pycom01g07620

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013709
Physical Location & Seq
Forward (+)
8570936 .. 8572694
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07620.1

Sequence Viewer

Length: 618 bp
ATGATGGGTGCGGGTATGAACTTGATTACTACTGTGATAGGTTTTGGTATGAGTGCAACATTTATCGTGTTTGTTTGCACAAGAATCATCTGTGGGAGGCTGAGAGGTAGCGAATCGCGGCCTATGTTCGAGATTGAATCAAGGGTTGATCTTGAGCAGCCAGAGCATCAGGCTAGTGGCCTTGAACAAGTTGTGGTTTCTGCAATCCCTACGATGAAGTACGATCAAAAGGCTTTCAGTTCCTTAGAAGATGCGCAGTGCTCAATATGTTTGGGGGAGTACCAGGAGAAAGAAGTACTGCGAATCATGCCCAAATGTGGCCACAGTTTTCATCTCTCTTGCATTGATACATGGCTGAGGAAACAATCTACCTGCCCAGTTTGCCGTTTACCATTAAGAGAATCCCTCGGAACAAAACATGTGAGGTCAGCAACTTTTAGCGTGGCTCGATCTTTTGACAATTCTGAGATTTCAACTGAACATTCTCATCACTGGCTATTACCTGGCACAATTGAGCGTTCAGCAGGAAACGTTAGCAACCAAGGGCATTTAGAATCTGCTCCAGGAAACCCCTCTGAACCGACCCCATCTAGAGGAGAACCAGAAACAAGGCATTGA

Protein Analysis

206

Amino Acids

22.7

Weight (kDa)

6.3

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 85 - 129 8.6e-11 RING-H2 zinc finger domain
zf-RING_2 PF13639 86 - 129 1.5e-15 Ring finger domain
zf-RING_11 PF17123 86 - 115 2.2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 86 - 128 5.6e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 86 - 129 9.7e-07 zinc-RING finger domain
zf-C3HC4 PF00097 87 - 128 1.5e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 87 - 126 3.6e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05950 FvH4_7g13140 FvH4_7g13140
malus_domestica MD01G1051600.v1.1 MD07G1128400.v1.1
prunus_persica Prupe.2G154700_v2.0.a1
pyrus_communis pycom01g07620 pycom07g12420
rosa_chinensis RchiOBHm_Chr1g0352271
rosa_laevigata RLG00000028345
rosa_multiflora Rmu_sc0003774.1_g000003
rosa_roxburghii Rroxscaffold_4G00303050
rosa_rugosa Rorug01G0226300
rosa_samantha Rh1AG240700 Rh1BG211600 Rh1CG224500 Rh1DG238000
rosa_wichuraiana Rw1G020850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 380
AccII CGCG 1 cut(s) 118
AciI CCGC 2 cut(s) 11, 118
AclI AACGTT 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 319
AfaI GTAC 3 cut(s) 221, 281, 297
AfiI CCNNNNNNNGG 2 cut(s) 317, 593
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 3 cut(s) 137, 185, 474
AjnI CCWGG 3 cut(s) 282, 502, 562
Alw21I GWGCWC 1 cut(s) 263
AoxI GGCC 3 cut(s) 119, 178, 319
ApeKI GCWGC 1 cut(s) 157
AspLEI GCGC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 356
BbvI GCAGC 1 cut(s) 169
BccI CCATC 1 cut(s) 595
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 3 cut(s) 284, 504, 564
BfaI CTAG 2 cut(s) 174, 591
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 2 cut(s) 119, 158
BlsI GCNGC 2 cut(s) 120, 159
BmcAI AGTACT 1 cut(s) 297
Bme1390I CCNGG 3 cut(s) 284, 504, 564
BmrFI CCNGG 3 cut(s) 284, 504, 564
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 175, 241
BmuI ACTGGG 1 cut(s) 371
BplI GAGNNNNNCTC 2 cut(s) 390, 422
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 406, 541
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 593
Bse1I ACTGG 2 cut(s) 377, 497
BseBI CCWGG 3 cut(s) 284, 504, 564
BseDI CCNNGG 2 cut(s) 406, 541
BseLI CCNNNNNNNGG 2 cut(s) 317, 593
BseMII CTCAG 3 cut(s) 92, 347, 456
BseNI ACTGG 2 cut(s) 377, 497
BseRI GAGGAG 1 cut(s) 609
BseXI GCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 3 cut(s) 121, 180, 321
BsiHKAI GWGCWC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 317, 593
BsnI GGCC 3 cut(s) 121, 180, 321
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 3 cut(s) 148, 223, 449
BspACI CCGC 2 cut(s) 11, 118
BspANI GGCC 3 cut(s) 121, 180, 321
BspCNI CTCAG 3 cut(s) 93, 348, 457
BspFNI CGCG 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 380
BsrI ACTGG 2 cut(s) 377, 497
BssECI CCNNGG 2 cut(s) 406, 541
BssMI GATC 3 cut(s) 148, 223, 449
BssT1I CCWWGG 1 cut(s) 541
Bst2UI CCWGG 3 cut(s) 284, 504, 564
Bst4CI ACNGT 2 cut(s) 34, 326
BstDEI CTNAG 4 cut(s) 101, 244, 356, 465
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 151, 226, 452
BstMBI GATC 3 cut(s) 148, 223, 449
BstMWI GCNNNNNNNGC 3 cut(s) 163, 307, 381
BstNI CCWGG 3 cut(s) 284, 504, 564
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 3 cut(s) 282, 502, 562
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 169
BsuRI GGCC 3 cut(s) 121, 180, 321
BtsI GCAGTG 1 cut(s) 263
BtsIMutI CAGTG 2 cut(s) 263, 490
BveI ACCTGC 1 cut(s) 380
CfoI GCGC 1 cut(s) 256
Csp6I GTAC 3 cut(s) 220, 280, 296
CviAII CATG 3 cut(s) 307, 351, 419
CviQI GTAC 3 cut(s) 220, 280, 296
DdeI CTNAG 4 cut(s) 101, 244, 356, 465
DpnI GATC 3 cut(s) 150, 225, 451
DpnII GATC 3 cut(s) 148, 223, 449
EaeI YGGCCR 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 541
EcoRII CCWGG 3 cut(s) 282, 502, 562
EcoT14I CCWWGG 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 541
FaeI CATG 3 cut(s) 310, 354, 422
FaiI YATR 7 cut(s) 17, 50, 125, 268, 308, 352, 420
FatI CATG 3 cut(s) 306, 350, 418
FauI CCCGC 1 cut(s) 4
Fnu4HI GCNGC 2 cut(s) 119, 158
Fsp4HI GCNGC 2 cut(s) 119, 158
FspBI CTAG 2 cut(s) 174, 591
FspI TGCGCA 1 cut(s) 255
GlaI GCGC 1 cut(s) 255
GluI GCNGC 2 cut(s) 119, 158
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 3 cut(s) 121, 180, 321
HhaI GCGC 1 cut(s) 256
Hin1II CATG 3 cut(s) 310, 354, 422
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HinfI GANTC 6 cut(s) 84, 113, 137, 303, 401, 554
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 3 cut(s) 410, 466, 577
Hpy188III TCNNGA 3 cut(s) 130, 152, 591
Hpy8I GTNNAC 1 cut(s) 389
HpyCH4III ACNGT 2 cut(s) 34, 326
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 4 cut(s) 56, 78, 203, 342
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 307, 381
HpyF3I CTNAG 4 cut(s) 101, 244, 356, 465
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 3 cut(s) 310, 354, 422
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 3 cut(s) 148, 223, 449
LmnI GCTCC 1 cut(s) 565
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 2 cut(s) 175, 241
MaeI CTAG 2 cut(s) 174, 591
MaeII ACGT 1 cut(s) 531
MalI GATC 3 cut(s) 150, 225, 451
MboI GATC 3 cut(s) 148, 223, 449
MboII GAAGA 1 cut(s) 260
MfeI CAATTG 1 cut(s) 510
MhlI GDGCHC 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 2 cut(s) 460, 510
MluNI TGGCCA 1 cut(s) 321
MnlI CCTC 7 cut(s) 90, 98, 351, 416, 417, 583, 587
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MseI TTAA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 321
MspR9I CCNGG 3 cut(s) 284, 504, 564
MunI CAATTG 1 cut(s) 510
MvaI CCWGG 3 cut(s) 284, 504, 564
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 3 cut(s) 163, 307, 381
NdeII GATC 3 cut(s) 148, 223, 449
NlaIII CATG 3 cut(s) 310, 354, 422
NsbI TGCGCA 1 cut(s) 255
NspI RCATGY 1 cut(s) 422
PciI ACATGT 1 cut(s) 418
PfeI GAWTC 6 cut(s) 84, 113, 137, 303, 401, 554
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 2 cut(s) 120, 159
PscI ACATGT 1 cut(s) 418
Psp1406I AACGTT 1 cut(s) 531
Psp6I CCWGG 3 cut(s) 282, 502, 562
PspGI CCWGG 3 cut(s) 282, 502, 562
RsaI GTAC 3 cut(s) 221, 281, 297
RsaNI GTAC 3 cut(s) 220, 280, 296
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 2 cut(s) 119, 158
Sau3AI GATC 3 cut(s) 148, 223, 449
ScaI AGTACT 1 cut(s) 297
ScrFI CCNGG 3 cut(s) 284, 504, 564
SduI GDGCHC 1 cut(s) 263
SetI ASST 6 cut(s) 43, 109, 374, 428, 505, 534
SfaNI GCATC 2 cut(s) 175, 241
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 2 cut(s) 460, 510
SsiI CCGC 2 cut(s) 11, 118
SspMI CTAG 2 cut(s) 174, 591
StyD4I CCNGG 3 cut(s) 282, 502, 562
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 2 cut(s) 34, 326
TaiI ACGT 1 cut(s) 534
TaqI TCGA 2 cut(s) 129, 448
TasI AATT 2 cut(s) 460, 510
TatI WGTACW 1 cut(s) 295
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 6 cut(s) 84, 113, 137, 303, 401, 554
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 2 cut(s) 263, 497
TseI GCWGC 1 cut(s) 157
TspDTI ATGAA 3 cut(s) 32, 230, 320
TspRI CASTG 2 cut(s) 263, 497
XbaI TCTAGA 1 cut(s) 590
XceI RCATGY 1 cut(s) 422
XspI CTAG 2 cut(s) 174, 591
ZrmI AGTACT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.