pycom07g12420

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013709
Physical Location & Seq
Forward (+)
13761363 .. 13763366
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12420.1

Sequence Viewer

Length: 615 bp
ATGATGCGTTCGGTTATGAACTTGATTACTACTGTGATAGGTTTTGGTATGAGTGCAACATTTATTGTATTTGTTTGCACAAGAATCATCTGTGGGAGGCTGCGAGGTAGAGAATCGAGGCCTATGTTCGAGATTGAATCAAGGGTTGATCTTGAGCAGTCAGAGCATCAGGTTAGTGGCCTTGAACAAGTTGTGGTTTCTGCAATCCCTACGATGAAGTACGATCAAGAGGCTTTCAGTTCCTTAGAAGATGCGCAGTGCTCAATCTGTTTGGGGGAGTACCAGGAGAAAGAAGTACTGCGAATCATGCCCAAATGTGGCCACAGTTTTCATCTCTCTTGCATTGATACATGGCTGAGGAAACAGTCTACTTGCCCAGTTTGCCGTTTACCATTAAAAGAATCCCTTGGAACAAAACATGTGAGGTCAGCAACGTTCAGCATGGCTCGATCTTTTGACGAGTCTGAGATTTCAACTGAACATTCTCAGCAGTGGCTATTACCTGGCGCCACTGAGCGTTTGGCAGGCAACGTTAGCAACCAAGGGCATTTAGATTCTGCTCCAGGAAACCCCTCCGAACCGACCCCGTCTAGAGAACCAGAAACAAGGCAATGA

Protein Analysis

205

Amino Acids

22.85

Weight (kDa)

5.89

Isoelectric Point (pI)

64.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 85 - 129 8.6e-11 RING-H2 zinc finger domain
zf-RING_2 PF13639 86 - 129 1.4e-15 Ring finger domain
zf-RING_11 PF17123 86 - 115 2.2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 86 - 128 5.6e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 86 - 129 9.6e-07 zinc-RING finger domain
zf-C3HC4 PF00097 87 - 128 1.5e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 87 - 126 1.4e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05950 FvH4_7g13140 FvH4_7g13140
malus_domestica MD01G1051600.v1.1 MD07G1128400.v1.1
prunus_persica Prupe.2G154700_v2.0.a1
pyrus_communis pycom01g07620 pycom07g12420
rosa_chinensis RchiOBHm_Chr1g0352271
rosa_laevigata RLG00000028345
rosa_multiflora Rmu_sc0003774.1_g000003
rosa_roxburghii Rroxscaffold_4G00303050
rosa_rugosa Rorug01G0226300
rosa_samantha Rh1AG240700 Rh1BG211600 Rh1CG224500 Rh1DG238000
rosa_wichuraiana Rw1G020850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 368
AclI AACGTT 2 cut(s) 434, 531
AcoI YGGCCR 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 3 cut(s) 221, 281, 297
AfiI CCNNNNNNNGG 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 3 cut(s) 137, 185, 474
AjnI CCWGG 3 cut(s) 282, 502, 562
Alw21I GWGCWC 1 cut(s) 263
AoxI GGCC 3 cut(s) 119, 178, 319
ApeKI GCWGC 1 cut(s) 100
AspLEI GCGC 2 cut(s) 256, 509
BalI TGGCCA 1 cut(s) 321
BanI GGYRCC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 356
BbvI GCAGC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 3 cut(s) 284, 504, 564
BfaI CTAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 510
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
BmcAI AGTACT 1 cut(s) 297
Bme1390I CCNGG 3 cut(s) 284, 504, 564
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 3 cut(s) 284, 504, 564
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 175, 241
BmuI ACTGGG 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 173
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 406, 541
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 377
BseBI CCWGG 3 cut(s) 284, 504, 564
BseDI CCNNGG 2 cut(s) 406, 541
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMII CTCAG 4 cut(s) 347, 456, 500, 504
BseNI ACTGG 1 cut(s) 377
BseXI GCAGC 1 cut(s) 87
BshFI GGCC 3 cut(s) 121, 180, 321
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 317
BsnI GGCC 3 cut(s) 121, 180, 321
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 3 cut(s) 148, 223, 449
BspANI GGCC 3 cut(s) 121, 180, 321
BspCNI CTCAG 4 cut(s) 348, 457, 499, 505
BspLI GGNNCC 1 cut(s) 508
BspT107I GGYRCC 1 cut(s) 506
BsrI ACTGG 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 406, 541
BssMI GATC 3 cut(s) 148, 223, 449
BssNI GRCGYC 1 cut(s) 507
BssT1I CCWWGG 2 cut(s) 406, 541
Bst2UI CCWGG 3 cut(s) 284, 504, 564
Bst4CI ACNGT 3 cut(s) 34, 326, 366
BstACI GRCGYC 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 5 cut(s) 244, 356, 465, 486, 513
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 2 cut(s) 256, 509
BstKTI GATC 3 cut(s) 151, 226, 452
BstMBI GATC 3 cut(s) 148, 223, 449
BstMWI GCNNNNNNNGC 4 cut(s) 163, 307, 381, 534
BstNI CCWGG 3 cut(s) 284, 504, 564
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 3 cut(s) 282, 502, 562
BstV1I GCAGC 1 cut(s) 87
BsuRI GGCC 3 cut(s) 121, 180, 321
BtsI GCAGTG 2 cut(s) 263, 497
BtsIMutI CAGTG 3 cut(s) 263, 497, 510
Cac8I GCNNGC 1 cut(s) 526
CfoI GCGC 2 cut(s) 256, 509
Csp6I GTAC 3 cut(s) 220, 280, 296
CviAII CATG 4 cut(s) 307, 351, 419, 442
CviJI RGCY 8 cut(s) 100, 121, 180, 233, 321, 355, 446, 496
CviKI_1 RGCY 8 cut(s) 100, 121, 180, 233, 321, 355, 446, 496
CviQI GTAC 3 cut(s) 220, 280, 296
DdeI CTNAG 5 cut(s) 244, 356, 465, 486, 513
DinI GGCGCC 1 cut(s) 508
DpnI GATC 3 cut(s) 150, 225, 451
DpnII GATC 3 cut(s) 148, 223, 449
EaeI YGGCCR 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 406, 541
Eco147I AGGCCT 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 282, 502, 562
EcoT14I CCWWGG 2 cut(s) 406, 541
EgeI GGCGCC 1 cut(s) 508
EheI GGCGCC 1 cut(s) 508
ErhI CCWWGG 2 cut(s) 406, 541
FaeI CATG 4 cut(s) 310, 354, 422, 445
FaiI YATR 7 cut(s) 17, 50, 125, 308, 352, 420, 443
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 4 cut(s) 306, 350, 418, 441
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
FspBI CTAG 1 cut(s) 591
FspI TGCGCA 1 cut(s) 255
GlaI GCGC 2 cut(s) 255, 508
GluI GCNGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 3 cut(s) 121, 180, 321
HhaI GCGC 2 cut(s) 256, 509
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 4 cut(s) 310, 354, 422, 445
Hin6I GCGC 2 cut(s) 254, 507
HinP1I GCGC 2 cut(s) 254, 507
HinfI GANTC 7 cut(s) 84, 113, 137, 303, 401, 461, 554
Hpy166II GTNNAC 2 cut(s) 369, 389
Hpy188I TCNGA 3 cut(s) 163, 466, 577
Hpy188III TCNNGA 4 cut(s) 130, 152, 227, 591
Hpy8I GTNNAC 2 cut(s) 369, 389
HpyCH4III ACNGT 3 cut(s) 34, 326, 366
HpyCH4IV ACGT 2 cut(s) 434, 531
HpyCH4V TGCA 4 cut(s) 56, 78, 203, 342
HpyF10VI GCNNNNNNNGC 4 cut(s) 163, 307, 381, 534
HpyF3I CTNAG 5 cut(s) 244, 356, 465, 486, 513
HpySE526I ACGT 2 cut(s) 434, 531
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 4 cut(s) 310, 354, 422, 445
HspAI GCGC 2 cut(s) 254, 507
KasI GGCGCC 1 cut(s) 506
Kzo9I GATC 3 cut(s) 148, 223, 449
LmnI GCTCC 1 cut(s) 565
LpnPI CCDG 9 cut(s) 155, 269, 296, 390, 489, 510, 516, 549, 576
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 2 cut(s) 175, 241
MaeI CTAG 1 cut(s) 591
MaeII ACGT 2 cut(s) 434, 531
MalI GATC 3 cut(s) 150, 225, 451
MboI GATC 3 cut(s) 148, 223, 449
MboII GAAGA 1 cut(s) 260
MhlI GDGCHC 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 321
MluNI TGGCCA 1 cut(s) 321
Mly113I GGCGCC 1 cut(s) 507
MlyI GAGTC 1 cut(s) 470
MnlI CCTC 7 cut(s) 90, 98, 111, 223, 351, 417, 583
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MseI TTAA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 321
MspR9I CCNGG 3 cut(s) 284, 504, 564
MvaI CCWGG 3 cut(s) 284, 504, 564
MwoI GCNNNNNNNGC 4 cut(s) 163, 307, 381, 534
NarI GGCGCC 1 cut(s) 507
NdeII GATC 3 cut(s) 148, 223, 449
NlaIII CATG 4 cut(s) 310, 354, 422, 445
NlaIV GGNNCC 1 cut(s) 508
NsbI TGCGCA 1 cut(s) 255
NspI RCATGY 1 cut(s) 422
PceI AGGCCT 1 cut(s) 121
PciI ACATGT 1 cut(s) 418
PfeI GAWTC 6 cut(s) 84, 113, 137, 303, 401, 554
PflFI GACNNNGTC 1 cut(s) 586
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 1 cut(s) 102
PleI GAGTC 1 cut(s) 469
PluTI GGCGCC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 469
PscI ACATGT 1 cut(s) 418
Psp1406I AACGTT 2 cut(s) 434, 531
Psp6I CCWGG 3 cut(s) 282, 502, 562
PspGI CCWGG 3 cut(s) 282, 502, 562
PspN4I GGNNCC 1 cut(s) 508
PsyI GACNNNGTC 1 cut(s) 586
RsaI GTAC 3 cut(s) 221, 281, 297
RsaNI GTAC 3 cut(s) 220, 280, 296
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 3 cut(s) 148, 223, 449
ScaI AGTACT 1 cut(s) 297
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 3 cut(s) 284, 504, 564
SduI GDGCHC 1 cut(s) 263
SetI ASST 7 cut(s) 43, 109, 174, 428, 437, 505, 534
SfaNI GCATC 2 cut(s) 175, 241
SfoI GGCGCC 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SseBI AGGCCT 1 cut(s) 121
SspDI GGCGCC 1 cut(s) 506
SspMI CTAG 1 cut(s) 591
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 3 cut(s) 282, 502, 562
StyI CCWWGG 2 cut(s) 406, 541
TaaI ACNGT 3 cut(s) 34, 326, 366
TaiI ACGT 2 cut(s) 437, 534
TaqI TCGA 3 cut(s) 116, 129, 448
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 6 cut(s) 84, 113, 137, 303, 401, 554
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 3 cut(s) 263, 497, 517
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 3 cut(s) 32, 230, 320
TspRI CASTG 3 cut(s) 263, 497, 517
Tth111I GACNNNGTC 1 cut(s) 586
XbaI TCTAGA 1 cut(s) 590
XceI RCATGY 1 cut(s) 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 1 cut(s) 591
ZrmI AGTACT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.