Rh2CG431400

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0010758
Physical Location & Seq
Forward (+)
58659235 .. 58660311
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG431400.1

Sequence Viewer

Length: 885 bp
ATGAGTAATTGGCTGGGATTCTCTCTCACTCCCCACCTGAGAATCCATGAAGGCTTTGATGGAAGAGAAGAAGATCAGCAAGAACAAGACCATGGAGTTGTGGGTTTCCCTCCTCACATGTCAGTCATGCCTTTGCGCTCTGATGGTTCTCTATGCTTTGCTGCTGACCCTTTCAGGCGTCCTAATGGTTCTGAAGATTGGAGGTCTTATGAGAATAGCATGGTGGGTGCAACAAGTGTGACAAACCCAGTTGATGATGATGAAGAGGGTCCTAAGCTCGAGGATTTCTTGGGAAGTTGCTACTCAAACTCACCACCTGGTGATGAAACCAAAGCTTACTGCCAAAACCAAGAGGACTCTAAAATTAATGTCAATGAGGCCCCAAATTTCAGCAACCCTAATAGTGAAATAGAAGCCAATTCCACAGACCCATCTCATCATCAGAGCTTGATTCAGCCTTACAGCCATTACTATGCAAACCCACAAAGTGGCGGTGCCCTTGTCCCGTGTACCAATGGCATGTACAAGTCTTGGTTAGGCCAAACTGTAGTGGTTCCATACGCTGATGTGAAATCACCATCTGATCAGGAAAATGGTAGCAATAACTTTCAGTCTAATCTCTCACTGACTATGAGTCCGACCTTGCAAAATGGTGGTATGCTTCCAATTTCTCAAGTGCAAATGGTGGAGAGCAAGAAAAGGCCAGGTGGGAAGTCCTCCGCAAGAGAGCCAGTCACTCGCAAATCTATTGATACTTTTGGGCAGAGAACTTCTCAGTATCGTGGTGTAACAAGGCATAGATGGACTGGGAGATACGAGGCCCATCTGTGGGATAACAGTTGCAGAAAAGAAGGACAAACAAGGAAAGGAAGGCAAGGTAATTAG

Protein Analysis

294

Amino Acids

32.72

Weight (kDa)

5.61

Isoelectric Point (pI)

60.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72570
fragaria_vesca FvH4_6g34710
malus_domestica MD00G1092000.v1.1 MD09G1183700.v1.1
prunus_persica Prupe.3G042000_v2.0.a1
pyrus_communis pycom09g10110
rosa_chinensis RchiOBHm_Chr2g0145271
rosa_laevigata RLG00000020135
rosa_multiflora Rmu_sc0001298.1_g000029
rosa_roxburghii Rroxscaffold_2G00100770
rosa_rugosa Rorug02G0391600
rosa_samantha Rh2AG444400 Rh2AG444500 Rh2BG455400 Rh2BG455500 Rh2CG431400 Rh2CG431500 Rh2DG465400 Rh2DG465500
rosa_wichuraiana Rw2G036320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AccB7I CCANNNNNTGG 1 cut(s) 488
AciI CCGC 2 cut(s) 492, 720
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 178
AdeI CACNNNGTG 2 cut(s) 320, 488
AfaI GTAC 2 cut(s) 511, 524
AfiI CCNNNNNNNGG 3 cut(s) 488, 828, 829
AflIII ACRYGT 1 cut(s) 117
AhdI GACNNNNNGTC 1 cut(s) 633
AjnI CCWGG 2 cut(s) 316, 703
AluBI AGCT 3 cut(s) 277, 335, 447
AluI AGCT 3 cut(s) 277, 335, 447
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 4 cut(s) 378, 538, 701, 819
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 385
AseI ATTAAT 1 cut(s) 366
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 3 cut(s) 269, 379, 820
AsuHPI GGTGA 3 cut(s) 303, 332, 567
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 494
BbvI GCAGC 1 cut(s) 148
BccI CCATC 6 cut(s) 53, 137, 439, 586, 795, 831
BciT130I CCWGG 2 cut(s) 318, 705
BclI TGATCA 1 cut(s) 583
BfmI CTRYAG 1 cut(s) 546
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 2 cut(s) 318, 705
Bme18I GGWCC 1 cut(s) 269
BmeRI GACNNNNNGTC 1 cut(s) 633
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 269, 379, 820
BmiI GGNNCC 4 cut(s) 270, 381, 496, 555
BmrFI CCNGG 2 cut(s) 318, 705
BmrI ACTGGG 2 cut(s) 242, 816
BmuI ACTGGG 2 cut(s) 242, 816
BplI GAGNNNNNCTC 2 cut(s) 757, 789
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 3 cut(s) 488, 828, 829
Bse1I ACTGG 3 cut(s) 248, 731, 811
BseBI CCWGG 2 cut(s) 318, 705
BseDI CCNNGG 1 cut(s) 91
BseLI CCNNNNNNNGG 3 cut(s) 488, 828, 829
BseMII CTCAG 2 cut(s) 29, 788
BseNI ACTGG 3 cut(s) 248, 731, 811
BseRI GAGGAG 1 cut(s) 102
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 1 cut(s) 148
BseYI CCCAGC 1 cut(s) 13
BshFI GGCC 4 cut(s) 380, 540, 703, 821
BshNI GGYRCC 1 cut(s) 494
BsiHKCI CYCGRG 1 cut(s) 278
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 3 cut(s) 488, 828, 829
BsmFI GGGAC 1 cut(s) 488
BsnI GGCC 4 cut(s) 380, 540, 703, 821
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 499
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 2 cut(s) 73, 583
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 492, 720
BspANI GGCC 4 cut(s) 380, 540, 703, 821
BspCNI CTCAG 2 cut(s) 30, 787
BspLI GGNNCC 4 cut(s) 270, 381, 496, 555
BspT107I GGYRCC 1 cut(s) 494
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 3 cut(s) 248, 731, 811
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 2 cut(s) 73, 583
BssNI GRCGYC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 2 cut(s) 318, 705
Bst4CI ACNGT 2 cut(s) 547, 839
Bst6I CTCTTC 2 cut(s) 58, 258
BstACI GRCGYC 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 522
BstDEI CTNAG 3 cut(s) 38, 273, 774
BstDSI CCRYGG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 2 cut(s) 76, 586
BstMBI GATC 2 cut(s) 73, 583
BstNI CCWGG 2 cut(s) 318, 705
BstNSI RCATGY 2 cut(s) 121, 523
BstSCI CCNGG 2 cut(s) 316, 703
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 499
BstV1I GCAGC 1 cut(s) 148
BsuRI GGCC 4 cut(s) 380, 540, 703, 821
BtgI CCRYGG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 623
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 3 cut(s) 269, 379, 820
CseI GACGC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 316
Csp6I GTAC 2 cut(s) 510, 523
CviAII CATG 6 cut(s) 47, 92, 118, 127, 220, 520
CviQI GTAC 2 cut(s) 510, 523
DdeI CTNAG 3 cut(s) 38, 273, 774
DpnI GATC 2 cut(s) 75, 585
DpnII GATC 2 cut(s) 73, 583
DraIII CACNNNGTG 2 cut(s) 320, 488
DriI GACNNNNNGTC 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 58, 258
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 2 cut(s) 58, 258
Eco130I CCWWGG 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 278
EcoO109I RGGNCCY 2 cut(s) 269, 379
EcoRII CCWGG 2 cut(s) 316, 703
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 6 cut(s) 50, 95, 121, 130, 223, 523
FaqI GGGAC 1 cut(s) 488
FatI CATG 6 cut(s) 46, 91, 117, 126, 219, 519
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 162
GlaI GCGC 1 cut(s) 137
GluI GCNGC 1 cut(s) 162
GsaI CCCAGC 1 cut(s) 17
HaeIII GGCC 4 cut(s) 380, 540, 703, 821
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 6 cut(s) 50, 95, 121, 130, 223, 523
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 333
HinfI GANTC 5 cut(s) 18, 42, 356, 451, 634
HphI GGTGA 3 cut(s) 303, 332, 567
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 5 cut(s) 142, 193, 444, 583, 639
Hpy188III TCNNGA 1 cut(s) 587
Hpy8I GTNNAC 1 cut(s) 510
HpyAV CCTTC 3 cut(s) 44, 845, 864
HpyCH4III ACNGT 2 cut(s) 547, 839
HpyCH4V TGCA 5 cut(s) 230, 476, 646, 679, 843
HpyF3I CTNAG 3 cut(s) 38, 273, 774
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 6 cut(s) 50, 95, 121, 130, 223, 523
HspAI GCGC 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 2 cut(s) 73, 583
Lsp1109I GCAGC 1 cut(s) 148
MabI ACCWGGT 1 cut(s) 316
MaeIII GTNAC 3 cut(s) 238, 733, 787
MalI GATC 2 cut(s) 75, 585
MboI GATC 2 cut(s) 73, 583
MboII GAAGA 5 cut(s) 75, 80, 83, 206, 275
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 6 cut(s) 7, 363, 385, 418, 666, 880
MlyI GAGTC 2 cut(s) 350, 643
MmeI TCCRAC 1 cut(s) 662
MnlI CCTC 9 cut(s) 120, 123, 195, 259, 274, 346, 370, 727, 811
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 471
MspR9I CCNGG 2 cut(s) 318, 705
MvaI CCWGG 2 cut(s) 318, 705
NcoI CCATGG 1 cut(s) 91
NdeII GATC 2 cut(s) 73, 583
NlaIII CATG 6 cut(s) 50, 95, 121, 130, 223, 523
NlaIV GGNNCC 4 cut(s) 270, 381, 496, 555
NmuCI GTSAC 2 cut(s) 238, 733
NspI RCATGY 2 cut(s) 121, 523
PaeR7I CTCGAG 1 cut(s) 278
PciI ACATGT 1 cut(s) 117
PfeI GAWTC 3 cut(s) 18, 42, 451
PflMI CCANNNNNTGG 1 cut(s) 488
PkrI GCNGC 1 cut(s) 163
PleI GAGTC 2 cut(s) 350, 642
PpsI GAGTC 2 cut(s) 350, 642
PpuMI RGGWCCY 1 cut(s) 269
PscI ACATGT 1 cut(s) 117
PshBI ATTAAT 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 269
Psp6I CCWGG 2 cut(s) 316, 703
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 2 cut(s) 316, 703
PspN4I GGNNCC 4 cut(s) 270, 381, 496, 555
PspPI GGNCC 3 cut(s) 269, 379, 820
PspPPI RGGWCCY 1 cut(s) 269
PspXI VCTCGAGB 1 cut(s) 278
RsaI GTAC 2 cut(s) 511, 524
RsaNI GTAC 2 cut(s) 510, 523
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 2 cut(s) 73, 583
Sau96I GGNCC 3 cut(s) 269, 379, 820
SchI GAGTC 2 cut(s) 350, 643
ScrFI CCNGG 2 cut(s) 318, 705
SduI GDGCHC 1 cut(s) 499
SetI ASST 9 cut(s) 39, 206, 279, 319, 337, 449, 644, 709, 880
SexAI ACCWGGT 1 cut(s) 316
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 2 cut(s) 278, 672
SmoI CTYRAG 2 cut(s) 278, 672
Sse9I AATT 6 cut(s) 7, 363, 385, 418, 666, 880
SsiI CCGC 2 cut(s) 492, 720
StyD4I CCNGG 2 cut(s) 316, 703
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 547, 839
TaqI TCGA 1 cut(s) 279
TasI AATT 6 cut(s) 7, 363, 385, 418, 666, 880
TatI WGTACW 1 cut(s) 522
TfiI GAWTC 3 cut(s) 18, 42, 451
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 630
TseFI GTSAC 2 cut(s) 238, 733
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 2 cut(s) 238, 733
TspDTI ATGAA 3 cut(s) 63, 276, 339
TspRI CASTG 1 cut(s) 630
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 269
VspI ATTAAT 1 cut(s) 366
XapI RAATTY 1 cut(s) 385
XceI RCATGY 2 cut(s) 121, 523
XhoI CTCGAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.