Prupe.3G042000_v2.0.a1

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0010758
Physical Location & Seq
Reverse (-)
2991593 .. 2995356
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G042000.1

Sequence Viewer

Length: 1758 bp
ATGAGTAACTGGTTGGGGTTTTCTCTCACTCCACACTTGAGGATCCATGAAGGCTATGGTGGGGGAGAAGATCAACAAGACCATGGAGGAGGAGGAGGAGGAGGAGGAGGCTTCACTTCTCAAATGACCATCATGCCCTTGCGCTCTGATGGGTCTCTTTGTGTTCCTCCTGCTGATCCTTTCAGGCGACCCAATGGTTCTGAAGATTGGAGATATGAAAACCACATGGGTGCAACAAGTGTCACAGACCCAAATGATGAAGGGGGTCCAAAGCTTGAAGATTTCTTGGGCAGCTGCTACTCAAGCTCTCCCCCTCCAAGTGAAACCAAAGTCTACAATTGCCAAAGTACCCAACAAGACCAAAACCCAGAAACCATCTCTCAGATCAATGTTAATGAGGCCCCAAACTTCAACCCCACCACCAACAATGGAGAAGACAACAACAACAACCACCACCTAATTCAACCATACAGCCATTATTGTACTCTAAACCCACCAAGCCTCATCCAAAGTAATGGCATGTACAAATCTTGGTTGGGCCAAACGGTACCGTTTTCTGATGAAAAATCAACACCATGTGGTGATCACCACCAGGCAAATGGCAACAGCTTTCATCAGTCATCTCTCTCTCTCACTATGAGCCCAAGCTTACAACAAAATGGTAATAATATGGGCGCAATCTCTCAACTGCAAATAGTGGAAAGCAAGAAAAGGCCTGTTGGGAAATCTGCAGCTAGAGAACCAGTTCCTAGAAAATCTATTGACACTTTTGGTCAGCGAACTTCTCAGTTCCGTGGTGTCACCAGGCATAGGTGGACTGGGAGATATGAGGCCCATCTGTGGGATAATAGTTGCAGAAAAGAAGGGCAAACAAGGAAAGGAAGGCAAGTTTATCTGGGTGGTTATGATAAGGAGGAAAAAGCAGCAAGGGCATATGATTTAGCTGCACTCAAGTATTGGGGTTCAACTACCCACATTAATTTTCCTCTAAGTACTTATGAGAGAGAGTTGGAAGAGATGAAGAACATGACAAGGCAAGAATTTGTAGCCAATTTGAGAAGGAAAAGCAGTGGGTTTTCAAGAGGAGCTTCTGTGTACAGAGGAGTGACAAGACACCATCAGCATGGAAGGTGGCAAGCTAGAATTGGCAGGGTGGCTGGAAACAAAGACTTGTACTTGGGAACATTTAGCACACAAGAAGAAGCAGCTGAGGCTTATGACATTGCTGCAATCAAGTTTAGGGGAACAAGTGCTGTAACCAACTTTGATATGAGTAGGTATGATGTGAAGAGAATTTGCTCAAGCTCCACCCTAATTGCCAGTGAGCTGGCCAAGCGTTTGCCGAAAGACTCTACTCCGGCGGCAATCGAAGACTTCAATTCTTGTGGATCATCTACTTCTGCACAACCCCTTTTGGCCATAGCAAGTGGTGAACCGTGTGATGACATGGCTGAAATGGTGTGGAACGGTAATAATGCAGAGGATCATTCCCATTCACATCATGAGAATTCAAACACCACCAACAATGCAGCATTGGGTGCTTCAAGCAGCAGGAACTCTTCAAGCCCACAAAGCCCCAAGTGCTCATTTGGCCACACTAGTGAGTTTGGCCTTGGTGGAGGAGACTATTCCCATGCTTATTTCTCATTGCAGGGTGCTAAGTATGAAGATGGCAATGGTGGTGGGAATGATCATTCAAGGCTTGGAAATCTTGGTTTGGTTCCACAAGTGCCTATGTTTGCACTATGGAATGACTGA

Protein Analysis

586

Amino Acids

64.17

Weight (kDa)

6.37

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72570
fragaria_vesca FvH4_6g34710
malus_domestica MD00G1092000.v1.1 MD09G1183700.v1.1
prunus_persica Prupe.3G042000_v2.0.a1
pyrus_communis pycom09g10110
rosa_chinensis RchiOBHm_Chr2g0145271
rosa_laevigata RLG00000020135
rosa_multiflora Rmu_sc0001298.1_g000029
rosa_roxburghii Rroxscaffold_2G00100770
rosa_rugosa Rorug02G0391600
rosa_samantha Rh2AG444400 Rh2AG444500 Rh2BG455400 Rh2BG455500 Rh2CG431400 Rh2CG431500 Rh2DG465400 Rh2DG465500
rosa_wichuraiana Rw2G036320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 547
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 1 cut(s) 333
AciI CCGC 1 cut(s) 1361
AclWI GGATC 5 cut(s) 37, 50, 170, 1396, 1491
AcoI YGGCCR 3 cut(s) 1329, 1416, 1591
AcsI RAATTY 3 cut(s) 1040, 1293, 1507
AcuI CTGAAG 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 578
AfaI GTAC 7 cut(s) 349, 484, 524, 549, 994, 1097, 1175
AfiI CCNNNNNNNGG 3 cut(s) 810, 840, 841
AhlI ACTAGT 1 cut(s) 1598
AjnI CCWGG 2 cut(s) 591, 803
AleI CACNNNNGTG 2 cut(s) 228, 1599
Alw21I GWGCWC 1 cut(s) 1586
Alw26I GTCTC 2 cut(s) 159, 1617
AlwI GGATC 5 cut(s) 37, 50, 170, 1396, 1491
AoxI GGCC 8 cut(s) 399, 538, 713, 831, 1329, 1416, 1591, 1609
ApeKI GCWGC 9 cut(s) 291, 294, 731, 923, 944, 1205, 1226, 1529, 1548
ApoI RAATTY 3 cut(s) 1040, 1293, 1507
AseI ATTAAT 1 cut(s) 978
Asp700I GAANNNNTTC 1 cut(s) 744
Asp718I GGTACC 1 cut(s) 547
AspLEI GCGC 2 cut(s) 144, 677
AspS9I GGNCC 4 cut(s) 266, 400, 538, 832
AsuHPI GGTGA 4 cut(s) 578, 593, 793, 1442
AvaII GGWCC 1 cut(s) 266
BalI TGGCCA 3 cut(s) 1331, 1418, 1593
BamHI GGATCC 1 cut(s) 42
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 644
BbsI GAAGAC 2 cut(s) 441, 1377
Bbv12I GWGCWC 1 cut(s) 1586
BbvCI CCTCAGC 1 cut(s) 1209
BbvI GCAGC 9 cut(s) 281, 303, 743, 931, 935, 1213, 1217, 1541, 1560
BccI CCATC 6 cut(s) 137, 143, 383, 843, 1125, 1664
BciT130I CCWGG 2 cut(s) 593, 805
BclI TGATCA 2 cut(s) 583, 1690
BcoDI GTCTC 2 cut(s) 159, 1617
BcuI ACTAGT 1 cut(s) 1598
BfaI CTAG 4 cut(s) 735, 750, 1140, 1599
BfmI CTRYAG 1 cut(s) 729
BmcAI AGTACT 1 cut(s) 994
Bme1390I CCNGG 2 cut(s) 593, 805
Bme18I GGWCC 1 cut(s) 266
BmgT120I GGNCC 4 cut(s) 266, 400, 538, 832
BmiI GGNNCC 5 cut(s) 44, 267, 402, 549, 1722
BmrFI CCNGG 2 cut(s) 593, 805
BmrI ACTGGG 1 cut(s) 828
BmuI ACTGGG 1 cut(s) 828
BpiI GAAGAC 2 cut(s) 441, 1377
Bpu10I CCTNAGC 1 cut(s) 1209
BpuEI CTTGAG 4 cut(s) 58, 286, 935, 1285
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 82, 793, 1612
BsaXI ACNNNNNCTCC 2 cut(s) 1077, 1107
Bsc4I CCNNNNNNNGG 3 cut(s) 810, 840, 841
Bse1I ACTGG 4 cut(s) 14, 743, 823, 1320
Bse3DI GCAATG 3 cut(s) 1221, 1646, 1681
BseBI CCWGG 2 cut(s) 593, 805
BseDI CCNNGG 3 cut(s) 82, 793, 1612
BseGI GGATG 1 cut(s) 504
BseLI CCNNNNNNNGG 3 cut(s) 810, 840, 841
BseMI GCAATG 3 cut(s) 1221, 1646, 1681
BseMII CTCAG 3 cut(s) 395, 800, 1200
BseNI ACTGG 4 cut(s) 14, 743, 823, 1320
BseXI GCAGC 9 cut(s) 281, 303, 743, 931, 935, 1213, 1217, 1541, 1560
BsgI GTGCAG 2 cut(s) 930, 1386
BshFI GGCC 8 cut(s) 401, 540, 715, 833, 1331, 1418, 1593, 1611
BshNI GGYRCC 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 1586
BsiSI CCGG 1 cut(s) 1358
BslI CCNNNNNNNGG 3 cut(s) 810, 840, 841
BsmAI GTCTC 2 cut(s) 159, 1617
BsnI GGCC 8 cut(s) 401, 540, 715, 833, 1331, 1418, 1593, 1611
Bso31I GGTCTC 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 644, 1586
Bsp1407I TGTACA 2 cut(s) 522, 1095
Bsp143I GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 1 cut(s) 1361
BspANI GGCC 8 cut(s) 401, 540, 715, 833, 1331, 1418, 1593, 1611
BspCNI CTCAG 3 cut(s) 394, 799, 1201
BspHI TCATGA 1 cut(s) 1501
BspLI GGNNCC 5 cut(s) 44, 267, 402, 549, 1722
BspMAI CTGCAG 1 cut(s) 733
BspPI GGATC 5 cut(s) 37, 50, 170, 1396, 1491
BspT107I GGYRCC 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 159
BsrDI GCAATG 3 cut(s) 1221, 1646, 1681
BsrGI TGTACA 2 cut(s) 522, 1095
BsrI ACTGG 4 cut(s) 14, 743, 823, 1320
BssECI CCNNGG 3 cut(s) 82, 793, 1612
BssMI GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
BssT1I CCWWGG 2 cut(s) 82, 1612
Bst2UI CCWGG 2 cut(s) 593, 805
Bst4CI ACNGT 4 cut(s) 547, 552, 1437, 1469
Bst6I CTCTTC 3 cut(s) 1008, 1283, 1564
BstAPI GCANNNNNTGC 1 cut(s) 1538
BstAUI TGTACA 2 cut(s) 522, 1095
BstC8I GCNNGC 2 cut(s) 1137, 1329
BstDEI CTNAG 5 cut(s) 381, 786, 989, 1209, 1659
BstDSI CCRYGG 2 cut(s) 82, 793
BstF5I GGATG 1 cut(s) 504
BstHHI GCGC 2 cut(s) 144, 677
BstKTI GATC 8 cut(s) 45, 73, 178, 387, 586, 1391, 1486, 1693
BstMAI GTCTC 2 cut(s) 159, 1617
BstMBI GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
BstMWI GCNNNNNNNGC 8 cut(s) 303, 929, 1211, 1333, 1538, 1572, 1581, 1590
BstNI CCWGG 2 cut(s) 593, 805
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 2 cut(s) 591, 803
BstSFI CTRYAG 1 cut(s) 729
BstV1I GCAGC 9 cut(s) 281, 303, 743, 931, 935, 1213, 1217, 1541, 1560
BstV2I GAAGAC 2 cut(s) 441, 1377
BstX2I RGATCY 1 cut(s) 42
BstXI CCANNNNNNTGG 4 cut(s) 515, 599, 1124, 1327
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 8 cut(s) 401, 540, 715, 833, 1331, 1418, 1593, 1611
BtgI CCRYGG 2 cut(s) 82, 793
BtsCI GGATG 1 cut(s) 504
BtsI GCAGTG 1 cut(s) 1075
BtsIMutI CAGTG 2 cut(s) 1075, 1327
Cac8I GCNNGC 2 cut(s) 1137, 1329
CciI TCATGA 1 cut(s) 1501
CfoI GCGC 2 cut(s) 144, 677
Cfr13I GGNCC 4 cut(s) 266, 400, 538, 832
Csp6I GTAC 7 cut(s) 348, 483, 523, 548, 993, 1096, 1174
CspCI CAANNNNNGTGG 6 cut(s) 1297, 1332, 1366, 1401, 1663, 1698
CviQI GTAC 7 cut(s) 348, 483, 523, 548, 993, 1096, 1174
DdeI CTNAG 5 cut(s) 381, 786, 989, 1209, 1659
DpnI GATC 8 cut(s) 44, 72, 177, 386, 585, 1390, 1485, 1692
DpnII GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
DraIII CACNNNGTG 1 cut(s) 578
EaeI YGGCCR 3 cut(s) 1329, 1416, 1591
Eam1104I CTCTTC 3 cut(s) 1008, 1283, 1564
EarI CTCTTC 3 cut(s) 1008, 1283, 1564
Eco130I CCWWGG 2 cut(s) 82, 1612
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 644
Eco31I GGTCTC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 1507
EcoRII CCWGG 2 cut(s) 591, 803
EcoT14I CCWWGG 2 cut(s) 82, 1612
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 2 cut(s) 82, 1612
FalI AAGNNNNNCTT 2 cut(s) 1072, 1104
FauNDI CATATG 1 cut(s) 934
FbaI TGATCA 2 cut(s) 583, 1690
FblI GTMKAC 1 cut(s) 333
FokI GGATG 1 cut(s) 491
FriOI GRGCYC 1 cut(s) 644
FspBI CTAG 4 cut(s) 735, 750, 1140, 1599
GlaI GCGC 2 cut(s) 143, 676
HaeIII GGCC 8 cut(s) 401, 540, 715, 833, 1331, 1418, 1593, 1611
HapII CCGG 1 cut(s) 1358
HhaI GCGC 2 cut(s) 144, 677
Hin6I GCGC 2 cut(s) 142, 675
HinP1I GCGC 2 cut(s) 142, 675
HindIII AAGCTT 2 cut(s) 272, 646
HinfI GANTC 1 cut(s) 1349
HpaII CCGG 1 cut(s) 1358
HphI GGTGA 4 cut(s) 578, 593, 793, 1442
Hpy166II GTNNAC 4 cut(s) 334, 816, 1096, 1433
Hpy188I TCNGA 4 cut(s) 148, 202, 384, 559
Hpy188III TCNNGA 2 cut(s) 1080, 1502
Hpy8I GTNNAC 4 cut(s) 334, 816, 1096, 1433
HpyAV CCTTC 6 cut(s) 44, 254, 857, 876, 1053, 1122
HpyCH4III ACNGT 4 cut(s) 547, 552, 1437, 1469
HpyF10VI GCNNNNNNNGC 8 cut(s) 303, 929, 1211, 1333, 1538, 1572, 1581, 1590
HpyF3I CTNAG 5 cut(s) 381, 786, 989, 1209, 1659
HspAI GCGC 2 cut(s) 142, 675
KpnI GGTACC 1 cut(s) 551
Ksp22I TGATCA 2 cut(s) 583, 1690
Kzo9I GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
LmnI GCTCC 2 cut(s) 1085, 1310
Lsp1109I GCAGC 9 cut(s) 281, 303, 743, 931, 935, 1213, 1217, 1541, 1560
MaeI CTAG 4 cut(s) 735, 750, 1140, 1599
MaeIII GTNAC 5 cut(s) 5, 241, 799, 1105, 1255
MalI GATC 8 cut(s) 44, 72, 177, 386, 585, 1390, 1485, 1692
MboI GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
MfeI CAATTG 1 cut(s) 337
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 2 cut(s) 644, 1586
MlsI TGGCCA 3 cut(s) 1331, 1418, 1593
MluNI TGGCCA 3 cut(s) 1331, 1418, 1593
MlyI GAGTC 1 cut(s) 1343
MmeI TCCRAC 1 cut(s) 990
Mox20I TGGCCA 3 cut(s) 1331, 1418, 1593
MroXI GAANNNNTTC 1 cut(s) 744
MscI TGGCCA 3 cut(s) 1331, 1418, 1593
MseI TTAA 2 cut(s) 393, 978
MslI CAYNNNNRTG 3 cut(s) 228, 1122, 1599
Msp20I TGGCCA 3 cut(s) 1331, 1418, 1593
MspA1I CMGCKG 2 cut(s) 294, 1208
MspI CCGG 1 cut(s) 1358
MspR9I CCNGG 2 cut(s) 593, 805
MunI CAATTG 1 cut(s) 337
MvaI CCWGG 2 cut(s) 593, 805
MwoI GCNNNNNNNGC 8 cut(s) 303, 929, 1211, 1333, 1538, 1572, 1581, 1590
NcoI CCATGG 1 cut(s) 82
NdeI CATATG 1 cut(s) 934
NdeII GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
NlaIV GGNNCC 5 cut(s) 44, 267, 402, 549, 1722
NmuCI GTSAC 3 cut(s) 241, 799, 1105
NspI RCATGY 1 cut(s) 523
OliI CACNNNNGTG 2 cut(s) 228, 1599
PagI TCATGA 1 cut(s) 1501
PceI AGGCCT 1 cut(s) 715
PdmI GAANNNNTTC 1 cut(s) 744
PleI GAGTC 1 cut(s) 1343
PpsI GAGTC 1 cut(s) 1343
PshBI ATTAAT 1 cut(s) 978
Psp6I CCWGG 2 cut(s) 591, 803
PspGI CCWGG 2 cut(s) 591, 803
PspN4I GGNNCC 5 cut(s) 44, 267, 402, 549, 1722
PspPI GGNCC 4 cut(s) 266, 400, 538, 832
PstI CTGCAG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 42
PvuII CAGCTG 2 cut(s) 294, 1208
RsaI GTAC 7 cut(s) 349, 484, 524, 549, 994, 1097, 1175
RsaNI GTAC 7 cut(s) 348, 483, 523, 548, 993, 1096, 1174
RseI CAYNNNNRTG 3 cut(s) 228, 1122, 1599
SaqAI TTAA 2 cut(s) 393, 978
Sau3AI GATC 8 cut(s) 42, 70, 175, 384, 583, 1388, 1483, 1690
Sau96I GGNCC 4 cut(s) 266, 400, 538, 832
ScaI AGTACT 1 cut(s) 994
SchI GAGTC 1 cut(s) 1343
ScrFI CCNGG 2 cut(s) 593, 805
SduI GDGCHC 2 cut(s) 644, 1586
SfcI CTRYAG 1 cut(s) 729
SinI GGWCC 1 cut(s) 266
SmiMI CAYNNNNRTG 3 cut(s) 228, 1122, 1599
SmlI CTYRAG 4 cut(s) 37, 301, 950, 1300
SmoI CTYRAG 4 cut(s) 37, 301, 950, 1300
SpeI ACTAGT 1 cut(s) 1598
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 1 cut(s) 1361
SspMI CTAG 4 cut(s) 735, 750, 1140, 1599
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 2 cut(s) 591, 803
StyI CCWWGG 2 cut(s) 82, 1612
TaaI ACNGT 4 cut(s) 547, 552, 1437, 1469
TaqI TCGA 1 cut(s) 1368
TatI WGTACW 5 cut(s) 482, 522, 992, 1095, 1173
TauI GCSGC 1 cut(s) 1364
Tru1I TTAA 2 cut(s) 393, 978
Tru9I TTAA 2 cut(s) 393, 978
TscAI CASTG 2 cut(s) 1075, 1327
TseFI GTSAC 3 cut(s) 241, 799, 1105
TseI GCWGC 9 cut(s) 291, 294, 731, 923, 944, 1205, 1226, 1529, 1548
Tsp45I GTSAC 3 cut(s) 241, 799, 1105
TspDTI ATGAA 7 cut(s) 63, 231, 273, 576, 602, 1034, 1680
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 2 cut(s) 1075, 1327
VpaK11BI GGWCC 1 cut(s) 266
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 3 cut(s) 1040, 1293, 1507
XceI RCATGY 1 cut(s) 523
XcmI CCANNNNNNNNNTGG 2 cut(s) 53, 596
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 4 cut(s) 735, 750, 1140, 1599
ZrmI AGTACT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.