Rorug01G0003400

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001234
Physical Location & Seq
Reverse (-)
695318 .. 696553
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0003400.1

Sequence Viewer

Length: 1236 bp
ATGATGGCTTCTATCCCTCTTAGTACTCGGGGCGTCGAGGACCGCGTGGTTTGGCACTATGACAAGAAAGGGCTATATCATGTTCGAAGTGGTTACCATGTGTATAACACTGCCATGGGACGTAAAGAGTGTGCATCAACTTCATCTGGGGACGGTGGTGGTGTAAGTTGTAAATACTGGAGCAAGGTGTGGGGTGCATGCATTCCTCCTAAAGCTAAGTCTTTTGTATGGAGACTCTTGAAGAATATCCTTCCTACAAAAGCTGCCCTTTTACAACGTAAAGTTGCTTTGGTTAATCATGAGTGCATGTTTTGCATGGCAGTCACGGAGACAGGTACACATTTATTTAAAAATTGTCATGATTTGCATTGCTTTTGGTTGTTGGGTCCCCTTAGATTACATGCAAAATCACACCCAGCCAAGAGTGTTCATGAGTGGGTGTTGGACATGATTGATTCTCTGGAGGTGGACCAACGTGACTTCTTCTTTATGGCATTATGGACCATCTGGACTGAGCGTAACAACTTGGTCTGGAAAGGAGCCAATTTCCAGCCAATGAATATGATCCACTGGGCTACCAAAAGGTTAGAAGAATTTCAGAAGTATCATCCAAAGCCAGTTAGGAAGCAGAAGAGACCGTTGTCTAAGTGGAAAAATCCTCCTAGTGGCCGCCTTAAAATCAATGTAGATGGAGCCTTCAGATCGGATGTTGGGCAAGGAGGCATTGGAGTAGTGGTCAGAAATGATGCTGGTATGGGTATTGCTGCTTTGGCTAGACCTTTTCTACATGCACACTCGGTCCTCAATATGGAAGCGGAGGCATGTAGGGCTGGTCTACTTCTTGGAATCCACCAAGGTTGGGCTGACATAGATATTGAAAGCGACTCTGCCATTCTGATTGCTGCGCTCAATAGCGGAGGGATGAATCTCTCTGAGGTAAGTCGAGTTTTAGATGATTGTAAGGAGTATCTGACTGCTTTTCAATCAGTTAGAATTCGGCATATTTACCGTGAAGCAAATGGTGTTGCAGATAGACTTGCTCACCTTGCTAGTGTTGGTTTTATGGATGATGTTTGGTTAGATGAGACTCCTGCTATTATTCAGGACGTTCTCTACGAGGACTATTGTGTTTGTTTTCCTGTAGCACGGGGTTCAGGTTTTATGTCCCCCCCGTTGCAAAACTCTACTATTAATATAATAAATGGAACCGGGCGTGGGGCTGAGCCTCCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L31e

Protein Analysis

411

Amino Acids

46.03

Weight (kDa)

8.78

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 57 - 126 2.8e-13 zinc-binding in reverse transcriptase
RVT_3 PF13456 228 - 349 1.5e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 835
AccII CGCG 1 cut(s) 45
AciI CCGC 4 cut(s) 43, 670, 815, 915
AclWI GGATC 1 cut(s) 559
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 2 cut(s) 593, 993
AcuI CTGAAG 1 cut(s) 682
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 2 cut(s) 25, 337
AfiI CCNNNNNNNGG 4 cut(s) 665, 808, 859, 1215
AgsI TTSAA 3 cut(s) 241, 878, 983
AluBI AGCT 3 cut(s) 215, 263, 1233
AluI AGCT 3 cut(s) 215, 263, 1233
Alw26I GTCTC 4 cut(s) 226, 323, 628, 1079
AlwI GGATC 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 1 cut(s) 667
ApeKI GCWGC 3 cut(s) 263, 764, 902
ApoI RAATTY 2 cut(s) 593, 993
AseI ATTAAT 1 cut(s) 1191
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 5 cut(s) 40, 386, 469, 501, 799
AsuC2I CCSGG 1 cut(s) 1210
AsuHPI GGTGA 1 cut(s) 1034
AsuII TTCGAA 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 5 cut(s) 40, 386, 469, 501, 799
BbvI GCAGC 3 cut(s) 250, 751, 889
BccI CCATC 2 cut(s) 512, 683
BcnI CCSGG 1 cut(s) 1210
BcoDI GTCTC 4 cut(s) 226, 323, 628, 1079
BfaI CTAG 4 cut(s) 663, 774, 1050, 1234
BfmI CTRYAG 1 cut(s) 1140
BisI GCNGC 4 cut(s) 264, 670, 765, 903
BlpI GCTNAGC 1 cut(s) 1221
BlsI GCNGC 4 cut(s) 265, 671, 766, 904
BmcAI AGTACT 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 1210
Bme18I GGWCC 5 cut(s) 40, 386, 469, 501, 799
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 5 cut(s) 40, 386, 469, 501, 799
BmiI GGNNCC 5 cut(s) 387, 388, 541, 694, 1207
BmrFI CCNGG 1 cut(s) 1210
BmrI ACTGGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 143, 736
BmuI ACTGGG 1 cut(s) 580
BoxI GACNNNNGTC 1 cut(s) 640
BpmI CTGGAG 2 cut(s) 199, 482
Bpu1102I GCTNAGC 1 cut(s) 1221
Bpu14I TTCGAA 1 cut(s) 85
BpuMI CCSGG 1 cut(s) 1210
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 2 cut(s) 114, 853
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 4 cut(s) 665, 808, 859, 1215
Bse1I ACTGG 3 cut(s) 182, 575, 617
Bse3DI GCAATG 1 cut(s) 367
BseDI CCNNGG 2 cut(s) 114, 853
BseGI GGATG 4 cut(s) 607, 712, 927, 1072
BseLI CCNNNNNNNGG 4 cut(s) 665, 808, 859, 1215
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 3 cut(s) 504, 924, 1212
BseNI ACTGG 3 cut(s) 182, 575, 617
BseXI GCAGC 3 cut(s) 250, 751, 889
BseYI CCCAGC 2 cut(s) 415, 1229
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 1209
BslFI GGGAC 4 cut(s) 132, 164, 372, 1150
BslI CCNNNNNNNGG 4 cut(s) 665, 808, 859, 1215
BsmAI GTCTC 4 cut(s) 226, 323, 628, 1079
BsmFI GGGAC 4 cut(s) 132, 164, 372, 1150
BsmI GAATGC 1 cut(s) 201
BsnI GGCC 1 cut(s) 669
Bso31I GGTCTC 1 cut(s) 628
BsoBI CYCGRG 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 85
Bsp143I GATC 2 cut(s) 564, 701
Bsp1720I GCTNAGC 1 cut(s) 1221
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 4 cut(s) 43, 670, 815, 915
BspANI GGCC 1 cut(s) 669
BspCNI CTCAG 3 cut(s) 505, 925, 1213
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 3 cut(s) 298, 358, 430
BspLI GGNNCC 5 cut(s) 387, 388, 541, 694, 1207
BspPI GGATC 1 cut(s) 559
BspT104I TTCGAA 1 cut(s) 85
BspTNI GGTCTC 1 cut(s) 628
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 3 cut(s) 182, 575, 617
BssECI CCNNGG 2 cut(s) 114, 853
BssMI GATC 2 cut(s) 564, 701
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 114, 853
Bst4CI ACNGT 3 cut(s) 155, 639, 1010
Bst6I CTCTTC 1 cut(s) 626
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 312
BstBI TTCGAA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 7 cut(s) 20, 216, 392, 513, 645, 933, 1221
BstDSI CCRYGG 1 cut(s) 114
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 4 cut(s) 607, 712, 927, 1072
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 2 cut(s) 567, 704
BstMAI GTCTC 4 cut(s) 226, 323, 628, 1079
BstMBI GATC 2 cut(s) 564, 701
BstMWI GCNNNNNNNGC 4 cut(s) 312, 770, 827, 1046
BstNSI RCATGY 5 cut(s) 201, 310, 404, 791, 825
BstPAI GACNNNNGTC 1 cut(s) 640
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 1208
BstSFI CTRYAG 1 cut(s) 1140
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 250, 751, 889
BsuRI GGCC 1 cut(s) 669
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 4 cut(s) 607, 712, 927, 1072
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 108, 568
Cac8I GCNNGC 1 cut(s) 199
CciI TCATGA 3 cut(s) 298, 358, 430
CfoI GCGC 1 cut(s) 907
Cfr13I GGNCC 5 cut(s) 40, 386, 469, 501, 799
CseI GACGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 24, 336
CspCI CAANNNNNGTGG 2 cut(s) 839, 874
CviQI GTAC 2 cut(s) 24, 336
DdeI CTNAG 7 cut(s) 20, 216, 392, 513, 645, 933, 1221
DpnI GATC 2 cut(s) 566, 703
DpnII GATC 2 cut(s) 564, 701
DraI TTTAAA 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
Eco130I CCWWGG 2 cut(s) 114, 853
Eco31I GGTCTC 1 cut(s) 628
Eco47I GGWCC 5 cut(s) 40, 386, 469, 501, 799
Eco57I CTGAAG 1 cut(s) 682
Eco88I CYCGRG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 993
EcoT14I CCWWGG 2 cut(s) 114, 853
EcoT22I ATGCAT 1 cut(s) 203
ErhI CCWWGG 2 cut(s) 114, 853
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 4 cut(s) 132, 164, 372, 1150
FblI GTMKAC 1 cut(s) 835
Fnu4HI GCNGC 4 cut(s) 264, 670, 765, 903
FokI GGATG 4 cut(s) 594, 719, 934, 1079
Fsp4HI GCNGC 4 cut(s) 264, 670, 765, 903
FspBI CTAG 4 cut(s) 663, 774, 1050, 1234
GlaI GCGC 1 cut(s) 906
GluI GCNGC 4 cut(s) 264, 670, 765, 903
GsaI CCCAGC 2 cut(s) 419, 1233
GsuI CTGGAG 2 cut(s) 199, 482
HaeIII GGCC 1 cut(s) 669
HapII CCGG 1 cut(s) 1209
HgaI GACGC 1 cut(s) 22
HhaI GCGC 1 cut(s) 907
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HinfI GANTC 6 cut(s) 234, 455, 846, 884, 925, 1087
HpaII CCGG 1 cut(s) 1209
HphI GGTGA 1 cut(s) 1034
Hpy166II GTNNAC 3 cut(s) 338, 469, 836
Hpy188I TCNGA 7 cut(s) 600, 701, 706, 740, 897, 934, 972
Hpy188III TCNNGA 8 cut(s) 238, 299, 359, 431, 461, 508, 532, 1103
Hpy8I GTNNAC 3 cut(s) 338, 469, 836
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 260, 706
HpyCH4III ACNGT 3 cut(s) 155, 639, 1010
HpyCH4IV ACGT 4 cut(s) 121, 277, 475, 1107
HpyF10VI GCNNNNNNNGC 4 cut(s) 312, 770, 827, 1046
HpyF3I CTNAG 7 cut(s) 20, 216, 392, 513, 645, 933, 1221
HpySE526I ACGT 4 cut(s) 121, 277, 475, 1107
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 1 cut(s) 905
KflI GGGWCCC 1 cut(s) 386
Kzo9I GATC 2 cut(s) 564, 701
LmnI GCTCC 3 cut(s) 180, 539, 692
Lsp1109I GCAGC 3 cut(s) 250, 751, 889
LweI GCATC 2 cut(s) 143, 736
MaeI CTAG 4 cut(s) 663, 774, 1050, 1234
MaeII ACGT 4 cut(s) 121, 277, 475, 1107
MaeIII GTNAC 4 cut(s) 92, 322, 476, 518
MalI GATC 2 cut(s) 566, 703
MboI GATC 2 cut(s) 564, 701
MboII GAAGA 4 cut(s) 253, 475, 602, 643
MluCI AATT 4 cut(s) 352, 544, 593, 993
MlyI GAGTC 3 cut(s) 228, 878, 1081
MmeI TCCRAC 1 cut(s) 423
Mph1103I ATGCAT 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 4 cut(s) 294, 348, 675, 1191
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 1 cut(s) 1209
MspR9I CCNGG 1 cut(s) 1210
Mva1269I GAATGC 1 cut(s) 201
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 312, 770, 827, 1046
NciI CCSGG 1 cut(s) 1210
NcoI CCATGG 1 cut(s) 114
NdeII GATC 2 cut(s) 564, 701
NlaIV GGNNCC 5 cut(s) 387, 388, 541, 694, 1207
NmuCI GTSAC 2 cut(s) 322, 476
NsiI ATGCAT 1 cut(s) 203
NspI RCATGY 5 cut(s) 201, 310, 404, 791, 825
NspV TTCGAA 1 cut(s) 85
PaeI GCATGC 1 cut(s) 201
PagI TCATGA 3 cut(s) 298, 358, 430
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 1113
PctI GAATGC 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 3 cut(s) 455, 846, 925
PkrI GCNGC 4 cut(s) 265, 671, 766, 904
PleI GAGTC 3 cut(s) 228, 878, 1081
PpsI GAGTC 3 cut(s) 228, 878, 1081
PpuMI RGGWCCY 1 cut(s) 386
PshAI GACNNNNGTC 1 cut(s) 640
PshBI ATTAAT 1 cut(s) 1191
Psp5II RGGWCCY 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 92
PspFI CCCAGC 2 cut(s) 415, 1229
PspN4I GGNNCC 5 cut(s) 387, 388, 541, 694, 1207
PspPI GGNCC 5 cut(s) 40, 386, 469, 501, 799
PspPPI RGGWCCY 1 cut(s) 386
RsaI GTAC 2 cut(s) 25, 337
RsaNI GTAC 2 cut(s) 24, 336
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 4 cut(s) 294, 348, 675, 1191
SatI GCNGC 4 cut(s) 264, 670, 765, 903
Sau3AI GATC 2 cut(s) 564, 701
Sau96I GGNCC 5 cut(s) 40, 386, 469, 501, 799
ScaI AGTACT 1 cut(s) 25
SchI GAGTC 3 cut(s) 228, 878, 1081
ScrFI CCNGG 1 cut(s) 1210
SfaNI GCATC 2 cut(s) 143, 736
SfcI CTRYAG 1 cut(s) 1140
SfuI TTCGAA 1 cut(s) 85
SinI GGWCC 5 cut(s) 40, 386, 469, 501, 799
SmiMI CAYNNNNRTG 1 cut(s) 113
SphI GCATGC 1 cut(s) 201
Sse9I AATT 4 cut(s) 352, 544, 593, 993
SsiI CCGC 4 cut(s) 43, 670, 815, 915
SspMI CTAG 4 cut(s) 663, 774, 1050, 1234
StyD4I CCNGG 1 cut(s) 1208
StyI CCWWGG 2 cut(s) 114, 853
TaaI ACNGT 3 cut(s) 155, 639, 1010
TaiI ACGT 4 cut(s) 124, 280, 478, 1110
TaqI TCGA 3 cut(s) 36, 85, 943
TaqII GACCGA 1 cut(s) 787
TasI AATT 4 cut(s) 352, 544, 593, 993
TatI WGTACW 1 cut(s) 23
TauI GCSGC 1 cut(s) 672
TfiI GAWTC 3 cut(s) 455, 846, 925
Tru1I TTAA 4 cut(s) 294, 348, 675, 1191
Tru9I TTAA 4 cut(s) 294, 348, 675, 1191
TscAI CASTG 2 cut(s) 115, 575
TseFI GTSAC 2 cut(s) 322, 476
TseI GCWGC 3 cut(s) 263, 764, 902
Tsp45I GTSAC 2 cut(s) 322, 476
TspDTI ATGAA 4 cut(s) 132, 419, 572, 938
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 2 cut(s) 115, 575
VpaK11BI GGWCC 5 cut(s) 40, 386, 469, 501, 799
VspI ATTAAT 1 cut(s) 1191
XapI RAATTY 2 cut(s) 593, 993
XceI RCATGY 5 cut(s) 201, 310, 404, 791, 825
XmiI GTMKAC 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 4 cut(s) 663, 774, 1050, 1234
ZrmI AGTACT 1 cut(s) 25
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.