Prupe.4G256800_v2.0.a1

THUMP domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012337
Physical Location & Seq
Forward (+)
18083203 .. 18089590
6388 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G256800.1

Sequence Viewer

Length: 1389 bp
ATGGCGACAGATAGCAAATCCAAGTCCACTTCCGCAGCCAATAAGTCCAAGAAGGGGAAGCAACGGTATCTCCCTCACAACAAAGCGGTGAAGAAGAAAGGGTCGTACCCATTACACCCTGGAGTGCAAGGCTTCTTTATTACTTGTGATGGTGGCAGGGAACGCCAAGCCTCTAATGAGGCCATTAACGTTATTGACTCTTTTTATGAAGAGCTAGTCCATGGGAAAGGTTCAGGATTAAAGCTTTCTGAGGAACACAAACCTTCAAATAAGAAGATTAAATTCTCATATTCTGATTCTTCTAGTAGTGATGATGATGATGATCACGAAAACAAACAGGCGGGAGAGGACAAGGCAGAAGAAGAACAAGAAAATGGGGTGGATGAAGAGAATAAATCTGATACTCGTGGAGATGATGGTAGTCATGATAATCCAACAACTGAGAAATCAGATCACCAGAAGAAAGATGATGCTAGTCAAGATAATGCAACAACTGAAAAATCAGATCACCAGAAGAAAGATGATGGTAGTCATGATAATGCGATAACTGAGAAATCAGATCACCAGAAGAAAGATGATGCTAGTCAAGATAATGCAACAACTGAAAAATCAGATCACCAGAAGAAAGATGATGGTAGTCAAGATAATGCAGCAACTGAGAAATCAGATCACCATAAGAAAGATGATGGATGTGATGGAAATAAAACTGAGGAAAAGATTAAAGACACTAAAGAGGACAATGTTAGTAAGGAAATTTCAGCAAATGAAGCTGAGGAGCCACCAGCAAAGAAACAATGTCTAGGAACAAATTCATCAAAATGTACGATCCCATATGAAGTGGAAGAGAAGTCCATTGATAAGCTTATTGAAGCTGAGCTTCAAGAACTGGGAGATAAGAACAAGAGGCGCTTTATCAGCCTTGATTCTGGTTGCAATGGTGTTGTCTTTGTTCAAATGCGGAAGAGAGATGGAGATCCTAGCCCAAAGGATATAGTGCAGCATATGATGGCATCTGCTGCTGCAACCAGGAGACACATGTCCAGGTTTATCTTAAGAGTATTGCCTATTGAAGTAGCGTGCTATTCTTCTGAAGAGGATATTTCAAGAGCAATCAAACCACTTCTTGCACAGCACTTTCCAGTGGAAACTCAGAATCCTCAGAAGTTTGCAGTCCTGTATGAAGCCCGTGCAAATACTGGTATTGACAGGATGAAAATCATAAATGCAGTGGCAAAATCCGTGCCTGGGCCTCACAAAGTTGACCTTAAAAATCCTGATAAGACCATTGTTGTTGAAATTGTGAAGACTATTTGCTTGATCGGTGTTGTTGACAAGTACAAGGAGTTGGCCAAATACAACTTGAGGCAGCTCACATCACCAAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
THUMP

Protein Analysis

463

Amino Acids

51.36

Weight (kDa)

5.91

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12410
fragaria_vesca FvH4_3g28330 FvH4_3g28330
malus_domestica MD03G1170300.v1.1 MD11G1187800.v1.1
prunus_persica Prupe.4G256800_v2.0.a1
pyrus_communis pycom03g12680 pycom11g16450
rosa_chinensis RchiOBHm_Chr5g0051211
rosa_laevigata RLG00000034763
rosa_multiflora Rmu_sc0002385.1_g000021 Rmu_sc0042180.1_g000001
rosa_roxburghii Rroxscaffold_1G00029150
rosa_rugosa Rorug05G0266700
rosa_samantha Rh5AG339000 Rh5BG348900 Rh5CG375900 Rh5DG361800
rosa_wichuraiana Rw5G032030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 86, 341, 958
AclI AACGTT 1 cut(s) 189
AclWI GGATC 2 cut(s) 820, 968
AcoI YGGCCR 1 cut(s) 1347
AcsI RAATTY 3 cut(s) 281, 753, 808
AcuI CTGAAG 1 cut(s) 1110
AfaI GTAC 3 cut(s) 107, 823, 1337
AfiI CCNNNNNNNGG 2 cut(s) 54, 1245
AflII CTTAAG 1 cut(s) 1051
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 7 cut(s) 267, 869, 881, 953, 1070, 1104, 1295
AjnI CCWGG 4 cut(s) 118, 1025, 1040, 1243
AluBI AGCT 7 cut(s) 214, 244, 770, 862, 872, 877, 1369
AluI AGCT 7 cut(s) 214, 244, 770, 862, 872, 877, 1369
Alw26I GTCTC 1 cut(s) 1024
AlwI GGATC 2 cut(s) 820, 968
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 3 cut(s) 180, 1247, 1347
ApeKI GCWGC 6 cut(s) 35, 650, 997, 1016, 1019, 1366
ApoI RAATTY 3 cut(s) 281, 753, 808
Asp700I GAANNNNTTC 1 cut(s) 808
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 1 cut(s) 1247
AsuHPI GGTGA 7 cut(s) 100, 446, 500, 554, 608, 662, 1368
BalI TGGCCA 1 cut(s) 1349
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 1 cut(s) 1310
BbvCI CCTCAGC 1 cut(s) 771
BbvI GCAGC 6 cut(s) 47, 662, 1003, 1006, 1009, 1378
BccI CCATC 8 cut(s) 143, 410, 518, 626, 680, 689, 962, 1000
BciT130I CCWGG 4 cut(s) 120, 1027, 1042, 1245
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 1024
BfaI CTAG 6 cut(s) 215, 303, 474, 582, 800, 978
BfoI RGCGCY 1 cut(s) 910
BfrI CTTAAG 1 cut(s) 1051
BisI GCNGC 6 cut(s) 36, 651, 998, 1017, 1020, 1367
BlpI GCTNAGC 1 cut(s) 873
BlsI GCNGC 6 cut(s) 37, 652, 999, 1018, 1021, 1368
Bme1390I CCNGG 4 cut(s) 120, 1027, 1042, 1245
BmgT120I GGNCC 1 cut(s) 1247
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 4 cut(s) 120, 1027, 1042, 1245
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 3 cut(s) 460, 568, 1019
BmuI ACTGGG 1 cut(s) 896
BoxI GACNNNNGTC 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 1310
BpmI CTGGAG 1 cut(s) 141
Bpu10I CCTNAGC 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 1381
BsaBI GATNNNNATC 3 cut(s) 321, 972, 1214
BsaJI CCNNGG 3 cut(s) 118, 220, 1244
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 1245
Bse1I ACTGG 3 cut(s) 891, 1139, 1201
Bse3DI GCAATG 1 cut(s) 940
Bse8I GATNNNNATC 3 cut(s) 321, 972, 1214
BseBI CCWGG 4 cut(s) 120, 1027, 1042, 1245
BseDI CCNNGG 3 cut(s) 118, 220, 1244
BseGI GGATG 3 cut(s) 388, 695, 1215
BseJI GATNNNNATC 3 cut(s) 321, 972, 1214
BseLI CCNNNNNNNGG 2 cut(s) 54, 1245
BseMI GCAATG 1 cut(s) 940
BseMII CTCAG 9 cut(s) 240, 432, 540, 648, 699, 762, 864, 1163, 1172
BseNI ACTGG 3 cut(s) 891, 1139, 1201
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 6 cut(s) 47, 662, 1003, 1006, 1009, 1378
BsgI GTGCAG 1 cut(s) 1016
BshFI GGCC 3 cut(s) 182, 1249, 1349
BslI CCNNNNNNNGG 2 cut(s) 54, 1245
BsmAI GTCTC 1 cut(s) 1024
BsnI GGCC 3 cut(s) 182, 1249, 1349
Bsp143I GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
Bsp1720I GCTNAGC 1 cut(s) 873
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 4 cut(s) 33, 86, 341, 958
BspANI GGCC 3 cut(s) 182, 1249, 1349
BspCNI CTCAG 9 cut(s) 241, 433, 541, 649, 700, 763, 865, 1162, 1171
BspHI TCATGA 2 cut(s) 424, 532
BspLI GGNNCC 1 cut(s) 777
BspPI GGATC 2 cut(s) 820, 968
BspQI GCTCTTC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 1051
BsrDI GCAATG 1 cut(s) 940
BsrI ACTGG 3 cut(s) 891, 1139, 1201
BssECI CCNNGG 3 cut(s) 118, 220, 1244
BssMI GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 4 cut(s) 120, 1027, 1042, 1245
Bst4CI ACNGT 1 cut(s) 66
Bst6I CTCTTC 5 cut(s) 204, 381, 837, 956, 1086
BstAFI CTTAAG 1 cut(s) 1051
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstC8I GCNNGC 1 cut(s) 1078
BstDEI CTNAG 9 cut(s) 249, 441, 549, 657, 708, 771, 873, 1149, 1158
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 3 cut(s) 388, 695, 1215
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 9 cut(s) 325, 454, 508, 562, 616, 670, 828, 976, 1320
BstMAI GTCTC 1 cut(s) 1024
BstMBI GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
BstMWI GCNNNNNNNGC 4 cut(s) 162, 767, 915, 1016
BstNI CCWGG 4 cut(s) 120, 1027, 1042, 1245
BstNSI RCATGY 1 cut(s) 1039
BstPAI GACNNNNGTC 1 cut(s) 1036
BstSCI CCNGG 4 cut(s) 118, 1025, 1040, 1243
BstV1I GCAGC 6 cut(s) 47, 662, 1003, 1006, 1009, 1378
BstV2I GAAGAC 1 cut(s) 1310
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
BsuRI GGCC 3 cut(s) 182, 1249, 1349
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 388, 695, 1215
BtsI GCAGTG 1 cut(s) 1233
BtsIMutI CAGTG 2 cut(s) 1146, 1233
Cac8I GCNNGC 1 cut(s) 1078
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 2 cut(s) 424, 532
CfoI GCGC 1 cut(s) 909
Cfr13I GGNCC 1 cut(s) 1247
Csp6I GTAC 3 cut(s) 106, 822, 1336
CviAII CATG 4 cut(s) 221, 425, 533, 1036
CviQI GTAC 3 cut(s) 106, 822, 1336
DdeI CTNAG 9 cut(s) 249, 441, 549, 657, 708, 771, 873, 1149, 1158
DpnI GATC 9 cut(s) 324, 453, 507, 561, 615, 669, 827, 975, 1319
DpnII GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
EaeI YGGCCR 1 cut(s) 1347
Eam1104I CTCTTC 5 cut(s) 204, 381, 837, 956, 1086
EarI CTCTTC 5 cut(s) 204, 381, 837, 956, 1086
Eco130I CCWWGG 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 1110
EcoRII CCWGG 4 cut(s) 118, 1025, 1040, 1243
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 4 cut(s) 224, 428, 536, 1039
FalI AAGNNNNNCTT 6 cut(s) 861, 893, 893, 925, 1248, 1280
FatI CATG 4 cut(s) 220, 424, 532, 1035
FauI CCCGC 1 cut(s) 334
FauNDI CATATG 2 cut(s) 832, 1002
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 6 cut(s) 36, 651, 998, 1017, 1020, 1367
FokI GGATG 3 cut(s) 395, 702, 1222
Fsp4HI GCNGC 6 cut(s) 36, 651, 998, 1017, 1020, 1367
FspBI CTAG 6 cut(s) 215, 303, 474, 582, 800, 978
GlaI GCGC 1 cut(s) 908
GluI GCNGC 6 cut(s) 36, 651, 998, 1017, 1020, 1367
GsuI CTGGAG 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 910
HaeIII GGCC 3 cut(s) 182, 1249, 1349
HhaI GCGC 1 cut(s) 909
Hin1II CATG 4 cut(s) 224, 428, 536, 1039
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HincII GTYRAC 2 cut(s) 1261, 1330
HindII GTYRAC 2 cut(s) 1261, 1330
HindIII AAGCTT 2 cut(s) 242, 860
HinfI GANTC 4 cut(s) 197, 296, 923, 1153
HphI GGTGA 7 cut(s) 100, 446, 500, 554, 608, 662, 1368
Hpy166II GTNNAC 3 cut(s) 27, 1261, 1330
Hpy8I GTNNAC 3 cut(s) 27, 1261, 1330
HpyAV CCTTC 2 cut(s) 46, 273
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4IV ACGT 1 cut(s) 189
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 767, 915, 1016
HpyF3I CTNAG 9 cut(s) 249, 441, 549, 657, 708, 771, 873, 1149, 1158
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 4 cut(s) 224, 428, 536, 1039
HspAI GCGC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 6 cut(s) 47, 662, 1003, 1006, 1009, 1378
LweI GCATC 3 cut(s) 460, 568, 1019
MaeI CTAG 6 cut(s) 215, 303, 474, 582, 800, 978
MaeII ACGT 1 cut(s) 189
MalI GATC 9 cut(s) 324, 453, 507, 561, 615, 669, 827, 975, 1319
MboI GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
MflI RGATCY 1 cut(s) 973
MlsI TGGCCA 1 cut(s) 1349
MluCI AATT 4 cut(s) 281, 753, 808, 1296
MluNI TGGCCA 1 cut(s) 1349
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 458
Mox20I TGGCCA 1 cut(s) 1349
MroXI GAANNNNTTC 1 cut(s) 808
MscI TGGCCA 1 cut(s) 1349
MseI TTAA 6 cut(s) 186, 239, 279, 720, 1052, 1266
MslI CAYNNNNRTG 2 cut(s) 537, 817
Msp20I TGGCCA 1 cut(s) 1349
MspCI CTTAAG 1 cut(s) 1051
MspR9I CCNGG 4 cut(s) 120, 1027, 1042, 1245
MvaI CCWGG 4 cut(s) 120, 1027, 1042, 1245
MwoI GCNNNNNNNGC 4 cut(s) 162, 767, 915, 1016
NcoI CCATGG 1 cut(s) 220
NdeI CATATG 2 cut(s) 832, 1002
NdeII GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
NlaIII CATG 4 cut(s) 224, 428, 536, 1039
NlaIV GGNNCC 1 cut(s) 777
NspI RCATGY 1 cut(s) 1039
PagI TCATGA 2 cut(s) 424, 532
PciI ACATGT 1 cut(s) 1035
PciSI GCTCTTC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 808
PfeI GAWTC 3 cut(s) 296, 923, 1153
PkrI GCNGC 6 cut(s) 37, 652, 999, 1018, 1021, 1368
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PscI ACATGT 1 cut(s) 1035
PshAI GACNNNNGTC 1 cut(s) 1036
Psp1406I AACGTT 1 cut(s) 189
Psp6I CCWGG 4 cut(s) 118, 1025, 1040, 1243
PspGI CCWGG 4 cut(s) 118, 1025, 1040, 1243
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 1 cut(s) 1247
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 973
RsaI GTAC 3 cut(s) 107, 823, 1337
RsaNI GTAC 3 cut(s) 106, 822, 1336
RseI CAYNNNNRTG 2 cut(s) 537, 817
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 6 cut(s) 186, 239, 279, 720, 1052, 1266
SatI GCNGC 6 cut(s) 36, 651, 998, 1017, 1020, 1367
Sau3AI GATC 9 cut(s) 322, 451, 505, 559, 613, 667, 825, 973, 1317
Sau96I GGNCC 1 cut(s) 1247
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 4 cut(s) 120, 1027, 1042, 1245
SfaNI GCATC 3 cut(s) 460, 568, 1019
SmiMI CAYNNNNRTG 2 cut(s) 537, 817
SmlI CTYRAG 2 cut(s) 1051, 1360
SmoI CTYRAG 2 cut(s) 1051, 1360
Sse9I AATT 4 cut(s) 281, 753, 808, 1296
SsiI CCGC 4 cut(s) 33, 86, 341, 958
SspMI CTAG 6 cut(s) 215, 303, 474, 582, 800, 978
StyD4I CCNGG 4 cut(s) 118, 1025, 1040, 1243
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 66
TaiI ACGT 1 cut(s) 192
TasI AATT 4 cut(s) 281, 753, 808, 1296
TatI WGTACW 1 cut(s) 1335
TfiI GAWTC 3 cut(s) 296, 923, 1153
Tru1I TTAA 6 cut(s) 186, 239, 279, 720, 1052, 1266
Tru9I TTAA 6 cut(s) 186, 239, 279, 720, 1052, 1266
TscAI CASTG 2 cut(s) 1146, 1233
TseI GCWGC 6 cut(s) 35, 650, 997, 1016, 1019, 1366
TspDTI ATGAA 7 cut(s) 222, 399, 780, 801, 849, 1194, 1226
TspGWI ACGGA 1 cut(s) 1228
TspRI CASTG 2 cut(s) 1146, 1233
Vha464I CTTAAG 1 cut(s) 1051
XapI RAATTY 3 cut(s) 281, 753, 808
XceI RCATGY 1 cut(s) 1039
XmnI GAANNNNTTC 1 cut(s) 808
XspI CTAG 6 cut(s) 215, 303, 474, 582, 800, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.