pycom01g18780

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008946
Physical Location & Seq
Reverse (-)
17638902 .. 17643452
4551 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1614 bp
ATGGAATCCACCATTTCATCGCCTATCTCCTCCACCAAGTCCTCGCCCCTGTTGCATTCGGGTTCCCATTCACTCGGCTCCAGATCCCAGTCTTTTCCGTCGCTTCCGTTGGTTCAAACACTCCGCTATCCCAGCTTCAAATCCAGAAGCTTCCTCGGATTTCCGTTCCGTTCGCGCCAAAACCAGCCTATCCGATCTCAGAGCCCGAATCATCAGCCGGCTAACGACGGCTTCGTCCTCGAAGACGTTCCTCACTTGACTAATTTTCTCCCTAATTTGCCGTCATATCCAAATCCATTGAAACAGAGCCAAGCTTATGCAGTTGTCAAGCAAACTTTTGTGAGCCCTGACGATGTGGTTGCGCAGAAAGTTGTTGTCCAAAAGGAAGGTCCTAGAGGGGTGCATTTCCGACGTGCAGGACCCCGGGAAAAGGTTTATTTCAAGTCGGATGAAGTTCGTGCTTGCATAGTCACTTGTGGGGGTTTATGCCCAGGGATCAATACTGTGATTCGGGAAATAGTATGCGGCTTGAACTACATGTATGGTGTTGAAGATATACTTGGAATTGAGGGAGGATATAGGGGATTTTATTCTAAAAATACATTGAGGTTGACACCAAAAGTTGTCAATGATATTCATAAGCGCGGTGGCACCTTTCTTCGAACTTCACGAGGTGGCCACGATACGAACAAGATAGTTGATAACATACAGGACCGAGGAATAAATCAGGTATACATAATTGGAGGCGATGGCACTCAAAGAGGAGCGGATCTTATATACAAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGATCCCTAAGACAATTGATAATGATATTGCTGTGATAGACAAATCTTTTGGTTTTGATACTGCTGTTGAAGAAGCACAGAGAGCTATTAATGCTGCACATGTGGAGGTTGAAAGTGTGGAAAATGGAGTTGGAATTGTCAAACTCATGGGCAGATACAGTGGTTTCATTGCTCTACATGCAACTTTGGCAAGTCGAGATGTGGATTGCTGCTTGATTCCAGAATCTCCTTTCTATTTGGAAGGCCGGGGCGGGCTTTTTGAATTTGTCGAAGAGCGGCTTAAAGAAAATGGACACGTGGTTATTGTGTTAGCAGAAGGAGCAGGGCAGGAATATATTGCAGGGGAGATGAATGCTGTTGATGTGAAAGATGCATCAGGAAATAAGCTACTCCTCGATGTTGGTCTATGGTTAACCCAAAAGATTAAGGATCATTTTACAAAAGTTCAGAAGATGGCGATAAATATGAAATATATAGATCCCACCTATATGATTCGAGCTATCCCAAGTAATGCATCGGACAATATATACTGTACTCTTCTTGCTCAGAGTGCTGTTCATGGAGCCATGGCAGGATTCAGTGGTTTCACAGTCGGGCCTGTAAACAGTAGACATGCTTATATTCCAATTGCTCGTGTGACAGAGACGCAAAACACAGTAAAGTTGACAGATCGGATGTGGGCTAGACTTCTTGCATCAACGAACCAGCCTAGTTTCTTGGAATACAGTGAGGGGCAAGGAAAAGCTGAAGAGGATGTTGATGTAATAAACAACAACATGAGAGCTACTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

538

Amino Acids

59.0

Weight (kDa)

8.05

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 154 - 460 6.4e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1612
AasI GACNNNNNNGTC 1 cut(s) 233
Acc16I TGCGCA 1 cut(s) 363
AccB1I GGYRCC 1 cut(s) 650
AccBSI CCGCTC 2 cut(s) 767, 1096
AccI GTMKAC 2 cut(s) 732, 1429
AccII CGCG 2 cut(s) 175, 645
AciI CCGC 6 cut(s) 124, 525, 645, 767, 1071, 1096
AclWI GGATC 7 cut(s) 78, 503, 777, 817, 830, 1257, 1292
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 1 cut(s) 1082
AcuI CTGAAG 1 cut(s) 1587
AcvI CACGTG 1 cut(s) 1117
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 1 cut(s) 1354
AfiI CCNNNNNNNGG 1 cut(s) 430
AflIII ACRYGT 3 cut(s) 537, 919, 1114
AjiI CACGTC 1 cut(s) 413
AjnI CCWGG 1 cut(s) 490
AjuI GAANNNNNNNTTGG 4 cut(s) 543, 575, 933, 965
AluBI AGCT 8 cut(s) 135, 150, 314, 905, 1207, 1319, 1565, 1604
AluI AGCT 8 cut(s) 135, 150, 314, 905, 1207, 1319, 1565, 1604
Alw26I GTCTC 1 cut(s) 1457
AlwI GGATC 7 cut(s) 78, 503, 777, 817, 830, 1257, 1292
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 3 cut(s) 676, 1063, 1415
ApeKI GCWGC 2 cut(s) 914, 1029
ApoI RAATTY 1 cut(s) 1082
ArsI GACNNNNNNTTYG 4 cut(s) 373, 405, 851, 883
AseI ATTAAT 1 cut(s) 909
Asp700I GAANNNNTTC 1 cut(s) 246
AspLEI GCGC 3 cut(s) 177, 364, 645
AspS9I GGNCC 4 cut(s) 389, 419, 712, 1415
AsuC2I CCSGG 3 cut(s) 424, 425, 1067
AsuII TTCGAA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 3 cut(s) 389, 419, 712
BaeI ACNNNNGTAYC 4 cut(s) 870, 903, 967, 1000
BalI TGGCCA 1 cut(s) 678
BamHI GGATCC 1 cut(s) 822
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 2 cut(s) 206, 347
BauI CACGAG 2 cut(s) 669, 1452
BbrPI CACGTG 1 cut(s) 1117
BbsI GAAGAC 2 cut(s) 249, 794
BbvI GCAGC 2 cut(s) 901, 1016
BccI CCATC 2 cut(s) 743, 1267
BceAI ACGGC 2 cut(s) 244, 265
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 1 cut(s) 492
BcnI CCSGG 3 cut(s) 424, 425, 1067
BcoDI GTCTC 1 cut(s) 1457
BfaI CTAG 3 cut(s) 393, 1503, 1530
BisI GCNGC 4 cut(s) 526, 915, 1030, 1097
BlsI GCNGC 4 cut(s) 527, 916, 1031, 1098
Bme1390I CCNGG 4 cut(s) 424, 425, 492, 1067
Bme18I GGWCC 3 cut(s) 389, 419, 712
BmeT110I CYCGRG 1 cut(s) 423
BmgBI CACGTC 1 cut(s) 413
BmgT120I GGNCC 4 cut(s) 389, 419, 712, 1415
BmiI GGNNCC 6 cut(s) 64, 79, 421, 652, 824, 1384
BmrFI CCNGG 4 cut(s) 424, 425, 492, 1067
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 4 cut(s) 1180, 1202, 1343, 1523
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 2 cut(s) 249, 794
BpmI CTGGAG 1 cut(s) 64
Bpu14I TTCGAA 1 cut(s) 661
BpuMI CCSGG 3 cut(s) 424, 425, 1067
BsaAI YACGTR 1 cut(s) 1117
BsaJI CCNNGG 8 cut(s) 154, 422, 423, 490, 491, 715, 1066, 1386
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 987
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 8 cut(s) 154, 422, 423, 490, 491, 715, 1066, 1386
BseGI GGATG 3 cut(s) 454, 1500, 1579
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 2 cut(s) 212, 1379
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 3 cut(s) 19, 777, 1202
BseXI GCAGC 2 cut(s) 901, 1016
BseYI CCCAGC 2 cut(s) 131, 818
BsgI GTGCAG 2 cut(s) 435, 900
Bsh1236I CGCG 2 cut(s) 175, 645
BshFI GGCC 3 cut(s) 678, 1065, 1417
BshNI GGYRCC 1 cut(s) 650
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 218, 424, 1066
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 1457
BsmBI CGTCTC 1 cut(s) 1457
BsmI GAATGC 2 cut(s) 55, 1177
BsnI GGCC 3 cut(s) 678, 1065, 1417
BsoBI CYCGRG 1 cut(s) 423
Bsp119I TTCGAA 1 cut(s) 661
Bsp1286I GDGCHC 2 cut(s) 206, 347
Bsp143I GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
Bsp19I CCATGG 1 cut(s) 1386
BspACI CCGC 6 cut(s) 124, 525, 645, 767, 1071, 1096
BspANI GGCC 3 cut(s) 678, 1065, 1417
BspCNI CTCAG 2 cut(s) 211, 1378
BspFNI CGCG 2 cut(s) 175, 645
BspLI GGNNCC 6 cut(s) 64, 79, 421, 652, 824, 1384
BspPI GGATC 7 cut(s) 78, 503, 777, 817, 830, 1257, 1292
BspQI GCTCTTC 1 cut(s) 1086
BspT104I TTCGAA 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 650
BsrBI CCGCTC 2 cut(s) 767, 1096
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 8 cut(s) 154, 422, 423, 490, 491, 715, 1066, 1386
BssMI GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
BssNAI GTATAC 1 cut(s) 733
BssSI CACGAG 2 cut(s) 669, 1452
BssT1I CCWWGG 1 cut(s) 1386
Bst1107I GTATAC 1 cut(s) 733
Bst2BI CACGAG 2 cut(s) 669, 1452
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 7 cut(s) 505, 980, 1352, 1411, 1427, 1477, 1547
Bst6I CTCTTC 3 cut(s) 1086, 1362, 1563
BstBAI YACGTR 1 cut(s) 1117
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 3 cut(s) 219, 463, 1073
BstDEI CTNAG 3 cut(s) 198, 828, 1365
BstDSI CCRYGG 1 cut(s) 1386
BstF5I GGATG 3 cut(s) 454, 1500, 1579
BstFNI CGCG 2 cut(s) 175, 645
BstHHI GCGC 3 cut(s) 177, 364, 645
BstKTI GATC 8 cut(s) 86, 197, 498, 772, 825, 1252, 1300, 1492
BstMAI GTCTC 1 cut(s) 1457
BstMBI GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
BstMWI GCNNNNNNNGC 8 cut(s) 52, 132, 902, 911, 998, 1007, 1139, 1370
BstNI CCWGG 1 cut(s) 492
BstNSI RCATGY 4 cut(s) 541, 923, 1001, 1436
BstSCI CCNGG 4 cut(s) 422, 423, 490, 1065
BstUI CGCG 2 cut(s) 175, 645
BstV1I GCAGC 2 cut(s) 901, 1016
BstV2I GAAGAC 2 cut(s) 249, 794
BstX2I RGATCY 4 cut(s) 83, 769, 822, 1297
BstYI RGATCY 4 cut(s) 83, 769, 822, 1297
BstZ17I GTATAC 1 cut(s) 733
BsuRI GGCC 3 cut(s) 678, 1065, 1417
BtgI CCRYGG 1 cut(s) 1386
BtgZI GCGATG 2 cut(s) 3, 762
BtrI CACGTC 1 cut(s) 413
BtsCI GGATG 3 cut(s) 454, 1500, 1579
BtsIMutI CAGTG 3 cut(s) 985, 1405, 1552
Cac8I GCNNGC 3 cut(s) 219, 463, 1073
CaiI CAGNNNCTG 1 cut(s) 818
CfoI GCGC 3 cut(s) 177, 364, 645
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 4 cut(s) 389, 419, 712, 1415
Cfr9I CCCGGG 1 cut(s) 423
CseI GACGC 1 cut(s) 1474
Csp6I GTAC 1 cut(s) 1353
CviAII CATG 8 cut(s) 538, 920, 967, 998, 1379, 1387, 1433, 1597
CviQI GTAC 1 cut(s) 1353
DdeI CTNAG 3 cut(s) 198, 828, 1365
DpnI GATC 8 cut(s) 85, 196, 497, 771, 824, 1251, 1299, 1491
DpnII GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
DraIII CACNNNGTG 1 cut(s) 674
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 3 cut(s) 1086, 1362, 1563
EarI CTCTTC 3 cut(s) 1086, 1362, 1563
Eco130I CCWWGG 1 cut(s) 1386
Eco24I GRGCYC 2 cut(s) 206, 347
Eco47I GGWCC 3 cut(s) 389, 419, 712
Eco57I CTGAAG 1 cut(s) 1587
Eco72I CACGTG 1 cut(s) 1117
Eco88I CYCGRG 1 cut(s) 423
EcoO109I RGGNCCY 2 cut(s) 389, 419
EcoRII CCWGG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 1386
EcoT22I ATGCAT 2 cut(s) 1195, 1336
EcoT38I GRGCYC 2 cut(s) 206, 347
ErhI CCWWGG 1 cut(s) 1386
Esp3I CGTCTC 1 cut(s) 1457
FaeI CATG 8 cut(s) 541, 923, 970, 1001, 1382, 1390, 1436, 1600
FalI AAGNNNNNCTT 4 cut(s) 543, 575, 1083, 1115
FatI CATG 8 cut(s) 537, 919, 966, 997, 1378, 1386, 1432, 1596
FauI CCCGC 1 cut(s) 1064
FblI GTMKAC 2 cut(s) 732, 1429
Fnu4HI GCNGC 4 cut(s) 526, 915, 1030, 1097
FokI GGATG 3 cut(s) 461, 1507, 1586
FriOI GRGCYC 2 cut(s) 206, 347
Fsp4HI GCNGC 4 cut(s) 526, 915, 1030, 1097
FspBI CTAG 3 cut(s) 393, 1503, 1530
FspI TGCGCA 1 cut(s) 363
GlaI GCGC 3 cut(s) 176, 363, 644
GluI GCNGC 4 cut(s) 526, 915, 1030, 1097
GsaI CCCAGC 2 cut(s) 135, 822
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 3 cut(s) 678, 1065, 1417
HapII CCGG 3 cut(s) 218, 424, 1066
HgaI GACGC 1 cut(s) 1474
HhaI GCGC 3 cut(s) 177, 364, 645
Hin1II CATG 8 cut(s) 541, 923, 970, 1001, 1382, 1390, 1436, 1600
Hin6I GCGC 3 cut(s) 175, 362, 643
HinP1I GCGC 3 cut(s) 175, 362, 643
HincII GTYRAC 3 cut(s) 612, 1233, 1485
HindII GTYRAC 3 cut(s) 612, 1233, 1485
HindIII AAGCTT 2 cut(s) 148, 312
HinfI GANTC 7 cut(s) 5, 208, 508, 1036, 1043, 1312, 1395
HpaI GTTAAC 1 cut(s) 1233
HpaII CCGG 3 cut(s) 218, 424, 1066
Hpy166II GTNNAC 6 cut(s) 612, 733, 1233, 1423, 1430, 1485
Hpy188I TCNGA 9 cut(s) 158, 194, 201, 410, 448, 1269, 1339, 1368, 1494
Hpy188III TCNNGA 7 cut(s) 81, 144, 512, 669, 1016, 1040, 1197
Hpy8I GTNNAC 6 cut(s) 612, 733, 1233, 1423, 1430, 1485
Hpy99I CGWCG 3 cut(s) 103, 230, 414
HpyAV CCTTC 3 cut(s) 380, 1055, 1130
HpyCH4III ACNGT 7 cut(s) 505, 980, 1352, 1411, 1427, 1477, 1547
HpyCH4IV ACGT 4 cut(s) 246, 412, 796, 1116
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 132, 902, 911, 998, 1007, 1139, 1370
HpyF3I CTNAG 3 cut(s) 198, 828, 1365
HpySE526I ACGT 4 cut(s) 246, 412, 796, 1116
Hsp92II CATG 8 cut(s) 541, 923, 970, 1001, 1382, 1390, 1436, 1600
HspAI GCGC 3 cut(s) 175, 362, 643
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
KspAI GTTAAC 1 cut(s) 1233
Kzo9I GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 4 cut(s) 83, 764, 1139, 1382
Lsp1109I GCAGC 2 cut(s) 901, 1016
LweI GCATC 4 cut(s) 1180, 1202, 1343, 1523
MaeI CTAG 3 cut(s) 393, 1503, 1530
MaeII ACGT 4 cut(s) 246, 412, 796, 1116
MaeIII GTNAC 2 cut(s) 469, 1456
MalI GATC 8 cut(s) 85, 196, 497, 771, 824, 1251, 1299, 1491
MbiI CCGCTC 2 cut(s) 767, 1096
MboI GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
MboII GAAGA 9 cut(s) 254, 563, 650, 794, 902, 1103, 1282, 1349, 1580
MfeI CAATTG 2 cut(s) 834, 1446
MflI RGATCY 4 cut(s) 83, 769, 822, 1297
MhlI GDGCHC 2 cut(s) 206, 347
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 8 cut(s) 262, 274, 564, 738, 834, 954, 1082, 1446
MluNI TGGCCA 1 cut(s) 678
MmeI TCCRAC 3 cut(s) 426, 433, 931
Mox20I TGGCCA 1 cut(s) 678
Mph1103I ATGCAT 2 cut(s) 1195, 1336
MroNI GCCGGC 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 246
MscI TGGCCA 1 cut(s) 678
MseI TTAA 4 cut(s) 909, 1101, 1232, 1245
MslI CAYNNNNRTG 1 cut(s) 1307
Msp20I TGGCCA 1 cut(s) 678
MspI CCGG 3 cut(s) 218, 424, 1066
MspR9I CCNGG 4 cut(s) 424, 425, 492, 1067
MunI CAATTG 2 cut(s) 834, 1446
Mva1269I GAATGC 2 cut(s) 55, 1177
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 2 cut(s) 175, 645
MwoI GCNNNNNNNGC 8 cut(s) 52, 132, 902, 911, 998, 1007, 1139, 1370
NaeI GCCGGC 1 cut(s) 219
NciI CCSGG 3 cut(s) 424, 425, 1067
NcoI CCATGG 1 cut(s) 1386
NdeII GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 8 cut(s) 541, 923, 970, 1001, 1382, 1390, 1436, 1600
NlaIV GGNNCC 6 cut(s) 64, 79, 421, 652, 824, 1384
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 469, 1456
NsbI TGCGCA 1 cut(s) 363
NsiI ATGCAT 2 cut(s) 1195, 1336
NspI RCATGY 4 cut(s) 541, 923, 1001, 1436
NspV TTCGAA 1 cut(s) 661
PasI CCCWGGG 1 cut(s) 491
PciI ACATGT 2 cut(s) 537, 919
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 667
PctI GAATGC 2 cut(s) 55, 1177
PdiI GCCGGC 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 7 cut(s) 5, 208, 508, 1036, 1043, 1312, 1395
PkrI GCNGC 4 cut(s) 527, 916, 1031, 1098
PmaCI CACGTG 1 cut(s) 1117
PmlI CACGTG 1 cut(s) 1117
Ppu21I YACGTR 1 cut(s) 1117
PpuMI RGGWCCY 2 cut(s) 389, 419
PscI ACATGT 2 cut(s) 537, 919
PshBI ATTAAT 1 cut(s) 909
PsiI TTATAA 1 cut(s) 1612
Psp5II RGGWCCY 2 cut(s) 389, 419
Psp6I CCWGG 1 cut(s) 490
PspCI CACGTG 1 cut(s) 1117
PspFI CCCAGC 2 cut(s) 131, 818
PspGI CCWGG 1 cut(s) 490
PspN4I GGNNCC 6 cut(s) 64, 79, 421, 652, 824, 1384
PspPI GGNCC 4 cut(s) 389, 419, 712, 1415
PspPPI RGGWCCY 2 cut(s) 389, 419
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 4 cut(s) 83, 769, 822, 1297
RsaI GTAC 1 cut(s) 1354
RsaNI GTAC 1 cut(s) 1353
RseI CAYNNNNRTG 1 cut(s) 1307
SapI GCTCTTC 1 cut(s) 1086
SaqAI TTAA 4 cut(s) 909, 1101, 1232, 1245
SatI GCNGC 4 cut(s) 526, 915, 1030, 1097
Sau3AI GATC 8 cut(s) 83, 194, 495, 769, 822, 1249, 1297, 1489
Sau96I GGNCC 4 cut(s) 389, 419, 712, 1415
ScrFI CCNGG 4 cut(s) 424, 425, 492, 1067
SduI GDGCHC 2 cut(s) 206, 347
SfaNI GCATC 4 cut(s) 1180, 1202, 1343, 1523
SfuI TTCGAA 1 cut(s) 661
SinI GGWCC 3 cut(s) 389, 419, 712
SmaI CCCGGG 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 1307
Sse9I AATT 8 cut(s) 262, 274, 564, 738, 834, 954, 1082, 1446
SsiI CCGC 6 cut(s) 124, 525, 645, 767, 1071, 1096
SspMI CTAG 3 cut(s) 393, 1503, 1530
StyD4I CCNGG 4 cut(s) 422, 423, 490, 1065
StyI CCWWGG 1 cut(s) 1386
TaaI ACNGT 7 cut(s) 505, 980, 1352, 1411, 1427, 1477, 1547
TaiI ACGT 4 cut(s) 249, 415, 799, 1119
TaqI TCGA 6 cut(s) 240, 661, 1015, 1089, 1215, 1315
TaqII GACCGA 1 cut(s) 729
TasI AATT 8 cut(s) 262, 274, 564, 738, 834, 954, 1082, 1446
TatI WGTACW 1 cut(s) 1352
TauI GCSGC 2 cut(s) 528, 1099
TfiI GAWTC 7 cut(s) 5, 208, 508, 1036, 1043, 1312, 1395
Tru1I TTAA 4 cut(s) 909, 1101, 1232, 1245
Tru9I TTAA 4 cut(s) 909, 1101, 1232, 1245
TscAI CASTG 3 cut(s) 985, 1405, 1552
TseFI GTSAC 2 cut(s) 469, 1456
TseI GCWGC 2 cut(s) 914, 1029
Tsp45I GTSAC 2 cut(s) 469, 1456
TspDTI ATGAA 7 cut(s) 6, 465, 626, 976, 1184, 1301, 1367
TspGWI ACGGA 4 cut(s) 87, 96, 153, 158
TspMI CCCGGG 1 cut(s) 423
TspRI CASTG 3 cut(s) 985, 1405, 1552
VpaK11BI GGWCC 3 cut(s) 389, 419, 712
VspI ATTAAT 1 cut(s) 909
XapI RAATTY 1 cut(s) 1082
XceI RCATGY 4 cut(s) 541, 923, 1001, 1436
XmaI CCCGGG 1 cut(s) 423
XmiI GTMKAC 2 cut(s) 732, 1429
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 3 cut(s) 393, 1503, 1530
Zsp2I ATGCAT 2 cut(s) 1195, 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.