Rw1G000540
ERF Family

and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009235
Physical Location & Seq
Forward (+)
878058 .. 880826
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000540.1

Sequence Viewer

Length: 1176 bp
ATGCGAGAGAGAGAGAACCCGAACCCGAAACGGAAAAAAAAGAAAAAAAGAAAAATCTCCCGCCATCCCAAAAACGTTAACAAAAACCCTGAAAAAGTCGGGGTCTGGAATATCAAGGGCAAAATGGAGCTGGCGGTGATAGATCTGAGCCCCTACTTGGAATCGGGATCGGAATTGAAGGAGCTGTGCGGCGAGGTCAGTCGGAGTCTCAGAGAAACCGGAGCTCTCTTAGTCAAGGACCCCCGATGTACCGCCCAGGACAACGATCGCTTCCTCGATATGATGGAGAACTACTTCGCCTCTTCTTCCGACTTCAAGCGACTCCACGAGAGGCCCCAATTGCATTACCAGGTCGGCGTGACTCCCGAGGGCGTGGAAGTGCCCAGAAGCCTGGTTGACGAGGAAATGCAGGAGAAATTGAAGCAAATGCCGAAACAAGTGCAGCCTTCCACTCCCAAGGGCCCTGATTGCAAATGGCGATACATGTGGAGAGTCGGTCCCAGACCATCCAAAACCCGCTTTCAGGAACTTAATGCGGAGCCAGTTGTGCCTGAGGGTTTTCCTGAGTGGAAAGAAACCATGGATTCATGGGGGTACAAGATGATTTCGGCAATTGAGGCTGTTGCTGAAATGGCTGCCATTGGATTTGGTTTGCCAAAGGATGCATTCACTTCCCTTATGCAGCGGGGACCACACCTTCTAGCTCCAACTGGAAGTGACCTTCAGCGTTATGGCCAAGAGGGTACTGTCTTTGCAGGATATCACTATGACCTGAACTTTTTAACTATTCATGGCAGAAGTAGATTCCCAGGGCTTAATATCTGGCTAAGAAATGGGCAAAAAGTTGAAGTGAAGGTTCCTGTCGGATGTCTGCTTATTCAGACTGGAAAGCAGATAGAATGGTTAACCGGTGGAGATTGCATAGCTGGAATGCATGAAGTAGTTGTAACAAACAGGACAACTGATGCAATCAAACTAGCAAGGGAGCAAAATCGCATCTTATGGAGAGTATCTTCTACTTTGTTTGGCCACATAGCATCTGATGCTGTCTTGAAGCCCTTAGGCCACTTTGCCGATTCGCCACTTGCGAGTAAATATCCGCCCATTTATGCAGGGGAGTTTGTTGAACAAGAGCTTTCTGTAATCAATCTCAAAGGAAACAAAGGACAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIOX_N

Protein Analysis

391

Amino Acids

44.28

Weight (kDa)

9.21

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 61, 134, 189, 252, 517, 536, 685, 1100
AclI AACGTT 1 cut(s) 75
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 733, 1027
AcuI CTGAAG 1 cut(s) 707
AfaI GTAC 3 cut(s) 250, 596, 745
AfiI CCNNNNNNNGG 2 cut(s) 157, 523
AflIII ACRYGT 1 cut(s) 483
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 6 cut(s) 178, 316, 421, 848, 1054, 1127
AjnI CCWGG 4 cut(s) 255, 348, 390, 808
AluBI AGCT 6 cut(s) 130, 184, 224, 704, 926, 1135
AluI AGCT 6 cut(s) 130, 184, 224, 704, 926, 1135
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 5 cut(s) 332, 460, 733, 1027, 1063
ApaI GGGCCC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 442, 635, 682
AsiGI ACCGGT 1 cut(s) 908
Asp700I GAANNNNTTC 1 cut(s) 293
AspS9I GGNCC 6 cut(s) 238, 333, 460, 461, 497, 689
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 3 cut(s) 238, 497, 689
AxyI CCTNAGG 2 cut(s) 552, 1060
BaeGI GKGCMC 2 cut(s) 384, 464
BalI TGGCCA 2 cut(s) 735, 1029
BanII GRGCYC 3 cut(s) 152, 226, 464
BauI CACGAG 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 454, 622, 694
BccI CCATC 3 cut(s) 72, 277, 514
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BciT130I CCWGG 4 cut(s) 257, 350, 392, 810
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 2 cut(s) 701, 977
BglII AGATCT 1 cut(s) 142
BisI GCNGC 4 cut(s) 190, 443, 636, 683
BlsI GCNGC 4 cut(s) 191, 444, 637, 684
Bme1390I CCNGG 4 cut(s) 257, 350, 392, 810
Bme18I GGWCC 3 cut(s) 238, 497, 689
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 6 cut(s) 238, 333, 460, 461, 497, 689
BmiI GGNNCC 7 cut(s) 240, 335, 462, 499, 540, 690, 858
BmrFI CCNGG 4 cut(s) 257, 350, 392, 810
BmsI GCATC 5 cut(s) 652, 955, 1005, 1033, 1046
BsaJI CCNNGG 6 cut(s) 255, 366, 456, 579, 808, 809
BsaWI WCCGGW 2 cut(s) 218, 908
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 523
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 3 cut(s) 542, 715, 889
Bse21I CCTNAGG 2 cut(s) 552, 1060
BseBI CCWGG 4 cut(s) 257, 350, 392, 810
BseDI CCNNGG 6 cut(s) 255, 366, 456, 579, 808, 809
BseGI GGATG 4 cut(s) 64, 506, 667, 872
BseLI CCNNNNNNNGG 2 cut(s) 157, 523
BseMII CTCAG 4 cut(s) 137, 223, 543, 555
BseNI ACTGG 3 cut(s) 542, 715, 889
BseSI GKGCMC 2 cut(s) 384, 464
BseXI GCAGC 3 cut(s) 454, 622, 694
BsgI GTGCAG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 5 cut(s) 334, 462, 735, 1029, 1065
BshTI ACCGGT 1 cut(s) 908
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 2 cut(s) 219, 909
BslFI GGGAC 2 cut(s) 483, 702
BslI CCNNNNNNNGG 2 cut(s) 157, 523
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 2 cut(s) 483, 702
BsmI GAATGC 2 cut(s) 665, 936
BsnI GGCC 5 cut(s) 334, 462, 735, 1029, 1065
BsoBI CYCGRG 1 cut(s) 365
Bsp120I GGGCCC 1 cut(s) 460
Bsp1286I GDGCHC 4 cut(s) 152, 226, 384, 464
Bsp143I GATC 3 cut(s) 142, 167, 265
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 8 cut(s) 61, 134, 189, 252, 517, 536, 685, 1100
BspANI GGCC 5 cut(s) 334, 462, 735, 1029, 1065
BspCNI CTCAG 4 cut(s) 138, 222, 544, 556
BspLI GGNNCC 7 cut(s) 240, 335, 462, 499, 540, 690, 858
BspPI GGATC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 3 cut(s) 542, 715, 889
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 6 cut(s) 255, 366, 456, 579, 808, 809
BssMI GATC 3 cut(s) 142, 167, 265
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 2 cut(s) 456, 579
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 4 cut(s) 257, 350, 392, 810
Bst4CI ACNGT 1 cut(s) 748
Bst6I CTCTTC 1 cut(s) 307
BstAPI GCANNNNNTGC 1 cut(s) 1043
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 7 cut(s) 146, 209, 229, 552, 564, 827, 1060
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 4 cut(s) 64, 506, 667, 872
BstKTI GATC 3 cut(s) 145, 170, 268
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 3 cut(s) 142, 167, 265
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 6 cut(s) 340, 468, 547, 617, 632, 1043
BstNI CCWGG 4 cut(s) 257, 350, 392, 810
BstNSI RCATGY 1 cut(s) 487
BstSCI CCNGG 4 cut(s) 255, 348, 390, 808
BstSLI GKGCMC 2 cut(s) 384, 464
BstV1I GCAGC 3 cut(s) 454, 622, 694
BstX2I RGATCY 1 cut(s) 142
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 1 cut(s) 142
Bsu36I CCTNAGG 2 cut(s) 552, 1060
BsuRI GGCC 5 cut(s) 334, 462, 735, 1029, 1065
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 4 cut(s) 64, 506, 667, 872
Cac8I GCNNGC 1 cut(s) 132
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 6 cut(s) 238, 333, 460, 461, 497, 689
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 3 cut(s) 249, 595, 744
CspAI ACCGGT 1 cut(s) 908
CviAII CATG 5 cut(s) 484, 580, 588, 791, 935
CviQI GTAC 3 cut(s) 249, 595, 744
DdeI CTNAG 7 cut(s) 146, 209, 229, 552, 564, 827, 1060
DpnI GATC 3 cut(s) 144, 169, 267
DpnII GATC 3 cut(s) 142, 167, 265
EaeI YGGCCR 2 cut(s) 733, 1027
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
EciI GGCGGA 1 cut(s) 1089
Ecl136II GAGCTC 1 cut(s) 224
Eco130I CCWWGG 2 cut(s) 456, 579
Eco24I GRGCYC 3 cut(s) 152, 226, 464
Eco32I GATATC 1 cut(s) 761
Eco47I GGWCC 3 cut(s) 238, 497, 689
Eco53kI GAGCTC 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 707
Eco81I CCTNAGG 2 cut(s) 552, 1060
Eco88I CYCGRG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 224
EcoO109I RGGNCCY 4 cut(s) 238, 333, 460, 461
EcoRII CCWGG 4 cut(s) 255, 348, 390, 808
EcoRV GATATC 1 cut(s) 761
EcoT14I CCWWGG 2 cut(s) 456, 579
EcoT22I ATGCAT 2 cut(s) 667, 936
EcoT38I GRGCYC 3 cut(s) 152, 226, 464
ErhI CCWWGG 2 cut(s) 456, 579
FaeI CATG 5 cut(s) 487, 583, 591, 794, 938
FalI AAGNNNNNCTT 1 cut(s) 1155
FaqI GGGAC 2 cut(s) 483, 702
FatI CATG 5 cut(s) 483, 579, 587, 790, 934
FauI CCCGC 3 cut(s) 68, 524, 678
Fnu4HI GCNGC 4 cut(s) 190, 443, 636, 683
FokI GGATG 4 cut(s) 51, 493, 674, 879
FriOI GRGCYC 3 cut(s) 152, 226, 464
Fsp4HI GCNGC 4 cut(s) 190, 443, 636, 683
FspBI CTAG 2 cut(s) 701, 977
GluI GCNGC 4 cut(s) 190, 443, 636, 683
HaeIII GGCC 5 cut(s) 334, 462, 735, 1029, 1065
HapII CCGG 2 cut(s) 219, 909
Hin1II CATG 5 cut(s) 487, 583, 591, 794, 938
HincII GTYRAC 3 cut(s) 79, 397, 906
HindII GTYRAC 3 cut(s) 79, 397, 906
HinfI GANTC 8 cut(s) 161, 205, 321, 361, 492, 584, 804, 1076
HpaI GTTAAC 2 cut(s) 79, 906
HpaII CCGG 2 cut(s) 219, 909
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 3 cut(s) 79, 397, 906
Hpy188I TCNGA 8 cut(s) 147, 172, 204, 212, 310, 866, 882, 1042
Hpy188III TCNNGA 6 cut(s) 106, 165, 365, 524, 563, 1051
Hpy8I GTNNAC 3 cut(s) 79, 397, 906
HpyAV CCTTC 5 cut(s) 172, 456, 707, 731, 847
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4IV ACGT 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 6 cut(s) 340, 468, 547, 617, 632, 1043
HpyF3I CTNAG 7 cut(s) 146, 209, 229, 552, 564, 827, 1060
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 5 cut(s) 487, 583, 591, 794, 938
KspAI GTTAAC 2 cut(s) 79, 906
Kzo9I GATC 3 cut(s) 142, 167, 265
LmnI GCTCC 6 cut(s) 127, 181, 221, 538, 709, 985
Lsp1109I GCAGC 3 cut(s) 454, 622, 694
LweI GCATC 5 cut(s) 652, 955, 1005, 1033, 1046
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 2 cut(s) 701, 977
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 358, 716, 946
MalI GATC 3 cut(s) 144, 169, 267
MboI GATC 3 cut(s) 142, 167, 265
MboII GAAGA 3 cut(s) 294, 297, 1005
MfeI CAATTG 2 cut(s) 338, 612
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 4 cut(s) 152, 226, 384, 464
MlsI TGGCCA 2 cut(s) 735, 1029
MluCI AATT 4 cut(s) 173, 338, 416, 612
MluNI TGGCCA 2 cut(s) 735, 1029
MlyI GAGTC 4 cut(s) 214, 315, 355, 501
MmeI TCCRAC 4 cut(s) 182, 333, 731, 844
MnlI CCTC 9 cut(s) 187, 284, 310, 324, 361, 394, 547, 610, 733
Mox20I TGGCCA 2 cut(s) 735, 1029
Mph1103I ATGCAT 2 cut(s) 667, 936
MroXI GAANNNNTTC 1 cut(s) 293
MscI TGGCCA 2 cut(s) 735, 1029
MseI TTAA 5 cut(s) 78, 531, 782, 816, 905
Msp20I TGGCCA 2 cut(s) 735, 1029
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 2 cut(s) 219, 909
MspR9I CCNGG 4 cut(s) 257, 350, 392, 810
MunI CAATTG 2 cut(s) 338, 612
Mva1269I GAATGC 2 cut(s) 665, 936
MvaI CCWGG 4 cut(s) 257, 350, 392, 810
MwoI GCNNNNNNNGC 6 cut(s) 340, 468, 547, 617, 632, 1043
NcoI CCATGG 1 cut(s) 579
NdeII GATC 3 cut(s) 142, 167, 265
NlaIII CATG 5 cut(s) 487, 583, 591, 794, 938
NlaIV GGNNCC 7 cut(s) 240, 335, 462, 499, 540, 690, 858
NmuCI GTSAC 2 cut(s) 358, 716
NsiI ATGCAT 2 cut(s) 667, 936
NspI RCATGY 1 cut(s) 487
PasI CCCWGGG 1 cut(s) 809
PciI ACATGT 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 1085
PctI GAATGC 2 cut(s) 665, 936
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 4 cut(s) 161, 584, 804, 1076
PinAI ACCGGT 1 cut(s) 908
PkrI GCNGC 4 cut(s) 191, 444, 637, 684
Ple19I CGATCG 1 cut(s) 268
PleI GAGTC 4 cut(s) 213, 315, 355, 500
PpsI GAGTC 4 cut(s) 213, 315, 355, 500
PpuMI RGGWCCY 1 cut(s) 238
PscI ACATGT 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 226
Psp1406I AACGTT 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 4 cut(s) 255, 348, 390, 808
PspGI CCWGG 4 cut(s) 255, 348, 390, 808
PspN4I GGNNCC 7 cut(s) 240, 335, 462, 499, 540, 690, 858
PspOMI GGGCCC 1 cut(s) 460
PspPI GGNCC 6 cut(s) 238, 333, 460, 461, 497, 689
PspPPI RGGWCCY 1 cut(s) 238
PsuI RGATCY 1 cut(s) 142
PvuI CGATCG 1 cut(s) 268
RsaI GTAC 3 cut(s) 250, 596, 745
RsaNI GTAC 3 cut(s) 249, 595, 744
SacI GAGCTC 1 cut(s) 226
SaqAI TTAA 5 cut(s) 78, 531, 782, 816, 905
SatI GCNGC 4 cut(s) 190, 443, 636, 683
Sau3AI GATC 3 cut(s) 142, 167, 265
Sau96I GGNCC 6 cut(s) 238, 333, 460, 461, 497, 689
SchI GAGTC 4 cut(s) 214, 315, 355, 501
ScrFI CCNGG 4 cut(s) 257, 350, 392, 810
SduI GDGCHC 4 cut(s) 152, 226, 384, 464
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 5 cut(s) 652, 955, 1005, 1033, 1046
SinI GGWCC 3 cut(s) 238, 497, 689
Sse9I AATT 4 cut(s) 173, 338, 416, 612
SsiI CCGC 8 cut(s) 61, 134, 189, 252, 517, 536, 685, 1100
SspMI CTAG 2 cut(s) 701, 977
SstI GAGCTC 1 cut(s) 226
StyD4I CCNGG 4 cut(s) 255, 348, 390, 808
StyI CCWWGG 2 cut(s) 456, 579
TaaI ACNGT 1 cut(s) 748
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 276
TaqII GACCGA 1 cut(s) 485
TasI AATT 4 cut(s) 173, 338, 416, 612
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 4 cut(s) 161, 584, 804, 1076
Tru1I TTAA 5 cut(s) 78, 531, 782, 816, 905
Tru9I TTAA 5 cut(s) 78, 531, 782, 816, 905
TseFI GTSAC 2 cut(s) 358, 716
TseI GCWGC 3 cut(s) 442, 635, 682
Tsp45I GTSAC 2 cut(s) 358, 716
TspDTI ATGAA 3 cut(s) 576, 779, 951
TspGWI ACGGA 1 cut(s) 46
VpaK11BI GGWCC 3 cut(s) 238, 497, 689
XceI RCATGY 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 2 cut(s) 701, 977
Zsp2I ATGCAT 2 cut(s) 667, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.