pycom02g11620

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008672
Physical Location & Seq
Forward (+)
8202390 .. 8209799
7410 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3039 bp
ATGTCTTATTCACATTCTGTGTTCTGTCCTGTCCTCCCCGTTGTCTTCTTCTCTCCCGCCACCTCTTCCACTAAAACCCTAAACCCTAAATTACCAGAGGCTTTACCAGTCACCAAAACTACCCCGTTTCAGCTCCCAGAGCAGAACCAAAGCCAGCGAAGAACAATGGAGCCCGCTACGCTGACGAACTCAATGGACCTAAAGATTGGGGAGCTCTTGAAAGAGGTCCAGCTCGACTATTCACCCGCATTCACCAAGGCCGTCGACGACGCCGTTTCATTCATCAAAAGAGCCATCGACAAAATCCCAGAAGGCCTCAATGTTACAGCTGATGAAGCGCGGGGATTCGTCAGGGACATTGGCGCTGACAAGGTCGAATTCGCGTTTAAGAAGCCGAAATCTATTGAAATTGGTGGGAGTTATGCCATACAGTGCGTTGTAAAACCTGAAGTTAATGTGGACCTCTTCGTTCGGATGCCCAAGGAGTGTTTCCATGAGAAAGACTATTTGAATTACCGGTACCATGCGAAAAGGTGCCTGTACCTTTGTGTAATAAAAAAGTTTTTGAAGTCTTCTTCATTAGTTGAATGGTCCACCTTACAGAATGAAGCCCGGAAACCTGTATTGATTGTCTATCCAGGCGTAAAACTTGTCGAAGTCCCTGAGTTTTGTGTAAGGATAATTCCTACAGCACCATCCCTTTTCAACATTCCAAAGTTGCACCTAGAGCGCAATAATGTTCGTGCTTTAAATCAAGGGGGAGTTCCTCAGGCTACTCCTAAGTACAATAGTAGTATTTTGGAAGACATGCTCATTGAAGATACTGAAGAAATTCTCAAGAAAACTTTTCTCGGGTGGAAAGAATTACAAGAGGCATTAATTTTGCTGAAGGTCTGGGCTCGTCGGAGGACACCAATATATGCTTATGATTGCTTGGGTGGATATCTGATCTCTGTCATTTTGTCATACCTTGTGGATAGGAATCGTATTAAAAAGTCAATGACGACAATGCACATATTCCGTTTTAGCCTGAGCTTCCTTGCCAATTCAGAACTTTGGAAACATGGGCTCTACTTTACACCGAAAGACCAAAAAGCTATTCCAAAGGAGAAAAGGTTGCCTCTTAAAGAATCATTTCCAGTTGTTATATGTAGTCCAGCTACAAACTTTAATTTGGCCTTCCGAATGACAAGGGCTGGATTTCTCGAGCTCCAAGATGAGTCTGCTTCGACACTTACATGCATAGACAAATGTAGAGATGGTGGATTTGAGGAAATTTTTATGACCACGGTGGACTATCCTGTAAAATATGACCACATCATAAGGTTGAACTTAAAGGGGAACAGTGCAGTTTATGCATCAGGATTCTGCTTGGACGATGAATGTTGGAGATTATATGAGCAGAAGGTGCAGAGTGTTTTAATTCAAGGGTTGAGTGACAGAGTGAAGAATGTACGTGTTACCTGGAGAAATATGTTATCAGACCGCATCATAAAAAATGGATTGTCAACCCTCAATGCGGAGCCATTGCTTATTGGAATTTCCGTAAGCTCCATAGAGAAAGCTTTTAGGCTTGTTGATATTGGTCCAGATGCTGATAACAAAGCGGAGGCCCTCAAATTCCGCAAGTTCTGGGGGGAAAAGTCTGAGCTCAGGAGATTTAAAGATGGTAAAATTGCAGAAAGCACAGATCCTGTATCATCTTCTGGGAATTTGCTTGGGGCATTTGAAATTCTATCGAAGCGCTTGCGGCTTCTTGAAGACATTCCACTGAAGGTTTCTACTGTGCAGCCCCTAGATTCAGCTTTCAGGTTTTCTTCTGTATTCCCACCTGAACCTCATCCGCTTGCTAATGAGAAGGGTGCTTTTGTAAGATTACATAGGTTCACTCCTTCCTGCATTCGTCCGCTGGAAGTTATGATCCAGTTGGAAGGTTCTGGAAACTGGCCAATGGATGATCTAGCAATTGAGAAAACTAAATCTGCCTTCCTTCTCAAGATTGGAGAGAGTCTCCAGAATAATTGGGGGATGACGTGTACAGCTACGGAGGATGATGTGGATGTTTTTGTTTCTGGATATGCATTTCGCCTAAAAATTTGGCATGAGAGAGGCCTGACTTTGTTGAGACGGGAAACTGGAAATGATCTAGTGAAGCAGGTCTCTAATACGGACAGAGAACTTTATTTCCGGAGTCAACATTCCAGCATGATTAATGGATTACAGGGTTGTTATGCAGCATACGGGCCAGTTGTTAGGCTTGCAAAACGGTGGGTAGCTTCACATCTGTTTTCGGCTTGCTTGGGGGAGGAGGCAATTGAACTATTGGTTGCATACGTCTTTTTGAAGCCTTTACCATTCAATGCTCCATGCTCACGAATCACTGGATTTTTGAGGTTCCTACGATTACTATCAGATTATGACTGGAATTTTTCTGCATTAATTGTTGACATAAACAACGATTTGACTCCAAAAGATATGCAAGAGATAAATGATAATTTCATGTCAAGTAGAAAAACTAACGAAGAAAATGTGCTAAGTGTAATCCCAGCAATGTTCTTGGCTACAACTTATGACAAGGCATCTGAGGCCTGGACCAGATTCTCACCAAACTTGATGGAGTTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAAGTTGTTGACCAAACTGATATCGGAGGATCATGATGATAAGCATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTATGACGCAGTTATTCTTCTTCACAGAGAAAAATTACCTTACCCCCAACATCTTCTCTTTCCATCCGAATTAAATCAAGGTGCAGGGGTACATGTGGCATGTGGGAATGCGAGCAACGTTTTCCACCCATTTCTGTTGCCTGGAGACATGAAAGGAAATTCGGAGGCACTAAGAAATAAGCTATTGGTAAACTTTGATCCACTGAGGTGCTATGTCGGGGATCTAGAGGCAAGTTTTATAAAGAGTACCCCAACACCTTCAAGCTATGGTATGATTCTCTGGGAGGTGATGCTGTTGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1013

Amino Acids

114.85

Weight (kDa)

8.52

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 153 - 282 5.2e-35 Nrap protein domain 1
Nrap_D2 PF17403 289 - 429 1.4e-27 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 435 - 563 1e-27 Nrap protein domain 3
Nrap_D4 PF17405 560 - 741 5.5e-46 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 744 - 907 7.3e-47 Nrap protein PAP/OAS1-like domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2975
Acc36I ACCTGC 1 cut(s) 2146
Acc65I GGTACC 1 cut(s) 519
AccB1I GGYRCC 2 cut(s) 519, 534
AccI GTMKAC 1 cut(s) 264
AccII CGCG 2 cut(s) 340, 383
AccIII TCCGGA 1 cut(s) 2187
AclI AACGTT 1 cut(s) 2853
AclWI GGATC 5 cut(s) 1685, 1915, 2691, 2927, 2964
AcoI YGGCCR 1 cut(s) 1946
AcuI CTGAAG 4 cut(s) 468, 846, 908, 1793
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 7 cut(s) 521, 542, 785, 1455, 2038, 2826, 2983
AfeI AGCGCT 1 cut(s) 1745
AfiI CCNNNNNNNGG 1 cut(s) 1519
AflIII ACRYGT 3 cut(s) 1456, 2033, 2827
AgeI ACCGGT 1 cut(s) 516
AjiI CACGTC 1 cut(s) 2034
AjnI CCWGG 4 cut(s) 637, 1463, 2588, 2875
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 1083, 1115
AleI CACNNNNGTG 1 cut(s) 2941
AloI GAACNNNNNNTCC 2 cut(s) 2169, 2201
Alw21I GWGCWC 3 cut(s) 216, 1212, 1653
Alw26I GTCTC 4 cut(s) 2015, 2119, 2164, 2874
AlwI GGATC 5 cut(s) 1685, 1915, 2691, 2927, 2964
AlwNI CAGNNNCTG 2 cut(s) 1595, 1694
Ama87I CYCGRG 2 cut(s) 851, 1205
Aor13HI TCCGGA 1 cut(s) 2187
Aor51HI AGCGCT 1 cut(s) 1745
AoxI GGCC 8 cut(s) 258, 313, 1176, 1611, 1946, 2110, 2243, 2586
ApeKI GCWGC 2 cut(s) 1789, 2234
AseI ATTAAT 3 cut(s) 878, 2211, 2438
AsiGI ACCGGT 1 cut(s) 516
Asp700I GAANNNNTTC 3 cut(s) 831, 1134, 1764
Asp718I GGTACC 1 cut(s) 519
AspLEI GCGC 4 cut(s) 340, 365, 732, 1746
AspS9I GGNCC 8 cut(s) 196, 226, 460, 591, 1586, 1612, 2243, 2592
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 5 cut(s) 103, 234, 244, 2595, 3034
AsuII TTCGAA 1 cut(s) 2719
AvaI CYCGRG 2 cut(s) 851, 1205
AvaII GGWCC 6 cut(s) 196, 226, 460, 591, 1586, 2592
AxyI CCTNAGG 1 cut(s) 768
BalI TGGCCA 1 cut(s) 1948
BanI GGYRCC 2 cut(s) 519, 534
BanII GRGCYC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
BbsI GAAGAC 4 cut(s) 37, 564, 810, 1767
Bbv12I GWGCWC 3 cut(s) 216, 1212, 1653
BbvI GCAGC 2 cut(s) 1801, 2246
BccI CCATC 8 cut(s) 302, 703, 1253, 1661, 2608, 2626, 2697, 2806
BceAI ACGGC 2 cut(s) 245, 257
BcgI CGANNNNNNTGC 2 cut(s) 1729, 1763
BciT130I CCWGG 4 cut(s) 639, 1465, 2590, 2877
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 4 cut(s) 2015, 2119, 2164, 2874
BfaI CTAG 5 cut(s) 725, 1796, 1961, 2147, 2960
BfmI CTRYAG 1 cut(s) 687
BfoI RGCGCY 2 cut(s) 366, 1747
BfuAI ACCTGC 1 cut(s) 2146
BisI GCNGC 3 cut(s) 1751, 1790, 2235
BlsI GCNGC 3 cut(s) 1752, 1791, 2236
Bme1390I CCNGG 5 cut(s) 613, 639, 1465, 2590, 2877
Bme18I GGWCC 6 cut(s) 196, 226, 460, 591, 1586, 2592
BmeT110I CYCGRG 2 cut(s) 851, 1205
BmgBI CACGTC 1 cut(s) 2034
BmgT120I GGNCC 8 cut(s) 196, 226, 460, 591, 1586, 1612, 2243, 2592
BmiI GGNNCC 5 cut(s) 171, 521, 536, 1524, 2396
BmrFI CCNGG 5 cut(s) 613, 639, 1465, 2590, 2877
BmsI GCATC 6 cut(s) 465, 1367, 1497, 1582, 2588, 3015
BpiI GAAGAC 4 cut(s) 37, 564, 810, 1767
BplI GAGNNNNNCTC 2 cut(s) 1995, 2027
BpmI CTGGAG 3 cut(s) 1486, 1997, 2898
Bpu10I CCTNAGC 2 cut(s) 1031, 1652
Bpu14I TTCGAA 1 cut(s) 2719
BpuEI CTTGAG 2 cut(s) 821, 1979
BpuMI CCSGG 1 cut(s) 613
BsaAI YACGTR 1 cut(s) 1457
BsaBI GATNNNNATC 2 cut(s) 981, 2407
BsaHI GRCGYC 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 2164
BsaJI CCNNGG 3 cut(s) 255, 480, 1287
BsaWI WCCGGW 2 cut(s) 516, 2187
BsaXI ACNNNNNCTCC 2 cut(s) 1100, 1130
Bsc4I CCNNNNNNNGG 1 cut(s) 1519
Bse118I RCCGGY 1 cut(s) 516
Bse1I ACTGG 8 cut(s) 107, 1139, 1924, 1949, 2140, 2246, 2386, 2426
Bse21I CCTNAGG 1 cut(s) 768
Bse3DI GCAATG 2 cut(s) 1526, 2556
Bse8I GATNNNNATC 2 cut(s) 981, 2407
BseAI TCCGGA 1 cut(s) 2187
BseBI CCWGG 4 cut(s) 639, 1465, 2590, 2877
BseDI CCNNGG 3 cut(s) 255, 480, 1287
BseGI GGATG 8 cut(s) 480, 695, 1840, 1960, 2034, 2056, 2065, 2798
BseJI GATNNNNATC 2 cut(s) 981, 2407
BseLI CCNNNNNNNGG 1 cut(s) 1519
BseMI GCAATG 2 cut(s) 1526, 2556
BseMII CTCAG 7 cut(s) 654, 782, 1022, 1638, 1666, 2574, 2930
BseNI ACTGG 8 cut(s) 107, 1139, 1924, 1949, 2140, 2246, 2386, 2426
BseRI GAGGAG 1 cut(s) 2321
BseXI GCAGC 2 cut(s) 1801, 2246
BseYI CCCAGC 1 cut(s) 2545
BsgI GTGCAG 4 cut(s) 1368, 1430, 1808, 2838
Bsh1236I CGCG 2 cut(s) 340, 383
BshFI GGCC 8 cut(s) 260, 315, 1178, 1613, 1948, 2112, 2245, 2588
BshNI GGYRCC 2 cut(s) 519, 534
BshTI ACCGGT 1 cut(s) 516
BsiHKAI GWGCWC 3 cut(s) 216, 1212, 1653
BsiHKCI CYCGRG 2 cut(s) 851, 1205
BsiSI CCGG 3 cut(s) 517, 613, 2188
BslFI GGGAC 2 cut(s) 368, 644
BslI CCNNNNNNNGG 1 cut(s) 1519
BsmAI GTCTC 4 cut(s) 2015, 2119, 2164, 2874
BsmBI CGTCTC 1 cut(s) 2119
BsmFI GGGAC 2 cut(s) 368, 644
BsmI GAATGC 3 cut(s) 248, 1899, 2848
BsnI GGCC 8 cut(s) 260, 315, 1178, 1613, 1948, 2112, 2245, 2588
Bso31I GGTCTC 1 cut(s) 2164
BsoBI CYCGRG 2 cut(s) 851, 1205
Bsp119I TTCGAA 1 cut(s) 2719
Bsp1286I GDGCHC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
Bsp13I TCCGGA 1 cut(s) 2187
Bsp1407I TGTACA 1 cut(s) 2036
Bsp143I GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
BspANI GGCC 8 cut(s) 260, 315, 1178, 1613, 1948, 2112, 2245, 2588
BspCNI CTCAG 7 cut(s) 655, 781, 1023, 1639, 1665, 2575, 2931
BspEI TCCGGA 1 cut(s) 2187
BspFNI CGCG 2 cut(s) 340, 383
BspHI TCATGA 1 cut(s) 2686
BspLI GGNNCC 5 cut(s) 171, 521, 536, 1524, 2396
BspMI ACCTGC 1 cut(s) 2146
BspPI GGATC 5 cut(s) 1685, 1915, 2691, 2927, 2964
BspT104I TTCGAA 1 cut(s) 2719
BspT107I GGYRCC 2 cut(s) 519, 534
BspTNI GGTCTC 1 cut(s) 2164
BsrDI GCAATG 2 cut(s) 1526, 2556
BsrFI RCCGGY 1 cut(s) 516
BsrGI TGTACA 1 cut(s) 2036
BsrI ACTGG 8 cut(s) 107, 1139, 1924, 1949, 2140, 2246, 2386, 2426
BssAI RCCGGY 1 cut(s) 516
BssECI CCNNGG 3 cut(s) 255, 480, 1287
BssMI GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 2 cut(s) 255, 480
Bst2UI CCWGG 4 cut(s) 639, 1465, 2590, 2877
Bst4CI ACNGT 5 cut(s) 432, 1291, 1346, 1786, 2268
Bst6I CTCTTC 2 cut(s) 70, 470
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 2 cut(s) 1355, 1408
BstAUI TGTACA 1 cut(s) 2036
BstBAI YACGTR 1 cut(s) 1457
BstBI TTCGAA 1 cut(s) 2719
BstC8I GCNNGC 7 cut(s) 155, 174, 1748, 1848, 2259, 2296, 2848
BstDSI CCRYGG 1 cut(s) 1287
BstF5I GGATG 8 cut(s) 480, 695, 1840, 1960, 2034, 2056, 2065, 2798
BstFNI CGCG 2 cut(s) 340, 383
BstH2I RGCGCY 2 cut(s) 366, 1747
BstHHI GCGC 4 cut(s) 340, 365, 732, 1746
BstKTI GATC 8 cut(s) 951, 1693, 1923, 1960, 2146, 2686, 2935, 2959
BstMAI GTCTC 4 cut(s) 2015, 2119, 2164, 2874
BstMBI GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
BstMWI GCNNNNNNNGC 8 cut(s) 139, 178, 335, 727, 1355, 1408, 1750, 2585
BstNI CCWGG 4 cut(s) 639, 1465, 2590, 2877
BstNSI RCATGY 4 cut(s) 811, 1242, 2831, 2838
BstSCI CCNGG 5 cut(s) 611, 637, 1463, 2588, 2875
BstSFI CTRYAG 1 cut(s) 687
BstUI CGCG 2 cut(s) 340, 383
BstV1I GCAGC 2 cut(s) 1801, 2246
BstV2I GAAGAC 4 cut(s) 37, 564, 810, 1767
BstX2I RGATCY 2 cut(s) 1690, 2956
BstYI RGATCY 2 cut(s) 1690, 2956
Bsu36I CCTNAGG 1 cut(s) 768
BsuRI GGCC 8 cut(s) 260, 315, 1178, 1613, 1948, 2112, 2245, 2588
BtgI CCRYGG 1 cut(s) 1287
BtrI CACGTC 1 cut(s) 2034
BtsCI GGATG 8 cut(s) 480, 695, 1840, 1960, 2034, 2056, 2065, 2798
BtsI GCAGTG 1 cut(s) 2656
BtsIMutI CAGTG 6 cut(s) 437, 1351, 1769, 2379, 2656, 2936
BveI ACCTGC 1 cut(s) 2146
Cac8I GCNNGC 7 cut(s) 155, 174, 1748, 1848, 2259, 2296, 2848
CaiI CAGNNNCTG 2 cut(s) 1595, 1694
CciI TCATGA 1 cut(s) 2686
CfoI GCGC 4 cut(s) 340, 365, 732, 1746
Cfr10I RCCGGY 1 cut(s) 516
Cfr13I GGNCC 8 cut(s) 196, 226, 460, 591, 1586, 1612, 2243, 2592
CseI GACGC 2 cut(s) 278, 2750
Csp6I GTAC 7 cut(s) 520, 541, 784, 1454, 2037, 2825, 2982
CspAI ACCGGT 1 cut(s) 516
CviQI GTAC 7 cut(s) 520, 541, 784, 1454, 2037, 2825, 2982
DpnI GATC 8 cut(s) 950, 1692, 1922, 1959, 2145, 2685, 2934, 2958
DpnII GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
DraI TTTAAA 2 cut(s) 750, 1663
EaeI YGGCCR 1 cut(s) 1946
Eam1104I CTCTTC 2 cut(s) 70, 470
EarI CTCTTC 2 cut(s) 70, 470
Ecl136II GAGCTC 3 cut(s) 214, 1210, 1651
Eco130I CCWWGG 2 cut(s) 255, 480
Eco147I AGGCCT 3 cut(s) 315, 2112, 2588
Eco24I GRGCYC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
Eco31I GGTCTC 1 cut(s) 2164
Eco32I GATATC 2 cut(s) 944, 2676
Eco47I GGWCC 6 cut(s) 196, 226, 460, 591, 1586, 2592
Eco47III AGCGCT 1 cut(s) 1745
Eco53kI GAGCTC 3 cut(s) 214, 1210, 1651
Eco57I CTGAAG 4 cut(s) 468, 846, 908, 1793
Eco81I CCTNAGG 1 cut(s) 768
Eco88I CYCGRG 2 cut(s) 851, 1205
EcoICRI GAGCTC 3 cut(s) 214, 1210, 1651
EcoO109I RGGNCCY 1 cut(s) 1612
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 4 cut(s) 637, 1463, 2588, 2875
EcoRV GATATC 2 cut(s) 944, 2676
EcoT14I CCWWGG 2 cut(s) 255, 480
EcoT22I ATGCAT 3 cut(s) 1244, 1360, 2083
EcoT38I GRGCYC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
ErhI CCWWGG 2 cut(s) 255, 480
Esp3I CGTCTC 1 cut(s) 2119
FaqI GGGAC 2 cut(s) 368, 644
FauI CCCGC 4 cut(s) 64, 181, 253, 333
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 3 cut(s) 1751, 1790, 2235
FokI GGATG 8 cut(s) 487, 682, 1827, 1967, 2041, 2063, 2072, 2785
FriOI GRGCYC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
Fsp4HI GCNGC 3 cut(s) 1751, 1790, 2235
FspBI CTAG 5 cut(s) 725, 1796, 1961, 2147, 2960
GlaI GCGC 4 cut(s) 339, 364, 731, 1745
GluI GCNGC 3 cut(s) 1751, 1790, 2235
GsaI CCCAGC 1 cut(s) 2549
GsuI CTGGAG 3 cut(s) 1486, 1997, 2898
HaeII RGCGCY 2 cut(s) 366, 1747
HaeIII GGCC 8 cut(s) 260, 315, 1178, 1613, 1948, 2112, 2245, 2588
HapII CCGG 3 cut(s) 517, 613, 2188
HgaI GACGC 2 cut(s) 278, 2750
HhaI GCGC 4 cut(s) 340, 365, 732, 1746
Hin1I GRCGYC 1 cut(s) 270
Hin6I GCGC 4 cut(s) 338, 363, 730, 1744
HinP1I GCGC 4 cut(s) 338, 363, 730, 1744
HincII GTYRAC 5 cut(s) 265, 1509, 2195, 2446, 2664
HindII GTYRAC 5 cut(s) 265, 1509, 2195, 2446, 2664
HindIII AAGCTT 1 cut(s) 1563
HpaII CCGG 3 cut(s) 517, 613, 2188
HphI GGTGA 5 cut(s) 103, 234, 244, 2595, 3034
Hpy99I CGWCG 4 cut(s) 266, 269, 272, 906
HpyCH4III ACNGT 5 cut(s) 432, 1291, 1346, 1786, 2268
HpyCH4IV ACGT 4 cut(s) 1456, 2033, 2334, 2853
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 178, 335, 727, 1355, 1408, 1750, 2585
HpySE526I ACGT 4 cut(s) 1456, 2033, 2334, 2853
Hsp92I GRCGYC 1 cut(s) 270
HspAI GCGC 4 cut(s) 338, 363, 730, 1744
Kpn2I TCCGGA 1 cut(s) 2187
KpnI GGTACC 1 cut(s) 523
Kzo9I GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
LmnI GCTCC 7 cut(s) 138, 169, 211, 1215, 1522, 1556, 2368
Lsp1109I GCAGC 2 cut(s) 1801, 2246
LweI GCATC 6 cut(s) 465, 1367, 1497, 1582, 2588, 3015
MaeI CTAG 5 cut(s) 725, 1796, 1961, 2147, 2960
MaeII ACGT 4 cut(s) 1456, 2033, 2334, 2853
MaeIII GTNAC 4 cut(s) 109, 322, 1436, 1459
MalI GATC 8 cut(s) 950, 1692, 1922, 1959, 2145, 2685, 2934, 2958
MboI GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
MfeI CAATTG 2 cut(s) 1965, 2313
MflI RGATCY 2 cut(s) 1690, 2956
MhlI GDGCHC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
MlsI TGGCCA 1 cut(s) 1948
MluNI TGGCCA 1 cut(s) 1948
MlyI GAGTC 4 cut(s) 1229, 2017, 2200, 2458
MmeI TCCRAC 3 cut(s) 884, 1367, 1908
Mox20I TGGCCA 1 cut(s) 1948
Mph1103I ATGCAT 3 cut(s) 1244, 1360, 2083
MroI TCCGGA 1 cut(s) 2187
MroXI GAANNNNTTC 3 cut(s) 831, 1134, 1764
MscI TGGCCA 1 cut(s) 1948
MslI CAYNNNNRTG 2 cut(s) 1237, 2941
Msp20I TGGCCA 1 cut(s) 1948
MspA1I CMGCKG 2 cut(s) 329, 1909
MspI CCGG 3 cut(s) 517, 613, 2188
MspR9I CCNGG 5 cut(s) 613, 639, 1465, 2590, 2877
MunI CAATTG 2 cut(s) 1965, 2313
Mva1269I GAATGC 3 cut(s) 248, 1899, 2848
MvaI CCWGG 4 cut(s) 639, 1465, 2590, 2877
MvnI CGCG 2 cut(s) 340, 383
MwoI GCNNNNNNNGC 8 cut(s) 139, 178, 335, 727, 1355, 1408, 1750, 2585
NciI CCSGG 1 cut(s) 613
NdeII GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
NlaIV GGNNCC 5 cut(s) 171, 521, 536, 1524, 2396
NmuCI GTSAC 2 cut(s) 109, 1436
NsiI ATGCAT 3 cut(s) 1244, 1360, 2083
NspI RCATGY 4 cut(s) 811, 1242, 2831, 2838
NspV TTCGAA 1 cut(s) 2719
OliI CACNNNNGTG 1 cut(s) 2941
PaeR7I CTCGAG 1 cut(s) 1205
PagI TCATGA 1 cut(s) 2686
PceI AGGCCT 3 cut(s) 315, 2112, 2588
PciI ACATGT 1 cut(s) 2827
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 3 cut(s) 248, 1899, 2848
PdmI GAANNNNTTC 3 cut(s) 831, 1134, 1764
PfeI GAWTC 8 cut(s) 345, 982, 1130, 1365, 1799, 2376, 2598, 3010
PflFI GACNNNGTC 1 cut(s) 371
PinAI ACCGGT 1 cut(s) 516
PkrI GCNGC 3 cut(s) 1752, 1791, 2236
PleI GAGTC 4 cut(s) 1228, 2016, 2199, 2458
PpsI GAGTC 4 cut(s) 1228, 2016, 2199, 2458
Ppu21I YACGTR 1 cut(s) 1457
PscI ACATGT 1 cut(s) 2827
PshBI ATTAAT 3 cut(s) 878, 2211, 2438
PsiI TTATAA 1 cut(s) 2975
Psp124BI GAGCTC 3 cut(s) 216, 1212, 1653
Psp1406I AACGTT 1 cut(s) 2853
Psp6I CCWGG 4 cut(s) 637, 1463, 2588, 2875
PspFI CCCAGC 1 cut(s) 2545
PspGI CCWGG 4 cut(s) 637, 1463, 2588, 2875
PspN4I GGNNCC 5 cut(s) 171, 521, 536, 1524, 2396
PspPI GGNCC 8 cut(s) 196, 226, 460, 591, 1586, 1612, 2243, 2592
PstNI CAGNNNCTG 2 cut(s) 1595, 1694
PsuI RGATCY 2 cut(s) 1690, 2956
PsyI GACNNNGTC 1 cut(s) 371
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 7 cut(s) 521, 542, 785, 1455, 2038, 2826, 2983
RsaNI GTAC 7 cut(s) 520, 541, 784, 1454, 2037, 2825, 2982
RseI CAYNNNNRTG 2 cut(s) 1237, 2941
SacI GAGCTC 3 cut(s) 216, 1212, 1653
SalI GTCGAC 1 cut(s) 263
SatI GCNGC 3 cut(s) 1751, 1790, 2235
Sau3AI GATC 8 cut(s) 948, 1690, 1920, 1957, 2143, 2683, 2932, 2956
Sau96I GGNCC 8 cut(s) 196, 226, 460, 591, 1586, 1612, 2243, 2592
SchI GAGTC 4 cut(s) 1229, 2017, 2200, 2458
ScrFI CCNGG 5 cut(s) 613, 639, 1465, 2590, 2877
SduI GDGCHC 6 cut(s) 174, 216, 901, 1071, 1212, 1653
SfaNI GCATC 6 cut(s) 465, 1367, 1497, 1582, 2588, 3015
SfcI CTRYAG 1 cut(s) 687
Sfr274I CTCGAG 1 cut(s) 1205
SfuI TTCGAA 1 cut(s) 2719
SgrDI CGTCGACG 1 cut(s) 263
SinI GGWCC 6 cut(s) 196, 226, 460, 591, 1586, 2592
SlaI CTCGAG 1 cut(s) 1205
SmiMI CAYNNNNRTG 2 cut(s) 1237, 2941
SmlI CTYRAG 3 cut(s) 836, 1205, 1994
SmoI CTYRAG 3 cut(s) 836, 1205, 1994
SseBI AGGCCT 3 cut(s) 315, 2112, 2588
SspMI CTAG 5 cut(s) 725, 1796, 1961, 2147, 2960
SstI GAGCTC 3 cut(s) 216, 1212, 1653
StuI AGGCCT 3 cut(s) 315, 2112, 2588
StyD4I CCNGG 5 cut(s) 611, 637, 1463, 2588, 2875
StyI CCWWGG 2 cut(s) 255, 480
TaaI ACNGT 5 cut(s) 432, 1291, 1346, 1786, 2268
TaiI ACGT 4 cut(s) 1459, 2036, 2337, 2856
TaqI TCGA 9 cut(s) 234, 264, 297, 375, 654, 1206, 1229, 1739, 2719
TatI WGTACW 2 cut(s) 783, 2036
TauI GCSGC 1 cut(s) 1753
TfiI GAWTC 8 cut(s) 345, 982, 1130, 1365, 1799, 2376, 2598, 3010
TscAI CASTG 6 cut(s) 437, 1351, 1776, 2386, 2656, 2943
TseFI GTSAC 2 cut(s) 109, 1436
TseI GCWGC 2 cut(s) 1789, 2234
Tsp45I GTSAC 2 cut(s) 109, 1436
TspDTI ATGAA 8 cut(s) 267, 271, 348, 567, 621, 1395, 2488, 2900
TspGWI ACGGA 4 cut(s) 1010, 1534, 2060, 2183
TspRI CASTG 6 cut(s) 437, 1351, 1776, 2386, 2656, 2943
Tth111I GACNNNGTC 1 cut(s) 371
VpaK11BI GGWCC 6 cut(s) 196, 226, 460, 591, 1586, 2592
VspI ATTAAT 3 cut(s) 878, 2211, 2438
XbaI TCTAGA 1 cut(s) 2959
XceI RCATGY 4 cut(s) 811, 1242, 2831, 2838
XhoI CTCGAG 1 cut(s) 1205
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 3 cut(s) 831, 1134, 1764
XspI CTAG 5 cut(s) 725, 1796, 1961, 2147, 2960
Zsp2I ATGCAT 3 cut(s) 1244, 1360, 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.