pycom02g05670

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005500
Physical Location & Seq
Reverse (-)
3846332 .. 3847654
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGCAAGGCGAATTGTGTTTCGAGGATCCGTCAACTTTCTCCATTTTGATTGTCGGGAATATTGATCCCTCTATAGCACTTCCTCTTATATTGCAGTATATGGGTGGGATACCAAAGGCTCCTGAACCGCTCCTACAATTTAATCGCGATGACCTCGTAGGACTACCATTCACCTTTCCTAAAACCAGAATAAGAGAAGTGGTTCGCAGCCCAATGGTGGAAGAACAATGTTCTGTCCAATTGTGCTTCCCTGTGGAACTGAAAAATGGAACCATGCTGTGTACCTTTCTTGTCGTATATTACAAAGGAAAAACATTTATATGGCAGGTAGAAGACATTCACATTGTTGGATTCCTGAGCAAACTTCTTGAAACAAAAATTATGCAGGTTCCACGATTCAAACATGGACAGATCTACACTGTAGGTGTTTCTGTATTTCTTGGGGGTAGTAAGCCTTCCAGAACTGCAAATGTTCGTGGTGATCGAATCATAAACTTTTCTTGTGATCCAGAAATCTCGTCAAAGCTGGTTGGTGCTTTTGTTGTCGATCTTACTCTGGATGAAATAATACATCTCCAAGAGGAAGGGCCGTCAGATGAGGATGTCTCAACCATCCTTGAAATTGAGCAAAGAGCCCACGAAAATGGGCTTCAAGTAAGTTTTCTTGTTACAAAAGGTTTCATTTTGGTCAGTGATGCACCATTGTTCCAACAATCACCTAGTGCACAAAGTTTCGTGGATGCGAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

251

Amino Acids

27.88

Weight (kDa)

4.91

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 10 - 205 7.2e-14 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 316, 376
AccBSI CCGCTC 1 cut(s) 130
AccII CGCG 1 cut(s) 147
AciI CCGC 1 cut(s) 128
AclWI GGATC 4 cut(s) 20, 33, 59, 500
AdeI CACNNNGTG 1 cut(s) 722
AfaI GTAC 1 cut(s) 283
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 4 cut(s) 371, 400, 620, 653
AluBI AGCT 1 cut(s) 526
AluI AGCT 1 cut(s) 526
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 2 cut(s) 610, 741
Alw44I GTGCAC 1 cut(s) 723
AlwI GGATC 4 cut(s) 20, 33, 59, 500
AoxI GGCC 1 cut(s) 587
ApaLI GTGCAC 1 cut(s) 723
ApeKI GCWGC 1 cut(s) 207
Asp700I GAANNNNTTC 2 cut(s) 201, 336
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 3 cut(s) 163, 491, 708
BaeGI GKGCMC 1 cut(s) 727
BamHI GGATCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 637
BarI GAAGNNNNNNTAC 2 cut(s) 439, 471
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 1 cut(s) 219
BccI CCATC 1 cut(s) 620
BceAI ACGGC 1 cut(s) 574
BciVI GTATCC 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 610, 741
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 2 cut(s) 72, 420
BfuAI ACCTGC 2 cut(s) 316, 376
BfuI GTATCC 1 cut(s) 102
BglII AGATCT 1 cut(s) 411
BisI GCNGC 1 cut(s) 208
BlsI GCNGC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 4 cut(s) 27, 120, 271, 390
BmsI GCATC 2 cut(s) 685, 730
BpiI GAAGAC 1 cut(s) 339
BplI GAGNNNNNCTC 2 cut(s) 590, 622
Bpu10I CCTNAGC 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 217
BseGI GGATG 4 cut(s) 565, 607, 612, 745
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 1 cut(s) 219
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 727
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 610, 741
BsnI GGCC 1 cut(s) 589
Bsp1286I GDGCHC 2 cut(s) 637, 727
Bsp143I GATC 6 cut(s) 25, 64, 411, 481, 505, 547
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 589
BspCNI CTCAG 1 cut(s) 348
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 4 cut(s) 27, 120, 271, 390
BspMI ACCTGC 2 cut(s) 316, 376
BspPI GGATC 4 cut(s) 20, 33, 59, 500
BsrBI CCGCTC 1 cut(s) 130
BssMI GATC 6 cut(s) 25, 64, 411, 481, 505, 547
Bst4CI ACNGT 1 cut(s) 421
BstDEI CTNAG 1 cut(s) 356
BstF5I GGATG 4 cut(s) 565, 607, 612, 745
BstFNI CGCG 1 cut(s) 147
BstKTI GATC 6 cut(s) 28, 67, 414, 484, 508, 550
BstMAI GTCTC 2 cut(s) 610, 741
BstMBI GATC 6 cut(s) 25, 64, 411, 481, 505, 547
BstSFI CTRYAG 2 cut(s) 72, 420
BstSLI GKGCMC 1 cut(s) 727
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 219
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 2 cut(s) 25, 411
BstXI CCANNNNNNTGG 1 cut(s) 644
BstYI RGATCY 2 cut(s) 25, 411
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 1 cut(s) 589
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 4 cut(s) 565, 607, 612, 745
BtsIMutI CAGTG 2 cut(s) 417, 697
BtuMI TCGCGA 1 cut(s) 147
BveI ACCTGC 2 cut(s) 316, 376
Cfr13I GGNCC 1 cut(s) 587
Csp6I GTAC 1 cut(s) 282
CviAII CATG 2 cut(s) 274, 404
CviJI RGCY 7 cut(s) 119, 210, 454, 526, 589, 635, 649
CviKI_1 RGCY 7 cut(s) 119, 210, 454, 526, 589, 635, 649
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 1 cut(s) 356
DpnI GATC 6 cut(s) 27, 66, 413, 483, 507, 549
DpnII GATC 6 cut(s) 25, 64, 411, 481, 505, 547
DraIII CACNNNGTG 1 cut(s) 722
Eco24I GRGCYC 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 637
FaeI CATG 2 cut(s) 277, 407
FatI CATG 2 cut(s) 273, 403
Fnu4HI GCNGC 1 cut(s) 208
FokI GGATG 3 cut(s) 572, 599, 614
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 1 cut(s) 208
FspBI CTAG 1 cut(s) 720
GluI GCNGC 1 cut(s) 208
HaeIII GGCC 1 cut(s) 589
Hin1II CATG 2 cut(s) 277, 407
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 3 cut(s) 351, 396, 486
HphI GGTGA 3 cut(s) 163, 491, 708
Hpy166II GTNNAC 3 cut(s) 33, 282, 725
Hpy188I TCNGA 1 cut(s) 595
Hpy188III TCNNGA 8 cut(s) 55, 122, 146, 355, 368, 459, 509, 557
Hpy8I GTNNAC 3 cut(s) 33, 282, 725
HpyAV CCTTC 2 cut(s) 465, 578
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4V TGCA 6 cut(s) 4, 94, 385, 467, 698, 725
HpyF3I CTNAG 1 cut(s) 356
Hsp92II CATG 2 cut(s) 277, 407
Kzo9I GATC 6 cut(s) 25, 64, 411, 481, 505, 547
LmnI GCTCC 2 cut(s) 124, 135
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 2 cut(s) 685, 730
MaeI CTAG 1 cut(s) 720
MaeIII GTNAC 1 cut(s) 667
MalI GATC 6 cut(s) 27, 66, 413, 483, 507, 549
MbiI CCGCTC 1 cut(s) 130
MboI GATC 6 cut(s) 25, 64, 411, 481, 505, 547
MboII GAAGA 2 cut(s) 233, 344
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 2 cut(s) 25, 411
MhlI GDGCHC 2 cut(s) 637, 727
MluCI AATT 5 cut(s) 11, 137, 239, 378, 621
MmeI TCCRAC 2 cut(s) 328, 733
MnlI CCTC 6 cut(s) 16, 79, 93, 164, 574, 592
MroXI GAANNNNTTC 2 cut(s) 201, 336
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 2 cut(s) 319, 642
MunI CAATTG 1 cut(s) 239
MvnI CGCG 1 cut(s) 147
NdeII GATC 6 cut(s) 25, 64, 411, 481, 505, 547
NlaIII CATG 2 cut(s) 277, 407
NlaIV GGNNCC 4 cut(s) 27, 120, 271, 390
NruI TCGCGA 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PdmI GAANNNNTTC 2 cut(s) 201, 336
PfeI GAWTC 3 cut(s) 351, 396, 486
PkrI GCNGC 1 cut(s) 209
PspN4I GGNNCC 4 cut(s) 27, 120, 271, 390
PspPI GGNCC 1 cut(s) 587
PsrI GAACNNNNNNTAC 2 cut(s) 117, 149
PsuI RGATCY 2 cut(s) 25, 411
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
RseI CAYNNNNRTG 2 cut(s) 319, 642
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 1 cut(s) 208
Sau3AI GATC 6 cut(s) 25, 64, 411, 481, 505, 547
Sau96I GGNCC 1 cut(s) 587
SduI GDGCHC 2 cut(s) 637, 727
SetI ASST 9 cut(s) 156, 176, 287, 330, 390, 427, 528, 679, 721
SfaNI GCATC 2 cut(s) 685, 730
SfcI CTRYAG 2 cut(s) 72, 420
SmiMI CAYNNNNRTG 2 cut(s) 319, 642
Sse9I AATT 5 cut(s) 11, 137, 239, 378, 621
SsiI CCGC 1 cut(s) 128
SspI AATATT 1 cut(s) 61
SspMI CTAG 1 cut(s) 720
TaaI ACNGT 1 cut(s) 421
TaqI TCGA 3 cut(s) 21, 484, 546
TasI AATT 5 cut(s) 11, 137, 239, 378, 621
TfiI GAWTC 3 cut(s) 351, 396, 486
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 2 cut(s) 424, 697
TseI GCWGC 1 cut(s) 207
TspDTI ATGAA 2 cut(s) 576, 670
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 2 cut(s) 424, 697
VneI GTGCAC 1 cut(s) 723
XmnI GAANNNNTTC 2 cut(s) 201, 336
XspI CTAG 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.