 256, 991
BseYI CCCAGC 1 cut(s) 328
BsgI GTGCAG 2 cut(s) 744, 792
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 55, 540, 1092
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 7 cut(s) 113, 170, 203, 407, 470, 532, 751
BsmAI GTCTC 3 cut(s) 479, 895, 1197
BsmFI GGGAC 1 cut(s) 285
Bso31I GGTCTC 2 cut(s) 479, 895
Bsp120I GGGCCC 1 cut(s) 522
Bsp1286I GDGCHC 3 cut(s) 95, 526, 1041
Bsp143I GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
Bsp1720I GCTNAGC 1 cut(s) 29
BspACI CCGC 3 cut(s) 359, 430, 1252
BspCNI CTCAG 7 cut(s) 42, 107, 844, 849, 905, 1053, 1339
BspMAI CTGCAG 1 cut(s) 274
BspOI GCTAGC 2 cut(s) 840, 1398
BspPI GGATC 3 cut(s) 232, 568, 754
BspQI GCTCTTC 1 cut(s) 638
BspTI CTTAAG 1 cut(s) 383
BspTNI GGTCTC 2 cut(s) 479, 895
BsrDI GCAATG 1 cut(s) 40
BsrFI RCCGGY 1 cut(s) 54
BsrI ACTGG 7 cut(s) 118, 208, 226, 430, 1120, 1369, 1451
BssAI RCCGGY 1 cut(s) 54
BssMI GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
BssNAI GTATAC 1 cut(s) 960
Bst1107I GTATAC 1 cut(s) 960
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 1288
Bst6I CTCTTC 3 cut(s) 453, 638, 1179
BstAFI CTTAAG 1 cut(s) 383
BstAPI GCANNNNNTGC 1 cut(s) 278
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 5 cut(s) 56, 283, 320, 838, 1396
BstDEI CTNAG 9 cut(s) 29, 94, 177, 614, 852, 857, 892, 1040, 1347
BstENI CCTNNNNNAGG 1 cut(s) 468
BstF5I GGATG 3 cut(s) 175, 995, 1370
BstKTI GATC 6 cut(s) 227, 563, 762, 1243, 1335, 1356
BstMAI GTCTC 3 cut(s) 479, 895, 1197
BstMBI GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
BstMWI GCNNNNNNNGC 4 cut(s) 278, 324, 367, 521
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 322, 1091
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 526
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 226, 256, 991
BstV2I GAAGAC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 960
BsuI GTATCC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 175, 995, 1370
BtsIMutI CAGTG 1 cut(s) 1376
Cac8I GCNNGC 5 cut(s) 56, 283, 320, 838, 1396
CaiI CAGNNNCTG 1 cut(s) 851
Cfr10I RCCGGY 1 cut(s) 54
Csp6I GTAC 3 cut(s) 572, 976, 1267
CviAII CATG 5 cut(s) 103, 319, 371, 792, 1246
CviQI GTAC 3 cut(s) 572, 976, 1267
DdeI CTNAG 9 cut(s) 29, 94, 177, 614, 852, 857, 892, 1040, 1347
DpnI GATC 6 cut(s) 226, 562, 761, 1242, 1334, 1355
DpnII GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
EaeI YGGCCR 2 cut(s) 56, 427
Eam1104I CTCTTC 3 cut(s) 453, 638, 1179
EarI CTCTTC 3 cut(s) 453, 638, 1179
Ecl136II GAGCTC 1 cut(s) 93
Eco105I TACGTA 1 cut(s) 46
Eco24I GRGCYC 3 cut(s) 95, 526, 1041
Eco31I GGTCTC 2 cut(s) 479, 895
Eco32I GATATC 2 cut(s) 124, 160
Eco53kI GAGCTC 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 93
EcoNI CCTNNNNNAGG 1 cut(s) 468
EcoO109I RGGNCCY 3 cut(s) 299, 522, 523
EcoRII CCWGG 1 cut(s) 322
EcoRV GATATC 2 cut(s) 124, 160
EcoT22I ATGCAT 2 cut(s) 739, 1432
EcoT38I GRGCYC 3 cut(s) 95, 526, 1041
FaeI CATG 5 cut(s) 106, 322, 374, 795, 1249
FaqI GGGAC 1 cut(s) 285
FatI CATG 5 cut(s) 102, 318, 370, 791, 1245
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 4 cut(s) 240, 270, 430, 1005
FokI GGATG 3 cut(s) 182, 1002, 1377
FriOI GRGCYC 3 cut(s) 95, 526, 1041
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 4 cut(s) 240, 270, 430, 1005
FspBI CTAG 6 cut(s) 143, 183, 719, 837, 948, 1395
GluI GCNGC 4 cut(s) 240, 270, 430, 1005
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 3 cut(s) 333, 1143, 1179
HapII CCGG 3 cut(s) 55, 540, 1092
Hin1II CATG 5 cut(s) 106, 322, 374, 795, 1249
HinfI GANTC 9 cut(s) 12, 79, 551, 676, 848, 926, 995, 1135, 1151
HpaII CCGG 3 cut(s) 55, 540, 1092
HphI GGTGA 1 cut(s) 1144
Hpy166II GTNNAC 4 cut(s) 592, 730, 960, 1171
Hpy188I TCNGA 3 cut(s) 847, 853, 1081
Hpy8I GTNNAC 4 cut(s) 592, 730, 960, 1171
Hpy99I CGWCG 1 cut(s) 1026
HpyAV CCTTC 5 cut(s) 222, 880, 1046, 1256, 1271
HpyCH4III ACNGT 1 cut(s) 1288
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 6 cut(s) 272, 467, 725, 737, 773, 1430
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 324, 367, 521
HpyF3I CTNAG 9 cut(s) 29, 94, 177, 614, 852, 857, 892, 1040, 1347
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 5 cut(s) 106, 322, 374, 795, 1249
KroI GCCGGC 1 cut(s) 54
KroNI GCCGGC 1 cut(s) 56
Kzo9I GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
LguI GCTCTTC 1 cut(s) 638
LmnI GCTCC 1 cut(s) 1124
Lsp1109I GCAGC 3 cut(s) 226, 256, 991
LweI GCATC 4 cut(s) 1239, 1355, 1417, 1439
MaeI CTAG 6 cut(s) 143, 183, 719, 837, 948, 1395
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 3 cut(s) 415, 901, 1132
MalI GATC 6 cut(s) 226, 562, 761, 1242, 1334, 1355
MboI GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
MboII GAAGA 6 cut(s) 440, 489, 655, 822, 880, 1196
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 3 cut(s) 95, 526, 1041
MluCI AATT 2 cut(s) 349, 878
MlyI GAGTC 4 cut(s) 73, 560, 935, 1129
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 2 cut(s) 739, 1432
MroNI GCCGGC 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 5 cut(s) 338, 384, 1097, 1110, 1272
MslI CAYNNNNRTG 1 cut(s) 1166
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 3 cut(s) 55, 540, 1092
MspR9I CCNGG 2 cut(s) 324, 1093
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 4 cut(s) 278, 324, 367, 521
NaeI GCCGGC 1 cut(s) 56
NciI CCSGG 1 cut(s) 1093
NdeII GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
NgoMIV GCCGGC 1 cut(s) 54
NheI GCTAGC 2 cut(s) 836, 1394
NlaIII CATG 5 cut(s) 106, 322, 374, 795, 1249
NmuCI GTSAC 2 cut(s) 901, 1132
NsiI ATGCAT 2 cut(s) 739, 1432
NspI RCATGY 1 cut(s) 322
PaeI GCATGC 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 638
PdiI GCCGGC 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 5 cut(s) 12, 676, 848, 995, 1151
PkrI GCNGC 4 cut(s) 241, 271, 431, 1006
PleI GAGTC 4 cut(s) 73, 559, 934, 1129
PpsI GAGTC 4 cut(s) 73, 559, 934, 1129
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 322
PspOMI GGGCCC 1 cut(s) 522
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 1 cut(s) 224
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 3 cut(s) 573, 977, 1268
RsaNI GTAC 3 cut(s) 572, 976, 1267
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 5 cut(s) 338, 384, 1097, 1110, 1272
SatI GCNGC 4 cut(s) 240, 270, 430, 1005
Sau3AI GATC 6 cut(s) 224, 560, 759, 1240, 1332, 1353
SchI GAGTC 4 cut(s) 73, 560, 935, 1129
ScrFI CCNGG 2 cut(s) 324, 1093
SduI GDGCHC 3 cut(s) 95, 526, 1041
SfaNI GCATC 4 cut(s) 1239, 1355, 1417, 1439
SfcI CTRYAG 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 3 cut(s) 383, 923, 1400
SmoI CTYRAG 3 cut(s) 383, 923, 1400
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 322
Sse9I AATT 2 cut(s) 349, 878
SsiI CCGC 3 cut(s) 359, 430, 1252
SspI AATATT 1 cut(s) 663
SspMI CTAG 6 cut(s) 143, 183, 719, 837, 948, 1395
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 322, 1091
TaaI ACNGT 1 cut(s) 1288
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 985, 1154
TasI AATT 2 cut(s) 349, 878
TatI WGTACW 1 cut(s) 571
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 5 cut(s) 12, 676, 848, 995, 1151
Tru1I TTAA 5 cut(s) 338, 384, 1097, 1110, 1272
Tru9I TTAA 5 cut(s) 338, 384, 1097, 1110, 1272
TscAI CASTG 1 cut(s) 1376
TseFI GTSAC 2 cut(s) 901, 1132
TseI GCWGC 3 cut(s) 239, 269, 1004
Tsp45I GTSAC 2 cut(s) 901, 1132
TspDTI ATGAA 4 cut(s) 581, 624, 688, 1197
TspRI CASTG 1 cut(s) 1376
Vha464I CTTAAG 1 cut(s) 383
XagI CCTNNNNNAGG 1 cut(s) 468
XapI RAATTY 1 cut(s) 349
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 959
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 6 cut(s) 143, 183, 719, 837, 948, 1395
Zsp2I ATGCAT 2 cut(s) 739, 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.