pycom01g14160

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014410
Physical Location & Seq
Reverse (-)
14416399 .. 14419462
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1446 bp
ATGGTAAATAGTGGGAAAATCGTACTTAAAAATTGTCCATGGATAAACCCCACGAGTAGCCAGCAGGAACTCAGTAGCAACTCGCAACTCAGTCTTCTTCTCCAAAACCCCCAAAACCCCGAAACCAAAATGCGGCGCGCCATAGACGCCCACCTCAAGGCGGCGCGTCTCTTTCCCTCCTTATCATACTTCTCTTCTTCCTCCTCACCGTCTACGCCTCGCGTCACCGTCAGTGCAATCCTAACCCTAGCTCATCCCCACGCTCCTTCCATTTCCAAAACGCTGAACCTCCACTCGATAACAATTTCCTCCTGCTCTTCGATTCTCAGAAACCACGGCGTCGCCTCCAATTTTGGCCTCTCCGTCGTGCGCTGCGTTTCGTCGGTCCCGCATGCCGATACATTGGAGTGGAACGAGCCGGTTTCGTGCTCGGAGGTCGGCGATGGCCACAAAGCCCCCGTGGACGAAGACTCCAAGCCCTCGATTCCTGTCCGAGCATACTTCTTCTCCACCAGTGTCGATTTAAGAAGCTTGGTAGAGCAGAACAGGCTGAATTTCATCCCACCTACATCGCGAATGACTAATTATGTTGTTTTTAAGTTCGGGGAGCTTTCCGATTCTAACGATGGTTTGGGCCTTAGTTTAAGTGGGAGTGACTGTTGCTACATGGTAGTCTTTCAGTATGGCTCCATTGTCTTGTTCAATGTCCGTGAAAAAGATATTGATGGGTATTTGAAAATCGTGGAAAAACACGCATCACGATCGCTGCCTGAAATGAGAAAGGATGAGTATGAGGTAAGAGAGAACCCCACTTTGCACACATGGATGGAAGGTGGATTAGATTACATAATGTTGCAGTACTTGAATGTTGATGGAATCCGTACTATTGGTAGTGTTTTAGGTCAAAGTATTGCGCTTGATTACTATGTGCGCCAGGTTGATGGAATGGTTGCCGAGTTTACGGACATTAATCGTGAAATGGAAACTACTGGGAAGTTTAAAATGAAGAGGAAGAAACTGTTTCAGTTAGTGGGAAAGGCAAATTCTAATCTTGCTGATGTGATTCTTAAGCTCGGACTTTTTGAGAGATCGGATATTGCCTGGAAGGATGCCAAATATGCACAGATATGGGAATATCTCAGGGATGAATTTGAGTTGACTCAGAGATTTGCAAGTCTTGATTTTAAGTTGAAGTTTGTGGAGGTACACAAAACAAATTATTCTCAATACTGGTTTACTAAATCTGGTGAACTACTTTTCAACTGCTGTGGATGTTGTGCTGACATCTTGTTTTCTTCGTTGCAGCACAATATTCGTTTTCTTCAGGAAATTCTTCAAAACAGGAAGTCAGATTTCTTGGAATGGCTCATCATTGCGTTAATAGGTGCTGAAATTCTTCTCTCCCTTTACGACATTGTCCACAGGTCGGCACTTGCTTCGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

482

Amino Acids

54.29

Weight (kDa)

6.65

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 223 - 393 2.1e-34 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13610
fragaria_vesca FvH4_7g20360
malus_domestica MD01G1113300.v1.1 MD07G1180700.v1.1
prunus_persica Prupe.2G220000_v2.0.a1
pyrus_communis pycom01g14160 pycom07g17400
rosa_chinensis RchiOBHm_Chr1g0364321
rosa_laevigata RLG00000027495
rosa_multiflora Rmu_sc0007470.1_g000002
rosa_roxburghii Rroxscaffold_4G00292430
rosa_rugosa Rorug01G0315400
rosa_samantha Rh1AG323100 Rh1BG285700 Rh1CG302400 Rh1DG317200
rosa_wichuraiana Rw1G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 212
AccII CGCG 4 cut(s) 138, 166, 222, 574
AciI CCGC 3 cut(s) 133, 161, 389
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 5 cut(s) 553, 1042, 1148, 1329, 1392
AcuI CTGAAG 1 cut(s) 1307
AcyI GRCGYC 2 cut(s) 147, 339
AfaI GTAC 4 cut(s) 24, 860, 883, 1206
AfiI CCNNNNNNNGG 4 cut(s) 132, 157, 160, 1426
AflII CTTAAG 1 cut(s) 1067
AgsI TTSAA 6 cut(s) 703, 736, 865, 1192, 1261, 1337
AjnI CCWGG 2 cut(s) 933, 1100
AluBI AGCT 4 cut(s) 251, 531, 610, 1072
AluI AGCT 4 cut(s) 251, 531, 610, 1072
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 1 cut(s) 173
AoxI GGCC 3 cut(s) 355, 445, 634
ApeKI GCWGC 3 cut(s) 372, 766, 1303
ApoI RAATTY 5 cut(s) 553, 1042, 1148, 1329, 1392
AscI GGCGCGCC 1 cut(s) 136
AseI ATTAAT 1 cut(s) 969
Asp700I GAANNNNTTC 2 cut(s) 1332, 1395
AspLEI GCGC 6 cut(s) 138, 140, 166, 372, 916, 933
AspS9I GGNCC 2 cut(s) 385, 634
AsuHPI GGTGA 3 cut(s) 198, 217, 1259
AvaII GGWCC 1 cut(s) 385
BalI TGGCCA 1 cut(s) 447
BauI CACGAG 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 86, 474
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 3 cut(s) 359, 753, 1315
BccI CCATC 6 cut(s) 437, 620, 719, 820, 866, 935
BceAI ACGGC 1 cut(s) 352
BcgI CGANNNNNNTGC 5 cut(s) 744, 778, 1295, 1329, 1419
BciT130I CCWGG 2 cut(s) 935, 1102
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 1 cut(s) 248
BfrI CTTAAG 1 cut(s) 1067
BisI GCNGC 5 cut(s) 134, 162, 373, 767, 1304
BlsI GCNGC 5 cut(s) 135, 163, 374, 768, 1305
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 2 cut(s) 935, 1102
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 2 cut(s) 385, 634
BmiI GGNNCC 2 cut(s) 387, 688
BmrFI CCNGG 2 cut(s) 935, 1102
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 2 cut(s) 764, 1099
BmuI ACTGGG 1 cut(s) 999
BpiI GAAGAC 2 cut(s) 86, 474
BpuEI CTTGAG 1 cut(s) 140
BsaHI GRCGYC 2 cut(s) 147, 339
BsaJI CCNNGG 3 cut(s) 38, 334, 459
BsaXI ACNNNNNCTCC 6 cut(s) 455, 485, 491, 521, 643, 673
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 157, 160, 1426
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 3 cut(s) 513, 994, 1235
Bse3DI GCAATG 1 cut(s) 1371
BseBI CCWGG 2 cut(s) 935, 1102
BseDI CCNNGG 3 cut(s) 38, 334, 459
BseGI GGATG 7 cut(s) 253, 558, 790, 831, 1114, 1150, 1277
BseLI CCNNNNNNNGG 4 cut(s) 132, 157, 160, 1426
BseMI GCAATG 1 cut(s) 1371
BseMII CTCAG 5 cut(s) 85, 103, 340, 1153, 1175
BseNI ACTGG 3 cut(s) 513, 994, 1235
BsePI GCGCGC 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 3 cut(s) 359, 753, 1315
Bsh1236I CGCG 4 cut(s) 138, 166, 222, 574
Bsh1285I CGRYCG 1 cut(s) 764
BshFI GGCC 3 cut(s) 357, 447, 636
BsiEI CGRYCG 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 431
BsiSI CCGG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 4 cut(s) 132, 157, 160, 1426
BsmAI GTCTC 1 cut(s) 173
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 3 cut(s) 357, 447, 636
Bsp1286I GDGCHC 1 cut(s) 431
Bsp143I GATC 2 cut(s) 761, 1088
Bsp19I CCATGG 1 cut(s) 38
Bsp68I TCGCGA 1 cut(s) 574
BspACI CCGC 3 cut(s) 133, 161, 389
BspANI GGCC 3 cut(s) 357, 447, 636
BspCNI CTCAG 5 cut(s) 84, 102, 339, 1152, 1174
BspFNI CGCG 4 cut(s) 138, 166, 222, 574
BspLI GGNNCC 2 cut(s) 387, 688
BspQI GCTCTTC 1 cut(s) 322
BspTI CTTAAG 1 cut(s) 1067
BsrDI GCAATG 1 cut(s) 1371
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 3 cut(s) 513, 994, 1235
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 3 cut(s) 38, 334, 459
BssHII GCGCGC 1 cut(s) 136
BssMI GATC 2 cut(s) 761, 1088
BssNI GRCGYC 2 cut(s) 147, 339
BssSI CACGAG 1 cut(s) 52
BssT1I CCWWGG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 52
Bst2UI CCWGG 2 cut(s) 935, 1102
Bst4CI ACNGT 4 cut(s) 210, 229, 659, 1020
Bst6I CTCTTC 3 cut(s) 199, 322, 1001
BstACI GRCGYC 2 cut(s) 147, 339
BstAFI CTTAAG 1 cut(s) 1067
BstC8I GCNNGC 3 cut(s) 62, 138, 393
BstDEI CTNAG 6 cut(s) 71, 89, 326, 638, 1139, 1161
BstDSI CCRYGG 3 cut(s) 38, 334, 459
BstF5I GGATG 7 cut(s) 253, 558, 790, 831, 1114, 1150, 1277
BstFNI CGCG 4 cut(s) 138, 166, 222, 574
BstHHI GCGC 6 cut(s) 138, 140, 166, 372, 916, 933
BstKTI GATC 2 cut(s) 764, 1091
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 2 cut(s) 761, 1088
BstMCI CGRYCG 1 cut(s) 764
BstMWI GCNNNNNNNGC 3 cut(s) 146, 547, 1118
BstNI CCWGG 2 cut(s) 935, 1102
BstNSI RCATGY 1 cut(s) 395
BstSCI CCNGG 2 cut(s) 933, 1100
BstUI CGCG 4 cut(s) 138, 166, 222, 574
BstV1I GCAGC 3 cut(s) 359, 753, 1315
BstV2I GAAGAC 2 cut(s) 86, 474
BstXI CCANNNNNNTGG 1 cut(s) 941
BsuRI GGCC 3 cut(s) 357, 447, 636
BtgI CCRYGG 3 cut(s) 38, 334, 459
BtgZI GCGATG 2 cut(s) 456, 555
BtsCI GGATG 7 cut(s) 253, 558, 790, 831, 1114, 1150, 1277
BtsIMutI CAGTG 2 cut(s) 238, 520
BtuMI TCGCGA 1 cut(s) 574
Cac8I GCNNGC 3 cut(s) 62, 138, 393
CfoI GCGC 6 cut(s) 138, 140, 166, 372, 916, 933
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 2 cut(s) 385, 634
CseI GACGC 4 cut(s) 155, 155, 211, 328
Csp6I GTAC 4 cut(s) 23, 859, 882, 1205
CspCI CAANNNNNGTGG 2 cut(s) 1249, 1284
CviAII CATG 4 cut(s) 39, 392, 667, 822
CviQI GTAC 4 cut(s) 23, 859, 882, 1205
DdeI CTNAG 6 cut(s) 71, 89, 326, 638, 1139, 1161
DpnI GATC 2 cut(s) 763, 1090
DpnII GATC 2 cut(s) 761, 1088
DraI TTTAAA 1 cut(s) 1000
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 3 cut(s) 199, 322, 1001
EarI CTCTTC 3 cut(s) 199, 322, 1001
Eco130I CCWWGG 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 1307
EcoRII CCWGG 2 cut(s) 933, 1100
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 4 cut(s) 42, 395, 670, 825
FaqI GGGAC 1 cut(s) 371
FatI CATG 4 cut(s) 38, 391, 666, 821
FauI CCCGC 1 cut(s) 396
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 5 cut(s) 134, 162, 373, 767, 1304
FokI GGATG 7 cut(s) 240, 545, 797, 838, 1121, 1157, 1284
Fsp4HI GCNGC 5 cut(s) 134, 162, 373, 767, 1304
FspBI CTAG 1 cut(s) 248
GlaI GCGC 6 cut(s) 137, 139, 165, 371, 915, 932
GluI GCNGC 5 cut(s) 134, 162, 373, 767, 1304
HaeIII GGCC 3 cut(s) 357, 447, 636
HapII CCGG 1 cut(s) 419
HgaI GACGC 4 cut(s) 155, 155, 211, 328
HhaI GCGC 6 cut(s) 138, 140, 166, 372, 916, 933
Hin1I GRCGYC 2 cut(s) 147, 339
Hin1II CATG 4 cut(s) 42, 395, 670, 825
Hin6I GCGC 6 cut(s) 136, 138, 164, 370, 914, 931
HinP1I GCGC 6 cut(s) 136, 138, 164, 370, 914, 931
HincII GTYRAC 1 cut(s) 1158
HindII GTYRAC 1 cut(s) 1158
HindIII AAGCTT 1 cut(s) 529
HinfI GANTC 7 cut(s) 322, 470, 484, 617, 876, 1063, 1159
HpaII CCGG 1 cut(s) 419
HphI GGTGA 3 cut(s) 198, 217, 1259
Hpy166II GTNNAC 8 cut(s) 213, 463, 960, 1158, 1207, 1236, 1250, 1420
Hpy188I TCNGA 8 cut(s) 329, 433, 494, 616, 1076, 1093, 1164, 1351
Hpy188III TCNNGA 5 cut(s) 573, 759, 974, 1178, 1325
Hpy8I GTNNAC 8 cut(s) 213, 463, 960, 1158, 1207, 1236, 1250, 1420
Hpy99I CGWCG 3 cut(s) 344, 368, 385
HpyAV CCTTC 3 cut(s) 276, 824, 1099
HpyCH4III ACNGT 4 cut(s) 210, 229, 659, 1020
HpyCH4V TGCA 6 cut(s) 236, 817, 856, 1121, 1172, 1303
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 547, 1118
HpyF3I CTNAG 6 cut(s) 71, 89, 326, 638, 1139, 1161
Hsp92I GRCGYC 2 cut(s) 147, 339
Hsp92II CATG 4 cut(s) 42, 395, 670, 825
HspAI GCGC 6 cut(s) 136, 138, 164, 370, 914, 931
Kzo9I GATC 2 cut(s) 761, 1088
LguI GCTCTTC 1 cut(s) 322
LmnI GCTCC 3 cut(s) 268, 607, 692
Lsp1109I GCAGC 3 cut(s) 359, 753, 1315
LweI GCATC 2 cut(s) 764, 1099
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 223, 653
MalI GATC 2 cut(s) 763, 1090
MboI GATC 2 cut(s) 761, 1088
MhlI GDGCHC 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 447
MlyI GAGTC 2 cut(s) 464, 1153
Mox20I TGGCCA 1 cut(s) 447
MroXI GAANNNNTTC 2 cut(s) 1332, 1395
MscI TGGCCA 1 cut(s) 447
MseI TTAA 9 cut(s) 27, 524, 597, 644, 969, 999, 1068, 1185, 1379
MslI CAYNNNNRTG 3 cut(s) 406, 824, 1126
Msp20I TGGCCA 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 1067
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 935, 1102
MvaI CCWGG 2 cut(s) 935, 1102
MvnI CGCG 4 cut(s) 138, 166, 222, 574
MwoI GCNNNNNNNGC 3 cut(s) 146, 547, 1118
NcoI CCATGG 1 cut(s) 38
NdeII GATC 2 cut(s) 761, 1088
NlaIII CATG 4 cut(s) 42, 395, 670, 825
NlaIV GGNNCC 2 cut(s) 387, 688
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 2 cut(s) 223, 653
NruI TCGCGA 1 cut(s) 574
NspI RCATGY 1 cut(s) 395
PaeI GCATGC 1 cut(s) 395
PalAI GGCGCGCC 1 cut(s) 136
PauI GCGCGC 1 cut(s) 136
PciSI GCTCTTC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdmI GAANNNNTTC 2 cut(s) 1332, 1395
PfeI GAWTC 5 cut(s) 322, 484, 617, 876, 1063
PflFI GACNNNGTC 1 cut(s) 1415
PkrI GCNGC 5 cut(s) 135, 163, 374, 768, 1305
Ple19I CGATCG 1 cut(s) 764
PleI GAGTC 2 cut(s) 464, 1153
PpsI GAGTC 2 cut(s) 464, 1153
PshBI ATTAAT 1 cut(s) 969
Psp6I CCWGG 2 cut(s) 933, 1100
PspGI CCWGG 2 cut(s) 933, 1100
PspN4I GGNNCC 2 cut(s) 387, 688
PspPI GGNCC 2 cut(s) 385, 634
PsyI GACNNNGTC 1 cut(s) 1415
PteI GCGCGC 1 cut(s) 136
PvuI CGATCG 1 cut(s) 764
RruI TCGCGA 1 cut(s) 574
RsaI GTAC 4 cut(s) 24, 860, 883, 1206
RsaNI GTAC 4 cut(s) 23, 859, 882, 1205
RseI CAYNNNNRTG 3 cut(s) 406, 824, 1126
SapI GCTCTTC 1 cut(s) 322
SaqAI TTAA 9 cut(s) 27, 524, 597, 644, 969, 999, 1068, 1185, 1379
SatI GCNGC 5 cut(s) 134, 162, 373, 767, 1304
Sau3AI GATC 2 cut(s) 761, 1088
Sau96I GGNCC 2 cut(s) 385, 634
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 2 cut(s) 464, 1153
ScrFI CCNGG 2 cut(s) 935, 1102
SduI GDGCHC 1 cut(s) 431
SfaNI GCATC 2 cut(s) 764, 1099
SgsI GGCGCGCC 1 cut(s) 136
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 3 cut(s) 406, 824, 1126
SmlI CTYRAG 2 cut(s) 155, 1067
SmoI CTYRAG 2 cut(s) 155, 1067
SphI GCATGC 1 cut(s) 395
SsiI CCGC 3 cut(s) 133, 161, 389
SspI AATATT 1 cut(s) 1312
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 933, 1100
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 4 cut(s) 210, 229, 659, 1020
TaqI TCGA 4 cut(s) 296, 320, 482, 519
TaqII GACCGA 1 cut(s) 373
TatI WGTACW 1 cut(s) 858
TauI GCSGC 2 cut(s) 136, 164
TfiI GAWTC 5 cut(s) 322, 484, 617, 876, 1063
Tru1I TTAA 9 cut(s) 27, 524, 597, 644, 969, 999, 1068, 1185, 1379
Tru9I TTAA 9 cut(s) 27, 524, 597, 644, 969, 999, 1068, 1185, 1379
TscAI CASTG 2 cut(s) 238, 520
TseFI GTSAC 2 cut(s) 223, 653
TseI GCWGC 3 cut(s) 372, 766, 1303
Tsp45I GTSAC 2 cut(s) 223, 653
TspDTI ATGAA 3 cut(s) 547, 1019, 1161
TspGWI ACGGA 4 cut(s) 352, 698, 869, 977
TspRI CASTG 2 cut(s) 238, 520
Tth111I GACNNNGTC 1 cut(s) 1415
Vha464I CTTAAG 1 cut(s) 1067
VpaK11BI GGWCC 1 cut(s) 385
VspI ATTAAT 1 cut(s) 969
XapI RAATTY 5 cut(s) 553, 1042, 1148, 1329, 1392
XceI RCATGY 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 212
XmnI GAANNNNTTC 2 cut(s) 1332, 1395
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.