pycom01g18400

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004647
Physical Location & Seq
Reverse (-)
17410252 .. 17414481
4230 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1596 bp
ATGGAGCTAACTACAACTGAAACTGAGAGAGAGAGAGAGAGAGAGATGGAGGTGTCTCTTCTGTTGAGAATAATTTCTTCCATGGCTGTCGTGGGACTGGTTGGCTTCTTCCTCCACCTCTACAGCACTGTCTGGCTAAAATCCGAGAGGGTCAGAAGGAAGCTCCGACTGCAAGGCATCAAAGGCCCTGCACCTTCTTTTCTGTATGGGAATCTTCCCGAGATGCAGAAAATCCAACAACTGCAAGAGTTGAAGCCTCCAAACCTGCTGGCTGCTGAGTTTGTAGCCCATGACTACACCTCCACTCTCTTCCCATACTTCGAACACTGGAGAAAACAATACGGTCCAGTGTACACATATTCCACAGGAATGAGGCAACATTTGTATGTGAACAACCCAGAGATAGTGAAGGAGATGAACCAGTGTATGAATTTGGATTTAGGCAAGCCTTCTTATGTAAGCAAGAGACTTGCACCCATGCTTGGCAATGGCATTTTGAGGTCCAATGGCATTGTTTGGTCACAGCAAAGGAAGATTGTTGCCCCCGAGTTCTTCTCAGACAAAGTCAAGGGCATGGTGTGGCTGATGATGGAGTCTGGACAGACATTGCTTAGAAAATGGGAGGACTCCATTGAAGCACAAGGCGGTGTGAGAGCGGAGATTCAGGTGGATGAGGATTTGAGAGATTTCTCTGCAGATGTTATATCAAGAGCTTGTTTTGGAAGCTCTTACGTAAAGGGCAAAGAGATTTTCAAAAAACTTAGAAGTCTTCAAGAAGTGATATCCAAGCAAAACTTCCTCTTTAGTAATTTGGGTGGATTGAAGAAGACAAACGAGATAGGCTGTTTGGAGAGGGAGATTGAGTCTTTGATCTGGGAGGCAGTGGAAGAAAGAGAAGGCTCATGTGACCAAAAAGATTTGTTGCAGACAATACTAGAGGGAGCCATCAATGACCAAACCCTAGGGAAATGTTCATCCAAGCGCTTCATAGTTGACAACTGCAAGAACATATATTTTGCAGGCCATGAATCCACAGCTGTTGCTGCCTCTTGGTGTTTGATGCTTCTCGCTTTACATCCCGAGTGGCAAGCCCGAATCAGGACGGAGTTGGTTCAAGCTTGCCCGGATGGCCTGCCGGACGCAAACTCCCTCCCTCAGTTGAAAACGATGGCCATGGTGATTCAAGAAGTCATGCGTCTATATCCGCCAGCCGCATTTGTGTCGAGAGAGGCACTCGAAGACACTCAAGTGGGAAACATGACCGTTCCAAAAGGCGTATGCTTATGGACATTGATCCCAACATTGCACAGAGACACAGAAATTTGGGGACCAGATGCAAATGAGTTCAAACCAGAGAGGTTCACTGATGGAGTCTCAAAGGCATGCAAGTTTCCCCAAGCTTATATCCCATTCGGGTTGGGGCCTAGGACATGTTTGGGCAAAAACTTAGCCATGGTTGAACTGAAGGTTGTGCTCTCACTGGTTATCTCCAAGTTTTCTTTCTCTCTATCTCCAAAATACAAGCATTCCCCTGCCTACAAAATGATTGTGGGGCCAGGAAATGGTGTACATATTATTCTCCAGAAAATCTGTTGA

Protein Analysis

532

Amino Acids

60.16

Weight (kDa)

8.45

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 101 - 512 1.3e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AccB7I CCANNNNNTGG 1 cut(s) 1562
AccBSI CCGCTC 1 cut(s) 656
AciI CCGC 4 cut(s) 645, 656, 1205, 1212
AclWI GGATC 1 cut(s) 1288
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 2 cut(s) 430, 1320
AcuI CTGAAG 1 cut(s) 1484
AfaI GTAC 2 cut(s) 353, 1569
AfeI AGCGCT 1 cut(s) 983
AfiI CCNNNNNNNGG 3 cut(s) 482, 1098, 1562
AflIII ACRYGT 1 cut(s) 1430
AjnI CCWGG 1 cut(s) 1555
AjuI GAANNNNNNNTTGG 4 cut(s) 779, 811, 1484, 1516
AleI CACNNNNGTG 1 cut(s) 1247
AluBI AGCT 7 cut(s) 7, 163, 713, 726, 1037, 1118, 1400
AluI AGCT 7 cut(s) 7, 163, 713, 726, 1037, 1118, 1400
Alw21I GWGCWC 1 cut(s) 1476
Alw26I GTCTC 4 cut(s) 60, 460, 1305, 1378
AlwI GGATC 1 cut(s) 1288
Ama87I CYCGRG 3 cut(s) 218, 545, 1079
Aor51HI AGCGCT 1 cut(s) 983
AoxI GGCC 6 cut(s) 184, 1021, 1129, 1170, 1421, 1553
ApeKI GCWGC 2 cut(s) 272, 1043
ApoI RAATTY 2 cut(s) 430, 1320
Asp700I GAANNNNTTC 1 cut(s) 73
AspA2I CCTAGG 2 cut(s) 961, 1424
AspLEI GCGC 1 cut(s) 984
AspS9I GGNCC 6 cut(s) 185, 344, 501, 1328, 1421, 1553
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 1 cut(s) 1189
AsuII TTCGAA 1 cut(s) 321
AvaI CYCGRG 3 cut(s) 218, 545, 1079
AvaII GGWCC 3 cut(s) 344, 501, 1328
AvrII CCTAGG 2 cut(s) 961, 1424
BalI TGGCCA 1 cut(s) 1172
BbsI GAAGAC 3 cut(s) 761, 833, 1245
Bbv12I GWGCWC 1 cut(s) 1476
BbvI GCAGC 2 cut(s) 259, 1030
BccI CCATC 6 cut(s) 40, 583, 953, 1121, 1162, 1361
BcgI CGANNNNNNTGC 2 cut(s) 1203, 1237
BciT130I CCWGG 1 cut(s) 1557
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 4 cut(s) 60, 460, 1305, 1378
BfaI CTAG 3 cut(s) 935, 962, 1425
BfmI CTRYAG 2 cut(s) 121, 693
BfoI RGCGCY 1 cut(s) 985
BfuAI ACCTGC 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 1128
BisI GCNGC 3 cut(s) 273, 1044, 1212
BlnI CCTAGG 2 cut(s) 961, 1424
BlsI GCNGC 3 cut(s) 274, 1045, 1213
Bme1390I CCNGG 2 cut(s) 1124, 1557
Bme18I GGWCC 3 cut(s) 344, 501, 1328
BmeT110I CYCGRG 3 cut(s) 218, 545, 1079
BmgT120I GGNCC 6 cut(s) 185, 344, 501, 1328, 1421, 1553
BmiI GGNNCC 4 cut(s) 943, 1329, 1422, 1554
BmrFI CCNGG 2 cut(s) 1124, 1557
BmsI GCATC 4 cut(s) 186, 213, 1050, 1324
BpiI GAAGAC 3 cut(s) 761, 833, 1245
BplI GAGNNNNNCTC 4 cut(s) 539, 571, 1218, 1250
BpmI CTGGAG 2 cut(s) 349, 1565
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 1230
BpuMI CCSGG 1 cut(s) 1124
BsaAI YACGTR 1 cut(s) 733
BsaJI CCNNGG 5 cut(s) 81, 961, 1173, 1424, 1452
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 848, 878, 1130, 1160
Bsc4I CCNNNNNNNGG 3 cut(s) 482, 1098, 1562
Bse1I ACTGG 5 cut(s) 102, 332, 347, 421, 1485
Bse3DI GCAATG 3 cut(s) 493, 605, 1301
BseBI CCWGG 1 cut(s) 1557
BseDI CCNNGG 5 cut(s) 81, 961, 1173, 1424, 1452
BseGI GGATG 4 cut(s) 676, 974, 1075, 1132
BseLI CCNNNNNNNGG 3 cut(s) 482, 1098, 1562
BseMI GCAATG 3 cut(s) 493, 605, 1301
BseMII CTCAG 4 cut(s) 15, 267, 570, 1169
BseNI ACTGG 5 cut(s) 102, 332, 347, 421, 1485
BseXI GCAGC 2 cut(s) 259, 1030
BsgI GTGCAG 1 cut(s) 174
BshFI GGCC 6 cut(s) 186, 1023, 1131, 1172, 1423, 1555
BsiHKAI GWGCWC 1 cut(s) 1476
BsiHKCI CYCGRG 3 cut(s) 218, 545, 1079
BsiSI CCGG 2 cut(s) 1124, 1136
BslFI GGGAC 2 cut(s) 108, 1341
BslI CCNNNNNNNGG 3 cut(s) 482, 1098, 1562
BsmAI GTCTC 4 cut(s) 60, 460, 1305, 1378
BsmFI GGGAC 2 cut(s) 108, 1341
BsmI GAATGC 1 cut(s) 1525
BsnI GGCC 6 cut(s) 186, 1023, 1131, 1172, 1423, 1555
BsoBI CYCGRG 3 cut(s) 218, 545, 1079
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 1476
Bsp1407I TGTACA 2 cut(s) 351, 1567
Bsp143I GATC 2 cut(s) 870, 1293
Bsp19I CCATGG 3 cut(s) 81, 1173, 1452
BspACI CCGC 4 cut(s) 645, 656, 1205, 1212
BspANI GGCC 6 cut(s) 186, 1023, 1131, 1172, 1423, 1555
BspCNI CTCAG 4 cut(s) 16, 268, 569, 1168
BspLI GGNNCC 4 cut(s) 943, 1329, 1422, 1554
BspMAI CTGCAG 1 cut(s) 697
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 1 cut(s) 1288
BspT104I TTCGAA 1 cut(s) 321
BsrBI CCGCTC 1 cut(s) 656
BsrDI GCAATG 3 cut(s) 493, 605, 1301
BsrGI TGTACA 2 cut(s) 351, 1567
BsrI ACTGG 5 cut(s) 102, 332, 347, 421, 1485
BssECI CCNNGG 5 cut(s) 81, 961, 1173, 1424, 1452
BssMI GATC 2 cut(s) 870, 1293
BssT1I CCWWGG 5 cut(s) 81, 961, 1173, 1424, 1452
Bst2UI CCWGG 1 cut(s) 1557
Bst4CI ACNGT 3 cut(s) 130, 344, 1264
Bst6I CTCTTC 2 cut(s) 63, 314
BstAUI TGTACA 2 cut(s) 351, 1567
BstBAI YACGTR 1 cut(s) 733
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 8 cut(s) 270, 446, 1021, 1089, 1120, 1133, 1209, 1384
BstDEI CTNAG 7 cut(s) 24, 276, 556, 611, 761, 1155, 1447
BstDSI CCRYGG 3 cut(s) 81, 1173, 1452
BstF5I GGATG 4 cut(s) 676, 974, 1075, 1132
BstH2I RGCGCY 1 cut(s) 985
BstHHI GCGC 1 cut(s) 984
BstKTI GATC 2 cut(s) 873, 1296
BstMAI GTCTC 4 cut(s) 60, 460, 1305, 1378
BstMBI GATC 2 cut(s) 870, 1293
BstMWI GCNNNNNNNGC 4 cut(s) 169, 183, 1043, 1128
BstNI CCWGG 1 cut(s) 1557
BstNSI RCATGY 2 cut(s) 1386, 1434
BstSCI CCNGG 2 cut(s) 1122, 1555
BstSFI CTRYAG 2 cut(s) 121, 693
BstSNI TACGTA 1 cut(s) 733
BstV1I GCAGC 2 cut(s) 259, 1030
BstV2I GAAGAC 3 cut(s) 761, 833, 1245
BsuRI GGCC 6 cut(s) 186, 1023, 1131, 1172, 1423, 1555
BtgI CCRYGG 3 cut(s) 81, 1173, 1452
BtsCI GGATG 4 cut(s) 676, 974, 1075, 1132
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 7 cut(s) 126, 325, 354, 428, 888, 1362, 1478
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 8 cut(s) 270, 446, 1021, 1089, 1120, 1133, 1209, 1384
CfoI GCGC 1 cut(s) 984
Cfr13I GGNCC 6 cut(s) 185, 344, 501, 1328, 1421, 1553
CseI GACGC 2 cut(s) 1148, 1184
Csp6I GTAC 2 cut(s) 352, 1568
CspCI CAANNNNNGTGG 2 cut(s) 1021, 1056
CviQI GTAC 2 cut(s) 352, 1568
DdeI CTNAG 7 cut(s) 24, 276, 556, 611, 761, 1155, 1447
DpnI GATC 2 cut(s) 872, 1295
DpnII GATC 2 cut(s) 870, 1293
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 2 cut(s) 63, 314
EarI CTCTTC 2 cut(s) 63, 314
EciI GGCGGA 1 cut(s) 1194
Eco105I TACGTA 1 cut(s) 733
Eco130I CCWWGG 5 cut(s) 81, 961, 1173, 1424, 1452
Eco32I GATATC 1 cut(s) 783
Eco47I GGWCC 3 cut(s) 344, 501, 1328
Eco47III AGCGCT 1 cut(s) 983
Eco57I CTGAAG 1 cut(s) 1484
Eco88I CYCGRG 3 cut(s) 218, 545, 1079
EcoO109I RGGNCCY 2 cut(s) 185, 1421
EcoRII CCWGG 1 cut(s) 1555
EcoRV GATATC 1 cut(s) 783
EcoT14I CCWWGG 5 cut(s) 81, 961, 1173, 1424, 1452
ErhI CCWWGG 5 cut(s) 81, 961, 1173, 1424, 1452
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FaqI GGGAC 2 cut(s) 108, 1341
Fnu4HI GCNGC 3 cut(s) 273, 1044, 1212
FokI GGATG 4 cut(s) 683, 961, 1062, 1139
Fsp4HI GCNGC 3 cut(s) 273, 1044, 1212
FspBI CTAG 3 cut(s) 935, 962, 1425
GlaI GCGC 1 cut(s) 983
GluI GCNGC 3 cut(s) 273, 1044, 1212
GsuI CTGGAG 2 cut(s) 349, 1565
HaeII RGCGCY 1 cut(s) 985
HaeIII GGCC 6 cut(s) 186, 1023, 1131, 1172, 1423, 1555
HapII CCGG 2 cut(s) 1124, 1136
HgaI GACGC 2 cut(s) 1148, 1184
HhaI GCGC 1 cut(s) 984
Hin6I GCGC 1 cut(s) 982
HinP1I GCGC 1 cut(s) 982
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HindIII AAGCTT 2 cut(s) 1116, 1398
HinfI GANTC 9 cut(s) 211, 593, 626, 661, 863, 1028, 1095, 1180, 1371
HpaII CCGG 2 cut(s) 1124, 1136
HphI GGTGA 1 cut(s) 1189
Hpy166II GTNNAC 6 cut(s) 352, 354, 391, 994, 1362, 1568
Hpy188I TCNGA 4 cut(s) 145, 155, 167, 559
Hpy188III TCNNGA 9 cut(s) 218, 597, 708, 773, 1079, 1099, 1184, 1224, 1582
Hpy8I GTNNAC 6 cut(s) 352, 354, 391, 994, 1362, 1568
HpyAV CCTTC 6 cut(s) 150, 204, 403, 459, 890, 1459
HpyCH4III ACNGT 3 cut(s) 130, 344, 1264
HpyCH4IV ACGT 1 cut(s) 732
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 183, 1043, 1128
HpyF3I CTNAG 7 cut(s) 24, 276, 556, 611, 761, 1155, 1447
HpySE526I ACGT 1 cut(s) 732
HspAI GCGC 1 cut(s) 982
Kzo9I GATC 2 cut(s) 870, 1293
LmnI GCTCC 3 cut(s) 4, 168, 941
Lsp1109I GCAGC 2 cut(s) 259, 1030
LweI GCATC 4 cut(s) 186, 213, 1050, 1324
MaeI CTAG 3 cut(s) 935, 962, 1425
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 2 cut(s) 519, 905
MalI GATC 2 cut(s) 872, 1295
MbiI CCGCTC 1 cut(s) 656
MboI GATC 2 cut(s) 870, 1293
MhlI GDGCHC 1 cut(s) 1476
MlsI TGGCCA 1 cut(s) 1172
MluCI AATT 4 cut(s) 72, 430, 808, 1320
MluNI TGGCCA 1 cut(s) 1172
MlyI GAGTC 4 cut(s) 602, 620, 872, 1380
MmeI TCCRAC 2 cut(s) 190, 259
Mox20I TGGCCA 1 cut(s) 1172
MroXI GAANNNNTTC 1 cut(s) 73
MscI TGGCCA 1 cut(s) 1172
MslI CAYNNNNRTG 3 cut(s) 368, 384, 1247
Msp20I TGGCCA 1 cut(s) 1172
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 2 cut(s) 1124, 1136
MspR9I CCNGG 2 cut(s) 1124, 1557
Mva1269I GAATGC 1 cut(s) 1525
MvaI CCWGG 1 cut(s) 1557
MwoI GCNNNNNNNGC 4 cut(s) 169, 183, 1043, 1128
NciI CCSGG 1 cut(s) 1124
NcoI CCATGG 3 cut(s) 81, 1173, 1452
NdeII GATC 2 cut(s) 870, 1293
NlaIV GGNNCC 4 cut(s) 943, 1329, 1422, 1554
NmuCI GTSAC 2 cut(s) 519, 905
NspI RCATGY 2 cut(s) 1386, 1434
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 1247
PaeI GCATGC 1 cut(s) 1386
PciI ACATGT 1 cut(s) 1430
PctI GAATGC 1 cut(s) 1525
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 5 cut(s) 211, 661, 1028, 1095, 1180
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 1562
PkrI GCNGC 3 cut(s) 274, 1045, 1213
PleI GAGTC 4 cut(s) 601, 620, 871, 1379
PpsI GAGTC 4 cut(s) 601, 620, 871, 1379
Ppu21I YACGTR 1 cut(s) 733
PscI ACATGT 1 cut(s) 1430
Psp6I CCWGG 1 cut(s) 1555
PspGI CCWGG 1 cut(s) 1555
PspN4I GGNNCC 4 cut(s) 943, 1329, 1422, 1554
PspPI GGNCC 6 cut(s) 185, 344, 501, 1328, 1421, 1553
PstI CTGCAG 1 cut(s) 697
PsyI GACNNNGTC 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 2 cut(s) 353, 1569
RsaNI GTAC 2 cut(s) 352, 1568
RseI CAYNNNNRTG 3 cut(s) 368, 384, 1247
SatI GCNGC 3 cut(s) 273, 1044, 1212
Sau3AI GATC 2 cut(s) 870, 1293
Sau96I GGNCC 6 cut(s) 185, 344, 501, 1328, 1421, 1553
SchI GAGTC 4 cut(s) 602, 620, 872, 1380
ScrFI CCNGG 2 cut(s) 1124, 1557
SduI GDGCHC 1 cut(s) 1476
SfaNI GCATC 4 cut(s) 186, 213, 1050, 1324
SfcI CTRYAG 2 cut(s) 121, 693
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 3 cut(s) 344, 501, 1328
SmiMI CAYNNNNRTG 3 cut(s) 368, 384, 1247
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
SnaBI TACGTA 1 cut(s) 733
SphI GCATGC 1 cut(s) 1386
Sse9I AATT 4 cut(s) 72, 430, 808, 1320
SsiI CCGC 4 cut(s) 645, 656, 1205, 1212
SspMI CTAG 3 cut(s) 935, 962, 1425
StyD4I CCNGG 2 cut(s) 1122, 1555
StyI CCWWGG 5 cut(s) 81, 961, 1173, 1424, 1452
TaaI ACNGT 3 cut(s) 130, 344, 1264
TaiI ACGT 1 cut(s) 735
TaqI TCGA 3 cut(s) 321, 1223, 1236
TasI AATT 4 cut(s) 72, 430, 808, 1320
TatI WGTACW 2 cut(s) 351, 1567
TauI GCSGC 1 cut(s) 1214
TfiI GAWTC 5 cut(s) 211, 661, 1028, 1095, 1180
TscAI CASTG 7 cut(s) 133, 332, 354, 428, 888, 1369, 1485
TseFI GTSAC 2 cut(s) 519, 905
TseI GCWGC 2 cut(s) 272, 1043
Tsp45I GTSAC 2 cut(s) 519, 905
TspDTI ATGAA 5 cut(s) 431, 443, 963, 976, 1041
TspGWI ACGGA 1 cut(s) 1118
TspRI CASTG 7 cut(s) 133, 332, 354, 428, 888, 1369, 1485
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 1562
VpaK11BI GGWCC 3 cut(s) 344, 501, 1328
XapI RAATTY 2 cut(s) 430, 1320
XceI RCATGY 2 cut(s) 1386, 1434
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmaJI CCTAGG 2 cut(s) 961, 1424
XmnI GAANNNNTTC 1 cut(s) 73
XspI CTAG 3 cut(s) 935, 962, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.