Rw5G032030

THUMP domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012337
Physical Location & Seq
Reverse (-)
53692957 .. 53697940
4984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032030.1

Sequence Viewer

Length: 1164 bp
ATGGCGACTGAGAATAATAAATCCAAGTGGTCCTCAGCCCCCAAGTCCAAGAAGGGAAAGACTCGTTACCTTCCTCACAACAGGTCGGTGAAGAAGAAAGGGTCGTATCCTTTACACCCGGGCGTGGAAGGATTCTTTATCACTTGTGATGGCGGCAGGGAACGCCAGGCCTCTCATGAAGCTCTCAACGTTATTGACTCGTTTTTTGAAGAGCTTGTACAGGGGAAGGATTCAGGGATAAATCTTTCTGAGGCACCCAGTAAACCTTTAAATAAAAAAATCAAGTTCACATACTCTGATTCTTCTAGTAGTGATGATGATAACAAAGAGGAGGGAGAAACTGAGGAACCTGTTGAAGACAAGGAGGTGGTCAAGGAAGAAAATATATCTGATACTTGTAGAGATGATGATGGTAGTCATGATAATCAAATAAGCGAGACATCAGATCTCGAGAAGAAAGACAATGCTCTCTGTGAAGATAAACCAGAGGAAGATACTAAAGACGACAAAGAGGGTAGTATAAGTAAAGAGATTTCTGCAAAAGAAGCTGAAGAGCCACCAGCAAAGAAACAATGTCTAGGAACAGATTCATCAAAAAGGATCCCGGATAAAGTAGAAGAGAAGTCCATTGATAAACTAATTGACGATGAGCTCCAAGAACTTAAAGATAAGAACAAGAGGCGCTTTGTCAACCTTGAGTCAGGTTGTAATGGTGTCGTCTTTGTTCAAATGCGGAAGAGAGATGGAGATCCTGGCCCCAAAGATATTGTACAGCATATGATGACATCTGCTGCTGCAACAAGGAAACACATGTCGAGGTTTATCTTAAGGGTATTGCCTGTCGAAGTATCATGCTATGCTTCAGAAGAGGAGATTTCCAGAGCAATTAAACCTCTTCTTGCACAATATTTTCCCTTGGAAACTCAGAGTCCTCAGAAGTTTGCAGTACTTTACGGAGCACGGGCAAATACTGGTATTGATAGGATGAAAATCATAGATGCAGTGGCAAAATCCGTGCCTGAACCTCACAAAGTTGACCTTAGCAACCCTGATTTGAACATTGTAGTTGAAATTTGCAAGACTATTTGCTTGATTGGTGTTGTTGAAAATTATAAGCAGTTGGGCAAGTACAACCTGAGGCAGCTCACATCACCTAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
THUMP

Protein Analysis

387

Amino Acids

43.33

Weight (kDa)

5.54

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THUMP PF02926 286 - 368 1e-16 THUMP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12410
fragaria_vesca FvH4_3g28330 FvH4_3g28330
malus_domestica MD03G1170300.v1.1 MD11G1187800.v1.1
prunus_persica Prupe.4G256800_v2.0.a1
pyrus_communis pycom03g12680 pycom11g16450
rosa_chinensis RchiOBHm_Chr5g0051211
rosa_laevigata RLG00000034763
rosa_multiflora Rmu_sc0002385.1_g000021 Rmu_sc0042180.1_g000001
rosa_roxburghii Rroxscaffold_1G00029150
rosa_rugosa Rorug05G0266700
rosa_samantha Rh5AG339000 Rh5BG348900 Rh5CG375900 Rh5DG361800
rosa_wichuraiana Rw5G032030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1113
AccB1I GGYRCC 1 cut(s) 253
AciI CCGC 2 cut(s) 153, 733
AclI AACGTT 1 cut(s) 189
AclWI GGATC 3 cut(s) 595, 608, 743
AcsI RAATTY 1 cut(s) 1071
AcuI CTGAAG 2 cut(s) 570, 846
AfaI GTAC 4 cut(s) 219, 771, 948, 1130
AfiI CCNNNNNNNGG 1 cut(s) 124
AflII CTTAAG 1 cut(s) 826
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 6 cut(s) 209, 356, 728, 1057, 1070, 1106
AjnI CCWGG 2 cut(s) 165, 751
AluBI AGCT 5 cut(s) 182, 214, 548, 652, 1144
AluI AGCT 5 cut(s) 182, 214, 548, 652, 1144
Alw21I GWGCWC 2 cut(s) 654, 961
Alw26I GTCTC 1 cut(s) 431
AlwI GGATC 3 cut(s) 595, 608, 743
Ama87I CYCGRG 2 cut(s) 118, 449
AoxI GGCC 2 cut(s) 168, 754
ApeKI GCWGC 3 cut(s) 791, 794, 1141
ApoI RAATTY 1 cut(s) 1071
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 1 cut(s) 586
AspLEI GCGC 1 cut(s) 684
AspS9I GGNCC 2 cut(s) 30, 755
AsuC2I CCSGG 3 cut(s) 119, 120, 605
AsuHPI GGTGA 2 cut(s) 100, 1143
AvaI CYCGRG 2 cut(s) 118, 449
AvaII GGWCC 1 cut(s) 30
AxyI CCTNAGG 1 cut(s) 1136
BamHI GGATCC 1 cut(s) 600
BanI GGYRCC 1 cut(s) 253
BanII GRGCYC 1 cut(s) 654
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 2 cut(s) 654, 961
BbvCI CCTCAGC 1 cut(s) 34
BbvI GCAGC 3 cut(s) 778, 781, 1153
BccI CCATC 3 cut(s) 143, 404, 737
BciT130I CCWGG 2 cut(s) 167, 753
BciVI GTATCC 1 cut(s) 117
BcnI CCSGG 3 cut(s) 119, 120, 605
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 2 cut(s) 306, 578
BfoI RGCGCY 1 cut(s) 685
BfrI CTTAAG 1 cut(s) 826
BfuI GTATCC 1 cut(s) 117
BglII AGATCT 1 cut(s) 445
BisI GCNGC 4 cut(s) 154, 792, 795, 1142
BlsI GCNGC 4 cut(s) 155, 793, 796, 1143
BmcAI AGTACT 1 cut(s) 948
Bme1390I CCNGG 5 cut(s) 119, 120, 167, 605, 753
Bme18I GGWCC 1 cut(s) 30
BmeT110I CYCGRG 2 cut(s) 118, 449
BmgT120I GGNCC 2 cut(s) 30, 755
BmiI GGNNCC 4 cut(s) 255, 348, 602, 757
BmrFI CCNGG 5 cut(s) 119, 120, 167, 605, 753
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 988
BmuI ACTGGG 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 363
Bpu10I CCTNAGC 3 cut(s) 34, 1040, 1155
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 3 cut(s) 119, 120, 605
BsaBI GATNNNNATC 2 cut(s) 747, 989
BsaJI CCNNGG 2 cut(s) 118, 915
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 2 cut(s) 258, 976
Bse21I CCTNAGG 1 cut(s) 1136
Bse8I GATNNNNATC 2 cut(s) 747, 989
BseBI CCWGG 2 cut(s) 167, 753
BseDI CCNNGG 2 cut(s) 118, 915
BseGI GGATG 1 cut(s) 990
BseJI GATNNNNATC 2 cut(s) 747, 989
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 6 cut(s) 48, 240, 333, 938, 947, 1127
BseNI ACTGG 2 cut(s) 258, 976
BseRI GAGGAG 2 cut(s) 344, 884
BseXI GCAGC 3 cut(s) 778, 781, 1153
BshFI GGCC 2 cut(s) 170, 756
BshNI GGYRCC 1 cut(s) 253
BsiHKAI GWGCWC 2 cut(s) 654, 961
BsiHKCI CYCGRG 2 cut(s) 118, 449
BsiSI CCGG 2 cut(s) 119, 605
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 431
BsnI GGCC 2 cut(s) 170, 756
BsoBI CYCGRG 2 cut(s) 118, 449
Bsp1286I GDGCHC 2 cut(s) 654, 961
Bsp1407I TGTACA 2 cut(s) 217, 769
Bsp143I GATC 3 cut(s) 445, 600, 748
BspACI CCGC 2 cut(s) 153, 733
BspANI GGCC 2 cut(s) 170, 756
BspCNI CTCAG 6 cut(s) 47, 241, 334, 937, 946, 1128
BspHI TCATGA 2 cut(s) 175, 418
BspLI GGNNCC 4 cut(s) 255, 348, 602, 757
BspPI GGATC 3 cut(s) 595, 608, 743
BspQI GCTCTTC 2 cut(s) 204, 546
BspT107I GGYRCC 1 cut(s) 253
BspTI CTTAAG 1 cut(s) 826
BsrGI TGTACA 2 cut(s) 217, 769
BsrI ACTGG 2 cut(s) 258, 976
BssECI CCNNGG 2 cut(s) 118, 915
BssMI GATC 3 cut(s) 445, 600, 748
BssT1I CCWWGG 1 cut(s) 915
Bst2UI CCWGG 2 cut(s) 167, 753
Bst6I CTCTTC 6 cut(s) 204, 546, 612, 731, 861, 900
BstAFI CTTAAG 1 cut(s) 826
BstAUI TGTACA 2 cut(s) 217, 769
BstDEI CTNAG 9 cut(s) 9, 34, 249, 342, 924, 933, 1040, 1136, 1155
BstF5I GGATG 1 cut(s) 990
BstH2I RGCGCY 1 cut(s) 685
BstHHI GCGC 1 cut(s) 684
BstKTI GATC 3 cut(s) 448, 603, 751
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 3 cut(s) 445, 600, 748
BstMWI GCNNNNNNNGC 2 cut(s) 162, 545
BstNI CCWGG 2 cut(s) 167, 753
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 5 cut(s) 117, 118, 165, 603, 751
BstV1I GCAGC 3 cut(s) 778, 781, 1153
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 3 cut(s) 445, 600, 748
BstYI RGATCY 3 cut(s) 445, 600, 748
Bsu36I CCTNAGG 1 cut(s) 1136
BsuI GTATCC 1 cut(s) 117
BsuRI GGCC 2 cut(s) 170, 756
BtsCI GGATG 1 cut(s) 990
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 1 cut(s) 1008
CciI TCATGA 2 cut(s) 175, 418
CfoI GCGC 1 cut(s) 684
Cfr13I GGNCC 2 cut(s) 30, 755
Cfr9I CCCGGG 1 cut(s) 118
Csp6I GTAC 4 cut(s) 218, 770, 947, 1129
CviAII CATG 4 cut(s) 176, 419, 811, 852
CviQI GTAC 4 cut(s) 218, 770, 947, 1129
DdeI CTNAG 9 cut(s) 9, 34, 249, 342, 924, 933, 1040, 1136, 1155
DpnI GATC 3 cut(s) 447, 602, 750
DpnII GATC 3 cut(s) 445, 600, 748
DraI TTTAAA 1 cut(s) 270
Eam1104I CTCTTC 6 cut(s) 204, 546, 612, 731, 861, 900
EarI CTCTTC 6 cut(s) 204, 546, 612, 731, 861, 900
Ecl136II GAGCTC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 915
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 654
Eco47I GGWCC 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 652
Eco57I CTGAAG 2 cut(s) 570, 846
Eco81I CCTNAGG 1 cut(s) 1136
Eco88I CYCGRG 2 cut(s) 118, 449
EcoICRI GAGCTC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 165, 751
EcoT14I CCWWGG 1 cut(s) 915
EcoT38I GRGCYC 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 915
FaeI CATG 4 cut(s) 179, 422, 814, 855
FalI AAGNNNNNCTT 4 cut(s) 668, 700, 1023, 1055
FatI CATG 4 cut(s) 175, 418, 810, 851
FauNDI CATATG 1 cut(s) 777
Fnu4HI GCNGC 4 cut(s) 154, 792, 795, 1142
FokI GGATG 1 cut(s) 997
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 4 cut(s) 154, 792, 795, 1142
FspBI CTAG 2 cut(s) 306, 578
GlaI GCGC 1 cut(s) 683
GluI GCNGC 4 cut(s) 154, 792, 795, 1142
HaeII RGCGCY 1 cut(s) 685
HaeIII GGCC 2 cut(s) 170, 756
HapII CCGG 2 cut(s) 119, 605
HhaI GCGC 1 cut(s) 684
Hin1II CATG 4 cut(s) 179, 422, 814, 855
Hin6I GCGC 1 cut(s) 682
HinP1I GCGC 1 cut(s) 682
HincII GTYRAC 2 cut(s) 691, 1036
HindII GTYRAC 2 cut(s) 691, 1036
HinfI GANTC 8 cut(s) 61, 132, 197, 230, 299, 587, 698, 928
HpaII CCGG 2 cut(s) 119, 605
HphI GGTGA 2 cut(s) 100, 1143
Hpy166II GTNNAC 4 cut(s) 263, 288, 691, 1036
Hpy188I TCNGA 7 cut(s) 250, 298, 391, 445, 865, 927, 936
Hpy188III TCNNGA 5 cut(s) 176, 419, 449, 451, 879
Hpy8I GTNNAC 4 cut(s) 263, 288, 691, 1036
HpyAV CCTTC 4 cut(s) 46, 80, 122, 220
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 6 cut(s) 539, 797, 902, 944, 1001, 1077
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 545
HpyF3I CTNAG 9 cut(s) 9, 34, 249, 342, 924, 933, 1040, 1136, 1155
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 4 cut(s) 179, 422, 814, 855
HspAI GCGC 1 cut(s) 682
Kzo9I GATC 3 cut(s) 445, 600, 748
LguI GCTCTTC 2 cut(s) 204, 546
LmnI GCTCC 2 cut(s) 657, 956
Lsp1109I GCAGC 3 cut(s) 778, 781, 1153
LweI GCATC 1 cut(s) 988
MaeI CTAG 2 cut(s) 306, 578
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 65
MalI GATC 3 cut(s) 447, 602, 750
MboI GATC 3 cut(s) 445, 600, 748
MflI RGATCY 3 cut(s) 445, 600, 748
MhlI GDGCHC 2 cut(s) 654, 961
MluCI AATT 4 cut(s) 639, 885, 1071, 1108
MlyI GAGTC 4 cut(s) 55, 191, 707, 937
MroXI GAANNNNTTC 1 cut(s) 586
MseI TTAA 4 cut(s) 269, 663, 827, 888
MspCI CTTAAG 1 cut(s) 826
MspI CCGG 2 cut(s) 119, 605
MspR9I CCNGG 5 cut(s) 119, 120, 167, 605, 753
MvaI CCWGG 2 cut(s) 167, 753
MwoI GCNNNNNNNGC 2 cut(s) 162, 545
NciI CCSGG 3 cut(s) 119, 120, 605
NdeI CATATG 1 cut(s) 777
NdeII GATC 3 cut(s) 445, 600, 748
NlaIII CATG 4 cut(s) 179, 422, 814, 855
NlaIV GGNNCC 4 cut(s) 255, 348, 602, 757
NspI RCATGY 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 449
PagI TCATGA 2 cut(s) 175, 418
PceI AGGCCT 1 cut(s) 170
PciI ACATGT 1 cut(s) 810
PciSI GCTCTTC 2 cut(s) 204, 546
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 4 cut(s) 132, 230, 299, 587
PfoI TCCNGGA 1 cut(s) 603
PkrI GCNGC 4 cut(s) 155, 793, 796, 1143
PleI GAGTC 4 cut(s) 55, 191, 706, 936
PpsI GAGTC 4 cut(s) 55, 191, 706, 936
PscI ACATGT 1 cut(s) 810
PsiI TTATAA 1 cut(s) 1113
Psp124BI GAGCTC 1 cut(s) 654
Psp1406I AACGTT 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 165, 751
PspGI CCWGG 2 cut(s) 165, 751
PspN4I GGNNCC 4 cut(s) 255, 348, 602, 757
PspPI GGNCC 2 cut(s) 30, 755
PsuI RGATCY 3 cut(s) 445, 600, 748
RsaI GTAC 4 cut(s) 219, 771, 948, 1130
RsaNI GTAC 4 cut(s) 218, 770, 947, 1129
SacI GAGCTC 1 cut(s) 654
SapI GCTCTTC 2 cut(s) 204, 546
SaqAI TTAA 4 cut(s) 269, 663, 827, 888
SatI GCNGC 4 cut(s) 154, 792, 795, 1142
Sau3AI GATC 3 cut(s) 445, 600, 748
Sau96I GGNCC 2 cut(s) 30, 755
ScaI AGTACT 1 cut(s) 948
SchI GAGTC 4 cut(s) 55, 191, 707, 937
ScrFI CCNGG 5 cut(s) 119, 120, 167, 605, 753
SduI GDGCHC 2 cut(s) 654, 961
SfaNI GCATC 1 cut(s) 988
Sfr274I CTCGAG 1 cut(s) 449
SinI GGWCC 1 cut(s) 30
SlaI CTCGAG 1 cut(s) 449
SmaI CCCGGG 1 cut(s) 120
SmlI CTYRAG 3 cut(s) 449, 695, 826
SmoI CTYRAG 3 cut(s) 449, 695, 826
Sse9I AATT 4 cut(s) 639, 885, 1071, 1108
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 2 cut(s) 153, 733
SspI AATATT 1 cut(s) 908
SspMI CTAG 2 cut(s) 306, 578
SstI GAGCTC 1 cut(s) 654
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 5 cut(s) 117, 118, 165, 603, 751
StyI CCWWGG 1 cut(s) 915
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 450, 815, 843
TasI AATT 4 cut(s) 639, 885, 1071, 1108
TatI WGTACW 4 cut(s) 217, 769, 946, 1128
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 4 cut(s) 132, 230, 299, 587
Tru1I TTAA 4 cut(s) 269, 663, 827, 888
Tru9I TTAA 4 cut(s) 269, 663, 827, 888
TscAI CASTG 1 cut(s) 1008
TseI GCWGC 3 cut(s) 791, 794, 1141
TspDTI ATGAA 3 cut(s) 192, 579, 1001
TspGWI ACGGA 2 cut(s) 969, 1003
TspMI CCCGGG 1 cut(s) 118
TspRI CASTG 1 cut(s) 1008
Vha464I CTTAAG 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 30
XapI RAATTY 1 cut(s) 1071
XceI RCATGY 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 449
XmaI CCCGGG 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 586
XspI CTAG 2 cut(s) 306, 578
ZrmI AGTACT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.