pycom10g27870

RPM1-interacting protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014682
Physical Location & Seq
Forward (+)
28723837 .. 28724658
822 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27870.1

Sequence Viewer

Length: 603 bp
ATGTCATCTCGATTCATGGAAGTCCTCAAAAAACCCCATGTCCCAAAGTTTGGGGACTGGGACAGTGACAACATATCATACACTGCATGTTTTGAAAGTGCTCGTAAAAATGGAGTGAAGGGAAATCCAAATGATCCCGAAGACAACCCAGAGGCCTTCAAGAGCATGCTCAAGTCCTCCGCCGCACAACCTGCTTACATATCCACGAGTTCGAGCAGCAAACCACGAACCTCAAGCGAAAAAGTACGCCAAACTGAAGGAGACAATAGCCACCGTGCGCATGAGCATAGGGTTTCTGGAGGGAGCCGCAAGAGTTACACATCGGAGTCTGGCAGCGAAAGGAGCAACTCTGATTATTCGCTCTTGCAGCAACAACCGAGACACCACCGTGAGAGATCTGATAGAAAAAGTAGCTTGGCTTCATCAGGAAGTGATCGTAGTCATAGCGCTTCCAATTCCCAGAGCAAGCCTAAAAGTACTGGCAGTAGCACTCATCAGTCCAGTAATCATATTCAACACGATACTCTCGTAAATTGTGATCGTCCCACTTACATAGAATTCTTTGCTAAAGTATGCAAGATTTGTAATGTGACAGCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

201

Amino Acids

22.2

Weight (kDa)

9.28

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 12 - 37 7.2e-09 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 199
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 3 cut(s) 180, 183, 307
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 2 cut(s) 246, 478
AfeI AGCGCT 1 cut(s) 448
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 3 cut(s) 95, 160, 515
AleI CACNNNNGTG 1 cut(s) 387
AluBI AGCT 1 cut(s) 414
AluI AGCT 1 cut(s) 414
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 2 cut(s) 255, 373
AlwI GGATC 1 cut(s) 128
Aor51HI AGCGCT 1 cut(s) 448
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 3 cut(s) 216, 333, 367
ApoI RAATTY 1 cut(s) 557
AspLEI GCGC 2 cut(s) 280, 449
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BarI GAAGNNNNNNTAC 2 cut(s) 403, 435
BauI CACGAG 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 228, 345, 379
BcoDI GTCTC 2 cut(s) 255, 373
BfaI CTAG 1 cut(s) 601
BfoI RGCGCY 1 cut(s) 450
BfuAI ACCTGC 1 cut(s) 199
BglII AGATCT 1 cut(s) 395
BisI GCNGC 5 cut(s) 183, 217, 307, 334, 368
BlsI GCNGC 5 cut(s) 184, 218, 308, 335, 369
BmcAI AGTACT 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 305
BmrI ACTGGG 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 67
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 318
BpuEI CTTGAG 2 cut(s) 155, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 62, 484, 501
BseLI CCNNNNNNNGG 1 cut(s) 50
BseNI ACTGG 3 cut(s) 62, 484, 501
BseXI GCAGC 3 cut(s) 228, 345, 379
BshFI GGCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 103
BslFI GGGAC 4 cut(s) 26, 68, 74, 528
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 2 cut(s) 255, 373
BsmFI GGGAC 4 cut(s) 26, 68, 74, 528
BsnI GGCC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 4 cut(s) 133, 395, 433, 538
BspACI CCGC 3 cut(s) 180, 183, 307
BspANI GGCC 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 305
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 1 cut(s) 128
BsrI ACTGG 3 cut(s) 62, 484, 501
BssMI GATC 4 cut(s) 133, 395, 433, 538
BssSI CACGAG 1 cut(s) 205
Bst2BI CACGAG 1 cut(s) 205
Bst4CI ACNGT 3 cut(s) 65, 275, 389
BstAPI GCANNNNNTGC 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 167, 467
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 280, 449
BstKTI GATC 4 cut(s) 136, 398, 436, 541
BstMAI GTCTC 2 cut(s) 255, 373
BstMBI GATC 4 cut(s) 133, 395, 433, 538
BstMWI GCNNNNNNNGC 3 cut(s) 191, 342, 367
BstNSI RCATGY 2 cut(s) 90, 169
BstV1I GCAGC 3 cut(s) 228, 345, 379
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
BsuRI GGCC 1 cut(s) 155
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 2 cut(s) 70, 81
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 167, 467
CfoI GCGC 2 cut(s) 280, 449
Csp6I GTAC 2 cut(s) 245, 477
CviAII CATG 5 cut(s) 16, 38, 87, 166, 281
CviJI RGCY 6 cut(s) 155, 270, 306, 414, 419, 469
CviKI_1 RGCY 6 cut(s) 155, 270, 306, 414, 419, 469
CviQI GTAC 2 cut(s) 245, 477
DpnI GATC 4 cut(s) 135, 397, 435, 540
DpnII GATC 4 cut(s) 133, 395, 433, 538
EciI GGCGGA 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 155
Eco47III AGCGCT 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 276
EcoRI GAATTC 1 cut(s) 557
FaeI CATG 5 cut(s) 19, 41, 90, 169, 284
FaqI GGGAC 4 cut(s) 26, 68, 74, 528
FatI CATG 5 cut(s) 15, 37, 86, 165, 280
Fnu4HI GCNGC 5 cut(s) 183, 217, 307, 334, 368
Fsp4HI GCNGC 5 cut(s) 183, 217, 307, 334, 368
FspAI RTGCGCAY 1 cut(s) 279
FspBI CTAG 1 cut(s) 601
FspI TGCGCA 1 cut(s) 279
GlaI GCGC 2 cut(s) 279, 448
GluI GCNGC 5 cut(s) 183, 217, 307, 334, 368
GsuI CTGGAG 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 1 cut(s) 155
HhaI GCGC 2 cut(s) 280, 449
Hin1II CATG 5 cut(s) 19, 41, 90, 169, 284
Hin6I GCGC 2 cut(s) 278, 447
HinP1I GCGC 2 cut(s) 278, 447
HinfI GANTC 2 cut(s) 12, 326
Hpy188I TCNGA 3 cut(s) 325, 352, 400
Hpy188III TCNNGA 5 cut(s) 9, 137, 160, 297, 426
HpyAV CCTTC 3 cut(s) 112, 166, 251
HpyCH4III ACNGT 3 cut(s) 65, 275, 389
HpyCH4V TGCA 3 cut(s) 86, 367, 576
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 342, 367
Hsp92II CATG 5 cut(s) 19, 41, 90, 169, 284
HspAI GCGC 2 cut(s) 278, 447
Kzo9I GATC 4 cut(s) 133, 395, 433, 538
LmnI GCTCC 2 cut(s) 303, 342
LpnPI CCDG 9 cut(s) 43, 162, 204, 282, 315, 411, 465, 473, 514
Lsp1109I GCAGC 3 cut(s) 228, 345, 379
MaeI CTAG 1 cut(s) 601
MaeIII GTNAC 3 cut(s) 65, 314, 589
MalI GATC 4 cut(s) 135, 397, 435, 540
MboI GATC 4 cut(s) 133, 395, 433, 538
MboII GAAGA 1 cut(s) 152
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 3 cut(s) 454, 532, 557
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 5 cut(s) 35, 145, 187, 241, 293
MslI CAYNNNNRTG 1 cut(s) 387
MwoI GCNNNNNNNGC 3 cut(s) 191, 342, 367
NdeII GATC 4 cut(s) 133, 395, 433, 538
NlaIII CATG 5 cut(s) 19, 41, 90, 169, 284
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 2 cut(s) 65, 589
NsbI TGCGCA 1 cut(s) 279
NspI RCATGY 2 cut(s) 90, 169
OliI CACNNNNGTG 1 cut(s) 387
PaeI GCATGC 1 cut(s) 169
PceI AGGCCT 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 525
PfeI GAWTC 1 cut(s) 12
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 5 cut(s) 184, 218, 308, 335, 369
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 395
RsaI GTAC 2 cut(s) 246, 478
RsaNI GTAC 2 cut(s) 245, 477
RseI CAYNNNNRTG 1 cut(s) 387
SatI GCNGC 5 cut(s) 183, 217, 307, 334, 368
Sau3AI GATC 4 cut(s) 133, 395, 433, 538
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 1 cut(s) 335
SduI GDGCHC 1 cut(s) 103
SetI ASST 3 cut(s) 193, 233, 416
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 170, 232
SmoI CTYRAG 2 cut(s) 170, 232
SphI GCATGC 1 cut(s) 169
Sse9I AATT 3 cut(s) 454, 532, 557
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 3 cut(s) 180, 183, 307
SspMI CTAG 1 cut(s) 601
StuI AGGCCT 1 cut(s) 155
TaaI ACNGT 3 cut(s) 65, 275, 389
TaqI TCGA 2 cut(s) 10, 212
TasI AATT 3 cut(s) 454, 532, 557
TatI WGTACW 1 cut(s) 476
TauI GCSGC 2 cut(s) 185, 309
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 2 cut(s) 70, 88
TseFI GTSAC 2 cut(s) 65, 589
TseI GCWGC 3 cut(s) 216, 333, 367
Tsp45I GTSAC 2 cut(s) 65, 589
TspDTI ATGAA 2 cut(s) 4, 411
TspRI CASTG 2 cut(s) 70, 88
Van91I CCANNNNNTGG 1 cut(s) 50
XapI RAATTY 1 cut(s) 557
XceI RCATGY 2 cut(s) 90, 169
XspI CTAG 1 cut(s) 601
ZrmI AGTACT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.