RLG00000016323

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005500
Physical Location & Seq
Reverse (-)
5673361 .. 5682492
9132 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016323

Sequence Viewer

Length: 3390 bp
ATGGATTTACTTCCGGGAGGGAGTTCAAACATGGTAAAGAAAACCGGGTTCAGGTCACTGAAGCTGGTGAACATAGAGATGGACCAAGTTCTGGGAGACAAGCCCGTCGGAGTTGACTATGGCAGGCTTGATAATGGTCTCTCTTACTATGTTCGTTGCAATTCTAAGCCCAAAATGAGAGCAGCTCTTGCCCTTGCTGTTAAAGTCGGGTCAGTTTTGGAAGAGGAGGAAGAACGAGGAGTTGCCCACATTGTTGAGCATCTTGCTTTCAGTGCTACTCAGAAGTACACAAATCATGATATAGTCAGATTTCTTGAAAGTATTGGGGCAGAATTTGGCGCTTGCCAGAATGCAGTGACATCGGCTGATGACACTGTTTATGAGTTATTTGTTCCTGTTGACAAGCTTGAACTATTATCTGAGGCCATCTCTGTCTTGGCGGAATTCAGTTCTGAGATTCGTGTCTCAAAAGATGACGTGGAAAGAGAAAGAGGCGCTGTTATGGAAGAGTACAGAGGGAATCGAAATGCTACGGGAAGGATGCAGGATGCGCATTGGCTTTTAATGATGGCTGGTTCAAAGTATGCTGAGCGTTTACCTATTGGATTAGAAAAGGTAATTCGAACTGTTTCTCCTGAGACTGTGAAGCAATTTTATCAGAAGTGGTACCATTTAAGCAATATGGCAGTGATAGCTGTAGGAGATTTCCCGGATACTGAGAGTATAGTTGAGTTGATAAAGAATCAATTTGGGGATAAAAGTTCTGCACCTGAGCGTCCACTTATACCAACATATCCAGTTCCATCTCATGAAGAGCCACGTTATTCTTGTTTCATTGAATCTGAGGCAACTGGGTCTGCAGTGATTATTAGCTATAAGACTCCAGCTGTTGAGCTGAATACAGTGAGGGACTATAGGGATTTACTGGCAGAATCAATGTTCCTTTATGCTCTAAACCAGAGGTTCTTTAAAATAGCTCGTAGAAAAGATCCACCCTTTTTCTCATGCTCAACTTCCGCAGATGTTCTAGTTAATCCCTTGAAGGCTTATATGATGACCTCATCTTGTAAAGAAAAGGGAACAGTTCAGGCCCTAGAGTCGATGCTGATTGAGGTTGCCAGGGTACGGCTACATGGGTTTTCAGAACGGGAAGTGTCTATAGTTCGAGCTTTACTGATGTCAGAGATTGAATCTGCATATTTGGAACGCGATCAAATGCAGTCAACTAGCTTGCGAGATGAATATTTACAGCATTTTCTCCGTAATGAACCTGTTATTGGGATTGAGTATGAGGCTCAACTCCAGAAGACCCTTCTACCTTATATAACAGCAGCAGAGATATCCAAATATGCTGAGAAGTTACAAACATCATGCAGCTGTGTCATTAAGACAATTGAACCACGAGCTTCTGCAATAGTAGAAGATTTGAAAAATGTTGTCTCTAAAATCAGTGCTCTTGAGGAAGAAAGAATCATTTCTCCATGGGATGAAGAACATATTCCAGAAGAAATTGTTAGCACAAAACCTAATCCAGGAGTTTGCTATAAGAGTACAGACTTTCTTGATGATCAGGTTATCTTTACAGGGTTCTCATATGGGGGTTTATCCGAACTTGTTGAAAGTGAGTACTTTTCTTGCTCGATGGGGCCAACTATTGCCGGAGAAATTGGGGTATATGGTTATAGACCTTCAGTTCTGATGGATATGCTTGCGGGTAAGAGGTCAGAAGTCAGCACAAAGCTTGGAGCATACACGAGAACCTTTGCTGGTGATTGTTCACCCTCAGATTTAGAAACTGCTTTGCAGCTTGTGTATCAACTATTTACCACAAATGTAACACCAGGAGAAGAAGATGTCAAAATAGTGATGCAAATGGCAGAAGAAATGGTCCGTAATCAAGACAGGGATCCTTATACTGCATTTGCAAATCGTGTGAAGGAGCTGAACTATGGAAACTCCTATTTCTTTAGGCCTACCAGAATAAGTGATCTCCAGAAAGTTGATCCAATGAAAGCTTGTGAATATTTCAACAAATGTTTCAAGGATCCATCAACCTTCTCTATGGTCATTGTTGGGAATATTGATCCTTCTATAGCACTTCCTTTAATATTACAGTATTTGGGCGGAATACCAAAGCCTTCCGAACCTCTCATGCAATATAATCGCGATGACCTCACAGGACTGCCATTCACTTTTCCTAGAACCATAATAAGAGAAGTGGTGCGCAGCCCTATGGTGGAAGAACAATGTTCTGTCCAGCTTTGCTTTCCTGTGGAGCTGAATAATGGAACCATGGTAGAAGATATTCACGTTGTTGGTTTCCTGAGCAAACTTCTTGAAACGAAAATAATGCAAGTTCTCCGGTTCAAGCATGGACAGATCTACACTGTGGGTGTTTCAGTATTTCTTGGCGGTAATAAGCCTTCCAGAACTGCTAATGTTCGTGGAGATATAAGCATAAATTTTTCTTGTGATCCAGAAATTTCCTCGAAGCTGGTCGATCTTACTTTGGATGAAATATTACGTCTTCAAGAGGAAGGGCCTTCAGCTGAGGATGTCTCAACCATCCTTGAAATTGAGCAAAGAGCCCATGAAAATGGGATTCAGGAAAATTACTACTGGCTGGAGAGGATTTTACACAGCTACCAGTCAAGGATTTACTCTGGCGATGTTGGCACTTGTTTTGAGACTCAAGAGGAAGGTCGGTTAAAAGTTAGACAATCTCTGACCCCAAGAACGGCGCAGTTATCATTACAAAGCATACTACCTTATCCTTGCAAAAAACAGTACACTGTAGTAATCTTAATGCCCCGTACTTCTCGGTTTAAGTTACTAGATTCAATCTTTCGATCTACGACCAGTTTTGGTAGAGATGCTAAGATTTTAGCCGCGGTTGCTGGTCTCGCAGTTTTGGGTCTTAGCTTGTGGAGAGGGTTTAGCCTCTCTTGTTATTGCTCTCGCTTCCGCCTCTCTCTCTCATTCTGCGCATCGCTTCAAGGTAGAGTTGAAGAAGAGGAGAGTGTGAAAATGGTTGAGAAGACTAAGGGACGAAAGGAGGAGGTCGTCACCAGGGAGTTCACCATCAACCTCCACAAGCGCCTCCATGGCTGCACATTCAAGAAGAAGGCTCCCAATGCCATCAAGGAGATCAGGAAGTTTGCCCAGAAGGCAATGGGAACAATTGATGTCAGGGTGGATGTGAAGCTGAACAAGCAAATCTGGAGCAGAGGAATCAGGAGTGTCCCAAGAAGAATCAGGGTTCGTATTGCTCGCAAGAGGAATGATGATGAAGATGCAAAGGAAGAGCTATACTCCCTTGTGACTGTTGCAGAGATCCCAGCTGAAGGACTGAAGGGCTTGGGCACCAAGGTCATTGAAGAGGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

1130

Amino Acids

127.34

Weight (kDa)

5.72

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 54 - 172 5.5e-24 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 210 - 390 2.7e-17 Peptidase M16 inactive domain
Peptidase_M16_C PF05193 673 - 856 2.9e-19 Peptidase M16 inactive domain
Ribosomal_L31e PF01198 1020 - 1101 2.1e-40 Ribosomal protein L31e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
Acc16I TGCGCA 3 cut(s) 552, 2225, 2986
Acc65I GGTACC 1 cut(s) 666
AccB1I GGYRCC 2 cut(s) 666, 3362
AccB7I CCANNNNNTGG 1 cut(s) 91
AccII CGCG 3 cut(s) 1209, 2166, 2891
AciI CCGC 8 cut(s) 440, 1017, 1712, 2124, 2412, 2889, 2891, 2965
AclWI GGATC 9 cut(s) 983, 1901, 1914, 1997, 2039, 2052, 2078, 2468, 3328
AcsI RAATTY 4 cut(s) 332, 443, 2461, 2481
AcuI CTGAAG 5 cut(s) 80, 1674, 2529, 3363, 3371
AfaI GTAC 8 cut(s) 287, 512, 668, 1125, 1552, 1628, 2789, 2815
AfiI CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2236, 2737, 3344
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 4 cut(s) 1118, 1531, 1840, 3068
AjuI GAANNNNNNNTTGG 2 cut(s) 1260, 1292
AloI GAACNNNNNNTCC 2 cut(s) 616, 648
Alw21I GWGCWC 1 cut(s) 1456
Alw26I GTCTC 8 cut(s) 90, 143, 469, 632, 1444, 2563, 2681, 2906
AlwI GGATC 9 cut(s) 983, 1901, 1914, 1997, 2039, 2052, 2078, 2468, 3328
AoxI GGCC 5 cut(s) 423, 1089, 1646, 1970, 2540
ApeKI GCWGC 6 cut(s) 182, 1331, 1374, 1804, 2226, 3108
ApoI RAATTY 4 cut(s) 332, 443, 2461, 2481
Asp700I GAANNNNTTC 4 cut(s) 628, 1474, 1497, 2304
Asp718I GGTACC 1 cut(s) 666
AspLEI GCGC 7 cut(s) 341, 497, 553, 2226, 2743, 2987, 3099
AspS9I GGNCC 5 cut(s) 82, 1090, 1646, 1888, 2540
AsuC2I CCSGG 3 cut(s) 15, 46, 710
AsuHPI GGTGA 5 cut(s) 79, 1770, 1781, 3058, 3070
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 2 cut(s) 82, 1888
BaeGI GKGCMC 1 cut(s) 3365
BamHI GGATCC 2 cut(s) 1906, 2044
BanI GGYRCC 2 cut(s) 666, 3362
BanII GRGCYC 1 cut(s) 2590
BarI GAAGNNNNNNTAC 2 cut(s) 2407, 2439
BauI CACGAG 2 cut(s) 1401, 1753
BbsI GAAGAC 3 cut(s) 1313, 2519, 3044
Bbv12I GWGCWC 1 cut(s) 1456
BbvCI CCTCAGC 1 cut(s) 2550
BbvI GCAGC 6 cut(s) 194, 1343, 1386, 1816, 2238, 3095
BceAI ACGGC 2 cut(s) 1142, 2754
BcgI CGANNNNNNTGC 6 cut(s) 342, 376, 2144, 2178, 2332, 2366
BciT130I CCWGG 4 cut(s) 1120, 1533, 1842, 3070
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 1 cut(s) 1567
BcnI CCSGG 3 cut(s) 15, 46, 710
BcoDI GTCTC 8 cut(s) 90, 143, 469, 632, 1444, 2563, 2681, 2906
BfaI CTAG 5 cut(s) 1028, 1094, 1227, 2199, 2834
BfmI CTRYAG 6 cut(s) 696, 858, 913, 1158, 2091, 2793
BfoI RGCGCY 3 cut(s) 342, 498, 3100
BfuI GTATCC 1 cut(s) 706
BglI GCCNNNNNGGC 2 cut(s) 3105, 3167
BglII AGATCT 1 cut(s) 2379
BisI GCNGC 7 cut(s) 183, 1332, 1375, 1805, 2227, 2889, 3109
BlpI GCTNAGC 1 cut(s) 588
BlsI GCNGC 7 cut(s) 184, 1333, 1376, 1806, 2228, 2890, 3110
BmcAI AGTACT 1 cut(s) 1628
Bme1390I CCNGG 7 cut(s) 15, 46, 710, 1120, 1533, 1842, 3070
Bme18I GGWCC 2 cut(s) 82, 1888
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 5 cut(s) 82, 1090, 1646, 1888, 2540
BmiI GGNNCC 7 cut(s) 668, 1647, 1908, 2046, 2290, 3129, 3364
BmrFI CCNGG 7 cut(s) 15, 46, 710, 1120, 1533, 1842, 3070
BmrI ACTGGG 1 cut(s) 861
BmsI GCATC 8 cut(s) 268, 531, 538, 1092, 1857, 2863, 2996, 3283
BmuI ACTGGG 1 cut(s) 861
BpiI GAAGAC 3 cut(s) 1313, 2519, 3044
BplI GAGNNNNNCTC 8 cut(s) 169, 201, 413, 445, 2543, 2575, 3296, 3328
BpmI CTGGAG 5 cut(s) 867, 1286, 1976, 2645, 3241
Bpu10I CCTNAGC 3 cut(s) 771, 2324, 2550
Bpu1102I GCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 2 cut(s) 1478, 2676
BpuMI CCSGG 3 cut(s) 15, 46, 710
BsaI GGTCTC 2 cut(s) 143, 2906
BsaJI CCNNGG 7 cut(s) 1119, 1481, 2292, 2889, 3069, 3103, 3366
BsaWI WCCGGW 1 cut(s) 2361
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2236, 2737, 3344
Bse1I ACTGG 6 cut(s) 797, 856, 930, 2624, 2647, 2859
Bse3DI GCAATG 1 cut(s) 3177
BseBI CCWGG 4 cut(s) 1120, 1533, 1842, 3070
BseDI CCNNGG 7 cut(s) 1119, 1481, 2292, 2889, 3069, 3103, 3366
BseGI GGATG 8 cut(s) 546, 553, 1492, 2518, 2560, 2565, 3202, 3388
BseLI CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2236, 2737, 3344
BseMI GCAATG 1 cut(s) 3177
BseNI ACTGG 6 cut(s) 797, 856, 930, 2624, 2647, 2859
BseRI GAGGAG 4 cut(s) 239, 252, 3029, 3071
BseSI GKGCMC 1 cut(s) 3365
BseXI GCAGC 6 cut(s) 194, 1343, 1386, 1816, 2238, 3095
BseYI CCCAGC 1 cut(s) 3337
BsgI GTGCAG 2 cut(s) 750, 3094
Bsh1236I CGCG 3 cut(s) 1209, 2166, 2891
BshFI GGCC 5 cut(s) 425, 1091, 1648, 1972, 2542
BshNI GGYRCC 2 cut(s) 666, 3362
BsiHKAI GWGCWC 1 cut(s) 1456
BsiSI CCGG 5 cut(s) 14, 45, 710, 1659, 2362
BslFI GGGAC 3 cut(s) 923, 3060, 3227
BslI CCNNNNNNNGG 8 cut(s) 51, 91, 1125, 1277, 1532, 2236, 2737, 3344
BsmAI GTCTC 8 cut(s) 90, 143, 469, 632, 1444, 2563, 2681, 2906
BsmFI GGGAC 3 cut(s) 923, 3060, 3227
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 5 cut(s) 425, 1091, 1648, 1972, 2542
Bso31I GGTCTC 2 cut(s) 143, 2906
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 3 cut(s) 1456, 2590, 3365
Bsp1720I GCTNAGC 1 cut(s) 588
Bsp19I CCATGG 3 cut(s) 1481, 2292, 3103
Bsp68I TCGCGA 1 cut(s) 2166
BspACI CCGC 8 cut(s) 440, 1017, 1712, 2124, 2412, 2889, 2891, 2965
BspANI GGCC 5 cut(s) 425, 1091, 1648, 1972, 2542
BspFNI CGCG 3 cut(s) 1209, 2166, 2891
BspHI TCATGA 2 cut(s) 295, 808
BspLI GGNNCC 7 cut(s) 668, 1647, 1908, 2046, 2290, 3129, 3364
BspMAI CTGCAG 1 cut(s) 862
BspPI GGATC 9 cut(s) 983, 1901, 1914, 1997, 2039, 2052, 2078, 2468, 3328
BspQI GCTCTTC 2 cut(s) 807, 3297
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 2 cut(s) 666, 3362
BspTNI GGTCTC 2 cut(s) 143, 2906
BsrDI GCAATG 1 cut(s) 3177
BsrI ACTGG 6 cut(s) 797, 856, 930, 2624, 2647, 2859
BssECI CCNNGG 7 cut(s) 1119, 1481, 2292, 2889, 3069, 3103, 3366
BssSI CACGAG 2 cut(s) 1401, 1753
BssT1I CCWWGG 4 cut(s) 1481, 2292, 3103, 3366
Bst2BI CACGAG 2 cut(s) 1401, 1753
Bst2UI CCWGG 4 cut(s) 1120, 1533, 1842, 3070
Bst6I CTCTTC 6 cut(s) 216, 501, 807, 3006, 3297, 3372
BstAPI GCANNNNNTGC 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 5 cut(s) 125, 343, 1232, 1710, 3271
BstDSI CCRYGG 4 cut(s) 1481, 2292, 2889, 3103
BstENI CCTNNNNNAGG 1 cut(s) 1530
BstF5I GGATG 8 cut(s) 546, 553, 1492, 2518, 2560, 2565, 3202, 3388
BstFNI CGCG 3 cut(s) 1209, 2166, 2891
BstH2I RGCGCY 3 cut(s) 342, 498, 3100
BstHHI GCGC 7 cut(s) 341, 497, 553, 2226, 2743, 2987, 3099
BstMAI GTCTC 8 cut(s) 90, 143, 469, 632, 1444, 2563, 2681, 2906
BstNI CCWGG 4 cut(s) 1120, 1533, 1842, 3070
BstSCI CCNGG 7 cut(s) 13, 44, 708, 1118, 1531, 1840, 3068
BstSFI CTRYAG 6 cut(s) 696, 858, 913, 1158, 2091, 2793
BstSLI GKGCMC 1 cut(s) 3365
BstUI CGCG 3 cut(s) 1209, 2166, 2891
BstV1I GCAGC 6 cut(s) 194, 1343, 1386, 1816, 2238, 3095
BstV2I GAAGAC 3 cut(s) 1313, 2519, 3044
BstX2I RGATCY 5 cut(s) 988, 1906, 2044, 2379, 3333
BstXI CCANNNNNNTGG 1 cut(s) 2597
BstYI RGATCY 5 cut(s) 988, 1906, 2044, 2379, 3333
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 5 cut(s) 425, 1091, 1648, 1972, 2542
BtgI CCRYGG 4 cut(s) 1481, 2292, 2889, 3103
BtgZI GCGATG 3 cut(s) 2181, 2682, 2973
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 8 cut(s) 546, 553, 1492, 2518, 2560, 2565, 3202, 3388
BtsI GCAGTG 3 cut(s) 360, 693, 867
BtuMI TCGCGA 1 cut(s) 2166
Cac8I GCNNGC 5 cut(s) 125, 343, 1232, 1710, 3271
CciI TCATGA 2 cut(s) 295, 808
CfoI GCGC 7 cut(s) 341, 497, 553, 2226, 2743, 2987, 3099
Cfr13I GGNCC 5 cut(s) 82, 1090, 1646, 1888, 2540
Cfr42I CCGCGG 1 cut(s) 2892
CseI GACGC 1 cut(s) 764
Csp6I GTAC 8 cut(s) 286, 511, 667, 1124, 1551, 1627, 2788, 2814
CviQI GTAC 8 cut(s) 286, 511, 667, 1124, 1551, 1627, 2788, 2814
DraI TTTAAA 1 cut(s) 970
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
Eam1104I CTCTTC 6 cut(s) 216, 501, 807, 3006, 3297, 3372
EarI CTCTTC 6 cut(s) 216, 501, 807, 3006, 3297, 3372
EciI GGCGGA 3 cut(s) 455, 2139, 2954
Eco130I CCWWGG 4 cut(s) 1481, 2292, 3103, 3366
Eco147I AGGCCT 1 cut(s) 1972
Eco24I GRGCYC 1 cut(s) 2590
Eco31I GGTCTC 2 cut(s) 143, 2906
Eco32I GATATC 1 cut(s) 1341
Eco47I GGWCC 2 cut(s) 82, 1888
Eco57I CTGAAG 5 cut(s) 80, 1674, 2529, 3363, 3371
EcoNI CCTNNNNNAGG 1 cut(s) 1530
EcoO109I RGGNCCY 2 cut(s) 1090, 2540
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 4 cut(s) 1118, 1531, 1840, 3068
EcoRV GATATC 1 cut(s) 1341
EcoT14I CCWWGG 4 cut(s) 1481, 2292, 3103, 3366
EcoT38I GRGCYC 1 cut(s) 2590
ErhI CCWWGG 4 cut(s) 1481, 2292, 3103, 3366
FaqI GGGAC 3 cut(s) 923, 3060, 3227
FauI CCCGC 1 cut(s) 1705
FauNDI CATATG 1 cut(s) 1594
FbaI TGATCA 1 cut(s) 1567
Fnu4HI GCNGC 7 cut(s) 183, 1332, 1375, 1805, 2227, 2889, 3109
FokI GGATG 7 cut(s) 553, 560, 1499, 2525, 2552, 2567, 3209
FriOI GRGCYC 1 cut(s) 2590
Fsp4HI GCNGC 7 cut(s) 183, 1332, 1375, 1805, 2227, 2889, 3109
FspAI RTGCGCAY 1 cut(s) 552
FspBI CTAG 5 cut(s) 1028, 1094, 1227, 2199, 2834
FspI TGCGCA 3 cut(s) 552, 2225, 2986
GlaI GCGC 7 cut(s) 340, 496, 552, 2225, 2742, 2986, 3098
GluI GCNGC 7 cut(s) 183, 1332, 1375, 1805, 2227, 2889, 3109
GsaI CCCAGC 1 cut(s) 3341
GsuI CTGGAG 5 cut(s) 867, 1286, 1976, 2645, 3241
HaeII RGCGCY 3 cut(s) 342, 498, 3100
HaeIII GGCC 5 cut(s) 425, 1091, 1648, 1972, 2542
HapII CCGG 5 cut(s) 14, 45, 710, 1659, 2362
HgaI GACGC 1 cut(s) 764
HhaI GCGC 7 cut(s) 341, 497, 553, 2226, 2743, 2987, 3099
Hin6I GCGC 7 cut(s) 339, 495, 551, 2224, 2741, 2985, 3097
HinP1I GCGC 7 cut(s) 339, 495, 551, 2224, 2741, 2985, 3097
HincII GTYRAC 3 cut(s) 115, 400, 1224
HindII GTYRAC 3 cut(s) 115, 400, 1224
HindIII AAGCTT 3 cut(s) 404, 1739, 2013
HpaII CCGG 5 cut(s) 14, 45, 710, 1659, 2362
HphI GGTGA 5 cut(s) 79, 1770, 1781, 3058, 3070
Hpy99I CGWCG 1 cut(s) 110
HpyCH4IV ACGT 4 cut(s) 477, 820, 2310, 2524
HpySE526I ACGT 4 cut(s) 477, 820, 2310, 2524
HspAI GCGC 7 cut(s) 339, 495, 551, 2224, 2741, 2985, 3097
KpnI GGTACC 1 cut(s) 670
Ksp22I TGATCA 1 cut(s) 1567
KspI CCGCGG 1 cut(s) 2892
LguI GCTCTTC 2 cut(s) 807, 3297
LmnI GCTCC 5 cut(s) 1745, 1939, 2275, 3133, 3222
Lsp1109I GCAGC 6 cut(s) 194, 1343, 1386, 1816, 2238, 3095
LweI GCATC 8 cut(s) 268, 531, 538, 1092, 1857, 2863, 2996, 3283
MaeI CTAG 5 cut(s) 1028, 1094, 1227, 2199, 2834
MaeII ACGT 4 cut(s) 477, 820, 2310, 2524
MaeIII GTNAC 7 cut(s) 54, 355, 1359, 1834, 2829, 3064, 3319
MfeI CAATTG 2 cut(s) 1392, 3180
MflI RGATCY 5 cut(s) 988, 1906, 2044, 2379, 3333
MhlI GDGCHC 3 cut(s) 1456, 2590, 3365
MlyI GAGTC 3 cut(s) 874, 1106, 2683
MmeI TCCRAC 1 cut(s) 88
MroXI GAANNNNTTC 4 cut(s) 628, 1474, 1497, 2304
MslI CAYNNNNRTG 2 cut(s) 77, 2595
MspA1I CMGCKG 5 cut(s) 887, 1377, 2549, 2891, 3341
MspI CCGG 5 cut(s) 14, 45, 710, 1659, 2362
MspR9I CCNGG 7 cut(s) 15, 46, 710, 1120, 1533, 1842, 3070
MunI CAATTG 2 cut(s) 1392, 3180
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 4 cut(s) 1120, 1533, 1842, 3070
MvnI CGCG 3 cut(s) 1209, 2166, 2891
NciI CCSGG 3 cut(s) 15, 46, 710
NcoI CCATGG 3 cut(s) 1481, 2292, 3103
NdeI CATATG 1 cut(s) 1594
NlaIV GGNNCC 7 cut(s) 668, 1647, 1908, 2046, 2290, 3129, 3364
NmuCI GTSAC 4 cut(s) 54, 355, 3064, 3319
NruI TCGCGA 1 cut(s) 2166
NsbI TGCGCA 3 cut(s) 552, 2225, 2986
NspV TTCGAA 1 cut(s) 622
PagI TCATGA 2 cut(s) 295, 808
PceI AGGCCT 1 cut(s) 1972
PciSI GCTCTTC 2 cut(s) 807, 3297
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 4 cut(s) 628, 1474, 1497, 2304
PflMI CCANNNNNTGG 1 cut(s) 91
PfoI TCCNGGA 3 cut(s) 13, 708, 1531
PkrI GCNGC 7 cut(s) 184, 1333, 1376, 1806, 2228, 2890, 3110
PleI GAGTC 3 cut(s) 874, 1105, 2683
PpsI GAGTC 3 cut(s) 874, 1105, 2683
Psp6I CCWGG 4 cut(s) 1118, 1531, 1840, 3068
PspFI CCCAGC 1 cut(s) 3337
PspGI CCWGG 4 cut(s) 1118, 1531, 1840, 3068
PspN4I GGNNCC 7 cut(s) 668, 1647, 1908, 2046, 2290, 3129, 3364
PspPI GGNCC 5 cut(s) 82, 1090, 1646, 1888, 2540
PstI CTGCAG 1 cut(s) 862
PsuI RGATCY 5 cut(s) 988, 1906, 2044, 2379, 3333
PvuII CAGCTG 4 cut(s) 887, 1377, 2549, 3341
RruI TCGCGA 1 cut(s) 2166
RsaI GTAC 8 cut(s) 287, 512, 668, 1125, 1552, 1628, 2789, 2815
RsaNI GTAC 8 cut(s) 286, 511, 667, 1124, 1551, 1627, 2788, 2814
RseI CAYNNNNRTG 2 cut(s) 77, 2595
SacII CCGCGG 1 cut(s) 2892
SapI GCTCTTC 2 cut(s) 807, 3297
SatI GCNGC 7 cut(s) 183, 1332, 1375, 1805, 2227, 2889, 3109
Sau96I GGNCC 5 cut(s) 82, 1090, 1646, 1888, 2540
ScaI AGTACT 1 cut(s) 1628
SchI GAGTC 3 cut(s) 874, 1106, 2683
ScrFI CCNGG 7 cut(s) 15, 46, 710, 1120, 1533, 1842, 3070
SduI GDGCHC 3 cut(s) 1456, 2590, 3365
SfaNI GCATC 8 cut(s) 268, 531, 538, 1092, 1857, 2863, 2996, 3283
SfcI CTRYAG 6 cut(s) 696, 858, 913, 1158, 2091, 2793
Sfr303I CCGCGG 1 cut(s) 2892
SfuI TTCGAA 1 cut(s) 622
SgrBI CCGCGG 1 cut(s) 2892
SinI GGWCC 2 cut(s) 82, 1888
SmiMI CAYNNNNRTG 2 cut(s) 77, 2595
SmlI CTYRAG 2 cut(s) 1457, 2691
SmoI CTYRAG 2 cut(s) 1457, 2691
SseBI AGGCCT 1 cut(s) 1972
SsiI CCGC 8 cut(s) 440, 1017, 1712, 2124, 2412, 2889, 2891, 2965
SspI AATATT 5 cut(s) 1244, 2024, 2080, 2109, 2520
SspMI CTAG 5 cut(s) 1028, 1094, 1227, 2199, 2834
StuI AGGCCT 1 cut(s) 1972
StyD4I CCNGG 7 cut(s) 13, 44, 708, 1118, 1531, 1840, 3068
StyI CCWWGG 4 cut(s) 1481, 2292, 3103, 3366
TaiI ACGT 4 cut(s) 480, 823, 2313, 2527
TaqI TCGA 8 cut(s) 523, 622, 1100, 1165, 1640, 2489, 2499, 2848
TatI WGTACW 5 cut(s) 285, 510, 1550, 1626, 2787
TauI GCSGC 1 cut(s) 2891
TseFI GTSAC 4 cut(s) 54, 355, 3064, 3319
TseI GCWGC 6 cut(s) 182, 1331, 1374, 1804, 2226, 3108
Tsp45I GTSAC 4 cut(s) 54, 355, 3064, 3319
TspDTI ATGAA 9 cut(s) 823, 825, 1254, 1281, 1503, 2024, 2529, 2607, 3303
TspGWI ACGGA 2 cut(s) 1250, 1880
Van91I CCANNNNNTGG 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 82, 1888
XagI CCTNNNNNAGG 1 cut(s) 1530
XapI RAATTY 4 cut(s) 332, 443, 2461, 2481
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmnI GAANNNNTTC 4 cut(s) 628, 1474, 1497, 2304
XspI CTAG 5 cut(s) 1028, 1094, 1227, 2199, 2834
ZrmI AGTACT 1 cut(s) 1628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.