pycom01g19150

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012250
Physical Location & Seq
Forward (+)
17952927 .. 17956971
4045 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3111 bp
ATGAAAGTACCTTCCTTTTCCTCCTCCTCCTCCTTCCCTCTCTCTCTCCTCTTCTTCTTCTCTCTCCAAACCTCCATAACTCTCTGCTCTTCTTCCTCTAACTCTTCTGTGTCCTTCCAGCTCATTGCTCTGCTCTCCCTCAAATCCTCCCTGAAAGACCCTCTCTCCTCCTTCCACGACTGGGTTTCCCCCAATTCCCACCCAAACGGCAGCGTCTCCGGTTGGTGTTCGTGGTCCGGCGTCAAATGCCAACCGGACACCTCCCAAGTTGTTTCCCTCGACCTCTCCCACCGCAACCTCTCCGGACAAATCCCACCACAAATCCGCCACCTTTCTTCACTAATCCACCTCAATTTGAGCGGCAACGCCTTCCACGGCCCTCTCCAGCCCGCCATTTTCGAGCTCGACAACCTCAGGATCCTCGACCTCAGCCACAACGACTTCAAGTCGGATTTCCCGCCCGGGATTTCGAAGCTCAGGTTTTTAAGAGTCTTTACCGCTTACAGTAACAGCTTCACTGGCCCATTGCCTCAAGAATTCGTCAAGCTCCGCTACCTCGAGCAGCTCAACCTCGGTGGAAGTTACTTCGATGGTAAAATCCCAGAAGGGTATGGAACATTCCCCAAGTTAAAATTGCTCTACCTCGCCGGAAATGCACTCAACGGTCCAATTCCGCCGCAATTGGGTTCCTTATCGGAGCTGACTCGGATGGAGATCGGGTACAACGTGTATTCAGGTGGGCTTCCTGCAGAACTCGGAATGCTGTCAAATCTCACGTACTTGGACGTCTCCAACGCCTTTCTCTCCGGCCCTCTCCCGCCGGAGCTCGGCAACTTAACCATGCTTGACACATTGCTGCTCTTCAAGAACCGATTTTCAGGCTTTATCCCATCAACTATCGGCCTCTTACAGAGACTGAAGACGCTCGACTTGTCGGATAATCAGCTTTCCGGCTCAATCCCGCCGGGAATTGGTACACTGAAAGAGCTGACCATGATAAGCTTGATGAACAACTTTCTCGTCGGAGAAATCCCGGAAAAAATCGGAGAGCTGCCCAACTTGGAAAACCTCCTCCTCTGGAACAATTCGCTGACGGGTCTCCTTCCCCAGGGCTTAGGATTCAGCGGGAAGCTCCTGCGAGTTGACGTCTCCTCCAATTCTCTCACCGGCCCGATCCCGCCAAATCTCTGCGACGGCAACAAGCTCCAAAAGCTCATACTTTTCTCCAACCGGTTCACCGACCCTCTTCCCAAAACCTTAGCAAACTGCACTTCTCTGTTCCGACTCCGAATCCAGAACAACCAAATCAACGGGTCAGTTCCGACGGGTTTCGGGTTATTACCCAATCTAACCTTCGTCGATTTGAGCGGCAACAATTTCACAGGCACGATCCCGGAAGATCTCGGCAATGCCGAAGCTCTCAGTTACTTGAACATTTCGCTGAACCCATTGCACTCAGTCCTTCCGTCAAACATCTGGCGTGCACCAAATCTGCAGATCTTCTCTGCTAGCTCCGGCCACCTCACAGGAAAAATCCCCGACTTCATCGGCTGCCAAAGCCTGTACAAGATCGAACTTCAACGTAACGACTTTAACGGCTCGATCCCATTGGACATCGGGCACTGCGAGAAGCTTCTCAGCTTGAATCTAAGCCGCAATTCTCTCACCGGCATTATCCCACGGGAAATCTCCGCTTTGCCCTCCATCACCGACCTCGACCTCTCCCGGAACTTTCTCTCCGGCACGATCCCGTCCAACTTCGAGAACTGTAGCACGTTGGAGAGCTTCAACGTTTCGTTTAATTTGTTGAACGGTCCGATCCCCGCGTCCGGTTCCATTTTCCCGAATCTCCATCCGTCGTCGTTTTTGGGGAATAAAGGTTTATGTGGCGGCGTTTTGGGGAAGCCGTGTGACACCTTGTCGGCCGGAGCCATGGAGGTTCGCGGCCACGAGCAGCCGAAGAAGACGGCGGGCGCGATAGTCTGGATCATGGCCGCTGCTTTCGGTATCGGGCTTTTTGTTCTCATCGCTGGGACCCGCTGCTTCCGCGCGAATTACAGCCGCCGGATGGACGAGAGTCAGCAGATTGGACCGTGGAAATTAACCGCTTTCCAGCGGTTAAATTTCACGGCGGATGATGTGTTGGAGTGTTTGGAGATGAGTGATAAGATCATAGGGATGGGGTCCACGGGGACGGTTTATAGAGCGGAGATGCCAAGTGGCGAGTTTATAGCCGTTAAGAAGCTGTGGGGAAAACAGAAGGAGAACAATAGTGATCTGATCCGACGGCGGAGAGGGGTCCTGGCTGAGGTGGAGGTGTTGGGGAACGTGAGGCACAGGAATATAGTAAGACTGTTGGGGTGCTGCTGCAACAGGGACATCACCATGCTGCTCTACGAGTACATGCCCAACGGGAATTTGGATGATCTGTTGCATGGCAAAAACAAGGCGCAGAATTTGGGTGCGGATTGGGTCACGAGGTACAAGATCGCCCTCGGCGTGGCGCAGGGGATTTGTTACCTGCACCATGACTGTAACCCCGTGATTGTGCACCGTGATCTTAAGCCGAGTAATATCTTGTTGGATAGCGAGATGGAGGCTAGAGTGGCGGATTTTGGGGTGGCCAAGTTGATCCAGAGCGATGAGTCCATGTCCGTCATCGCCGGCTCCTATGGCTACATTGCTCCAGAATATGCTTATACCCTACAAGTGGATGAAAAGAGTGACATATACAGTTATGGTGTGGTGTTGATGGAGATTTTAAGCGGGAAAAGATCGGTGGACGCCGAGTTCGGAGATGGGAACAGCATAGTGGATTGGGTTAGGTCGAAGATCAAGACGAAAGACGGGATCAACGATATTTTGGACAAGAATGCTGGGGCGGGTTGTGCGCCGGTGAGGGAGGAGATGATGCAGATGCTTAGGATTGCTTTGCTCTGCACGAGCCGGAACCCGGCAGACCGGCCGTCGATGAGGGACGTGGTGTTGATGCTGACTGAGGCCAAGCCCAAGAGGAAGGTCCTAGGCAATGTTGGGCATGGTCACAATGGGAGTGGTTGTGCTGAATATCATGATATTCCCTTGCCACAGAAGCCGTCGGTCGAATGTTAA

Protein Analysis

1037

Amino Acids

113.19

Weight (kDa)

6.85

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 251 - 357 7.5e-09 Leucine-rich repeat region
LRR_8 PF13855 401 - 460 1.8e-06 Leucine rich repeat
LRR_8 PF13855 520 - 577 1.5e-07 Leucine rich repeat
Pkinase PF00069 722 - 994 4.4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 722 - 996 9.2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61480
fragaria_vesca FvH4_7g27270
malus_domestica MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica Prupe.2G275500_v2.0.a1
pyrus_communis pycom01g19150 pycom07g22430
rosa_chinensis RchiOBHm_Chr1g0374101
rosa_laevigata RLG00000026763
rosa_multiflora Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii Rroxscaffold_4G00283740
rosa_rugosa Rorug01G0380800
rosa_samantha Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana Rw1G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 789, 1149
Acc36I ACCTGC 1 cut(s) 2529
AccBSI CCGCTC 3 cut(s) 360, 1368, 2207
AccII CGCG 5 cut(s) 1826, 1944, 1976, 2049, 2051
AccIII TCCGGA 1 cut(s) 302
AclI AACGTT 1 cut(s) 1791
AcoI YGGCCR 6 cut(s) 1516, 1923, 1945, 1992, 2622, 2963
AcsI RAATTY 4 cut(s) 536, 2122, 2416, 2455
AcuI CTGAAG 1 cut(s) 938
AcyI GRCGYC 4 cut(s) 240, 786, 1146, 2784
AfaI GTAC 7 cut(s) 9, 722, 779, 976, 1565, 2402, 2483
AflII CTTAAG 1 cut(s) 2561
AflIII ACRYGT 1 cut(s) 726
AgeI ACCGGT 1 cut(s) 1230
AgsI TTSAA 7 cut(s) 445, 865, 1432, 1580, 1645, 1789, 1810
AhdI GACNNNNNGTC 3 cut(s) 445, 1918, 2965
AjiI CACGTC 1 cut(s) 2980
AjnI CCWGG 2 cut(s) 1107, 2301
AjuI GAANNNNNNNTTGG 2 cut(s) 1337, 1369
AloI GAACNNNNNNTCC 4 cut(s) 1721, 1753, 2774, 2806
Alw21I GWGCWC 4 cut(s) 405, 828, 1486, 2553
Alw26I GTCTC 5 cut(s) 220, 793, 907, 1103, 1153
Alw44I GTGCAC 2 cut(s) 1482, 2549
AlwNI CAGNNNCTG 1 cut(s) 916
Ama87I CYCGRG 2 cut(s) 461, 557
Aor13HI TCCGGA 1 cut(s) 302
ApaLI GTGCAC 2 cut(s) 1482, 2549
ApoI RAATTY 4 cut(s) 536, 2122, 2416, 2455
ArsI GACNNNNNNTTYG 2 cut(s) 2774, 2806
AsiGI ACCGGT 1 cut(s) 1230
AspA2I CCTAGG 1 cut(s) 3022
AspLEI GCGC 5 cut(s) 1976, 2051, 2452, 2506, 2893
AsuC2I CCSGG 7 cut(s) 462, 463, 966, 1034, 1394, 1726, 2954
AsuHPI GGTGA 6 cut(s) 1156, 1228, 1657, 1699, 2374, 2908
AsuII TTCGAA 1 cut(s) 470
AsuNHI GCTAGC 1 cut(s) 1508
AvaI CYCGRG 2 cut(s) 461, 557
AvaII GGWCC 8 cut(s) 234, 665, 1814, 2034, 2090, 2184, 2299, 3019
AvrII CCTAGG 1 cut(s) 3022
AxyI CCTNAGG 1 cut(s) 413
BaeGI GKGCMC 3 cut(s) 1486, 1623, 2553
BalI TGGCCA 1 cut(s) 2624
BamHI GGATCC 1 cut(s) 417
BanII GRGCYC 2 cut(s) 405, 828
BauI CACGAG 3 cut(s) 1949, 2476, 2941
BbsI GAAGAC 2 cut(s) 926, 1970
Bbv12I GWGCWC 4 cut(s) 405, 828, 1486, 2553
BbvCI CCTCAGC 2 cut(s) 428, 2307
BciT130I CCWGG 2 cut(s) 1109, 2303
BcnI CCSGG 7 cut(s) 462, 463, 966, 1034, 1394, 1726, 2954
BcoDI GTCTC 5 cut(s) 220, 793, 907, 1103, 1153
BfaI CTAG 3 cut(s) 1509, 2601, 3023
BfmI CTRYAG 3 cut(s) 747, 1493, 1768
BfrI CTTAAG 1 cut(s) 2561
BfuAI ACCTGC 1 cut(s) 2529
BglII AGATCT 2 cut(s) 1399, 1497
BlnI CCTAGG 1 cut(s) 3022
Bme1390I CCNGG 9 cut(s) 462, 463, 966, 1034, 1109, 1394, 1726, 2303, 2954
Bme18I GGWCC 8 cut(s) 234, 665, 1814, 2034, 2090, 2184, 2299, 3019
BmeRI GACNNNNNGTC 3 cut(s) 445, 1918, 2965
BmeT110I CYCGRG 2 cut(s) 461, 557
BmgBI CACGTC 1 cut(s) 2980
BmrFI CCNGG 9 cut(s) 462, 463, 966, 1034, 1109, 1394, 1726, 2303, 2954
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 4 cut(s) 2202, 2901, 2907, 2979
BmtI GCTAGC 1 cut(s) 1512
BmuI ACTGGG 1 cut(s) 190
BoxI GACNNNNGTC 1 cut(s) 2076
BpiI GAAGAC 2 cut(s) 926, 1970
BpmI CTGGAG 2 cut(s) 368, 2670
Bpu10I CCTNAGC 6 cut(s) 428, 476, 1114, 1258, 2307, 2921
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 7 cut(s) 462, 463, 966, 1034, 1394, 1726, 2954
BsaAI YACGTR 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 713
BsaHI GRCGYC 4 cut(s) 240, 786, 1146, 2784
BsaI GGTCTC 1 cut(s) 1103
BsaWI WCCGGW 5 cut(s) 218, 253, 302, 1230, 1829
Bse118I RCCGGY 6 cut(s) 1166, 1230, 1667, 2663, 2893, 2961
Bse1I ACTGG 2 cut(s) 185, 523
Bse21I CCTNAGG 1 cut(s) 413
Bse3DI GCAATG 7 cut(s) 123, 524, 851, 1414, 1448, 2679, 3034
Bse8I GATNNNNATC 1 cut(s) 713
BseAI TCCGGA 1 cut(s) 302
BseBI CCWGG 2 cut(s) 1109, 2303
BseGI GGATG 7 cut(s) 714, 1852, 2073, 2140, 2184, 2428, 2719
BseJI GATNNNNATC 1 cut(s) 713
BseMI GCAATG 7 cut(s) 123, 524, 851, 1414, 1448, 2679, 3034
BseMII CTCAG 8 cut(s) 427, 442, 490, 1435, 1470, 1651, 2298, 2988
BseNI ACTGG 2 cut(s) 185, 523
BseRI GAGGAG 9 cut(s) 13, 16, 19, 38, 157, 1061, 1064, 1141, 2918
BseSI GKGCMC 3 cut(s) 1486, 1623, 2553
BseX3I CGGCCG 2 cut(s) 1923, 2963
BseYI CCCAGC 2 cut(s) 2030, 2876
BsgI GTGCAG 3 cut(s) 1252, 2507, 2923
Bsh1236I CGCG 5 cut(s) 1826, 1944, 1976, 2049, 2051
Bsh1285I CGRYCG 3 cut(s) 1926, 2966, 3102
BshTI ACCGGT 1 cut(s) 1230
BsiEI CGRYCG 3 cut(s) 1926, 2966, 3102
BsiHKAI GWGCWC 4 cut(s) 405, 828, 1486, 2553
BsiHKCI CYCGRG 2 cut(s) 461, 557
BslFI GGGAC 4 cut(s) 2047, 2206, 2390, 2990
BsmAI GTCTC 5 cut(s) 220, 793, 907, 1103, 1153
BsmBI CGTCTC 3 cut(s) 220, 793, 1153
BsmFI GGGAC 4 cut(s) 2047, 2206, 2390, 2990
BsmI GAATGC 2 cut(s) 765, 2878
Bso31I GGTCTC 1 cut(s) 1103
BsoBI CYCGRG 2 cut(s) 461, 557
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 5 cut(s) 405, 828, 1486, 1623, 2553
Bsp13I TCCGGA 1 cut(s) 302
Bsp1407I TGTACA 1 cut(s) 1563
Bsp19I CCATGG 1 cut(s) 1932
BspCNI CTCAG 8 cut(s) 426, 441, 489, 1434, 1469, 1650, 2299, 2989
BspEI TCCGGA 1 cut(s) 302
BspFNI CGCG 5 cut(s) 1826, 1944, 1976, 2049, 2051
BspHI TCATGA 1 cut(s) 3070
BspMAI CTGCAG 2 cut(s) 751, 1497
BspMI ACCTGC 1 cut(s) 2529
BspOI GCTAGC 1 cut(s) 1512
BspQI GCTCTTC 2 cut(s) 94, 866
BspT104I TTCGAA 1 cut(s) 470
BspTI CTTAAG 1 cut(s) 2561
BspTNI GGTCTC 1 cut(s) 1103
BsrBI CCGCTC 3 cut(s) 360, 1368, 2207
BsrDI GCAATG 7 cut(s) 123, 524, 851, 1414, 1448, 2679, 3034
BsrFI RCCGGY 6 cut(s) 1166, 1230, 1667, 2663, 2893, 2961
BsrGI TGTACA 1 cut(s) 1563
BsrI ACTGG 2 cut(s) 185, 523
BssAI RCCGGY 6 cut(s) 1166, 1230, 1667, 2663, 2893, 2961
BssNI GRCGYC 4 cut(s) 240, 786, 1146, 2784
BssSI CACGAG 3 cut(s) 1949, 2476, 2941
BssT1I CCWWGG 2 cut(s) 1932, 3022
Bst2BI CACGAG 3 cut(s) 1949, 2476, 2941
Bst2UI CCWGG 2 cut(s) 1109, 2303
Bst6I CTCTTC 5 cut(s) 56, 94, 109, 866, 1251
BstACI GRCGYC 4 cut(s) 240, 786, 1146, 2784
BstAFI CTTAAG 1 cut(s) 2561
BstAUI TGTACA 1 cut(s) 1563
BstBAI YACGTR 1 cut(s) 777
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 5 cut(s) 390, 1482, 1510, 1972, 2665
BstDSI CCRYGG 5 cut(s) 373, 1679, 1932, 2093, 2187
BstENI CCTNNNNNAGG 2 cut(s) 1106, 2306
BstF5I GGATG 7 cut(s) 714, 1852, 2073, 2140, 2184, 2428, 2719
BstFNI CGCG 5 cut(s) 1826, 1944, 1976, 2049, 2051
BstHHI GCGC 5 cut(s) 1976, 2051, 2452, 2506, 2893
BstMAI GTCTC 5 cut(s) 220, 793, 907, 1103, 1153
BstMCI CGRYCG 3 cut(s) 1926, 2966, 3102
BstNI CCWGG 2 cut(s) 1109, 2303
BstNSI RCATGY 1 cut(s) 2407
BstPAI GACNNNNGTC 1 cut(s) 2076
BstSCI CCNGG 9 cut(s) 460, 461, 964, 1032, 1107, 1392, 1724, 2301, 2952
BstSFI CTRYAG 3 cut(s) 747, 1493, 1768
BstSLI GKGCMC 3 cut(s) 1486, 1623, 2553
BstUI CGCG 5 cut(s) 1826, 1944, 1976, 2049, 2051
BstV2I GAAGAC 2 cut(s) 926, 1970
BstX2I RGATCY 3 cut(s) 417, 1399, 1497
BstYI RGATCY 3 cut(s) 417, 1399, 1497
BstZI CGGCCG 2 cut(s) 1923, 2963
Bsu36I CCTNAGG 1 cut(s) 413
BtgI CCRYGG 5 cut(s) 373, 1679, 1932, 2093, 2187
BtgZI GCGATG 3 cut(s) 2011, 2644, 2655
BtrI CACGTC 1 cut(s) 2980
BtsCI GGATG 7 cut(s) 714, 1852, 2073, 2140, 2184, 2428, 2719
BtsI GCAGTG 1 cut(s) 1621
BtsIMutI CAGTG 3 cut(s) 516, 977, 1621
BveI ACCTGC 1 cut(s) 2529
Cac8I GCNNGC 5 cut(s) 390, 1482, 1510, 1972, 2665
CaiI CAGNNNCTG 1 cut(s) 916
CciI TCATGA 1 cut(s) 3070
CfoI GCGC 5 cut(s) 1976, 2051, 2452, 2506, 2893
Cfr10I RCCGGY 6 cut(s) 1166, 1230, 1667, 2663, 2893, 2961
Cfr9I CCCGGG 1 cut(s) 461
CpoI CGGWCCG 1 cut(s) 1814
CseI GACGC 5 cut(s) 202, 229, 931, 1815, 2792
Csp6I GTAC 7 cut(s) 8, 721, 778, 975, 1564, 2401, 2482
CspAI ACCGGT 1 cut(s) 1230
CspCI CAANNNNNGTGG 4 cut(s) 556, 591, 3034, 3069
CspI CGGWCCG 1 cut(s) 1814
CviQI GTAC 7 cut(s) 8, 721, 778, 975, 1564, 2401, 2482
DriI GACNNNNNGTC 3 cut(s) 445, 1918, 2965
EaeI YGGCCR 6 cut(s) 1516, 1923, 1945, 1992, 2622, 2963
EagI CGGCCG 2 cut(s) 1923, 2963
Eam1104I CTCTTC 5 cut(s) 56, 94, 109, 866, 1251
Eam1105I GACNNNNNGTC 3 cut(s) 445, 1918, 2965
EarI CTCTTC 5 cut(s) 56, 94, 109, 866, 1251
EciI GGCGGA 5 cut(s) 314, 663, 2147, 2305, 2624
Ecl136II GAGCTC 2 cut(s) 403, 826
EclXI CGGCCG 2 cut(s) 1923, 2963
Eco130I CCWWGG 2 cut(s) 1932, 3022
Eco24I GRGCYC 2 cut(s) 405, 828
Eco31I GGTCTC 1 cut(s) 1103
Eco47I GGWCC 8 cut(s) 234, 665, 1814, 2034, 2090, 2184, 2299, 3019
Eco52I CGGCCG 2 cut(s) 1923, 2963
Eco53kI GAGCTC 2 cut(s) 403, 826
Eco57I CTGAAG 1 cut(s) 938
Eco81I CCTNAGG 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 461, 557
EcoICRI GAGCTC 2 cut(s) 403, 826
EcoNI CCTNNNNNAGG 2 cut(s) 1106, 2306
EcoO109I RGGNCCY 3 cut(s) 2034, 2299, 3019
EcoRI GAATTC 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 1107, 2301
EcoT14I CCWWGG 2 cut(s) 1932, 3022
EcoT38I GRGCYC 2 cut(s) 405, 828
ErhI CCWWGG 2 cut(s) 1932, 3022
Esp3I CGTCTC 3 cut(s) 220, 793, 1153
FaqI GGGAC 4 cut(s) 2047, 2206, 2390, 2990
FokI GGATG 7 cut(s) 721, 1839, 2080, 2147, 2191, 2435, 2726
FriOI GRGCYC 2 cut(s) 405, 828
FspBI CTAG 3 cut(s) 1509, 2601, 3023
GlaI GCGC 5 cut(s) 1975, 2050, 2451, 2505, 2892
GsaI CCCAGC 2 cut(s) 2034, 2880
GsuI CTGGAG 2 cut(s) 368, 2670
HgaI GACGC 5 cut(s) 202, 229, 931, 1815, 2792
HhaI GCGC 5 cut(s) 1976, 2051, 2452, 2506, 2893
Hin1I GRCGYC 4 cut(s) 240, 786, 1146, 2784
Hin6I GCGC 5 cut(s) 1974, 2049, 2450, 2504, 2891
HinP1I GCGC 5 cut(s) 1974, 2049, 2450, 2504, 2891
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HindIII AAGCTT 2 cut(s) 1000, 1631
HinfI GANTC 9 cut(s) 489, 703, 1119, 1284, 1290, 1645, 1846, 2077, 2645
HphI GGTGA 6 cut(s) 1156, 1228, 1657, 1699, 2374, 2908
Hpy166II GTNNAC 7 cut(s) 977, 1144, 1236, 1484, 2187, 2551, 2782
Hpy8I GTNNAC 7 cut(s) 977, 1144, 1236, 1484, 2187, 2551, 2782
Hpy99I CGWCG 9 cut(s) 1025, 1196, 1327, 1361, 1861, 1864, 2289, 2971, 3100
HpyCH4IV ACGT 9 cut(s) 726, 776, 786, 1146, 1582, 1775, 1791, 2328, 2979
HpySE526I ACGT 9 cut(s) 726, 776, 786, 1146, 1582, 1775, 1791, 2328, 2979
Hsp92I GRCGYC 4 cut(s) 240, 786, 1146, 2784
HspAI GCGC 5 cut(s) 1974, 2049, 2450, 2504, 2891
KflI GGGWCCC 1 cut(s) 2034
Kpn2I TCCGGA 1 cut(s) 302
KroI GCCGGC 1 cut(s) 2663
KroNI GCCGGC 1 cut(s) 2665
LguI GCTCTTC 2 cut(s) 94, 866
LmnI GCTCC 9 cut(s) 552, 697, 823, 1137, 1209, 1517, 1928, 2672, 2689
LweI GCATC 4 cut(s) 2202, 2901, 2907, 2979
MaeI CTAG 3 cut(s) 1509, 2601, 3023
MaeII ACGT 9 cut(s) 726, 776, 786, 1146, 1582, 1775, 1791, 2328, 2979
MbiI CCGCTC 3 cut(s) 360, 1368, 2207
MfeI CAATTG 1 cut(s) 680
MflI RGATCY 3 cut(s) 417, 1399, 1497
MhlI GDGCHC 5 cut(s) 405, 828, 1486, 1623, 2553
MlsI TGGCCA 1 cut(s) 2624
MluNI TGGCCA 1 cut(s) 2624
MlyI GAGTC 5 cut(s) 498, 697, 1278, 2086, 2654
Mox20I TGGCCA 1 cut(s) 2624
MroI TCCGGA 1 cut(s) 302
MroNI GCCGGC 1 cut(s) 2663
MscI TGGCCA 1 cut(s) 2624
MslI CAYNNNNRTG 2 cut(s) 2177, 2384
Msp20I TGGCCA 1 cut(s) 2624
MspA1I CMGCKG 4 cut(s) 1125, 1997, 2040, 2116
MspCI CTTAAG 1 cut(s) 2561
MspR9I CCNGG 9 cut(s) 462, 463, 966, 1034, 1109, 1394, 1726, 2303, 2954
MunI CAATTG 1 cut(s) 680
Mva1269I GAATGC 2 cut(s) 765, 2878
MvaI CCWGG 2 cut(s) 1109, 2303
MvnI CGCG 5 cut(s) 1826, 1944, 1976, 2049, 2051
NaeI GCCGGC 1 cut(s) 2665
NciI CCSGG 7 cut(s) 462, 463, 966, 1034, 1394, 1726, 2954
NcoI CCATGG 1 cut(s) 1932
NgoMIV GCCGGC 1 cut(s) 2663
NheI GCTAGC 1 cut(s) 1508
NmeAIII GCCGAG 5 cut(s) 807, 1383, 2475, 2592, 2812
NmuCI GTSAC 4 cut(s) 1910, 2473, 2723, 3041
NspI RCATGY 1 cut(s) 2407
NspV TTCGAA 1 cut(s) 470
PaeR7I CTCGAG 1 cut(s) 557
PagI TCATGA 1 cut(s) 3070
PasI CCCWGGG 1 cut(s) 1108
PciSI GCTCTTC 2 cut(s) 94, 866
PcsI WCGNNNNNNNCGW 6 cut(s) 723, 1410, 1623, 1973, 2496, 2853
PctI GAATGC 2 cut(s) 765, 2878
PdiI GCCGGC 1 cut(s) 2665
PfeI GAWTC 4 cut(s) 1119, 1290, 1645, 1846
PfoI TCCNGGA 3 cut(s) 1032, 1392, 1724
PinAI ACCGGT 1 cut(s) 1230
PleI GAGTC 5 cut(s) 497, 697, 1278, 2085, 2653
PpsI GAGTC 5 cut(s) 497, 697, 1278, 2085, 2653
Ppu21I YACGTR 1 cut(s) 777
PpuMI RGGWCCY 3 cut(s) 2034, 2299, 3019
PshAI GACNNNNGTC 1 cut(s) 2076
Psp124BI GAGCTC 2 cut(s) 405, 828
Psp1406I AACGTT 1 cut(s) 1791
Psp5II RGGWCCY 3 cut(s) 2034, 2299, 3019
Psp6I CCWGG 2 cut(s) 1107, 2301
PspFI CCCAGC 2 cut(s) 2030, 2876
PspGI CCWGG 2 cut(s) 1107, 2301
PspPPI RGGWCCY 3 cut(s) 2034, 2299, 3019
PspXI VCTCGAGB 1 cut(s) 557
PstI CTGCAG 2 cut(s) 751, 1497
PstNI CAGNNNCTG 1 cut(s) 916
PsuI RGATCY 3 cut(s) 417, 1399, 1497
RsaI GTAC 7 cut(s) 9, 722, 779, 976, 1565, 2402, 2483
RsaNI GTAC 7 cut(s) 8, 721, 778, 975, 1564, 2401, 2482
RseI CAYNNNNRTG 2 cut(s) 2177, 2384
Rsr2I CGGWCCG 1 cut(s) 1814
RsrII CGGWCCG 1 cut(s) 1814
SacI GAGCTC 2 cut(s) 405, 828
SapI GCTCTTC 2 cut(s) 94, 866
SchI GAGTC 5 cut(s) 498, 697, 1278, 2086, 2654
ScrFI CCNGG 9 cut(s) 462, 463, 966, 1034, 1109, 1394, 1726, 2303, 2954
SduI GDGCHC 5 cut(s) 405, 828, 1486, 1623, 2553
SfaNI GCATC 4 cut(s) 2202, 2901, 2907, 2979
SfcI CTRYAG 3 cut(s) 747, 1493, 1768
Sfr274I CTCGAG 1 cut(s) 557
SfuI TTCGAA 1 cut(s) 470
SgrAI CRCCGGYG 1 cut(s) 2893
SinI GGWCC 8 cut(s) 234, 665, 1814, 2034, 2090, 2184, 2299, 3019
SlaI CTCGAG 1 cut(s) 557
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 2 cut(s) 2177, 2384
SmlI CTYRAG 3 cut(s) 531, 557, 2561
SmoI CTYRAG 3 cut(s) 531, 557, 2561
SspMI CTAG 3 cut(s) 1509, 2601, 3023
SstI GAGCTC 2 cut(s) 405, 828
StyD4I CCNGG 9 cut(s) 460, 461, 964, 1032, 1107, 1392, 1724, 2301, 2952
StyI CCWWGG 2 cut(s) 1932, 3022
TaiI ACGT 9 cut(s) 729, 779, 789, 1149, 1585, 1778, 1794, 2331, 2982
TaqII GACCGA 1 cut(s) 3088
TatI WGTACW 2 cut(s) 1563, 2400
TauI GCSGC 8 cut(s) 363, 679, 1371, 1656, 1893, 1947, 1997, 2064
TfiI GAWTC 4 cut(s) 1119, 1290, 1645, 1846
TscAI CASTG 3 cut(s) 523, 984, 1628
TseFI GTSAC 4 cut(s) 1910, 2473, 2723, 3041
Tsp45I GTSAC 4 cut(s) 1910, 2473, 2723, 3041
TspDTI ATGAA 4 cut(s) 17, 1022, 1534, 2730
TspGWI ACGGA 3 cut(s) 1455, 1845, 2644
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 3 cut(s) 523, 984, 1628
Vha464I CTTAAG 1 cut(s) 2561
VneI GTGCAC 2 cut(s) 1482, 2549
VpaK11BI GGWCC 8 cut(s) 234, 665, 1814, 2034, 2090, 2184, 2299, 3019
XagI CCTNNNNNAGG 2 cut(s) 1106, 2306
XapI RAATTY 4 cut(s) 536, 2122, 2416, 2455
XceI RCATGY 1 cut(s) 2407
XcmI CCANNNNNNNNNTGG 1 cut(s) 2416
XhoI CTCGAG 1 cut(s) 557
XmaI CCCGGG 1 cut(s) 461
XmaJI CCTAGG 1 cut(s) 3022
XspI CTAG 3 cut(s) 1509, 2601, 3023
ZraI GACGTC 2 cut(s) 787, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.