Rorug05G0453700

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001234
Physical Location & Seq
Forward (+)
62724370 .. 62725362
993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0453700.1

Sequence Viewer

Length: 465 bp
ATGTCGCTCATCCCAAATTTCCGACGAAACAGCATCTACGACCTCGATCTCTGGGATCCATTCAGGGATTTCCAATTTCCTTCCTCATCGCTCTCCACATTCCCTGAATTTCCTGGCGAAAATTCGGCTTTCATCAACACCCGGATCGACTGGAAGGAGACCCCAGAAGCCCATGTGTTCAAGGCCGACCTTCCGGGGCTGAAGAAAGAAGAGGTCAAGGTTGAGATTGAAGATGACAGGGTGCTGCAGATCAGCGGAGAGAGGAAGATAGAGAAGGAAGACAAGAACGACAAGTGGCACCGGGTCGAGAGAAGCAGCGGCAAGTTCTCCAGGAGATTCAGGCTTCCTGAGAATGCGAAGGTTGATGAGGTTAAGGCTGCTATGGAGAATGGGGTTCTCAGTGTGACTGTTCCTAAGGCAGAGGTGAAGAGGCCTGATGTCAAAGCCATTGAAATTTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L31e

Protein Analysis

154

Amino Acids

17.82

Weight (kDa)

5.83

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 50 - 153 3.7e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 297
AciI CCGC 2 cut(s) 255, 318
AclWI GGATC 3 cut(s) 50, 63, 152
AcsI RAATTY 4 cut(s) 16, 107, 121, 453
AcuI CTGAAG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 181, 230, 452
AjnI CCWGG 2 cut(s) 112, 329
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 3 cut(s) 50, 63, 152
AoxI GGCC 2 cut(s) 183, 431
ApeKI GCWGC 3 cut(s) 244, 315, 377
ApoI RAATTY 4 cut(s) 16, 107, 121, 453
AsuC2I CCSGG 3 cut(s) 142, 195, 302
AsuHPI GGTGA 1 cut(s) 436
AxyI CCTNAGG 1 cut(s) 414
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 285
BbvI GCAGC 3 cut(s) 231, 327, 364
BciT130I CCWGG 2 cut(s) 114, 331
BcnI CCSGG 3 cut(s) 142, 195, 302
BcoDI GTCTC 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 245
BisI GCNGC 4 cut(s) 245, 316, 319, 378
BlsI GCNGC 4 cut(s) 246, 317, 320, 379
Bme1390I CCNGG 5 cut(s) 114, 142, 195, 302, 331
BmiI GGNNCC 2 cut(s) 57, 299
BmrFI CCNGG 5 cut(s) 114, 142, 195, 302, 331
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 313
BpuMI CCSGG 3 cut(s) 142, 195, 302
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 155
Bse21I CCTNAGG 1 cut(s) 414
BseBI CCWGG 2 cut(s) 114, 331
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 339, 412
BseNI ACTGG 1 cut(s) 155
BseXI GCAGC 3 cut(s) 231, 327, 364
BshFI GGCC 2 cut(s) 185, 433
BshNI GGYRCC 1 cut(s) 297
BsiSI CCGG 3 cut(s) 142, 194, 301
BsmAI GTCTC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 2 cut(s) 185, 433
Bso31I GGTCTC 1 cut(s) 152
Bsp143I GATC 4 cut(s) 46, 55, 144, 249
BspACI CCGC 2 cut(s) 255, 318
BspANI GGCC 2 cut(s) 185, 433
BspCNI CTCAG 2 cut(s) 340, 411
BspLI GGNNCC 2 cut(s) 57, 299
BspMAI CTGCAG 1 cut(s) 249
BspPI GGATC 3 cut(s) 50, 63, 152
BspT107I GGYRCC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 152
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 4 cut(s) 46, 55, 144, 249
Bst2UI CCWGG 2 cut(s) 114, 331
Bst4CI ACNGT 1 cut(s) 409
Bst6I CTCTTC 2 cut(s) 204, 422
BstDEI CTNAG 3 cut(s) 348, 398, 414
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 4 cut(s) 49, 58, 147, 252
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 4 cut(s) 46, 55, 144, 249
BstNI CCWGG 2 cut(s) 114, 331
BstSCI CCNGG 5 cut(s) 112, 140, 193, 300, 329
BstSFI CTRYAG 1 cut(s) 245
BstV1I GCAGC 3 cut(s) 231, 327, 364
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 185, 433
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 406
CviAII CATG 1 cut(s) 173
CviJI RGCY 8 cut(s) 128, 170, 185, 199, 343, 377, 433, 446
CviKI_1 RGCY 8 cut(s) 128, 170, 185, 199, 343, 377, 433, 446
DdeI CTNAG 3 cut(s) 348, 398, 414
DpnI GATC 4 cut(s) 48, 57, 146, 251
DpnII GATC 4 cut(s) 46, 55, 144, 249
Eam1104I CTCTTC 2 cut(s) 204, 422
EarI CTCTTC 2 cut(s) 204, 422
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 221
Eco81I CCTNAGG 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 112, 329
FaeI CATG 1 cut(s) 176
FaiI YATR 2 cut(s) 174, 383
FatI CATG 1 cut(s) 172
Fnu4HI GCNGC 4 cut(s) 245, 316, 319, 378
Fsp4HI GCNGC 4 cut(s) 245, 316, 319, 378
GluI GCNGC 4 cut(s) 245, 316, 319, 378
GsuI CTGGAG 1 cut(s) 313
HaeIII GGCC 2 cut(s) 185, 433
HapII CCGG 3 cut(s) 142, 194, 301
Hin1II CATG 1 cut(s) 176
HinfI GANTC 1 cut(s) 336
HpaII CCGG 3 cut(s) 142, 194, 301
HphI GGTGA 1 cut(s) 436
Hpy188I TCNGA 1 cut(s) 23
Hpy188III TCNNGA 2 cut(s) 307, 347
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 5 cut(s) 90, 148, 200, 268, 352
HpyCH4III ACNGT 1 cut(s) 409
HpyCH4V TGCA 1 cut(s) 247
HpyF3I CTNAG 3 cut(s) 348, 398, 414
Hsp92II CATG 1 cut(s) 176
Kzo9I GATC 4 cut(s) 46, 55, 144, 249
Lsp1109I GCAGC 3 cut(s) 231, 327, 364
LweI GCATC 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 403
MalI GATC 4 cut(s) 48, 57, 146, 251
MboI GATC 4 cut(s) 46, 55, 144, 249
MboII GAAGA 6 cut(s) 214, 221, 242, 277, 290, 439
MflI RGATCY 1 cut(s) 55
MluCI AATT 5 cut(s) 16, 74, 107, 121, 453
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 7 cut(s) 53, 94, 205, 255, 361, 415, 423
MseI TTAA 2 cut(s) 372, 463
MspA1I CMGCKG 2 cut(s) 255, 318
MspI CCGG 3 cut(s) 142, 194, 301
MspR9I CCNGG 5 cut(s) 114, 142, 195, 302, 331
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 2 cut(s) 114, 331
NciI CCSGG 3 cut(s) 142, 195, 302
NdeII GATC 4 cut(s) 46, 55, 144, 249
NlaIII CATG 1 cut(s) 176
NlaIV GGNNCC 2 cut(s) 57, 299
NmuCI GTSAC 1 cut(s) 403
PceI AGGCCT 1 cut(s) 433
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 336
PfoI TCCNGGA 1 cut(s) 329
PkrI GCNGC 4 cut(s) 246, 317, 320, 379
Psp6I CCWGG 2 cut(s) 112, 329
PspGI CCWGG 2 cut(s) 112, 329
PspN4I GGNNCC 2 cut(s) 57, 299
PstI CTGCAG 1 cut(s) 249
PsuI RGATCY 1 cut(s) 55
SaqAI TTAA 2 cut(s) 372, 463
SatI GCNGC 4 cut(s) 245, 316, 319, 378
Sau3AI GATC 4 cut(s) 46, 55, 144, 249
ScrFI CCNGG 5 cut(s) 114, 142, 195, 302, 331
SetI ASST 7 cut(s) 45, 192, 216, 222, 363, 372, 426
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 1 cut(s) 245
Sse9I AATT 5 cut(s) 16, 74, 107, 121, 453
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 2 cut(s) 255, 318
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 5 cut(s) 112, 140, 193, 300, 329
TaaI ACNGT 1 cut(s) 409
TaqI TCGA 3 cut(s) 45, 147, 306
TasI AATT 5 cut(s) 16, 74, 107, 121, 453
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 2 cut(s) 372, 463
Tru9I TTAA 2 cut(s) 372, 463
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 3 cut(s) 244, 315, 377
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 1 cut(s) 406
XapI RAATTY 4 cut(s) 16, 107, 121, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.