pycom02g01130

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014543
Physical Location & Seq
Reverse (-)
627036 .. 630387
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3006 bp
ATGTTTTCTCACTCGACTCTCACACAAAAACAGACTTCCATAATATCTGACACATTTTTCTTCCAATCGGGCATCCAAAATTGTCTGTTCTCTCAGTCATCCCAACTGCAAGTTCCAATGGAGATTTTCCATTCCTCCTTCTACTTAATCCTTCTCCTCCTCTTCGCGGCGGTTTCCGCCGCCGATATCTACTCAGACACCCTTCTCAGCTTAAAATCCGAGCTCACAGACGCCAATGGCAGCTTGAAAGATTGGATTCTCCCTTCTACAGAAAACCCACCTGGCAAAATCCACGCATGTTCTTGGTCCGGCGTCAAATGCAACACCAACAACTCCGCCGTCACTGGCTTAGACCTCTCAATAAAGAGTCTCGCCGGTGAAATGTCCGGCGATCAATTCGCCGTCTTCGCCGACCTTGTATCACTCAATCTCAGTCAAAACTCGTTCTCCGGTCACCTCCCCGCTGGAATCTTCAACCTCACCAACCTCACAACATTAGACATCAGCAGAAACAACTTCTCCGGCCACTTCCCCACCGGCGTCTCCACCCTCCAAAACCTCATTGTACTCGACGCATTCAGCAACAGCTTTTCCGGGTCGCTCCCCGCCGAAATTTCAGACCTTCAGAACCTCAAAATCCTCAATTTAGCCGGAAGCTACTTCCGGGGACCGATCCCGTCGGAATACGGGGCTTTTCGCCGGCTCGAGTTTATTCATCTCGCCGGGAATATGATCACCGGAAGCATTCCGCCGGAACTGGGGAAGCTCAAAACAGTGACCCACATGGAGATTGGCTACAATTTTTACCACGGAAGCATTCCGTGGCAATTGGGGAACATGACAGAGCTCCAGTATCTCGACATTGCCTATGCAAATCTCTCCGGCGCCGTCCCAAAATCCCTCGGCGATCTCGACAATCTCGAATCCCTCTTCCTTTTCAGAAACCAGCTTTCTGGGTCAATTCCGCCTGAGCTCAGCAACATCAGAGCTCTCCAAAGCTTGGATCTTTCGGACAATCTGATTTCTGGGTGGATCCCAGAAACCTTTTCGGAGCTGAAAAACCTTAGACTCCTCAGTCTTTTTTACAACGAGATGAGCGGCGAAGTTCCTGACGGAATTGCAGAGCTTCCGTCTTTGGAGACGCTGCTTTTGTGGAACAATTTCTTCTCCGGCAACCTTCCCCGGAGCTTGGGGAGGAACTCGAAGCTCGGATGGGTGGACGTCTCCACAAACAACTTCAACGGCAGCGTTCCGCCGGATATCTGTGCGAATGGTCAGCTCCTTAAGCTGATGCTGTTTTCGAACAATTTCTCCGGCGATCTCTCCACTTCTCTGTCAAACTGTTCGTCTCTGGTTCGTCTTCGACTCGAAGATAACAAGTTTTCAGGTGAGATTCCATTGAAATTCAGCACTCTGACTGACATCACATATGTTGATTTATCTGGGAACAATTTTAGTGGTGGGATTCCAATGGATATTTCAAAAGCTCAAAAGCTTGAGTACTTCAATGTCTCTAGAAATCCAGAATTAGGGGGCACAGTTCCTGCAGAAATGTTGGAATTGCCAAATCTTCACAATTTTTCGGCTTCCTCGTGTGGCTTGATTGGCAATGTTCCTTCGTTCGAAAACTGCAAATCAATATCTGTTGTTGAATTGAGCAAGAACAGCTTGGAAGGAACTGTCCCAAAAAGTATTTCTAGCTGCCGGGTTCTTGAGAGGATTGATTTAGGTAGCAATAATATATCCGGTCGTATACCCAAGGAGCTTGCAAGTCTCCCTGCTCTTCTTGTTCTTGACCTGTCACATAACAGCTTCAATGGACCAATACCTAAAACTTTTGGTTCTTCTTCGAGTTTAGTACTTCTAAATGTGTCTTACAATGATGTTTCCGGGAGTATTCCATCCGAAAAGGTATTTAGAATGATGGAACGAAGCGCTTTTGATGGGAATCCAAAGCTGTGTGGAGAGCCTCTCAGATCATGTCCTGGCTCAGTGTCCATATTTGGAAGCAGAGTTGCCGGGAAGCTTCTGTGGATCGCACTACTCTGCATCGGAGTAGTTATGTTCGTTACTCTATCAGTTATGGGGATATTTTACTACCATAAAGGCAGCAAGGGCCAGTGGAAGATGATCTCATTTGCTGGACTTCCTCAATTCACAAAAAATGATTTATTGAAGAGCTTCAGTTCGACAGAATCCATGGATGCATTGCCACCTTTGTCAGCTTCAGTGCGGAAAGCAGTTCTGCCAATGGGAATTGCAGTTTCGGTAAAGAAGATTGAATGGGGAGCAAAGAGAATGAGAGTTATGCTGGAATTTGTAACACAAATAGGAAATGCAAGGCATAAGAATTTAATTCGATTGCTTGGAGTTTGCTACAACAACCATCTGGCTTATCTCTTATACGATTACTTGCCCAATGGAAACTTGGCTGAAATGATAAGTGTGAAAAGCGAATGGGCAGCCAAGTACAAAATTATCATCGACATTGCAAAGGGATTATGCTTCCTTCACCATGAATGTTATCCTGCAATAGCACATGGAGATCTGAGATCAAGTAATATAGTGTTTGACGAAAATATGGAACCTCATTTGACAGAATTCGGGTTCAAGCATCTGCTCGAGTTGAGCAAGGGTTCGTTTACAGCAGCAACTTCCACCTGGGATGCAGGTGATAACATCAGTGGCACAAAAGAGGAGCTATGCAGGGATGTATACAGCTTTGGGGAGATCATGCTGGAGATTCTAAGCAACGGCAGGTTGACAAATTCTGGCGCAAGCATACAGAGCAAGTCGAGGGAAGTTGTTCTGCGGGAAATTTACAACGAGAACGAAGCTGGTTCCGACATTTCAATGCAAGAGGAGATAAAACTGGCAATTGAGGTTGCTATGCTTTGCACCAGGAGTAGGCCATCCGATCGACCATCCATGGAAAATGCATTGAAGCTTTTATCCGAGTCGAAACTCCAGATGAAAAATAACCCAACTTTTGAAACTGCAGGGTGA

Protein Analysis

1002

Amino Acids

109.62

Weight (kDa)

5.68

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 199 - 315 1.5e-08 Leucine-rich repeat region
LRR_14 PF23598 319 - 387 5.9e-06 Leucine-rich repeat region
LRR_8 PF13855 330 - 387 1.8e-08 Leucine rich repeat
LRR_8 PF13855 571 - 628 1.1e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 752 - 981 7.8e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 755 - 982 2.2e-21 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51350
fragaria_vesca FvH4_1g01520
malus_domestica MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica Prupe.7G253000_v2.0.a1
pyrus_communis pycom02g01130 pycom15g14160
rosa_chinensis RchiOBHm_Chr2g0086441
rosa_laevigata RLG00000015759
rosa_multiflora Rmu_sc0005127.1_g000003
rosa_roxburghii Rroxscaffold_2G00154570
rosa_rugosa Rorug01G0467300
rosa_samantha Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana Rw2G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2660
AasI GACNNNNNNGTC 1 cut(s) 1074
AatII GACGTC 1 cut(s) 1224
Acc36I ACCTGC 2 cut(s) 2660, 2748
AccB1I GGYRCC 1 cut(s) 884
AccB7I CCANNNNNTGG 1 cut(s) 1000
AccBSI CCGCTC 1 cut(s) 1098
AccI GTMKAC 2 cut(s) 1753, 2715
AccII CGCG 1 cut(s) 167
AclWI GGATC 5 cut(s) 667, 1011, 1027, 1040, 2042
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 7 cut(s) 612, 1403, 2315, 2350, 2600, 2767, 2817
AcuI CTGAAG 3 cut(s) 608, 2167, 2211
AcyI GRCGYC 5 cut(s) 231, 312, 540, 885, 1221
AfaI GTAC 4 cut(s) 567, 1502, 1860, 2471
AfeI AGCGCT 1 cut(s) 1936
AfiI CCNNNNNNNGG 6 cut(s) 166, 758, 1000, 1189, 1529, 2907
AflII CTTAAG 1 cut(s) 1283
AjnI CCWGG 4 cut(s) 280, 1984, 2660, 2900
AjuI GAANNNNNNNTTGG 6 cut(s) 96, 128, 1462, 1494, 1678, 1710
AloI GAACNNNNNNTCC 2 cut(s) 1295, 1327
Alw21I GWGCWC 4 cut(s) 225, 849, 975, 991
Alw26I GTCTC 7 cut(s) 374, 547, 1133, 1228, 1353, 1516, 1778
AlwI GGATC 5 cut(s) 667, 1011, 1027, 1040, 2042
AlwNI CAGNNNCTG 1 cut(s) 1544
Ama87I CYCGRG 2 cut(s) 704, 2621
Aor51HI AGCGCT 1 cut(s) 1936
AoxI GGCC 3 cut(s) 523, 2116, 2909
ApeKI GCWGC 7 cut(s) 240, 1144, 1245, 1701, 2109, 2462, 2648
ApoI RAATTY 7 cut(s) 612, 1403, 2315, 2350, 2600, 2767, 2817
ArsI GACNNNNNNTTYG 4 cut(s) 1132, 1164, 1331, 1363
Asp700I GAANNNNTTC 4 cut(s) 1160, 1307, 2180, 2805
AspLEI GCGC 3 cut(s) 887, 1937, 2777
AspS9I GGNCC 4 cut(s) 306, 668, 1820, 2116
AsuC2I CCSGG 7 cut(s) 595, 665, 724, 1183, 1706, 1891, 2020
AsuHPI GGTGA 7 cut(s) 389, 446, 472, 727, 1400, 2504, 2684
AsuII TTCGAA 2 cut(s) 1301, 1623
AvaI CYCGRG 2 cut(s) 704, 2621
AvaII GGWCC 3 cut(s) 306, 668, 1820
BaeGI GKGCMC 1 cut(s) 1538
BamHI GGATCC 1 cut(s) 1032
BanI GGYRCC 1 cut(s) 884
BanII GRGCYC 4 cut(s) 225, 849, 975, 991
BauI CACGAG 1 cut(s) 1591
BbsI GAAGAC 2 cut(s) 397, 1352
Bbv12I GWGCWC 4 cut(s) 225, 849, 975, 991
BbvI GCAGC 7 cut(s) 252, 1131, 1257, 1688, 2121, 2474, 2660
BccI CCATC 7 cut(s) 1206, 1909, 1918, 1937, 2394, 2920, 2932
BceAI ACGGC 5 cut(s) 323, 386, 872, 1258, 2770
BciT130I CCWGG 4 cut(s) 282, 1986, 2662, 2902
BclI TGATCA 1 cut(s) 732
BcnI CCSGG 7 cut(s) 595, 665, 724, 1183, 1706, 1891, 2020
BcoDI GTCTC 7 cut(s) 374, 547, 1133, 1228, 1353, 1516, 1778
BfaI CTAG 2 cut(s) 1515, 1698
BfmI CTRYAG 3 cut(s) 267, 1545, 2997
BfoI RGCGCY 2 cut(s) 888, 1938
BfrI CTTAAG 1 cut(s) 1283
BfuAI ACCTGC 2 cut(s) 2660, 2748
BglII AGATCT 1 cut(s) 2545
BlpI GCTNAGC 1 cut(s) 974
BmcAI AGTACT 2 cut(s) 1502, 1860
Bme18I GGWCC 3 cut(s) 306, 668, 1820
BmeT110I CYCGRG 2 cut(s) 704, 2621
BmgT120I GGNCC 4 cut(s) 306, 668, 1820, 2116
BmiI GGNNCC 5 cut(s) 669, 886, 1034, 2586, 2842
BmrI ACTGGG 1 cut(s) 767
BmsI GCATC 6 cut(s) 81, 1281, 2058, 2194, 2623, 2656
BmuI ACTGGG 1 cut(s) 767
BpiI GAAGAC 2 cut(s) 397, 1352
BplI GAGNNNNNCTC 2 cut(s) 1956, 1988
BpmI CTGGAG 3 cut(s) 833, 2759, 2951
Bpu10I CCTNAGC 1 cut(s) 969
Bpu1102I GCTNAGC 1 cut(s) 974
Bpu14I TTCGAA 2 cut(s) 1301, 1623
BpuEI CTTGAG 2 cut(s) 1517, 1733
BpuMI CCSGG 7 cut(s) 595, 665, 724, 1183, 1706, 1891, 2020
BsaBI GATNNNNATC 1 cut(s) 1947
BsaHI GRCGYC 5 cut(s) 231, 312, 540, 885, 1221
BsaJI CCNNGG 9 cut(s) 664, 808, 821, 901, 1181, 1758, 2199, 2661, 2928
BsaWI WCCGGW 3 cut(s) 449, 737, 1745
BsaXI ACNNNNNCTCC 6 cut(s) 431, 461, 503, 533, 1295, 1325
Bsc4I CCNNNNNNNGG 6 cut(s) 166, 758, 1000, 1189, 1529, 2907
Bse118I RCCGGY 3 cut(s) 374, 536, 699
Bse1I ACTGG 5 cut(s) 349, 762, 850, 2119, 2877
Bse3DI GCAATG 4 cut(s) 861, 1615, 2207, 2487
Bse8I GATNNNNATC 1 cut(s) 1947
BseBI CCWGG 4 cut(s) 282, 1986, 2662, 2902
BseDI CCNNGG 9 cut(s) 664, 808, 821, 901, 1181, 1758, 2199, 2661, 2928
BseGI GGATG 9 cut(s) 72, 98, 1217, 1901, 2209, 2671, 2716, 2912, 2924
BseJI GATNNNNATC 1 cut(s) 1947
BseLI CCNNNNNNNGG 6 cut(s) 166, 758, 1000, 1189, 1529, 2907
BseMI GCAATG 4 cut(s) 861, 1615, 2207, 2487
BseNI ACTGG 5 cut(s) 349, 762, 850, 2119, 2877
BseRI GAGGAG 5 cut(s) 146, 149, 1061, 2711, 2876
BseSI GKGCMC 1 cut(s) 1538
BseXI GCAGC 7 cut(s) 252, 1131, 1257, 1688, 2121, 2474, 2660
Bsh1236I CGCG 1 cut(s) 167
Bsh1285I CGRYCG 2 cut(s) 1750, 2920
BshFI GGCC 3 cut(s) 525, 2118, 2911
BshNI GGYRCC 1 cut(s) 884
BsiEI CGRYCG 2 cut(s) 1750, 2920
BsiHKAI GWGCWC 4 cut(s) 225, 849, 975, 991
BsiHKCI CYCGRG 2 cut(s) 704, 2621
BslFI GGGAC 3 cut(s) 681, 875, 1667
BslI CCNNNNNNNGG 6 cut(s) 166, 758, 1000, 1189, 1529, 2907
BsmAI GTCTC 7 cut(s) 374, 547, 1133, 1228, 1353, 1516, 1778
BsmBI CGTCTC 4 cut(s) 547, 1133, 1228, 1353
BsmFI GGGAC 3 cut(s) 681, 875, 1667
BsmI GAATGC 3 cut(s) 575, 744, 816
BsnI GGCC 3 cut(s) 525, 2118, 2911
BsoBI CYCGRG 2 cut(s) 704, 2621
Bsp119I TTCGAA 2 cut(s) 1301, 1623
Bsp1286I GDGCHC 5 cut(s) 225, 849, 975, 991, 1538
Bsp1720I GCTNAGC 1 cut(s) 974
Bsp19I CCATGG 2 cut(s) 2199, 2928
BspANI GGCC 3 cut(s) 525, 2118, 2911
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 5 cut(s) 669, 886, 1034, 2586, 2842
BspMAI CTGCAG 2 cut(s) 1549, 3001
BspMI ACCTGC 2 cut(s) 2660, 2748
BspPI GGATC 5 cut(s) 667, 1011, 1027, 1040, 2042
BspQI GCTCTTC 2 cut(s) 1788, 2171
BspT104I TTCGAA 2 cut(s) 1301, 1623
BspT107I GGYRCC 1 cut(s) 884
BspTI CTTAAG 1 cut(s) 1283
BsrBI CCGCTC 1 cut(s) 1098
BsrDI GCAATG 4 cut(s) 861, 1615, 2207, 2487
BsrFI RCCGGY 3 cut(s) 374, 536, 699
BsrI ACTGG 5 cut(s) 349, 762, 850, 2119, 2877
BssAI RCCGGY 3 cut(s) 374, 536, 699
BssECI CCNNGG 9 cut(s) 664, 808, 821, 901, 1181, 1758, 2199, 2661, 2928
BssNAI GTATAC 2 cut(s) 1754, 2716
BssNI GRCGYC 5 cut(s) 231, 312, 540, 885, 1221
BssSI CACGAG 1 cut(s) 1591
BssT1I CCWWGG 3 cut(s) 1758, 2199, 2928
Bst1107I GTATAC 2 cut(s) 1754, 2716
Bst2BI CACGAG 1 cut(s) 1591
Bst2UI CCWGG 4 cut(s) 282, 1986, 2662, 2902
Bst4CI ACNGT 4 cut(s) 775, 1343, 1540, 1681
Bst6I CTCTTC 4 cut(s) 167, 935, 1788, 2171
BstACI GRCGYC 5 cut(s) 231, 312, 540, 885, 1221
BstAFI CTTAAG 1 cut(s) 1283
BstBI TTCGAA 2 cut(s) 1301, 1623
BstC8I GCNNGC 3 cut(s) 701, 1767, 2779
BstDSI CCRYGG 4 cut(s) 808, 821, 2199, 2928
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 9 cut(s) 72, 98, 1217, 1901, 2209, 2671, 2716, 2912, 2924
BstFNI CGCG 1 cut(s) 167
BstH2I RGCGCY 2 cut(s) 888, 1938
BstHHI GCGC 3 cut(s) 887, 1937, 2777
BstMAI GTCTC 7 cut(s) 374, 547, 1133, 1228, 1353, 1516, 1778
BstMCI CGRYCG 2 cut(s) 1750, 2920
BstMWI GCNNNNNNNGC 8 cut(s) 176, 318, 407, 1285, 1605, 1665, 2115, 2787
BstNI CCWGG 4 cut(s) 282, 1986, 2662, 2902
BstNSI RCATGY 1 cut(s) 300
BstPI GGTNACC 1 cut(s) 452
BstSFI CTRYAG 3 cut(s) 267, 1545, 2997
BstSLI GKGCMC 1 cut(s) 1538
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 7 cut(s) 252, 1131, 1257, 1688, 2121, 2474, 2660
BstV2I GAAGAC 2 cut(s) 397, 1352
BstX2I RGATCY 3 cut(s) 1003, 1032, 2545
BstXI CCANNNNNNTGG 1 cut(s) 953
BstYI RGATCY 3 cut(s) 1003, 1032, 2545
BstZ17I GTATAC 2 cut(s) 1754, 2716
BsuRI GGCC 3 cut(s) 525, 2118, 2911
BtgI CCRYGG 4 cut(s) 808, 821, 2199, 2928
BtsCI GGATG 9 cut(s) 72, 98, 1217, 1901, 2209, 2671, 2716, 2912, 2924
BtsIMutI CAGTG 6 cut(s) 342, 780, 1998, 2126, 2235, 2689
BveI ACCTGC 2 cut(s) 2660, 2748
Cac8I GCNNGC 3 cut(s) 701, 1767, 2779
CaiI CAGNNNCTG 1 cut(s) 1544
CfoI GCGC 3 cut(s) 887, 1937, 2777
Cfr10I RCCGGY 3 cut(s) 374, 536, 699
Cfr13I GGNCC 4 cut(s) 306, 668, 1820, 2116
CseI GACGC 5 cut(s) 239, 301, 529, 581, 1150
Csp6I GTAC 4 cut(s) 566, 1501, 1859, 2470
CspCI CAANNNNNGTGG 2 cut(s) 1438, 1473
CviAII CATG 9 cut(s) 297, 784, 838, 1980, 2200, 2516, 2540, 2734, 2929
CviQI GTAC 4 cut(s) 566, 1501, 1859, 2470
DinI GGCGCC 1 cut(s) 886
DrdI GACNNNNNNGTC 1 cut(s) 1074
DseDI GACNNNNNNGTC 1 cut(s) 1074
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 4 cut(s) 167, 935, 1788, 2171
EarI CTCTTC 4 cut(s) 167, 935, 1788, 2171
EciI GGCGGA 5 cut(s) 166, 325, 738, 954, 1242
Ecl136II GAGCTC 4 cut(s) 223, 847, 973, 989
Eco130I CCWWGG 3 cut(s) 1758, 2199, 2928
Eco24I GRGCYC 4 cut(s) 225, 849, 975, 991
Eco32I GATATC 2 cut(s) 187, 1261
Eco47I GGWCC 3 cut(s) 306, 668, 1820
Eco47III AGCGCT 1 cut(s) 1936
Eco53kI GAGCTC 4 cut(s) 223, 847, 973, 989
Eco57I CTGAAG 3 cut(s) 608, 2167, 2211
Eco88I CYCGRG 2 cut(s) 704, 2621
Eco91I GGTNACC 1 cut(s) 452
EcoICRI GAGCTC 4 cut(s) 223, 847, 973, 989
EcoO65I GGTNACC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 2600
EcoRII CCWGG 4 cut(s) 280, 1984, 2660, 2900
EcoRV GATATC 2 cut(s) 187, 1261
EcoT14I CCWWGG 3 cut(s) 1758, 2199, 2928
EcoT22I ATGCAT 2 cut(s) 2209, 2941
EcoT38I GRGCYC 4 cut(s) 225, 849, 975, 991
EgeI GGCGCC 1 cut(s) 886
EheI GGCGCC 1 cut(s) 886
ErhI CCWWGG 3 cut(s) 1758, 2199, 2928
Esp3I CGTCTC 4 cut(s) 547, 1133, 1228, 1353
FaeI CATG 9 cut(s) 300, 787, 841, 1983, 2203, 2519, 2543, 2737, 2932
FalI AAGNNNNNCTT 2 cut(s) 1652, 1684
FaqI GGGAC 3 cut(s) 681, 875, 1667
FatI CATG 9 cut(s) 296, 783, 837, 1979, 2199, 2515, 2539, 2733, 2928
FauI CCCGC 3 cut(s) 469, 613, 2805
FauNDI CATATG 1 cut(s) 1429
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 2 cut(s) 1753, 2715
FokI GGATG 9 cut(s) 59, 85, 1224, 1888, 2216, 2678, 2723, 2899, 2911
FriOI GRGCYC 4 cut(s) 225, 849, 975, 991
FspBI CTAG 2 cut(s) 1515, 1698
GlaI GCGC 3 cut(s) 886, 1936, 2776
GsuI CTGGAG 3 cut(s) 833, 2759, 2951
HaeII RGCGCY 2 cut(s) 888, 1938
HaeIII GGCC 3 cut(s) 525, 2118, 2911
HgaI GACGC 5 cut(s) 239, 301, 529, 581, 1150
HhaI GCGC 3 cut(s) 887, 1937, 2777
Hin1I GRCGYC 5 cut(s) 231, 312, 540, 885, 1221
Hin1II CATG 9 cut(s) 300, 787, 841, 1983, 2203, 2519, 2543, 2737, 2932
Hin6I GCGC 3 cut(s) 885, 1935, 2775
HinP1I GCGC 3 cut(s) 885, 1935, 2775
HincII GTYRAC 1 cut(s) 2763
HindII GTYRAC 1 cut(s) 2763
HindIII AAGCTT 4 cut(s) 997, 1493, 2024, 2945
HphI GGTGA 7 cut(s) 389, 446, 472, 727, 1400, 2504, 2684
Hpy166II GTNNAC 5 cut(s) 1219, 1754, 2643, 2716, 2763
Hpy188III TCNNGA 9 cut(s) 857, 911, 920, 1109, 1515, 1523, 1712, 1793, 2968
Hpy8I GTNNAC 5 cut(s) 1219, 1754, 2643, 2716, 2763
Hpy99I CGWCG 2 cut(s) 575, 682
HpyAV CCTTC 9 cut(s) 148, 161, 212, 273, 632, 1187, 1626, 1667, 2519
HpyCH4III ACNGT 4 cut(s) 775, 1343, 1540, 1681
HpyCH4IV ACGT 1 cut(s) 1221
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 318, 407, 1285, 1605, 1665, 2115, 2787
HpySE526I ACGT 1 cut(s) 1221
Hsp92I GRCGYC 5 cut(s) 231, 312, 540, 885, 1221
Hsp92II CATG 9 cut(s) 300, 787, 841, 1983, 2203, 2519, 2543, 2737, 2932
HspAI GCGC 3 cut(s) 885, 1935, 2775
KasI GGCGCC 1 cut(s) 884
KroI GCCGGC 1 cut(s) 699
KroNI GCCGGC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 732
LguI GCTCTTC 2 cut(s) 1788, 2171
LmnI GCTCC 8 cut(s) 606, 852, 1051, 1185, 1284, 1762, 2288, 2698
Lsp1109I GCAGC 7 cut(s) 252, 1131, 1257, 1688, 2121, 2474, 2660
LweI GCATC 6 cut(s) 81, 1281, 2058, 2194, 2623, 2656
MaeI CTAG 2 cut(s) 1515, 1698
MaeII ACGT 1 cut(s) 1221
MaeIII GTNAC 6 cut(s) 340, 452, 775, 1800, 2068, 2320
MbiI CCGCTC 1 cut(s) 1098
MfeI CAATTG 2 cut(s) 827, 2877
MflI RGATCY 3 cut(s) 1003, 1032, 2545
MhlI GDGCHC 5 cut(s) 225, 849, 975, 991, 1538
Mly113I GGCGCC 1 cut(s) 885
MlyI GAGTC 5 cut(s) 10, 376, 1062, 1359, 2966
MmeI TCCRAC 3 cut(s) 660, 1536, 2868
Mph1103I ATGCAT 2 cut(s) 2209, 2941
MroNI GCCGGC 1 cut(s) 699
MroXI GAANNNNTTC 4 cut(s) 1160, 1307, 2180, 2805
MseI TTAA 4 cut(s) 146, 212, 1284, 2354
MslI CAYNNNNRTG 1 cut(s) 2852
MspA1I CMGCKG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 1283
MunI CAATTG 2 cut(s) 827, 2877
Mva1269I GAATGC 3 cut(s) 575, 744, 816
MvaI CCWGG 4 cut(s) 282, 1986, 2662, 2902
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 8 cut(s) 176, 318, 407, 1285, 1605, 1665, 2115, 2787
NaeI GCCGGC 1 cut(s) 701
NarI GGCGCC 1 cut(s) 885
NciI CCSGG 7 cut(s) 595, 665, 724, 1183, 1706, 1891, 2020
NcoI CCATGG 2 cut(s) 2199, 2928
NdeI CATATG 1 cut(s) 1429
NgoMIV GCCGGC 1 cut(s) 699
NlaIII CATG 9 cut(s) 300, 787, 841, 1983, 2203, 2519, 2543, 2737, 2932
NlaIV GGNNCC 5 cut(s) 669, 886, 1034, 2586, 2842
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 4 cut(s) 340, 452, 775, 1800
NsiI ATGCAT 2 cut(s) 2209, 2941
NspI RCATGY 1 cut(s) 300
NspV TTCGAA 2 cut(s) 1301, 1623
PaeR7I CTCGAG 2 cut(s) 704, 2621
PaqCI CACCTGC 1 cut(s) 2660
PciSI GCTCTTC 2 cut(s) 1788, 2171
PcsI WCGNNNNNNNCGW 3 cut(s) 909, 918, 1589
PctI GAATGC 3 cut(s) 575, 744, 816
PdiI GCCGGC 1 cut(s) 701
PdmI GAANNNNTTC 4 cut(s) 1160, 1307, 2180, 2805
PfeI GAWTC 8 cut(s) 256, 468, 923, 1393, 1465, 1948, 2195, 2743
PflMI CCANNNNNTGG 1 cut(s) 1000
PfoI TCCNGGA 1 cut(s) 1889
Ple19I CGATCG 1 cut(s) 2920
PleI GAGTC 5 cut(s) 10, 375, 1062, 1359, 2965
PluTI GGCGCC 1 cut(s) 888
PpsI GAGTC 5 cut(s) 10, 375, 1062, 1359, 2965
Psp124BI GAGCTC 4 cut(s) 225, 849, 975, 991
Psp6I CCWGG 4 cut(s) 280, 1984, 2660, 2900
PspEI GGTNACC 1 cut(s) 452
PspGI CCWGG 4 cut(s) 280, 1984, 2660, 2900
PspN4I GGNNCC 5 cut(s) 669, 886, 1034, 2586, 2842
PspPI GGNCC 4 cut(s) 306, 668, 1820, 2116
PspXI VCTCGAGB 2 cut(s) 704, 2621
PstI CTGCAG 2 cut(s) 1549, 3001
PstNI CAGNNNCTG 1 cut(s) 1544
PsuI RGATCY 3 cut(s) 1003, 1032, 2545
PvuI CGATCG 1 cut(s) 2920
RsaI GTAC 4 cut(s) 567, 1502, 1860, 2471
RsaNI GTAC 4 cut(s) 566, 1501, 1859, 2470
RseI CAYNNNNRTG 1 cut(s) 2852
SacI GAGCTC 4 cut(s) 225, 849, 975, 991
SapI GCTCTTC 2 cut(s) 1788, 2171
SaqAI TTAA 4 cut(s) 146, 212, 1284, 2354
Sau96I GGNCC 4 cut(s) 306, 668, 1820, 2116
ScaI AGTACT 2 cut(s) 1502, 1860
SchI GAGTC 5 cut(s) 10, 376, 1062, 1359, 2966
SduI GDGCHC 5 cut(s) 225, 849, 975, 991, 1538
SfaNI GCATC 6 cut(s) 81, 1281, 2058, 2194, 2623, 2656
SfcI CTRYAG 3 cut(s) 267, 1545, 2997
SfoI GGCGCC 1 cut(s) 886
Sfr274I CTCGAG 2 cut(s) 704, 2621
SfuI TTCGAA 2 cut(s) 1301, 1623
SgrAI CRCCGGYG 2 cut(s) 374, 536
SinI GGWCC 3 cut(s) 306, 668, 1820
SlaI CTCGAG 2 cut(s) 704, 2621
SmiMI CAYNNNNRTG 1 cut(s) 2852
SmlI CTYRAG 5 cut(s) 704, 1283, 1496, 1712, 2621
SmoI CTYRAG 5 cut(s) 704, 1283, 1496, 1712, 2621
SspDI GGCGCC 1 cut(s) 884
SspMI CTAG 2 cut(s) 1515, 1698
SstI GAGCTC 4 cut(s) 225, 849, 975, 991
StyI CCWWGG 3 cut(s) 1758, 2199, 2928
TaaI ACNGT 4 cut(s) 775, 1343, 1540, 1681
TaiI ACGT 1 cut(s) 1224
TaqII GACCGA 1 cut(s) 685
TatI WGTACW 4 cut(s) 565, 1500, 1858, 2469
TauI GCSGC 3 cut(s) 170, 182, 1101
TfiI GAWTC 8 cut(s) 256, 468, 923, 1393, 1465, 1948, 2195, 2743
Tru1I TTAA 4 cut(s) 146, 212, 1284, 2354
Tru9I TTAA 4 cut(s) 146, 212, 1284, 2354
TscAI CASTG 6 cut(s) 349, 780, 1998, 2126, 2235, 2689
TseFI GTSAC 4 cut(s) 340, 452, 775, 1800
TseI GCWGC 7 cut(s) 240, 1144, 1245, 1701, 2109, 2462, 2648
Tsp45I GTSAC 4 cut(s) 340, 452, 775, 1800
TspDTI ATGAA 3 cut(s) 704, 2532, 2987
TspGWI ACGGA 4 cut(s) 810, 825, 1119, 1128
TspRI CASTG 6 cut(s) 349, 780, 1998, 2126, 2235, 2689
Van91I CCANNNNNTGG 1 cut(s) 1000
Vha464I CTTAAG 1 cut(s) 1283
VpaK11BI GGWCC 3 cut(s) 306, 668, 1820
XapI RAATTY 7 cut(s) 612, 1403, 2315, 2350, 2600, 2767, 2817
XbaI TCTAGA 1 cut(s) 1514
XceI RCATGY 1 cut(s) 300
XcmI CCANNNNNNNNNTGG 2 cut(s) 788, 2425
XhoI CTCGAG 2 cut(s) 704, 2621
XmiI GTMKAC 2 cut(s) 1753, 2715
XmnI GAANNNNTTC 4 cut(s) 1160, 1307, 2180, 2805
XspI CTAG 2 cut(s) 1515, 1698
ZraI GACGTC 1 cut(s) 1222
ZrmI AGTACT 2 cut(s) 1502, 1860
Zsp2I ATGCAT 2 cut(s) 2209, 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.