Rh5AG404300

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004899
Physical Location & Seq
Forward (+)
70138787 .. 70142582
3796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG404300.1

Sequence Viewer

Length: 1098 bp
ATGGAGAATGGGATGGTTGAAGCTAAACAGCCTTCTGGGTATGTTCAGTACAATCTTCTGGGTAATCTTTTTCAGGTCTCTGCCAAGTATGTCCCTCCTATCAGCCCAGTTGGCCGCGGCGCGTACGGGATTGTTTGCTGTGCAACAAACTCTGAGACAAAAGAAGAGGTTGCAATTAAGAAGATTGGGAATGCATTTGACAATAGGATCGATGCCAAGAGAACGCTCCGAGAGATAAAGCTCCTTAGCCATATGGATCATGACAATATTGTCAAAATTAAGGACATCATAAGGCCACCAGATAAGGAGAATTTCAATGACGTTTACATTGTATATGAATTGATGGACACTGACCTAAATCAGATAATAAACTCCAGCCAGCCTTTGACAGATGATCACTGTCAGTACTTTCTGTATCAATTGCTGCGGGGATTGAAGTACATACACTCTGCGCATGTTTTGCACCGTGATCTGAAACCAAGCAATCTGCTTCTCAATGCAAATTGTGACCTTAAGATTTGTGACTTTGGGCTTGCAAGAACTACATCTGAGACAGATTTCATGACAGAATATGTTGTCACTCGTTGGTATAGAGCCCCAGAATTACTACTCAACTGTTCCGAATACACTGCGGCTATTGATATTTGGTCAGTAGGATGTATTTTCATGGAGATTCTGATGAGGGAACCACTGTTCCCTGGTAAAGACTATGTACAGCAGTTGGGTCTCATAACTGAGCTACTAGGTTCACCGGAAGATTCAAATCTTGGCTTTCTAAGAAGTGAAAATGCTAAGAAGTATGTTAAGCAGCTCCCACATGTCCCAAAGAAACCCTTTGCTCAGAAGTTCCCAAATGTCTCACCAGTGGCAATTGATCTTGCGGAAAAAATGCTAGTTTTTGATCCAAGCAAACGTATAACTGTTGAGGAAGCATTGAATCATCCATATTTATCAAGTCTTCATGCGATCAACGAGGAGCCCATTTGCCCGTCTCCTTTCGTCTTTGATTTTGAGCAGGCATCCCTGGATGAAGAAGACATAAAGGAGCTCGTATGGAGGGAGTCTCTGCACTTCAACCCAGATAATATGCAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
Pkinase

Protein Analysis

365

Amino Acids

41.76

Weight (kDa)

5.18

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 36 - 317 2.4e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 36 - 227 1e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
Acc16I TGCGCA 1 cut(s) 453
AccII CGCG 2 cut(s) 117, 122
AciI CCGC 5 cut(s) 115, 117, 427, 632, 881
AclWI GGATC 3 cut(s) 215, 264, 896
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 310
AfaI GTAC 5 cut(s) 50, 125, 407, 440, 714
AflII CTTAAG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 817
AgsI TTSAA 6 cut(s) 20, 316, 436, 762, 937, 1075
AjnI CCWGG 2 cut(s) 697, 1023
AloI GAACNNNNNNTCC 4 cut(s) 677, 678, 709, 710
AluBI AGCT 5 cut(s) 23, 241, 739, 811, 1048
AluI AGCT 5 cut(s) 23, 241, 739, 811, 1048
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 7 cut(s) 82, 149, 545, 731, 862, 996, 1068
AlwI GGATC 3 cut(s) 215, 264, 896
AoxI GGCC 2 cut(s) 112, 293
ApeKI GCWGC 2 cut(s) 424, 808
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 2 cut(s) 122, 454
AsuHPI GGTGA 2 cut(s) 741, 852
BanII GRGCYC 3 cut(s) 598, 981, 1050
BbsI GAAGAC 2 cut(s) 950, 1041
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 2 cut(s) 411, 820
BccI CCATC 2 cut(s) 7, 337
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 2 cut(s) 699, 1025
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 7 cut(s) 82, 149, 545, 731, 862, 996, 1068
BfaI CTAG 2 cut(s) 743, 893
BfrI CTTAAG 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 5 cut(s) 115, 118, 425, 633, 809
BlsI GCNGC 5 cut(s) 116, 119, 426, 634, 810
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 2 cut(s) 699, 1025
BmiI GGNNCC 2 cut(s) 687, 978
BmrFI CCNGG 2 cut(s) 699, 1025
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 2 cut(s) 202, 1028
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 2 cut(s) 950, 1041
BplI GAGNNNNNCTC 2 cut(s) 1048, 1080
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 245
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 762
BsaI GGTCTC 2 cut(s) 82, 731
BsaJI CCNNGG 3 cut(s) 115, 697, 1023
BsaWI WCCGGW 1 cut(s) 751
Bse1I ACTGG 2 cut(s) 107, 863
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 2 cut(s) 699, 1025
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 115, 697, 1023
BseGI GGATG 5 cut(s) 18, 662, 940, 1019, 1033
BseJI GATNNNNATC 1 cut(s) 762
BseMII CTCAG 4 cut(s) 144, 540, 726, 854
BseNI ACTGG 2 cut(s) 107, 863
BseRI GAGGAG 1 cut(s) 989
BseXI GCAGC 2 cut(s) 411, 820
BsgI GTGCAG 1 cut(s) 1052
Bsh1236I CGCG 2 cut(s) 117, 122
BshFI GGCC 2 cut(s) 114, 295
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 1050
BsiSI CCGG 1 cut(s) 752
BsiWI CGTACG 1 cut(s) 123
BslFI GGGAC 2 cut(s) 77, 806
BsmAI GTCTC 7 cut(s) 82, 149, 545, 731, 862, 996, 1068
BsmBI CGTCTC 1 cut(s) 996
BsmFI GGGAC 2 cut(s) 77, 806
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 2 cut(s) 114, 295
Bso31I GGTCTC 2 cut(s) 82, 731
Bsp1286I GDGCHC 3 cut(s) 598, 981, 1050
Bsp1407I TGTACA 1 cut(s) 712
Bsp143I GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
BspACI CCGC 5 cut(s) 115, 117, 427, 632, 881
BspANI GGCC 2 cut(s) 114, 295
BspCNI CTCAG 4 cut(s) 145, 541, 727, 853
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 2 cut(s) 117, 122
BspHI TCATGA 2 cut(s) 259, 561
BspLI GGNNCC 2 cut(s) 687, 978
BspPI GGATC 3 cut(s) 215, 264, 896
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 2 cut(s) 82, 731
BsrGI TGTACA 1 cut(s) 712
BsrI ACTGG 2 cut(s) 107, 863
BssECI CCNNGG 3 cut(s) 115, 697, 1023
BssMI GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
Bst2UI CCWGG 2 cut(s) 699, 1025
Bst4CI ACNGT 5 cut(s) 401, 467, 617, 693, 922
Bst6I CTCTTC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 460
BstAUI TGTACA 1 cut(s) 712
BstC8I GCNNGC 3 cut(s) 380, 534, 1017
BstDEI CTNAG 7 cut(s) 153, 245, 549, 735, 776, 792, 840
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 5 cut(s) 18, 662, 940, 1019, 1033
BstFNI CGCG 2 cut(s) 117, 122
BstHHI GCGC 2 cut(s) 122, 454
BstKTI GATC 7 cut(s) 210, 259, 397, 472, 877, 904, 969
BstMAI GTCTC 7 cut(s) 82, 149, 545, 731, 862, 996, 1068
BstMBI GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
BstMWI GCNNNNNNNGC 2 cut(s) 111, 460
BstNI CCWGG 2 cut(s) 699, 1025
BstNSI RCATGY 2 cut(s) 458, 821
BstSCI CCNGG 2 cut(s) 697, 1023
BstUI CGCG 2 cut(s) 117, 122
BstV1I GCAGC 2 cut(s) 411, 820
BstV2I GAAGAC 2 cut(s) 950, 1041
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 2 cut(s) 114, 295
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 5 cut(s) 18, 662, 940, 1019, 1033
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 5 cut(s) 348, 397, 627, 689, 870
Cac8I GCNNGC 3 cut(s) 380, 534, 1017
CciI TCATGA 2 cut(s) 259, 561
CfoI GCGC 2 cut(s) 122, 454
Cfr42I CCGCGG 1 cut(s) 118
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 5 cut(s) 49, 124, 406, 439, 713
CviAII CATG 6 cut(s) 260, 455, 562, 667, 818, 962
CviQI GTAC 5 cut(s) 49, 124, 406, 439, 713
DdeI CTNAG 7 cut(s) 153, 245, 549, 735, 776, 792, 840
DpnI GATC 7 cut(s) 209, 258, 396, 471, 876, 903, 968
DpnII GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 1048
Eco24I GRGCYC 3 cut(s) 598, 981, 1050
Eco31I GGTCTC 2 cut(s) 82, 731
Eco53kI GAGCTC 1 cut(s) 1048
EcoICRI GAGCTC 1 cut(s) 1048
EcoRII CCWGG 2 cut(s) 697, 1023
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 3 cut(s) 598, 981, 1050
Esp3I CGTCTC 1 cut(s) 996
FaeI CATG 6 cut(s) 263, 458, 565, 670, 821, 965
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 2 cut(s) 77, 806
FatI CATG 6 cut(s) 259, 454, 561, 666, 817, 961
FauI CCCGC 1 cut(s) 420
FauNDI CATATG 1 cut(s) 252
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 5 cut(s) 115, 118, 425, 633, 809
FokI GGATG 5 cut(s) 25, 669, 927, 1006, 1040
FriOI GRGCYC 3 cut(s) 598, 981, 1050
Fsp4HI GCNGC 5 cut(s) 115, 118, 425, 633, 809
FspBI CTAG 2 cut(s) 743, 893
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 2 cut(s) 121, 453
GluI GCNGC 5 cut(s) 115, 118, 425, 633, 809
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 2 cut(s) 114, 295
HapII CCGG 1 cut(s) 752
HhaI GCGC 2 cut(s) 122, 454
Hin1II CATG 6 cut(s) 263, 458, 565, 670, 821, 965
Hin6I GCGC 2 cut(s) 120, 452
HinP1I GCGC 2 cut(s) 120, 452
HinfI GANTC 4 cut(s) 673, 758, 937, 1061
HpaII CCGG 1 cut(s) 752
HphI GGTGA 2 cut(s) 741, 852
Hpy166II GTNNAC 2 cut(s) 325, 749
Hpy188I TCNGA 8 cut(s) 154, 230, 363, 474, 550, 622, 678, 843
Hpy188III TCNNGA 2 cut(s) 260, 562
Hpy8I GTNNAC 2 cut(s) 325, 749
HpyAV CCTTC 1 cut(s) 42
HpyCH4III ACNGT 5 cut(s) 401, 467, 617, 693, 922
HpyCH4IV ACGT 2 cut(s) 321, 913
HpyCH4V TGCA 8 cut(s) 143, 173, 194, 463, 500, 536, 1069, 1090
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 460
HpyF3I CTNAG 7 cut(s) 153, 245, 549, 735, 776, 792, 840
HpySE526I ACGT 2 cut(s) 321, 913
Hsp92II CATG 6 cut(s) 263, 458, 565, 670, 821, 965
HspAI GCGC 2 cut(s) 120, 452
Ksp22I TGATCA 1 cut(s) 394
KspI CCGCGG 1 cut(s) 118
Kzo9I GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
LmnI GCTCC 5 cut(s) 231, 246, 816, 976, 1045
Lsp1109I GCAGC 2 cut(s) 411, 820
LweI GCATC 2 cut(s) 202, 1028
MaeI CTAG 2 cut(s) 743, 893
MaeII ACGT 2 cut(s) 321, 913
MaeIII GTNAC 3 cut(s) 506, 521, 577
MalI GATC 7 cut(s) 209, 258, 396, 471, 876, 903, 968
MboI GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
MboII GAAGA 7 cut(s) 47, 176, 193, 767, 950, 1043, 1046
MfeI CAATTG 2 cut(s) 419, 870
MhlI GDGCHC 3 cut(s) 598, 981, 1050
MluCI AATT 8 cut(s) 174, 276, 310, 338, 419, 502, 602, 870
MlyI GAGTC 1 cut(s) 1070
MnlI CCTC 6 cut(s) 105, 160, 675, 919, 967, 1050
Mph1103I ATGCAT 1 cut(s) 196
MseI TTAA 4 cut(s) 177, 279, 513, 804
MspA1I CMGCKG 1 cut(s) 117
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 1 cut(s) 752
MspR9I CCNGG 2 cut(s) 699, 1025
MunI CAATTG 2 cut(s) 419, 870
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 2 cut(s) 699, 1025
MvnI CGCG 2 cut(s) 117, 122
MwoI GCNNNNNNNGC 2 cut(s) 111, 460
NdeI CATATG 1 cut(s) 252
NdeII GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
NlaIII CATG 6 cut(s) 263, 458, 565, 670, 821, 965
NlaIV GGNNCC 2 cut(s) 687, 978
NmuCI GTSAC 3 cut(s) 506, 521, 577
NsbI TGCGCA 1 cut(s) 453
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 2 cut(s) 458, 821
PagI TCATGA 2 cut(s) 259, 561
PciI ACATGT 1 cut(s) 817
PctI GAATGC 1 cut(s) 196
PfeI GAWTC 3 cut(s) 673, 758, 937
Pfl23II CGTACG 1 cut(s) 123
PkrI GCNGC 5 cut(s) 116, 119, 426, 634, 810
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
PscI ACATGT 1 cut(s) 817
Psp124BI GAGCTC 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 697, 1023
PspGI CCWGG 2 cut(s) 697, 1023
PspLI CGTACG 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 687, 978
RsaI GTAC 5 cut(s) 50, 125, 407, 440, 714
RsaNI GTAC 5 cut(s) 49, 124, 406, 439, 713
SacI GAGCTC 1 cut(s) 1050
SacII CCGCGG 1 cut(s) 118
SaqAI TTAA 4 cut(s) 177, 279, 513, 804
SatI GCNGC 5 cut(s) 115, 118, 425, 633, 809
Sau3AI GATC 7 cut(s) 207, 256, 394, 469, 874, 901, 966
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 2 cut(s) 699, 1025
SduI GDGCHC 3 cut(s) 598, 981, 1050
SfaNI GCATC 2 cut(s) 202, 1028
Sfr303I CCGCGG 1 cut(s) 118
SgrBI CCGCGG 1 cut(s) 118
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 8 cut(s) 174, 276, 310, 338, 419, 502, 602, 870
SsiI CCGC 5 cut(s) 115, 117, 427, 632, 881
SspI AATATT 1 cut(s) 268
SspMI CTAG 2 cut(s) 743, 893
SstI GAGCTC 1 cut(s) 1050
StyD4I CCNGG 2 cut(s) 697, 1023
TaaI ACNGT 5 cut(s) 401, 467, 617, 693, 922
TaiI ACGT 2 cut(s) 324, 916
TaqI TCGA 1 cut(s) 210
TasI AATT 8 cut(s) 174, 276, 310, 338, 419, 502, 602, 870
TatI WGTACW 4 cut(s) 48, 405, 438, 712
TauI GCSGC 3 cut(s) 117, 120, 635
TfiI GAWTC 3 cut(s) 673, 758, 937
Tru1I TTAA 4 cut(s) 177, 279, 513, 804
Tru9I TTAA 4 cut(s) 177, 279, 513, 804
TscAI CASTG 5 cut(s) 355, 404, 634, 696, 870
TseFI GTSAC 3 cut(s) 506, 521, 577
TseI GCWGC 2 cut(s) 424, 808
Tsp45I GTSAC 3 cut(s) 506, 521, 577
TspDTI ATGAA 5 cut(s) 351, 550, 655, 950, 1044
TspRI CASTG 5 cut(s) 355, 404, 634, 696, 870
Vha464I CTTAAG 1 cut(s) 512
XapI RAATTY 1 cut(s) 310
XceI RCATGY 2 cut(s) 458, 821
XspI CTAG 2 cut(s) 743, 893
ZrmI AGTACT 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.