Rh2BG486000

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001653
Physical Location & Seq
Forward (+)
68612668 .. 68615664
2997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG486000.1

Sequence Viewer

Length: 1449 bp
ATGGGTTCCAGATTCCCATCTCACCAACTCAGCAATGGCCTTTACGTATCAGGCCGGCCAGAGCAACCAAAGGAAAGGACTCCAACTATGAGCTCAGTTGCCACACCTTATACTGGTGGAGATATCAAGAAGTCTGGAGAACTAGGAAAGATGTTTGATATCCCTACAGATGGCTCCAAGTCCAGAAAATCTGGACCTATCACTGGTGGTTCTTCTAGGACTGGATCATTTGGAGGTGCTGCTTCACATTCAGGACCAATTCCTAACGCTGCAGGTCGTGCCGGCTATACTACATCAGGTCCCATATCTGGAGGCATGCCTGGTTCTGCTTCCGTAAAGAAGTCAAATTCTGGACCACTGAATAAGCATGGGGAACCCATGAAGAAGTCATCTGGTCCTCAATCTGGTGGGGTAACACCAACTGGCCGCCAAAACTCCGGGCCTCTTCCCCCTGTACTCCCCACCACAGGTCTCATTACTTCTGGACCCATTTCTTCTGGCCCACTAAATGCTTCTGGAGCCCCTCGAAAGGTATCCGGTCCTTTGGAGTCAATGGGATCAATGAAAGTACAAGGTTCAGTTCACAATCAGGCCATTACTACTCTTAGCCAAGATGATGAGTTTTCGTTTAGGAAAAACTTCCCAAAGCCAATATTATGGTCTTTGATTCTGCTTTTCATGATGGGGTTCATAGCTGGTGGCTTTATTCTCGGTGCGGTCCATAATCCAATTCTCCTCGTCGTGGTTGTGATCCTTTTCAGTGCAGTTGCTGCACTATTCACATGGAATACTTATTGGGGAAGAAGAGCTATTATAGGTTACATTGCTAGTTATTCGGATTCTGAGCTCAGAACTGCCAAGAATGGGCAATTTGTGAAGGTGTCAGGGGTGGTTACTTGTGGTAATGTGCCTCTCGAGTCATCCTTTCAAAAGGTTCCTAGATGTGTGTATACATCCACAAGTTTATATGAGTATCGAGGATGGGATTCAAAAGCTGCGAACCCTACACATCGTCGTTTTTCTTGGGGGCTCAGATCCATGGAAAGACGTGTGGTAGACTTCTACATCTCTGATTTCCAATCTGGGTTGAGAGCATTGGTTAAGACAGGCTGTGGAGCAAGGGTGACACCCTATGTTGATGATTCAATTGTCATTGATGTGAATCCCGTTAATGAAGAGTTGTCTCCAGAGTTTATTAGGTGGTTGAGGGAGAGGAACCTTTCAAGTGATGATCGTGTGATGCAGTTGAAAGAAGGGTACATTAAAGAAGGAAGCACAGTCAGTGTAATGGGGGTCGTCCAGAGAAATGAAAATGTGTTGATGATTGTTCCTCCACCTGAGCCGATCACAACGGGATGCCAATGGGCCAAATGTATCTTCCCAGCTAGCCTTGAGGGCATCGTTTTGAGATGCGATGATGCATCAAAGAATGATGTCATACCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

482

Amino Acids

51.58

Weight (kDa)

9.54

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 950, 1056
AciI CCGC 2 cut(s) 427, 716
AclWI GGATC 4 cut(s) 232, 565, 745, 1029
AcoI YGGCCR 2 cut(s) 56, 424
AcsI RAATTY 1 cut(s) 346
AfaI GTAC 3 cut(s) 456, 570, 1259
AfiI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 742, 864
AflIII ACRYGT 1 cut(s) 1048
AgsI TTSAA 5 cut(s) 929, 990, 1146, 1224, 1249
AjiI CACGTC 1 cut(s) 1049
AjnI CCWGG 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 1361, 1393
AluBI AGCT 6 cut(s) 93, 695, 809, 847, 995, 1385
AluI AGCT 6 cut(s) 93, 695, 809, 847, 995, 1385
Alw21I GWGCWC 2 cut(s) 95, 849
Alw26I GTCTC 2 cut(s) 476, 1188
AlwI GGATC 4 cut(s) 232, 565, 745, 1029
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 914
AoxI GGCC 8 cut(s) 37, 52, 56, 424, 440, 499, 591, 1365
ApeKI GCWGC 4 cut(s) 239, 269, 770, 995
ApoI RAATTY 1 cut(s) 346
Asp700I GAANNNNTTC 1 cut(s) 638
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 14, 1135
AsuNHI GCTAGC 1 cut(s) 1385
AvaI CYCGRG 1 cut(s) 914
AvaII GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 718
BanII GRGCYC 4 cut(s) 95, 523, 849, 1032
Bbv12I GWGCWC 2 cut(s) 95, 849
BbvI GCAGC 4 cut(s) 226, 256, 757, 982
BccI CCATC 4 cut(s) 25, 164, 676, 975
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 544
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 476, 1188
BfaI CTAG 5 cut(s) 143, 216, 828, 939, 1386
BfmI CTRYAG 2 cut(s) 165, 270
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 1395
BisI GCNGC 5 cut(s) 240, 270, 427, 771, 996
BlsI GCNGC 5 cut(s) 241, 271, 428, 772, 997
Bme1390I CCNGG 2 cut(s) 321, 439
Bme18I GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 718
BmeT110I CYCGRG 1 cut(s) 914
BmgBI CACGTC 1 cut(s) 1049
BmiI GGNNCC 8 cut(s) 7, 175, 301, 375, 487, 520, 936, 1217
BmrFI CCNGG 2 cut(s) 321, 439
BmsI GCATC 6 cut(s) 1230, 1346, 1400, 1407, 1408, 1430
BmtI GCTAGC 1 cut(s) 1389
BpmI CTGGAG 4 cut(s) 156, 330, 537, 1170
Bpu10I CCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 1 cut(s) 1412
BpuMI CCSGG 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 2 cut(s) 16, 1161
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 1038
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 2 cut(s) 1107, 1137
Bsc4I CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 742, 864
Bse118I RCCGGY 2 cut(s) 54, 281
Bse1I ACTGG 5 cut(s) 118, 208, 226, 427, 1442
Bse3DI GCAATG 2 cut(s) 40, 822
Bse8I GATNNNNATC 2 cut(s) 16, 1161
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 1038
BseGI GGATG 4 cut(s) 920, 953, 986, 1361
BseJI GATNNNNATC 2 cut(s) 16, 1161
BseLI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 742, 864
BseMI GCAATG 2 cut(s) 40, 822
BseMII CTCAG 6 cut(s) 43, 108, 834, 862, 1045, 1329
BseNI ACTGG 5 cut(s) 118, 208, 226, 427, 1442
BseRI GAGGAG 1 cut(s) 725
BseXI GCAGC 4 cut(s) 226, 256, 757, 982
BseYI CCCAGC 1 cut(s) 1381
BsgI GTGCAG 2 cut(s) 756, 783
BshFI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 593, 1367
BsiHKAI GWGCWC 2 cut(s) 95, 849
BsiHKCI CYCGRG 1 cut(s) 914
BsiSI CCGG 4 cut(s) 55, 282, 438, 537
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 9 cut(s) 113, 170, 203, 308, 404, 467, 529, 742, 864
BsmAI GTCTC 2 cut(s) 476, 1188
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 593, 1367
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 914
Bsp1286I GDGCHC 4 cut(s) 95, 523, 849, 1032
Bsp143I GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 2 cut(s) 427, 716
BspANI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 593, 1367
BspCNI CTCAG 6 cut(s) 42, 107, 835, 861, 1044, 1330
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 8 cut(s) 7, 175, 301, 375, 487, 520, 936, 1217
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 263
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 4 cut(s) 232, 565, 745, 1029
BspQI GCTCTTC 1 cut(s) 799
BspTNI GGTCTC 1 cut(s) 476
BsrDI GCAATG 2 cut(s) 40, 822
BsrFI RCCGGY 2 cut(s) 54, 281
BsrI ACTGG 5 cut(s) 118, 208, 226, 427, 1442
BssAI RCCGGY 2 cut(s) 54, 281
BssECI CCNNGG 1 cut(s) 1038
BssMI GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
BssNAI GTATAC 1 cut(s) 951
BssT1I CCWWGG 1 cut(s) 1038
Bst1107I GTATAC 1 cut(s) 951
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 1279
Bst6I CTCTTC 3 cut(s) 450, 799, 1170
BstAPI GCANNNNNTGC 2 cut(s) 278, 770
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 56, 283, 317, 1387
BstDEI CTNAG 7 cut(s) 29, 94, 605, 843, 848, 1031, 1338
BstDSI CCRYGG 1 cut(s) 1038
BstF5I GGATG 4 cut(s) 920, 953, 986, 1361
BstKTI GATC 6 cut(s) 227, 560, 753, 1037, 1234, 1347
BstMAI GTCTC 2 cut(s) 476, 1188
BstMBI GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
BstMWI GCNNNNNNNGC 4 cut(s) 278, 518, 770, 1395
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 319, 437
BstSFI CTRYAG 2 cut(s) 165, 270
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 4 cut(s) 226, 256, 757, 982
BstX2I RGATCY 1 cut(s) 1034
BstXI CCANNNNNNTGG 1 cut(s) 657
BstYI RGATCY 1 cut(s) 1034
BstZ17I GTATAC 1 cut(s) 951
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 593, 1367
BtgI CCRYGG 1 cut(s) 1038
BtgZI GCGATG 1 cut(s) 1428
BtrI CACGTC 1 cut(s) 1049
BtsCI GGATG 4 cut(s) 920, 953, 986, 1361
BtsIMutI CAGTG 4 cut(s) 201, 356, 766, 1288
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 4 cut(s) 56, 283, 317, 1387
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 1 cut(s) 678
Cfr10I RCCGGY 2 cut(s) 54, 281
Csp6I GTAC 3 cut(s) 455, 569, 1258
CviAII CATG 6 cut(s) 316, 368, 379, 679, 783, 1039
CviQI GTAC 3 cut(s) 455, 569, 1258
DdeI CTNAG 7 cut(s) 29, 94, 605, 843, 848, 1031, 1338
DpnI GATC 6 cut(s) 226, 559, 752, 1036, 1233, 1346
DpnII GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
EaeI YGGCCR 2 cut(s) 56, 424
Eam1104I CTCTTC 3 cut(s) 450, 799, 1170
EarI CTCTTC 3 cut(s) 450, 799, 1170
Ecl136II GAGCTC 2 cut(s) 93, 847
Eco105I TACGTA 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 1038
Eco24I GRGCYC 4 cut(s) 95, 523, 849, 1032
Eco31I GGTCTC 1 cut(s) 476
Eco32I GATATC 2 cut(s) 124, 160
Eco47I GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 718
Eco53kI GAGCTC 2 cut(s) 93, 847
Eco88I CYCGRG 1 cut(s) 914
EcoICRI GAGCTC 2 cut(s) 93, 847
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 319
EcoRV GATATC 2 cut(s) 124, 160
EcoT14I CCWWGG 1 cut(s) 1038
EcoT22I ATGCAT 1 cut(s) 1423
EcoT38I GRGCYC 4 cut(s) 95, 523, 849, 1032
ErhI CCWWGG 1 cut(s) 1038
FaeI CATG 6 cut(s) 319, 371, 382, 682, 786, 1042
FaqI GGGAC 1 cut(s) 285
FatI CATG 6 cut(s) 315, 367, 378, 678, 782, 1038
FblI GTMKAC 2 cut(s) 950, 1056
Fnu4HI GCNGC 5 cut(s) 240, 270, 427, 771, 996
FokI GGATG 4 cut(s) 907, 940, 993, 1368
FriOI GRGCYC 4 cut(s) 95, 523, 849, 1032
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 5 cut(s) 240, 270, 427, 771, 996
FspBI CTAG 5 cut(s) 143, 216, 828, 939, 1386
GluI GCNGC 5 cut(s) 240, 270, 427, 771, 996
GsaI CCCAGC 1 cut(s) 1385
GsuI CTGGAG 4 cut(s) 156, 330, 537, 1170
HaeIII GGCC 8 cut(s) 39, 54, 58, 426, 442, 501, 593, 1367
HapII CCGG 4 cut(s) 55, 282, 438, 537
Hin1II CATG 6 cut(s) 319, 371, 382, 682, 786, 1042
HinfI GANTC 9 cut(s) 12, 79, 548, 667, 839, 917, 986, 1142, 1162
HpaII CCGG 4 cut(s) 55, 282, 438, 537
HphI GGTGA 2 cut(s) 14, 1135
Hpy166II GTNNAC 3 cut(s) 583, 951, 1057
Hpy188I TCNGA 5 cut(s) 838, 844, 851, 1034, 1072
Hpy8I GTNNAC 3 cut(s) 583, 951, 1057
Hpy99I CGWCG 2 cut(s) 743, 1017
HpyAV CCTTC 3 cut(s) 871, 1247, 1262
HpyCH4III ACNGT 1 cut(s) 1279
HpyCH4IV ACGT 2 cut(s) 45, 1048
HpyCH4V TGCA 5 cut(s) 272, 764, 773, 1243, 1421
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 518, 770, 1395
HpyF3I CTNAG 7 cut(s) 29, 94, 605, 843, 848, 1031, 1338
HpySE526I ACGT 2 cut(s) 45, 1048
Hsp92II CATG 6 cut(s) 319, 371, 382, 682, 786, 1042
KroI GCCGGC 2 cut(s) 54, 281
KroNI GCCGGC 2 cut(s) 56, 283
Kzo9I GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
LguI GCTCTTC 1 cut(s) 799
LmnI GCTCC 3 cut(s) 179, 518, 1115
Lsp1109I GCAGC 4 cut(s) 226, 256, 757, 982
LweI GCATC 6 cut(s) 1230, 1346, 1400, 1407, 1408, 1430
MaeI CTAG 5 cut(s) 143, 216, 828, 939, 1386
MaeII ACGT 2 cut(s) 45, 1048
MaeIII GTNAC 4 cut(s) 412, 818, 892, 1123
MalI GATC 6 cut(s) 226, 559, 752, 1036, 1233, 1346
MboI GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
MboII GAAGA 8 cut(s) 204, 394, 437, 486, 813, 816, 1187, 1369
MfeI CAATTG 1 cut(s) 1146
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 4 cut(s) 95, 523, 849, 1032
MluCI AATT 5 cut(s) 258, 346, 729, 869, 1146
MlyI GAGTC 3 cut(s) 73, 557, 926
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 1423
MroNI GCCGGC 2 cut(s) 54, 281
MroXI GAANNNNTTC 1 cut(s) 638
MseI TTAA 3 cut(s) 1101, 1170, 1263
MslI CAYNNNNRTG 1 cut(s) 1157
MspI CCGG 4 cut(s) 55, 282, 438, 537
MspR9I CCNGG 2 cut(s) 321, 439
MunI CAATTG 1 cut(s) 1146
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 4 cut(s) 278, 518, 770, 1395
NaeI GCCGGC 2 cut(s) 56, 283
NciI CCSGG 1 cut(s) 439
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
NgoMIV GCCGGC 2 cut(s) 54, 281
NheI GCTAGC 1 cut(s) 1385
NlaIII CATG 6 cut(s) 319, 371, 382, 682, 786, 1042
NlaIV GGNNCC 8 cut(s) 7, 175, 301, 375, 487, 520, 936, 1217
NmuCI GTSAC 1 cut(s) 1123
NsiI ATGCAT 1 cut(s) 1423
NspI RCATGY 1 cut(s) 319
PaeI GCATGC 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 914
PagI TCATGA 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 799
PdiI GCCGGC 2 cut(s) 56, 283
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 6 cut(s) 12, 667, 839, 986, 1142, 1162
PkrI GCNGC 5 cut(s) 241, 271, 428, 772, 997
PleI GAGTC 3 cut(s) 73, 556, 925
PpsI GAGTC 3 cut(s) 73, 556, 925
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 2 cut(s) 95, 849
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 1381
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 8 cut(s) 7, 175, 301, 375, 487, 520, 936, 1217
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 1 cut(s) 1034
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 3 cut(s) 456, 570, 1259
RsaNI GTAC 3 cut(s) 455, 569, 1258
RseI CAYNNNNRTG 1 cut(s) 1157
SacI GAGCTC 2 cut(s) 95, 849
SapI GCTCTTC 1 cut(s) 799
SaqAI TTAA 3 cut(s) 1101, 1170, 1263
SatI GCNGC 5 cut(s) 240, 270, 427, 771, 996
Sau3AI GATC 6 cut(s) 224, 557, 750, 1034, 1231, 1344
SchI GAGTC 3 cut(s) 73, 557, 926
ScrFI CCNGG 2 cut(s) 321, 439
SduI GDGCHC 4 cut(s) 95, 523, 849, 1032
SfaNI GCATC 6 cut(s) 1230, 1346, 1400, 1407, 1408, 1430
SfcI CTRYAG 2 cut(s) 165, 270
Sfr274I CTCGAG 1 cut(s) 914
SinI GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 718
SlaI CTCGAG 1 cut(s) 914
SmiMI CAYNNNNRTG 1 cut(s) 1157
SmlI CTYRAG 2 cut(s) 914, 1391
SmoI CTYRAG 2 cut(s) 914, 1391
SnaBI TACGTA 1 cut(s) 46
SphI GCATGC 1 cut(s) 319
Sse9I AATT 5 cut(s) 258, 346, 729, 869, 1146
SsiI CCGC 2 cut(s) 427, 716
SspI AATATT 1 cut(s) 654
SspMI CTAG 5 cut(s) 143, 216, 828, 939, 1386
SstI GAGCTC 2 cut(s) 95, 849
StyD4I CCNGG 2 cut(s) 319, 437
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 1 cut(s) 1279
TaiI ACGT 2 cut(s) 48, 1051
TaqI TCGA 3 cut(s) 526, 915, 976
TasI AATT 5 cut(s) 258, 346, 729, 869, 1146
TatI WGTACW 2 cut(s) 454, 568
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 6 cut(s) 12, 667, 839, 986, 1142, 1162
Tru1I TTAA 3 cut(s) 1101, 1170, 1263
Tru9I TTAA 3 cut(s) 1101, 1170, 1263
TscAI CASTG 4 cut(s) 208, 363, 766, 1288
TseFI GTSAC 1 cut(s) 1123
TseI GCWGC 4 cut(s) 239, 269, 770, 995
Tsp45I GTSAC 1 cut(s) 1123
TspDTI ATGAA 6 cut(s) 395, 578, 667, 679, 1188, 1323
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 4 cut(s) 208, 363, 766, 1288
VpaK11BI GGWCC 8 cut(s) 194, 254, 299, 353, 395, 485, 539, 718
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XhoI CTCGAG 1 cut(s) 914
XmiI GTMKAC 2 cut(s) 950, 1056
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 5 cut(s) 143, 216, 828, 939, 1386
Zsp2I ATGCAT 1 cut(s) 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.