Prupe.4G013100_v2.0.a1

RPM1-interacting protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014682
Physical Location & Seq
Reverse (-)
616089 .. 616947
859 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G013100.1

Sequence Viewer

Length: 732 bp
ATGACTACGTTGCAGAAAAAAACCCATGTCCCGAAATTTGGGGACTGGGACAGTGAAAATATATCCTACACTGCTTGTTTTGAGAACGCTCGTAAAGATGGGGTGAAGAGGAACCCAAATGATCCTGAAGAGAACCCAGAGGCCTTCAAGGCCATGCTTGATTTCCAAGCGGCACAGCCTCCACACAATGGCATGAGTTCCAGCAGCACATCAAGAACCTTGACAAACCCTAAAGCAGACCAAACTGGGGGAGACGCAGGCCATGGGGCGCATCGACGAAAGACTTCTGTAGGGAGCCGCAAGAGTAACATGTCTGAGTCTGGCAGTGAAAGGAGCAACTCAGATTATTCACTGTTGCAGCAGCAACAGTCAAGACACAGACGTGAGAGATCTGATAGAAGAAAGAGTAGTTTGGCTTCGTCTGGAACTGGAAGCGATCATAGCAGCACGTCCCATTCTCAGAGCAAGCCTAAGAGTACTACCAGTAGCCATCACTCTCACTCTGATCTTCTTAGTCACGACACTCATACTAGAGTAGCGTCAATACCCAAGTTTGGGGATTGGGATGAAAAAGACCCCCAATCGGGAGAAGGTTTTACTTACATATTCGAGCAAGTGAAAGAAGAAAAAAAAACTGCATCACAATTTCCCAATATGCCCCAACAACAACCAGTCAATCATTTAAATAGTGCAAATAAACGTGGAAAATCTTATTCCAATTCCAAATCAAAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

244

Amino Acids

27.09

Weight (kDa)

9.52

Isoelectric Point (pI)

63.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 188
AciI CCGC 2 cut(s) 170, 298
AclWI GGATC 1 cut(s) 116
AcsI RAATTY 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 7 cut(s) 38, 188, 247, 554, 555, 583, 584
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 1 cut(s) 148
AjiI CACGTC 2 cut(s) 383, 450
AleI CACNNNNGTG 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 116
AoxI GGCC 3 cut(s) 141, 150, 259
ApeKI GCWGC 4 cut(s) 204, 358, 361, 444
ApoI RAATTY 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 115
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbvI GCAGC 4 cut(s) 216, 370, 373, 456
BccI CCATC 2 cut(s) 92, 498
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 531
BfmI CTRYAG 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 389
BisI GCNGC 6 cut(s) 171, 205, 298, 359, 362, 445
BlsI GCNGC 6 cut(s) 172, 206, 299, 360, 363, 446
BmcAI AGTACT 1 cut(s) 478
BmgBI CACGTC 2 cut(s) 383, 450
BmiI GGNNCC 2 cut(s) 113, 296
BmrI ACTGGG 2 cut(s) 55, 255
BmsI GCATC 2 cut(s) 280, 647
BmuI ACTGGG 2 cut(s) 55, 255
BsaJI CCNNGG 1 cut(s) 262
Bsc4I CCNNNNNNNGG 7 cut(s) 38, 188, 247, 554, 555, 583, 584
Bse1I ACTGG 5 cut(s) 50, 250, 433, 483, 671
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 1 cut(s) 571
BseLI CCNNNNNNNGG 7 cut(s) 38, 188, 247, 554, 555, 583, 584
BseMII CTCAG 3 cut(s) 306, 354, 473
BseNI ACTGG 5 cut(s) 50, 250, 433, 483, 671
BseXI GCAGC 4 cut(s) 216, 370, 373, 456
BshFI GGCC 3 cut(s) 143, 152, 261
BslFI GGGAC 4 cut(s) 14, 56, 62, 436
BslI CCNNNNNNNGG 7 cut(s) 38, 188, 247, 554, 555, 583, 584
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 4 cut(s) 14, 56, 62, 436
BsnI GGCC 3 cut(s) 143, 152, 261
Bsp143I GATC 4 cut(s) 121, 389, 436, 505
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 2 cut(s) 170, 298
BspANI GGCC 3 cut(s) 143, 152, 261
BspCNI CTCAG 3 cut(s) 307, 353, 472
BspLI GGNNCC 2 cut(s) 113, 296
BspPI GGATC 1 cut(s) 116
BsrI ACTGG 5 cut(s) 50, 250, 433, 483, 671
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 4 cut(s) 121, 389, 436, 505
BssT1I CCWWGG 1 cut(s) 262
Bst4CI ACNGT 3 cut(s) 53, 354, 369
Bst6I CTCTTC 2 cut(s) 101, 123
BstC8I GCNNGC 2 cut(s) 259, 467
BstDEI CTNAG 5 cut(s) 315, 340, 459, 471, 512
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 1 cut(s) 571
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 4 cut(s) 124, 392, 439, 508
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 4 cut(s) 121, 389, 436, 505
BstMWI GCNNNNNNNGC 2 cut(s) 149, 441
BstNSI RCATGY 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 4 cut(s) 216, 370, 373, 456
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 3 cut(s) 143, 152, 261
BtgI CCRYGG 1 cut(s) 262
BtrI CACGTC 2 cut(s) 383, 450
BtsCI GGATG 1 cut(s) 571
BtsI GCAGTG 2 cut(s) 69, 331
BtsIMutI CAGTG 4 cut(s) 58, 69, 331, 350
Cac8I GCNNGC 2 cut(s) 259, 467
CfoI GCGC 1 cut(s) 271
CseI GACGC 2 cut(s) 263, 528
Csp6I GTAC 1 cut(s) 477
CviAII CATG 5 cut(s) 26, 154, 193, 263, 310
CviJI RGCY 8 cut(s) 143, 152, 178, 261, 297, 416, 469, 489
CviKI_1 RGCY 8 cut(s) 143, 152, 178, 261, 297, 416, 469, 489
CviQI GTAC 1 cut(s) 477
DdeI CTNAG 5 cut(s) 315, 340, 459, 471, 512
DpnI GATC 4 cut(s) 123, 391, 438, 507
DpnII GATC 4 cut(s) 121, 389, 436, 505
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 2 cut(s) 101, 123
EarI CTCTTC 2 cut(s) 101, 123
Eco130I CCWWGG 1 cut(s) 262
Eco147I AGGCCT 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 5 cut(s) 29, 157, 196, 266, 313
FaqI GGGAC 4 cut(s) 14, 56, 62, 436
FatI CATG 5 cut(s) 25, 153, 192, 262, 309
Fnu4HI GCNGC 6 cut(s) 171, 205, 298, 359, 362, 445
FokI GGATG 1 cut(s) 578
Fsp4HI GCNGC 6 cut(s) 171, 205, 298, 359, 362, 445
FspBI CTAG 1 cut(s) 531
GlaI GCGC 1 cut(s) 270
GluI GCNGC 6 cut(s) 171, 205, 298, 359, 362, 445
HaeIII GGCC 3 cut(s) 143, 152, 261
HgaI GACGC 2 cut(s) 263, 528
HhaI GCGC 1 cut(s) 271
Hin1II CATG 5 cut(s) 29, 157, 196, 266, 313
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 1 cut(s) 317
HphI GGTGA 1 cut(s) 115
Hpy188I TCNGA 5 cut(s) 316, 343, 394, 462, 505
Hpy188III TCNNGA 7 cut(s) 31, 125, 213, 372, 423, 518, 585
Hpy99I CGWCG 1 cut(s) 279
HpyAV CCTTC 2 cut(s) 154, 584
HpyCH4III ACNGT 3 cut(s) 53, 354, 369
HpyCH4IV ACGT 4 cut(s) 8, 382, 449, 700
HpyCH4V TGCA 4 cut(s) 13, 358, 638, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 441
HpyF3I CTNAG 5 cut(s) 315, 340, 459, 471, 512
HpySE526I ACGT 4 cut(s) 8, 382, 449, 700
Hsp92II CATG 5 cut(s) 29, 157, 196, 266, 313
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 4 cut(s) 121, 389, 436, 505
LmnI GCTCC 2 cut(s) 294, 333
Lsp1109I GCAGC 4 cut(s) 216, 370, 373, 456
LweI GCATC 2 cut(s) 280, 647
MaeI CTAG 1 cut(s) 531
MaeII ACGT 4 cut(s) 8, 382, 449, 700
MaeIII GTNAC 2 cut(s) 305, 515
MalI GATC 4 cut(s) 123, 391, 438, 507
MboI GATC 4 cut(s) 121, 389, 436, 505
MboII GAAGA 5 cut(s) 118, 140, 411, 500, 635
MflI RGATCY 1 cut(s) 389
MluCI AATT 3 cut(s) 35, 644, 718
MlyI GAGTC 1 cut(s) 326
MnlI CCTC 3 cut(s) 102, 133, 189
MroXI GAANNNNTTC 1 cut(s) 283
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 1 cut(s) 381
MwoI GCNNNNNNNGC 2 cut(s) 149, 441
NcoI CCATGG 1 cut(s) 262
NdeII GATC 4 cut(s) 121, 389, 436, 505
NlaIII CATG 5 cut(s) 29, 157, 196, 266, 313
NlaIV GGNNCC 2 cut(s) 113, 296
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 1 cut(s) 313
OliI CACNNNNGTG 1 cut(s) 381
PceI AGGCCT 1 cut(s) 143
PciI ACATGT 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 283
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 6 cut(s) 172, 206, 299, 360, 363, 446
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PscI ACATGT 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 113, 296
PsuI RGATCY 1 cut(s) 389
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
RseI CAYNNNNRTG 1 cut(s) 381
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 6 cut(s) 171, 205, 298, 359, 362, 445
Sau3AI GATC 4 cut(s) 121, 389, 436, 505
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 1 cut(s) 326
SetI ASST 6 cut(s) 11, 221, 385, 452, 595, 703
SfaNI GCATC 2 cut(s) 280, 647
SfcI CTRYAG 1 cut(s) 288
SfiI GGCCNNNNNGGCC 1 cut(s) 149
SmiI ATTTAAAT 1 cut(s) 684
SmiMI CAYNNNNRTG 1 cut(s) 381
Sse9I AATT 3 cut(s) 35, 644, 718
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 2 cut(s) 170, 298
SspMI CTAG 1 cut(s) 531
StuI AGGCCT 1 cut(s) 143
StyI CCWWGG 1 cut(s) 262
SwaI ATTTAAAT 1 cut(s) 684
TaaI ACNGT 3 cut(s) 53, 354, 369
TaiI ACGT 4 cut(s) 11, 385, 452, 703
TaqI TCGA 2 cut(s) 274, 609
TasI AATT 3 cut(s) 35, 644, 718
TatI WGTACW 1 cut(s) 476
TauI GCSGC 2 cut(s) 173, 300
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 4 cut(s) 58, 76, 331, 357
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 4 cut(s) 204, 358, 361, 444
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 582
TspRI CASTG 4 cut(s) 58, 76, 331, 357
Van91I CCANNNNNTGG 1 cut(s) 188
XapI RAATTY 1 cut(s) 35
XceI RCATGY 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 283
XspI CTAG 1 cut(s) 531
ZrmI AGTACT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.