pycom01g12770

Transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012966
Physical Location & Seq
Reverse (-)
13418429 .. 13425403
6975 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4608 bp
ATGGCGTCGAAGGGTAAGGGGATCGCCGCCGGAGGCGGATTCTCCGGCGGGAAGCGTAAGCACGATGACAAGTCCGGTGGTGGCCGGAAGAGGAGCAACCCCGGCGTCCTTAGATTCTTCGAAGACGTCGCTGACGAAGCCGACGACGGCGACGAGAGTGACTTCGATGACGATTTTATGGAAGAAGAATTTGAAACAGAACCTGTAGCCCAGAATGACCCAGGGAAAGCACATAACCTTCCATTCGTCCCTAAAGAGGAGGATCTGGATGGTGAAGAATTTGAAAAAATGATGGAAGAACGATACAGGAGTGGCTCCAGCTATGTAACCTATGCTGAAGATAATTATGAGAACAAAAGGTCTGTTGATGGAAATGTTCTGCCTACTGTTAAGGATCCAATCATCTGGAAAGTGAAATGCATGGTTGGCCGGGAGAGGCATTCAGCTTTCTGTATGATGCAGAAGTTTGTTGACTTCCGATCGCTAGGTACCAAACTACAGATTGTTTCTGCATTTGCTGTTGAGCACATCAAGGGTTTTGTTTTCATCGAAGCTGACAAGCAATCTGATATTAATGAGGCATGTAAAGGAATTTGTAGTATATACCCGTCCCGAGTAATGCCAGTTCCAAATAACGAAGTTTCTCATTTGCTGTCTCCTCGGACTAAATCCAATGGAATTACTGCGGGCATGTGGGCCCGTGTGAAGAATGGGAACTACAAGGGTGATTTGGCACAGGTTGTATTTGTGAACGATTTACGAAAAAGAGCGACTGTAAAGCTGATCCCCAGGATTGATCTTCAAGCAATGGCTGCAAAATTTGTAATAAATATCTTTCCTCTTTTTCACATGCTTTCCTCTTTCTGTTTGTTGGGTGGAGGAGGTACTAGAAAGAAAAGGCCTGCTGCTGCCCCAAGATTGATCAGCTCAAACGAACTCGAGGAGTTCCGCCCTCTCATCCAGTACAGGACTGATCGTGAAAGTGGCATGAAGTTTGAATTTCTTGATGGTCTGATGTTCAAGGATGGATATGTATACAAAAAAGTATCAATAGATTCATTAAGCTTCTGGGGTGTTATGCCATCAGAAGAAGAGCTACTGAAATTCACTCCTTCTGAGAACACCAAAAATGAGGACGAGGACTGGCTTACAGAGCTTTATGGTACAACAGAAAAGAAAAGGCGGACCATCAAAATTGAAGAAGGCGGTGGTAAAGGAGATGGTTCATCAGGTTCTGTGGGGAAAGGAGAGGGTTCATCAGGGTCTATGGGGAAAGGAGAGGGCACATCAGGGTCTATGGGGAAAGGAGAGGGCACATCAGGGTCTATGGGGAAAGGAGAGGGTTCATCACAGACTATGGGGAAAGGAGAGGGTTCATCTGGGTCTAAGGGGAAAGGAGAGGGTTCATCAGGTTCTATGGGAAAGGGTGAGGACTCATCAGCATCTATGGGGAAAGGAAAGGGTTCTTCAGTGTCCAGTGGGAATGGCTTTGAACTGTATGATCTTGTTTGTTTTGGCCGGAAAGATTTTGGTCTTGTAATAGGCATGGAGAAAGATGATAGCTACAAGATTCTGAAAGAGGGGGTGGAAGGACCTATTGTGCTGATGGTTCAGAAGCGTGAGTTAAAGAATGTTCTTTCTGATATGAAATTTACGGCATTAGATCGGCACATGAAGGCCATATGCGTTAGTGATACTGTCAAAGTGTTGGAAGGACCTTTGGAGGGTAGGCAGGGTATTGTTAAGCAAATCTATAGAGGCACCATATTCTTGTATGATGAGAACGAGACAGAAAATGGTGGTTATTTTTGCTCAAAATCCCAAATGTGTGAGAAAGTTAAGCTTTACATTGATTCTTGCAAAGAAAAGGTAGGAAGCTTTTTTCTTTTGTTCTTTGAGTGTATGGATGGGGATTCAGGTGCTCTGGGTTTTGGAGATTTCATGTCATCTCCTAAATCCCCGCTTTCGCCAAAAAAGCCATGGCAAGAAAGGGATAGTAACTTCAGTCAGGGTGATAAAGATGGAATGTTCTCAATTGGTCAAACTGTAAGAATCCGTGTGGGTCCATTGAAGGGATACCTCTGTCGCATCTTGGCTATACGCCGCGCTGATATTACTGTTAAGCTTGATTCTCAGCAAAAGGTTCTTACAGTTACAAAAGAGCATATTTCTGAAGTTCGTGGAAAGAGCTCTAATGTGCTGATTGGTAACTCTTGGGCTAGTGCAAATCTTGTTAAAAAAGATGATGATACTTCTTGGGGGAGCAAGGCGGCTCCAAGTACAGCTTCATCCTGGGGTGCAGCAGCAGCACCAACAGCGGATAACAATGATCAAGGTGCTGGCTGGGGAAAAAGTGACGCTTGGGGAAAATCAAGTTCTAAAACTGAGGGTGATAGTAGTGCACCAGATAACAGTGATCAAGGTGCTAGCTGGGGAAAAAGCAACACTTGGGGAAAATCAAGTGCTAAAGCTGGGGGTGACAGTAGTGCACCAGATAACAATGATCAAGGTGCTGGATGGGGAAAAAGCGATACTTGGGGAAAATCAAGTGCTAAAACTGGGGGTGGCAGCAGTGCACCAGCAGATAACAATGATCAAGGCGTTGGTTGGGGAAAAAGCGACTCTTGGGGGAAATCAAGTGCTAAAACTGGGGGTGACAGCGGTGCCCCAGATAGCAATGACCAAGGTGCTGGCTGGGGAAAAAGTGACTCTTGGGGGCAGAAGGCTGAACCTGCTGGGACGGGTCAACTGGATTCATGGGGCAAAGGTAAAAATGTTGTTGATGCGGGTGCATGGGGAAAGAAGTCTGATGCACCATCTGGTGATATTTCAAGCCCTGGGTGGGCTCAACAGAAATCTTGGGACAAAGGAAATGCAGTTGGTAGTGATGGGCCTGCTTGGGGTAAGCCTCAGAATAAAGCCACAGGGAACTGGAGCAGTAAAGATGAACCAACTGTTGGCGATGCAGGGTGGAAAAGCTCAACGCCTGCTGAAAATGTTCAGACTGGAAGCTGGGGTAAGGCAGGTGGTGGTTCAACCGAGAGTAAACAAGATGGCAGTTCTTCTTGGGGTAAGCCAGCTGGAGATTCTTCATGGGGTAAACAAACTGGAGGGTCTTCATGGGGTAAACAAGCTGATGCAACCGCTGCTGGAGTGGGGAACAATGGAGGCAAGTGGGGCAAAGGATCTACTGCCAATGAACAACAAAGTGGTGGAGGGGGAGATCAATCATCAGGCTGGGGTCAAACTAAAGCTTGGCAAGGTGGATCAAGTGGCGGAGGGGGAGATCAGGTTTCTAAATGGGGCCAGAAGGGCAATTGGAACTCAGGATCTAGTGAGACTGGTGGCAGTCAAGAATCTGGTTGGGGTAAGAAAAGCGATGGGAACATCGGATCTGCTTCCTCAGGTGGGAACCAGGATTCCACTTGGGGCAAGATAATCAACTCGAACTCAGGATCTGACGACACTGGAGGAAATCTTGATTCTAGTCGGGGGAAGAAGAGTACTTGGAATTCTCAATCTAGTGAGACTGGTGGCAATCAAGAGTCCAGTTGGGGGAAGAAAAGTGATTGGAAATCTGGATCTGATGACACCGGTGGGAACCAGGATTCAACTTGGGGCAAAAAAAGTAGTTGGAACTCAGGATCTGGTGATGCAGATCAGAAATCTGGTTGGGGCAGTAAAAGCAGTTGGAATTCAACAGATGGTGGGAATGGAGACCAGCCTGGGGATTCAAGTGGTGGGAATTGGAGAGGTGGACGTGGTTTTGGAGGTAGAGGCGGTGATAGAGATGGTGACAGAGGAGGTTTTGGAGATGGTGGGCGTAGAGGGGGTTTTGGGGGTAGAGGTGGAGGCGGGAGCAGTGGTGCTTGCTTCAAATGTGGTGAATCTGGGCACATGTCGAGGGAGTGCCCCCAGGGTGGTGGAGGTGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCGCGGGAGTGCCCTCAGGGCGGTGGAGGCGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCTGGACACATGTCGAGGGAGTGCCCACAGGGTGGTGGAGGTGGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCAAGGGAGTGCCCCAAGGGCGGCGGCGGCGGCAGTAAAGGTGGTGCTTGCTTCAAGTGTGGTGAGTCCGGGCACATGTCAAGGGAGTGCCCCCAGGGTGGTGGAGGCGGTGGAGGCGGTGCTTGCTACAAGTGTGGTGAGTCTGGGCACATGGCGAGGGACTGCTCCCAGGGCGGTGGAAGCTATGGTGGTGGCAGCCGTAGCGGTGGAGGCAGCGGTGGTGGTGCTTGCTTCAAGTGTGGCGAGTCTGGGCATATGGCAAGGGAGTGCCCCCAAGGCGGTGGAGGCGGTAGCAGTGGTGCTTGCTACAAGTGTGGTGAGTCAGGGCACATGGCGAGGGACTGCTCCCAGGGCGGTGGAGGCTATGGTGGTGGTGGCCGTGGCAGTGGCGGAGGTGGTGGCCGTGGCAGTGGTGGAGGCGGTGGCTGCTACAAGTGCGGACAGTCTGGCCATTTCGCAAGGGAGTGCCCGAACAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032044 GO:0032259 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spt5-NGN

Protein Analysis

1536

Amino Acids

158.61

Weight (kDa)

7.82

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt5-NGN PF03439 135 - 218 2.4e-19 Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5 PF23042 230 - 369 1.1e-22 Transcription elongation factor SPT5, KOW1 domain
KOWx_SPT5 PF23037 493 - 544 5.3e-10 Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5 PF23291 553 - 607 1.9e-24 Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5 PF23290 679 - 723 1e-07 Transcription elongation factor SPT5, fifth KOW domain
zf-CCHC PF00098 1290 - 1306 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1315 - 1331 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1340 - 1356 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1365 - 1381 9.3e-07 Zinc knuckle
zf-CCHC PF00098 1391 - 1407 8.2e-07 Zinc knuckle
zf-CCHC PF00098 1416 - 1432 5.5e-07 Zinc knuckle
zf-CCHC PF00098 1451 - 1467 4.3e-07 Zinc knuckle
zf-CCHC PF00098 1476 - 1492 5.5e-07 Zinc knuckle
zf-CCHC PF00098 1517 - 1533 8.6e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3014
AatII GACGTC 1 cut(s) 129
Acc36I ACCTGC 2 cut(s) 2740, 3014
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 3 cut(s) 488, 1760, 2663
AccB7I CCANNNNNTGG 2 cut(s) 2957, 4070
AccI GTMKAC 1 cut(s) 1035
AccII CGCG 2 cut(s) 2106, 3979
AcoI YGGCCR 6 cut(s) 82, 427, 1516, 4501, 4525, 4573
AcsI RAATTY 9 cut(s) 188, 278, 591, 818, 998, 1103, 1649, 3511, 3694
AcuI CTGAAG 4 cut(s) 357, 1452, 1987, 2193
AcyI GRCGYC 3 cut(s) 5, 105, 126
AdeI CACNNNGTG 1 cut(s) 4070
AfaI GTAC 6 cut(s) 490, 886, 965, 1165, 2281, 3505
AflIII ACRYGT 5 cut(s) 3897, 3972, 4047, 4122, 4200
AgeI ACCGGT 1 cut(s) 3593
AjiI CACGTC 1 cut(s) 3761
AjuI GAANNNNNNNTTGG 4 cut(s) 2269, 2301, 2940, 2972
AloI GAACNNNNNNTCC 2 cut(s) 3404, 3436
Alw21I GWGCWC 6 cut(s) 528, 1924, 2192, 2404, 2491, 2578
Alw26I GTCTC 5 cut(s) 660, 1781, 3332, 3521, 3711
Alw44I GTGCAC 3 cut(s) 2400, 2487, 2574
AlwNI CAGNNNCTG 4 cut(s) 203, 1235, 3458, 3647
Ama87I CYCGRG 2 cut(s) 612, 938
ApaI GGGCCC 1 cut(s) 700
ApaLI GTGCAC 3 cut(s) 2400, 2487, 2574
ApoI RAATTY 9 cut(s) 188, 278, 591, 818, 998, 1103, 1649, 3511, 3694
AseI ATTAAT 1 cut(s) 573
AsiGI ACCGGT 1 cut(s) 3593
Asp700I GAANNNNTTC 2 cut(s) 1462, 2997
Asp718I GGTACC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 2108
AspS9I GGNCC 8 cut(s) 696, 697, 1185, 1592, 1715, 2063, 2891, 3304
AsuC2I CCSGG 5 cut(s) 102, 431, 3967, 4117, 4195
AsuII TTCGAA 1 cut(s) 120
AsuNHI GCTAGC 1 cut(s) 2426
AvaI CYCGRG 2 cut(s) 612, 938
AvaII GGWCC 4 cut(s) 1185, 1592, 1715, 2063
AxyI CCTNAGG 2 cut(s) 3403, 3990
BaeI ACNNNNGTAYC 4 cut(s) 295, 328, 2068, 2101
BalI TGGCCA 1 cut(s) 4575
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 3 cut(s) 488, 1760, 2663
BanII GRGCYC 3 cut(s) 700, 2192, 2848
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BbsI GAAGAC 2 cut(s) 129, 3108
Bbv12I GWGCWC 6 cut(s) 528, 1924, 2192, 2404, 2491, 2578
BceAI ACGGC 5 cut(s) 163, 1671, 4308, 4488, 4512
BciVI GTATCC 1 cut(s) 2069
BclI TGATCA 5 cut(s) 921, 2329, 2416, 2503, 2593
BcnI CCSGG 5 cut(s) 102, 431, 3967, 4117, 4195
BcoDI GTCTC 5 cut(s) 660, 1781, 3332, 3521, 3711
BfaI CTAG 7 cut(s) 485, 888, 2220, 2427, 3333, 3486, 3522
BfmI CTRYAG 3 cut(s) 204, 497, 1753
BfuAI ACCTGC 2 cut(s) 2740, 3014
BfuI GTATCC 1 cut(s) 2069
BglI GCCNNNNNGGC 4 cut(s) 3312, 3993, 4143, 4401
BmcAI AGTACT 1 cut(s) 3505
Bme18I GGWCC 4 cut(s) 1185, 1592, 1715, 2063
BmeT110I CYCGRG 2 cut(s) 612, 938
BmgBI CACGTC 1 cut(s) 3761
BmgT120I GGNCC 8 cut(s) 696, 697, 1185, 1592, 1715, 2063, 2891, 3304
BmrI ACTGGG 2 cut(s) 2568, 2658
BmsI GCATC 8 cut(s) 447, 1451, 2097, 2773, 2800, 2953, 3127, 3643
BmtI GCTAGC 1 cut(s) 2430
BmuI ACTGGG 2 cut(s) 2568, 2658
BoxI GACNNNNGTC 1 cut(s) 4048
BpiI GAAGAC 2 cut(s) 129, 3108
BpmI CTGGAG 6 cut(s) 301, 2953, 3102, 3129, 3171, 3489
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 5 cut(s) 102, 431, 3967, 4117, 4195
BsaBI GATNNNNATC 1 cut(s) 3657
BsaHI GRCGYC 3 cut(s) 5, 105, 126
BsaI GGTCTC 1 cut(s) 3711
BsaWI WCCGGW 2 cut(s) 74, 3593
BsaXI ACNNNNNCTCC 4 cut(s) 3276, 3306, 3850, 3880
Bse118I RCCGGY 1 cut(s) 3593
Bse21I CCTNAGG 2 cut(s) 3403, 3990
Bse3DI GCAATG 2 cut(s) 813, 2683
Bse8I GATNNNNATC 1 cut(s) 3657
BseGI GGATG 6 cut(s) 274, 957, 1030, 1912, 2288, 2522
BseJI GATNNNNATC 1 cut(s) 3657
BseMI GCAATG 2 cut(s) 813, 2683
BseMII CTCAG 9 cut(s) 1107, 2147, 2376, 2924, 3339, 3417, 3465, 3654, 4004
BseRI GAGGAG 6 cut(s) 106, 272, 648, 894, 956, 3816
BseYI CCCAGC 7 cut(s) 2343, 2430, 2471, 2694, 2735, 3012, 3237
BsgI GTGCAG 1 cut(s) 2319
Bsh1236I CGCG 2 cut(s) 2106, 3979
Bsh1285I CGRYCG 1 cut(s) 482
BshNI GGYRCC 3 cut(s) 488, 1760, 2663
BshTI ACCGGT 1 cut(s) 3593
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 6 cut(s) 528, 1924, 2192, 2404, 2491, 2578
BsiHKCI CYCGRG 2 cut(s) 612, 938
BslFI GGGAC 6 cut(s) 233, 595, 2752, 2876, 4298, 4478
BsmAI GTCTC 5 cut(s) 660, 1781, 3332, 3521, 3711
BsmFI GGGAC 6 cut(s) 233, 595, 2752, 2876, 4298, 4478
BsmI GAATGC 1 cut(s) 439
Bso31I GGTCTC 1 cut(s) 3711
BsoBI CYCGRG 2 cut(s) 612, 938
Bsp119I TTCGAA 1 cut(s) 120
Bsp120I GGGCCC 1 cut(s) 696
Bsp19I CCATGG 1 cut(s) 1979
BspCNI CTCAG 9 cut(s) 1108, 2146, 2377, 2923, 3338, 3416, 3464, 3653, 4003
BspFNI CGCG 2 cut(s) 2106, 3979
BspMI ACCTGC 2 cut(s) 2740, 3014
BspOI GCTAGC 1 cut(s) 2430
BspQI GCTCTTC 1 cut(s) 1086
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 3 cut(s) 488, 1760, 2663
BspTNI GGTCTC 1 cut(s) 3711
BsrDI GCAATG 2 cut(s) 813, 2683
BsrFI RCCGGY 1 cut(s) 3593
BssAI RCCGGY 1 cut(s) 3593
BssNAI GTATAC 1 cut(s) 1036
BssNI GRCGYC 3 cut(s) 5, 105, 126
BssT1I CCWWGG 4 cut(s) 1979, 2683, 4140, 4399
Bst1107I GTATAC 1 cut(s) 1036
Bst6I CTCTTC 3 cut(s) 83, 1086, 3494
BstACI GRCGYC 3 cut(s) 5, 105, 126
BstAPI GCANNNNNTGC 2 cut(s) 812, 3146
BstBI TTCGAA 1 cut(s) 120
BstDSI CCRYGG 3 cut(s) 1979, 4504, 4528
BstF5I GGATG 6 cut(s) 274, 957, 1030, 1912, 2288, 2522
BstFNI CGCG 2 cut(s) 2106, 3979
BstHHI GCGC 1 cut(s) 2108
BstMAI GTCTC 5 cut(s) 660, 1781, 3332, 3521, 3711
BstMCI CGRYCG 1 cut(s) 482
BstNSI RCATGY 8 cut(s) 585, 694, 853, 3901, 3976, 4051, 4126, 4204
BstPAI GACNNNNGTC 1 cut(s) 4048
BstSFI CTRYAG 3 cut(s) 204, 497, 1753
BstUI CGCG 2 cut(s) 2106, 3979
BstV2I GAAGAC 2 cut(s) 129, 3108
BstX2I RGATCY 8 cut(s) 262, 394, 3185, 3329, 3392, 3455, 3581, 3644
BstXI CCANNNNNNTGG 7 cut(s) 405, 2690, 3923, 4226, 4301, 4406, 4481
BstYI RGATCY 8 cut(s) 262, 394, 3185, 3329, 3392, 3455, 3581, 3644
BstZ17I GTATAC 1 cut(s) 1036
Bsu36I CCTNAGG 2 cut(s) 3403, 3990
BsuI GTATCC 1 cut(s) 2069
BtgI CCRYGG 3 cut(s) 1979, 4504, 4528
BtgZI GCGATG 2 cut(s) 2976, 3393
BtrI CACGTC 1 cut(s) 3761
BtsCI GGATG 6 cut(s) 274, 957, 1030, 1912, 2288, 2522
BtsI GCAGTG 5 cut(s) 2578, 3868, 4426, 4516, 4540
BveI ACCTGC 2 cut(s) 2740, 3014
CaiI CAGNNNCTG 4 cut(s) 203, 1235, 3458, 3647
CfoI GCGC 1 cut(s) 2108
Cfr10I RCCGGY 1 cut(s) 3593
Cfr13I GGNCC 8 cut(s) 696, 697, 1185, 1592, 1715, 2063, 2891, 3304
CseI GACGC 2 cut(s) 94, 2366
Csp6I GTAC 6 cut(s) 489, 885, 964, 1164, 2280, 3504
CspAI ACCGGT 1 cut(s) 3593
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviQI GTAC 6 cut(s) 489, 885, 964, 1164, 2280, 3504
DraIII CACNNNGTG 1 cut(s) 4070
EaeI YGGCCR 6 cut(s) 82, 427, 1516, 4501, 4525, 4573
Eam1104I CTCTTC 3 cut(s) 83, 1086, 3494
EarI CTCTTC 3 cut(s) 83, 1086, 3494
EciI GGCGGA 5 cut(s) 51, 938, 1198, 3291, 4530
Ecl136II GAGCTC 1 cut(s) 2190
Eco130I CCWWGG 4 cut(s) 1979, 2683, 4140, 4399
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 3 cut(s) 700, 2192, 2848
Eco31I GGTCTC 1 cut(s) 3711
Eco47I GGWCC 4 cut(s) 1185, 1592, 1715, 2063
Eco53kI GAGCTC 1 cut(s) 2190
Eco57I CTGAAG 4 cut(s) 357, 1452, 1987, 2193
Eco81I CCTNAGG 2 cut(s) 3403, 3990
Eco88I CYCGRG 2 cut(s) 612, 938
EcoICRI GAGCTC 1 cut(s) 2190
EcoO109I RGGNCCY 2 cut(s) 1592, 1715
EcoRI GAATTC 2 cut(s) 3511, 3694
EcoT14I CCWWGG 4 cut(s) 1979, 2683, 4140, 4399
EcoT22I ATGCAT 1 cut(s) 422
EcoT38I GRGCYC 3 cut(s) 700, 2192, 2848
ErhI CCWWGG 4 cut(s) 1979, 2683, 4140, 4399
FaqI GGGAC 6 cut(s) 233, 595, 2752, 2876, 4298, 4478
FauI CCCGC 6 cut(s) 41, 679, 1968, 2779, 3848, 3972
FauNDI CATATG 2 cut(s) 1682, 4380
FbaI TGATCA 5 cut(s) 921, 2329, 2416, 2503, 2593
FblI GTMKAC 1 cut(s) 1035
FokI GGATG 6 cut(s) 281, 944, 1037, 1919, 2275, 2529
FriOI GRGCYC 3 cut(s) 700, 2192, 2848
FspBI CTAG 7 cut(s) 485, 888, 2220, 2427, 3333, 3486, 3522
GlaI GCGC 1 cut(s) 2107
GsaI CCCAGC 7 cut(s) 2347, 2434, 2475, 2698, 2739, 3016, 3241
GsuI CTGGAG 6 cut(s) 301, 2953, 3102, 3129, 3171, 3489
HgaI GACGC 2 cut(s) 94, 2366
HhaI GCGC 1 cut(s) 2108
Hin1I GRCGYC 3 cut(s) 5, 105, 126
Hin6I GCGC 1 cut(s) 2106
HinP1I GCGC 1 cut(s) 2106
HincII GTYRAC 2 cut(s) 472, 2747
HindII GTYRAC 2 cut(s) 472, 2747
HindIII AAGCTT 5 cut(s) 1063, 1841, 1876, 2123, 3252
Hpy99I CGWCG 5 cut(s) 10, 131, 146, 149, 155
HpyCH4IV ACGT 2 cut(s) 126, 3760
HpySE526I ACGT 2 cut(s) 126, 3760
Hsp92I GRCGYC 3 cut(s) 5, 105, 126
HspAI GCGC 1 cut(s) 2106
KpnI GGTACC 1 cut(s) 492
Ksp22I TGATCA 5 cut(s) 921, 2329, 2416, 2503, 2593
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 8 cut(s) 93, 320, 2262, 2278, 2934, 3858, 4295, 4475
LweI GCATC 8 cut(s) 447, 1451, 2097, 2773, 2800, 2953, 3127, 3643
MaeI CTAG 7 cut(s) 485, 888, 2220, 2427, 3333, 3486, 3522
MaeII ACGT 2 cut(s) 126, 3760
MfeI CAATTG 2 cut(s) 2034, 3316
MflI RGATCY 8 cut(s) 262, 394, 3185, 3329, 3392, 3455, 3581, 3644
MlsI TGGCCA 1 cut(s) 4575
MluNI TGGCCA 1 cut(s) 4575
MmeI TCCRAC 3 cut(s) 1689, 3614, 3671
Mox20I TGGCCA 1 cut(s) 4575
Mph1103I ATGCAT 1 cut(s) 422
MroXI GAANNNNTTC 2 cut(s) 1462, 2997
MscI TGGCCA 1 cut(s) 4575
MseI TTAA 9 cut(s) 390, 573, 1061, 1625, 1743, 1839, 2121, 2235, 4606
Msp20I TGGCCA 1 cut(s) 4575
MspA1I CMGCKG 5 cut(s) 2318, 2661, 3080, 3146, 4341
MunI CAATTG 2 cut(s) 2034, 3316
Mva1269I GAATGC 1 cut(s) 439
MvnI CGCG 2 cut(s) 2106, 3979
NciI CCSGG 5 cut(s) 102, 431, 3967, 4117, 4195
NcoI CCATGG 1 cut(s) 1979
NdeI CATATG 2 cut(s) 1682, 4380
NheI GCTAGC 1 cut(s) 2426
NmuCI GTSAC 6 cut(s) 158, 2354, 2477, 2654, 2705, 3794
NsiI ATGCAT 1 cut(s) 422
NspI RCATGY 8 cut(s) 585, 694, 853, 3901, 3976, 4051, 4126, 4204
NspV TTCGAA 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 938
PaqCI CACCTGC 1 cut(s) 3014
PasI CCCWGGG 6 cut(s) 221, 2837, 3916, 4219, 4294, 4474
PceI AGGCCT 1 cut(s) 901
PciI ACATGT 5 cut(s) 3897, 3972, 4047, 4122, 4200
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 141, 150
PctI GAATGC 1 cut(s) 439
PdmI GAANNNNTTC 2 cut(s) 1462, 2997
PflMI CCANNNNNTGG 2 cut(s) 2957, 4070
PinAI ACCGGT 1 cut(s) 3593
Ple19I CGATCG 1 cut(s) 482
PpuMI RGGWCCY 2 cut(s) 1592, 1715
PscI ACATGT 5 cut(s) 3897, 3972, 4047, 4122, 4200
PshAI GACNNNNGTC 1 cut(s) 4048
PshBI ATTAAT 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 2192
Psp5II RGGWCCY 2 cut(s) 1592, 1715
PspFI CCCAGC 7 cut(s) 2343, 2430, 2471, 2694, 2735, 3012, 3237
PspOMI GGGCCC 1 cut(s) 696
PspPI GGNCC 8 cut(s) 696, 697, 1185, 1592, 1715, 2063, 2891, 3304
PspPPI RGGWCCY 2 cut(s) 1592, 1715
PspXI VCTCGAGB 1 cut(s) 938
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PstNI CAGNNNCTG 4 cut(s) 203, 1235, 3458, 3647
PsuI RGATCY 8 cut(s) 262, 394, 3185, 3329, 3392, 3455, 3581, 3644
PvuI CGATCG 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 3080
RsaI GTAC 6 cut(s) 490, 886, 965, 1165, 2281, 3505
RsaNI GTAC 6 cut(s) 489, 885, 964, 1164, 2280, 3504
SacI GAGCTC 1 cut(s) 2192
SapI GCTCTTC 1 cut(s) 1086
SaqAI TTAA 9 cut(s) 390, 573, 1061, 1625, 1743, 1839, 2121, 2235, 4606
Sau96I GGNCC 8 cut(s) 696, 697, 1185, 1592, 1715, 2063, 2891, 3304
ScaI AGTACT 1 cut(s) 3505
SfaNI GCATC 8 cut(s) 447, 1451, 2097, 2773, 2800, 2953, 3127, 3643
SfcI CTRYAG 3 cut(s) 204, 497, 1753
Sfr274I CTCGAG 1 cut(s) 938
SfuI TTCGAA 1 cut(s) 120
SgrAI CRCCGGYG 1 cut(s) 3593
SinI GGWCC 4 cut(s) 1185, 1592, 1715, 2063
SlaI CTCGAG 1 cut(s) 938
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
SseBI AGGCCT 1 cut(s) 901
SspMI CTAG 7 cut(s) 485, 888, 2220, 2427, 3333, 3486, 3522
SstI GAGCTC 1 cut(s) 2192
StuI AGGCCT 1 cut(s) 901
StyI CCWWGG 4 cut(s) 1979, 2683, 4140, 4399
TaiI ACGT 2 cut(s) 129, 3763
TaqI TCGA 8 cut(s) 8, 120, 165, 549, 939, 3446, 3902, 4052
TatI WGTACW 3 cut(s) 963, 2279, 3503
TauI GCSGC 7 cut(s) 29, 2106, 2273, 4149, 4152, 4155, 4158
Tru1I TTAA 9 cut(s) 390, 573, 1061, 1625, 1743, 1839, 2121, 2235, 4606
Tru9I TTAA 9 cut(s) 390, 573, 1061, 1625, 1743, 1839, 2121, 2235, 4606
TseFI GTSAC 6 cut(s) 158, 2354, 2477, 2654, 2705, 3794
Tsp45I GTSAC 6 cut(s) 158, 2354, 2477, 2654, 2705, 3794
TspGWI ACGGA 1 cut(s) 2045
Van91I CCANNNNNTGG 2 cut(s) 2957, 4070
VneI GTGCAC 3 cut(s) 2400, 2487, 2574
VpaK11BI GGWCC 4 cut(s) 1185, 1592, 1715, 2063
VspI ATTAAT 1 cut(s) 573
XapI RAATTY 9 cut(s) 188, 278, 591, 818, 998, 1103, 1649, 3511, 3694
XceI RCATGY 8 cut(s) 585, 694, 853, 3901, 3976, 4051, 4126, 4204
XcmI CCANNNNNNNNNTGG 1 cut(s) 1977
XhoI CTCGAG 1 cut(s) 938
XmiI GTMKAC 1 cut(s) 1035
XmnI GAANNNNTTC 2 cut(s) 1462, 2997
XspI CTAG 7 cut(s) 485, 888, 2220, 2427, 3333, 3486, 3522
ZraI GACGTC 1 cut(s) 127
ZrmI AGTACT 1 cut(s) 3505
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.