pycom03g00150

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011299
Physical Location & Seq
Forward (+)
155371 .. 159394
4024 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00150.3

Sequence Viewer

Length: 726 bp
ATGATGCTTTGCGAATCATTGCGTGATCGAATACAGCCATGGCTTCGTGATTACGATAGGCTTCAATCATTGGCCGTCATTCTCCTCTACATTCAGATTGGGTGCGCATTGGTGGGATCGCTTGGTGCACTGTACACCGGGGTTTTGCTGATAAATCTGGCCATTGCATTGTTCGCCCTCGTCGCAATCGAGAGCAGCAGTCAGAGCCTCGGCCGCACATACGCCGTCCTTCTCGTCTGCGCCATTTTCCTCGATGTCATCTGGTTCATTCTCTTCACTCACGAAATCTGGAACCTTTCTCGTGGAAGTGGGAGTAATACAACTTTGTACATCTTCTCTGTAAAGCTCACTCTGGCCATGCAAATTATCGGCTTTTCCATCAGGTTATCCTCCTCCTTGTTATGGATTCAGATCTACAGGTTGGGCCTTTCCTATGTAGACACTAGTGCAACTCCTAGAGAAGCCGATTTTGATTTAAGAAATAGTTTCTTGAGTCCTACTACCCCCGTTGTACCCAGACAAACTTCAGATTCTAGTGATGCTATAGGAGGCGCTATCTATGACCCCGCGTATTACTCCTCACTCTTTGAAGATGGTCAGGGAAAATATTGCAGTGGCAACAGATCTACTTCTGATGTCGAATCTTCTAAGGCAAAACCATCTGTAGTCAGATCGTTTCAGTTCGTAGATGAGGAGAAGGCAGCAAGCCAGCCTAGTTCGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.56

Weight (kDa)

5.18

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55535 AT1G55535 AT1G55535
fragaria_vesca FvH4_3g45940 FvH4_3g45940
malus_domestica MD03G1001500.v1.1 MD11G1001200.v1.1
prunus_persica Prupe.6G001400_v2.0.a1
pyrus_communis pycom03g00150 pycom11g00140
rosa_chinensis RchiOBHm_Chr5g0084051
rosa_laevigata RLG00000037077
rosa_multiflora Rmu_ssc0000372.1_g000087
rosa_roxburghii Rroxscaffold_1G00000610
rosa_rugosa Rorug05G0488600 Rorug05G0488700
rosa_samantha Rh5AG541300 Rh5BG566600 Rh5CG588600
rosa_wichuraiana Rw5G050340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 106
AccI GTMKAC 1 cut(s) 438
AccII CGCG 1 cut(s) 569
AciI CCGC 2 cut(s) 214, 567
AclWI GGATC 1 cut(s) 124
AcoI YGGCCR 4 cut(s) 72, 159, 211, 354
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 3 cut(s) 134, 329, 513
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 2 cut(s) 65, 590
AhlI ACTAGT 1 cut(s) 443
AluBI AGCT 1 cut(s) 346
AluI AGCT 1 cut(s) 346
Alw21I GWGCWC 1 cut(s) 130
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 1 cut(s) 124
AoxI GGCC 5 cut(s) 72, 159, 211, 354, 424
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 195, 701
AspLEI GCGC 3 cut(s) 107, 242, 554
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 130
BalI TGGCCA 2 cut(s) 161, 356
BauI CACGAG 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 207, 713
BccI CCATC 3 cut(s) 386, 587, 667
BceAI ACGGC 2 cut(s) 59, 209
BcnI CCSGG 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 4 cut(s) 444, 456, 534, 714
BfmI CTRYAG 3 cut(s) 415, 543, 663
BfoI RGCGCY 1 cut(s) 555
BglII AGATCT 2 cut(s) 411, 623
BisI GCNGC 3 cut(s) 196, 214, 702
BlsI GCNGC 3 cut(s) 197, 215, 703
Bme1390I CCNGG 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 293
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 511
BpuMI CCSGG 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 38, 138, 208
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse3DI GCAATG 2 cut(s) 17, 162
Bse8I GATNNNNATC 1 cut(s) 410
BseDI CCNNGG 3 cut(s) 38, 138, 208
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 2 cut(s) 17, 162
BseRI GAGGAG 4 cut(s) 74, 382, 568, 707
BseSI GKGCMC 1 cut(s) 130
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 2 cut(s) 207, 713
Bsh1236I CGCG 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 5 cut(s) 74, 161, 213, 356, 426
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 402
BsnI GGCC 5 cut(s) 74, 161, 213, 356, 426
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 2 cut(s) 132, 327
Bsp143I GATC 5 cut(s) 25, 116, 411, 623, 671
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 2 cut(s) 214, 567
BspANI GGCC 5 cut(s) 74, 161, 213, 356, 426
BspFNI CGCG 1 cut(s) 569
BspLI GGNNCC 1 cut(s) 293
BspPI GGATC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 17, 162
BsrGI TGTACA 2 cut(s) 132, 327
BssECI CCNNGG 3 cut(s) 38, 138, 208
BssMI GATC 5 cut(s) 25, 116, 411, 623, 671
BssSI CACGAG 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 132
Bst6I CTCTTC 1 cut(s) 278
BstAUI TGTACA 2 cut(s) 132, 327
BstC8I GCNNGC 2 cut(s) 706, 710
BstDEI CTNAG 1 cut(s) 648
BstDSI CCRYGG 1 cut(s) 38
BstFNI CGCG 1 cut(s) 569
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 3 cut(s) 107, 242, 554
BstKTI GATC 5 cut(s) 28, 119, 414, 626, 674
BstMBI GATC 5 cut(s) 25, 116, 411, 623, 671
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 4 cut(s) 173, 182, 204, 213
BstSCI CCNGG 1 cut(s) 137
BstSFI CTRYAG 3 cut(s) 415, 543, 663
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 2 cut(s) 207, 713
BstX2I RGATCY 2 cut(s) 411, 623
BstYI RGATCY 2 cut(s) 411, 623
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 5 cut(s) 74, 161, 213, 356, 426
BtgI CCRYGG 1 cut(s) 38
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 128, 619
Cac8I GCNNGC 2 cut(s) 706, 710
CfoI GCGC 3 cut(s) 107, 242, 554
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 3 cut(s) 133, 328, 512
CviAII CATG 2 cut(s) 39, 358
CviQI GTAC 3 cut(s) 133, 328, 512
DdeI CTNAG 1 cut(s) 648
DpnI GATC 5 cut(s) 27, 118, 413, 625, 673
DpnII GATC 5 cut(s) 25, 116, 411, 623, 671
EaeI YGGCCR 4 cut(s) 72, 159, 211, 354
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 38
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 2 cut(s) 42, 361
FaiI YATR 7 cut(s) 40, 220, 359, 403, 435, 545, 561
FatI CATG 2 cut(s) 38, 357
FauI CCCGC 1 cut(s) 574
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 196, 214, 702
Fsp4HI GCNGC 3 cut(s) 196, 214, 702
FspAI RTGCGCAY 1 cut(s) 106
FspBI CTAG 4 cut(s) 444, 456, 534, 714
FspI TGCGCA 1 cut(s) 106
GlaI GCGC 3 cut(s) 106, 241, 553
GluI GCNGC 3 cut(s) 196, 214, 702
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 5 cut(s) 74, 161, 213, 356, 426
HapII CCGG 1 cut(s) 138
HhaI GCGC 3 cut(s) 107, 242, 554
Hin1II CATG 2 cut(s) 42, 361
Hin6I GCGC 3 cut(s) 105, 240, 552
HinP1I GCGC 3 cut(s) 105, 240, 552
HinfI GANTC 5 cut(s) 14, 406, 493, 530, 641
HpaII CCGG 1 cut(s) 138
Hpy166II GTNNAC 3 cut(s) 128, 135, 439
Hpy188I TCNGA 6 cut(s) 96, 204, 411, 529, 634, 671
Hpy188III TCNNGA 5 cut(s) 47, 190, 281, 289, 490
Hpy8I GTNNAC 3 cut(s) 128, 135, 439
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 239, 691
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 128, 167, 361, 449, 612
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 182, 204, 213
HpyF3I CTNAG 1 cut(s) 648
Hsp92II CATG 2 cut(s) 42, 361
HspAI GCGC 3 cut(s) 105, 240, 552
Kzo9I GATC 5 cut(s) 25, 116, 411, 623, 671
Lsp1109I GCAGC 2 cut(s) 207, 713
LweI GCATC 1 cut(s) 529
MaeI CTAG 4 cut(s) 444, 456, 534, 714
MalI GATC 5 cut(s) 27, 118, 413, 625, 673
MboI GATC 5 cut(s) 25, 116, 411, 623, 671
MboII GAAGA 4 cut(s) 265, 325, 602, 636
MflI RGATCY 2 cut(s) 411, 623
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 2 cut(s) 161, 356
MluCI AATT 1 cut(s) 363
MluNI TGGCCA 2 cut(s) 161, 356
MlyI GAGTC 1 cut(s) 502
MnlI CCTC 9 cut(s) 95, 188, 218, 260, 400, 403, 542, 589, 685
Mox20I TGGCCA 2 cut(s) 161, 356
MscI TGGCCA 2 cut(s) 161, 356
MseI TTAA 2 cut(s) 476, 724
Msp20I TGGCCA 2 cut(s) 161, 356
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 4 cut(s) 173, 182, 204, 213
NciI CCSGG 1 cut(s) 139
NcoI CCATGG 1 cut(s) 38
NdeII GATC 5 cut(s) 25, 116, 411, 623, 671
NlaIII CATG 2 cut(s) 42, 361
NlaIV GGNNCC 1 cut(s) 293
NmeAIII GCCGAG 1 cut(s) 189
NsbI TGCGCA 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 4 cut(s) 14, 406, 530, 641
PkrI GCNGC 3 cut(s) 197, 215, 703
PleI GAGTC 1 cut(s) 501
PpsI GAGTC 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 293
PspPI GGNCC 1 cut(s) 424
PsuI RGATCY 2 cut(s) 411, 623
RsaI GTAC 3 cut(s) 134, 329, 513
RsaNI GTAC 3 cut(s) 133, 328, 512
SaqAI TTAA 2 cut(s) 476, 724
SatI GCNGC 3 cut(s) 196, 214, 702
Sau3AI GATC 5 cut(s) 25, 116, 411, 623, 671
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 1 cut(s) 130
SetI ASST 4 cut(s) 297, 348, 386, 422
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 3 cut(s) 415, 543, 663
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 1 cut(s) 363
SsiI CCGC 2 cut(s) 214, 567
SspI AATATT 1 cut(s) 608
SspMI CTAG 4 cut(s) 444, 456, 534, 714
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 1 cut(s) 132
TaqI TCGA 4 cut(s) 28, 189, 252, 639
TasI AATT 1 cut(s) 363
TatI WGTACW 2 cut(s) 132, 327
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 4 cut(s) 14, 406, 530, 641
Tru1I TTAA 2 cut(s) 476, 724
Tru9I TTAA 2 cut(s) 476, 724
TscAI CASTG 2 cut(s) 135, 619
TseI GCWGC 2 cut(s) 195, 701
TspDTI ATGAA 1 cut(s) 256
TspRI CASTG 2 cut(s) 135, 619
VneI GTGCAC 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 4 cut(s) 444, 456, 534, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.