RLG00000030763
ERF Family

and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009235
Physical Location & Seq
Reverse (-)
67015700 .. 67017993
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030763

Sequence Viewer

Length: 1068 bp
ATGGAGCTGGCGGTGATAGATCTGAGCCCCTACTTGGAATCGGGATCGGAATCGGAATCGGGCCAATTGAAGGAGCTGTGCGGCGAGGTCAGTCGGAGTCTCAGAGAAACCGGAGCTCTCTTAGTGAAGGACCCTCGATGTACCGCCCAGGACAACGATCGCTTCCTCGATATGATGGAGAACTACTTCGCCTCTTCTTCCGACTTCAAGCGACTCCACGAGAGGCCCCAATTGCATTACCAGGTCGGCGTGACTCCCGAGGGCGTGGAAGTGCCCAGAAGCCTGGTTGATGAGGAAATGCAGGAGAAATTGAAGCAAATGCCGAAACAAATGCAGCCTTCCACTCCCAAAGGCCCTGATTGCAAATGGCGATACATGTGGAGAGTCGGTCCCAGACCATCCAAAACCCGCTTTCAGGAACTTAATGCGGAGCCAGTTGTACCTGAGGGTTTTCCTGAGTGGAAAGAAACCATGGATTCATGGGGGTACAAGATGATTTCGGCAATTGAGGCTGTTGCTGAAATGGCTGCCATTGGATTTGGTTTGCCAAAGGATGCATTCACTTCCCTTATGCAGCAGGGACCACACCTTCTAGCTCCAACTGGAAGTGACCTTCAGCGTTATGGCCAAGAGGGTACTGTCTTTGCAGGATATCACTATGACCTGAACTTTTTAACTATTCATGGCAGAAGTAGATTCCCAGGGCTTAATATCTGGCTAAGAAATGGGCAAAAAGTTGAAGTGAAGGTTCCTGTCGGATGTCTGCTTATTCAGACTGGAAAGCAGATAGAATGGTTAACGGGTGGAGATTGCATAGCTGGAATGCATGAAGTAGTTGTAACAAACAGGACAACTGATGCAATCAAACTAGCAAGGGAGCAAAATCGCATCTTATGGAGAGTATCTTCTACTTTGTTTGGCCACATAGCATCTGATGCTGTCTTGAAGCCCTTAGGCCACTTTGCCGATTCGCCACTTGCGAGTAAATATCCGCTCATTTATGCAGGGGAGTTTGTTGAACAAGAGCTTTCCGTAATCAATCTCAAAGGAAACAAAGGACAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIOX_N

Protein Analysis

356

Amino Acids

39.83

Weight (kDa)

5.84

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 994
AciI CCGC 6 cut(s) 11, 81, 144, 409, 428, 992
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 2 cut(s) 625, 919
AcuI CTGAAG 1 cut(s) 599
AfaI GTAC 4 cut(s) 142, 441, 488, 637
AfiI CCNNNNNNNGG 3 cut(s) 34, 70, 415
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 6 cut(s) 70, 208, 313, 740, 946, 1019
AjnI CCWGG 4 cut(s) 147, 240, 282, 700
AluBI AGCT 6 cut(s) 7, 76, 116, 596, 818, 1027
AluI AGCT 6 cut(s) 7, 76, 116, 596, 818, 1027
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 6 cut(s) 61, 224, 352, 625, 919, 955
ApeKI GCWGC 3 cut(s) 334, 527, 574
Asp700I GAANNNNTTC 1 cut(s) 185
AspS9I GGNCC 6 cut(s) 61, 130, 225, 353, 389, 581
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 3 cut(s) 130, 389, 581
AxyI CCTNAGG 2 cut(s) 444, 952
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 2 cut(s) 627, 921
BanII GRGCYC 2 cut(s) 29, 118
BauI CACGAG 1 cut(s) 218
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 346, 514, 586
BccI CCATC 2 cut(s) 169, 406
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 4 cut(s) 149, 242, 284, 702
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 2 cut(s) 593, 869
BglII AGATCT 1 cut(s) 19
BisI GCNGC 4 cut(s) 82, 335, 528, 575
BlsI GCNGC 4 cut(s) 83, 336, 529, 576
Bme1390I CCNGG 4 cut(s) 149, 242, 284, 702
Bme18I GGWCC 3 cut(s) 130, 389, 581
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 6 cut(s) 61, 130, 225, 353, 389, 581
BmiI GGNNCC 6 cut(s) 132, 227, 391, 432, 582, 750
BmrFI CCNGG 4 cut(s) 149, 242, 284, 702
BmsI GCATC 5 cut(s) 544, 847, 897, 925, 938
BsaBI GATNNNNATC 1 cut(s) 49
BsaJI CCNNGG 5 cut(s) 147, 258, 471, 700, 701
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 70, 415
Bse1I ACTGG 3 cut(s) 434, 607, 781
Bse21I CCTNAGG 2 cut(s) 444, 952
Bse8I GATNNNNATC 1 cut(s) 49
BseBI CCWGG 4 cut(s) 149, 242, 284, 702
BseDI CCNNGG 5 cut(s) 147, 258, 471, 700, 701
BseGI GGATG 3 cut(s) 398, 559, 764
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 3 cut(s) 34, 70, 415
BseMII CTCAG 4 cut(s) 14, 115, 435, 447
BseNI ACTGG 3 cut(s) 434, 607, 781
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 3 cut(s) 346, 514, 586
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 6 cut(s) 63, 226, 354, 627, 921, 957
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 2 cut(s) 375, 594
BslI CCNNNNNNNGG 3 cut(s) 34, 70, 415
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 2 cut(s) 375, 594
BsmI GAATGC 2 cut(s) 557, 828
BsnI GGCC 6 cut(s) 63, 226, 354, 627, 921, 957
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 3 cut(s) 29, 118, 276
Bsp143I GATC 3 cut(s) 19, 44, 157
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 6 cut(s) 11, 81, 144, 409, 428, 992
BspANI GGCC 6 cut(s) 63, 226, 354, 627, 921, 957
BspCNI CTCAG 4 cut(s) 15, 114, 436, 448
BspLI GGNNCC 6 cut(s) 132, 227, 391, 432, 582, 750
BspPI GGATC 1 cut(s) 52
BsrBI CCGCTC 1 cut(s) 994
BsrI ACTGG 3 cut(s) 434, 607, 781
BssECI CCNNGG 5 cut(s) 147, 258, 471, 700, 701
BssMI GATC 3 cut(s) 19, 44, 157
BssSI CACGAG 1 cut(s) 218
BssT1I CCWWGG 1 cut(s) 471
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 4 cut(s) 149, 242, 284, 702
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 199
BstAPI GCANNNNNTGC 1 cut(s) 935
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 7 cut(s) 23, 101, 121, 444, 456, 719, 952
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 3 cut(s) 398, 559, 764
BstKTI GATC 3 cut(s) 22, 47, 160
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 3 cut(s) 19, 44, 157
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 5 cut(s) 232, 360, 509, 524, 935
BstNI CCWGG 4 cut(s) 149, 242, 284, 702
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 4 cut(s) 147, 240, 282, 700
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 346, 514, 586
BstX2I RGATCY 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 19
Bsu36I CCTNAGG 2 cut(s) 444, 952
BsuRI GGCC 6 cut(s) 63, 226, 354, 627, 921, 957
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 3 cut(s) 398, 559, 764
Cac8I GCNNGC 1 cut(s) 9
Cfr13I GGNCC 6 cut(s) 61, 130, 225, 353, 389, 581
CsiI ACCWGGT 1 cut(s) 240
Csp6I GTAC 4 cut(s) 141, 440, 487, 636
CviAII CATG 5 cut(s) 376, 472, 480, 683, 827
CviQI GTAC 4 cut(s) 141, 440, 487, 636
DdeI CTNAG 7 cut(s) 23, 101, 121, 444, 456, 719, 952
DpnI GATC 3 cut(s) 21, 46, 159
DpnII GATC 3 cut(s) 19, 44, 157
EaeI YGGCCR 2 cut(s) 625, 919
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
Ecl136II GAGCTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 2 cut(s) 29, 118
Eco32I GATATC 1 cut(s) 653
Eco47I GGWCC 3 cut(s) 130, 389, 581
Eco53kI GAGCTC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 599
Eco81I CCTNAGG 2 cut(s) 444, 952
Eco88I CYCGRG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 116
EcoO109I RGGNCCY 3 cut(s) 130, 225, 353
EcoRII CCWGG 4 cut(s) 147, 240, 282, 700
EcoRV GATATC 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 2 cut(s) 559, 828
EcoT38I GRGCYC 2 cut(s) 29, 118
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 5 cut(s) 379, 475, 483, 686, 830
FalI AAGNNNNNCTT 1 cut(s) 1047
FaqI GGGAC 2 cut(s) 375, 594
FatI CATG 5 cut(s) 375, 471, 479, 682, 826
FauI CCCGC 1 cut(s) 416
Fnu4HI GCNGC 4 cut(s) 82, 335, 528, 575
FokI GGATG 3 cut(s) 385, 566, 771
FriOI GRGCYC 2 cut(s) 29, 118
Fsp4HI GCNGC 4 cut(s) 82, 335, 528, 575
FspBI CTAG 2 cut(s) 593, 869
GluI GCNGC 4 cut(s) 82, 335, 528, 575
HaeIII GGCC 6 cut(s) 63, 226, 354, 627, 921, 957
HapII CCGG 1 cut(s) 111
Hin1II CATG 5 cut(s) 379, 475, 483, 686, 830
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HpaI GTTAAC 1 cut(s) 798
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 798
Hpy188I TCNGA 9 cut(s) 24, 49, 55, 96, 104, 202, 758, 774, 934
Hpy188III TCNNGA 5 cut(s) 42, 257, 416, 455, 943
Hpy8I GTNNAC 1 cut(s) 798
HpyAV CCTTC 6 cut(s) 64, 121, 348, 599, 623, 739
HpyCH4III ACNGT 1 cut(s) 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 232, 360, 509, 524, 935
HpyF3I CTNAG 7 cut(s) 23, 101, 121, 444, 456, 719, 952
Hsp92II CATG 5 cut(s) 379, 475, 483, 686, 830
KspAI GTTAAC 1 cut(s) 798
Kzo9I GATC 3 cut(s) 19, 44, 157
LmnI GCTCC 6 cut(s) 4, 73, 113, 430, 601, 877
Lsp1109I GCAGC 3 cut(s) 346, 514, 586
LweI GCATC 5 cut(s) 544, 847, 897, 925, 938
MabI ACCWGGT 1 cut(s) 240
MaeI CTAG 2 cut(s) 593, 869
MaeIII GTNAC 3 cut(s) 250, 608, 838
MalI GATC 3 cut(s) 21, 46, 159
MbiI CCGCTC 1 cut(s) 994
MboI GATC 3 cut(s) 19, 44, 157
MboII GAAGA 3 cut(s) 186, 189, 897
MfeI CAATTG 3 cut(s) 65, 230, 504
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 3 cut(s) 29, 118, 276
MlsI TGGCCA 2 cut(s) 627, 921
MluCI AATT 4 cut(s) 65, 230, 308, 504
MluNI TGGCCA 2 cut(s) 627, 921
MlyI GAGTC 4 cut(s) 106, 207, 247, 393
MmeI TCCRAC 4 cut(s) 74, 225, 623, 736
Mox20I TGGCCA 2 cut(s) 627, 921
Mph1103I ATGCAT 2 cut(s) 559, 828
MroXI GAANNNNTTC 1 cut(s) 185
MscI TGGCCA 2 cut(s) 627, 921
MseI TTAA 4 cut(s) 423, 674, 708, 797
Msp20I TGGCCA 2 cut(s) 627, 921
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 4 cut(s) 149, 242, 284, 702
MunI CAATTG 3 cut(s) 65, 230, 504
Mva1269I GAATGC 2 cut(s) 557, 828
MvaI CCWGG 4 cut(s) 149, 242, 284, 702
MwoI GCNNNNNNNGC 5 cut(s) 232, 360, 509, 524, 935
NcoI CCATGG 1 cut(s) 471
NdeII GATC 3 cut(s) 19, 44, 157
NlaIII CATG 5 cut(s) 379, 475, 483, 686, 830
NlaIV GGNNCC 6 cut(s) 132, 227, 391, 432, 582, 750
NmuCI GTSAC 2 cut(s) 250, 608
NsiI ATGCAT 2 cut(s) 559, 828
NspI RCATGY 1 cut(s) 379
PasI CCCWGGG 1 cut(s) 701
PciI ACATGT 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 977
PctI GAATGC 2 cut(s) 557, 828
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 6 cut(s) 38, 50, 56, 476, 696, 968
PkrI GCNGC 4 cut(s) 83, 336, 529, 576
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 4 cut(s) 105, 207, 247, 392
PpsI GAGTC 4 cut(s) 105, 207, 247, 392
PpuMI RGGWCCY 1 cut(s) 130
PscI ACATGT 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 118
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 4 cut(s) 147, 240, 282, 700
PspGI CCWGG 4 cut(s) 147, 240, 282, 700
PspN4I GGNNCC 6 cut(s) 132, 227, 391, 432, 582, 750
PspPI GGNCC 6 cut(s) 61, 130, 225, 353, 389, 581
PspPPI RGGWCCY 1 cut(s) 130
PsuI RGATCY 1 cut(s) 19
PvuI CGATCG 1 cut(s) 160
RsaI GTAC 4 cut(s) 142, 441, 488, 637
RsaNI GTAC 4 cut(s) 141, 440, 487, 636
SacI GAGCTC 1 cut(s) 118
SaqAI TTAA 4 cut(s) 423, 674, 708, 797
SatI GCNGC 4 cut(s) 82, 335, 528, 575
Sau3AI GATC 3 cut(s) 19, 44, 157
Sau96I GGNCC 6 cut(s) 61, 130, 225, 353, 389, 581
SchI GAGTC 4 cut(s) 106, 207, 247, 393
ScrFI CCNGG 4 cut(s) 149, 242, 284, 702
SduI GDGCHC 3 cut(s) 29, 118, 276
SexAI ACCWGGT 1 cut(s) 240
SfaNI GCATC 5 cut(s) 544, 847, 897, 925, 938
SinI GGWCC 3 cut(s) 130, 389, 581
Sse9I AATT 4 cut(s) 65, 230, 308, 504
SsiI CCGC 6 cut(s) 11, 81, 144, 409, 428, 992
SspMI CTAG 2 cut(s) 593, 869
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 4 cut(s) 147, 240, 282, 700
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 640
TaqI TCGA 2 cut(s) 136, 168
TaqII GACCGA 1 cut(s) 377
TasI AATT 4 cut(s) 65, 230, 308, 504
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 6 cut(s) 38, 50, 56, 476, 696, 968
Tru1I TTAA 4 cut(s) 423, 674, 708, 797
Tru9I TTAA 4 cut(s) 423, 674, 708, 797
TseFI GTSAC 2 cut(s) 250, 608
TseI GCWGC 3 cut(s) 334, 527, 574
Tsp45I GTSAC 2 cut(s) 250, 608
TspDTI ATGAA 3 cut(s) 468, 671, 843
TspGWI ACGGA 1 cut(s) 1021
VpaK11BI GGWCC 3 cut(s) 130, 389, 581
XceI RCATGY 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 2 cut(s) 593, 869
Zsp2I ATGCAT 2 cut(s) 559, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.