RLG00000020135

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010758
Physical Location & Seq
Forward (+)
61483661 .. 61486465
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020135

Sequence Viewer

Length: 1680 bp
ATGAGTAATTGGCTAGGATTCTCTCTCGCTCCCCACCTGAGAATCCATGAAGGCTTTGATGGAAGAGAAGAAGATCAGCAAGAACAAGACCATGGAGTTGTGGGTTTCCCTCCTCACATGTCAGTCATGCCTCTGCGGTCTGATGGTTCTCTTTGCTTTGCTGCTGACCCTTTCAGGCGTCCTAATGGTTCTGAAGATTGGAGGTCTTATGAGAATAGCATGGTGGGTGCAACAAGTGTTACAAACCCAGTTGATGATGATGAGGAGGGTCCTAAGCTCGAGGATTTCTTGGGAAGTTGCTACTCAAACTCACCACCTGGTGATGAAACCAAAGTTTACTGCCAAAACCAAGAGGACTCTAAAATTAATGTCAATGAGGCCCCAAATTTCAGCAACCCTAATAGTGAAATAGAAGCCAATTCCACAGACCCATCTCATCATCAGAGCTTGATTCAGCCTTACAGCCATTATTATGCAAACCCACAAAGTGGCGGTGCTCTTGTCCCATGTACCAATGGCATGTACAAGTCTTGGTTAGGCCAAACTGTAGTGGTTCCATACGCTGATGTGAAATCACCATCTGATCAGGAAAATGGTCGCAATAACTTTCAGTCTAATCTCTCACTGACTATGAGTCCGACCTTGCAAAATGGTGGTATGCTTCCAATTTCTCAAGTGCAAATGGTGGAGAGCAAGAAAAGGCCAGGTGGGAAGTCCTCCGCAAGAGAACCAGTCACTCGCAAATCTATTGATACTTTTGGGCAGAGAACTTCTCAGTATCGTGGTGTAACAAGTTGCAGAAAAGAAGGACAAACAAGGAAAGGAAGGCAAGGTGGATATGATAAGGAAGAAAGAGCAGCAAAGGCTTATGATTTAGCTGCACTCAAGTATTGGGGTCCAACTACTCATATTAACTTCCCCATAAGCACTTATGAGAAAGAGCTGGAAGAGATGAAGACCATGACAAGGCAAGAATTTGTAGCCAACTTGAGGAGGAAAAGCAGTGGGTTTTCAAGGGGAGCTTCTGTGTACAGAGGAGTGACAAGACACCATCAACATGGAAGGTGGCAAGCTAGAATTGGAAGGGTTGCTGGAAACAAGGATTTGTACCTTGGAACATTTAGCACACAAGAAGAAGCAGCTGAGGCTTATGACATAGCAGCAATCAAGTTTAGGGGCACAAGTGCTGTGACCAACTTTGACATGAGTAGGTATGATGTAAAGAGGATTTGCTCAAGCTCCACACTCATTACAAGTGACCTGGCCAAACGCCCATCACCAAAAGACCCGGCTCCAGCAGCACCAAATGATCAGGAGTTCAATTCTTGTGCTTCGTCTACTTCTTCACAACCCCTTTTGGCCATAACAAACAGCGAAGAACCTTGTGATGAGAATTTGGCTGAAATGGTGTGGAATGACAATCCAAACTCTGAGGCCAACACTGTGACATTGGCTGCTGCTTCAGGCAGCAGGAATTCTTCTTGCCCACAAAGCCCTAAATGCTGCGAATTTGGATCCAACAGTGAGTTTGGTCTCAGTGTAGGTGACTATTCTCATGCTTATTTCAGCTCTCTGCAGGGTGCTAAGTATGAAGATGGCAATGGTGGTAGTGACCACAGCCGGCTTGGGAATCTTGGGTTGGTTCAACAGGTCCCTATGTTTGCATTGTGGAATGATTGA

Protein Analysis

560

Amino Acids

61.51

Weight (kDa)

5.56

Isoelectric Point (pI)

57.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 342 - 393 2.8e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72570
fragaria_vesca FvH4_6g34710
malus_domestica MD00G1092000.v1.1 MD09G1183700.v1.1
prunus_persica Prupe.3G042000_v2.0.a1
pyrus_communis pycom09g10110
rosa_chinensis RchiOBHm_Chr2g0145271
rosa_laevigata RLG00000020135
rosa_multiflora Rmu_sc0001298.1_g000029
rosa_roxburghii Rroxscaffold_2G00100770
rosa_rugosa Rorug02G0391600
rosa_samantha Rh2AG444400 Rh2AG444500 Rh2BG455400 Rh2BG455500 Rh2CG431400 Rh2CG431500 Rh2DG465400 Rh2DG465500
rosa_wichuraiana Rw2G036320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 488
AccI GTMKAC 1 cut(s) 1337
AciI CCGC 3 cut(s) 136, 492, 720
AclWI GGATC 2 cut(s) 1509, 1522
AcoI YGGCCR 2 cut(s) 1263, 1359
AcsI RAATTY 5 cut(s) 385, 974, 1393, 1474, 1508
AcuI CTGAAG 2 cut(s) 213, 1446
AcyI GRCGYC 1 cut(s) 178
AdeI CACNNNGTG 2 cut(s) 320, 488
AfaI GTAC 4 cut(s) 511, 524, 1031, 1109
AfiI CCNNNNNNNGG 3 cut(s) 488, 966, 990
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 3 cut(s) 1014, 1321, 1646
AhdI GACNNNNNGTC 1 cut(s) 633
AjnI CCWGG 3 cut(s) 316, 703, 1260
AjuI GAANNNNNNNTTGG 2 cut(s) 1622, 1654
AluBI AGCT 9 cut(s) 277, 447, 878, 943, 1022, 1073, 1142, 1239, 1569
AluI AGCT 9 cut(s) 277, 447, 878, 943, 1022, 1073, 1142, 1239, 1569
Alw21I GWGCWC 1 cut(s) 499
Alw26I GTCTC 1 cut(s) 1538
AlwI GGATC 2 cut(s) 1509, 1522
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 6 cut(s) 378, 538, 701, 1263, 1359, 1434
ApoI RAATTY 5 cut(s) 385, 974, 1393, 1474, 1508
AseI ATTAAT 1 cut(s) 366
AspS9I GGNCC 4 cut(s) 269, 379, 896, 1651
AsuC2I CCSGG 1 cut(s) 1289
AsuHPI GGTGA 5 cut(s) 303, 332, 567, 1269, 1556
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 3 cut(s) 269, 896, 1651
BaeGI GKGCMC 1 cut(s) 1181
BalI TGGCCA 2 cut(s) 1265, 1361
BamHI GGATCC 1 cut(s) 1514
BbsI GAAGAC 1 cut(s) 962
Bbv12I GWGCWC 1 cut(s) 499
BbvCI CCTCAGC 1 cut(s) 1143
BccI CCATC 7 cut(s) 53, 137, 439, 586, 1059, 1282, 1589
BciT130I CCWGG 3 cut(s) 318, 705, 1262
BclI TGATCA 2 cut(s) 583, 1309
BcnI CCSGG 1 cut(s) 1289
BcoDI GTCTC 1 cut(s) 1538
BfaI CTAG 2 cut(s) 14, 1074
BfmI CTRYAG 2 cut(s) 546, 1574
Bme1390I CCNGG 4 cut(s) 318, 705, 1262, 1289
Bme18I GGWCC 3 cut(s) 269, 896, 1651
BmeRI GACNNNNNGTC 1 cut(s) 633
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 4 cut(s) 269, 379, 896, 1651
BmiI GGNNCC 7 cut(s) 270, 381, 555, 897, 1293, 1516, 1653
BmrFI CCNGG 4 cut(s) 318, 705, 1262, 1289
BmrI ACTGGG 1 cut(s) 242
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 962
BplI GAGNNNNNCTC 2 cut(s) 757, 789
BpmI CTGGAG 1 cut(s) 1278
Bpu10I CCTNAGC 2 cut(s) 273, 1143
BpuEI CTTGAG 4 cut(s) 657, 869, 1009, 1219
BpuMI CCSGG 1 cut(s) 1289
BsaHI GRCGYC 1 cut(s) 178
BsaI GGTCTC 1 cut(s) 1538
BsaJI CCNNGG 2 cut(s) 91, 1111
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 3 cut(s) 488, 966, 990
Bse118I RCCGGY 1 cut(s) 1620
Bse1I ACTGG 2 cut(s) 248, 731
Bse3DI GCAATG 1 cut(s) 1606
BseBI CCWGG 3 cut(s) 318, 705, 1262
BseDI CCNNGG 2 cut(s) 91, 1111
BseLI CCNNNNNNNGG 3 cut(s) 488, 966, 990
BseMI GCAATG 1 cut(s) 1606
BseMII CTCAG 5 cut(s) 29, 788, 1134, 1422, 1549
BseNI ACTGG 2 cut(s) 248, 731
BseRI GAGGAG 4 cut(s) 102, 278, 1006, 1050
BseSI GKGCMC 1 cut(s) 1181
BsgI GTGCAG 1 cut(s) 864
BshFI GGCC 6 cut(s) 380, 540, 703, 1265, 1361, 1436
BsiHKAI GWGCWC 1 cut(s) 499
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 2 cut(s) 1289, 1621
BslFI GGGAC 2 cut(s) 488, 1637
BslI CCNNNNNNNGG 3 cut(s) 488, 966, 990
BsmAI GTCTC 1 cut(s) 1538
BsmFI GGGAC 2 cut(s) 488, 1637
BsnI GGCC 6 cut(s) 380, 540, 703, 1265, 1361, 1436
Bso31I GGTCTC 1 cut(s) 1538
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 499, 1181
Bsp1407I TGTACA 2 cut(s) 522, 1029
Bsp143I GATC 4 cut(s) 73, 583, 1309, 1514
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 3 cut(s) 136, 492, 720
BspANI GGCC 6 cut(s) 380, 540, 703, 1265, 1361, 1436
BspCNI CTCAG 5 cut(s) 30, 787, 1135, 1423, 1548
BspLI GGNNCC 7 cut(s) 270, 381, 555, 897, 1293, 1516, 1653
BspMAI CTGCAG 1 cut(s) 1578
BspPI GGATC 2 cut(s) 1509, 1522
BspTNI GGTCTC 1 cut(s) 1538
BsrDI GCAATG 1 cut(s) 1606
BsrFI RCCGGY 1 cut(s) 1620
BsrGI TGTACA 2 cut(s) 522, 1029
BsrI ACTGG 2 cut(s) 248, 731
BssAI RCCGGY 1 cut(s) 1620
BssECI CCNNGG 2 cut(s) 91, 1111
BssMI GATC 4 cut(s) 73, 583, 1309, 1514
BssNI GRCGYC 1 cut(s) 178
BssT1I CCWWGG 2 cut(s) 91, 1111
Bst2UI CCWGG 3 cut(s) 318, 705, 1262
Bst4CI ACNGT 3 cut(s) 547, 1444, 1523
Bst6I CTCTTC 2 cut(s) 58, 942
BstACI GRCGYC 1 cut(s) 178
BstAUI TGTACA 2 cut(s) 522, 1029
BstC8I GCNNGC 2 cut(s) 1071, 1622
BstDEI CTNAG 7 cut(s) 38, 273, 774, 1143, 1431, 1535, 1584
BstDSI CCRYGG 1 cut(s) 91
BstKTI GATC 4 cut(s) 76, 586, 1312, 1517
BstMAI GTCTC 1 cut(s) 1538
BstMBI GATC 4 cut(s) 73, 583, 1309, 1514
BstMWI GCNNNNNNNGC 5 cut(s) 863, 1145, 1298, 1491, 1500
BstNI CCWGG 3 cut(s) 318, 705, 1262
BstNSI RCATGY 2 cut(s) 121, 523
BstSCI CCNGG 4 cut(s) 316, 703, 1260, 1287
BstSFI CTRYAG 2 cut(s) 546, 1574
BstSLI GKGCMC 1 cut(s) 1181
BstV2I GAAGAC 1 cut(s) 962
BstX2I RGATCY 1 cut(s) 1514
BstXI CCANNNNNNTGG 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1514
BsuRI GGCC 6 cut(s) 380, 540, 703, 1265, 1361, 1436
BtgI CCRYGG 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 5 cut(s) 623, 1009, 1440, 1528, 1543
Cac8I GCNNGC 2 cut(s) 1071, 1622
Cfr10I RCCGGY 1 cut(s) 1620
Cfr13I GGNCC 4 cut(s) 269, 379, 896, 1651
CseI GACGC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 316
Csp6I GTAC 4 cut(s) 510, 523, 1030, 1108
CviQI GTAC 4 cut(s) 510, 523, 1030, 1108
DdeI CTNAG 7 cut(s) 38, 273, 774, 1143, 1431, 1535, 1584
DpnI GATC 4 cut(s) 75, 585, 1311, 1516
DpnII GATC 4 cut(s) 73, 583, 1309, 1514
DraIII CACNNNGTG 2 cut(s) 320, 488
DriI GACNNNNNGTC 1 cut(s) 633
EaeI YGGCCR 2 cut(s) 1263, 1359
Eam1104I CTCTTC 2 cut(s) 58, 942
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 2 cut(s) 58, 942
Eco130I CCWWGG 2 cut(s) 91, 1111
Eco31I GGTCTC 1 cut(s) 1538
Eco47I GGWCC 3 cut(s) 269, 896, 1651
Eco57I CTGAAG 2 cut(s) 213, 1446
Eco88I CYCGRG 1 cut(s) 278
EcoO109I RGGNCCY 3 cut(s) 269, 379, 1651
EcoRI GAATTC 1 cut(s) 1474
EcoRII CCWGG 3 cut(s) 316, 703, 1260
EcoT14I CCWWGG 2 cut(s) 91, 1111
ErhI CCWWGG 2 cut(s) 91, 1111
FalI AAGNNNNNCTT 2 cut(s) 1006, 1038
FaqI GGGAC 2 cut(s) 488, 1637
FbaI TGATCA 2 cut(s) 583, 1309
FblI GTMKAC 1 cut(s) 1337
FspBI CTAG 2 cut(s) 14, 1074
GsuI CTGGAG 1 cut(s) 1278
HaeIII GGCC 6 cut(s) 380, 540, 703, 1265, 1361, 1436
HapII CCGG 2 cut(s) 1289, 1621
HgaI GACGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 178
HinfI GANTC 6 cut(s) 18, 42, 356, 451, 634, 1630
HpaII CCGG 2 cut(s) 1289, 1621
HphI GGTGA 5 cut(s) 303, 332, 567, 1269, 1556
Hpy166II GTNNAC 3 cut(s) 337, 1030, 1338
Hpy188I TCNGA 6 cut(s) 142, 193, 444, 583, 639, 1432
Hpy188III TCNNGA 2 cut(s) 587, 1313
Hpy8I GTNNAC 3 cut(s) 337, 1030, 1338
HpyAV CCTTC 5 cut(s) 44, 800, 819, 1056, 1077
HpyCH4III ACNGT 3 cut(s) 547, 1444, 1523
HpyCH4V TGCA 8 cut(s) 230, 476, 646, 679, 798, 881, 1576, 1664
HpyF10VI GCNNNNNNNGC 5 cut(s) 863, 1145, 1298, 1491, 1500
HpyF3I CTNAG 7 cut(s) 38, 273, 774, 1143, 1431, 1535, 1584
Hsp92I GRCGYC 1 cut(s) 178
KroI GCCGGC 1 cut(s) 1620
KroNI GCCGGC 1 cut(s) 1622
Ksp22I TGATCA 2 cut(s) 583, 1309
Kzo9I GATC 4 cut(s) 73, 583, 1309, 1514
LmnI GCTCC 4 cut(s) 34, 1019, 1244, 1297
MabI ACCWGGT 1 cut(s) 316
MaeI CTAG 2 cut(s) 14, 1074
MaeIII GTNAC 9 cut(s) 238, 733, 787, 1039, 1189, 1256, 1444, 1544, 1610
MalI GATC 4 cut(s) 75, 585, 1311, 1516
MboI GATC 4 cut(s) 73, 583, 1309, 1514
MflI RGATCY 1 cut(s) 1514
MhlI GDGCHC 2 cut(s) 499, 1181
MlsI TGGCCA 2 cut(s) 1265, 1361
MluNI TGGCCA 2 cut(s) 1265, 1361
MlyI GAGTC 2 cut(s) 350, 643
MmeI TCCRAC 3 cut(s) 662, 923, 1542
Mox20I TGGCCA 2 cut(s) 1265, 1361
MroNI GCCGGC 1 cut(s) 1620
MscI TGGCCA 2 cut(s) 1265, 1361
MseI TTAA 2 cut(s) 366, 912
MslI CAYNNNNRTG 2 cut(s) 471, 1056
Msp20I TGGCCA 2 cut(s) 1265, 1361
MspA1I CMGCKG 1 cut(s) 1142
MspI CCGG 2 cut(s) 1289, 1621
MspR9I CCNGG 4 cut(s) 318, 705, 1262, 1289
MvaI CCWGG 3 cut(s) 318, 705, 1262
MwoI GCNNNNNNNGC 5 cut(s) 863, 1145, 1298, 1491, 1500
NaeI GCCGGC 1 cut(s) 1622
NciI CCSGG 1 cut(s) 1289
NcoI CCATGG 1 cut(s) 91
NdeII GATC 4 cut(s) 73, 583, 1309, 1514
NgoMIV GCCGGC 1 cut(s) 1620
NlaIV GGNNCC 7 cut(s) 270, 381, 555, 897, 1293, 1516, 1653
NmuCI GTSAC 7 cut(s) 733, 1039, 1189, 1256, 1444, 1544, 1610
NspI RCATGY 2 cut(s) 121, 523
PaeR7I CTCGAG 1 cut(s) 278
PciI ACATGT 1 cut(s) 117
PdiI GCCGGC 1 cut(s) 1622
PfeI GAWTC 4 cut(s) 18, 42, 451, 1630
PflMI CCANNNNNTGG 1 cut(s) 488
PleI GAGTC 2 cut(s) 350, 642
PpsI GAGTC 2 cut(s) 350, 642
PpuMI RGGWCCY 2 cut(s) 269, 1651
PscI ACATGT 1 cut(s) 117
PshBI ATTAAT 1 cut(s) 366
Psp5II RGGWCCY 2 cut(s) 269, 1651
Psp6I CCWGG 3 cut(s) 316, 703, 1260
PspGI CCWGG 3 cut(s) 316, 703, 1260
PspN4I GGNNCC 7 cut(s) 270, 381, 555, 897, 1293, 1516, 1653
PspPI GGNCC 4 cut(s) 269, 379, 896, 1651
PspPPI RGGWCCY 2 cut(s) 269, 1651
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 1 cut(s) 1578
PsuI RGATCY 1 cut(s) 1514
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 4 cut(s) 511, 524, 1031, 1109
RsaNI GTAC 4 cut(s) 510, 523, 1030, 1108
RseI CAYNNNNRTG 2 cut(s) 471, 1056
SaqAI TTAA 2 cut(s) 366, 912
Sau3AI GATC 4 cut(s) 73, 583, 1309, 1514
Sau96I GGNCC 4 cut(s) 269, 379, 896, 1651
SchI GAGTC 2 cut(s) 350, 643
ScrFI CCNGG 4 cut(s) 318, 705, 1262, 1289
SduI GDGCHC 2 cut(s) 499, 1181
SexAI ACCWGGT 1 cut(s) 316
SfcI CTRYAG 2 cut(s) 546, 1574
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 3 cut(s) 269, 896, 1651
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 2 cut(s) 471, 1056
SmlI CTYRAG 5 cut(s) 278, 672, 884, 988, 1234
SmoI CTYRAG 5 cut(s) 278, 672, 884, 988, 1234
SsiI CCGC 3 cut(s) 136, 492, 720
SspMI CTAG 2 cut(s) 14, 1074
StyD4I CCNGG 4 cut(s) 316, 703, 1260, 1287
StyI CCWWGG 2 cut(s) 91, 1111
TaaI ACNGT 3 cut(s) 547, 1444, 1523
TaqI TCGA 1 cut(s) 279
TatI WGTACW 2 cut(s) 522, 1029
TfiI GAWTC 4 cut(s) 18, 42, 451, 1630
Tru1I TTAA 2 cut(s) 366, 912
Tru9I TTAA 2 cut(s) 366, 912
TscAI CASTG 5 cut(s) 630, 1009, 1447, 1528, 1543
TseFI GTSAC 7 cut(s) 733, 1039, 1189, 1256, 1444, 1544, 1610
Tsp45I GTSAC 7 cut(s) 733, 1039, 1189, 1256, 1444, 1544, 1610
TspDTI ATGAA 4 cut(s) 63, 339, 968, 1605
TspRI CASTG 5 cut(s) 630, 1009, 1447, 1528, 1543
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 269, 896, 1651
VspI ATTAAT 1 cut(s) 366
XapI RAATTY 5 cut(s) 385, 974, 1393, 1474, 1508
XceI RCATGY 2 cut(s) 121, 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 1622
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 1337
XspI CTAG 2 cut(s) 14, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.