pycom02g26490
ERF Family

and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009235
Physical Location & Seq
Reverse (-)
24258015 .. 24260726
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26490.2

Sequence Viewer

Length: 1050 bp
ATGGAGCTGACGGTGGTAGATCTGGGCGCGTACTTGGAATCGGAGGAGGCCGGAGTGAAGGAGTTGTGCGGCGAGGTTAGTCGGAGTCTGAGAGAAACGGGAGCTCTGTTGGTAAAGGATCCCAGATGTACGGCGGAGGACAACGATCGCTTCCTTGATATGATGGAGAGGTACTTCGACAGTCCGCCGGAGTTCAAGCGACTCCAGGAGAGGCCCCAATTGCATTACCAGGTTGGTGTGACACCGGAAGGCGTGGAAGTCCCCAGAAGCCTTGTTGATGAAGAAATGCAGGAGAAATTGAAGGAAATACCCAAAGAGTCCCAGCCATCCATCCCCAAAGGGGCAGATCGCAAATGGCGATACATGTGGAGAGTGGGTCCTCGACCCTCGAAAACCCGCTTTCAGGAGCTTAATGCAGAGCCCGTCGTACCCGAGGAGTTTCCTGAATGGAAAGATACCATGGATTCGTGGGGTTACAAGATGATTTCCGCAATAGAGGCTGTTGCTGAAATGGCTGCCATTGGATTTGGCTTGCCAAAGGATGCATTCACTTCTCTTATGAAGCAGGGACCGCACCTTCTAGCTCCAACAGGAAGTGACCTTCGGCGTTATGGCCAAGAGGGTACTGTGTTTGCAGGATATCATTATGACCTCAACTTTATAACTATTCACGGCAGAAGTAGATTCCCAGGTCTGAATATTTGGCTTAAAAATGGGCAAAAAGTTGAGGTGAAGGTTCCTGTAGGATGTCTTCTCATTCAGACTGGGAAGCAGATAGAATGGCTGACTGCAGGAGATTGCATAGCTGGAATGCATGAAGTTATCGTAACAAACAGGACGATTGATGCAATCAAACTAGCAATGGAGCAAAATCGCTTACTATGGAGAGTATCTTCAACATTGTTTGCGCACATAGCATCTGATGCTGTGTTGAAGCCCTTAGGTCACTTTGCAGAATCCCCGCTTGCAAGTAAATACCCGCCCATTCATGCAGGAGAGTTTGTTGAACAGGAGCTTGCAGTAATCAATCTCAAAGGAAACAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIOX_N

Protein Analysis

350

Amino Acids

39.35

Weight (kDa)

5.54

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 662
Acc16I TGCGCA 1 cut(s) 909
AccII CGCG 1 cut(s) 29
AciI CCGC 8 cut(s) 69, 134, 185, 397, 489, 572, 962, 980
AclWI GGATC 2 cut(s) 113, 126
AcoI YGGCCR 1 cut(s) 613
AfaI GTAC 5 cut(s) 32, 130, 173, 429, 625
AfiI CCNNNNNNNGG 2 cut(s) 340, 403
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 5 cut(s) 196, 301, 897, 934, 1007
AjnI CCWGG 3 cut(s) 204, 228, 688
AluBI AGCT 6 cut(s) 7, 104, 409, 584, 806, 1015
AluI AGCT 6 cut(s) 7, 104, 409, 584, 806, 1015
Alw21I GWGCWC 1 cut(s) 106
AlwI GGATC 2 cut(s) 113, 126
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 3 cut(s) 48, 212, 613
ApeKI GCWGC 1 cut(s) 515
AspLEI GCGC 2 cut(s) 29, 910
AspS9I GGNCC 3 cut(s) 213, 377, 569
AsuHPI GGTGA 1 cut(s) 742
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 2 cut(s) 377, 569
AxyI CCTNAGG 1 cut(s) 940
BalI TGGCCA 1 cut(s) 615
BamHI GGATCC 1 cut(s) 118
BanII GRGCYC 2 cut(s) 106, 423
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 502
BccI CCATC 3 cut(s) 157, 334, 338
BceAI ACGGC 2 cut(s) 147, 688
BciT130I CCWGG 3 cut(s) 206, 230, 690
BfaI CTAG 2 cut(s) 581, 857
BfmI CTRYAG 2 cut(s) 741, 789
BglII AGATCT 1 cut(s) 19
BisI GCNGC 2 cut(s) 70, 516
BlsI GCNGC 2 cut(s) 71, 517
Bme1390I CCNGG 3 cut(s) 206, 230, 690
Bme18I GGWCC 2 cut(s) 377, 569
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 3 cut(s) 213, 377, 569
BmiI GGNNCC 5 cut(s) 120, 215, 378, 570, 738
BmrFI CCNGG 3 cut(s) 206, 230, 690
BmrI ACTGGG 1 cut(s) 774
BmsI GCATC 4 cut(s) 532, 835, 913, 926
BmuI ACTGGG 1 cut(s) 774
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 432, 459, 688
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 361, 391, 987, 1017
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 403
Bse1I ACTGG 1 cut(s) 769
Bse21I CCTNAGG 1 cut(s) 940
Bse3DI GCAATG 1 cut(s) 867
BseBI CCWGG 3 cut(s) 206, 230, 690
BseDI CCNNGG 3 cut(s) 432, 459, 688
BseGI GGATG 4 cut(s) 326, 330, 547, 752
BseLI CCNNNNNNNGG 2 cut(s) 340, 403
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 769
BseRI GAGGAG 2 cut(s) 59, 449
BseXI GCAGC 1 cut(s) 502
BseYI CCCAGC 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 3 cut(s) 50, 214, 615
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 3 cut(s) 51, 188, 245
BslFI GGGAC 3 cut(s) 245, 304, 582
BslI CCNNNNNNNGG 2 cut(s) 340, 403
BsmFI GGGAC 3 cut(s) 245, 304, 582
BsmI GAATGC 2 cut(s) 545, 816
BsnI GGCC 3 cut(s) 50, 214, 615
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 106, 423
Bsp143I GATC 4 cut(s) 19, 118, 145, 346
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 8 cut(s) 69, 134, 185, 397, 489, 572, 962, 980
BspANI GGCC 3 cut(s) 50, 214, 615
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 5 cut(s) 120, 215, 378, 570, 738
BspMAI CTGCAG 1 cut(s) 793
BspPI GGATC 2 cut(s) 113, 126
BsrDI GCAATG 1 cut(s) 867
BsrI ACTGG 1 cut(s) 769
BssECI CCNNGG 3 cut(s) 432, 459, 688
BssMI GATC 4 cut(s) 19, 118, 145, 346
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 206, 230, 690
Bst4CI ACNGT 3 cut(s) 13, 182, 628
BstAPI GCANNNNNTGC 1 cut(s) 923
BstC8I GCNNGC 3 cut(s) 533, 966, 1017
BstDEI CTNAG 2 cut(s) 89, 940
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 326, 330, 547, 752
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 2 cut(s) 29, 910
BstKTI GATC 4 cut(s) 22, 121, 148, 349
BstMBI GATC 4 cut(s) 19, 118, 145, 346
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 6 cut(s) 220, 497, 512, 571, 914, 923
BstNI CCWGG 3 cut(s) 206, 230, 690
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 3 cut(s) 204, 228, 688
BstSFI CTRYAG 2 cut(s) 741, 789
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 19, 118
BstYI RGATCY 2 cut(s) 19, 118
Bsu36I CCTNAGG 1 cut(s) 940
BsuRI GGCC 3 cut(s) 50, 214, 615
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 326, 330, 547, 752
Cac8I GCNNGC 3 cut(s) 533, 966, 1017
CfoI GCGC 2 cut(s) 29, 910
Cfr13I GGNCC 3 cut(s) 213, 377, 569
CsiI ACCWGGT 1 cut(s) 228
Csp6I GTAC 5 cut(s) 31, 129, 172, 428, 624
CviAII CATG 4 cut(s) 364, 460, 815, 989
CviQI GTAC 5 cut(s) 31, 129, 172, 428, 624
DdeI CTNAG 2 cut(s) 89, 940
DpnI GATC 4 cut(s) 21, 120, 147, 348
DpnII GATC 4 cut(s) 19, 118, 145, 346
EaeI YGGCCR 1 cut(s) 613
EciI GGCGGA 2 cut(s) 149, 174
Ecl136II GAGCTC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 2 cut(s) 106, 423
Eco32I GATATC 1 cut(s) 641
Eco47I GGWCC 2 cut(s) 377, 569
Eco53kI GAGCTC 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 940
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 104
EcoO109I RGGNCCY 2 cut(s) 213, 377
EcoRII CCWGG 3 cut(s) 204, 228, 688
EcoRV GATATC 1 cut(s) 641
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 2 cut(s) 547, 816
EcoT38I GRGCYC 2 cut(s) 106, 423
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 4 cut(s) 367, 463, 818, 992
FaqI GGGAC 3 cut(s) 245, 304, 582
FatI CATG 4 cut(s) 363, 459, 814, 988
FauI CCCGC 3 cut(s) 404, 969, 987
Fnu4HI GCNGC 2 cut(s) 70, 516
FokI GGATG 4 cut(s) 313, 317, 554, 759
FriOI GRGCYC 2 cut(s) 106, 423
Fsp4HI GCNGC 2 cut(s) 70, 516
FspBI CTAG 2 cut(s) 581, 857
FspI TGCGCA 1 cut(s) 909
GlaI GCGC 2 cut(s) 28, 909
GluI GCNGC 2 cut(s) 70, 516
GsaI CCCAGC 1 cut(s) 325
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 3 cut(s) 50, 214, 615
HapII CCGG 3 cut(s) 51, 188, 245
HhaI GCGC 2 cut(s) 29, 910
Hin1II CATG 4 cut(s) 367, 463, 818, 992
Hin6I GCGC 2 cut(s) 27, 908
HinP1I GCGC 2 cut(s) 27, 908
HinfI GANTC 7 cut(s) 38, 85, 201, 317, 464, 684, 956
HpaII CCGG 3 cut(s) 51, 188, 245
HphI GGTGA 1 cut(s) 742
Hpy188I TCNGA 6 cut(s) 43, 84, 90, 696, 762, 922
Hpy188III TCNNGA 2 cut(s) 404, 443
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 6 cut(s) 52, 242, 295, 587, 611, 727
HpyCH4III ACNGT 3 cut(s) 13, 182, 628
HpyF10VI GCNNNNNNNGC 6 cut(s) 220, 497, 512, 571, 914, 923
HpyF3I CTNAG 2 cut(s) 89, 940
Hsp92II CATG 4 cut(s) 367, 463, 818, 992
HspAI GCGC 2 cut(s) 27, 908
Kzo9I GATC 4 cut(s) 19, 118, 145, 346
LmnI GCTCC 6 cut(s) 4, 101, 406, 589, 865, 1012
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 4 cut(s) 532, 835, 913, 926
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 2 cut(s) 581, 857
MaeIII GTNAC 5 cut(s) 238, 473, 596, 826, 944
MalI GATC 4 cut(s) 21, 120, 147, 348
MboI GATC 4 cut(s) 19, 118, 145, 346
MboII GAAGA 3 cut(s) 293, 743, 885
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 2 cut(s) 19, 118
MhlI GDGCHC 2 cut(s) 106, 423
MlsI TGGCCA 1 cut(s) 615
MluCI AATT 2 cut(s) 218, 296
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 3 cut(s) 94, 195, 326
MmeI TCCRAC 2 cut(s) 62, 611
Mox20I TGGCCA 1 cut(s) 615
Mph1103I ATGCAT 2 cut(s) 547, 816
MscI TGGCCA 1 cut(s) 615
MseI TTAA 3 cut(s) 411, 708, 1048
Msp20I TGGCCA 1 cut(s) 615
MspI CCGG 3 cut(s) 51, 188, 245
MspR9I CCNGG 3 cut(s) 206, 230, 690
MunI CAATTG 1 cut(s) 218
Mva1269I GAATGC 2 cut(s) 545, 816
MvaI CCWGG 3 cut(s) 206, 230, 690
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 6 cut(s) 220, 497, 512, 571, 914, 923
NcoI CCATGG 1 cut(s) 459
NdeII GATC 4 cut(s) 19, 118, 145, 346
NlaIII CATG 4 cut(s) 367, 463, 818, 992
NlaIV GGNNCC 5 cut(s) 120, 215, 378, 570, 738
NmuCI GTSAC 3 cut(s) 238, 596, 944
NsbI TGCGCA 1 cut(s) 909
NsiI ATGCAT 2 cut(s) 547, 816
NspI RCATGY 1 cut(s) 367
PciI ACATGT 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 355
PctI GAATGC 2 cut(s) 545, 816
PfeI GAWTC 4 cut(s) 38, 464, 684, 956
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 2 cut(s) 71, 517
Ple19I CGATCG 1 cut(s) 148
PleI GAGTC 3 cut(s) 93, 195, 325
PpsI GAGTC 3 cut(s) 93, 195, 325
PpuMI RGGWCCY 1 cut(s) 377
PscI ACATGT 1 cut(s) 363
PsiI TTATAA 1 cut(s) 662
Psp124BI GAGCTC 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 377
Psp6I CCWGG 3 cut(s) 204, 228, 688
PspFI CCCAGC 1 cut(s) 321
PspGI CCWGG 3 cut(s) 204, 228, 688
PspN4I GGNNCC 5 cut(s) 120, 215, 378, 570, 738
PspPI GGNCC 3 cut(s) 213, 377, 569
PspPPI RGGWCCY 1 cut(s) 377
PstI CTGCAG 1 cut(s) 793
PsuI RGATCY 2 cut(s) 19, 118
PvuI CGATCG 1 cut(s) 148
RsaI GTAC 5 cut(s) 32, 130, 173, 429, 625
RsaNI GTAC 5 cut(s) 31, 129, 172, 428, 624
SacI GAGCTC 1 cut(s) 106
SaqAI TTAA 3 cut(s) 411, 708, 1048
SatI GCNGC 2 cut(s) 70, 516
Sau3AI GATC 4 cut(s) 19, 118, 145, 346
Sau96I GGNCC 3 cut(s) 213, 377, 569
SchI GAGTC 3 cut(s) 94, 195, 326
ScrFI CCNGG 3 cut(s) 206, 230, 690
SduI GDGCHC 2 cut(s) 106, 423
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 4 cut(s) 532, 835, 913, 926
SfcI CTRYAG 2 cut(s) 741, 789
SinI GGWCC 2 cut(s) 377, 569
Sse9I AATT 2 cut(s) 218, 296
SsiI CCGC 8 cut(s) 69, 134, 185, 397, 489, 572, 962, 980
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 581, 857
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 3 cut(s) 204, 228, 688
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 3 cut(s) 13, 182, 628
TaqI TCGA 3 cut(s) 177, 382, 389
TasI AATT 2 cut(s) 218, 296
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 4 cut(s) 38, 464, 684, 956
Tru1I TTAA 3 cut(s) 411, 708, 1048
Tru9I TTAA 3 cut(s) 411, 708, 1048
TseFI GTSAC 3 cut(s) 238, 596, 944
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 3 cut(s) 238, 596, 944
TspDTI ATGAA 4 cut(s) 294, 575, 831, 977
VpaK11BI GGWCC 2 cut(s) 377, 569
XceI RCATGY 1 cut(s) 367
XspI CTAG 2 cut(s) 581, 857
Zsp2I ATGCAT 2 cut(s) 547, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.