RLG00000028345

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013709
Physical Location & Seq
Forward (+)
21611504 .. 21612827
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028345

Sequence Viewer

Length: 621 bp
ATGGGGTCGCATATGAACTTGGTTACTACTGTGATTGGTTTTGGTATGAGTGCAACATTCATTATATTTGTCTGCACAAGAATCATTTGTGGGAGGCTGAGAGGTGTTGAATCAAGGCCTATGTTCGAGATTGAATCAAGAACTGATCTTGAGCAGCCAGAGAATCGGTCTAGTGGCCTTGAACAAATTGTGGTGGCTGCAATCCCGACCATGAAGTATGATCAGGAGGCCTTTAGTTCTTTAGAAGATGCACAGTGTTCAATATGTCTGGGGGAGTACCAGGAGAAAGAAGTACTGAGAATTATGCCGAAATGTGGTCACAGTTTTCACCTCACTTGCATTGATATATGGCTGAGGAAACAGTCTACCTGTCCAGTTTGCCGTTTACCCTTGAAAGAATCTTTCGGACCAAAACATGTGAGATCAGCAACTTTTACTGTGGCTCGATCTTTTGATGATTCTGAGAACATTTCAACTCATGAACATTCTCAGCAGTGGCTATTACCGGCATCTGAACCAAATTCAGCAAGCAACAACATTAACAACCAAGTGCGGTTAGAACCTGCTCCTGGAAACCCCTCAGAACCTGGTCACTCAAGAGAACCAAACACAAGGCATTGA

Protein Analysis

207

Amino Acids

23.1

Weight (kDa)

6.06

Isoelectric Point (pI)

68.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 84 - 128 3.7e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 85 - 128 9.1e-15 Ring finger domain
zf-RING_11 PF17123 85 - 114 1.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 85 - 127 7.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 85 - 128 4.5e-06 zinc-RING finger domain
zf-C3HC4 PF00097 86 - 127 1.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 86 - 125 3.5e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05950 FvH4_7g13140 FvH4_7g13140
malus_domestica MD01G1051600.v1.1 MD07G1128400.v1.1
prunus_persica Prupe.2G154700_v2.0.a1
pyrus_communis pycom01g07620 pycom07g12420
rosa_chinensis RchiOBHm_Chr1g0352271
rosa_laevigata RLG00000028345
rosa_multiflora Rmu_sc0003774.1_g000003
rosa_roxburghii Rroxscaffold_4G00303050
rosa_rugosa Rorug01G0226300
rosa_samantha Rh1AG240700 Rh1BG211600 Rh1CG224500 Rh1DG238000
rosa_wichuraiana Rw1G020850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 571
AccI GTMKAC 1 cut(s) 365
AciI CCGC 1 cut(s) 553
AcsI RAATTY 1 cut(s) 520
AfaI GTAC 2 cut(s) 278, 294
AfiI CCNNNNNNNGG 2 cut(s) 314, 569
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 6 cut(s) 110, 134, 182, 261, 394, 474
AjnI CCWGG 3 cut(s) 279, 568, 586
AlwNI CAGNNNCTG 1 cut(s) 587
AoxI GGCC 3 cut(s) 116, 175, 228
ApeKI GCWGC 2 cut(s) 154, 197
ApoI RAATTY 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 407
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 166, 184
BceAI ACGGC 1 cut(s) 366
BciT130I CCWGG 3 cut(s) 281, 570, 588
BclI TGATCA 1 cut(s) 220
BfaI CTAG 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 571
BisI GCNGC 2 cut(s) 155, 198
BlsI GCNGC 2 cut(s) 156, 199
BmcAI AGTACT 1 cut(s) 294
Bme1390I CCNGG 3 cut(s) 281, 570, 588
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 407
BmrFI CCNGG 3 cut(s) 281, 570, 588
BmsI GCATC 2 cut(s) 238, 518
Bpu10I CCTNAGC 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 170, 580
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 569
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 1 cut(s) 374
BseBI CCWGG 3 cut(s) 281, 570, 588
BseLI CCNNNNNNNGG 2 cut(s) 314, 569
BseMII CTCAG 6 cut(s) 89, 287, 344, 453, 503, 594
BseNI ACTGG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 166, 184
BsgI GTGCAG 1 cut(s) 58
BshFI GGCC 3 cut(s) 118, 177, 230
BsiSI CCGG 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 314, 569
BsnI GGCC 3 cut(s) 118, 177, 230
Bsp143I GATC 4 cut(s) 145, 220, 422, 446
BspACI CCGC 1 cut(s) 553
BspANI GGCC 3 cut(s) 118, 177, 230
BspCNI CTCAG 6 cut(s) 90, 288, 345, 454, 502, 593
BspHI TCATGA 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 505
BsrI ACTGG 1 cut(s) 374
BssAI RCCGGY 1 cut(s) 505
BssMI GATC 4 cut(s) 145, 220, 422, 446
Bst2UI CCWGG 3 cut(s) 281, 570, 588
Bst4CI ACNGT 5 cut(s) 31, 255, 323, 363, 439
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 6 cut(s) 98, 296, 353, 462, 489, 580
BstKTI GATC 4 cut(s) 148, 223, 425, 449
BstMBI GATC 4 cut(s) 145, 220, 422, 446
BstNI CCWGG 3 cut(s) 281, 570, 588
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 3 cut(s) 279, 568, 586
BstV1I GCAGC 2 cut(s) 166, 184
BsuRI GGCC 3 cut(s) 118, 177, 230
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 2 cut(s) 260, 500
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 529
CaiI CAGNNNCTG 1 cut(s) 587
CciI TCATGA 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 407
CsiI ACCWGGT 1 cut(s) 586
Csp6I GTAC 2 cut(s) 277, 293
CviAII CATG 3 cut(s) 211, 416, 479
CviJI RGCY 9 cut(s) 97, 118, 157, 177, 197, 230, 352, 443, 499
CviKI_1 RGCY 9 cut(s) 97, 118, 157, 177, 197, 230, 352, 443, 499
CviQI GTAC 2 cut(s) 277, 293
DdeI CTNAG 6 cut(s) 98, 296, 353, 462, 489, 580
DpnI GATC 4 cut(s) 147, 222, 424, 448
DpnII GATC 4 cut(s) 145, 220, 422, 446
Eco147I AGGCCT 2 cut(s) 118, 230
Eco47I GGWCC 1 cut(s) 407
EcoRII CCWGG 3 cut(s) 279, 568, 586
FaeI CATG 3 cut(s) 214, 419, 482
FatI CATG 3 cut(s) 210, 415, 478
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 2 cut(s) 155, 198
Fsp4HI GCNGC 2 cut(s) 155, 198
FspBI CTAG 1 cut(s) 171
GluI GCNGC 2 cut(s) 155, 198
HaeIII GGCC 3 cut(s) 118, 177, 230
HapII CCGG 1 cut(s) 506
Hin1II CATG 3 cut(s) 214, 419, 482
HinfI GANTC 6 cut(s) 81, 110, 134, 163, 398, 458
HpaII CCGG 1 cut(s) 506
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 2 cut(s) 366, 386
Hpy188I TCNGA 4 cut(s) 407, 463, 514, 583
Hpy188III TCNNGA 7 cut(s) 127, 138, 149, 205, 224, 479, 597
Hpy8I GTNNAC 2 cut(s) 366, 386
HpyCH4III ACNGT 5 cut(s) 31, 255, 323, 363, 439
HpyCH4V TGCA 5 cut(s) 53, 75, 200, 251, 339
HpyF3I CTNAG 6 cut(s) 98, 296, 353, 462, 489, 580
Hsp92II CATG 3 cut(s) 214, 419, 482
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 4 cut(s) 145, 220, 422, 446
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 2 cut(s) 166, 184
LweI GCATC 2 cut(s) 238, 518
MabI ACCWGGT 1 cut(s) 586
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 22, 317, 590
MalI GATC 4 cut(s) 147, 222, 424, 448
MboI GATC 4 cut(s) 145, 220, 422, 446
MboII GAAGA 1 cut(s) 257
MluCI AATT 3 cut(s) 186, 300, 520
MnlI CCTC 6 cut(s) 87, 95, 220, 341, 348, 589
MseI TTAA 1 cut(s) 540
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 3 cut(s) 281, 570, 588
MvaI CCWGG 3 cut(s) 281, 570, 588
NdeI CATATG 1 cut(s) 12
NdeII GATC 4 cut(s) 145, 220, 422, 446
NlaIII CATG 3 cut(s) 214, 419, 482
NmuCI GTSAC 2 cut(s) 317, 590
NspI RCATGY 1 cut(s) 419
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 2 cut(s) 118, 230
PciI ACATGT 1 cut(s) 415
PfeI GAWTC 6 cut(s) 81, 110, 134, 163, 398, 458
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 2 cut(s) 156, 199
PscI ACATGT 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 279, 568, 586
PspGI CCWGG 3 cut(s) 279, 568, 586
PspPI GGNCC 1 cut(s) 407
PstNI CAGNNNCTG 1 cut(s) 587
RsaI GTAC 2 cut(s) 278, 294
RsaNI GTAC 2 cut(s) 277, 293
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 2 cut(s) 155, 198
Sau3AI GATC 4 cut(s) 145, 220, 422, 446
Sau96I GGNCC 1 cut(s) 407
ScaI AGTACT 1 cut(s) 294
ScrFI CCNGG 3 cut(s) 281, 570, 588
SetI ASST 5 cut(s) 106, 333, 371, 565, 589
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 2 cut(s) 238, 518
SinI GGWCC 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 149, 595
SmoI CTYRAG 2 cut(s) 149, 595
Sse9I AATT 3 cut(s) 186, 300, 520
SseBI AGGCCT 2 cut(s) 118, 230
SsiI CCGC 1 cut(s) 553
SspMI CTAG 1 cut(s) 171
StuI AGGCCT 2 cut(s) 118, 230
StyD4I CCNGG 3 cut(s) 279, 568, 586
TaaI ACNGT 5 cut(s) 31, 255, 323, 363, 439
TaqI TCGA 2 cut(s) 126, 445
TaqII GACCGA 1 cut(s) 156
TasI AATT 3 cut(s) 186, 300, 520
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 6 cut(s) 81, 110, 134, 163, 398, 458
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 2 cut(s) 260, 500
TseFI GTSAC 2 cut(s) 317, 590
TseI GCWGC 2 cut(s) 154, 197
Tsp45I GTSAC 2 cut(s) 317, 590
TspDTI ATGAA 4 cut(s) 29, 49, 227, 495
TspRI CASTG 2 cut(s) 260, 500
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 1 cut(s) 520
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 365
XspI CTAG 1 cut(s) 171
ZrmI AGTACT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.