pycom03g10140

N-carbamoylputrescine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006306
Physical Location & Seq
Forward (+)
9206480 .. 9210903
4424 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGTTCGAACATAATCCTCACAGAGAAAAGAAAATGGAGAAGGGCAAGAGGGAGGTGGTGGTCTCAGCTCTGCAGTTCGCTTGCACCGATGACGTCGCCACCAACGTCGCCACCGCCGAAAGGTTGGTTAGAGCTGCCCATGCGAAGGGTGCAAACATCATTCTTATACAGGAACTTTTTGAGGGACATTACTTCTGTCAGGCACAAAGGGAGGATTTCTTTCAGCGAGCAAAGCCTTACAAGGATCATCCAACCATTCTTAGGATGCAAATACTTGCAAAAGAGTTGGGTGTGGTAATACCAGTTAGCTTTTTCGAAGAGGCAAATAATGCTCATTACAATTCAGTTGCCATTATAGATGCTGATGGGGCAGATCTTGGACTTTATAGGAAGTCCCACATCCCAGACGGACCTGGCTACCAGGAAAAGTTCTACTTCAATCCAGGTGACACTGGATTCAAGGTTTTCAAGACCAAATTCGCAACCATTGGAGTTGCAATTTGCTGGGATCAATGGTTCCCTGAGGCTGCCCGGTGTTTGGTTCTTCAAGGTGCAGAGATATTGTTCTACCCAACTGCTATTGGTTCCGAACCTCAAGATGGAGGGCTTGATTCTCGGGATCACTGGAGGCGAGTGATGCAAGGGCATGCTGGGGCTAATGTGGTCCCTGTAGTAACTTCAAATCGTATAGGAAAGGAGATAATCGAGACCGAGCACGGAAAGAGTGAGGTCACTTTCTATGGTAACTCTTTCATAGCAGGACCCACTGGAGAAATTGTTGCAACTGCTAATGATAAAGAGGAAGCTGTACTAGTAGCACAATTTGACCTAGACAAAATCAAATGGAAGAGACATAGCTGGGGAGTATTCCGTGATCGATCGCCGCCCAGATTTATACAAGGTGCTACTGACATCAGATGGCAGCAATTCCCCGTTGTGATGATTCGTTTTGATACCATGCTTGCTCTAGAATTAATAAAGGAAGTACTTCCTCAGTACAATGCGGCCGCGGTGATTTCAAGAGTCCCCAATGGAAGGATTGGAGGATTTACGATTTACTTTAGAGGAAGTATTATTGGCAGGTGA

Protein Analysis

362

Amino Acids

40.62

Weight (kDa)

7.18

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 23 - 280 5.1e-53 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1071
AatII GACGTC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 1071
AccII CGCG 1 cut(s) 1010
AciI CCGC 5 cut(s) 114, 884, 1004, 1008, 1010
AclWI GGATC 3 cut(s) 252, 516, 627
AcoI YGGCCR 1 cut(s) 1005
AcsI RAATTY 1 cut(s) 476
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 3 cut(s) 810, 987, 998
AfiI CCNNNNNNNGG 6 cut(s) 120, 145, 261, 538, 599, 1035
AgsI TTSAA 6 cut(s) 439, 460, 469, 548, 681, 1020
AhlI ACTAGT 1 cut(s) 811
AjnI CCWGG 3 cut(s) 412, 420, 442
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 5 cut(s) 68, 134, 309, 806, 858
AluI AGCT 5 cut(s) 68, 134, 309, 806, 858
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 3 cut(s) 67, 701, 844
AlwI GGATC 3 cut(s) 252, 516, 627
Ama87I CYCGRG 1 cut(s) 615
AoxI GGCC 1 cut(s) 1005
ApeKI GCWGC 3 cut(s) 134, 527, 922
ApoI RAATTY 1 cut(s) 476
AseI ATTAAT 1 cut(s) 974
Asp700I GAANNNNTTC 1 cut(s) 987
AspS9I GGNCC 3 cut(s) 410, 664, 761
AsuC2I CCSGG 1 cut(s) 532
AsuHPI GGTGA 2 cut(s) 458, 1024
AsuII TTCGAA 2 cut(s) 6, 315
AvaI CYCGRG 1 cut(s) 615
AvaII GGWCC 3 cut(s) 410, 664, 761
AxyI CCTNAGG 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 3 cut(s) 121, 514, 934
BccI CCATC 3 cut(s) 359, 593, 912
BciT130I CCWGG 3 cut(s) 414, 422, 444
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 3 cut(s) 67, 701, 844
BcuI ACTAGT 1 cut(s) 811
BfaI CTAG 3 cut(s) 812, 830, 968
BfmI CTRYAG 2 cut(s) 71, 669
BfuAI ACCTGC 1 cut(s) 1071
BglII AGATCT 1 cut(s) 373
BisI GCNGC 6 cut(s) 135, 528, 884, 923, 1005, 1008
BlsI GCNGC 6 cut(s) 136, 529, 885, 924, 1006, 1009
BmcAI AGTACT 1 cut(s) 987
Bme1390I CCNGG 4 cut(s) 414, 422, 444, 532
Bme18I GGWCC 3 cut(s) 410, 664, 761
BmeT110I CYCGRG 1 cut(s) 615
BmgT120I GGNCC 3 cut(s) 410, 664, 761
BmiI GGNNCC 4 cut(s) 518, 586, 666, 763
BmrFI CCNGG 4 cut(s) 414, 422, 444, 532
BmsI GCATC 3 cut(s) 255, 349, 627
BpmI CTGGAG 2 cut(s) 646, 789
Bpu14I TTCGAA 2 cut(s) 6, 315
BpuEI CTTGAG 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 532
Bsa29I ATCGAT 1 cut(s) 877
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 2 cut(s) 67, 701
BsaJI CCNNGG 1 cut(s) 1008
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 6 cut(s) 120, 145, 261, 538, 599, 1035
Bse1I ACTGG 4 cut(s) 302, 457, 629, 772
Bse21I CCTNAGG 1 cut(s) 522
BseBI CCWGG 3 cut(s) 414, 422, 444
BseCI ATCGAT 1 cut(s) 877
BseDI CCNNGG 1 cut(s) 1008
BseGI GGATG 3 cut(s) 247, 270, 399
BseLI CCNNNNNNNGG 6 cut(s) 120, 145, 261, 538, 599, 1035
BseMII CTCAG 3 cut(s) 78, 513, 1007
BseNI ACTGG 4 cut(s) 302, 457, 629, 772
BseX3I CGGCCG 1 cut(s) 1005
BseXI GCAGC 3 cut(s) 121, 514, 934
BseYI CCCAGC 3 cut(s) 504, 650, 858
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 1 cut(s) 1010
Bsh1285I CGRYCG 2 cut(s) 881, 1008
BshFI GGCC 1 cut(s) 1007
BshVI ATCGAT 1 cut(s) 877
BsiEI CGRYCG 2 cut(s) 881, 1008
BsiHKAI GWGCWC 1 cut(s) 717
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 1 cut(s) 532
BslFI GGGAC 4 cut(s) 198, 379, 650, 1010
BslI CCNNNNNNNGG 6 cut(s) 120, 145, 261, 538, 599, 1035
BsmAI GTCTC 3 cut(s) 67, 701, 844
BsmFI GGGAC 4 cut(s) 198, 379, 650, 1010
BsnI GGCC 1 cut(s) 1007
Bso31I GGTCTC 2 cut(s) 67, 701
BsoBI CYCGRG 1 cut(s) 615
Bsp119I TTCGAA 2 cut(s) 6, 315
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 6 cut(s) 244, 373, 508, 619, 874, 878
BspACI CCGC 5 cut(s) 114, 884, 1004, 1008, 1010
BspANI GGCC 1 cut(s) 1007
BspCNI CTCAG 3 cut(s) 77, 514, 1006
BspDI ATCGAT 1 cut(s) 877
BspFNI CGCG 1 cut(s) 1010
BspLI GGNNCC 4 cut(s) 518, 586, 666, 763
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 1071
BspPI GGATC 3 cut(s) 252, 516, 627
BspT104I TTCGAA 2 cut(s) 6, 315
BspTNI GGTCTC 2 cut(s) 67, 701
BsrI ACTGG 4 cut(s) 302, 457, 629, 772
BssECI CCNNGG 1 cut(s) 1008
BssMI GATC 6 cut(s) 244, 373, 508, 619, 874, 878
BssNI GRCGYC 1 cut(s) 93
Bst2UI CCWGG 3 cut(s) 414, 422, 444
Bst6I CTCTTC 2 cut(s) 312, 842
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 329
BstBI TTCGAA 2 cut(s) 6, 315
BstC8I GCNNGC 4 cut(s) 82, 228, 648, 963
BstDEI CTNAG 4 cut(s) 64, 260, 522, 993
BstDSI CCRYGG 1 cut(s) 1008
BstF5I GGATG 3 cut(s) 247, 270, 399
BstFNI CGCG 1 cut(s) 1010
BstKTI GATC 6 cut(s) 247, 376, 511, 622, 877, 881
BstMAI GTCTC 3 cut(s) 67, 701, 844
BstMBI GATC 6 cut(s) 244, 373, 508, 619, 874, 878
BstMCI CGRYCG 2 cut(s) 881, 1008
BstMWI GCNNNNNNNGC 6 cut(s) 140, 149, 232, 329, 368, 637
BstNI CCWGG 3 cut(s) 414, 422, 444
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 4 cut(s) 412, 420, 442, 530
BstSFI CTRYAG 2 cut(s) 71, 669
BstUI CGCG 1 cut(s) 1010
BstV1I GCAGC 3 cut(s) 121, 514, 934
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BstZI CGGCCG 1 cut(s) 1005
Bsu15I ATCGAT 1 cut(s) 877
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 1007
BsuTUI ATCGAT 1 cut(s) 877
BtgI CCRYGG 1 cut(s) 1008
BtsCI GGATG 3 cut(s) 247, 270, 399
BtsIMutI CAGTG 3 cut(s) 450, 622, 765
BveI ACCTGC 1 cut(s) 1071
Cac8I GCNNGC 4 cut(s) 82, 228, 648, 963
CciNI GCGGCCGC 1 cut(s) 1005
Cfr13I GGNCC 3 cut(s) 410, 664, 761
Cfr42I CCGCGG 1 cut(s) 1011
ClaI ATCGAT 1 cut(s) 877
Csp6I GTAC 3 cut(s) 809, 986, 997
CviAII CATG 3 cut(s) 140, 647, 958
CviQI GTAC 3 cut(s) 809, 986, 997
DdeI CTNAG 4 cut(s) 64, 260, 522, 993
DpnI GATC 6 cut(s) 246, 375, 510, 621, 876, 880
DpnII GATC 6 cut(s) 244, 373, 508, 619, 874, 878
EaeI YGGCCR 1 cut(s) 1005
EagI CGGCCG 1 cut(s) 1005
Eam1104I CTCTTC 2 cut(s) 312, 842
EarI CTCTTC 2 cut(s) 312, 842
EclXI CGGCCG 1 cut(s) 1005
Eco31I GGTCTC 2 cut(s) 67, 701
Eco47I GGWCC 3 cut(s) 410, 664, 761
Eco52I CGGCCG 1 cut(s) 1005
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 761
EcoRII CCWGG 3 cut(s) 412, 420, 442
FaeI CATG 3 cut(s) 143, 650, 961
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 4 cut(s) 198, 379, 650, 1010
FatI CATG 3 cut(s) 139, 646, 957
Fnu4HI GCNGC 6 cut(s) 135, 528, 884, 923, 1005, 1008
FokI GGATG 3 cut(s) 234, 277, 386
Fsp4HI GCNGC 6 cut(s) 135, 528, 884, 923, 1005, 1008
FspBI CTAG 3 cut(s) 812, 830, 968
GluI GCNGC 6 cut(s) 135, 528, 884, 923, 1005, 1008
GsaI CCCAGC 3 cut(s) 508, 654, 862
GsuI CTGGAG 2 cut(s) 646, 789
HaeIII GGCC 1 cut(s) 1007
HapII CCGG 1 cut(s) 532
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 3 cut(s) 143, 650, 961
HinfI GANTC 4 cut(s) 456, 611, 943, 1023
HpaII CCGG 1 cut(s) 532
HphI GGTGA 2 cut(s) 458, 1024
Hpy188I TCNGA 2 cut(s) 589, 917
Hpy188III TCNNGA 6 cut(s) 469, 596, 617, 706, 968, 1020
Hpy99I CGWCG 2 cut(s) 98, 110
HpyAV CCTTC 3 cut(s) 34, 139, 1029
HpyCH4IV ACGT 2 cut(s) 93, 105
HpyCH4V TGCA 9 cut(s) 73, 84, 152, 268, 278, 497, 554, 640, 782
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 149, 232, 329, 368, 637
HpyF3I CTNAG 4 cut(s) 64, 260, 522, 993
HpySE526I ACGT 2 cut(s) 93, 105
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 3 cut(s) 143, 650, 961
KspI CCGCGG 1 cut(s) 1011
Kzo9I GATC 6 cut(s) 244, 373, 508, 619, 874, 878
Lsp1109I GCAGC 3 cut(s) 121, 514, 934
LweI GCATC 3 cut(s) 255, 349, 627
MaeI CTAG 3 cut(s) 812, 830, 968
MaeII ACGT 2 cut(s) 93, 105
MaeIII GTNAC 4 cut(s) 446, 673, 730, 743
MalI GATC 6 cut(s) 246, 375, 510, 621, 876, 880
MboI GATC 6 cut(s) 244, 373, 508, 619, 874, 878
MboII GAAGA 3 cut(s) 329, 536, 859
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 7 cut(s) 340, 476, 498, 774, 821, 926, 971
MlyI GAGTC 1 cut(s) 1032
MmeI TCCRAC 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 987
MseI TTAA 1 cut(s) 974
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 1 cut(s) 532
MspR9I CCNGG 4 cut(s) 414, 422, 444, 532
MvaI CCWGG 3 cut(s) 414, 422, 444
MvnI CGCG 1 cut(s) 1010
MwoI GCNNNNNNNGC 6 cut(s) 140, 149, 232, 329, 368, 637
NciI CCSGG 1 cut(s) 532
NdeII GATC 6 cut(s) 244, 373, 508, 619, 874, 878
NlaIII CATG 3 cut(s) 143, 650, 961
NlaIV GGNNCC 4 cut(s) 518, 586, 666, 763
NmuCI GTSAC 2 cut(s) 446, 730
NotI GCGGCCGC 1 cut(s) 1005
NspI RCATGY 1 cut(s) 650
NspV TTCGAA 2 cut(s) 6, 315
PaeI GCATGC 1 cut(s) 650
PaqCI CACCTGC 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 102, 114
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 3 cut(s) 456, 611, 943
PkrI GCNGC 6 cut(s) 136, 529, 885, 924, 1006, 1009
Ple19I CGATCG 1 cut(s) 881
PleI GAGTC 1 cut(s) 1031
PpsI GAGTC 1 cut(s) 1031
PpuMI RGGWCCY 1 cut(s) 761
PshBI ATTAAT 1 cut(s) 974
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 3 cut(s) 412, 420, 442
PspFI CCCAGC 3 cut(s) 504, 650, 858
PspGI CCWGG 3 cut(s) 412, 420, 442
PspN4I GGNNCC 4 cut(s) 518, 586, 666, 763
PspPI GGNCC 3 cut(s) 410, 664, 761
PspPPI RGGWCCY 1 cut(s) 761
PstI CTGCAG 1 cut(s) 75
PsuI RGATCY 1 cut(s) 373
PvuI CGATCG 1 cut(s) 881
RsaI GTAC 3 cut(s) 810, 987, 998
RsaNI GTAC 3 cut(s) 809, 986, 997
SacII CCGCGG 1 cut(s) 1011
SaqAI TTAA 1 cut(s) 974
SatI GCNGC 6 cut(s) 135, 528, 884, 923, 1005, 1008
Sau3AI GATC 6 cut(s) 244, 373, 508, 619, 874, 878
Sau96I GGNCC 3 cut(s) 410, 664, 761
ScaI AGTACT 1 cut(s) 987
SchI GAGTC 1 cut(s) 1032
ScrFI CCNGG 4 cut(s) 414, 422, 444, 532
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 3 cut(s) 255, 349, 627
SfcI CTRYAG 2 cut(s) 71, 669
Sfr303I CCGCGG 1 cut(s) 1011
SfuI TTCGAA 2 cut(s) 6, 315
SgrBI CCGCGG 1 cut(s) 1011
SinI GGWCC 3 cut(s) 410, 664, 761
SmlI CTYRAG 1 cut(s) 594
SmoI CTYRAG 1 cut(s) 594
SpeI ACTAGT 1 cut(s) 811
SphI GCATGC 1 cut(s) 650
Sse9I AATT 7 cut(s) 340, 476, 498, 774, 821, 926, 971
SsiI CCGC 5 cut(s) 114, 884, 1004, 1008, 1010
SspMI CTAG 3 cut(s) 812, 830, 968
StyD4I CCNGG 4 cut(s) 412, 420, 442, 530
TaiI ACGT 2 cut(s) 96, 108
TaqI TCGA 4 cut(s) 6, 315, 705, 877
TaqII GACCGA 1 cut(s) 725
TasI AATT 7 cut(s) 340, 476, 498, 774, 821, 926, 971
TatI WGTACW 3 cut(s) 808, 985, 996
TauI GCSGC 3 cut(s) 886, 1007, 1010
TfiI GAWTC 3 cut(s) 456, 611, 943
Tru1I TTAA 1 cut(s) 974
Tru9I TTAA 1 cut(s) 974
TscAI CASTG 3 cut(s) 457, 629, 772
TseFI GTSAC 2 cut(s) 446, 730
TseI GCWGC 3 cut(s) 134, 527, 922
Tsp45I GTSAC 2 cut(s) 446, 730
TspDTI ATGAA 1 cut(s) 742
TspGWI ACGGA 3 cut(s) 423, 732, 860
TspRI CASTG 3 cut(s) 457, 629, 772
VpaK11BI GGWCC 3 cut(s) 410, 664, 761
VspI ATTAAT 1 cut(s) 974
XapI RAATTY 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 967
XceI RCATGY 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 3 cut(s) 812, 830, 968
ZraI GACGTC 1 cut(s) 94
ZrmI AGTACT 1 cut(s) 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.