RchiOBHm_Chr1g0321061

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004899
Physical Location & Seq
Reverse (-)
8509943 .. 8511757
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55114

Sequence Viewer

Length: 450 bp
ATGAATGTTGTGAAACGTGAGCAAGGGACCAATAGTCACTGCACCTATGGGCACGTAATCTTTAATTGTCCCTCCTATCAGCCCAGTTGGCCGCGGCGCGTACGAGATTGTTTGTACTTTCTGTATCAATTGCTGCGGGGATTGAAGTACATACACTCTGCACATGTTTTACACCGTGATCTGAAACTAAGCAATCTGCTTCTCAATGCAAATTGTGACCTTAAGATTTGTGACTTTGGGCTTGCAAGAACTACATCTGAGACAGATTTCATGACAGAATATGTTGTCACTCGTTGGTACCGAGCCCCAGAATTACTACTCAACTGTTCCGAATACACTGCGGCTATTGATATTTGGTCAAATGAACAAAAAGGAGATGGTCCGGGAAGACAAGAAATGGAGGTGTTAAGAAATACAATTGCACAAAGTCTAATGAATGCATCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
Pkinase

Protein Analysis

149

Amino Acids

17.25

Weight (kDa)

7.63

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 34 - 120 8e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 37 - 120 3.1e-30 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 297
AccII CGCG 2 cut(s) 94, 99
AciI CCGC 4 cut(s) 92, 94, 136, 341
AcoI YGGCCR 1 cut(s) 89
AfaI GTAC 4 cut(s) 102, 116, 149, 299
AflII CTTAAG 1 cut(s) 221
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 1 cut(s) 145
AhdI GACNNNNNGTC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 254
AoxI GGCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 133
Asp718I GGTACC 1 cut(s) 297
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 27, 380
AsuC2I CCSGG 1 cut(s) 384
AvaII GGWCC 2 cut(s) 27, 380
BaeGI GKGCMC 1 cut(s) 54
BanI GGYRCC 1 cut(s) 297
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 120
BccI CCATC 1 cut(s) 371
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 254
BfrI CTTAAG 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 88
BisI GCNGC 4 cut(s) 92, 95, 134, 342
BlsI GCNGC 4 cut(s) 93, 96, 135, 343
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 2 cut(s) 27, 380
BmeRI GACNNNNNGTC 1 cut(s) 33
BmgT120I GGNCC 2 cut(s) 27, 380
BmiI GGNNCC 2 cut(s) 28, 299
BmrFI CCNGG 1 cut(s) 384
BmrI ACTGGG 1 cut(s) 78
BmuI ACTGGG 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 394
BpuMI CCSGG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 92
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 1 cut(s) 120
BsgI GTGCAG 2 cut(s) 25, 144
Bsh1236I CGCG 2 cut(s) 94, 99
BshFI GGCC 1 cut(s) 91
BshNI GGYRCC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 383
BsiWI CGTACG 1 cut(s) 100
BslFI GGGAC 2 cut(s) 40, 54
BsmAI GTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 40, 54
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 54, 307
Bsp143I GATC 1 cut(s) 178
BspACI CCGC 4 cut(s) 92, 94, 136, 341
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 1 cut(s) 250
BspFNI CGCG 2 cut(s) 94, 99
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 2 cut(s) 28, 299
BspT107I GGYRCC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 1 cut(s) 178
Bst4CI ACNGT 2 cut(s) 176, 326
BstAFI CTTAAG 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 3 cut(s) 188, 258, 447
BstDSI CCRYGG 1 cut(s) 92
BstFNI CGCG 2 cut(s) 94, 99
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 1 cut(s) 178
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 2 cut(s) 94, 99
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 91
BtgI CCRYGG 1 cut(s) 92
BtsI GCAGTG 2 cut(s) 37, 336
BtsIMutI CAGTG 2 cut(s) 37, 336
Cac8I GCNNGC 1 cut(s) 243
CciI TCATGA 1 cut(s) 270
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 27, 380
Cfr42I CCGCGG 1 cut(s) 95
Csp6I GTAC 4 cut(s) 101, 115, 148, 298
CviAII CATG 2 cut(s) 164, 271
CviJI RGCY 5 cut(s) 82, 91, 241, 305, 344
CviKI_1 RGCY 5 cut(s) 82, 91, 241, 305, 344
CviQI GTAC 4 cut(s) 101, 115, 148, 298
DdeI CTNAG 3 cut(s) 188, 258, 447
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
DriI GACNNNNNGTC 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 27, 380
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 307
FaeI CATG 2 cut(s) 167, 274
FaiI YATR 5 cut(s) 48, 152, 165, 272, 282
FaqI GGGAC 2 cut(s) 40, 54
FatI CATG 2 cut(s) 163, 270
FauI CCCGC 1 cut(s) 129
Fnu4HI GCNGC 4 cut(s) 92, 95, 134, 342
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 4 cut(s) 92, 95, 134, 342
GlaI GCGC 1 cut(s) 98
GluI GCNGC 4 cut(s) 92, 95, 134, 342
HaeIII GGCC 1 cut(s) 91
HapII CCGG 1 cut(s) 383
HhaI GCGC 1 cut(s) 99
Hin1II CATG 2 cut(s) 167, 274
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HpaII CCGG 1 cut(s) 383
Hpy188I TCNGA 3 cut(s) 183, 259, 331
Hpy188III TCNNGA 1 cut(s) 271
HpyCH4III ACNGT 2 cut(s) 176, 326
HpyCH4IV ACGT 2 cut(s) 16, 54
HpyCH4V TGCA 6 cut(s) 42, 161, 209, 245, 422, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 3 cut(s) 188, 258, 447
HpySE526I ACGT 2 cut(s) 16, 54
Hsp92II CATG 2 cut(s) 167, 274
HspAI GCGC 1 cut(s) 97
KpnI GGTACC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 95
Kzo9I GATC 1 cut(s) 178
LpnPI CCDG 3 cut(s) 97, 321, 396
Lsp1109I GCAGC 1 cut(s) 120
MaeII ACGT 2 cut(s) 16, 54
MaeIII GTNAC 4 cut(s) 35, 215, 230, 286
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 1 cut(s) 399
MfeI CAATTG 2 cut(s) 128, 417
MhlI GDGCHC 2 cut(s) 54, 307
MluCI AATT 5 cut(s) 64, 128, 211, 311, 417
MnlI CCTC 2 cut(s) 82, 394
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 3 cut(s) 63, 222, 407
MspA1I CMGCKG 1 cut(s) 94
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 384
MunI CAATTG 2 cut(s) 128, 417
Mva1269I GAATGC 1 cut(s) 442
MvnI CGCG 2 cut(s) 94, 99
MwoI GCNNNNNNNGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 384
NdeII GATC 1 cut(s) 178
NlaIII CATG 2 cut(s) 167, 274
NlaIV GGNNCC 2 cut(s) 28, 299
NmuCI GTSAC 4 cut(s) 35, 215, 230, 286
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 167
PagI TCATGA 1 cut(s) 270
PciI ACATGT 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 298
PctI GAATGC 1 cut(s) 442
Pfl23II CGTACG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 382
PkrI GCNGC 4 cut(s) 93, 96, 135, 343
Ppu21I YACGTR 1 cut(s) 55
PscI ACATGT 1 cut(s) 163
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 28, 299
PspPI GGNCC 2 cut(s) 27, 380
RsaI GTAC 4 cut(s) 102, 116, 149, 299
RsaNI GTAC 4 cut(s) 101, 115, 148, 298
SacII CCGCGG 1 cut(s) 95
SaqAI TTAA 3 cut(s) 63, 222, 407
SatI GCNGC 4 cut(s) 92, 95, 134, 342
Sau3AI GATC 1 cut(s) 178
Sau96I GGNCC 2 cut(s) 27, 380
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 2 cut(s) 54, 307
SetI ASST 5 cut(s) 19, 47, 57, 222, 405
Sfr303I CCGCGG 1 cut(s) 95
SgrBI CCGCGG 1 cut(s) 95
SinI GGWCC 2 cut(s) 27, 380
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 5 cut(s) 64, 128, 211, 311, 417
SsiI CCGC 4 cut(s) 92, 94, 136, 341
StyD4I CCNGG 1 cut(s) 382
TaaI ACNGT 2 cut(s) 176, 326
TaiI ACGT 2 cut(s) 19, 57
TasI AATT 5 cut(s) 64, 128, 211, 311, 417
TatI WGTACW 2 cut(s) 114, 147
TauI GCSGC 3 cut(s) 94, 97, 344
Tru1I TTAA 3 cut(s) 63, 222, 407
Tru9I TTAA 3 cut(s) 63, 222, 407
TscAI CASTG 2 cut(s) 44, 343
TseFI GTSAC 4 cut(s) 35, 215, 230, 286
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 4 cut(s) 35, 215, 230, 286
TspDTI ATGAA 4 cut(s) 17, 259, 378, 449
TspRI CASTG 2 cut(s) 44, 343
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 27, 380
XceI RCATGY 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.