RchiOBHm_Chr1g0314801
ERF Family

and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009235
Physical Location & Seq
Forward (+)
2123322 .. 2126140
2819 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54535

Sequence Viewer

Length: 1053 bp
ATGGAGCTGGCGGTGATAGATCTGAGCCCCTACTTGGAATCGGGATCGGAATTGAAGGAGCTGTGCGGCGAGGTCAGTCGGAGTCTCAGAGAAACCGGAGCTCTCTTAGTCAAGGACCCCCGATGTACGGCCCAGGACAACGATCGCTTCCTCGATATGATGGAGAACTACTTCGCCTCTTCTTCCGACTTCAAGCGACTCCACGAGAGGCCCCAATTGCATTACCAGGTCGGTGTGACTCCCGAGGGCGTGGAAGTGCCCAGAAGCCTGGTTGATGAGGAAATGCAGGAGAAATTGAAGCAAATGCCGAAACAAGTGCAGCCTTCCACTCCCAAGGGCCCTGATTGCAAATGGCGATACATGTGGAGAGTCGGTCCCAGACCATCCAAAACCCGCTTTCAGGAACTTAATGCGGAGCCAGTTGTACCTGAGGGTTTTCCTGAGTGGAAAGAAACCATGGATTCATGGGGGTACAAGATGATTTCGGCAATTGAGGCTGTTGCTGAAATGGCTGCCATTGGATTTGGTTTGCCAAAGGATGCATTCACTTCCCTTATGCAGCGGGGACCACACCTTCTAGCTCCAACTGGAAGTGACCTTCAGCGTTATGGCCAAGAGGGTACTGTCTTTGCAGGATATCACTATGACCTGAACTTTTTAACTATTCATGGCAGAAGTAGATTCCCAGGGCTTAATATCTGGCTAAGAAATGGGCAAAAAGTTGAAGTGAAGGTTCCTGTTGGATGTCTGCTTATTCAGACTGGAAAGCAGATAGAATGGTTAACTGGTGGAGATTGCATAGCTGGAATGCATGAAGTTGTTGTAACAGACAGGACAACTGATGCAATCAAACTAGCAAGGGAGCAAAATCGCATCTTATGGAGAGTATCTTCTACTTTGTTTGGCCACATAGCATCTGATGCTGTCTTGAAGCCCTTAGGCCACTTTGCCGATTCGCCACTTGCGAGTAAATATCCGCCCATCTATGCAGGGGAGTTTGTTGAACAAGAGCTTTCCGTAATCAATCTCAAAGGAAACAAAGGACAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIOX_N

Protein Analysis

350

Amino Acids

39.32

Weight (kDa)

5.97

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 11, 66, 394, 413, 562, 977
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 2 cut(s) 610, 904
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 4 cut(s) 127, 426, 473, 622
AfiI CCNNNNNNNGG 3 cut(s) 34, 127, 400
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 6 cut(s) 55, 193, 298, 725, 931, 1004
AjnI CCWGG 4 cut(s) 132, 225, 267, 685
AluBI AGCT 6 cut(s) 7, 61, 101, 581, 803, 1012
AluI AGCT 6 cut(s) 7, 61, 101, 581, 803, 1012
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 6 cut(s) 129, 209, 337, 610, 904, 940
ApaI GGGCCC 1 cut(s) 341
ApeKI GCWGC 3 cut(s) 319, 512, 559
Asp700I GAANNNNTTC 1 cut(s) 170
AspS9I GGNCC 7 cut(s) 115, 130, 210, 337, 338, 374, 566
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 3 cut(s) 115, 374, 566
AxyI CCTNAGG 2 cut(s) 429, 937
BaeGI GKGCMC 2 cut(s) 261, 341
BalI TGGCCA 2 cut(s) 612, 906
BanII GRGCYC 3 cut(s) 29, 103, 341
BauI CACGAG 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 331, 499, 571
BccI CCATC 3 cut(s) 154, 391, 989
BceAI ACGGC 1 cut(s) 144
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 4 cut(s) 134, 227, 269, 687
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 2 cut(s) 578, 854
BglII AGATCT 1 cut(s) 19
BisI GCNGC 4 cut(s) 67, 320, 513, 560
BlsI GCNGC 4 cut(s) 68, 321, 514, 561
Bme1390I CCNGG 4 cut(s) 134, 227, 269, 687
Bme18I GGWCC 3 cut(s) 115, 374, 566
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 7 cut(s) 115, 130, 210, 337, 338, 374, 566
BmiI GGNNCC 7 cut(s) 117, 212, 339, 376, 417, 567, 735
BmrFI CCNGG 4 cut(s) 134, 227, 269, 687
BmsI GCATC 5 cut(s) 529, 832, 882, 910, 923
BsaJI CCNNGG 6 cut(s) 132, 243, 333, 456, 685, 686
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 127, 400
Bse1I ACTGG 4 cut(s) 419, 592, 766, 790
Bse21I CCTNAGG 2 cut(s) 429, 937
BseBI CCWGG 4 cut(s) 134, 227, 269, 687
BseDI CCNNGG 6 cut(s) 132, 243, 333, 456, 685, 686
BseGI GGATG 3 cut(s) 383, 544, 749
BseLI CCNNNNNNNGG 3 cut(s) 34, 127, 400
BseMII CTCAG 4 cut(s) 14, 100, 420, 432
BseNI ACTGG 4 cut(s) 419, 592, 766, 790
BseSI GKGCMC 2 cut(s) 261, 341
BseXI GCAGC 3 cut(s) 331, 499, 571
BsgI GTGCAG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 6 cut(s) 131, 211, 339, 612, 906, 942
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 360, 579
BslI CCNNNNNNNGG 3 cut(s) 34, 127, 400
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 360, 579
BsmI GAATGC 2 cut(s) 542, 813
BsnI GGCC 6 cut(s) 131, 211, 339, 612, 906, 942
BsoBI CYCGRG 1 cut(s) 242
Bsp120I GGGCCC 1 cut(s) 337
Bsp1286I GDGCHC 4 cut(s) 29, 103, 261, 341
Bsp143I GATC 3 cut(s) 19, 44, 142
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 6 cut(s) 11, 66, 394, 413, 562, 977
BspANI GGCC 6 cut(s) 131, 211, 339, 612, 906, 942
BspCNI CTCAG 4 cut(s) 15, 99, 421, 433
BspLI GGNNCC 7 cut(s) 117, 212, 339, 376, 417, 567, 735
BspPI GGATC 1 cut(s) 52
BsrI ACTGG 4 cut(s) 419, 592, 766, 790
BssECI CCNNGG 6 cut(s) 132, 243, 333, 456, 685, 686
BssMI GATC 3 cut(s) 19, 44, 142
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 2 cut(s) 333, 456
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 4 cut(s) 134, 227, 269, 687
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 1 cut(s) 184
BstAPI GCANNNNNTGC 1 cut(s) 920
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 7 cut(s) 23, 86, 106, 429, 441, 704, 937
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 3 cut(s) 383, 544, 749
BstKTI GATC 3 cut(s) 22, 47, 145
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 3 cut(s) 19, 44, 142
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 5 cut(s) 217, 345, 494, 509, 920
BstNI CCWGG 4 cut(s) 134, 227, 269, 687
BstNSI RCATGY 1 cut(s) 364
BstSCI CCNGG 4 cut(s) 132, 225, 267, 685
BstSLI GKGCMC 2 cut(s) 261, 341
BstV1I GCAGC 3 cut(s) 331, 499, 571
BstX2I RGATCY 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 19
Bsu36I CCTNAGG 2 cut(s) 429, 937
BsuRI GGCC 6 cut(s) 131, 211, 339, 612, 906, 942
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 3 cut(s) 383, 544, 749
Cac8I GCNNGC 1 cut(s) 9
Cfr13I GGNCC 7 cut(s) 115, 130, 210, 337, 338, 374, 566
CsiI ACCWGGT 1 cut(s) 225
Csp6I GTAC 4 cut(s) 126, 425, 472, 621
CviAII CATG 5 cut(s) 361, 457, 465, 668, 812
CviQI GTAC 4 cut(s) 126, 425, 472, 621
DdeI CTNAG 7 cut(s) 23, 86, 106, 429, 441, 704, 937
DpnI GATC 3 cut(s) 21, 46, 144
DpnII GATC 3 cut(s) 19, 44, 142
EaeI YGGCCR 2 cut(s) 610, 904
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
EciI GGCGGA 1 cut(s) 966
Ecl136II GAGCTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 333, 456
Eco24I GRGCYC 3 cut(s) 29, 103, 341
Eco32I GATATC 1 cut(s) 638
Eco47I GGWCC 3 cut(s) 115, 374, 566
Eco53kI GAGCTC 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 584
Eco81I CCTNAGG 2 cut(s) 429, 937
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 101
EcoO109I RGGNCCY 4 cut(s) 115, 210, 337, 338
EcoRII CCWGG 4 cut(s) 132, 225, 267, 685
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 2 cut(s) 333, 456
EcoT22I ATGCAT 2 cut(s) 544, 813
EcoT38I GRGCYC 3 cut(s) 29, 103, 341
ErhI CCWWGG 2 cut(s) 333, 456
FaeI CATG 5 cut(s) 364, 460, 468, 671, 815
FalI AAGNNNNNCTT 1 cut(s) 1032
FaqI GGGAC 2 cut(s) 360, 579
FatI CATG 5 cut(s) 360, 456, 464, 667, 811
FauI CCCGC 2 cut(s) 401, 555
Fnu4HI GCNGC 4 cut(s) 67, 320, 513, 560
FokI GGATG 3 cut(s) 370, 551, 756
FriOI GRGCYC 3 cut(s) 29, 103, 341
Fsp4HI GCNGC 4 cut(s) 67, 320, 513, 560
FspBI CTAG 2 cut(s) 578, 854
GluI GCNGC 4 cut(s) 67, 320, 513, 560
HaeIII GGCC 6 cut(s) 131, 211, 339, 612, 906, 942
HapII CCGG 1 cut(s) 96
Hin1II CATG 5 cut(s) 364, 460, 468, 671, 815
HincII GTYRAC 1 cut(s) 783
HindII GTYRAC 1 cut(s) 783
HinfI GANTC 8 cut(s) 38, 82, 198, 238, 369, 461, 681, 953
HpaI GTTAAC 1 cut(s) 783
HpaII CCGG 1 cut(s) 96
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 783
Hpy188I TCNGA 7 cut(s) 24, 49, 81, 89, 187, 759, 919
Hpy188III TCNNGA 5 cut(s) 42, 242, 401, 440, 928
Hpy8I GTNNAC 1 cut(s) 783
HpyAV CCTTC 5 cut(s) 49, 333, 584, 608, 724
HpyCH4III ACNGT 1 cut(s) 625
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 345, 494, 509, 920
HpyF3I CTNAG 7 cut(s) 23, 86, 106, 429, 441, 704, 937
Hsp92II CATG 5 cut(s) 364, 460, 468, 671, 815
KspAI GTTAAC 1 cut(s) 783
Kzo9I GATC 3 cut(s) 19, 44, 142
LmnI GCTCC 6 cut(s) 4, 58, 98, 415, 586, 862
Lsp1109I GCAGC 3 cut(s) 331, 499, 571
LweI GCATC 5 cut(s) 529, 832, 882, 910, 923
MabI ACCWGGT 1 cut(s) 225
MaeI CTAG 2 cut(s) 578, 854
MaeIII GTNAC 3 cut(s) 235, 593, 823
MalI GATC 3 cut(s) 21, 46, 144
MboI GATC 3 cut(s) 19, 44, 142
MboII GAAGA 3 cut(s) 171, 174, 882
MfeI CAATTG 2 cut(s) 215, 489
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 4 cut(s) 29, 103, 261, 341
MlsI TGGCCA 2 cut(s) 612, 906
MluCI AATT 4 cut(s) 50, 215, 293, 489
MluNI TGGCCA 2 cut(s) 612, 906
MlyI GAGTC 4 cut(s) 91, 192, 232, 378
MmeI TCCRAC 4 cut(s) 59, 210, 608, 721
MnlI CCTC 9 cut(s) 64, 161, 187, 201, 238, 271, 424, 487, 610
Mox20I TGGCCA 2 cut(s) 612, 906
Mph1103I ATGCAT 2 cut(s) 544, 813
MroXI GAANNNNTTC 1 cut(s) 170
MscI TGGCCA 2 cut(s) 612, 906
MseI TTAA 4 cut(s) 408, 659, 693, 782
Msp20I TGGCCA 2 cut(s) 612, 906
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 4 cut(s) 134, 227, 269, 687
MunI CAATTG 2 cut(s) 215, 489
Mva1269I GAATGC 2 cut(s) 542, 813
MvaI CCWGG 4 cut(s) 134, 227, 269, 687
MwoI GCNNNNNNNGC 5 cut(s) 217, 345, 494, 509, 920
NcoI CCATGG 1 cut(s) 456
NdeII GATC 3 cut(s) 19, 44, 142
NlaIII CATG 5 cut(s) 364, 460, 468, 671, 815
NlaIV GGNNCC 7 cut(s) 117, 212, 339, 376, 417, 567, 735
NmuCI GTSAC 2 cut(s) 235, 593
NsiI ATGCAT 2 cut(s) 544, 813
NspI RCATGY 1 cut(s) 364
PasI CCCWGGG 1 cut(s) 686
PciI ACATGT 1 cut(s) 360
PcsI WCGNNNNNNNCGW 1 cut(s) 962
PctI GAATGC 2 cut(s) 542, 813
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 4 cut(s) 38, 461, 681, 953
PkrI GCNGC 4 cut(s) 68, 321, 514, 561
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 4 cut(s) 90, 192, 232, 377
PpsI GAGTC 4 cut(s) 90, 192, 232, 377
PpuMI RGGWCCY 1 cut(s) 115
PscI ACATGT 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 132, 225, 267, 685
PspGI CCWGG 4 cut(s) 132, 225, 267, 685
PspN4I GGNNCC 7 cut(s) 117, 212, 339, 376, 417, 567, 735
PspOMI GGGCCC 1 cut(s) 337
PspPI GGNCC 7 cut(s) 115, 130, 210, 337, 338, 374, 566
PspPPI RGGWCCY 1 cut(s) 115
PsuI RGATCY 1 cut(s) 19
PvuI CGATCG 1 cut(s) 145
RsaI GTAC 4 cut(s) 127, 426, 473, 622
RsaNI GTAC 4 cut(s) 126, 425, 472, 621
SacI GAGCTC 1 cut(s) 103
SaqAI TTAA 4 cut(s) 408, 659, 693, 782
SatI GCNGC 4 cut(s) 67, 320, 513, 560
Sau3AI GATC 3 cut(s) 19, 44, 142
Sau96I GGNCC 7 cut(s) 115, 130, 210, 337, 338, 374, 566
SchI GAGTC 4 cut(s) 91, 192, 232, 378
ScrFI CCNGG 4 cut(s) 134, 227, 269, 687
SduI GDGCHC 4 cut(s) 29, 103, 261, 341
SexAI ACCWGGT 1 cut(s) 225
SfaNI GCATC 5 cut(s) 529, 832, 882, 910, 923
SinI GGWCC 3 cut(s) 115, 374, 566
Sse9I AATT 4 cut(s) 50, 215, 293, 489
SsiI CCGC 6 cut(s) 11, 66, 394, 413, 562, 977
SspMI CTAG 2 cut(s) 578, 854
SstI GAGCTC 1 cut(s) 103
StyD4I CCNGG 4 cut(s) 132, 225, 267, 685
StyI CCWWGG 2 cut(s) 333, 456
TaaI ACNGT 1 cut(s) 625
TaqI TCGA 1 cut(s) 153
TaqII GACCGA 1 cut(s) 362
TasI AATT 4 cut(s) 50, 215, 293, 489
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 4 cut(s) 38, 461, 681, 953
Tru1I TTAA 4 cut(s) 408, 659, 693, 782
Tru9I TTAA 4 cut(s) 408, 659, 693, 782
TseFI GTSAC 2 cut(s) 235, 593
TseI GCWGC 3 cut(s) 319, 512, 559
Tsp45I GTSAC 2 cut(s) 235, 593
TspDTI ATGAA 3 cut(s) 453, 656, 828
TspGWI ACGGA 1 cut(s) 1006
VpaK11BI GGWCC 3 cut(s) 115, 374, 566
XceI RCATGY 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 578, 854
Zsp2I ATGCAT 2 cut(s) 544, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.