Rroxscaffold_4G00331870
ERF Family

and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009235
Physical Location & Seq
Reverse (-)
65852681 .. 65856266
3586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331870.1

Sequence Viewer

Length: 1158 bp
ATGGAGCTGGCGGTGATAGATCTGAGCCCCTACTTGGAATCGGGATCGGAATCATCACCGGAATCGGGCCAATGGAAGGAGCTGTGCGGCGAGGTCAGTCGGAGTCTCAGAGAAACCGGAGCTCTCTTAGTCAAGGACCCTCGATGTACGGCCCAGGACAACGATCGCTTCCTCGATATGATGGAGAACTACTTCGCCTCTTCTTCCGACTTCAAGCGACTCCACGAGAGGCCCCAATTGCATTACCAGGTCGGTGTGACTCCCGAGGGCGTGGAAGTGCCCAGAAGCCTGGTTGATGAGGAAATGCAGGAGAAATTGAAGCAAATGCCGAAGCAAGTGCAGCCTTCCACTCCCAAGGGCCCTGATTGCAAATGGCGATACATGTGGAGAGTCGGTCCCAGACCATCCAAAACCCGCTTTCAGGAGCTTAATGCGGAGCCAGTTGTGCCTGAGGGTTTTCCTGAGTGGAAAGAAACCATGGATTCATGGGGGTACAAGATGATTTCGGCAATTGAGGCTGTTGCTGAAATGGCTGCCATTGGATTTGGTTTGCCAAAGGATGCATTCACTTCCCTTATGCAGCAGGGACCACACCTTCTAGCTCCAACTGGAAGTGACCTTCAGCGTTATGGCCAAGAGGGTACTGTCTTTGCAGGATATCACTATGACCTGAACTTTTTAACTATTCATGGCAGAAGTAGATTCCCAGGGCTTAATATCTGGCTAAGAAATGGGCAAAAAGTTGAAGTGAAGGTTCCTGTCGGATGTCTGCTTATTCAGACTGGAAAGCAGTTGTTTGGCCACATAGCATCTGATGCTGTCTTGAAGCCCTTAGGCCACTTTGCCGATTCGCCACTTGCCAGTAAATATCCGCCCATTTATGCAGGGGAGTTTGTTGAACAAGAGCTTTCCTCGCAGTTCAAATCGAAGATGAGGCACACTAGAAGCTCTGAGGTTCTTGCAACTTTTGTGCTTATACCAGCAGTGGTTCCTTTTAGCCCCATCCCACCAAGTCAGGCTTGCTGCACTGCACTCAGAGCTTTAGGCCAGTCTTGCCTTTGTGTGCTCGTCAATGGCCCTCCGATCTCTGGCGTTGATCGCAACATGGCAATTCAGCTCCCAGAGAAGTGCAATGCCAACTTTGAACCATGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIOX_N

Protein Analysis

385

Amino Acids

42.83

Weight (kDa)

5.97

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 336 - 384 1.7e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 11, 87, 415, 434, 872
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 2 cut(s) 631, 799
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 3 cut(s) 148, 494, 643
AfiI CCNNNNNNNGG 5 cut(s) 34, 65, 76, 421, 1088
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 7 cut(s) 214, 319, 746, 826, 899, 922, 1145
AjnI CCWGG 4 cut(s) 153, 246, 288, 706
AluBI AGCT 9 cut(s) 7, 82, 122, 427, 602, 907, 948, 1040, 1117
AluI AGCT 9 cut(s) 7, 82, 122, 427, 602, 907, 948, 1040, 1117
Alw21I GWGCWC 2 cut(s) 124, 1068
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 9 cut(s) 67, 150, 230, 358, 631, 799, 835, 1045, 1075
ApaI GGGCCC 1 cut(s) 362
ApeKI GCWGC 4 cut(s) 340, 533, 580, 1023
Asp700I GAANNNNTTC 1 cut(s) 191
AspS9I GGNCC 9 cut(s) 67, 136, 151, 231, 358, 359, 395, 587, 1076
AsuHPI GGTGA 2 cut(s) 25, 48
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 3 cut(s) 136, 395, 587
AxyI CCTNAGG 2 cut(s) 450, 832
BaeGI GKGCMC 2 cut(s) 282, 362
BalI TGGCCA 2 cut(s) 633, 801
BanII GRGCYC 3 cut(s) 29, 124, 362
BauI CACGAG 1 cut(s) 224
Bbv12I GWGCWC 2 cut(s) 124, 1068
BbvI GCAGC 4 cut(s) 352, 520, 592, 1010
BccI CCATC 3 cut(s) 175, 412, 1010
BceAI ACGGC 1 cut(s) 165
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 4 cut(s) 155, 248, 290, 708
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 599, 942
BglII AGATCT 1 cut(s) 19
BisI GCNGC 5 cut(s) 88, 341, 534, 581, 1024
BlsI GCNGC 5 cut(s) 89, 342, 535, 582, 1025
Bme1390I CCNGG 4 cut(s) 155, 248, 290, 708
Bme18I GGWCC 3 cut(s) 136, 395, 587
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 9 cut(s) 67, 136, 151, 231, 358, 359, 395, 587, 1076
BmiI GGNNCC 8 cut(s) 138, 233, 360, 397, 438, 588, 756, 990
BmrFI CCNGG 4 cut(s) 155, 248, 290, 708
BmsI GCATC 3 cut(s) 550, 805, 818
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BsaBI GATNNNNATC 1 cut(s) 49
BsaJI CCNNGG 6 cut(s) 153, 264, 354, 477, 706, 707
BsaWI WCCGGW 2 cut(s) 58, 116
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 65, 76, 421, 1088
Bse1I ACTGG 5 cut(s) 440, 613, 787, 861, 1048
Bse21I CCTNAGG 2 cut(s) 450, 832
Bse3DI GCAATG 1 cut(s) 1138
Bse8I GATNNNNATC 1 cut(s) 49
BseBI CCWGG 4 cut(s) 155, 248, 290, 708
BseDI CCNNGG 6 cut(s) 153, 264, 354, 477, 706, 707
BseGI GGATG 4 cut(s) 404, 565, 770, 1002
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 5 cut(s) 34, 65, 76, 421, 1088
BseMI GCAATG 1 cut(s) 1138
BseMII CTCAG 6 cut(s) 14, 121, 441, 453, 942, 1048
BseNI ACTGG 5 cut(s) 440, 613, 787, 861, 1048
BseSI GKGCMC 2 cut(s) 282, 362
BseXI GCAGC 4 cut(s) 352, 520, 592, 1010
BsgI GTGCAG 3 cut(s) 359, 1009, 1014
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 9 cut(s) 69, 152, 232, 360, 633, 801, 837, 1047, 1077
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 2 cut(s) 124, 1068
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 2 cut(s) 59, 117
BslFI GGGAC 2 cut(s) 381, 600
BslI CCNNNNNNNGG 5 cut(s) 34, 65, 76, 421, 1088
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 2 cut(s) 381, 600
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 9 cut(s) 69, 152, 232, 360, 633, 801, 837, 1047, 1077
BsoBI CYCGRG 1 cut(s) 263
Bsp120I GGGCCC 1 cut(s) 358
Bsp1286I GDGCHC 5 cut(s) 29, 124, 282, 362, 1068
Bsp143I GATC 5 cut(s) 19, 44, 163, 1083, 1096
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 5 cut(s) 11, 87, 415, 434, 872
BspANI GGCC 9 cut(s) 69, 152, 232, 360, 633, 801, 837, 1047, 1077
BspCNI CTCAG 6 cut(s) 15, 120, 442, 454, 943, 1047
BspLI GGNNCC 8 cut(s) 138, 233, 360, 397, 438, 588, 756, 990
BspPI GGATC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 1138
BsrI ACTGG 5 cut(s) 440, 613, 787, 861, 1048
BssECI CCNNGG 6 cut(s) 153, 264, 354, 477, 706, 707
BssMI GATC 5 cut(s) 19, 44, 163, 1083, 1096
BssSI CACGAG 1 cut(s) 224
BssT1I CCWWGG 2 cut(s) 354, 477
Bst2BI CACGAG 1 cut(s) 224
Bst2UI CCWGG 4 cut(s) 155, 248, 290, 708
Bst4CI ACNGT 1 cut(s) 646
Bst6I CTCTTC 1 cut(s) 205
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 2 cut(s) 9, 1021
BstDEI CTNAG 9 cut(s) 23, 107, 127, 450, 462, 725, 832, 951, 1034
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 404, 565, 770, 1002
BstKTI GATC 5 cut(s) 22, 47, 166, 1086, 1099
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 5 cut(s) 19, 44, 163, 1083, 1096
BstMCI CGRYCG 1 cut(s) 166
BstNI CCWGG 4 cut(s) 155, 248, 290, 708
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 4 cut(s) 153, 246, 288, 706
BstSLI GKGCMC 2 cut(s) 282, 362
BstV1I GCAGC 4 cut(s) 352, 520, 592, 1010
BstX2I RGATCY 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 1 cut(s) 19
Bsu36I CCTNAGG 2 cut(s) 450, 832
BsuRI GGCC 9 cut(s) 69, 152, 232, 360, 633, 801, 837, 1047, 1077
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 4 cut(s) 404, 565, 770, 1002
BtsI GCAGTG 2 cut(s) 990, 1026
BtsIMutI CAGTG 2 cut(s) 990, 1026
Cac8I GCNNGC 2 cut(s) 9, 1021
Cfr13I GGNCC 9 cut(s) 67, 136, 151, 231, 358, 359, 395, 587, 1076
CsiI ACCWGGT 1 cut(s) 246
Csp6I GTAC 3 cut(s) 147, 493, 642
CviAII CATG 6 cut(s) 382, 478, 486, 689, 1105, 1149
CviQI GTAC 3 cut(s) 147, 493, 642
DdeI CTNAG 9 cut(s) 23, 107, 127, 450, 462, 725, 832, 951, 1034
DpnI GATC 5 cut(s) 21, 46, 165, 1085, 1098
DpnII GATC 5 cut(s) 19, 44, 163, 1083, 1096
EaeI YGGCCR 2 cut(s) 631, 799
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 861
Ecl136II GAGCTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 354, 477
Eco24I GRGCYC 3 cut(s) 29, 124, 362
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 3 cut(s) 136, 395, 587
Eco53kI GAGCTC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 605
Eco81I CCTNAGG 2 cut(s) 450, 832
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 122
EcoO109I RGGNCCY 4 cut(s) 136, 231, 358, 359
EcoRII CCWGG 4 cut(s) 153, 246, 288, 706
EcoRV GATATC 1 cut(s) 659
EcoT14I CCWWGG 2 cut(s) 354, 477
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 3 cut(s) 29, 124, 362
ErhI CCWWGG 2 cut(s) 354, 477
FaeI CATG 6 cut(s) 385, 481, 489, 692, 1108, 1152
FalI AAGNNNNNCTT 2 cut(s) 1003, 1035
FaqI GGGAC 2 cut(s) 381, 600
FatI CATG 6 cut(s) 381, 477, 485, 688, 1104, 1148
FauI CCCGC 1 cut(s) 422
Fnu4HI GCNGC 5 cut(s) 88, 341, 534, 581, 1024
FokI GGATG 4 cut(s) 391, 572, 777, 989
FriOI GRGCYC 3 cut(s) 29, 124, 362
Fsp4HI GCNGC 5 cut(s) 88, 341, 534, 581, 1024
FspBI CTAG 2 cut(s) 599, 942
GluI GCNGC 5 cut(s) 88, 341, 534, 581, 1024
HaeIII GGCC 9 cut(s) 69, 152, 232, 360, 633, 801, 837, 1047, 1077
HapII CCGG 2 cut(s) 59, 117
Hin1II CATG 6 cut(s) 385, 481, 489, 692, 1108, 1152
HpaII CCGG 2 cut(s) 59, 117
HphI GGTGA 2 cut(s) 25, 48
Hpy188III TCNNGA 5 cut(s) 42, 263, 422, 461, 823
HpyAV CCTTC 5 cut(s) 70, 354, 605, 629, 745
HpyCH4III ACNGT 1 cut(s) 646
HpyF3I CTNAG 9 cut(s) 23, 107, 127, 450, 462, 725, 832, 951, 1034
Hsp92II CATG 6 cut(s) 385, 481, 489, 692, 1108, 1152
Kzo9I GATC 5 cut(s) 19, 44, 163, 1083, 1096
LmnI GCTCC 7 cut(s) 4, 79, 119, 424, 436, 607, 1122
Lsp1109I GCAGC 4 cut(s) 352, 520, 592, 1010
LweI GCATC 3 cut(s) 550, 805, 818
MabI ACCWGGT 1 cut(s) 246
MaeI CTAG 2 cut(s) 599, 942
MaeIII GTNAC 3 cut(s) 256, 614, 1151
MalI GATC 5 cut(s) 21, 46, 165, 1085, 1098
MboI GATC 5 cut(s) 19, 44, 163, 1083, 1096
MboII GAAGA 3 cut(s) 192, 195, 940
MfeI CAATTG 2 cut(s) 236, 510
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 5 cut(s) 29, 124, 282, 362, 1068
MlsI TGGCCA 2 cut(s) 633, 801
MluCI AATT 4 cut(s) 236, 314, 510, 1110
MluNI TGGCCA 2 cut(s) 633, 801
MlyI GAGTC 4 cut(s) 112, 213, 253, 399
MmeI TCCRAC 4 cut(s) 80, 231, 629, 742
Mox20I TGGCCA 2 cut(s) 633, 801
Mph1103I ATGCAT 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 191
MscI TGGCCA 2 cut(s) 633, 801
MseI TTAA 3 cut(s) 429, 680, 714
Msp20I TGGCCA 2 cut(s) 633, 801
MspI CCGG 2 cut(s) 59, 117
MspR9I CCNGG 4 cut(s) 155, 248, 290, 708
MunI CAATTG 2 cut(s) 236, 510
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 4 cut(s) 155, 248, 290, 708
NcoI CCATGG 1 cut(s) 477
NdeII GATC 5 cut(s) 19, 44, 163, 1083, 1096
NlaIII CATG 6 cut(s) 385, 481, 489, 692, 1108, 1152
NlaIV GGNNCC 8 cut(s) 138, 233, 360, 397, 438, 588, 756, 990
NmuCI GTSAC 2 cut(s) 256, 614
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 385
PasI CCCWGGG 1 cut(s) 707
PciI ACATGT 1 cut(s) 381
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 6 cut(s) 38, 50, 62, 482, 702, 848
PkrI GCNGC 5 cut(s) 89, 342, 535, 582, 1025
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 4 cut(s) 111, 213, 253, 398
PpsI GAGTC 4 cut(s) 111, 213, 253, 398
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 4 cut(s) 153, 246, 288, 706
PspGI CCWGG 4 cut(s) 153, 246, 288, 706
PspN4I GGNNCC 8 cut(s) 138, 233, 360, 397, 438, 588, 756, 990
PspOMI GGGCCC 1 cut(s) 358
PspPI GGNCC 9 cut(s) 67, 136, 151, 231, 358, 359, 395, 587, 1076
PspPPI RGGWCCY 1 cut(s) 136
PsuI RGATCY 1 cut(s) 19
PvuI CGATCG 1 cut(s) 166
RsaI GTAC 3 cut(s) 148, 494, 643
RsaNI GTAC 3 cut(s) 147, 493, 642
SacI GAGCTC 1 cut(s) 124
SaqAI TTAA 3 cut(s) 429, 680, 714
SatI GCNGC 5 cut(s) 88, 341, 534, 581, 1024
Sau3AI GATC 5 cut(s) 19, 44, 163, 1083, 1096
Sau96I GGNCC 9 cut(s) 67, 136, 151, 231, 358, 359, 395, 587, 1076
SchI GAGTC 4 cut(s) 112, 213, 253, 399
ScrFI CCNGG 4 cut(s) 155, 248, 290, 708
SduI GDGCHC 5 cut(s) 29, 124, 282, 362, 1068
SexAI ACCWGGT 1 cut(s) 246
SfaNI GCATC 3 cut(s) 550, 805, 818
SinI GGWCC 3 cut(s) 136, 395, 587
Sse9I AATT 4 cut(s) 236, 314, 510, 1110
SsiI CCGC 5 cut(s) 11, 87, 415, 434, 872
SspMI CTAG 2 cut(s) 599, 942
SstI GAGCTC 1 cut(s) 124
StyD4I CCNGG 4 cut(s) 153, 246, 288, 706
StyI CCWWGG 2 cut(s) 354, 477
TaaI ACNGT 1 cut(s) 646
TaqI TCGA 3 cut(s) 142, 174, 926
TaqII GACCGA 1 cut(s) 383
TasI AATT 4 cut(s) 236, 314, 510, 1110
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 6 cut(s) 38, 50, 62, 482, 702, 848
Tru1I TTAA 3 cut(s) 429, 680, 714
Tru9I TTAA 3 cut(s) 429, 680, 714
TscAI CASTG 2 cut(s) 990, 1033
TseFI GTSAC 2 cut(s) 256, 614
TseI GCWGC 4 cut(s) 340, 533, 580, 1023
Tsp45I GTSAC 2 cut(s) 256, 614
TspDTI ATGAA 2 cut(s) 474, 677
TspRI CASTG 2 cut(s) 990, 1033
VpaK11BI GGWCC 3 cut(s) 136, 395, 587
XceI RCATGY 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 2 cut(s) 599, 942
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.