RLG00000020436

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013494
Physical Location & Seq
Reverse (-)
64949191 .. 64952470
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020436

Sequence Viewer

Length: 1377 bp
ATGGCAGAAACCAGTACCAGCGGCAGTTGCAGTTGCTCTCCATTGACCCAAATAGCCCAAGACCACCTCTTCACAATCTTGCTACTCCTCCCTGTTGATTCTATTCTCAGCTTTGCCATGACTTGCAAGAAGTTTAGAGCTCTCACAGCTTCTGATACCCTTTGGGAGCACATATGCCAGAGGGACTGGGGCACCAAATCAGTTGAGGCCATCAGGGCTTCAAATTCTAATCAGCAGTTGCCTTGGATGAGGATTTACAAGCAGGTTTCTGGGTTTGACTCTGTGTATTGCCATAGATTGTGTGCTGATCCAGATGCTGAATTGGTGTTTCCAACTCCCAGGGCCTCTCATTCCCTCAATTTTGTATCTGATTGCTTGGTTTTGTTTGGTGGTGGCTGTGAGGGAGGACGCCATCTAGATGACACATGGGTGGCATACATGGGTAATGATTTTCAGAGACTGCTGAAGTGGCAGAGGATCAGTTCGGGCAGTCCAAGTGGAAGATTTGGGCATACATGTGCTGTAATTGATGATTTTCTTGTTCTATTTGGAGGTATAAATGACAATGGAAACCGTCAAAACGATACATGGGTGGGGCAAGTAGCATGCCATGATAGCCTTGGTATCACATTGTCCTGGAGGCTGCTTGTGGTGGGGGACAATGCACCTCCCCAGCGAGGAGCTCATGCGGCATGCTGCATTGAAGGTAGAAAGATGGTTATCCATGGAGGAATTGGGCGGTTTGGTGTGAGACTGAGTGACACATGGGTATTGGAACTTTCAGAAAACTTTCGAACTGGTACTTGGTATGAAATCGTTACTCAGCCATCTCCCCCAGCTCGCTCAGGTCACACGTTGACTTGCATTGGGGGAACCCGAACTGTTTTATTTGGAGGAAGAGGATTGGGATATGATGTGCTTCATGATCTTTGGCTCTTGGATATTTGTGAGGGTCATCCACAATGGTTACAAGTACTATACGAGTTACAAAATATTCCTTGTGGAGTTTCCCTTCCACGGGTTGGTCACTCGGCTACACTCATCTTAGGAGGTCGTTTGCTGATATGTGGAGGGGAAGACTCGGATAGACACCGGAAGAATGACTTCTGGGTTTTAGACATTACTGCAGTTCCCTCCATTACGGTGCAGCCAACTATGTTGAACTCTATGCATTTATTTGCAAAAATGTGGAAAAGATTGAAGACAAAGGATCATATACCCAACTGCCGGTCATTTCATCGTGCCTGTGCAGATCAATCTGGGCGTTGCGTGTTTATGTTTGGCGGAATGGTGGATGGTATACTTCAACCTGCTGACCCAGCTGGGCTGAGGTTTGATGGAGAGCTCCTCCTTGTGGAGCTCGTGCTTCAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_3 Kelch_4 Kelch_5

Protein Analysis

459

Amino Acids

50.67

Weight (kDa)

6.48

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 23 - 61 2e-06 F-box-like
Beta-prop_FBX42 PF13415 107 - 263 2.2e-23 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 108 - 262 8.2e-24 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 156 - 245 6.5e-08 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 156 - 431 1.6e-10 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 210 - 375 2.9e-25 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 263 - 375 3.6e-14 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 263 - 431 2.8e-07 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 278 - 430 8.4e-09 tRNA wybutosine-synthesizing protein 4, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51550
fragaria_vesca FvH4_6g37110
malus_domestica MD09G1161500.v1.1 MD17G1147400.v1.1
prunus_persica Prupe.3G020600_v2.0.a1
pyrus_communis pycom09g08000 pycom09g08050 pycom17g14000
rosa_chinensis RchiOBHm_Chr2g0149671
rosa_laevigata RLG00000020436
rosa_multiflora Rmu_sc0014307.1_g000005
rosa_roxburghii Rroxscaffold_2G00097810
rosa_rugosa Rorug02G0414300
rosa_samantha Rh2AG474500 Rh2BG486200 Rh2CG460200 Rh2DG495500
rosa_wichuraiana Rw2G038690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 253, 1318
AccB1I GGYRCC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccI GTMKAC 1 cut(s) 1300
AciI CCGC 4 cut(s) 21, 689, 739, 1284
AclWI GGATC 3 cut(s) 302, 485, 1218
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 2 cut(s) 485, 1352
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 3 cut(s) 16, 802, 975
AfiI CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
AflIII ACRYGT 2 cut(s) 515, 852
AgsI TTSAA 5 cut(s) 222, 704, 1162, 1201, 1307
AjnI CCWGG 2 cut(s) 338, 635
AjuI GAANNNNNNNTTGG 2 cut(s) 787, 819
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 8 cut(s) 111, 140, 149, 683, 839, 1322, 1345, 1360
AluI AGCT 8 cut(s) 111, 140, 149, 683, 839, 1322, 1345, 1360
Alw21I GWGCWC 5 cut(s) 142, 171, 685, 1347, 1362
Alw26I GTCTC 2 cut(s) 451, 745
AlwI GGATC 3 cut(s) 302, 485, 1218
AlwNI CAGNNNCTG 3 cut(s) 152, 317, 460
AoxI GGCC 2 cut(s) 207, 342
ApeKI GCWGC 3 cut(s) 643, 696, 1147
ApoI RAATTY 1 cut(s) 223
Asp700I GAANNNNTTC 2 cut(s) 789, 1103
AspS9I GGNCC 1 cut(s) 342
AsuII TTCGAA 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BanI GGYRCC 1 cut(s) 191
BanII GRGCYC 4 cut(s) 142, 685, 1347, 1362
BauI CACGAG 1 cut(s) 1361
BbsI GAAGAC 2 cut(s) 1083, 1208
Bbv12I GWGCWC 5 cut(s) 142, 171, 685, 1347, 1362
BbvCI CCTCAGC 1 cut(s) 1328
BbvI GCAGC 3 cut(s) 630, 683, 1159
BccI CCATC 6 cut(s) 218, 420, 709, 835, 1289, 1331
BciT130I CCWGG 2 cut(s) 340, 637
BcoDI GTCTC 2 cut(s) 451, 745
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 1125
BfuAI ACCTGC 2 cut(s) 253, 1318
BglI GCCNNNNNGGC 1 cut(s) 215
BisI GCNGC 5 cut(s) 22, 644, 690, 697, 1148
BlsI GCNGC 5 cut(s) 23, 645, 691, 698, 1149
BmcAI AGTACT 1 cut(s) 975
Bme1390I CCNGG 2 cut(s) 340, 637
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 2 cut(s) 193, 874
BmrFI CCNGG 2 cut(s) 340, 637
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 2 cut(s) 1083, 1208
BplI GAGNNNNNCTC 2 cut(s) 1332, 1364
BpmI CTGGAG 1 cut(s) 658
Bpu10I CCTNAGC 2 cut(s) 844, 1328
Bpu14I TTCGAA 1 cut(s) 793
BsaBI GATNNNNATC 1 cut(s) 719
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 6 cut(s) 242, 338, 339, 619, 724, 1016
BsaWI WCCGGW 1 cut(s) 1092
Bsc4I CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
Bse118I RCCGGY 1 cut(s) 1227
Bse1I ACTGG 3 cut(s) 12, 191, 802
Bse8I GATNNNNATC 1 cut(s) 719
BseBI CCWGG 2 cut(s) 340, 637
BseDI CCNNGG 6 cut(s) 242, 338, 339, 619, 724, 1016
BseGI GGATG 3 cut(s) 252, 955, 1300
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
BseMII CTCAG 5 cut(s) 121, 746, 836, 858, 1319
BseNI ACTGG 3 cut(s) 12, 191, 802
BseRI GAGGAG 3 cut(s) 77, 693, 1337
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 3 cut(s) 630, 683, 1159
BseYI CCCAGC 4 cut(s) 672, 835, 1318, 1322
BsgI GTGCAG 2 cut(s) 1166, 1269
BshFI GGCC 2 cut(s) 209, 344
BshNI GGYRCC 1 cut(s) 191
BsiHKAI GWGCWC 5 cut(s) 142, 171, 685, 1347, 1362
BsiSI CCGG 2 cut(s) 1093, 1228
BslFI GGGAC 2 cut(s) 197, 671
BslI CCNNNNNNNGG 6 cut(s) 677, 1017, 1018, 1022, 1227, 1354
BsmAI GTCTC 2 cut(s) 451, 745
BsmFI GGGAC 2 cut(s) 197, 671
BsnI GGCC 2 cut(s) 209, 344
Bsp119I TTCGAA 1 cut(s) 793
Bsp1286I GDGCHC 6 cut(s) 142, 171, 194, 685, 1347, 1362
Bsp143I GATC 5 cut(s) 307, 477, 925, 1210, 1252
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 4 cut(s) 21, 689, 739, 1284
BspANI GGCC 2 cut(s) 209, 344
BspCNI CTCAG 5 cut(s) 120, 747, 835, 857, 1320
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 2 cut(s) 193, 874
BspMAI CTGCAG 1 cut(s) 1129
BspMI ACCTGC 2 cut(s) 253, 1318
BspPI GGATC 3 cut(s) 302, 485, 1218
BspT104I TTCGAA 1 cut(s) 793
BspT107I GGYRCC 1 cut(s) 191
BsrFI RCCGGY 1 cut(s) 1227
BsrI ACTGG 3 cut(s) 12, 191, 802
BssAI RCCGGY 1 cut(s) 1227
BssECI CCNNGG 6 cut(s) 242, 338, 339, 619, 724, 1016
BssMI GATC 5 cut(s) 307, 477, 925, 1210, 1252
BssNAI GTATAC 1 cut(s) 1301
BssNI GRCGYC 1 cut(s) 409
BssSI CACGAG 1 cut(s) 1361
BssT1I CCWWGG 3 cut(s) 242, 619, 724
Bst1107I GTATAC 1 cut(s) 1301
Bst2BI CACGAG 1 cut(s) 1361
Bst2UI CCWGG 2 cut(s) 340, 637
Bst4CI ACNGT 3 cut(s) 575, 883, 1144
Bst6I CTCTTC 2 cut(s) 74, 892
BstACI GRCGYC 1 cut(s) 409
BstBI TTCGAA 1 cut(s) 793
BstC8I GCNNGC 3 cut(s) 607, 694, 841
BstDEI CTNAG 6 cut(s) 107, 755, 822, 844, 1045, 1328
BstDSI CCRYGG 2 cut(s) 724, 1016
BstF5I GGATG 3 cut(s) 252, 955, 1300
BstKTI GATC 5 cut(s) 310, 480, 928, 1213, 1255
BstMAI GTCTC 2 cut(s) 451, 745
BstMBI GATC 5 cut(s) 307, 477, 925, 1210, 1252
BstMWI GCNNNNNNNGC 7 cut(s) 27, 146, 215, 469, 615, 689, 1319
BstNI CCWGG 2 cut(s) 340, 637
BstNSI RCATGY 3 cut(s) 519, 609, 696
BstSCI CCNGG 2 cut(s) 338, 635
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 3 cut(s) 630, 683, 1159
BstV2I GAAGAC 2 cut(s) 1083, 1208
BstZ17I GTATAC 1 cut(s) 1301
BsuRI GGCC 2 cut(s) 209, 344
BtgI CCRYGG 2 cut(s) 724, 1016
BtsCI GGATG 3 cut(s) 252, 955, 1300
BveI ACCTGC 2 cut(s) 253, 1318
Cac8I GCNNGC 3 cut(s) 607, 694, 841
CaiI CAGNNNCTG 3 cut(s) 152, 317, 460
CciI TCATGA 1 cut(s) 922
Cfr10I RCCGGY 1 cut(s) 1227
Cfr13I GGNCC 1 cut(s) 342
CseI GACGC 1 cut(s) 417
Csp6I GTAC 3 cut(s) 15, 801, 974
CviQI GTAC 3 cut(s) 15, 801, 974
DdeI CTNAG 6 cut(s) 107, 755, 822, 844, 1045, 1328
DpnI GATC 5 cut(s) 309, 479, 927, 1212, 1254
DpnII GATC 5 cut(s) 307, 477, 925, 1210, 1252
Eam1104I CTCTTC 2 cut(s) 74, 892
EarI CTCTTC 2 cut(s) 74, 892
EciI GGCGGA 1 cut(s) 1299
Ecl136II GAGCTC 4 cut(s) 140, 683, 1345, 1360
Eco130I CCWWGG 3 cut(s) 242, 619, 724
Eco24I GRGCYC 4 cut(s) 142, 685, 1347, 1362
Eco53kI GAGCTC 4 cut(s) 140, 683, 1345, 1360
Eco57I CTGAAG 2 cut(s) 485, 1352
EcoICRI GAGCTC 4 cut(s) 140, 683, 1345, 1360
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 338, 635
EcoT14I CCWWGG 3 cut(s) 242, 619, 724
EcoT22I ATGCAT 1 cut(s) 1173
EcoT38I GRGCYC 4 cut(s) 142, 685, 1347, 1362
ErhI CCWWGG 3 cut(s) 242, 619, 724
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 2 cut(s) 197, 671
FauNDI CATATG 1 cut(s) 173
FblI GTMKAC 1 cut(s) 1300
Fnu4HI GCNGC 5 cut(s) 22, 644, 690, 697, 1148
FokI GGATG 3 cut(s) 259, 942, 1307
FriOI GRGCYC 4 cut(s) 142, 685, 1347, 1362
Fsp4HI GCNGC 5 cut(s) 22, 644, 690, 697, 1148
FspBI CTAG 1 cut(s) 416
GluI GCNGC 5 cut(s) 22, 644, 690, 697, 1148
GsaI CCCAGC 4 cut(s) 676, 839, 1322, 1326
GsuI CTGGAG 1 cut(s) 658
HaeIII GGCC 2 cut(s) 209, 344
HapII CCGG 2 cut(s) 1093, 1228
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 409
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 3 cut(s) 98, 278, 1079
HpaII CCGG 2 cut(s) 1093, 1228
Hpy166II GTNNAC 2 cut(s) 858, 1301
Hpy188I TCNGA 5 cut(s) 154, 370, 456, 784, 1084
Hpy188III TCNNGA 3 cut(s) 311, 416, 923
Hpy8I GTNNAC 2 cut(s) 858, 1301
HpyAV CCTTC 2 cut(s) 698, 1022
HpyCH4III ACNGT 3 cut(s) 575, 883, 1144
HpyCH4IV ACGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 146, 215, 469, 615, 689, 1319
HpyF3I CTNAG 6 cut(s) 107, 755, 822, 844, 1045, 1328
HpySE526I ACGT 1 cut(s) 854
Hsp92I GRCGYC 1 cut(s) 409
Kzo9I GATC 5 cut(s) 307, 477, 925, 1210, 1252
LmnI GCTCC 4 cut(s) 166, 680, 1350, 1357
Lsp1109I GCAGC 3 cut(s) 630, 683, 1159
LweI GCATC 1 cut(s) 304
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 6 cut(s) 758, 817, 848, 966, 984, 1025
MalI GATC 5 cut(s) 309, 479, 927, 1212, 1254
MboI GATC 5 cut(s) 307, 477, 925, 1210, 1252
MboII GAAGA 6 cut(s) 61, 513, 909, 1088, 1108, 1213
MhlI GDGCHC 6 cut(s) 142, 171, 194, 685, 1347, 1362
MluCI AATT 5 cut(s) 223, 320, 358, 525, 732
MlyI GAGTC 2 cut(s) 272, 1073
MmeI TCCRAC 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 1173
MroXI GAANNNNTTC 2 cut(s) 789, 1103
MslI CAYNNNNRTG 4 cut(s) 417, 428, 516, 1142
MspA1I CMGCKG 2 cut(s) 21, 1322
MspI CCGG 2 cut(s) 1093, 1228
MspR9I CCNGG 2 cut(s) 340, 637
MvaI CCWGG 2 cut(s) 340, 637
MwoI GCNNNNNNNGC 7 cut(s) 27, 146, 215, 469, 615, 689, 1319
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 173
NdeII GATC 5 cut(s) 307, 477, 925, 1210, 1252
NlaIV GGNNCC 2 cut(s) 193, 874
NmeAIII GCCGAG 1 cut(s) 1010
NmuCI GTSAC 3 cut(s) 758, 848, 1025
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 3 cut(s) 519, 609, 696
NspV TTCGAA 1 cut(s) 793
OliI CACNNNNGTG 1 cut(s) 428
PaeI GCATGC 2 cut(s) 609, 696
PagI TCATGA 1 cut(s) 922
PasI CCCWGGG 1 cut(s) 339
PciI ACATGT 1 cut(s) 515
PdmI GAANNNNTTC 2 cut(s) 789, 1103
PfeI GAWTC 1 cut(s) 98
PflMI CCANNNNNTGG 1 cut(s) 1022
PfoI TCCNGGA 1 cut(s) 635
PkrI GCNGC 5 cut(s) 23, 645, 691, 698, 1149
PleI GAGTC 2 cut(s) 272, 1073
PpsI GAGTC 2 cut(s) 272, 1073
PscI ACATGT 1 cut(s) 515
Psp124BI GAGCTC 4 cut(s) 142, 685, 1347, 1362
Psp6I CCWGG 2 cut(s) 338, 635
PspFI CCCAGC 4 cut(s) 672, 835, 1318, 1322
PspGI CCWGG 2 cut(s) 338, 635
PspN4I GGNNCC 2 cut(s) 193, 874
PspPI GGNCC 1 cut(s) 342
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 3 cut(s) 152, 317, 460
PvuII CAGCTG 1 cut(s) 1322
RsaI GTAC 3 cut(s) 16, 802, 975
RsaNI GTAC 3 cut(s) 15, 801, 974
RseI CAYNNNNRTG 4 cut(s) 417, 428, 516, 1142
SacI GAGCTC 4 cut(s) 142, 685, 1347, 1362
SatI GCNGC 5 cut(s) 22, 644, 690, 697, 1148
Sau3AI GATC 5 cut(s) 307, 477, 925, 1210, 1252
Sau96I GGNCC 1 cut(s) 342
ScaI AGTACT 1 cut(s) 975
SchI GAGTC 2 cut(s) 272, 1073
ScrFI CCNGG 2 cut(s) 340, 637
SduI GDGCHC 6 cut(s) 142, 171, 194, 685, 1347, 1362
SfaNI GCATC 1 cut(s) 304
SfcI CTRYAG 1 cut(s) 1125
SfuI TTCGAA 1 cut(s) 793
SmiMI CAYNNNNRTG 4 cut(s) 417, 428, 516, 1142
SphI GCATGC 2 cut(s) 609, 696
Sse9I AATT 5 cut(s) 223, 320, 358, 525, 732
SsiI CCGC 4 cut(s) 21, 689, 739, 1284
SspI AATATT 1 cut(s) 994
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 4 cut(s) 142, 685, 1347, 1362
StyD4I CCNGG 2 cut(s) 338, 635
StyI CCWWGG 3 cut(s) 242, 619, 724
TaaI ACNGT 3 cut(s) 575, 883, 1144
TaiI ACGT 1 cut(s) 857
TaqI TCGA 1 cut(s) 793
TasI AATT 5 cut(s) 223, 320, 358, 525, 732
TatI WGTACW 1 cut(s) 973
TauI GCSGC 2 cut(s) 24, 692
TfiI GAWTC 1 cut(s) 98
TseFI GTSAC 3 cut(s) 758, 848, 1025
TseI GCWGC 3 cut(s) 643, 696, 1147
Tsp45I GTSAC 3 cut(s) 758, 848, 1025
TspDTI ATGAA 3 cut(s) 825, 911, 1226
Van91I CCANNNNNTGG 1 cut(s) 1022
XapI RAATTY 1 cut(s) 223
XbaI TCTAGA 1 cut(s) 415
XceI RCATGY 3 cut(s) 519, 609, 696
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 731
XmiI GTMKAC 1 cut(s) 1300
XmnI GAANNNNTTC 2 cut(s) 789, 1103
XspI CTAG 1 cut(s) 416
ZrmI AGTACT 1 cut(s) 975
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.