pycom07g00470
ERF Family

and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009235
Physical Location & Seq
Forward (+)
456363 .. 458591
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00470.3

Sequence Viewer

Length: 765 bp
ATGCAGGAGAAATTGAAAGAAATGCCCAAAGAGTTCCAGCCATCCATCCCCGAAGGGGCAGATCGGAAATGGCGATACATGTGGAGGGTGGGTCCTCGACCCTCGAAAACCCGCTTTCAGGAACTTAATGCAGAGCCAGTCGTACCCGAGGGGTTTCCTGAATGGAAAGATACCATGGATTCATGGGGTTACAAGATGATTTCCGCAATAGAGGCTGTTGCTGAAATGGCTGCCATTGGATTTGGCTTGCCAAAGGATGCATTCACTTCTCTTATGAAGCAGGGGCCACACCTTCTAGCTCCAACAGGAAGTGACCTTCGGCGTTATGGCCAAGAGGGTACTGTGTTTGCAGGATATCACTATGACCTGAACTTTCTAACTATTCATGGCAGAAGTAGATTTCCCGGTCTGAACATTTGGTTAAAAAATGGGCAAAAAGTTGAAGTCAAGGTTCCTGTAGGATGTCTTCTCATTCAGACCGGGAAGCAGATAGAATGGTTGACTGCAGGTGATTGCATAGCTGGAATGCATGAAGTTATCGTAACAAACAGGACAATTGATGCAATCAAACTTGCAACAGAGCAAAGTCGCTTACTATGGAGAGTATCTTCAACATTGTTTGCACACGTAGCATCTGATGCTGTGTTGAAGCCCTTAGGTCACTTTGCAGAATCCCCGCTTACGAGTAAATACCCGCCCATTTATGCAGGAGAGTTTGTTGAACAAGAGCTTGCAGTAATCAATCTCAAAGGCAACAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIOX_N

Protein Analysis

255

Amino Acids

28.53

Weight (kDa)

7.77

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 497
AciI CCGC 4 cut(s) 112, 204, 677, 695
AcoI YGGCCR 1 cut(s) 328
AfaI GTAC 2 cut(s) 144, 340
AfiI CCNNNNNNNGG 2 cut(s) 55, 118
AflIII ACRYGT 2 cut(s) 78, 625
AgsI TTSAA 5 cut(s) 16, 443, 612, 649, 722
AluBI AGCT 3 cut(s) 299, 521, 730
AluI AGCT 3 cut(s) 299, 521, 730
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 2 cut(s) 284, 328
ApeKI GCWGC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 92, 284
AsuC2I CCSGG 2 cut(s) 405, 481
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 655
BalI TGGCCA 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 450, 482
BbsI GAAGAC 1 cut(s) 458
BbvI GCAGC 1 cut(s) 217
BccI CCATC 2 cut(s) 49, 53
BcnI CCSGG 2 cut(s) 405, 481
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 456, 504
BfuAI ACCTGC 1 cut(s) 497
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 2 cut(s) 405, 481
Bme18I GGWCC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 92, 284
BmiI GGNNCC 3 cut(s) 93, 285, 453
BmrFI CCNGG 2 cut(s) 405, 481
BmsI GCATC 4 cut(s) 247, 550, 628, 641
BpiI GAAGAC 1 cut(s) 458
BpuMI CCSGG 2 cut(s) 405, 481
BsaAI YACGTR 1 cut(s) 628
BsaJI CCNNGG 2 cut(s) 147, 174
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 702, 732
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 118
Bse1I ACTGG 1 cut(s) 137
Bse21I CCTNAGG 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 147, 174
BseGI GGATG 4 cut(s) 41, 45, 262, 467
BseLI CCNNNNNNNGG 2 cut(s) 55, 118
BseNI ACTGG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 2 cut(s) 286, 330
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 2 cut(s) 405, 480
BslI CCNNNNNNNGG 2 cut(s) 55, 118
BsmI GAATGC 2 cut(s) 260, 531
BsnI GGCC 2 cut(s) 286, 330
BsoBI CYCGRG 1 cut(s) 146
Bsp143I GATC 1 cut(s) 61
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 4 cut(s) 112, 204, 677, 695
BspANI GGCC 2 cut(s) 286, 330
BspLI GGNNCC 3 cut(s) 93, 285, 453
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 147, 174
BssMI GATC 1 cut(s) 61
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 638
BstBAI YACGTR 1 cut(s) 628
BstC8I GCNNGC 2 cut(s) 248, 732
BstDEI CTNAG 1 cut(s) 655
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 4 cut(s) 41, 45, 262, 467
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 4 cut(s) 212, 227, 629, 638
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 403, 479
BstSFI CTRYAG 2 cut(s) 456, 504
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 458
Bsu36I CCTNAGG 1 cut(s) 655
BsuRI GGCC 2 cut(s) 286, 330
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 4 cut(s) 41, 45, 262, 467
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 2 cut(s) 248, 732
Cfr13I GGNCC 2 cut(s) 92, 284
Csp6I GTAC 2 cut(s) 143, 339
CviAII CATG 5 cut(s) 79, 175, 183, 386, 530
CviQI GTAC 2 cut(s) 143, 339
DdeI CTNAG 1 cut(s) 655
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 174
Eco32I GATATC 1 cut(s) 356
Eco47I GGWCC 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 655
Eco88I CYCGRG 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 92
EcoRV GATATC 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 174
EcoT22I ATGCAT 2 cut(s) 262, 531
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 5 cut(s) 82, 178, 186, 389, 533
FatI CATG 5 cut(s) 78, 174, 182, 385, 529
FauI CCCGC 3 cut(s) 119, 684, 702
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 4 cut(s) 28, 32, 269, 474
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 1 cut(s) 296
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 286, 330
HapII CCGG 2 cut(s) 405, 480
Hin1II CATG 5 cut(s) 82, 178, 186, 389, 533
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HinfI GANTC 2 cut(s) 179, 671
HpaII CCGG 2 cut(s) 405, 480
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 1 cut(s) 501
Hpy188I TCNGA 4 cut(s) 66, 411, 477, 637
Hpy188III TCNNGA 2 cut(s) 119, 158
Hpy8I GTNNAC 1 cut(s) 501
HpyAV CCTTC 3 cut(s) 47, 302, 326
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 1 cut(s) 627
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 227, 629, 638
HpyF3I CTNAG 1 cut(s) 655
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 5 cut(s) 82, 178, 186, 389, 533
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 304
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 4 cut(s) 247, 550, 628, 641
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 4 cut(s) 188, 311, 541, 659
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 2 cut(s) 458, 600
MfeI CAATTG 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 2 cut(s) 11, 555
MluNI TGGCCA 1 cut(s) 330
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 6 cut(s) 78, 105, 112, 142, 205, 328
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 2 cut(s) 262, 531
MscI TGGCCA 1 cut(s) 330
MseI TTAA 2 cut(s) 126, 422
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 2 cut(s) 405, 480
MspR9I CCNGG 2 cut(s) 405, 481
MunI CAATTG 1 cut(s) 555
Mva1269I GAATGC 2 cut(s) 260, 531
MwoI GCNNNNNNNGC 4 cut(s) 212, 227, 629, 638
NciI CCSGG 2 cut(s) 405, 481
NcoI CCATGG 1 cut(s) 174
NdeII GATC 1 cut(s) 61
NlaIII CATG 5 cut(s) 82, 178, 186, 389, 533
NlaIV GGNNCC 3 cut(s) 93, 285, 453
NmuCI GTSAC 2 cut(s) 311, 659
NsiI ATGCAT 2 cut(s) 262, 531
NspI RCATGY 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 497
PciI ACATGT 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 70
PctI GAATGC 2 cut(s) 260, 531
PfeI GAWTC 2 cut(s) 179, 671
PkrI GCNGC 1 cut(s) 232
Ppu21I YACGTR 1 cut(s) 628
PpuMI RGGWCCY 1 cut(s) 92
PscI ACATGT 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 92
PspN4I GGNNCC 3 cut(s) 93, 285, 453
PspPI GGNCC 2 cut(s) 92, 284
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 1 cut(s) 508
RsaI GTAC 2 cut(s) 144, 340
RsaNI GTAC 2 cut(s) 143, 339
SaqAI TTAA 2 cut(s) 126, 422
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 2 cut(s) 92, 284
ScrFI CCNGG 2 cut(s) 405, 481
SfaNI GCATC 4 cut(s) 247, 550, 628, 641
SfcI CTRYAG 2 cut(s) 456, 504
SinI GGWCC 1 cut(s) 92
Sse9I AATT 2 cut(s) 11, 555
SsiI CCGC 4 cut(s) 112, 204, 677, 695
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 403, 479
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 1 cut(s) 630
TaqI TCGA 2 cut(s) 97, 104
TasI AATT 2 cut(s) 11, 555
TfiI GAWTC 2 cut(s) 179, 671
Tru1I TTAA 2 cut(s) 126, 422
Tru9I TTAA 2 cut(s) 126, 422
TseFI GTSAC 2 cut(s) 311, 659
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 2 cut(s) 311, 659
TspDTI ATGAA 4 cut(s) 171, 290, 374, 546
VpaK11BI GGWCC 1 cut(s) 92
XceI RCATGY 1 cut(s) 82
XspI CTAG 1 cut(s) 296
Zsp2I ATGCAT 2 cut(s) 262, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.