Rorug02G0027600

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001234
Physical Location & Seq
Forward (+)
2205235 .. 2208617
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0027600.1

Sequence Viewer

Length: 1287 bp
ATGCACCAAAACGGCGTCGTTGTTCTCGACAACGGCGGCGGCCTAATCAAAGCCGGGTACGGCGGCGAGCGCGACCCCGCCGCCATAATCCCCAACTGCGTCTACCGCCCTCTCTCCTCCAAGAAATGGCTCACCCCTTCCCCCACCGACCCCATCGACCTCACCTCCGCCGCCGTCCGCCGCCCCATCGACCGCGGCTACCTTATCAACCCGGACCTCCAGCGCGAGATCTGGGCCCACCTCTTCTCCCAAGTCCTCCGGGTCAACCCGGCCCAATCCTCCCTCCTCCTAACCGAGCCCCTCTTCACCCTCCCCTCCATCCAACGCGCCGCCGACGAGCTCGTCTTCGAGGACTTCAACTTCGCCGCCCTCTACGTGGCCCACGCGCCCTCCCTGGCCCACCTCTACGAGGCGAGCCGCCGGCCCGTTAAGGCCCAATGCAGCCTCGTCGTCGATTGTGGATTCTCGTTCACCCACGCCGTGCCGGTGTTCCAGAACTTTCCGATCAACTACGCCGCGAAGAGAATTGACCTGGGGGGAAAGGCGTTGACCAATTACCTCAAGGAGCTCGTTTCGTACCGCTCTGTGAATGTTATGGACGAAACGTTTCTTATTGACGATGTGAAGGAGAAGCTCTGCTTTGTCTCCATGAATGTGACTCGTGACCTCCAGATTGCAAAGAAGCATGGAAAGGACAATGCTTTCAGGTGCACATATGTGTTGCCTGATGGTGTTACGCATACACAAGGGTATGTTAAAGACCCAGAAGAGGCAAAGAGGTACTTGGCCATAGGAGACAGTGATTCGCTTCAAGAAGTGGCCAAGAAGATGGATATGAATCGGGTTGAAGTTGTGGACAAGTTGGAGGAGCGAAAGAAGATTGATTTGAGCAAGAATGAATTCGACTTGACAAATGAAAGGTTCCTTGTGCCGGAGATGATCTTCCATCCTGCTGATTTGGGAATGAATCAGGCTGGACTAGCGGAATGCATTGTTCGAGCTGTCAGTTCATGCCATCCTCATCTTCACCCTGTACTTTATGAAAGCATCATTTTAACTGGCGGAAGCACATTGTTTCCTCGACTTGCTGATAGACTAGAGAGAGAGCTGCGGCCTCTTGTTCCTGATGACTATCAAGTGAAGATAACTCCCCAAGAAGACCCCATACTAGGTGTTTGGCGAGGGGGATCAGTTTTGGCATCAAGTCCTGATTTTGAAGCAATGTGTGTTACCAAGGCTGAGTATGAGGAGCTTGGATCTGCTCGATGTCGGAAGAGATTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

428

Amino Acids

47.99

Weight (kDa)

6.04

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 3 - 428 5.3e-95 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 582
AccI GTMKAC 1 cut(s) 102
AccII CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
AclI AACGTT 1 cut(s) 605
AclWI GGATC 2 cut(s) 1195, 1264
AcoI YGGCCR 2 cut(s) 786, 819
AcsI RAATTY 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 15
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 4 cut(s) 59, 578, 782, 1035
AfiI CCNNNNNNNGG 6 cut(s) 126, 376, 409, 769, 931, 1169
AgsI TTSAA 4 cut(s) 358, 812, 848, 1217
AjnI CCWGG 2 cut(s) 393, 531
AleI CACNNNNGTG 1 cut(s) 716
AluBI AGCT 6 cut(s) 340, 568, 634, 1001, 1108, 1252
AluI AGCT 6 cut(s) 340, 568, 634, 1001, 1108, 1252
Alw21I GWGCWC 3 cut(s) 342, 570, 713
Alw26I GTCTC 2 cut(s) 649, 789
Alw44I GTGCAC 1 cut(s) 709
AlwI GGATC 2 cut(s) 1195, 1264
ApaI GGGCCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 441, 1108
ApoI RAATTY 1 cut(s) 899
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 3 cut(s) 606, 899, 1277
AspLEI GCGC 4 cut(s) 72, 225, 329, 388
AspS9I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
AsuC2I CCSGG 4 cut(s) 55, 212, 260, 269
AsuHPI GGTGA 5 cut(s) 124, 154, 298, 463, 1019
AvaII GGWCC 1 cut(s) 214
BaeGI GKGCMC 2 cut(s) 238, 713
BalI TGGCCA 2 cut(s) 788, 821
BanII GRGCYC 4 cut(s) 238, 300, 342, 570
BarI GAAGNNNNNNTAC 2 cut(s) 1149, 1181
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 337, 1164
Bbv12I GWGCWC 3 cut(s) 342, 570, 713
BbvI GCAGC 2 cut(s) 453, 1095
BccI CCATC 7 cut(s) 161, 194, 326, 722, 823, 954, 1023
BceAI ACGGC 5 cut(s) 28, 49, 76, 158, 464
BcgI CGANNNNNNTGC 2 cut(s) 430, 464
BciT130I CCWGG 2 cut(s) 395, 533
BcnI CCSGG 4 cut(s) 55, 212, 260, 269
BcoDI GTCTC 2 cut(s) 649, 789
BfaI CTAG 3 cut(s) 980, 1097, 1169
BglII AGATCT 1 cut(s) 228
Bme1390I CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
BmiI GGNNCC 2 cut(s) 236, 923
BmrFI CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
BmsI GCATC 2 cut(s) 1056, 1208
BpiI GAAGAC 2 cut(s) 337, 1164
BpmI CTGGAG 2 cut(s) 203, 653
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 4 cut(s) 55, 212, 260, 269
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 2 cut(s) 837, 1131
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 4 cut(s) 193, 393, 532, 1233
BsaXI ACNNNNNCTCC 6 cut(s) 149, 179, 230, 260, 374, 404
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 376, 409, 769, 931, 1169
Bse118I RCCGGY 2 cut(s) 420, 484
Bse1I ACTGG 1 cut(s) 1063
Bse3DI GCAATG 1 cut(s) 1227
Bse8I GATNNNNATC 2 cut(s) 837, 1131
BseBI CCWGG 2 cut(s) 395, 533
BseDI CCNNGG 4 cut(s) 193, 393, 532, 1233
BseGI GGATG 3 cut(s) 318, 946, 1015
BseJI GATNNNNATC 2 cut(s) 837, 1131
BseLI CCNNNNNNNGG 6 cut(s) 126, 376, 409, 769, 931, 1169
BseMI GCAATG 1 cut(s) 1227
BseMII CTCAG 1 cut(s) 1230
BseNI ACTGG 1 cut(s) 1063
BseRI GAGGAG 4 cut(s) 106, 275, 881, 1262
BseSI GKGCMC 2 cut(s) 238, 713
BseXI GCAGC 2 cut(s) 453, 1095
Bsh1236I CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
Bsh1285I CGRYCG 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 3 cut(s) 342, 570, 713
BsiSI CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
BslI CCNNNNNNNGG 6 cut(s) 126, 376, 409, 769, 931, 1169
BsmAI GTCTC 2 cut(s) 649, 789
BsmI GAATGC 1 cut(s) 992
Bsp120I GGGCCC 1 cut(s) 234
Bsp1286I GDGCHC 5 cut(s) 238, 300, 342, 570, 713
Bsp143I GATC 5 cut(s) 228, 504, 939, 1187, 1256
BspCNI CTCAG 1 cut(s) 1231
BspFNI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
BspLI GGNNCC 2 cut(s) 236, 923
BspPI GGATC 2 cut(s) 1195, 1264
BsrBI CCGCTC 1 cut(s) 582
BsrDI GCAATG 1 cut(s) 1227
BsrFI RCCGGY 2 cut(s) 420, 484
BsrI ACTGG 1 cut(s) 1063
BssAI RCCGGY 2 cut(s) 420, 484
BssECI CCNNGG 4 cut(s) 193, 393, 532, 1233
BssMI GATC 5 cut(s) 228, 504, 939, 1187, 1256
BssNI GRCGYC 1 cut(s) 15
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 1 cut(s) 1233
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 395, 533
Bst4CI ACNGT 1 cut(s) 800
Bst6I CTCTTC 5 cut(s) 248, 308, 515, 762, 1268
BstACI GRCGYC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 68, 415, 422
BstDEI CTNAG 1 cut(s) 1239
BstDSI CCRYGG 1 cut(s) 193
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 3 cut(s) 318, 946, 1015
BstFNI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
BstHHI GCGC 4 cut(s) 72, 225, 329, 388
BstKTI GATC 5 cut(s) 231, 507, 942, 1190, 1259
BstMAI GTCTC 2 cut(s) 649, 789
BstMBI GATC 5 cut(s) 228, 504, 939, 1187, 1256
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 69, 105, 980
BstNI CCWGG 2 cut(s) 395, 533
BstSCI CCNGG 6 cut(s) 53, 210, 258, 267, 393, 531
BstSLI GKGCMC 2 cut(s) 238, 713
BstUI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
BstV1I GCAGC 2 cut(s) 453, 1095
BstV2I GAAGAC 2 cut(s) 337, 1164
BstX2I RGATCY 2 cut(s) 228, 1256
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 2 cut(s) 228, 1256
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 3 cut(s) 318, 946, 1015
BtsIMutI CAGTG 1 cut(s) 805
Cac8I GCNNGC 3 cut(s) 68, 415, 422
CfoI GCGC 4 cut(s) 72, 225, 329, 388
Cfr10I RCCGGY 2 cut(s) 420, 484
Cfr13I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
Cfr42I CCGCGG 1 cut(s) 196
CseI GACGC 2 cut(s) 4, 88
Csp6I GTAC 4 cut(s) 58, 577, 781, 1034
CviAII CATG 3 cut(s) 649, 686, 1011
CviQI GTAC 4 cut(s) 58, 577, 781, 1034
DdeI CTNAG 1 cut(s) 1239
DpnI GATC 5 cut(s) 230, 506, 941, 1189, 1258
DpnII GATC 5 cut(s) 228, 504, 939, 1187, 1256
DraIII CACNNNGTG 1 cut(s) 481
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 2 cut(s) 786, 819
Eam1104I CTCTTC 5 cut(s) 248, 308, 515, 762, 1268
EarI CTCTTC 5 cut(s) 248, 308, 515, 762, 1268
EciI GGCGGA 3 cut(s) 157, 167, 1077
Ecl136II GAGCTC 2 cut(s) 340, 568
Eco130I CCWWGG 1 cut(s) 1233
Eco24I GRGCYC 4 cut(s) 238, 300, 342, 570
Eco47I GGWCC 1 cut(s) 214
Eco53kI GAGCTC 2 cut(s) 340, 568
EcoICRI GAGCTC 2 cut(s) 340, 568
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 2 cut(s) 393, 531
EcoT14I CCWWGG 1 cut(s) 1233
EcoT22I ATGCAT 1 cut(s) 992
EcoT38I GRGCYC 4 cut(s) 238, 300, 342, 570
ErhI CCWWGG 1 cut(s) 1233
FaeI CATG 3 cut(s) 652, 689, 1014
FalI AAGNNNNNCTT 5 cut(s) 623, 655, 767, 799, 1265
FatI CATG 3 cut(s) 648, 685, 1010
FauI CCCGC 1 cut(s) 85
FauNDI CATATG 1 cut(s) 715
FblI GTMKAC 1 cut(s) 102
FokI GGATG 3 cut(s) 305, 933, 1002
FriOI GRGCYC 4 cut(s) 238, 300, 342, 570
FspBI CTAG 3 cut(s) 980, 1097, 1169
GlaI GCGC 4 cut(s) 71, 224, 328, 387
GsuI CTGGAG 2 cut(s) 203, 653
HapII CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
HgaI GACGC 2 cut(s) 4, 88
HhaI GCGC 4 cut(s) 72, 225, 329, 388
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 3 cut(s) 652, 689, 1014
Hin6I GCGC 4 cut(s) 70, 223, 327, 386
HinP1I GCGC 4 cut(s) 70, 223, 327, 386
HincII GTYRAC 2 cut(s) 265, 549
HindII GTYRAC 2 cut(s) 265, 549
HinfI GANTC 6 cut(s) 462, 658, 803, 838, 967, 1278
HpaII CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
HphI GGTGA 5 cut(s) 124, 154, 298, 463, 1019
Hpy166II GTNNAC 6 cut(s) 103, 265, 471, 549, 711, 856
Hpy188I TCNGA 2 cut(s) 504, 1272
Hpy188III TCNNGA 7 cut(s) 26, 493, 662, 670, 812, 1124, 1208
Hpy8I GTNNAC 6 cut(s) 103, 265, 471, 549, 711, 856
Hpy99I CGWCG 4 cut(s) 20, 338, 452, 455
HpyAV CCTTC 2 cut(s) 147, 619
HpyCH4III ACNGT 1 cut(s) 800
HpyCH4IV ACGT 2 cut(s) 375, 605
HpyCH4V TGCA 5 cut(s) 4, 441, 677, 711, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 105, 980
HpyF3I CTNAG 1 cut(s) 1239
HpySE526I ACGT 2 cut(s) 375, 605
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 3 cut(s) 652, 689, 1014
HspAI GCGC 4 cut(s) 70, 223, 327, 386
KroI GCCGGC 1 cut(s) 420
KroNI GCCGGC 1 cut(s) 422
KspI CCGCGG 1 cut(s) 196
Kzo9I GATC 5 cut(s) 228, 504, 939, 1187, 1256
LmnI GCTCC 3 cut(s) 565, 868, 1249
Lsp1109I GCAGC 2 cut(s) 453, 1095
LweI GCATC 2 cut(s) 1056, 1208
MaeI CTAG 3 cut(s) 980, 1097, 1169
MaeII ACGT 2 cut(s) 375, 605
MaeIII GTNAC 4 cut(s) 655, 662, 733, 1228
MalI GATC 5 cut(s) 230, 506, 941, 1189, 1258
MbiI CCGCTC 1 cut(s) 582
MboI GATC 5 cut(s) 228, 504, 939, 1187, 1256
MflI RGATCY 2 cut(s) 228, 1256
MhlI GDGCHC 5 cut(s) 238, 300, 342, 570, 713
MlsI TGGCCA 2 cut(s) 788, 821
MluCI AATT 3 cut(s) 525, 553, 899
MluNI TGGCCA 2 cut(s) 788, 821
MlyI GAGTC 1 cut(s) 652
MmeI TCCRAC 3 cut(s) 346, 843, 1250
Mox20I TGGCCA 2 cut(s) 788, 821
Mph1103I ATGCAT 1 cut(s) 992
MroNI GCCGGC 1 cut(s) 420
MroXI GAANNNNTTC 3 cut(s) 606, 899, 1277
MscI TGGCCA 2 cut(s) 788, 821
MseI TTAA 4 cut(s) 429, 756, 1055, 1285
MslI CAYNNNNRTG 2 cut(s) 653, 716
Msp20I TGGCCA 2 cut(s) 788, 821
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 7 cut(s) 54, 212, 259, 269, 421, 485, 932
MspR9I CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
Mva1269I GAATGC 1 cut(s) 992
MvaI CCWGG 2 cut(s) 395, 533
MvnI CGCG 6 cut(s) 72, 195, 225, 327, 386, 518
MwoI GCNNNNNNNGC 3 cut(s) 69, 105, 980
NaeI GCCGGC 1 cut(s) 422
NciI CCSGG 4 cut(s) 55, 212, 260, 269
NdeI CATATG 1 cut(s) 715
NdeII GATC 5 cut(s) 228, 504, 939, 1187, 1256
NgoMIV GCCGGC 1 cut(s) 420
NlaIII CATG 3 cut(s) 652, 689, 1014
NlaIV GGNNCC 2 cut(s) 236, 923
NmuCI GTSAC 2 cut(s) 655, 662
NsiI ATGCAT 1 cut(s) 992
OliI CACNNNNGTG 1 cut(s) 716
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 1 cut(s) 992
PdiI GCCGGC 1 cut(s) 422
PdmI GAANNNNTTC 3 cut(s) 606, 899, 1277
PfeI GAWTC 5 cut(s) 462, 803, 838, 967, 1278
PflMI CCANNNNNTGG 1 cut(s) 126
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 376
Psp124BI GAGCTC 2 cut(s) 342, 570
Psp1406I AACGTT 1 cut(s) 605
Psp6I CCWGG 2 cut(s) 393, 531
PspGI CCWGG 2 cut(s) 393, 531
PspN4I GGNNCC 2 cut(s) 236, 923
PspOMI GGGCCC 1 cut(s) 234
PspPI GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
PsuI RGATCY 2 cut(s) 228, 1256
RsaI GTAC 4 cut(s) 59, 578, 782, 1035
RsaNI GTAC 4 cut(s) 58, 577, 781, 1034
RseI CAYNNNNRTG 2 cut(s) 653, 716
SacI GAGCTC 2 cut(s) 342, 570
SacII CCGCGG 1 cut(s) 196
SaqAI TTAA 4 cut(s) 429, 756, 1055, 1285
Sau3AI GATC 5 cut(s) 228, 504, 939, 1187, 1256
Sau96I GGNCC 8 cut(s) 214, 234, 235, 271, 379, 397, 423, 433
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 6 cut(s) 55, 212, 260, 269, 395, 533
SduI GDGCHC 5 cut(s) 238, 300, 342, 570, 713
SfaNI GCATC 2 cut(s) 1056, 1208
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 653, 716
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 3 cut(s) 525, 553, 899
SspMI CTAG 3 cut(s) 980, 1097, 1169
SstI GAGCTC 2 cut(s) 342, 570
StyD4I CCNGG 6 cut(s) 53, 210, 258, 267, 393, 531
StyI CCWWGG 1 cut(s) 1233
TaaI ACNGT 1 cut(s) 800
TaiI ACGT 2 cut(s) 378, 608
TaqI TCGA 9 cut(s) 27, 156, 189, 348, 453, 903, 997, 1081, 1264
TasI AATT 3 cut(s) 525, 553, 899
TatI WGTACW 1 cut(s) 1033
TfiI GAWTC 5 cut(s) 462, 803, 838, 967, 1278
Tru1I TTAA 4 cut(s) 429, 756, 1055, 1285
Tru9I TTAA 4 cut(s) 429, 756, 1055, 1285
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 2 cut(s) 655, 662
TseI GCWGC 2 cut(s) 441, 1108
Tsp45I GTSAC 2 cut(s) 655, 662
TspDTI ATGAA 7 cut(s) 665, 851, 912, 930, 980, 999, 1056
TspRI CASTG 1 cut(s) 805
Van91I CCANNNNNTGG 1 cut(s) 126
VneI GTGCAC 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 214
XagI CCTNNNNNAGG 1 cut(s) 407
XapI RAATTY 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 3 cut(s) 606, 899, 1277
XspI CTAG 3 cut(s) 980, 1097, 1169
Zsp2I ATGCAT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.