Rh2DG465500

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010758
Physical Location & Seq
Forward (+)
67398684 .. 67399666
983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG465500.1

Sequence Viewer

Length: 711 bp
ATGTGGTTCTTTATTATGTTCTACAGGAAAAGCAGTGGGTTTTCAAGGGGAGCTTCTGTGTACAGAGGAGTGACAAGACACCATCAACATGGAAGGTGGCAAGCTAGAATTGGAAGGGTTGCTGGAAACAAGGATTTGTACCTTGGAACATTTAGCACACAAGAAGAAGCAGCTGAGGCTTATGACATAGCAGCAATCAAGTTTAGGGGCACAAGTGCTGTGACCAACTTTGACATGAGTAGGTATGATGTAAAGAGGATTTGCTCAAGCTCCACACTCATTACAAGTGACCTGGCCAAACGCCCGTCACCAAAAGACCCGGCTCCAGCAGCACCAAATGATCAGGAGTTCAATTCTTGTGCTTCATCTACTTCTTCACAACCCCTTTTGGCCATAACAAACAGCGAAGAACCTTGTGATGAGAATTTGGCTGAAATGGTGTGGAATGACAATCCAAACTCTGAGGCCAACACTGTGACATTGGCTGCTGCTTCAGGCAGCAGGAATTCTTCTTGCCCTCAAAGCCCTAAATGCTGCGAATTTGGATCCAACAGTGAGTTTGGTCTCAGTGTAGGTGACTATTCTCATGCTTATTTCAGCTCTCTGCAGGGTGCTAAGTATGAAGATGGCAATGGTGGTAGTGACCACAGCCGGCTTGGGAATCTTGGGTTGGTTCAACAGGTCCCTATGTTTGCATTGTGGAATGATTGA

Protein Analysis

236

Amino Acids

25.76

Weight (kDa)

5.74

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 19 - 70 7.8e-10 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72570
fragaria_vesca FvH4_6g34710
malus_domestica MD00G1092000.v1.1 MD09G1183700.v1.1
prunus_persica Prupe.3G042000_v2.0.a1
pyrus_communis pycom09g10110
rosa_chinensis RchiOBHm_Chr2g0145271
rosa_laevigata RLG00000020135
rosa_multiflora Rmu_sc0001298.1_g000029
rosa_roxburghii Rroxscaffold_2G00100770
rosa_rugosa Rorug02G0391600
rosa_samantha Rh2AG444400 Rh2AG444500 Rh2BG455400 Rh2BG455500 Rh2CG431400 Rh2CG431500 Rh2DG465400 Rh2DG465500
rosa_wichuraiana Rw2G036320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 540, 553
AcoI YGGCCR 2 cut(s) 294, 390
AcsI RAATTY 3 cut(s) 424, 505, 539
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 2 cut(s) 62, 140
AgsI TTSAA 3 cut(s) 45, 352, 677
AjnI CCWGG 1 cut(s) 291
AjuI GAANNNNNNNTTGG 2 cut(s) 653, 685
AluBI AGCT 5 cut(s) 53, 104, 173, 270, 600
AluI AGCT 5 cut(s) 53, 104, 173, 270, 600
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 2 cut(s) 540, 553
AoxI GGCC 3 cut(s) 294, 390, 465
ApeKI GCWGC 7 cut(s) 170, 191, 329, 485, 488, 498, 534
ApoI RAATTY 3 cut(s) 424, 505, 539
AspS9I GGNCC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 300, 587
AvaII GGWCC 1 cut(s) 682
BaeGI GKGCMC 1 cut(s) 212
BalI TGGCCA 2 cut(s) 296, 392
BamHI GGATCC 1 cut(s) 545
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 7 cut(s) 182, 203, 341, 472, 475, 510, 521
BccI CCATC 2 cut(s) 90, 620
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 2 cut(s) 22, 605
BisI GCNGC 7 cut(s) 171, 192, 330, 486, 489, 499, 535
BlsI GCNGC 7 cut(s) 172, 193, 331, 487, 490, 500, 536
Bme1390I CCNGG 2 cut(s) 293, 320
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 3 cut(s) 324, 547, 684
BmrFI CCNGG 2 cut(s) 293, 320
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 1 cut(s) 320
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bse118I RCCGGY 1 cut(s) 651
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 3 cut(s) 165, 453, 580
BseRI GAGGAG 1 cut(s) 81
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 7 cut(s) 182, 203, 341, 472, 475, 510, 521
BshFI GGCC 3 cut(s) 296, 392, 467
BsiSI CCGG 2 cut(s) 320, 652
BslFI GGGAC 1 cut(s) 668
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 3 cut(s) 296, 392, 467
Bso31I GGTCTC 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 2 cut(s) 340, 545
BspANI GGCC 3 cut(s) 296, 392, 467
BspCNI CTCAG 3 cut(s) 166, 454, 579
BspLI GGNNCC 3 cut(s) 324, 547, 684
BspMAI CTGCAG 1 cut(s) 609
BspPI GGATC 2 cut(s) 540, 553
BspTNI GGTCTC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 651
BsrGI TGTACA 1 cut(s) 60
BssAI RCCGGY 1 cut(s) 651
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 340, 545
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 475, 554
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 102, 653
BstDEI CTNAG 4 cut(s) 174, 462, 566, 615
BstKTI GATC 2 cut(s) 343, 548
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 2 cut(s) 340, 545
BstMWI GCNNNNNNNGC 4 cut(s) 176, 329, 522, 531
BstNI CCWGG 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 291, 318
BstSFI CTRYAG 2 cut(s) 22, 605
BstSLI GKGCMC 1 cut(s) 212
BstV1I GCAGC 7 cut(s) 182, 203, 341, 472, 475, 510, 521
BstX2I RGATCY 1 cut(s) 545
BstXI CCANNNNNNTGG 1 cut(s) 89
BstYI RGATCY 1 cut(s) 545
BsuRI GGCC 3 cut(s) 296, 392, 467
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 4 cut(s) 40, 471, 559, 574
Cac8I GCNNGC 2 cut(s) 102, 653
Cfr10I RCCGGY 1 cut(s) 651
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 2 cut(s) 61, 139
CviAII CATG 3 cut(s) 89, 235, 587
CviQI GTAC 2 cut(s) 61, 139
DdeI CTNAG 4 cut(s) 174, 462, 566, 615
DpnI GATC 2 cut(s) 342, 547
DpnII GATC 2 cut(s) 340, 545
EaeI YGGCCR 2 cut(s) 294, 390
Eco130I CCWWGG 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 477
EcoO109I RGGNCCY 1 cut(s) 682
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 3 cut(s) 92, 238, 590
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 88, 234, 586
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 7 cut(s) 171, 192, 330, 486, 489, 499, 535
Fsp4HI GCNGC 7 cut(s) 171, 192, 330, 486, 489, 499, 535
FspBI CTAG 1 cut(s) 105
GluI GCNGC 7 cut(s) 171, 192, 330, 486, 489, 499, 535
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 3 cut(s) 296, 392, 467
HapII CCGG 2 cut(s) 320, 652
Hin1II CATG 3 cut(s) 92, 238, 590
HinfI GANTC 1 cut(s) 661
HpaII CCGG 2 cut(s) 320, 652
HphI GGTGA 2 cut(s) 300, 587
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 2 cut(s) 87, 108
HpyCH4III ACNGT 2 cut(s) 475, 554
HpyCH4V TGCA 2 cut(s) 607, 695
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 329, 522, 531
HpyF3I CTNAG 4 cut(s) 174, 462, 566, 615
Hsp92II CATG 3 cut(s) 92, 238, 590
KroI GCCGGC 1 cut(s) 651
KroNI GCCGGC 1 cut(s) 653
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 2 cut(s) 340, 545
LmnI GCTCC 3 cut(s) 50, 275, 328
Lsp1109I GCAGC 7 cut(s) 182, 203, 341, 472, 475, 510, 521
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 7 cut(s) 70, 220, 287, 306, 475, 575, 641
MalI GATC 2 cut(s) 342, 547
MboI GATC 2 cut(s) 340, 545
MboII GAAGA 5 cut(s) 176, 366, 419, 501, 635
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 1 cut(s) 212
MlsI TGGCCA 2 cut(s) 296, 392
MluCI AATT 5 cut(s) 108, 352, 424, 505, 539
MluNI TGGCCA 2 cut(s) 296, 392
MmeI TCCRAC 1 cut(s) 573
MnlI CCTC 5 cut(s) 59, 169, 249, 457, 528
Mox20I TGGCCA 2 cut(s) 296, 392
MroNI GCCGGC 1 cut(s) 651
MscI TGGCCA 2 cut(s) 296, 392
MslI CAYNNNNRTG 1 cut(s) 87
Msp20I TGGCCA 2 cut(s) 296, 392
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 320, 652
MspR9I CCNGG 2 cut(s) 293, 320
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 4 cut(s) 176, 329, 522, 531
NaeI GCCGGC 1 cut(s) 653
NciI CCSGG 1 cut(s) 320
NdeII GATC 2 cut(s) 340, 545
NgoMIV GCCGGC 1 cut(s) 651
NlaIII CATG 3 cut(s) 92, 238, 590
NlaIV GGNNCC 3 cut(s) 324, 547, 684
NmuCI GTSAC 7 cut(s) 70, 220, 287, 306, 475, 575, 641
PdiI GCCGGC 1 cut(s) 653
PfeI GAWTC 1 cut(s) 661
PkrI GCNGC 7 cut(s) 172, 193, 331, 487, 490, 500, 536
PpuMI RGGWCCY 1 cut(s) 682
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 3 cut(s) 324, 547, 684
PspPI GGNCC 1 cut(s) 682
PspPPI RGGWCCY 1 cut(s) 682
PstI CTGCAG 1 cut(s) 609
PsuI RGATCY 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 2 cut(s) 62, 140
RsaNI GTAC 2 cut(s) 61, 139
RseI CAYNNNNRTG 1 cut(s) 87
SatI GCNGC 7 cut(s) 171, 192, 330, 486, 489, 499, 535
Sau3AI GATC 2 cut(s) 340, 545
Sau96I GGNCC 1 cut(s) 682
ScrFI CCNGG 2 cut(s) 293, 320
SduI GDGCHC 1 cut(s) 212
SfcI CTRYAG 2 cut(s) 22, 605
SinI GGWCC 1 cut(s) 682
SmiMI CAYNNNNRTG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 5 cut(s) 108, 352, 424, 505, 539
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 2 cut(s) 291, 318
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 475, 554
TasI AATT 5 cut(s) 108, 352, 424, 505, 539
TatI WGTACW 1 cut(s) 60
TfiI GAWTC 1 cut(s) 661
TscAI CASTG 4 cut(s) 40, 478, 559, 574
TseFI GTSAC 7 cut(s) 70, 220, 287, 306, 475, 575, 641
TseI GCWGC 7 cut(s) 170, 191, 329, 485, 488, 498, 534
Tsp45I GTSAC 7 cut(s) 70, 220, 287, 306, 475, 575, 641
TspDTI ATGAA 2 cut(s) 354, 636
TspRI CASTG 4 cut(s) 40, 478, 559, 574
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 3 cut(s) 424, 505, 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 653
XspI CTAG 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.