pycom01g22370

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007218
Physical Location & Seq
Reverse (-)
19993323 .. 20000754
7432 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3972 bp
ATGGCCACATCAGCTAAAAAAAACCTTGATCTGATCGCTCTCGCGCCAAATTCCTCCACTCCTCATCTCCTCCAGAAATCCCAAACTCTCCAACCCTCGTTTGAAAACGGTACCGTTTCACGGAAGATGGACTTGGAAGGCTTATCGGTAATATGCTGTGATCTGGGGATTGCAGAAGAAGATGACAATGGCAACCGGATCGGCTACTCCAAGGGCAAGTACTGCTTAGATAATCTGAAAGATTTGCTAAGGTTTTTGAGGCGTGATGACCCGCAGAATCGGGATGTTTTTAAGCAAGTCTGTAAATGGAATATTGTAGATCAGCATTTGGTACCTATCATTGAACACTGCCAAGCTGATACCAACTTGGTTTTGAATGCAGGTAAAATCGTTTTTCTCTCTTCATTACATGTTTATGAAAACGTATTCCAAAGGGACGACTTTAATTATTTTTTTGAATTTGCAGTGAAGGTTCTGGTGTTCCTAACTATGCCAGTTGAGCCTTCGTCGAATGATGTTTTGCAACAGATAGAGTTTCTGTGGAAGTTGAAGTCTTCGATTACATCTAGTGATACTGTTGCAGTTATAGTATCACTTCTGGAAAGCCCACTGGAAAATTTGGAACGTGATGCATTCACGGAGGATGACTGGAAACTGGTGCAGCTGGTACTTACTTTATTCCGAAACATCTTAGCTGTTCAAGAAATTTCACAGCAGCAGAAGGCTGGAGGAACGGCCATTCAATTTGTATCTCTCAGAGATAGATTTCTAGAACTTTTGTTTCATGAGAACGTGATGGACATAGTTTTAGTCATAACCCAGCATATTGGTGATTCCCGTAGCTATCTCTGTCATGATAACTTGCTTTTATTGGAAATTTTCCATTACATATTCATGGGTCAAGAACCAGAATTGATTGCAAACGCATATTCAAAAGGTCCTAAGGTTGGTTTCTCTTCCTCTCTTTCTGAATTCTTTCCAAGGAAAATGAGGTTTGTGGATGGAAACACTGCTGTTTCTCTAAATAGTCTCAAGTCCATAATGGAGGAAGAAGAAGAAGAGAAAAAAAGACTTTCTAGACTACGTAACATGAGTCGCCATTCACATTTTAGTGGAACATTTACCCAGCTCACCATGGATGGTTCTGTGGCAGTTTTGAAGGGAAGGCCTACTTCTACCTCTTGTAAAACCATGCTTAAACCACATAATCCCCGGGGTCCAGTAAAAAAAATTGCTTGGGATCATGGGACATTGCCTTCAACAAAGGATAAGATTTTAGAATTGCTCCATGATTTTGTAAACCAGTTTTTATCAGGGGGTTACAATGTTCTGATGCAGTCAATTCGTGAGAATATTGAAAAGGAACACCCCTCAATTCAGAAGAGTGATGTTGTTGTCTTCTTTCAAGTTGCTCAGTTTGCTATTTCGTTCCAGTATCACAAGTCTTCAATTTCCAAGCCAAGCATGGGCACAGAAGCAGATCCAACTGTAGCTCCCACTGATAAAGATGCTGATAGCACATTCTTCAAAGGTGATGTTTGTGGACCCATTGCAGCATCAGTGAACGAATCTATGTTTCAACTAGTCATTTCAAAGTGGCGTTATGCATATGATAGCTTGAAGGAAACACATGACTACAAGTTTTTGTCTGCAGCTGGTTCTCTTATGAAAAATATGATTCGCATGCTAGATTTGGTGCTTAAGTTATTGCCAGCAGATTCCAAGGAGCCTCAAACAGCTCGCATACTTCTCTACAAATTATTTTATGATCAGACTGATGAGGGAATGACTCACTTCCTCATAAATTTACTCAAATCATTTGACACTTACAAACAACCCAGAAGTGATCTTGCAGATTTGATAGAGATGGTTTATAAAGTTTTACGGCTCATGGAAAGCCTTCAAGCAGGCGGAACTTTGAGGGTTTCTAAAAAATCTAGGAAAGTGAGGAAGAAGAAAACACCGATCGAGAAGGACACAGAAAATAAACTCTTAGAAGAACACGCTACCATTCAAAAAGAGATTGGCATCTCCAATGAAGAGCAATCAACAGATGTGAGTGTGACAGAGAACAGAAGCCTAAACACAATTTCTGATGGGAAAGAAGACACCATTATCCCTGATCAGCCTGATGAATGCAAAATATCTTTGTTGGAGACAGAAAAAATTGAAAGTCTGGCACAGATCGATCGCAGAGATTCTGACTTTGTCAATGGTGATCTTGGTTATGGCACAGGCGATTCTTCTGCTGATGAGCAGGTAGCTGCAACTGATGAAGTTGATTTTAAGGTGTCAACTTTGATTTCTGCTTTTGCAAGCCACAGCATCATCCAGAAGTTGTGTTGGTTGCTTAAGTTTTATAAGACTAACTCAACCAGTACAAATCACTACATAGTTAGCATGCTTCGAAGGATCAGTGATGACCTGGGGCTCTCTCCGATGTTGTATCAGTTATCACTCCTCACTACATTCTATGACATTCTAGCTGAGCAGAAGTCATGTCCATGCAAGGCGTTCGAAAATATTGTCGATTTTTTGAAAAGTTTGGTCAGAAAAATGCTTAAAAAAATGAAAAATCAACCCCTACTTTTTGTGGAAGTTCTCTTCTGGAAAACTCGCAAAGAATGCCATTACATTAATGCTGAGTATTTATTGCAAGAGCTTGGCCATTTGAAGAAAGAAACTAGAAACTGGGCAAACAGTTTAGGGGATGACGAAATTGGTCAATCACTGGACAAAGGATGGACAAGTAGAAGCATAGCAGATGCACTTGGTGAAGATGAAGCTGACGTCGTTCTCCCTCATGACCTTGGATATGAGAATGATGGAGAAAATTCTGACAAAGCGAAGGGAGGTACTGCATCTATTTCAGATAATGAGATTGATGGGCAAGCTAATTATGACAATGAGGGAAAGTCCATAGAGAATGAAACTGAGAGGGTTTCTATTAAAAACAAGCGACTTGTTATTGGAGCTGAACTGGAGATGAAAATTAAGGATCTGTATGAGAAATTCAAGGATGACCAGAATTGTAGTCATCTTATTGCGGAAGCTCTTGATCCTGATGGTAAAGTTTCATCAGCTCAAATTTCCAACAAGCTTAAACAGCTGGGGCTGACAGTTGCACGAAGGAAAAGAATTCGGAAAGCTCAGGAATCTGTTTCCACTGGGCCTAGTCAAATTGGTGGAGATGGAAGAGTAGAGGAAGCAGTTAACACTCATTCTGAATCAAAAAGCCAGCCTTTGCGAGCAAGAAAAAGGGTCCATGCCCTCAGTGAAGATCAGGAAGCTAATATCAGAGCTTTGTATGAGCAATTTAAAGACCATAAGAAGTGCAGCCATATGATTGCAAATGCGATGGATGGAGATGGGAAATTCACAGCTCCTCAAGTTTCTCGTAAGCTTAAGCAGCTTGGTTTGCATATTCCTCGGAAGAAGAGGCCTTCAGGTGGCATGCTGAAGGATGAGGATCATAGCGACTCTATTGCAAACAAAATGCACGACTCAGATGATGAAACGCTGTTTTCATTAATGAAAAGGGGCAAAAAGGATGGTAGCAAGGAGTTGCTAGAACAGACTGCGGGACGAGGAACGTTGGAAGTTGATTCTGATGATGAAATCCTAAGCTCTGTTCTCAAGAAAACTAGGCGATCCCTTTCCAAGTCCATGGATCAAAATTCCGAAGCCATCTCGATTCAGGGAACAGAAAGCGGCAGTGTTTTGGAAAATGAAGTTGACGAAGGTGCTGCTGCCAAACGTGCTAGTCTGAATGGCGCAGAACAAGCTGAAGTCACAGGCACCATCAGTGGTAATGCATTAGATGTTGGACCTGTGAAAAATTTGGAGGATTTGCTACGTCAGGACATGGACACTGATTTGGCAGATTCGGAGGATGAAGTAGGTACATTGCCAGTGAGTGGTGTAAGCAGAAGGAGGTCAAGGATGGTAGTTGAAGATGAAGACGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

1324

Amino Acids

148.64

Weight (kDa)

5.5

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIMELESS PF04821 69 - 375 9.8e-56 Timeless protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1875, 2361
AatII GACGTC 1 cut(s) 2793
Acc36I ACCTGC 2 cut(s) 371, 2248
Acc65I GGTACC 2 cut(s) 110, 331
AccB1I GGYRCC 3 cut(s) 110, 331, 3797
AccB7I CCANNNNNTGG 1 cut(s) 3917
AccII CGCG 1 cut(s) 44
AciI CCGC 5 cut(s) 272, 1911, 3047, 3581, 3711
AclI AACGTT 1 cut(s) 3593
AclWI GGATC 9 cut(s) 206, 1248, 1475, 2420, 3006, 3053, 3477, 3645, 3678
AcoI YGGCCR 3 cut(s) 3, 735, 2665
AcuI CTGAAG 3 cut(s) 3429, 3479, 3807
AcyI GRCGYC 1 cut(s) 2790
AdeI CACNNNGTG 1 cut(s) 2774
AfaI GTAC 7 cut(s) 112, 221, 333, 669, 2380, 2857, 3904
AfiI CCNNNNNNNGG 5 cut(s) 120, 947, 1466, 3449, 3917
AflII CTTAAG 3 cut(s) 1700, 2351, 3404
AflIII ACRYGT 1 cut(s) 409
AhlI ACTAGT 1 cut(s) 1582
AjnI CCWGG 1 cut(s) 2424
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
Alw26I GTCTC 2 cut(s) 1034, 2150
AlwI GGATC 9 cut(s) 206, 1248, 1475, 2420, 3006, 3053, 3477, 3645, 3678
Ama87I CYCGRG 1 cut(s) 1212
AoxI GGCC 6 cut(s) 3, 735, 1166, 2665, 3170, 3440
ApeKI GCWGC 9 cut(s) 661, 715, 1553, 1652, 2264, 3336, 3409, 3746, 3749
ArsI GACNNNNNNTTYG 2 cut(s) 2511, 2543
AseI ATTAAT 2 cut(s) 2637, 3530
Asp700I GAANNNNTTC 2 cut(s) 975, 1899
Asp718I GGTACC 2 cut(s) 110, 331
AspLEI GCGC 2 cut(s) 46, 3776
AspS9I GGNCC 6 cut(s) 938, 1217, 1544, 3170, 3262, 3827
AsuC2I CCSGG 2 cut(s) 1213, 1214
AsuHPI GGTGA 5 cut(s) 842, 1123, 1544, 2228, 2786
AsuII TTCGAA 2 cut(s) 2407, 2517
AvaI CYCGRG 1 cut(s) 1212
AvaII GGWCC 5 cut(s) 938, 1217, 1544, 3262, 3827
AxyI CCTNAGG 1 cut(s) 942
BaeGI GKGCMC 1 cut(s) 1472
BalI TGGCCA 2 cut(s) 5, 2667
BanI GGYRCC 3 cut(s) 110, 331, 3797
BanII GRGCYC 1 cut(s) 2433
BbsI GAAGAC 5 cut(s) 546, 1390, 1437, 2112, 3966
BbvI GCAGC 9 cut(s) 673, 727, 1565, 1664, 2251, 3348, 3421, 3733, 3736
BceAI ACGGC 2 cut(s) 750, 1901
BciT130I CCWGG 1 cut(s) 2426
BclI TGATCA 2 cut(s) 1768, 2122
BcnI CCSGG 2 cut(s) 1213, 1214
BcoDI GTCTC 2 cut(s) 1034, 2150
BcuI ACTAGT 1 cut(s) 1582
BfmI CTRYAG 2 cut(s) 1488, 1650
BfrI CTTAAG 3 cut(s) 1700, 2351, 3404
BfuAI ACCTGC 2 cut(s) 371, 2248
BlpI GCTNAGC 1 cut(s) 2487
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 3 cut(s) 1213, 1214, 2426
Bme18I GGWCC 5 cut(s) 938, 1217, 1544, 3262, 3827
BmeT110I CYCGRG 1 cut(s) 1212
BmgT120I GGNCC 6 cut(s) 938, 1217, 1544, 3170, 3262, 3827
BmiI GGNNCC 7 cut(s) 112, 333, 1218, 1546, 1728, 3263, 3799
BmrFI CCNGG 3 cut(s) 1213, 1214, 2426
BmrI ACTGGG 2 cut(s) 2701, 3177
BmsI GCATC 8 cut(s) 619, 1323, 1498, 1565, 2037, 2334, 2755, 2870
BmuI ACTGGG 2 cut(s) 2701, 3177
BpiI GAAGAC 5 cut(s) 546, 1390, 1437, 2112, 3966
BpmI CTGGAG 3 cut(s) 56, 747, 3002
Bpu10I CCTNAGC 3 cut(s) 248, 3150, 3623
Bpu1102I GCTNAGC 1 cut(s) 2487
Bpu14I TTCGAA 2 cut(s) 2407, 2517
BpuEI CTTGAG 3 cut(s) 1016, 3372, 3620
BpuMI CCSGG 2 cut(s) 1213, 1214
Bsa29I ATCGAT 1 cut(s) 2187
BsaAI YACGTR 1 cut(s) 1085
BsaBI GATNNNNATC 2 cut(s) 2027, 3468
BsaHI GRCGYC 1 cut(s) 2790
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 1477, 1507
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 947, 1466, 3449, 3917
Bse21I CCTNAGG 1 cut(s) 942
Bse3DI GCAATG 3 cut(s) 1250, 1548, 3905
Bse8I GATNNNNATC 2 cut(s) 2027, 3468
BseBI CCWGG 1 cut(s) 2426
BseCI ATCGAT 1 cut(s) 2187
BseJI GATNNNNATC 2 cut(s) 2027, 3468
BseLI CCNNNNNNNGG 5 cut(s) 120, 947, 1466, 3449, 3917
BseMI GCAATG 3 cut(s) 1250, 1548, 3905
BseMII CTCAG 8 cut(s) 769, 1427, 2478, 2634, 2925, 3164, 3286, 3519
BseRI GAGGAG 4 cut(s) 51, 59, 2450, 3375
BseSI GKGCMC 1 cut(s) 1472
BseXI GCAGC 9 cut(s) 673, 727, 1565, 1664, 2251, 3348, 3421, 3733, 3736
BseYI CCCAGC 3 cut(s) 819, 1125, 3109
BsgI GTGCAG 2 cut(s) 680, 3355
Bsh1236I CGCG 1 cut(s) 44
Bsh1285I CGRYCG 2 cut(s) 1968, 2191
BshFI GGCC 6 cut(s) 5, 737, 1168, 2667, 3172, 3442
BshNI GGYRCC 3 cut(s) 110, 331, 3797
BshVI ATCGAT 1 cut(s) 2187
BsiEI CGRYCG 2 cut(s) 1968, 2191
BsiHKCI CYCGRG 1 cut(s) 1212
BsiSI CCGG 2 cut(s) 196, 1213
BslFI GGGAC 3 cut(s) 449, 1261, 3597
BslI CCNNNNNNNGG 5 cut(s) 120, 947, 1466, 3449, 3917
BsmAI GTCTC 2 cut(s) 1034, 2150
BsmFI GGGAC 3 cut(s) 449, 1261, 3597
BsmI GAATGC 4 cut(s) 382, 632, 2141, 2630
BsnI GGCC 6 cut(s) 5, 737, 1168, 2667, 3172, 3442
BsoBI CYCGRG 1 cut(s) 1212
Bsp119I TTCGAA 2 cut(s) 2407, 2517
Bsp1286I GDGCHC 2 cut(s) 1472, 2433
Bsp1720I GCTNAGC 1 cut(s) 2487
Bsp19I CCATGG 2 cut(s) 1134, 3666
BspACI CCGC 5 cut(s) 272, 1911, 3047, 3581, 3711
BspANI GGCC 6 cut(s) 5, 737, 1168, 2667, 3172, 3442
BspCNI CTCAG 8 cut(s) 768, 1426, 2479, 2635, 2926, 3163, 3285, 3518
BspDI ATCGAT 1 cut(s) 2187
BspFNI CGCG 1 cut(s) 44
BspHI TCATGA 3 cut(s) 784, 853, 2803
BspLI GGNNCC 7 cut(s) 112, 333, 1218, 1546, 1728, 3263, 3799
BspMAI CTGCAG 1 cut(s) 1654
BspMI ACCTGC 2 cut(s) 371, 2248
BspPI GGATC 9 cut(s) 206, 1248, 1475, 2420, 3006, 3053, 3477, 3645, 3678
BspQI GCTCTTC 1 cut(s) 2034
BspT104I TTCGAA 2 cut(s) 2407, 2517
BspT107I GGYRCC 3 cut(s) 110, 331, 3797
BspTI CTTAAG 3 cut(s) 1700, 2351, 3404
BsrDI GCAATG 3 cut(s) 1250, 1548, 3905
BssNI GRCGYC 1 cut(s) 2790
BssT1I CCWWGG 6 cut(s) 210, 980, 1134, 1722, 2809, 3666
Bst2UI CCWGG 1 cut(s) 2426
Bst4CI ACNGT 6 cut(s) 110, 115, 577, 1489, 2702, 3121
Bst6I CTCTTC 8 cut(s) 406, 961, 1053, 1376, 2034, 2609, 3190, 3431
BstACI GRCGYC 1 cut(s) 2790
BstAFI CTTAAG 3 cut(s) 1700, 2351, 3404
BstAPI GCANNNNNTGC 2 cut(s) 222, 2625
BstBAI YACGTR 1 cut(s) 1085
BstBI TTCGAA 2 cut(s) 2407, 2517
BstDSI CCRYGG 2 cut(s) 1134, 3666
BstFNI CGCG 1 cut(s) 44
BstHHI GCGC 2 cut(s) 46, 3776
BstMAI GTCTC 2 cut(s) 1034, 2150
BstMCI CGRYCG 2 cut(s) 1968, 2191
BstNI CCWGG 1 cut(s) 2426
BstNSI RCATGY 4 cut(s) 413, 1687, 2404, 3457
BstSCI CCNGG 3 cut(s) 1211, 1212, 2424
BstSFI CTRYAG 2 cut(s) 1488, 1650
BstSLI GKGCMC 1 cut(s) 1472
BstSNI TACGTA 1 cut(s) 1085
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 9 cut(s) 673, 727, 1565, 1664, 2251, 3348, 3421, 3733, 3736
BstV2I GAAGAC 5 cut(s) 546, 1390, 1437, 2112, 3966
BstX2I RGATCY 2 cut(s) 1480, 2998
BstXI CCANNNNNNTGG 2 cut(s) 827, 3667
BstYI RGATCY 2 cut(s) 1480, 2998
Bsu15I ATCGAT 1 cut(s) 2187
Bsu36I CCTNAGG 1 cut(s) 942
BsuRI GGCC 6 cut(s) 5, 737, 1168, 2667, 3172, 3442
BsuTUI ATCGAT 1 cut(s) 2187
BtgI CCRYGG 2 cut(s) 1134, 3666
BtgZI GCGATG 1 cut(s) 3371
BtsI GCAGTG 4 cut(s) 346, 471, 1008, 3721
BveI ACCTGC 2 cut(s) 371, 2248
CciI TCATGA 3 cut(s) 784, 853, 2803
CfoI GCGC 2 cut(s) 46, 3776
Cfr13I GGNCC 6 cut(s) 938, 1217, 1544, 3170, 3262, 3827
Cfr9I CCCGGG 1 cut(s) 1212
ClaI ATCGAT 1 cut(s) 2187
Csp6I GTAC 7 cut(s) 111, 220, 332, 668, 2379, 2856, 3903
CviQI GTAC 7 cut(s) 111, 220, 332, 668, 2379, 2856, 3903
DraI TTTAAA 1 cut(s) 3319
DraIII CACNNNGTG 1 cut(s) 2774
EaeI YGGCCR 3 cut(s) 3, 735, 2665
Eam1104I CTCTTC 8 cut(s) 406, 961, 1053, 1376, 2034, 2609, 3190, 3431
EarI CTCTTC 8 cut(s) 406, 961, 1053, 1376, 2034, 2609, 3190, 3431
EciI GGCGGA 1 cut(s) 1926
Eco105I TACGTA 1 cut(s) 1085
Eco130I CCWWGG 6 cut(s) 210, 980, 1134, 1722, 2809, 3666
Eco147I AGGCCT 2 cut(s) 1168, 3442
Eco24I GRGCYC 1 cut(s) 2433
Eco47I GGWCC 5 cut(s) 938, 1217, 1544, 3262, 3827
Eco57I CTGAAG 3 cut(s) 3429, 3479, 3807
Eco81I CCTNAGG 1 cut(s) 942
Eco88I CYCGRG 1 cut(s) 1212
EcoO109I RGGNCCY 1 cut(s) 938
EcoRI GAATTC 2 cut(s) 971, 3138
EcoRII CCWGG 1 cut(s) 2424
EcoT14I CCWWGG 6 cut(s) 210, 980, 1134, 1722, 2809, 3666
EcoT22I ATGCAT 3 cut(s) 634, 1609, 3817
EcoT38I GRGCYC 1 cut(s) 2433
ErhI CCWWGG 6 cut(s) 210, 980, 1134, 1722, 2809, 3666
FalI AAGNNNNNCTT 8 cut(s) 116, 148, 209, 241, 1156, 1188, 3226, 3258
FaqI GGGAC 3 cut(s) 449, 1261, 3597
FauI CCCGC 2 cut(s) 279, 3574
FauNDI CATATG 2 cut(s) 1609, 3342
FbaI TGATCA 2 cut(s) 1768, 2122
FriOI GRGCYC 1 cut(s) 2433
GlaI GCGC 2 cut(s) 45, 3775
GsaI CCCAGC 3 cut(s) 823, 1129, 3113
GsuI CTGGAG 3 cut(s) 56, 747, 3002
HaeIII GGCC 6 cut(s) 5, 737, 1168, 2667, 3172, 3442
HapII CCGG 2 cut(s) 196, 1213
HhaI GCGC 2 cut(s) 46, 3776
Hin1I GRCGYC 1 cut(s) 2790
Hin6I GCGC 2 cut(s) 44, 3774
HinP1I GCGC 2 cut(s) 44, 3774
HincII GTYRAC 3 cut(s) 2295, 3214, 3736
HindII GTYRAC 3 cut(s) 2295, 3214, 3736
HindIII AAGCTT 2 cut(s) 3098, 3401
HpaI GTTAAC 1 cut(s) 3214
HpaII CCGG 2 cut(s) 196, 1213
HphI GGTGA 5 cut(s) 842, 1123, 1544, 2228, 2786
Hpy166II GTNNAC 6 cut(s) 1300, 1544, 1564, 2295, 3214, 3736
Hpy8I GTNNAC 6 cut(s) 1300, 1544, 1564, 2295, 3214, 3736
Hpy99I CGWCG 2 cut(s) 511, 2795
HpyCH4III ACNGT 6 cut(s) 110, 115, 577, 1489, 2702, 3121
HpyCH4IV ACGT 8 cut(s) 423, 625, 792, 1084, 2790, 3593, 3757, 3856
HpySE526I ACGT 8 cut(s) 423, 625, 792, 1084, 2790, 3593, 3757, 3856
Hsp92I GRCGYC 1 cut(s) 2790
HspAI GCGC 2 cut(s) 44, 3774
KpnI GGTACC 2 cut(s) 114, 335
Ksp22I TGATCA 2 cut(s) 1768, 2122
KspAI GTTAAC 1 cut(s) 3214
LguI GCTCTTC 1 cut(s) 2034
LmnI GCTCC 5 cut(s) 1290, 1498, 1726, 2972, 3388
Lsp1109I GCAGC 9 cut(s) 673, 727, 1565, 1664, 2251, 3348, 3421, 3733, 3736
LweI GCATC 8 cut(s) 619, 1323, 1498, 1565, 2037, 2334, 2755, 2870
MaeII ACGT 8 cut(s) 423, 625, 792, 1084, 2790, 3593, 3757, 3856
MaeIII GTNAC 4 cut(s) 1085, 1319, 2062, 3790
MflI RGATCY 2 cut(s) 1480, 2998
MhlI GDGCHC 2 cut(s) 1472, 2433
MlsI TGGCCA 2 cut(s) 5, 2667
MluNI TGGCCA 2 cut(s) 5, 2667
MlyI GAGTC 4 cut(s) 1102, 1783, 3473, 3497
MmeI TCCRAC 6 cut(s) 115, 1508, 2133, 3117, 3576, 3805
Mox20I TGGCCA 2 cut(s) 5, 2667
Mph1103I ATGCAT 3 cut(s) 634, 1609, 3817
MroXI GAANNNNTTC 2 cut(s) 975, 1899
MscI TGGCCA 2 cut(s) 5, 2667
MslI CAYNNNNRTG 5 cut(s) 414, 828, 893, 1110, 2503
Msp20I TGGCCA 2 cut(s) 5, 2667
MspA1I CMGCKG 3 cut(s) 664, 1655, 3109
MspCI CTTAAG 3 cut(s) 1700, 2351, 3404
MspI CCGG 2 cut(s) 196, 1213
MspR9I CCNGG 3 cut(s) 1213, 1214, 2426
Mva1269I GAATGC 4 cut(s) 382, 632, 2141, 2630
MvaI CCWGG 1 cut(s) 2426
MvnI CGCG 1 cut(s) 44
NciI CCSGG 2 cut(s) 1213, 1214
NcoI CCATGG 2 cut(s) 1134, 3666
NdeI CATATG 2 cut(s) 1609, 3342
NlaIV GGNNCC 7 cut(s) 112, 333, 1218, 1546, 1728, 3263, 3799
NmuCI GTSAC 2 cut(s) 2062, 3790
NsiI ATGCAT 3 cut(s) 634, 1609, 3817
NspI RCATGY 4 cut(s) 413, 1687, 2404, 3457
NspV TTCGAA 2 cut(s) 2407, 2517
PaeI GCATGC 3 cut(s) 1687, 2404, 3457
PagI TCATGA 3 cut(s) 784, 853, 2803
PceI AGGCCT 2 cut(s) 1168, 3442
PciI ACATGT 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 2034
PctI GAATGC 4 cut(s) 382, 632, 2141, 2630
PdmI GAANNNNTTC 2 cut(s) 975, 1899
PflFI GACNNNGTC 1 cut(s) 2207
PflMI CCANNNNNTGG 1 cut(s) 3917
Ple19I CGATCG 2 cut(s) 1968, 2191
PleI GAGTC 4 cut(s) 1101, 1783, 3473, 3497
PpsI GAGTC 4 cut(s) 1101, 1783, 3473, 3497
Ppu21I YACGTR 1 cut(s) 1085
PpuMI RGGWCCY 1 cut(s) 938
PscI ACATGT 1 cut(s) 409
PshBI ATTAAT 2 cut(s) 2637, 3530
PsiI TTATAA 2 cut(s) 1875, 2361
Psp1406I AACGTT 1 cut(s) 3593
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 2424
PspFI CCCAGC 3 cut(s) 819, 1125, 3109
PspGI CCWGG 1 cut(s) 2424
PspN4I GGNNCC 7 cut(s) 112, 333, 1218, 1546, 1728, 3263, 3799
PspPI GGNCC 6 cut(s) 938, 1217, 1544, 3170, 3262, 3827
PspPPI RGGWCCY 1 cut(s) 938
PstI CTGCAG 1 cut(s) 1654
PsuI RGATCY 2 cut(s) 1480, 2998
PsyI GACNNNGTC 1 cut(s) 2207
PvuI CGATCG 2 cut(s) 1968, 2191
PvuII CAGCTG 3 cut(s) 664, 1655, 3109
RsaI GTAC 7 cut(s) 112, 221, 333, 669, 2380, 2857, 3904
RsaNI GTAC 7 cut(s) 111, 220, 332, 668, 2379, 2856, 3903
RseI CAYNNNNRTG 5 cut(s) 414, 828, 893, 1110, 2503
SapI GCTCTTC 1 cut(s) 2034
Sau96I GGNCC 6 cut(s) 938, 1217, 1544, 3170, 3262, 3827
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 4 cut(s) 1102, 1783, 3473, 3497
ScrFI CCNGG 3 cut(s) 1213, 1214, 2426
SduI GDGCHC 2 cut(s) 1472, 2433
SfaNI GCATC 8 cut(s) 619, 1323, 1498, 1565, 2037, 2334, 2755, 2870
SfcI CTRYAG 2 cut(s) 1488, 1650
SfuI TTCGAA 2 cut(s) 2407, 2517
SinI GGWCC 5 cut(s) 938, 1217, 1544, 3262, 3827
SmaI CCCGGG 1 cut(s) 1214
SmiMI CAYNNNNRTG 5 cut(s) 414, 828, 893, 1110, 2503
SmlI CTYRAG 6 cut(s) 1031, 1700, 2351, 3387, 3404, 3635
SmoI CTYRAG 6 cut(s) 1031, 1700, 2351, 3387, 3404, 3635
SnaBI TACGTA 1 cut(s) 1085
SpeI ACTAGT 1 cut(s) 1582
SphI GCATGC 3 cut(s) 1687, 2404, 3457
SseBI AGGCCT 2 cut(s) 1168, 3442
SsiI CCGC 5 cut(s) 272, 1911, 3047, 3581, 3711
SspI AATATT 3 cut(s) 313, 1354, 2524
StuI AGGCCT 2 cut(s) 1168, 3442
StyD4I CCNGG 3 cut(s) 1211, 1212, 2424
StyI CCWWGG 6 cut(s) 210, 980, 1134, 1722, 2809, 3666
TaaI ACNGT 6 cut(s) 110, 115, 577, 1489, 2702, 3121
TaiI ACGT 8 cut(s) 426, 628, 795, 1087, 2793, 3596, 3760, 3859
TaqI TCGA 8 cut(s) 509, 557, 1968, 2187, 2407, 2517, 2529, 3692
TatI WGTACW 2 cut(s) 219, 2378
TauI GCSGC 1 cut(s) 3714
TseFI GTSAC 2 cut(s) 2062, 3790
TseI GCWGC 9 cut(s) 661, 715, 1553, 1652, 2264, 3336, 3409, 3746, 3749
Tsp45I GTSAC 2 cut(s) 2062, 3790
TspGWI ACGGA 2 cut(s) 136, 653
TspMI CCCGGG 1 cut(s) 1212
Tth111I GACNNNGTC 1 cut(s) 2207
Van91I CCANNNNNTGG 1 cut(s) 3917
Vha464I CTTAAG 3 cut(s) 1700, 2351, 3404
VpaK11BI GGWCC 5 cut(s) 938, 1217, 1544, 3262, 3827
VspI ATTAAT 2 cut(s) 2637, 3530
XbaI TCTAGA 2 cut(s) 769, 1076
XceI RCATGY 4 cut(s) 413, 1687, 2404, 3457
XcmI CCANNNNNNNNNTGG 1 cut(s) 1462
XmaI CCCGGG 1 cut(s) 1212
XmnI GAANNNNTTC 2 cut(s) 975, 1899
ZraI GACGTC 1 cut(s) 2791
ZrmI AGTACT 1 cut(s) 221
Zsp2I ATGCAT 3 cut(s) 634, 1609, 3817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.