pycom03g08700

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004899
Physical Location & Seq
Reverse (-)
7043012 .. 7043828
817 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08700.1

Sequence Viewer

Length: 537 bp
ATGACAGAATATGTTGTAACTCGTTGGTATCGAGCCCCTGAGTTACTACTCAACTGTTCAGAATACACTGCAGCAATTGATATTTGGTCAGTAGGGTGCATCTTCATGGAGATTCTTAGGAGGGAACCACTGTTTCCTGGTAAAGACTATGTTCAGCAGTTGAGTCTCATAACTGAGCTGCTAGGTTCACCAGATGATTCAGATCTTGGCTTTTTAAGAAGTGATAATGCTAGGAAGTATGTTAAGCAGCTCCCACATGTCCCAAAGCAACCCTTTGCCGAGAAAATCCCAAATGTCTCGCCACTGGCAATTGATCTTGCAGAAAGAATGCTAGTTTTTGATCCAAGCAAACGTATTACTGTGGAGGAAGCACTGAACCATCCATTTTTATCAAGTCTTCACGAGATCAATGAGGAGCCTATCTGCCCATCTCCATTCGTCTTTGACTTTGAGCAAGCATCCCTGGATGAAGAAGATATAAAGGAGCTCATATGGAGGGAGTCTCTACACTTCAATCCAGACAATATATTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
Pkinase

Protein Analysis

179

Amino Acids

20.53

Weight (kDa)

4.6

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 130 5.4e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 335
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 1 cut(s) 514
AjnI CCWGG 2 cut(s) 136, 462
AluBI AGCT 3 cut(s) 178, 250, 487
AluI AGCT 3 cut(s) 178, 250, 487
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 3 cut(s) 170, 301, 507
AlwI GGATC 1 cut(s) 335
ApeKI GCWGC 3 cut(s) 71, 178, 247
AsuHPI GGTGA 1 cut(s) 180
BanII GRGCYC 2 cut(s) 37, 489
BauI CACGAG 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 83, 165, 259
BccI CCATC 2 cut(s) 387, 436
BciT130I CCWGG 2 cut(s) 138, 464
BcoDI GTCTC 3 cut(s) 170, 301, 507
BfaI CTAG 3 cut(s) 182, 231, 332
BfmI CTRYAG 1 cut(s) 69
BglII AGATCT 1 cut(s) 202
BisI GCNGC 3 cut(s) 72, 179, 248
BlsI GCNGC 3 cut(s) 73, 180, 249
Bme1390I CCNGG 2 cut(s) 138, 464
BmiI GGNNCC 2 cut(s) 126, 417
BmrFI CCNGG 2 cut(s) 138, 464
BmsI GCATC 2 cut(s) 108, 467
BpiI GAAGAC 1 cut(s) 389
BplI GAGNNNNNCTC 2 cut(s) 487, 519
BsaBI GATNNNNATC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 462
Bse1I ACTGG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 2 cut(s) 138, 464
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 3 cut(s) 379, 458, 472
BseJI GATNNNNATC 1 cut(s) 201
BseMII CTCAG 2 cut(s) 30, 165
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 3 cut(s) 83, 165, 259
BsiHKAI GWGCWC 1 cut(s) 489
BslFI GGGAC 1 cut(s) 245
BsmAI GTCTC 3 cut(s) 170, 301, 507
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 37, 489
Bsp143I GATC 4 cut(s) 202, 313, 340, 405
BspCNI CTCAG 2 cut(s) 31, 166
BspLI GGNNCC 2 cut(s) 126, 417
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 1 cut(s) 335
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 4 cut(s) 202, 313, 340, 405
BssSI CACGAG 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 401
Bst2UI CCWGG 2 cut(s) 138, 464
Bst4CI ACNGT 3 cut(s) 56, 132, 361
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 3 cut(s) 39, 116, 174
BstF5I GGATG 3 cut(s) 379, 458, 472
BstKTI GATC 4 cut(s) 205, 316, 343, 408
BstMAI GTCTC 3 cut(s) 170, 301, 507
BstMBI GATC 4 cut(s) 202, 313, 340, 405
BstNI CCWGG 2 cut(s) 138, 464
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 136, 462
BstSFI CTRYAG 1 cut(s) 69
BstV1I GCAGC 3 cut(s) 83, 165, 259
BstV2I GAAGAC 1 cut(s) 389
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BtsCI GGATG 3 cut(s) 379, 458, 472
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 4 cut(s) 66, 128, 302, 371
Cac8I GCNNGC 1 cut(s) 456
CviAII CATG 2 cut(s) 106, 257
CviJI RGCY 6 cut(s) 35, 178, 210, 250, 418, 487
CviKI_1 RGCY 6 cut(s) 35, 178, 210, 250, 418, 487
DdeI CTNAG 3 cut(s) 39, 116, 174
DpnI GATC 4 cut(s) 204, 315, 342, 407
DpnII GATC 4 cut(s) 202, 313, 340, 405
Ecl136II GAGCTC 1 cut(s) 487
Eco24I GRGCYC 2 cut(s) 37, 489
Eco53kI GAGCTC 1 cut(s) 487
EcoICRI GAGCTC 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 136, 462
EcoT38I GRGCYC 2 cut(s) 37, 489
FaeI CATG 2 cut(s) 109, 260
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 245
FatI CATG 2 cut(s) 105, 256
FauNDI CATATG 1 cut(s) 491
Fnu4HI GCNGC 3 cut(s) 72, 179, 248
FokI GGATG 3 cut(s) 366, 445, 479
FriOI GRGCYC 2 cut(s) 37, 489
Fsp4HI GCNGC 3 cut(s) 72, 179, 248
FspBI CTAG 3 cut(s) 182, 231, 332
GluI GCNGC 3 cut(s) 72, 179, 248
Hin1II CATG 2 cut(s) 109, 260
HinfI GANTC 4 cut(s) 112, 163, 197, 500
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 2 cut(s) 61, 202
Hpy188III TCNNGA 2 cut(s) 401, 518
Hpy8I GTNNAC 1 cut(s) 188
HpyCH4III ACNGT 3 cut(s) 56, 132, 361
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 3 cut(s) 71, 99, 320
HpyF3I CTNAG 3 cut(s) 39, 116, 174
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 2 cut(s) 109, 260
Kzo9I GATC 4 cut(s) 202, 313, 340, 405
LmnI GCTCC 3 cut(s) 255, 415, 484
LpnPI CCDG 8 cut(s) 51, 123, 150, 204, 290, 449, 476, 531
Lsp1109I GCAGC 3 cut(s) 83, 165, 259
LweI GCATC 2 cut(s) 108, 467
MaeI CTAG 3 cut(s) 182, 231, 332
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 2 cut(s) 16, 42
MalI GATC 4 cut(s) 204, 315, 342, 407
MboI GATC 4 cut(s) 202, 313, 340, 405
MboII GAAGA 4 cut(s) 94, 389, 482, 485
MfeI CAATTG 2 cut(s) 75, 309
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 37, 489
MluCI AATT 2 cut(s) 75, 309
MlyI GAGTC 2 cut(s) 172, 509
MnlI CCTC 4 cut(s) 114, 358, 406, 489
MseI TTAA 2 cut(s) 215, 243
MslI CAYNNNNRTG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 138, 464
MunI CAATTG 2 cut(s) 75, 309
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 2 cut(s) 138, 464
NdeI CATATG 1 cut(s) 491
NdeII GATC 4 cut(s) 202, 313, 340, 405
NlaIII CATG 2 cut(s) 109, 260
NlaIV GGNNCC 2 cut(s) 126, 417
NmeAIII GCCGAG 1 cut(s) 304
NspI RCATGY 1 cut(s) 260
PciI ACATGT 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 112, 197
PkrI GCNGC 3 cut(s) 73, 180, 249
PleI GAGTC 2 cut(s) 171, 508
PpsI GAGTC 2 cut(s) 171, 508
PscI ACATGT 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 489
Psp6I CCWGG 2 cut(s) 136, 462
PspGI CCWGG 2 cut(s) 136, 462
PspN4I GGNNCC 2 cut(s) 126, 417
PstI CTGCAG 1 cut(s) 73
PsuI RGATCY 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 104
SacI GAGCTC 1 cut(s) 489
SaqAI TTAA 2 cut(s) 215, 243
SatI GCNGC 3 cut(s) 72, 179, 248
Sau3AI GATC 4 cut(s) 202, 313, 340, 405
SchI GAGTC 2 cut(s) 172, 509
ScrFI CCNGG 2 cut(s) 138, 464
SduI GDGCHC 2 cut(s) 37, 489
SetI ASST 5 cut(s) 180, 187, 252, 355, 489
SfaNI GCATC 2 cut(s) 108, 467
SfcI CTRYAG 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 104
Sse9I AATT 2 cut(s) 75, 309
SspMI CTAG 3 cut(s) 182, 231, 332
SstI GAGCTC 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 136, 462
TaaI ACNGT 3 cut(s) 56, 132, 361
TaiI ACGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 31
TasI AATT 2 cut(s) 75, 309
TfiI GAWTC 2 cut(s) 112, 197
Tru1I TTAA 2 cut(s) 215, 243
Tru9I TTAA 2 cut(s) 215, 243
TscAI CASTG 4 cut(s) 73, 135, 309, 378
TseI GCWGC 3 cut(s) 71, 178, 247
TspDTI ATGAA 2 cut(s) 94, 483
TspRI CASTG 4 cut(s) 73, 135, 309, 378
XceI RCATGY 1 cut(s) 260
XspI CTAG 3 cut(s) 182, 231, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.