pycom03g08380

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007320
Physical Location & Seq
Forward (+)
6776502 .. 6784194
7693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2616 bp
ATGTCGAGGCGGCTGCGGAAGATGGTCCCCTCACTCGTCCTGCCCAGGAAACCTGTTTTTGCGAGAAACGGATGTACGAGAGCATTCGGGTCGAAAGGTCCAACGAGAGCAAAAGCGCGAACAAAACCTCTCTCTGGACCCGGAGACTCGGAAAGTGATCCGAAAATGGACGACGAATTGCTGGAATTGCAGAGGCAATTCGAGTTCGCACAGCAAGCGAAATCGAGCATCAGATTATCCGATCGAAACGTCGTCGAATTGGTCCAGAAGCTTCAGGAGCTCCACATCATCGACTTCGAGCTCCTCCACACCGTCACCGGCAAAGAATACATTACTCCCGATCAATTGAGGAACGAAATATCGGCGGAGGTCAGTAAACTAGGGCGTGTTTCGTTGATTGACCTTGCGGACACTACAGGAGTGGATTTGTATCATGTTGAGAAGCAAGCTCAGCATGTTGTTTTGGATGATCCGGGGCTTATGTTGATTCAAGGCGAAATAATATCACAGTCTTATTGGGATTCTGTCGCCGAAGAAGTCAATGACAGGCTTCAAGAGTGTAGCCAAATCGCTTTGGCAGAACTTGCCGCGCAGTTGCATGTCAGTTCGGAAATGGTGGCATCTGTGTTGGAGCCCCGCCTTGGGACTTTGGTGAAAGGTAGGCTTGAAGGTGGGCAGTTGTATACTCCTGCATATGTCGCACGGGTTACTGCCATGGTTCGTGGTGCTGCCAGAGGTATCGCAGTTCCCACAAACTTATCATTGTTGTGGAGTTCGTTACAGCAGCTGTTGCAAGAAATGGATGGAGCCAGTGGAGTGGCTGTAGAAGGTTCATTTTTCCAGTCCCTGTTTAATGGACTTGTAAAGGAAGGCGAAATTCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCTAATGTCTTTGCCATTGCTCAAAAGGAATCAATTGATTCTTTCTTTTCACAGAATTCTTTCATTAACTATGATGTTCTGCACAAACTTAGAATTCCTCAGCCCATTCAGTTCTTGCAGTCCAGATATCCAGAAGGCATACCTTTGGTTACTACATTTGTTCACCCCTCAATGATTGAGATGCTTGATGCTGCTACAGAAGATGCTCTCGAACGTGATAGCTGGATCGATTCTCTTTCTATACTCCCAATGTCCTTTGGGTCTCAGGATGCATCTAAACTTTTGTCCCTTTGCCCTTCCATTCAGCAGGGTCTCAAGTCTGATAAAGCAATAATCTTCAGGGAGTCATATGTATTTAGCAGCGGCTTTATCAAGGATGTGTATGATCGCCTCAAGAAAGAAATGGAAACCTTTAGTGTTTCAGTTCCTTCCAGTACTGTGGTGTCAGATGATTTGCGGGAGACAAAAGTTGGTCATGACACAAGCAGGTCGACTGAGTCCAATGAAACTGTTAGTGACAGCAGCAGTAATAAACAGGCAACGGAGAAGGGATCAAAAAAGAAAAAAGGTAGAGGGGCTGGAAGCATGATAACAGGGCCAGCTGAGAGTGAGCTGGACAACCAGGACAATGTTCCTACGAAATCTAAGAAAAACCAAAGGAAAGGCAAGAATACCTCTTCCGGACAGGCCTCAGAGTCAAAAGCAGCTGCTAAGTTGGTAAAAATAAAAGAGGAAAATCTCAATGTCCCTTCAGAAGATTGGGTAATGAATAAGATTACAGCGCTTGTACCTGATTTTGAAGAACAAGGTCCTGACGATCCCCAAACAATTCTTGGACCTTTGGCACACTATTTGAGGCCTAAGTTAATCAACTCCTGGAAGGAGAGAAGAAAGGCGTTGTTTACAGAAAATGCGGAGAGAATGAGGAACTTACTTGATAACTTGCAAAAGAAACTTGATGAGTCTTTCTTAAATATGCAGCTTTATGAAAAGGCTTTAGATTTGTTTGAAGATGACCAATCAACCTCAGTCATTTTTCACCGGCATCTGTTAAGAACAACAGCTACTACTATTGCCGATATGCTTCTTCAAAATTTGGACATGCACAACAAATTGAAGAATGGAGTTGAAGTTGCAGAGCCTCAAATTTCAGAATCTATTTCCCTCAACGCAGGAGAGAGAACTTCCATTGCCAAAAACTTTCCTGGATCTCTTTCAAGCAAGGCTCTTGCTGTAGTTGAAGCATTGGAAGGAAAGCGGGTGGAAACTTTCATGACTGCTCTAAGAGATATTGCAGAAGAGAGCGGCTTGCTCTTGAGAAAGCTTGACAAGAAATTGGAAAGAACGCTTCTGCATTCATATCAGAAGGATTTGGTGTCTCAAGTTTCTGCTGAGACCGATCCAGTTCCTCTTTTGCCAAAAGTTGTCTCCCTGATTTACGTACAGGTTCACCATAAAGCTCTTCAAGCACCTGGAAGGGCCATCGCTGTCGCTGTTTCACGGCTGAAGGATAAACTAGATGAATCAGCCTTCAAGATCCTGACGGATTATCAAACTGCAACAGTGACCCTGTTAACGTTAATATCTGCTGCAAGTGGAGACGAAGAAGATTGTTCATCGGATAGAATTTTGAGCAAAAGGGAGCTTTTGGAGACCCAAATGCCCGCACTCAAAGGCCTGGTCTTGCGCAACTCACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

872

Amino Acids

96.5

Weight (kDa)

5.94

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 59 - 343 6.6e-98 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 360 - 407 1.7e-07 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 616 - 746 1.9e-38 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 751 - 864 2.1e-29 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2600
Acc36I ACCTGC 1 cut(s) 1389
AccBSI CCGCTC 1 cut(s) 2217
AccI GTMKAC 2 cut(s) 683, 1403
AccII CGCG 2 cut(s) 118, 590
AccIII TCCGGA 1 cut(s) 1592
AclI AACGTT 1 cut(s) 2489
AclWI GGATC 9 cut(s) 152, 464, 892, 1145, 1471, 1724, 2128, 2306, 2443
AcsI RAATTY 6 cut(s) 876, 967, 1005, 2005, 2058, 2538
AcuI CTGAAG 4 cut(s) 257, 1234, 1647, 2438
AdeI CACNNNGTG 1 cut(s) 893
AfaI GTAC 4 cut(s) 76, 1348, 1701, 2355
AfeI AGCGCT 1 cut(s) 1695
AfiI CCNNNNNNNGG 3 cut(s) 134, 641, 642
AjnI CCWGG 6 cut(s) 44, 1533, 1787, 2116, 2383, 2589
Alw21I GWGCWC 2 cut(s) 282, 303
Alw26I GTCTC 9 cut(s) 138, 1179, 1229, 1367, 2295, 2300, 2344, 2505, 2558
AlwI GGATC 9 cut(s) 152, 464, 892, 1145, 1471, 1724, 2128, 2306, 2443
AlwNI CAGNNNCTG 2 cut(s) 787, 847
Aor13HI TCCGGA 1 cut(s) 1592
Aor51HI AGCGCT 1 cut(s) 1695
AoxI GGCC 5 cut(s) 1508, 1599, 1769, 2391, 2587
ApoI RAATTY 6 cut(s) 876, 967, 1005, 2005, 2058, 2538
ArsI GACNNNNNNTTYG 2 cut(s) 2577, 2609
Asp700I GAANNNNTTC 1 cut(s) 971
AspLEI GCGC 4 cut(s) 118, 592, 1696, 2601
AspS9I GGNCC 8 cut(s) 25, 98, 137, 262, 1508, 1721, 1748, 2391
AsuC2I CCSGG 2 cut(s) 141, 474
AsuHPI GGTGA 5 cut(s) 307, 664, 1067, 1943, 2354
AvaII GGWCC 6 cut(s) 25, 98, 137, 262, 1721, 1748
BanII GRGCYC 3 cut(s) 282, 303, 636
Bbv12I GWGCWC 2 cut(s) 282, 303
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 9 cut(s) 715, 796, 1090, 1284, 1446, 1606, 1628, 1903, 2488
BccI CCATC 3 cut(s) 16, 797, 2402
BceAI ACGGC 1 cut(s) 2429
BcgI CGANNNNNNTGC 3 cut(s) 29, 72, 106
BciT130I CCWGG 6 cut(s) 46, 1535, 1789, 2118, 2385, 2591
BcnI CCSGG 2 cut(s) 141, 474
BcoDI GTCTC 9 cut(s) 138, 1179, 1229, 1367, 2295, 2300, 2344, 2505, 2558
BfaI CTAG 2 cut(s) 380, 2429
BfmI CTRYAG 4 cut(s) 414, 822, 1107, 2145
BfoI RGCGCY 1 cut(s) 1697
BfuAI ACCTGC 1 cut(s) 1389
BlpI GCTNAGC 1 cut(s) 450
BmcAI AGTACT 1 cut(s) 1348
Bme1390I CCNGG 8 cut(s) 46, 141, 474, 1535, 1789, 2118, 2385, 2591
Bme18I GGWCC 6 cut(s) 25, 98, 137, 262, 1721, 1748
BmgT120I GGNCC 8 cut(s) 25, 98, 137, 262, 1508, 1721, 1748, 2391
BmiI GGNNCC 4 cut(s) 27, 139, 633, 808
BmrFI CCNGG 8 cut(s) 46, 141, 474, 1535, 1789, 2118, 2385, 2591
BmsI GCATC 8 cut(s) 237, 629, 1083, 1090, 1105, 1171, 1193, 1966
Bpu10I CCTNAGC 1 cut(s) 1011
Bpu1102I GCTNAGC 1 cut(s) 450
BpuEI CTTGAG 4 cut(s) 1211, 1289, 2248, 2277
BpuMI CCSGG 2 cut(s) 141, 474
Bsa29I ATCGAT 1 cut(s) 1140
BsaAI YACGTR 1 cut(s) 2353
BsaBI GATNNNNATC 1 cut(s) 429
BsaI GGTCTC 4 cut(s) 1179, 1229, 2300, 2558
BsaJI CCNNGG 4 cut(s) 44, 473, 640, 714
BsaWI WCCGGW 1 cut(s) 1592
BsaXI ACNNNNNCTCC 4 cut(s) 807, 837, 2028, 2058
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 641, 642
Bse118I RCCGGY 2 cut(s) 317, 1953
Bse1I ACTGG 4 cut(s) 810, 841, 1344, 2315
Bse3DI GCAATG 2 cut(s) 927, 2100
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 1592
BseBI CCWGG 6 cut(s) 46, 1535, 1789, 2118, 2385, 2591
BseCI ATCGAT 1 cut(s) 1140
BseDI CCNNGG 4 cut(s) 44, 473, 640, 714
BseGI GGATG 5 cut(s) 77, 472, 808, 1186, 1294
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 134, 641, 642
BseMI GCAATG 2 cut(s) 927, 2100
BseMII CTCAG 8 cut(s) 464, 1025, 1190, 1398, 1506, 1617, 1953, 2295
BseNI ACTGG 4 cut(s) 810, 841, 1344, 2315
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 9 cut(s) 715, 796, 1090, 1284, 1446, 1606, 1628, 1903, 2488
BsgI GTGCAG 2 cut(s) 915, 977
Bsh1236I CGCG 2 cut(s) 118, 590
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 5 cut(s) 1510, 1601, 1771, 2393, 2589
BshVI ATCGAT 1 cut(s) 1140
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 282, 303
BsiSI CCGG 5 cut(s) 141, 318, 473, 1593, 1954
BslFI GGGAC 5 cut(s) 11, 658, 829, 1183, 1643
BslI CCNNNNNNNGG 3 cut(s) 134, 641, 642
BsmAI GTCTC 9 cut(s) 138, 1179, 1229, 1367, 2295, 2300, 2344, 2505, 2558
BsmBI CGTCTC 1 cut(s) 2505
BsmFI GGGAC 5 cut(s) 11, 658, 829, 1183, 1643
BsmI GAATGC 2 cut(s) 83, 2266
BsnI GGCC 5 cut(s) 1510, 1601, 1771, 2393, 2589
Bso31I GGTCTC 4 cut(s) 1179, 1229, 2300, 2558
Bsp1286I GDGCHC 3 cut(s) 282, 303, 636
Bsp13I TCCGGA 1 cut(s) 1592
Bsp1720I GCTNAGC 1 cut(s) 450
Bsp19I CCATGG 1 cut(s) 714
BspANI GGCC 5 cut(s) 1510, 1601, 1771, 2393, 2589
BspCNI CTCAG 8 cut(s) 463, 1024, 1189, 1399, 1507, 1616, 1952, 2296
BspDI ATCGAT 1 cut(s) 1140
BspEI TCCGGA 1 cut(s) 1592
BspFNI CGCG 2 cut(s) 118, 590
BspHI TCATGA 2 cut(s) 1387, 2184
BspLI GGNNCC 4 cut(s) 27, 139, 633, 808
BspMI ACCTGC 1 cut(s) 1389
BspPI GGATC 9 cut(s) 152, 464, 892, 1145, 1471, 1724, 2128, 2306, 2443
BspQI GCTCTTC 1 cut(s) 2379
BspTNI GGTCTC 4 cut(s) 1179, 1229, 2300, 2558
BsrBI CCGCTC 1 cut(s) 2217
BsrDI GCAATG 2 cut(s) 927, 2100
BsrFI RCCGGY 2 cut(s) 317, 1953
BsrI ACTGG 4 cut(s) 810, 841, 1344, 2315
BssAI RCCGGY 2 cut(s) 317, 1953
BssECI CCNNGG 4 cut(s) 44, 473, 640, 714
BssNAI GTATAC 1 cut(s) 684
BssT1I CCWWGG 2 cut(s) 640, 714
Bst1107I GTATAC 1 cut(s) 684
Bst2UI CCWGG 6 cut(s) 46, 1535, 1789, 2118, 2385, 2591
Bst4CI ACNGT 5 cut(s) 313, 510, 1351, 1423, 2476
Bst6I CTCTTC 3 cut(s) 1594, 2205, 2379
BstAPI GCANNNNNTGC 2 cut(s) 584, 790
BstBAI YACGTR 1 cut(s) 2353
BstC8I GCNNGC 5 cut(s) 216, 447, 1512, 2222, 2577
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 5 cut(s) 77, 472, 808, 1186, 1294
BstFNI CGCG 2 cut(s) 118, 590
BstH2I RGCGCY 1 cut(s) 1697
BstHHI GCGC 4 cut(s) 118, 592, 1696, 2601
BstMAI GTCTC 9 cut(s) 138, 1179, 1229, 1367, 2295, 2300, 2344, 2505, 2558
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 8 cut(s) 187, 215, 277, 451, 584, 698, 790, 2378
BstNI CCWGG 6 cut(s) 46, 1535, 1789, 2118, 2385, 2591
BstNSI RCATGY 3 cut(s) 458, 602, 2017
BstSCI CCNGG 8 cut(s) 44, 139, 472, 1533, 1787, 2116, 2383, 2589
BstSFI CTRYAG 4 cut(s) 414, 822, 1107, 2145
BstSNI TACGTA 1 cut(s) 2353
BstUI CGCG 2 cut(s) 118, 590
BstV1I GCAGC 9 cut(s) 715, 796, 1090, 1284, 1446, 1606, 1628, 1903, 2488
BstX2I RGATCY 2 cut(s) 2120, 2448
BstXI CCANNNNNNTGG 2 cut(s) 817, 1351
BstYI RGATCY 2 cut(s) 2120, 2448
BstZ17I GTATAC 1 cut(s) 684
Bsu15I ATCGAT 1 cut(s) 1140
BsuRI GGCC 5 cut(s) 1510, 1601, 1771, 2393, 2589
BsuTUI ATCGAT 1 cut(s) 1140
BtgI CCRYGG 1 cut(s) 714
BtgZI GCGATG 1 cut(s) 2380
BtsCI GGATG 5 cut(s) 77, 472, 808, 1186, 1294
BtsIMutI CAGTG 2 cut(s) 817, 2481
BveI ACCTGC 1 cut(s) 1389
Cac8I GCNNGC 5 cut(s) 216, 447, 1512, 2222, 2577
CaiI CAGNNNCTG 2 cut(s) 787, 847
CciI TCATGA 2 cut(s) 1387, 2184
CfoI GCGC 4 cut(s) 118, 592, 1696, 2601
Cfr10I RCCGGY 2 cut(s) 317, 1953
Cfr13I GGNCC 8 cut(s) 25, 98, 137, 262, 1508, 1721, 1748, 2391
ClaI ATCGAT 1 cut(s) 1140
Csp6I GTAC 4 cut(s) 75, 1347, 1700, 2354
CviAII CATG 8 cut(s) 434, 455, 599, 715, 1388, 1498, 2014, 2185
CviQI GTAC 4 cut(s) 75, 1347, 1700, 2354
DraIII CACNNNGTG 1 cut(s) 893
Eam1104I CTCTTC 3 cut(s) 1594, 2205, 2379
EarI CTCTTC 3 cut(s) 1594, 2205, 2379
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 2 cut(s) 280, 301
Eco105I TACGTA 1 cut(s) 2353
Eco130I CCWWGG 2 cut(s) 640, 714
Eco147I AGGCCT 3 cut(s) 1601, 1771, 2589
Eco24I GRGCYC 3 cut(s) 282, 303, 636
Eco31I GGTCTC 4 cut(s) 1179, 1229, 2300, 2558
Eco32I GATATC 1 cut(s) 1040
Eco47I GGWCC 6 cut(s) 25, 98, 137, 262, 1721, 1748
Eco47III AGCGCT 1 cut(s) 1695
Eco53kI GAGCTC 2 cut(s) 280, 301
Eco57I CTGAAG 4 cut(s) 257, 1234, 1647, 2438
EcoICRI GAGCTC 2 cut(s) 280, 301
EcoO109I RGGNCCY 1 cut(s) 1721
EcoRI GAATTC 2 cut(s) 967, 1005
EcoRII CCWGG 6 cut(s) 44, 1533, 1787, 2116, 2383, 2589
EcoRV GATATC 1 cut(s) 1040
EcoT14I CCWWGG 2 cut(s) 640, 714
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 3 cut(s) 282, 303, 636
ErhI CCWWGG 2 cut(s) 640, 714
Esp3I CGTCTC 1 cut(s) 2505
FaeI CATG 8 cut(s) 437, 458, 602, 718, 1391, 1501, 2017, 2188
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FaqI GGGAC 5 cut(s) 11, 658, 829, 1183, 1643
FatI CATG 8 cut(s) 433, 454, 598, 714, 1387, 1497, 2013, 2184
FauI CCCGC 4 cut(s) 644, 1362, 2163, 2584
FauNDI CATATG 2 cut(s) 694, 1261
FblI GTMKAC 2 cut(s) 683, 1403
FokI GGATG 5 cut(s) 84, 479, 815, 1193, 1301
FriOI GRGCYC 3 cut(s) 282, 303, 636
FspBI CTAG 2 cut(s) 380, 2429
FspI TGCGCA 1 cut(s) 2600
GlaI GCGC 4 cut(s) 117, 591, 1695, 2600
HaeII RGCGCY 1 cut(s) 1697
HaeIII GGCC 5 cut(s) 1510, 1601, 1771, 2393, 2589
HapII CCGG 5 cut(s) 141, 318, 473, 1593, 1954
HhaI GCGC 4 cut(s) 118, 592, 1696, 2601
Hin1II CATG 8 cut(s) 437, 458, 602, 718, 1391, 1501, 2017, 2188
Hin6I GCGC 4 cut(s) 116, 590, 1694, 2599
HinP1I GCGC 4 cut(s) 116, 590, 1694, 2599
HincII GTYRAC 2 cut(s) 1404, 2487
HindII GTYRAC 2 cut(s) 1404, 2487
HindIII AAGCTT 2 cut(s) 269, 2234
HpaI GTTAAC 1 cut(s) 2487
HpaII CCGG 5 cut(s) 141, 318, 473, 1593, 1954
HphI GGTGA 5 cut(s) 307, 664, 1067, 1943, 2354
Hpy166II GTNNAC 7 cut(s) 377, 684, 1075, 1404, 1815, 2362, 2487
Hpy8I GTNNAC 7 cut(s) 377, 684, 1075, 1404, 1815, 2362, 2487
Hpy99I CGWCG 3 cut(s) 176, 254, 257
HpyCH4III ACNGT 5 cut(s) 313, 510, 1351, 1423, 2476
HpyCH4IV ACGT 4 cut(s) 249, 1126, 2352, 2489
HpyF10VI GCNNNNNNNGC 8 cut(s) 187, 215, 277, 451, 584, 698, 790, 2378
HpySE526I ACGT 4 cut(s) 249, 1126, 2352, 2489
Hsp92II CATG 8 cut(s) 437, 458, 602, 718, 1391, 1501, 2017, 2188
HspAI GCGC 4 cut(s) 116, 590, 1694, 2599
Kpn2I TCCGGA 1 cut(s) 1592
KspAI GTTAAC 1 cut(s) 2487
LguI GCTCTTC 1 cut(s) 2379
LmnI GCTCC 6 cut(s) 277, 285, 306, 631, 806, 2554
Lsp1109I GCAGC 9 cut(s) 715, 796, 1090, 1284, 1446, 1606, 1628, 1903, 2488
LweI GCATC 8 cut(s) 237, 629, 1083, 1090, 1105, 1171, 1193, 1966
MaeI CTAG 2 cut(s) 380, 2429
MaeII ACGT 4 cut(s) 249, 1126, 2352, 2489
MaeIII GTNAC 6 cut(s) 313, 706, 777, 1060, 1427, 2476
MbiI CCGCTC 1 cut(s) 2217
MfeI CAATTG 2 cut(s) 344, 945
MflI RGATCY 2 cut(s) 2120, 2448
MhlI GDGCHC 3 cut(s) 282, 303, 636
MlyI GAGTC 5 cut(s) 140, 1265, 1418, 1616, 1883
MmeI TCCRAC 2 cut(s) 125, 609
Mph1103I ATGCAT 1 cut(s) 1186
MroI TCCGGA 1 cut(s) 1592
MroXI GAANNNNTTC 1 cut(s) 971
MseI TTAA 7 cut(s) 852, 978, 1778, 1883, 1964, 2486, 2492
MslI CAYNNNNRTG 1 cut(s) 766
MspA1I CMGCKG 4 cut(s) 787, 1275, 1514, 1619
MspI CCGG 5 cut(s) 141, 318, 473, 1593, 1954
MspR9I CCNGG 8 cut(s) 46, 141, 474, 1535, 1789, 2118, 2385, 2591
MunI CAATTG 2 cut(s) 344, 945
Mva1269I GAATGC 2 cut(s) 83, 2266
MvaI CCWGG 6 cut(s) 46, 1535, 1789, 2118, 2385, 2591
MvnI CGCG 2 cut(s) 118, 590
MwoI GCNNNNNNNGC 8 cut(s) 187, 215, 277, 451, 584, 698, 790, 2378
NciI CCSGG 2 cut(s) 141, 474
NcoI CCATGG 1 cut(s) 714
NdeI CATATG 2 cut(s) 694, 1261
NlaIII CATG 8 cut(s) 437, 458, 602, 718, 1391, 1501, 2017, 2188
NlaIV GGNNCC 4 cut(s) 27, 139, 633, 808
NmuCI GTSAC 3 cut(s) 313, 1427, 2476
NsbI TGCGCA 1 cut(s) 2600
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 3 cut(s) 458, 602, 2017
PagI TCATGA 2 cut(s) 1387, 2184
PceI AGGCCT 3 cut(s) 1601, 1771, 2589
PciSI GCTCTTC 1 cut(s) 2379
PctI GAATGC 2 cut(s) 83, 2266
PdmI GAANNNNTTC 1 cut(s) 971
PfeI GAWTC 7 cut(s) 487, 521, 941, 950, 1142, 2066, 2435
PflFI GACNNNGTC 1 cut(s) 1408
PfoI TCCNGGA 2 cut(s) 1787, 2116
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 5 cut(s) 140, 1264, 1417, 1615, 1882
PpsI GAGTC 5 cut(s) 140, 1264, 1417, 1615, 1882
Ppu21I YACGTR 1 cut(s) 2353
PpuMI RGGWCCY 1 cut(s) 1721
Psp124BI GAGCTC 2 cut(s) 282, 303
Psp1406I AACGTT 1 cut(s) 2489
Psp5II RGGWCCY 1 cut(s) 1721
Psp6I CCWGG 6 cut(s) 44, 1533, 1787, 2116, 2383, 2589
PspGI CCWGG 6 cut(s) 44, 1533, 1787, 2116, 2383, 2589
PspN4I GGNNCC 4 cut(s) 27, 139, 633, 808
PspPI GGNCC 8 cut(s) 25, 98, 137, 262, 1508, 1721, 1748, 2391
PspPPI RGGWCCY 1 cut(s) 1721
PsrI GAACNNNNNNTAC 2 cut(s) 1960, 1992
PstNI CAGNNNCTG 2 cut(s) 787, 847
PsuI RGATCY 2 cut(s) 2120, 2448
PsyI GACNNNGTC 1 cut(s) 1408
PvuI CGATCG 1 cut(s) 244
PvuII CAGCTG 3 cut(s) 787, 1514, 1619
RsaI GTAC 4 cut(s) 76, 1348, 1701, 2355
RsaNI GTAC 4 cut(s) 75, 1347, 1700, 2354
RseI CAYNNNNRTG 1 cut(s) 766
SacI GAGCTC 2 cut(s) 282, 303
SalI GTCGAC 1 cut(s) 1402
SapI GCTCTTC 1 cut(s) 2379
SaqAI TTAA 7 cut(s) 852, 978, 1778, 1883, 1964, 2486, 2492
Sau96I GGNCC 8 cut(s) 25, 98, 137, 262, 1508, 1721, 1748, 2391
ScaI AGTACT 1 cut(s) 1348
SchI GAGTC 5 cut(s) 140, 1265, 1418, 1616, 1883
ScrFI CCNGG 8 cut(s) 46, 141, 474, 1535, 1789, 2118, 2385, 2591
SduI GDGCHC 3 cut(s) 282, 303, 636
SfaNI GCATC 8 cut(s) 237, 629, 1083, 1090, 1105, 1171, 1193, 1966
SfcI CTRYAG 4 cut(s) 414, 822, 1107, 2145
SinI GGWCC 6 cut(s) 25, 98, 137, 262, 1721, 1748
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 4 cut(s) 1226, 1304, 2227, 2292
SmoI CTYRAG 4 cut(s) 1226, 1304, 2227, 2292
SnaBI TACGTA 1 cut(s) 2353
SseBI AGGCCT 3 cut(s) 1601, 1771, 2589
SspMI CTAG 2 cut(s) 380, 2429
SstI GAGCTC 2 cut(s) 282, 303
StuI AGGCCT 3 cut(s) 1601, 1771, 2589
StyD4I CCNGG 8 cut(s) 44, 139, 472, 1533, 1787, 2116, 2383, 2589
StyI CCWWGG 2 cut(s) 640, 714
TaaI ACNGT 5 cut(s) 313, 510, 1351, 1423, 2476
TaiI ACGT 4 cut(s) 252, 1129, 2355, 2492
TaqII GACCGA 1 cut(s) 2324
TatI WGTACW 1 cut(s) 1346
TauI GCSGC 4 cut(s) 13, 590, 1278, 2220
TfiI GAWTC 7 cut(s) 487, 521, 941, 950, 1142, 2066, 2435
Tru1I TTAA 7 cut(s) 852, 978, 1778, 1883, 1964, 2486, 2492
Tru9I TTAA 7 cut(s) 852, 978, 1778, 1883, 1964, 2486, 2492
TscAI CASTG 2 cut(s) 817, 2481
TseFI GTSAC 3 cut(s) 313, 1427, 2476
Tsp45I GTSAC 3 cut(s) 313, 1427, 2476
TspGWI ACGGA 3 cut(s) 84, 1469, 2471
TspRI CASTG 2 cut(s) 817, 2481
Tth111I GACNNNGTC 1 cut(s) 1408
VpaK11BI GGWCC 6 cut(s) 25, 98, 137, 262, 1721, 1748
XapI RAATTY 6 cut(s) 876, 967, 1005, 2005, 2058, 2538
XceI RCATGY 3 cut(s) 458, 602, 2017
XcmI CCANNNNNNNNNTGG 1 cut(s) 1742
XmiI GTMKAC 2 cut(s) 683, 1403
XmnI GAANNNNTTC 1 cut(s) 971
XspI CTAG 2 cut(s) 380, 2429
ZrmI AGTACT 1 cut(s) 1348
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.