pycom01g08280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008720
Physical Location & Seq
Forward (+)
9219245 .. 9221199
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g08280.2

Sequence Viewer

Length: 1344 bp
ATGAGGTTATACATAACCAATGATTTACAATCACAGATGATTAAGTTATCATCCCGGTATATTGATTTATCAATATCGAGGCTAAGTCCATCAACTTCTGTTTCTAAGGTGGCGTCAAAAGAAATTCAGCTGGAATCCAATTATGAGGCCCCAAAGTTTGCTCAGTCGATTTATCCATATCTTTTGGATCTACATAATATTTTTTCTGATCTCCCAGCAATGGGAAAGGCTCTAGCTAATGCTCAAAAGATGCTCTACAATGTGAACAGATGTGAAGCTGTAGATACCAATGTTATATCTGAATTCTATGCCCTTCAGATTGCAATCAAGGGGCTGAGAATTTCTCTGACCAATGCCGGTCACCTTCCCATTAATAAAATGAGGGAGCCGAGCTTAAATCAGGCTAAGCTTTCCGATCTTCCAGTTGAGGACAAGTCACATGCTCTGCTGGTAGAGGCCCTTCGTCGCCAGACTAAGAAGTTCGAGACCATAGTGGCAGTAGTGGATGCTAGTGGCTTAGCAGGTCTTAGAAAACACTGGAACACTCATGTCCCTTTAGATTTAGAGGTCAAGGAAATGGTCGGAAAGCTTGTTACTGATTGTGAGGGTGAGGGGGAAATGTCAAATGATACTGACAGGAAAAGACTGCTAACTGATAAACCTTTGGTGGCAGTAGGGGCAGGGGCAACAGCAGTTTTAGGAGCATCTTCACTCTCCAAAGCTGCGACACTCAAATTTCCTGCTTCAACCTTGATGAAAGTGGTGACTTTCAAAGTTCCAGCTTCACTTAAACTCTTTCTAAGACAAACCCATAAGACGGTGGGATTTGCTCTTAGTAAGACGCTTGGCCCCTCAAAAATGGTAGCTCCAGGTGTCAAATCAACAACTTTACTGAAAACAACTGCTTCTGCTGAGACGATCCGAGTAGCTGCCCATAGTGTTATAGGTGCTGCAGAGAAGACTAGCTTTTCAGCCATGAGAACAGCATTCTATCAAATAATGAGGAAACGACATATCCGGAAAATTGGTGTCCTACCGTGGGCTACCTTTGGGTGTAGTATTGCAACTTGTGCCGGATTGGTTGCGTACGGAGATGGGATTGAATGTGCTGCTGAATCTCTACCTGCAGCTCCATCATTTGCCAGTTTGGGTCCCGGGATTCAAAGTTTACACCTCGCATCTCAGGAAATATCACTGAGAGATAGCATGAAATTACAAAAATCTATTGAGTCCCTCATGTACAGATTGAAGAAAGTCAGAACTCAGTACAGGTGCAGTTCATGCACAAGCAAAGGTGGAGGCTGTGTAAATGTATGGATACCGTCCCAAGAAAAGGTAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

448

Amino Acids

48.57

Weight (kDa)

9.61

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 512, 1132
AccIII TCCGGA 1 cut(s) 1017
AclWI GGATC 2 cut(s) 195, 913
AcsI RAATTY 4 cut(s) 123, 302, 339, 734
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 3 cut(s) 1088, 1241, 1268
AfiI CCNNNNNNNGG 5 cut(s) 220, 221, 817, 1039, 1333
AgsI TTSAA 5 cut(s) 747, 772, 1103, 1163, 1249
AjnI CCWGG 1 cut(s) 868
Alw26I GTCTC 2 cut(s) 479, 908
AlwI GGATC 2 cut(s) 195, 913
Ama87I CYCGRG 1 cut(s) 1154
Aor13HI TCCGGA 1 cut(s) 1017
AoxI GGCC 3 cut(s) 147, 456, 847
ApeKI GCWGC 5 cut(s) 722, 929, 950, 1109, 1127
ApoI RAATTY 4 cut(s) 123, 302, 339, 734
AseI ATTAAT 1 cut(s) 372
AspS9I GGNCC 4 cut(s) 148, 457, 848, 1151
AsuC2I CCSGG 3 cut(s) 55, 1155, 1156
AsuHPI GGTGA 3 cut(s) 353, 620, 775
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 1 cut(s) 1151
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BarI GAAGNNNNNNTAC 2 cut(s) 444, 476
BbsI GAAGAC 1 cut(s) 965
BbvI GCAGC 5 cut(s) 709, 916, 937, 1096, 1139
BccI CCATC 3 cut(s) 97, 1088, 1141
BciT130I CCWGG 1 cut(s) 870
BciVI GTATCC 1 cut(s) 1311
BcnI CCSGG 3 cut(s) 55, 1155, 1156
BcoDI GTCTC 2 cut(s) 479, 908
BfaI CTAG 3 cut(s) 233, 510, 963
BfmI CTRYAG 3 cut(s) 279, 951, 1125
BfuAI ACCTGC 2 cut(s) 512, 1132
BfuI GTATCC 1 cut(s) 1311
BisI GCNGC 5 cut(s) 723, 930, 951, 1110, 1128
BlpI GCTNAGC 2 cut(s) 405, 517
BlsI GCNGC 5 cut(s) 724, 931, 952, 1111, 1129
Bme1390I CCNGG 4 cut(s) 55, 870, 1155, 1156
Bme18I GGWCC 1 cut(s) 1151
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 4 cut(s) 148, 457, 848, 1151
BmiI GGNNCC 5 cut(s) 150, 387, 850, 1152, 1153
BmrFI CCNGG 4 cut(s) 55, 870, 1155, 1156
BmsI GCATC 4 cut(s) 240, 496, 713, 1187
BpiI GAAGAC 1 cut(s) 965
BplI GAGNNNNNCTC 2 cut(s) 328, 360
BpmI CTGGAG 1 cut(s) 852
Bpu1102I GCTNAGC 2 cut(s) 405, 517
BpuMI CCSGG 3 cut(s) 55, 1155, 1156
BsaBI GATNNNNATC 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 1037, 1154
BsaWI WCCGGW 1 cut(s) 1017
BsaXI ACNNNNNCTCC 2 cut(s) 1290, 1320
Bsc4I CCNNNNNNNGG 5 cut(s) 220, 221, 817, 1039, 1333
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 3 cut(s) 422, 542, 1143
Bse3DI GCAATG 1 cut(s) 225
Bse8I GATNNNNATC 1 cut(s) 323
BseAI TCCGGA 1 cut(s) 1017
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 2 cut(s) 1037, 1154
BseGI GGATG 2 cut(s) 50, 511
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 5 cut(s) 220, 221, 817, 1039, 1333
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 6 cut(s) 176, 326, 903, 1187, 1196, 1277
BseNI ACTGG 3 cut(s) 422, 542, 1143
BseXI GCAGC 5 cut(s) 709, 916, 937, 1096, 1139
BseYI CCCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 1294
BshFI GGCC 3 cut(s) 149, 458, 849
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 5 cut(s) 55, 357, 1018, 1074, 1155
BsiWI CGTACG 1 cut(s) 1086
BslFI GGGAC 4 cut(s) 536, 1137, 1216, 1309
BslI CCNNNNNNNGG 5 cut(s) 220, 221, 817, 1039, 1333
BsmAI GTCTC 2 cut(s) 479, 908
BsmBI CGTCTC 1 cut(s) 908
BsmFI GGGAC 4 cut(s) 536, 1137, 1216, 1309
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 3 cut(s) 149, 458, 849
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 1154
Bsp13I TCCGGA 1 cut(s) 1017
Bsp1407I TGTACA 1 cut(s) 1239
Bsp143I GATC 4 cut(s) 187, 208, 415, 918
Bsp1720I GCTNAGC 2 cut(s) 405, 517
BspANI GGCC 3 cut(s) 149, 458, 849
BspCNI CTCAG 6 cut(s) 175, 327, 904, 1188, 1195, 1276
BspEI TCCGGA 1 cut(s) 1017
BspLI GGNNCC 5 cut(s) 150, 387, 850, 1152, 1153
BspMAI CTGCAG 2 cut(s) 955, 1129
BspMI ACCTGC 2 cut(s) 512, 1132
BspPI GGATC 2 cut(s) 195, 913
BspTNI GGTCTC 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 356
BsrGI TGTACA 1 cut(s) 1239
BsrI ACTGG 3 cut(s) 422, 542, 1143
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 1037, 1154
BssMI GATC 4 cut(s) 187, 208, 415, 918
BssNI GRCGYC 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 870
Bst4CI ACNGT 3 cut(s) 820, 1038, 1323
BstACI GRCGYC 1 cut(s) 113
BstAPI GCANNNNNTGC 2 cut(s) 1070, 1281
BstAUI TGTACA 1 cut(s) 1239
BstDSI CCRYGG 1 cut(s) 1037
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 2 cut(s) 50, 511
BstKTI GATC 4 cut(s) 190, 211, 418, 921
BstMAI GTCTC 2 cut(s) 479, 908
BstMBI GATC 4 cut(s) 187, 208, 415, 918
BstMWI GCNNNNNNNGC 3 cut(s) 677, 1070, 1281
BstNI CCWGG 1 cut(s) 870
BstNSI RCATGY 1 cut(s) 443
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 4 cut(s) 53, 868, 1153, 1154
BstSFI CTRYAG 3 cut(s) 279, 951, 1125
BstV1I GCAGC 5 cut(s) 709, 916, 937, 1096, 1139
BstV2I GAAGAC 1 cut(s) 965
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuI GTATCC 1 cut(s) 1311
BsuRI GGCC 3 cut(s) 149, 458, 849
BtgI CCRYGG 1 cut(s) 1037
BtsCI GGATG 2 cut(s) 50, 511
BtsIMutI CAGTG 2 cut(s) 535, 1193
BveI ACCTGC 2 cut(s) 512, 1132
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 4 cut(s) 148, 457, 848, 1151
Cfr9I CCCGGG 1 cut(s) 1154
CseI GACGC 2 cut(s) 102, 850
Csp6I GTAC 3 cut(s) 1087, 1240, 1267
CviAII CATG 6 cut(s) 440, 548, 976, 1207, 1237, 1281
CviQI GTAC 3 cut(s) 1087, 1240, 1267
DpnI GATC 4 cut(s) 189, 210, 417, 920
DpnII GATC 4 cut(s) 187, 208, 415, 918
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 1151
Eco57I CTGAAG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 1154
Eco91I GGTNACC 1 cut(s) 359
EcoO109I RGGNCCY 3 cut(s) 148, 457, 1151
EcoO65I GGTNACC 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 868
Esp3I CGTCTC 1 cut(s) 908
FaeI CATG 6 cut(s) 443, 551, 979, 1210, 1240, 1284
FalI AAGNNNNNCTT 2 cut(s) 950, 982
FaqI GGGAC 4 cut(s) 536, 1137, 1216, 1309
FatI CATG 6 cut(s) 439, 547, 975, 1206, 1236, 1280
Fnu4HI GCNGC 5 cut(s) 723, 930, 951, 1110, 1128
FokI GGATG 2 cut(s) 37, 518
Fsp4HI GCNGC 5 cut(s) 723, 930, 951, 1110, 1128
FspBI CTAG 3 cut(s) 233, 510, 963
GluI GCNGC 5 cut(s) 723, 930, 951, 1110, 1128
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 1 cut(s) 852
HaeIII GGCC 3 cut(s) 149, 458, 849
HapII CCGG 5 cut(s) 55, 357, 1018, 1074, 1155
HgaI GACGC 2 cut(s) 102, 850
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 6 cut(s) 443, 551, 979, 1210, 1240, 1284
HindIII AAGCTT 2 cut(s) 407, 587
HinfI GANTC 4 cut(s) 134, 1115, 1159, 1229
HpaII CCGG 5 cut(s) 55, 357, 1018, 1074, 1155
HphI GGTGA 3 cut(s) 353, 620, 775
Hpy166II GTNNAC 2 cut(s) 265, 1169
Hpy188I TCNGA 8 cut(s) 208, 301, 318, 348, 415, 584, 923, 1259
Hpy188III TCNNGA 3 cut(s) 484, 1018, 1184
Hpy8I GTNNAC 2 cut(s) 265, 1169
Hpy99I CGWCG 1 cut(s) 468
HpyAV CCTTC 3 cut(s) 323, 374, 470
HpyCH4III ACNGT 3 cut(s) 820, 1038, 1323
HpyCH4V TGCA 6 cut(s) 323, 953, 1064, 1127, 1275, 1284
HpyF10VI GCNNNNNNNGC 3 cut(s) 677, 1070, 1281
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 6 cut(s) 443, 551, 979, 1210, 1240, 1284
KflI GGGWCCC 1 cut(s) 1151
Kpn2I TCCGGA 1 cut(s) 1017
Kzo9I GATC 4 cut(s) 187, 208, 415, 918
LmnI GCTCC 4 cut(s) 385, 701, 871, 1135
Lsp1109I GCAGC 5 cut(s) 709, 916, 937, 1096, 1139
LweI GCATC 4 cut(s) 240, 496, 713, 1187
MaeI CTAG 3 cut(s) 233, 510, 963
MaeIII GTNAC 4 cut(s) 359, 435, 592, 763
MalI GATC 4 cut(s) 189, 210, 417, 920
MboI GATC 4 cut(s) 187, 208, 415, 918
MboII GAAGA 4 cut(s) 410, 699, 970, 1261
MflI RGATCY 1 cut(s) 187
MluCI AATT 7 cut(s) 123, 139, 302, 339, 734, 1023, 1211
MlyI GAGTC 1 cut(s) 1238
MmeI TCCRAC 1 cut(s) 562
MroI TCCGGA 1 cut(s) 1017
MseI TTAA 4 cut(s) 42, 372, 395, 789
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 5 cut(s) 55, 357, 1018, 1074, 1155
MspR9I CCNGG 4 cut(s) 55, 870, 1155, 1156
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 1 cut(s) 870
MwoI GCNNNNNNNGC 3 cut(s) 677, 1070, 1281
NciI CCSGG 3 cut(s) 55, 1155, 1156
NdeII GATC 4 cut(s) 187, 208, 415, 918
NlaIII CATG 6 cut(s) 443, 551, 979, 1210, 1240, 1284
NlaIV GGNNCC 5 cut(s) 150, 387, 850, 1152, 1153
NmeAIII GCCGAG 1 cut(s) 414
NmuCI GTSAC 3 cut(s) 359, 435, 763
NspI RCATGY 1 cut(s) 443
PctI GAATGC 1 cut(s) 986
PfeI GAWTC 3 cut(s) 134, 1115, 1159
Pfl23II CGTACG 1 cut(s) 1086
PkrI GCNGC 5 cut(s) 724, 931, 952, 1111, 1129
PleI GAGTC 1 cut(s) 1237
PpsI GAGTC 1 cut(s) 1237
PpuMI RGGWCCY 1 cut(s) 1151
PshBI ATTAAT 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 1151
Psp6I CCWGG 1 cut(s) 868
PspEI GGTNACC 1 cut(s) 359
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 1 cut(s) 868
PspLI CGTACG 1 cut(s) 1086
PspN4I GGNNCC 5 cut(s) 150, 387, 850, 1152, 1153
PspPI GGNCC 4 cut(s) 148, 457, 848, 1151
PspPPI RGGWCCY 1 cut(s) 1151
PstI CTGCAG 2 cut(s) 955, 1129
PsuI RGATCY 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 3 cut(s) 1088, 1241, 1268
RsaNI GTAC 3 cut(s) 1087, 1240, 1267
SaqAI TTAA 4 cut(s) 42, 372, 395, 789
SatI GCNGC 5 cut(s) 723, 930, 951, 1110, 1128
Sau3AI GATC 4 cut(s) 187, 208, 415, 918
Sau96I GGNCC 4 cut(s) 148, 457, 848, 1151
SchI GAGTC 1 cut(s) 1238
ScrFI CCNGG 4 cut(s) 55, 870, 1155, 1156
SfaNI GCATC 4 cut(s) 240, 496, 713, 1187
SfcI CTRYAG 3 cut(s) 279, 951, 1125
SinI GGWCC 1 cut(s) 1151
SmaI CCCGGG 1 cut(s) 1156
Sse9I AATT 7 cut(s) 123, 139, 302, 339, 734, 1023, 1211
SspI AATATT 1 cut(s) 199
SspMI CTAG 3 cut(s) 233, 510, 963
StyD4I CCNGG 4 cut(s) 53, 868, 1153, 1154
TaaI ACNGT 3 cut(s) 820, 1038, 1323
TaqI TCGA 3 cut(s) 77, 167, 483
TasI AATT 7 cut(s) 123, 139, 302, 339, 734, 1023, 1211
TatI WGTACW 2 cut(s) 1239, 1266
TfiI GAWTC 3 cut(s) 134, 1115, 1159
Tru1I TTAA 4 cut(s) 42, 372, 395, 789
Tru9I TTAA 4 cut(s) 42, 372, 395, 789
TscAI CASTG 2 cut(s) 542, 1200
TseFI GTSAC 3 cut(s) 359, 435, 763
TseI GCWGC 5 cut(s) 722, 929, 950, 1109, 1127
Tsp45I GTSAC 3 cut(s) 359, 435, 763
TspDTI ATGAA 3 cut(s) 770, 1223, 1269
TspGWI ACGGA 1 cut(s) 1104
TspMI CCCGGG 1 cut(s) 1154
TspRI CASTG 2 cut(s) 542, 1200
VpaK11BI GGWCC 1 cut(s) 1151
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 4 cut(s) 123, 302, 339, 734
XceI RCATGY 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 1154
XspI CTAG 3 cut(s) 233, 510, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.